Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
...nd VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data on the overa...
Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*
...contribution of internal genes to H5N1 and H7N9 influenza viruses. Multiple H9N2 AIV genotypes have been identified from various species, including birds and mammals. H9N2 AIVs could spread through water, air, and live bird markets. Since the first isolate from 1994 in China, H9N2 AIVs have been prevalent over 20 areas of China, especially in South China, such as Guangdong, Guangxi, and Fujian provinces. Vaccination is the predominant strategy to prevent and c...
Xingdong Yang and Lijuan Yuan
Qingzhong Yu
...E sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression by a NDV vector....
Suresh V Kuchipudi* and Kin-Chow Chang
... response and virus pathogenesis are increasingly recognized. Recent evidence strongly indicated that skeletal muscle cells fully support productive replication of influenza A virus (IAV) and could play an important role in disease outcome. We are of the opinion that skeletal muscle could be a key innate immune tissue/organ in the pathogenesis of influenza and other systemic viral infections which warrants further in-depth i...
Hu Zenglei1 and Xiufan Liu1, 2*
...rtant role in the pathologenesis of NDV, and correlate with the severity of the clinical disease. Virulent strains of different genotypes induce distinct pathological manifestations in chicken organs, especially in lymphoid tissues. Genotype VIId of NDV, which is currently endemic in many countries of Asian and Middle East, replicates at a significantly higher level, induces more potent innate antiviral and inflammatory response, and causes more severe damages...
Shen Yang1, Guang-Zhi Tong1, 2*
...to HP-PRRSV-induced pathogenesis and for the development of novel protective vaccines. In this review, we discuss the innate immune responses against HP-PRRSV infection and suggest new areas of investigation into this important interface between pathogen and host....
Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*
E-mail | [email protected]
...o be applied to JEV pathogenesis and antivirus therapy. This review aims to reveal the exigency of developing a worldwide effort to acknowledge the importance of performing an extensive study of this deadly disease....
Brittany D. Rife 1,2, Marco Salemi 1,2*
E-mail | [email protected]
...viral evolution and pathogenesis....
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
...ive expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several possible improvements in the selection of reference
Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4
...tinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role these wild birds can play in the transmission and evolution dynamics of PPMV-1....
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...d its host-specific pathogenesis. Moreover, understanding the nature of such circulating field viruses is essential to underpin the endemic potential of PPRV and its possible spread to the susceptible wild or domestic small ruminants....
Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1
...dditive and non-additive genes were controlling these characters. The magnitude of SCA variances were higher than GCA indicating pre-dominance of non-additive gene effects for spike length, spike density, seeds spike-1, grain yield plant- and harvest index, while GCA variances were greater for days to 75% maturity, tillers per plant. Parental cultivar TD-1 expressed maximum GCA effects for tillers plant-1, spike length, grain y...
Wan-Long Zhu* and Gao Wenrong
...s in body mass and thermogenesis in Apodemus chevrieri, we examined seasonal-acclimatized and lab-acclimated animals on several physiological, hormonal, and biochemical markers indicative of thermogenic capacity. The results showed that A. chevrieri adapt to winter or under cold condition by increasing thermogenesis and food intake. The present results suggest that the observed physiological regulation fro...
Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*
...ses of 13 protein-coding genes, 22 tRNA genes, two rRNA genes and two control regions CR and CCR, which was first reported in the order Coraciiformes. The overall A+T content for the mitogenome is 52%, and the GC and AT skews are -0.400 and 0.108. Unlike to many other birds, no extra base is inserted at certain position relative to ND3. Interestingly, a 484bp repeated sequence appears in b...
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
...y. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify potential genetic markers for resistance to mastitis. This article presents a systematic integration of complex biological interactions of 226 mammary gland genes to uncover underlying regulatory networks. Data were collected from various databases after havin...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*
...set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii, O. rebecca
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
...tions in GPR54 and GNRHR genes and their correlation with HH in Pakistani boys. Thirty one boys with delayed puberty and thirty one normal age matched controls were examined. Genomic DNA was extracted and amplified by PCR using specific primers for GPR54 and GNRHR splice site exons. Mutations were analyzed by single-stranded conformation polymorphism (SSCP) and/or sequencing. No mutation was identified in GPR54 gene, while two mutations in GNRHR gene were obse...
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
... the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward. This study provides some basic evidence that SS1 may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus....
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
... Calpain and Calpastatin genes and all of their genotype combinations, were also found to be carried by TGC....
Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4
... and six bacterial (Acaligenes, Azotobacter, Bartonella, Nitrsomonas, Pseudomonas and Salmonella) genera were isolated from guano while five fungal (Alternaria, Aspergillus, Chrysosporium, Exophilaand Scopulariopsis) and four bacterial (Bacillus, Corynebacterium, Listeria and Streptomycete) genera were common in bolus and guano samples. Seasonal variations were recorded in occurrence of various fungal and bacterial genera. From bolus samples, two fungal Asperg...
Giselle R.R. Ayres* and Brandão P.E
...on, replication and pathogenesis. The last 3’ one-third of the genome codes the four structural and accessory proteins. Nsps act in a complex replication process, made possible by the special characteristics of AvCoV genome. This manuscript aims to present an overview of the main aspects of the current knowledge on the main AvCoV replicase genes....
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...he regulation of spermatogenesis acting primarily at the hypothalamic level of the gonadotropic axis. Citric acid is synthesized and secreted by the prostate gland. Its synthesis is regulated by androgens. As, dose dependent degeneration of prostate gland has been described following intraperitoneal kisspeptin treatment. However, effects of kisspeptin administration on the levels of prostatic citric acid remain elusive till date. The present study, therefore, ...
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...ting, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary angioplasty (PTCA) as well as translational approaches. In this Experiment, we established a unique murine model of femoral artery injury through a home-made wire. The mouse...
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
...morphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A total of 400 individuals inclu...
Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram
...rich source of resistant genes for stresses including drought and rusts. To evaluate the grain yield and related yield components of synthetic derived bread wheat lines under different moisture levels, this research was conducted during 2006-07 in the field conditions of Tel Hadya and Breda i.e. the leading research venues of International Center for Agricultural Research in the Dry Areas (ICARDA), Syria. The experimental material comprised of forty synthetic ...
Wan-Long Zhu1 and Guang Yang2,*
...pothalamic neuropeptides genes expressions on body mass regulation under different photoperiods in Eothenomys miletus, body mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), Agouti related peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that short photoperiod reduced body mass and body fat mass, and increased food intak...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), representative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the isolate clustered as genotype VII closely associated to those reported from Indonesia. Further sequence analysis of hyper-variable region wi...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...of interferon-stimulated genes (ISGs), responsible for playing an essential and decisive role in the virus pathology. Some studies have already reported the overexpression of these genes against IBDV infection in susceptible cells. The protection ability of ISGs to safeguard chicken cells against the virus could be potentially applied for vaccine production, better vaccination protocols and infection control in the future.
Zafrullah Khan1, Shah Alam Khan1*, Hamayoon Khan2, Naeem Khan3, Khwaja Junaid1 and Inamullah Khan1
...n Pakistan. So far eight genes from the conventional wheat cultivars that are resistant to the attack of R. padi have been incorporated in the commercial wheat cultivars to help manage R. padi. During this study seven wheat genotypes were evaluated as antixenosis, antibiosis and tolerance. These tested genotypes were (Pir Sabaq-2004, Atta-Habib, Saleem-2000, Serin-2010, Khyber-87, Sarhad-82, and Khushal-69) in three separate experiments. Antibios...
Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*
...differentially expressed genes in antler mesenchyme of both sexes. The genes were detected with three anchored primers and ten random primers using differential display reverse transcription PCR (DDRT-PCR). Five differentially expressed fragments were obtained through recovery, cloning and sequencing. Three of them (Af1, Ce1, Ci2) presented a higher expression in antler mesenchyme of female reindeer, but the other two (Ae1, ...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...of antibiotic resistance genes between bacterial spp....
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...ajor features of AD pathogenesis. To provide the basis for further studies with this new animal model, we used high-throughput sequencing to analyze changes in the hippocampal transcriptome induced by injection of the three AD agents. These results open the door to new small-animal studies of AD that complement and extend studies in rodents and primates.
...
Zia Ur Rahman1*, Syed Mehar Ali Shah1, Hidayat Ur Rahman1, Fida Mohammad1, Mian Ahmad Raza1 and Ijaz Ahmad Khan2
...al leaf blight resistant genes into commercial rice cultivars....
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...t-align: justify;">Pseudogenes were previously regarded as molecular fossils, non-functional by-products of genome evolution. However, it has been indicated by several lines of evidences that some pseudogenes are active. Using current data of NCBI we have retrieved 65 pseudogenes from the genome sequence of human pathogenic bacteria Helicobacter pylori (H. pylori) strain 2669...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...tions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya disease of wheat. Currently attempts are being made to utilize RNAi technology to identify the genes targets for the management of root knot and cyst nematodes. In this paper some of the success stories of the technology developed by the scientists at IARI are discussed.
&nbs...
Physiological Responses to Fasting and Refeeding in Apodemus chevrieri from Hengduan Mountain Region
Hai-Ji Zhang1, Di Zhang2, Dong-Min Hou1 and Wan-Long Zhu1,*
...reasing body mass, thermogenesis and serum leptin levels.
...
Yasemin Bircan Yildirim* and Hediye Tugce Vurmay
...pp., Enterobacter aerogenes, Klebsiella pneumoniae, and Enterobacter cloacae were isolated from the gill samples, while E. aerogenes, E. cloacae, Proteus vulgaris, K. pneumoniae and Pseudomonas fluorescens were isolated from the fish intestine samples. E. aerogenes (68.4%), Serratia spp. (53.3%), E. cloacae (50%) and K. pneumoniae ...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
... whole, pave ways to the genesis of cataract.
...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...hemokine receptor (GPCR) genes of two LSDV isolates from Ixodid (hard) ticks (Amblyomma hebraeum) in Egypt. Multiple alignments of the nucleotide sequences revealed that both isolates had nine nucleotide mutations in comparison with the local reference strain, LSDV-Egypt/89 Ismalia. Compared with the GPCR sequences of SPV and GPV strains, 21 nucleotide insertion and 12 nucleotides deletions were identified in the GPCR genes ...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
... analysis exhibited that genes derived from the same GST class from different species share more similarity than genes of different classes in the same species. Copy number of GSTs examining showed that five classes of GST genes in common carp have undergone the gene duplications, including MGST1, GSTK, GSTM, GSTA and GSTT. Comparative genomics and syntenic analysis provided new evidences ...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...pression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the adipose tissue of goat at the puberty onset stage. Moreover, we observed that LEPR, JAK2, STAT3 (P<0.05), KISS1 (P<0.05) were also upregulated in the hypothalamic tissue, except NPY, which was downregulated. Taken together, it is, therefore, inferred that probably leptin secreted in the adipose tissue via its receptor lep...
Muhammad Ashfaq,1 Shamim Akhtar,2 Saleem Akhtar2,* and Mariyam Masood2
...al transcribed spacer I) genes to identify the hyperparasitoid Promuscidea unfasciativentris. The successful PCR-based detection of hyperparasitism is promising for designing strategies for the biological control program of cotton mealybug.
...
Muzafar Shah1,*, Mian Sayed Khan1, Muhammad Ather Rafi2, Habib Ahmed3 and James M. Carpenter4
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...te Antigens (HLA) genes are located on the short arm of the chromosome number 6 and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency of HLA-DRB1*11 and HLA-DRB1*12 allele in aeroallergen sensitized individuals. Blood samples of aeroallergen sensitized individuals were collected at the Allergy Centre, NIH, Islamabad. Then sam...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...ferent stages of spermatogenesis needs further investigation.
...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
...teristics of this family genes. The translation protein of T2R1 gene displayed with corresponding signal loci and functional domains of this gene family, and the result showed that the taste receptor translated by T2R1 gene belongs to G protein-coupled receptor super-family. T. fasciatus taste receptor T2R1 in various tissues appeared differentially expressed. The results showed T2R1 were expressed in gill, spleen, heart, lip...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
... sequence analysis of NA genes shown 99% homology with the H9N2 AIV recently isolated from Pakistan and their phylogenetic analysis revealed that all the isolates belonged to the G- lineage. Amino acid analysis of NA stalk regions of these isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 lineage and human isolates (A/HK/1073/99 and A/HK/1074/99). Several amino acid substitutions at various regions of the NA protein were...
Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...echanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed the omp10 mutant was dramatically attenuated for survival in macrophages and mice than the par...
Muhammad Ibrahim1,2*, Syed Jawad Ahmad Shah1, Shaukat Hussain2, Musharaf Ahmad2 and Farhatullah3
...e yellow rust resistance genes have negligible deployment area which included Tatara (Yr3, Yr5, Yr26), Shafaq-2006 (Yr5, Yr10), Pirsabak-2005 (Yr5, Yr26, Yr30) and Saleem-2000 (Yr5, Yr18, Yr26). Seeds of these cultivars should be increased and deployed on priority bases for yellow rust management in the region. ...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...gion, the control region genes of 20 species were analyzed. All the Ardeidae species had duplicate control regions, except four species having only single region. The control region spans the region between the genes for tRNAGlu - tRNAPhe in the most Ardeidae species. The length of the control region sequences ranged from 1427 bp (Ixobrychus flavicollis) to 3871 bp (Botaurus stellaris). Th...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...and association of these genes with obsessive compulsive disorder (OCD) in the Pakistani Patients. We selected OCD patients (n=100) following the Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) from Sir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore from August 2011 to January 2014. During the sample collection the factors like age/period, employment status and marital status were considered after informed consent. We ...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
... differentiation related genes (Cyp19A, Cyp19B, Arα, ERα, Dax, Foxl2, Dmrt1) and gonad histological changes were examined. The results showed that LET could inhibit oocyte growth in female and promote the development of testes in males. For the gonad differentiation related genes, the expression profiles of Cyp19A and Cyp19B show a pattern of increase in short time and low concentration, but decline in long time ...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
... justify;">Abnormal angiogenesis or the formation of aberrant vasculature in humans, may lead to various pathological conditions including tumor development. The most important factor implicated in the angiogenic processes is vascular endothelial growth factor (VEGF) protein and its family of ligands and receptors (VEGFRs). Survival, proliferation, migration and invasion of endothelial cells which give rise to the vascular network, is mediated through VEGF/VEG...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...mined role in tumor pathogenesis. A total of 52 tumor and normal samples were examined, among these, 40 canine and 12 feline samples were analyzed using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both species. While expression of c-Myc gene was found to be up regulated in different canine (62.5% in mammary adenocar...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...ounts for increased mammogenesis. Bubaline placenta was obtained and processed for extraction of extracellular proteins at pH 9.5 using Tris-Glycine buffer. Placental lactogen was purified by gel filtration chromatography using Sephadex-G100 and ion exchange chromatography on DEAE- Sepharose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactog...
Teresa Napiórkowska* and Julita Templin
...ten days of spider embryogenesis.
...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...n disease and these pathogenesis-related proteins provide valuable information to further study the pathogenesis of popcorn disease and disease-resistant molecular breeding in mulberry.
...
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
...rticipate in the spermatogenesis in the African ostrich. Further studies into this regulation will help clarify the physiological roles played by this hormone in the African ostrich.
...
Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*
...differentially expressed genes in mixed pools of dominant and nondominant follicles of goats. These data will contribute to research on the molecular mechanisms of dominant follicle selection in goats. In this study, 90276370 and 115579236 clear reads in dominant and nondominant follicles of goat were generated through Illumina paired-end sequencing, and their mapping rate was 84.99% and 84.47%, respectively. A total of 12577 differentially expressed
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
...ive virulence factors or genes, including E.coli adherence factor (K88, CS31A, afaE-8), toxins (estA, estB, Stxl, Stx2, EAST1), pathogenicity island (eaeA, irp2, ETT2) and outer membrane protein (ompA). Moreover O-antigen serotype was tested by slide agglutination test and a mouse model was built to assess the lethality of the E.coli isolates through subcutaneous-infection. Out of 81 E.coli isolates, the ...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...ding protein in the pathogenesis of MetS.
...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
... development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analysis was done on miR-122, miR-449a, DUSP4, PDK4 and FKBP1B mRNA expression levels in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition,
Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2
...ial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizophrenic patients and have identified some reasons for disease development. It is estimated from study that in Pakistan there is gender bias in schizophrenia unlike the worldwide data because more of males are affected in the selected family. For the males the age at onset was higher compared to females and the affected ...
Syed Kashif Nawaz*, Faiza Zubair and Sidra Kanwal
...s.
...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
...div>SoxD subgroup genes, which belong to the Sox transcription factor family, have been implicated in the developing nervous system. Their expression has been seen in neural stem cells as well as differentiating neurons. However, despite their importance in development, a relatively low number have been characterized for freshwater fish. In this study, we were able to gain three full-length SoxD sequences from transcriptome sequencing data of car...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
...of the 13 protein coding genes of O. dollfusi began with the unorthodox translation initiation codon ATA and all others use the standard ATG. Ten protein-coding genes use TAA as the termination codon and the rest share the termination codon TAG. There are five cases where tRNA genes appear to overlap. The LNCR of O. dollfusi was 926 nucleotides and no repeated s...
Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal2
...ere governed by additive genes and early phenotypic selection for their improvement might be effective. Five principle components (PCs) were depicted having Eigen value >1 and cumulative variability of 78.66%; PC1 (37.19%), PC2 (12.94%), PC3 (11.62%), PC4 (9.60%) and PC5 (7.32%) of variability. The attributes of significance depicted in PC1 were seed cotton yield, seed index, lint index, sympodia plant-1 and bolls plant-1divulged great contribution towards ...
Aqsa Waqar1, Sahir Hameed Khattak2, Sania Begum2*, Tayyaba Rehman1, Rabia1, Armghan Shehzad2, Wajya Ajmal2, Syeda Shahdana Zia2, Iqra Siddiqi2 and Ghulam Muhammad Ali2*
...race-specific resistance genes. Use of molecular markers is efficient tool for screening diversity of rust genes in wheat germplasm and can facilitate the integration of multiple genes into wheat by pyramiding and transformation. This review discusses information regarding rust disease and resistance in wheat to tackle the disease through resistance breeding. ...
Raania Ahsan* and Zafar Altaf**
...uraged to transfer BtCry genes into such genetic backgrounds which: (i) must have resistance against CLCuV, (ii) are suitable for a given ecology, (iii) meet the set fiber quality parameters (fiber length and strength, GOT% etc.) and (iv) other desirable features required for the release of a normal commercial variety...
Barkat Ali*, Muhammad Shahid Iqbal, Muhammad Kausar Nawaz Shah, Ghulam Shabbir** and Nasir Mahmood Cheema***
...ssessed maximum dominant genes for monopodial branches, plant height and boll weight, BJA-592 for sympodial branches and ACALA-SJ-4 has maximum dominant genes for bolls per plant. However the cultivar CIM-497 carried maximum recessive genes for monopodial branches and plant height, PB-899 for bolls per plant and sympodial branches while ACALA-SJ-4 has maximum recessive
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...on-structural protein 3b genes. Out of the samples collected, 40.17% were positive for CCoV; 57.45% of CCoV-infected animals showed enteritis and most of these (76%) were younger than 3 months and unvaccinated. Distance genealogy using CCoV sequences from GenBank for M gene showed that eight strains were CCoV-II twenty-six were CCoV-I. These findings show some genetic features of CCoV in Brazil and may require future studies to elucidate full genome sequences ...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...ation for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additionally, the shoot proliferation response to increasing level of BAP was found genotype dependent. Naphthaleneacetic acid (NAA) also showed a ...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China ...
Selçuk Kaplan1,* and Sertaç Atalay2
... and leptin (LEP) genes in Kivircik crossbred ewes. Therefore, IGF1/BfoI and Lep/BcnI polymorphisms were examined by polymerase chain reaction- restriction fragment length polymorphism method. The single nucleotide polymorphism (SNP) in the regulatory region of the IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme. Allele frequencies of A and B were found wit...
Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4
...ssbred cows acquire some genes for low physiological response to thermal stress from Bos Indicus cows and acclimatization to the tropical conditions of Pakistan.Therefore, further study is needed on acquisition of genes from Bos Indicus for low physiological response in crossbred cows. ...
Mei Li1, Di Zhang2 and Wan-Long Zhu1,*
...rgy metabolism and thermogenesis, changes in resting metabolic rate (RMR), nonshivering thermogenesis (NST) and energy intake were measured in Eothenomys miletus fed diets containing 0, 3.3% and 6.6% tannic acid, respectively. The results showed that E. miletus fed the diets with 6.6% tannic acid increased RMR compared with control on day 14, and reduced the gross energy intake (GEI) and digestible energy intak...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
... of IL-6, LAMA1 and FGF4 genes did not show any difference between groups. In conclusion, IVM medium supplemented with 10% SFF showed the best rate of blastocyst development.
...
Nadia Faqir1, Aish Muhammad2*, Ghulam Muhammad Ali2, Armghan Shehzad2, Hafeez Ur Rahman3, Farhatullah4 and Muhammad Zeeshan Hyder5
...sequence analysis of two genes (maturase k and geranyl geranyl biphosphate reductase) from chloroplast region. Analysis of variance has revealed significant variation among the studied date palm samples pertaining to the studied physical traits and chemical composition of fruit. Sequences of the maturase k gene has complete identity to the reference genome of ‘Khalas’ cultivar. While geranyl geranyl biphosphate reductase has one synonymous single n...
Shuai Shang1,2, Honghai Zhang2,*, Xiaoyang Wu2, Qinguo Wei2, Jun Chen1, Huanxin Zhang1, Huaming Zhong2 and Xuexi Tang1,*
... receptor (Tas2r) genes has been identified in many species, but the evolution and repertoire of Tas2r genes in raccoon dog (Nyctereutes procyonoides) was still unknown. As a true omnivorous mammal, the Tas2r genes may play a critical role in food selection of raccoon dogs. Thus, we explored the evolution of Tas2r genes...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...of interferon stimulated genes which mediate its anti-proliferative and anti-angiogenic properties. Similarly, Thymosin alpha 1 (Tα-1) helps to fight against different infections such as cancer and hepatitis with its immune modulating properties as well as through its direct action on target cells. The recombinant expression of some proteins in E. coli produces inclusion bodies which are misfolded proteins. The objective of this study was soluble ...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli isolates carried one or more antimic...
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
...tained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 long-noncoding regions [both in the control regions (CR)]. The composition and order of genes in S. lycidas were similar to those of most other invertebrates. The overall base composition of S. lycidas is 35.8% T, 14.8% C, 41.3% A, and 8.1% G, with extremely...
Yijiang Liu1, Kun Li2, Hui Zhang2, Khalid Mehmood2,3, Muhammad Shahzad3, Houqiang Luo1,*, Muhammad Asif Yaseen4 and Xiong Jiang2,5,*
...sed of 13 protein coding genes, 22 tRNA genes and 2 rRNA genes. The ratio of the bases of the mt genome of R. pruinosus from Wenzhou are A (32.31%), T (31.04%), C (24.77%), G (11.88%), A+T (63.35%), respectively. The multivariable sites in rRNA were 1.98% and 5.53% in 12S rRNA and 16S rRNA, respectively. The multivariable sites in protein coding gene were ranged from 0 to 4.8...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
...pp., Listeria monocytogenes, Campylobacter jejuni and E. coli (O157:H7) in various types of food items consumed locally. Total of 800 most common food samples such as chicken, beef meat, raw milk, vegetables and salad samples were collected from retail market, the overall contamination of food samples with infectious agents was 48.37%. Campylobacter jejuni was recorded much higher (28.99%) compared to other foodborne pathogens. Food sample...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
...ps the stages of spermatogenesis appear unaffected, accompanied withimmature and malformed spermatozoa in the lumen.To conclude, oral administration of thiram may affect the seasonal reproduction of pigeon by disturbing the histo-architecture of testes and sperm quality under long photoperiod. Such changes may be responsible for delayed refractory phase.The intact stages of spermatogenesis indicates that the thiram-exposed p...
Selçuk Kaplan
...associations between the genes and economic traits of livestock animals. However, phenotypic values do not generally indicate the real genotypic values of animals. However, using MAS approaches in selection of animals can increase the accuracy of selection. From this point of view, validation of candidate gene markers for marker assisted selection is very important to increase genetic gains in breeding. Leptin is a 16 kDa protein which is highly expressed in a...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...differentially expressed genes (P ≤ 0.05; no fold change). The dynamic impact approach (DIA) bioinformatics tool was used to uncover the most-affected biological pathways among the DEG. Ingenuity pathway analysis (IPA) software also was used to analyze upstream transcription regulators and gene networks. Bioinformatics analysis results indicated that 18 months being a key age for alterations in metabolism. The results of IPA analysis revealed that several t...
Ghulam Abbas1, Muhammad Jawad Asghar1, Muhammad Rizwan2*, Muhammad Akram1, Jaffar Hussain1 and Fiaz Ahmad1
...ment of additive type of genes,and selection based on these traits may help to improve the germplasm. Seed yield showed positive and significant genotypic and phenotypic correlations with clusters per plant (rg = 0.322), pods per plant (rg = 0.276), biological yield (rg = 0.470) and harvest index (rg = 0.264). These traits also showed high positive direct effects on seed yield. Hence,indirect selection for these traits may facilitate for developing high yieldi...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...o copies of somatostatin genes fused to tPA signal sequence was succesfully constructed and identified in MCF-7 cells. Subsequently, thirty female mice randomly were divided into three groups, treating with 50 μg pEGS/2SS-tPA (group T1), 50 μg pEGS/2SS (group T2), and 50 μg pEGFP-N1 (Control group) at weeks 0, 2, 4 and 6. Mating with male mice was performed at 2 weeks post final immunization. Results showed that mice both in T1 and T2 groups el...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...eveloped by chemical mutagenesis. The wild-type was simultaneously treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...the production of fusion genes (BCR-ABL, TEL-AML1, and MLL-AF4). These chromosomal rearrangements have great prognostic and therapeutic significance. Amongst these, TEL-AML1 fusion gene represents a subgroup of ALL cases, which are associated with many clinically important parameters of good prognosis. The objective of this study was to detect the frequency of TEL-AML1 fusion oncogene in ALL and its association with already established prognostic factor...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
... invasion, inducing angiogenesis, inflammation and cellular transformation in cancer. A better understanding into the mechanism of ROS in cancer progression might be useful for the development of biomarkers and therapeutic strategies. The objective of this review was to summarize the roles of ROS in different stages of cancer, cell invasion, angiogenesis and metastasis.
...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
Ansar Ahmed Abbasi1,*, Kathrin Blasius2-4, Imtiaz Ahmed6, Hao Hu7, Sylvie Picker-Minh2-4,8, Muhammad Nasim Khan5, Khalid Hameed1, Aneela Gulnaz1, Zahid Latif5, Abdul Rauf5 and Angela M. Kaindl2-4,8
...l disability. To date 17 genes have been linked with this disease. Biallelic mutations in the abnormal spindle-like, microcephaly associated gene ASPM causes MCPH type 5, the most common MCPH subtype. In the current study we recruited two families from Sialkot region of Pakistan which were assessed by whole exome sequencing and cosegregation analysis. We identified a previously described mutation c.4802C>G (p.S1601*) in the ASPM gene in both f...
G. Venkatesan* and Amit Kumar
...e and specific virulence genes located at termini of ORFV genome with their encoded products are attributed to host immune evasion, short-term immunity both in natural infection post-vaccination in target species and repeated host susceptibility. However, these genes are variable among ORFV isolates/strains as they are not located in central part of genome and some of them are either virus specific namely Viral interferon re...
Jingen Xu1, Yang Liu2, Erhui Jin1, Youfang Gu1, Qin Zhang3 and Shenghe Li1,*
...differentially expressed genes, IL1B, IL17F, IL6R, CXCL2, CCL20 and CCL25 were selected based on their immunological functions and CD8B and CD81 may contribute to variations of blood T lymphocyte subsets. Gene ontology analysis revealed that differential gene expression involved in biological processes, including the G-protein coupled receptor protein signaling pathway, intrinsic to membrane, cell junction and ion transport. KEGG pa...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...-strand-encoding protein genes were conducted by Neighbor-Joining method to reveal the evolutionary relationships within subfamily Schizothoracinae. Three different grades of schizothoracine fishes were well recognized from each other in branching diagram. The primitive group and the specialized group + the highly specialized group constituted a sister relationship with strong supports.
...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...n set of 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and non-coding regions. The mitogenomes of C. mystus N and C. mystus S shared the identical structural organization and gene arrangement with those of other Coilia fishes. Both lineages of C. mystus showeda similar features in not only the strand-specific asymmetry of nucleotide compo...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3,*
...set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii, O. rebecca and Odontamblyopus sp. were all circular double-strand molecules, 17245 bp, 17009 bp and 17004 bp long, respectively. Compared with other bony fishes, the three species shared the similar features in gene arrangements, base composition and tRNA structure. The control region spanned 1571 bp, 1336 bp and 1332...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...ef-8 with other Lef-8genes sequences clearly showed that our SlNPV-FSD15 isolate belongs to Spodoptera litura associated NPVs. The biological activities of this NPV isolates were investigated under laboratory condition. The highest mortality of S. litura was observed at early instars. Against second instars of S. litura, LC50 values of NPV isolate ranged from 1.92×103 to 3.64×103OB/ml...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...ty. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycline, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among
Binbin Shan, Yan Liu, Changping Yang, Shengnan Liu and Dianrong Sun*
...cal alignment, 13,083 unigenes had significant hits in Nr database. And then, 10,467 unigenes were annotated into three ontologies: biological processes, cellular components, and molecular functions. Moreover, 18,621 unigenes were mapped 25 different clusters of eukaryotic proteins. In addition, a total of 17,671 unigenes were classified into 311 KEGG pa...
Asinamai Athliamai Bitrus1*, Olabode Mayowa Peter2, Muhammad Adamu Abbas3 and Mohammed Dauda Goni4
...ickly acquire resistance genes. The development of resistance in bacteria predates the era of antibiotic use. However, resistance developments in S. aureus have been reported since the early 1940-ties, when penicillin resistant S. aureus was first reported. Ever since, this pathogen has gain global notoriety as the most common cause of nosocomial, community and livestock associated infection. The mechanism of resistance development in bacteria involved the int...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...s resistance determinant genes in carbapenems resistant isolates was also evaluated. The carbapenemase and β-lactamases production were evaluated by Modified Hodge test (MHT) and combined disks diffusion (CDD) method, respectively. The MBL producing clinical isolates were further subjected to PCR for the existence of blaIMP and blaVIM genes. The carbapenem resistant A. baumannii
Lamiaa Elsayed Mokhtar Deef
...d mitochondrial 12S rRNA genes, sequences of both genes were placed in the GenBank publication database (Accession numbers are KF783143 and KJ193305) and (Accession numbers are M95109, KF783175, AC175224 and AC160876) of Cyt b and 12S rRNA respectively. Furthermore, DNA barcoding is a widely used molecular-based identification system which aids in identifying species.
...
Yasir Ali1*, Muhammad Aslam Khan1, Muhammad Atiq1 and Muhammad Hussain2
...specific rust resistance genes. Until now, 187 rust resistance genes (80 leaf rust, 49 stripe rust, 58 stem rust) have been described from different wheat and durum cultivars and related local species using different molecular approaches. This review provides a detailed discussion of the different aspects for controlling three rust diseases caused by P. recondita Rob. exdesm f. sp. tritici , P. striiformis West. f. sp. triti...
Hafiz Muhmmad Tahir1*, Rabia Yaqoob2
Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3
Mutation analysis of RING1 domain of Parkin in early onset of Parkinson’s disease in Pakistani patients-a pilot study
... in
men. Almost 9 genes have been reported to be involved in the progression of PD, if mutated. Among them, PARK2
gene is involved in 50% of the early onset cases. Aim of study was to do mutation analysis of RING domain of Parkin
gene as there is no mutation reported previously. In current study, sequence analysis of RING1 domain of Parkin
protein was performed in a sample set of 30 patients (selected from different areas of Punjab,...
men. Almost 9 genes have been reported to be involved in the progression of PD, if mutated. Among them, PARK2
gene is involved in 50% of the early onset cases. Aim of study was to do mutation analysis of RING domain of Parkin
gene as there is no mutation reported previously. In current study, sequence analysis of RING1 domain of Parkin
protein was performed in a sample set of 30 patients (selected from different areas of Punjab,...
Jun Yan Bai1,*, Yong Gang Zhao2 and Yu Qin Wang1
...y is to search candidate genes that control goat growth and explore SNP loci of GHRL genes, thus laying foundations for genetic marker of growth traits of goats. In this study, GHRL genes of black goat and Yaoshan goat were collected for PCR amplification. Polymorphism of goat species was tested by sequencing technology. Results demonstrated that there are two mutation sites (SNP loci) of ...
Shaher Bano1, Sarah Ghafoor1,* and Nadia Naseem2
...udy salivary gland organogenesis. The study is designed to understand T3-mediated regulation of TGF-α expression during postnatal salivary gland development as it is currently unknown.Twelve male rats were taken at week three and twelve at week seven and divided into control and experimental groups. Control rats were given normal saline while experimental rats were given T3 at a dose of 1mg/kg body weight. All the animals were sacrificed on 15th
Bei Liu1, Yanli Hong1, Huifang Zhou1,*, Zhenzhen Cao1, Shuang Zhang1, Jing Jin1, Miao Jiang1, Cunsi Shen2 and Jianjian Ji2
...levels of GnRHR, the hub genes and key transcription factors in the downstream cAMP-PKA signaling pathways in RPC cells were also detected. The secretion and transcription levels of FSH and LH in Cetrorelix group were significantly decreased, while the expression of GnRHR was significantly increased compared to the blank group (p<0.05). Use of CSF with BSZYD alone showed no significant effect on the secretion of RPC, and the expressions of GnRHR and ...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...ighly reported reference genes namely RNU6-2, mir-16-5p and cel-mir-39-1 were selected as the candidate genes for normalization. Preliminary, quantification was performed through real time PCR in eight samples. The geNorm algorithm and comparative delta Cq method were used for selection of suitable reference gene out of the three candidate genes. The validation studies were, thereaf...
Ali Awais, Charassri Nualsri and Watcharin Soonsuwon*
Rizwan Ullah Shah* and Iqbal Munir
...um through somatic embryogenesis (cotyledon and hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four le...
Juan Augusto Hernández-Rivera1, Jaime Molina-Ochoa2,*, Luis Jorge García-Márquez1, Omar Francisco Prado-Rebolledo1, Rafael Julio Macedo-Barragán1, Arturo César García-Casillas1 and Muhammad Irfan Ullah3
...for tolerant heat stress genes and their incorporation in crossbreeding programs has gained interest in dairy cattlemen concerned by the global warming and greenhouse effect in the environment. This review shows and update of the crossbreeding in dairy cattle in both hemispheres. Here, is reviewed the effect of heat stress on dairy cattle; the mechanism of autoregulation; the benefits of the heterosis in crossbreeding cattle by hybridization; the body conditio...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...t be a crucial candidate genes for the selection of duck lipid content.
...
Rishen Liang*, Meng Zhou, Zhenxiang Lin, Guozhang Li, Yuan Chen, Xuan Lin and Zaohe Wu
...ure of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and one noncoding control region. Genomic composition, organization and gene order were similar to that obtained in most vertebrates. By comparative analysis of the two genomes, 941 variable sites (5.69%) were found. Sequence divergences of 13 protein-coding genes, 2 rRNA
Xu Lijie, Obaid Ullah, Liu Haixing, Ihsan Ali, Zhongshu Li* and Nan-Zhu Fang*
...promoters of antioxidant genes and regulates the expression of genes that associate with NADPH oxidase. To understand the role of PPARγ in the development of early embryos and define the mechanism responsible for the arrest in development during the maternal-to-zygotic transition (MZT), we used an embryo model of oxidative damage by H2O2. We found that H2O2 exposure significa...
Ihsan Ullah Khan*, Muhammad Arshad, Muhammad Ayub Khan, Muhammad Ashraf, Ashiq Saleem, Sundas Awan, Samra Azam and Shamim Ul Sibtain Shah
...nd fertility restoration genes gave a new direction to heterosis based sunflower breeding and consequently revolutionized sunflower seed sector. The high impact of heterosis on the yield and other yield contributing traits in the hybrids, as revealed in this study, offers good prospects for the future use of hybrid breeding in sunflower if genetically more diverse germplasm is incorporated in breeding programs. ...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
...st while the tested ESBL genes were detected in 56.52% of isolates. The studyrevealed multidrug resistance phenotypes among K. pneumoniae isolates with higher prevalence of ESBL producing Klebsiella pneumoniae among the children with urinary tract infections. Based on our findings, it is recommended that the ESBL production should be routinely monitored especially among the K. pneumoniae strains for the effective management and cont...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
... evolution of the tested genes is consistent with the evolution of the tested birds. The results of the selection stress test show that only a few sites in the GED region of the Mx gene are positively selected during evolution, and the majority of the amino acid sites are constrained by the strong structure and function of the protein, indicating a high risk of avian influenza infection in the tested wild birds.
...
Fukuan Du1,2, Yongkai Tang1, Juhua Yu1, Shengyan Su1, Fan Yu1, Jianlin Li1, Hongxia Li1, Meiyao Wang1 and Pao Xu1,2,*
.... We obtained 123,764 unigenes with an average length of 1092 bp and a total of 115,169 putative Simple Sequence Repeats (SSRs). Among them, 37 SSRs were selected for the validation experiments. All of the loci were found to be polymorphic and showed bi-allelic in 60 individuals of C. nasus. These SSR markers should not only be useful for population conservation, but also for construction of genetic linkage map and economic performance improvement of
Iram Gull*, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq and Muhammad Amin Athar
...r at junction of spliced genes whenever efficiency of gene fusion is improved by inserting GC rich sequences. In this study, reliable scar-less gene fusion with the insertion of sequence of choice at junction sites has been described by optimization of various parameters of overlap extension PCR. The fusion of IFNα2b gene to the gene of human latency associated protein with insertion of HCV NS3 protease cleavage site at splicing junction is reported.
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV} using mPCR. Resistance for LIN was the highest in overall (96.3%, 77/80) isolates as well as from pig farms (100%, 20/20) and different markets (100%, 20/20, 85%, 17/20). Resistance for other antibiotics such as AMX, TET, AMP and SXT was found 82.5% (66/80), 63.7% (51/80), 58.7% (47/80) and 50% (40/80), respectively. The m...
Syeda Ain-ul-Batool1, Sadia1, Kathrin Blasius2,3,4, Angela Kaindl2,3,4 and Ghazanfar Ali1,*
...ver disorders. So far 21 genes are reported that cause BBS (BBS1-BBS21). A consanguineous family having clinical symptoms of BBS9 is described in current study. Mutation was detected in BBS9 on chromosome 7p14.3 using whole exome sequencing (WES). A splice acceptor site mutation (c.1131G>A) in exon 3 was revealed by Sanger sequencing.
...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
.../div>...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
...les showed that 2,767 unigenes were significantly differentially expressed, including 1,663 genes up-regulated and 1,806 down-regulated in purse red carp. Moreover, gene pathway analysis of the differentially expressed genes was conducted. We proposed the skin color and body shape depended upon at least four pathways including melanin biosynthesis, TGF-beta signaling, Wnt signaling and MAP...
Zhengfei Wang*, Dan Tang, Xuejia Shi, Huayun Guo, Xiuping Chen, Daizheng Zhang and Boping Tang*
...chondrial protein coding genes (PCGs) and adaptation to the extreme hydrothermal vent environment.Thirteen PCGs from mitochondrial genomes of 48 Brachyura species and one Diogenidae species were examined. Each of the genes was investigated and compared to orthologous sequences using PAML, Datamonkey, and TreeSAAP. Nine mitochondrial PCGs (ATP6, ATP8, COX1, COX3, CYTB, ND1, ND2, ND4, and ND5) were validated to h...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
...ion of several proto-oncogenes.
...
Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3*
...FRUITFULL (FUL) MADS-box genes is fundamental to fruit dehiscence zone and valve margin, respectively. The present study was envisaged to isolate the orthologs of SHPs and FUL from local canola “Pakola” and “Punjab Sarsoon 3”, and to study expression patterns of their transcripts. Morphological data revealed significant difference between pod wall thicknesses, seeds per pod and pod length between the two cultivars. PCR amplification and...
Jingchen Chen, Zhaochao Deng and Zhiqiang Han*
...in the 13 protein-coding genes are found. Except for tRNA-Ser(AGN), the second-structure of other tRNAs is the typical clover structure. The lengths of 12S rRNA and 16S rRNA are 945 and 1,698 bp (AP1, AP2) and 1,696 (AP3). A control region containing key sequence tags have three different domains, namely, terminating sequences (TAS1, TAS2), central conservatives (CSB-F, CSB-E and CSB-D) and conservative sequences (CSB1, CSB2 and CSB3). A conserved s...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
... identified in 15,385 unigenes which were generated by Illumina paired-end sequencing in M. fissipes. Within all microsatellites, AG/CT, AAG/CTT, and AAAG/CTTT are most prevalent motif in each repeat class. We randomly selected 61 unigenes with the microsatellite to design primers and do genetic analysis in the Sichuan basin population of M. fissipes. Of all, 35 primer pairs (57.38%) successfully amplificated i...
Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1
...the expression levels of genes that are induced in response to oxidative stress in heart and liver tissues following DOX administration to rats. In this study, DOX was administered to rats intraperitoneally (i. p.) at 3 mg/kg at alternate days for two weeks. Heart and liver tissues were harvested and the mRNA levels of NAD(P)H quinone dehydrogenase 1 (Nqo1), glutathione peroxidase (Gpx1) and isocitrate dehydrogenase-1 (Idh1) were analyzed by RT-PCR. We observe...
Shafqat Husnain Khan1, Shah Jahan2, Irshad Ahmad3,4, Sadeeq ur Rahman5,* and Tayyab ur Rehman4,*
...>VIM encoding genes. Results of modified hodge test identified 81% carbapenemase producers, while combined disc diffusion test identified 28 isolates as MBL producers among them. Of all carbapenemase producers, 25 isolates (30.8%) of carbapenemase-producing E. coli were found to harbor bla-VIM gene, while 13 isolates (16.04%) were found to carry blaIMP gene. We report on high incidence rate of carbapenemase...
Khalid P. Loneand Irfana Liaqat*
...egression of the spermatogenesis. This detailed study is the first from the Northern latitudes of the Indo-Pak subcontinent on the fish kept in commercial ponds.
...
De-Yong Zhang1, Xiao-Lu Xu1,*, Qin Ruan2, Xiu-Ying Shen3 and Yin Lu1
... observation and spermatogenesis evaluation. PFOS ≥ 11 mg/kg (accumulative dosage) resulted in visible histopathological changing in testis, including disorder and vacuolization of spermatogenic cells. Changed testicular organ coefficient (for dosage ≥ 55 mg/kg), decreased sperm concentration (for 110 mg/kg dosage), decreased motility (for 110 mg/kg dosage) and increased sperm malformation (for all of the treated groups) were also confirmed (p<...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
...to detect drug resistant genes like “blaSHV” and “blaOXA” among gram negative rods (GNRs) isolated from different clinical specimens, collected from Jinnah Hospital Lahore during January-December 2015. Majority of the GNRs were isolated from urine samples (55%) followed by pus (28%), blood (7%), sputum (6%), Broncho-alveolar lavage (2%), fluid (1%) and from tips (1%). The isolated organisms were Es...
Uzma Rafi*, Asmat, Muqaddes Niaz, Syeda Shazia Bokhari and Aisha Waheed Qurashi*
...e) and IM2 (Alkaligenes faecalis) were isolated from external surface and rectum portion of cockroach, respectively. Bacterial autoaggregation started increasing after 1 h of culture incubation and reached maximum after 4 h. Isolate CXA1 showed maximum (136 %) auto aggregation as compared to isolate IM2 (37 %) after 4 h. However, biofilm formation in the isolate IM2 was higher as compared to isolate CXA1 at non shaking conditions. The study sh...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...e whole process of embryogenesis and folliculogenesis, early high level and then down regulated. It was significantly expressed higher level in COCs of middle diameter sized follicles than that of small and large sized follicles. BMP15 gene expression enhanced until morula stage but it fell sharply at blastula stage. Immunohistochemistry exhibited BMP15 protein was located in germ cells of testis, in primordial...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...genetic polymorphisms in genes enable us to estimate biological similarities and obtain a better perspective of quantitative traits that play key roles in development and regulating growth. The present study was conducted to characterize genetic variability in the growth hormone (GH) and growth hormone receptor (GHR) of Camelus dromedarius. GH is secreted and synthesized from somatotroph cells of the anterior pituitary lobe and plays significant role in...
Yue Ren1, Peng-Fei Liu2, Wan-Long Zhu1,*,Hao Zhang1 and Jin-Hong Cai1
...RMR), nonshivering thermogenesis (NST), food intake, serum leptin levels and hypothalamic neuropeptide Y (NPY), agouti aelated peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that body mass and serum leptin levels were lower significantly in winter than that of in summer in five areas. But thermogenic characteristics and food intake were higher significantly in winte...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
... expression levels of 12 genes, including fatty acid binding protein 4 (FABP4), acyl-CoA dehydrogenase short/branched chain (ACADSB), ELOVL fatty acid elongase 3 (ELOVL3) and solute carrier family 27 member 1 (SLC27A1), were altered upon ABCA1 down-regulation using RNA interference (RNAi). Overall, ABCA1 expression influenced triglyceride metabolism in BMECs by regulating the expression of related genes in the lipid metaboli...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Muhammad Imran Khan1, Muhammad Afzal1, Azhar Abbas Khan2, Syed Muhammad Ali Zahid1, Muhammad Saqib1, Asad Abdullah1, Saba Kousar1 and Maryam Riaz1
...illus thuringiensis transgenes (CIM-602 and CIM-599) and one non-transgenic genotype (CIM-554) for the performance of H. armigera feeding The difference in leaf injury, relative consumption and growth rate of H. armigera was detected among experimental factors and their interactions. The leaf injury caused by H. armigera was higher (38.5 cm2 and 30.5cm2) on non-transgenic genotype at high and low moisture levels of plants respectively. Similarly, the relative ...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
... considered as candidate genes for growth because they play a significant role in development and growth regulation. IGF-1 control protein metabolism and is extremely conserved region amongst species. IGF-1 have not been studied before in camel. The DNA samples of Marecha camel were collected from the Camel Breeding and Research Station at Rakhmani Bhakkar, Pakistan. Four polymorphic sites were detected in the IGF-1 gene. A significant finding was the o...
Memis Ozdemir
...pression of milk protein genes. It has been seen many studies about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in <...
Riaz Hussain Pasha1*, Muhammad Zubair Anjum2, Imran Ullah2, Muhammad Akram Khan3, Adnan Ali4, Saif-Ur-Rehman5, Sana Batool2 and Arslan Emmanuel2
...uginea and rapid spermatogenesis in testes while appearance of cortical alveoli or yolk vesicle in cortex of cytoplasm in ovaries. Gonads attain maximum size and weight in spawning phase during summer. Free oozing of spermatozoa in testes and ovaries packed with fully grown eggs are the distinct features of this phase. The highest values of Gonadosomatic Index (GSI) for male and female in summer coincided with the histological structure of the gonads where the...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...ction life. Precise pathogenesis job rheumatoid factor not known, through formation immune complexes engross establishment harmonize. To determine the correlation between Anti-Oxidants and Biochemical status in Rheumatoid Arthritis patients. 60 clinically diagnosed Rheumatoid Arthritis (RA) patients and 50 healthy persons were included in this study. 5 ml Blood was drawn and serum was separated. Anti-Oxidant Biomarkers, Serum micronutrients, serum Electrolyte ...
Hafiza Sadaf Zahra1, Asia Iqbal2, Sayyeda Hira Hassan1, Hafiz Abdullah Shakir1*, Muhammad Khan1*, Muhammad Irfan3, Chaman Ara1, Shaukat Ali4
...tion of tumor suppressor genes (TSG), while in hypomethylation, demethyltransferase enzyme causes the activation of oncogenes by promoting transcriptional activity. Contrary to DNA methylation, histone acetylation and deacetylation results in chromatin opening and closing, respectively; leading to transcriptional activation and inactivation of genes. Histone acetylation has been frequently...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...ping VP1-VP2 and VP2-VP3 genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of currently circulating strains of CAV; however, they require further analysis to intervene in necessary strategies to avoid future outbreaks in commercial poult...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...xious effects on spermatogenesis in mammals. Forty mice, randomly categorized into four groups (N=10) were administered orally with different concentrations of levofloxacin (0.00, 9.37, 18.75 and 37.50µg/g BW of mice) for 30 days consecutively. Mice were sacrificed on 31st day (24 hours after last dose administered) under deep chloroform anaesthesia. Blood was extracted through cardiac puncture and viscera were dissected out for further analys...
Peng Chen1,2, Zuhao Huang3,Chaoying Zhu1, Yuqing Han1, Zhifeng Xu1,
Guanglong Sun1, Zhen Zhang1, Dongqin Zhao4, Gang Ge1 and Luzhang Ruan1*
...codons in protein-coding genes (PCGs) included ATG, GTG, ATT, ATC and ATA, while its stop codons included TAA, TAG, AGG, AGA and the incomplete cipher T. In PCGs, the highest frequency of codon was CTA (Leu). The highest frequency of amino acids was Leu, whereas the lowest was Cys. In phylogenetic analyses, Gruiformes included Grui and Ralli, and Charadriformes included Charadrii, Lari and Scolopaci. The genus Porzana was closest to Porphyrio.
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
...ondrial ATPase8/6 genes in Sindh ibex and to determin its phylogenetic relationship with other capra species. Blood samples were collected from Kirthar National Park, Sindh, Pakistan. PCR product was sequenced bi-directionally using dideoxy chain termination method after mitochondrial ATPase8/6 genes amplification. Polymorphism, Genetic diversity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP a...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...y strains like; Alcaligenes spp. N14 and N21, Bordetella spp. N30 and Streptomyces spp. N28. Alcaligenes spp. N27 and Lysinibacillus spp. P17 showed strong activity against all the three ATCC strains. The study concludes that the bacteria isolated from the rocks having substantial resistance to heavy metals are also showing good antibacterial activity as well as, and they are also potential...
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslam3 and Samina Jam Nazeer Ahmad1, 2
...ethrin). Cytochrome P450 genes have been associated with insecticides resistance. Field collected population of R. ferruginous from different provinces of Pakistan was investigated for insecticides resistance development against P450 gene. Insect bioassay indicated highly significant level of LC50 values among laboratory reared and field collected population against Cypermethrin. The laboratory reared resistant population, Sindh and South Punjab collect...
Huanxin Zhang1,2, Hongshuo Tang3, Jun Chen1, Yu Zang1,2, Xuexi Tang1,2,* and Ying Wang1,2,*
... African hunting dog TLR genes are scared. The available genome sequence of African hunting dog offers us the way to examine the innate immunity of this endangered carnivore. 10 TLR genes (TLR1-10) were initially identified from the African hunting dog and lesser panda. The results showed that most of the TLR genes were very conservative in the African hunting dog. We found 6 of ten TLR
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*
...tor pathways in the pathogenesis of Non-Hodgkin Lymphoma (NHL). Binding of growth factors/ mitogen/cytokine or interleukin to EGFR leads to Ras induced RAF-MAPK cascade activation. PTEN protein is involved in the negative regulation of PI3K pathway. Mutations in upstream kinases, growth factor receptors or intrinsic members of cascades can lead to induce or promote cancers. Number of somatic mutations in several genes, major...
Abdul Samad1, Raheela Asmat2, Muhammad Naeem1, Hamida Ali3, Mohammad Zahid Mustafa1, Ferhat Abbas1, Jannat Raza1,3 and Muhammad Tauseef Asmat1*
... for Listeria monocytogenes and its subsequent transmission to food chain, plant material and animals. In present study, 800 food samples consisting of beef and chicken meat, milk, salads and fresh vegetables were collected from retail markets of Quetta, Pakistan. Multiplex PCR was employed to identify the prevalent species and genes associated with virulence. Only 10 (1.25%) samples were found contaminated with Li...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...ite different color MC1R genes of Nubian goats revealed that there are three SNPs in the gene sequence, which are 219, 712 and 1160, respectively. The C/T mutation did not cause amino acid mutations; base A/G mutations occurred at position 712 and caused amino acid mutations in pure black and brown goat samples, which were mutated from tryptophan to cysteine. The results of this study have important reference significance for the future correlation analysis be...
Anand Kushwaha, Amit Kumar, Aparna Madhavan, Durga Goswami, Golmei Poulinlu and Gnanavel Venkatesan*
...al immunogenic candidate genes. Selection and use of such candidate immunogenic proteins from an array of genes located at different structures of a mature virion are the real challenge and time consuming task for researchers. Nevertheless, identification of candidate gene(s) using advanced bioinformatic tools will ease the process and can select the suitable protein(s) to use in the development of specific and sensitive dia...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...p A and Ceu E genes, respectively. The antibiotic resistance of the Campylobacter isolates was determined via the disc diffusion method and was observed most frequently to nalidixic acid (81.8 %), tetracycline (72.7 %), ciprofloxacin (54.5 %), and erythromycin (54.5 %), while low resistance to ceftriaxone (18.2 %) was detected. Among the 11 Campylobacter isolates, 9 isolates were multidrug resistant (MDR). The tet (O) gene, whic...
Mohammad Aquil Siddiqui1*, Muhammad Tahir Khan1, Ghulam Shah Nizamani1, Shafquat Yasmeen1, Imtiaz Ahmed Khan1, Abdullah Khatri1 and Nighat Seema Soomro2
Muhammad Ilyas1, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Waqar Ahmed1, Muhammad Riaz Khan2, Raja Mohib Muazzam Naz2, Muhammad Mohib Ullah Khan3 and Sumaira Hafeez1*
...l (mdhij) indicated that genes which control the grain yield were mostly dominate in nature but also influenced by the epistatic effects of additive genes under natural conditions. These results favoured that the hybrid breeding is the best option in the given set of inbreds to best exploit their potential. ...
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
...dy was to find candidate genes regulating the growth and development of sheep. The polymorphism of GHSR gene in five sheep populations was analyzed by PCR and sequencing techniques. The results showed that large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep, hu sheep all have two mutation sites (C155T and C624T). For locus C155T, allele frequencies of C in large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep...
Jun Yan Bai1*, Xiao Ping Jia2, Xiao Hong Wu1, Guang Lu Li1, Heng Cao1, Xue Yan Fu1 and Kun Peng Shi1
...dy, polymorphism of FMO3 genes in Beijing white quail, China yellow quail and Korean quail was tested by PCR technology and hybrid DNA pool sequencing technology. Results can provide references for further exploring the action mechanism of FMO3 on fishlike smell of quail eggs. Research results show that, One SNPs mutation site (A66G) was detected from the exon 2 of FMO3, the A allele frequencies of A66G in Beijing white quail, China yellow quail and Korean qua...
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
...>Aspergillus minisclerotigenes and Aspergillus flavus are morphologically similar species that belong to the Aspergillus section Flavi. A. minisclerotigenes and A. flavus were isolated from soybean and okra seeds, respectively. The isolated species were first identified morphologically. ITS1–5.8S–ITS4 primers sequence and amplification of ISSR nucleotide sequences using thre...
Mehtap Bayir*
...sod1 and sod2 genes in fugu—a model organism—were determined. Maximum amino acid similarity identity was found between putative fugu Sod1 and Sod2 proteins and their orthologs from teleost fish and tetrapods. Phylogenetic clustering was seen between sod genes in fugu and their orthologs. Finally, highly conserved gene synteny was determined between fugu sod genes<...
Yunfang Tian, Zeqin Fu, Jiantong Feng, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...>COI and Cytb genes). The genetic diversity of seven samples was in a high level (h = 0.879-0.949, π =0.0033-0.0110). The Bayesian skyline plots showed that the samples were a stable population. The genetic divergence of seven samples was suggested that there was detected two obviously groups from pairwise FST values and networks. This differentiation was conformed to the geographic of the sampling sites. However, ...
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...ty through mitochondrial genes in saw-scaled viper from Pakistan. Tail tip biopsies of Saw-scaled vipers were used for amplification of mitochondrial genes fragments (ND4, 16S rRNA and 12S rRNA) through Polymerase Chain Reaction (PCR). Nucleotide data was used for DNA polymorphism analyses and homology was measured among different species of genus Echis. Using the concatenated nucleotide data, Maximum likelihood and Bayesian...
Shilong Chen1,2, Shifeng Xiao1,2, Shao Wang1,2, Fengqiang Lin1,2, Xiaoxia Cheng1,2, Xiaoli Zhu1,2, Dandan Jiang1,2, Shaoying Chen1,2* and Fusong Yu3*
...ic organization and pathogenesis. However, the pathogenesis mechanisms of cross-species transmission, retardation and beak atrophy are still needed to be elucidated. ...
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
...on of antioxidant enzyme genes including nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), SOD, and glutathione peroxidase (GPx) were improved by FR supplemented into the broiler diets. Based on these results, FR could be acted as a beneficial feed additive with antioxidant capacity and meat quality-improving effect in broiler diets.
...
Aftab Shaukat1,*, Sana Hanif2, Irfan Shaukat3, Muhammad Ahsan Naeem4,5, Shadab Shaukat6, Rizwan Shukat8, Shahid Ali Rajput9, Imran Shaukat10, Mubashar Hassan4, Khalid Mehmood7, Saqib Umar11 and Muhammad Ali Jamil1
...silencing of one or more genes. Despite their importance as gene regulator, computational strategies are still at its initial stages. Several computer based prediction databases remain the only foundation for rapid identification of putative microRNA target. By utilizing experimentally validated targets, the search of further targets via bioinformatics tools help to predict gene target site. Keeping this in view, a study has been planned to investigate PDCD1 g...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1
...yt-b and Cox1 genes. A total of 749bp of Cox1 and 472 bp of Cyt-b complete coding regions of both genes were amplified by PCR followed by sequencing. The sequences were aligned and edited using Bio-Edit software and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA7 explained the genetic relationships of A.chukar with related ...
Expression Analysis of BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 in Rams with Different Fecundity
Weihao Chen1,2, Zhilong Tian2, Lin Ma2, Shangquan Gan3, Wei Sun1,4,* and Mingxing Chu2,*
...d5, and Smad9 genes in rams with different fecundity, quantitative real-time polymerase chain reaction was used to investigate the expression level of six genes in the brain, cerebellum, hypothalamus, pituitary, testis, epididymis, vas deferens, and adrenal gland in high fecundity (Small Tail Han sheep) and low fecundity (Sunite sheep) rams. The results were as follows: BMPR1B, GDF9, Smad1,
Asmaa Abdallah Darwish
...rent ovine diseases pathogenesis and complications and their supplement for healthy and diseased animals is strongly recommended. ...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
...database to identify key genes in aspirin-damaged yeast cells following redox injury. Differentially expressed genes (DEGs) were subsequently identified and functionally enriched for analysis. Additionally, a protein-protein interaction network (PPI) was constructed and block analysis was performed using STRING and Cytoscape databases. A total of 248 genes were identified, of which 84 were...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...ing was performed and unigenes were identified after assembly and clustering. The resulting unigenes were then compared to homologous sequences using NCBI-NR, Swiss-Prot, KOG, Pfam, eggNOG, KEGG, COG, and GO for functional annotation and classification. Some differences in the regularity of distribution and the time of eclosion of the pupa were detected between the southern and the northern populations. We obtained 142.65 Gb...
Yue Qin Song, Hui Zhong Sun* and Jun Feng Dong
...s olfactory receptor genes (YsigORs), including one olfactory co-receptor (Orco), were identified in this study. All the sequences were further validated by cloning and sequencing. Tissue expression profiles of all YsigOR genes in the antennae of females and males were analyzed using real-time quantitative PCR (RT-qPCR). The result showed that some YsigOR genes displayed significant di...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...ques. Virulence and pathogenesis of the disease were determined by intraperitoneal injection of the etiological agent to the healthy A. sinensis. Physiological and 16s rRNA molecular analysis identified Streptococcus iniae as the causative agent of the disease outbreak. The bacteria has a unique biochemical profile compared with most of the previously isolated strains. Antimicrobial susceptibility tests revealed that S. iniae HNM-1 is...
Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5*
...he rotavirus VP7 and VP4 genes were amplified. The DNA was purified, sequenced and genotypes were determined using the RotaC online classification tool. A phylogenetic tree was constructed using the Maximum Likelihood method applying the Tamura 3-parameter model in MEGA 6.06 package and statistically supported by bootstrapping with 1000 replicates. The amino acid sequences of the antigenic regions of the outer capsid proteins VP8* were compared with vaccine an...
Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole
...e relevant to HIV-1 pathogenesis and disease. HIV-1 genome has nine genes; gag, pol, and env genes are common in all replication-competent retroviruses, and the pol gene is very unique encoding the enzymes for replication. Our objective is to describe the relatedness of these HIV-1 pol genes with each other and its antiviral drug susceptibility which wil...
Hafiz Muhammad Imran Javed* and Mushtaq Ahmed
Fang Liu*
...endent functions in angiogenesis and hypothalamic axon guidance. At lower concentration of 100ng/ml, FGF10 promotes angiogenesis in chorioallantoic membrane endothelial capillaries (CAMs), but FGF10 does not alter the endothelial growth significantly at higher concentration (500ng/ml, P>0.05). Simultaneously, FGF10 exerts a graded action, at lower concentration FGF10 attracts both magnocellular and parvocellular axons; bu...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
...r the regulation of adipogenesis. However, the differentially-expressed microRNAs (miRNAs) involved in SMSC adipogenesis induction, and the role of these miRNAs in Wnt/β-catenin signaling, has received little attention, particularly in livestock. In the present study, bovine skeletal muscle satellite cells (bSMSCs) were cultured and the Wnt/β-catenin signaling pathway was activated by LiCl. Investigation of the pat...
Muhammad Khan1, Tehmina Ameer Khan1, Aziz Ud Din2, Muhammad Fiaz Khan1, Irfan Ullah3,*, Kalim Ullah4, Sadia Tabassum1,*
...ee mitochondrial encoded genes 16S-rRNA (MT-RNR2), tRNAIle (MT-TI) and tRNALeu (MT-TL1) were targeted for analysis of mutation. Genomic DNA was extracted from buccal swab using Phenol: Chloroform: protocol. The target genes were amplified via polymerase chain reaction (PCR). The amplified gene products were sequenced and compared with the revised Cambridge Reference Sequence (rCRS) Accession-No. ...
Zengwen Huang1,2, WuReliHazi Hazihan2*, Baheti Bodai2, Kadyken Rizabek3, Nuralieva Ulzhan3, Omarova Karlygash4, Juan Zhang1 and Yaling Gu1*
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...16S rRNA and merA genes and a specific restriction patterns was successfully obtained after treatment with different endonucleases. A small scale reservoir of Luria Bertani (LB) medium supplemented with 30 μg/mL of HgCl2 was designed to check the detoxification ability of the selected strains. The results demonstrated 83% detoxification of mercury by both B. cereus AZ-2 and B. cereus AZ-3, and 76% detoxification by Bacillu...
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
... antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX (27%), CIP (25%), LFX (13%) and AZM (11%) while the least resistance was found a...
MA Liman1, QI Yongxiao1, Wang Wenji2* and Zhong Qianyi3*
..."font-size: small;">Angiogenesis, the formation of new blood vessels, is tightly regulated in the normal organism. Excessive angiogenesis can have detrimental consequences in various processes, including promoting uncontrolled tumor growth. Inhibiting angiogenesis using natural compounds is an important focus in developing new cancer treatment strategies. Ginsenoside Rh2 (G-Rh2), a natural...
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
...xplants sources for callogenesis and regeneration in maize (Pioner 3025). The main explants under consideration were: three parts of germinated seedlings, mature embryo and split seeds. Each of these explants was evaluated for its ability to form callus and to regenerate subsequently. For callogenesis from different parts of germinated seedlings, maximum frequency was shown by bulged inter node (45.60 %) along with the regen...
Hong Hu1, 2, Li Qian1, Yuanlang Wang1, Chaodong Wu1, Xiaodong Zhang1, Yueyun Ding 1*, Li Wang1, Xudong Wu1, Wei Zhang1, Dengtao Li1, Jian Ding1, Min Yang1 and Zongjun Yin1*
...mechanisms and candidate genes associated with fat metabolism in Anqingliubai (obese) and Yorkshire (lean) pigs. The transcriptome profiling of backfat between Anqingliubai and Yorkshire pigs was carried out by RNA-sequencing technology. The sum of clean reads were 288.3 and 365.3 million which was obtained from the RNA sequencing data in the Anqingliubai and Yorkshire pigs, respectively. Most reads were located in exonic region, while less reads were located ...
Fan Sigang1, Guo Yihui1* and Xu Youhou2
...ly. Reproduction-related genes, including vasa, nanos, and vitellogenin, and sex-determining genes, such as FoxL2, Dmrt, and sox9, were detected. Transcriptome comparison revealed 2,842 differentially expressed genes (DEGs), of which 591 exhibited biased expression in the ovary and 2,251 exhibited biased expression in the testis. Gene Ontology (GO) and Kyoto Encyclopedia of
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
...s is due to complex pathogenesis comprising a network of signaling pathways. By controlling one pathway, the pathogenesis continues through complementary pathways. Janus kinase and bruton’s tyrosine kinase regulates a number of T-cell and B-cell mediated signaling pathways ranging from cytokine expression and antibody production to the bone resorption and cartilage destruction. We propose a dual trap to control this pa...
Comparative Analysis of Muscle Development in Zebrafish with Different Intermuscular-Bo nes Patterns
Jian Yang1, Guangxiang Tong2, Zhipeng Sun2, Xianhu Zheng2, Weihua Lv2, Dingchen Cao2, Xiaowen Sun2 and Youyi Kuang2*
...ssion of muscle-specific genes and muscle structure. Five muscle-specific genes including myod, myog, myf5, mef2ca and sox6 were selected to test and verify the expression differences in embryonic development stages (3 hpf, 6 hpf, 12 hpf, 24 hpf, and 72 hpf) and post-embryonic stages (15 dpf, 30 dpf, 45 dpf, 60 dpf and 75 dpf). Compared to the wild-type (WT) siblings, the mutants showed no ...
Faiqah Ramzan1,2,*, Irfan Zia Qureshi2 and Muhammad Haris Ramzan3
...rone release and spermatogenesis. Intraperitoneal kisspeptin administration was carried out in 05 weeks old prepubertal male Sprague Dawley rats, twice daily for 12 days, at three different dosage regimens: 10ρg, 1ηg and 1µg compared to control (saline-treated) rats. Effects on pituitary gonadotropins [Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH)], secretion of testosterone and spermatogenesi...
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
... bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazo...
Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo
The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...gnita was utilized. Many genes expressed in nematode feeding cells or the regulatory regions that control these genes have been isolated. Transproteins toxic to different plant parasitic nematodes can be expressed into tissues and cells feed upon by nematodes. Transgenic products with a potential to interefere with nematode physiology such as digestive enzymes or structural proteins of the intestine are considered....
Inga Kowalewska-Łuczak1 and Ewa Czerniawska-Piątkowska2,*
...="text-align: justify;"> genes on selected milk and reproductive traits in the Polish Holstein-Friesian cattle (black and white variety). Analysis of genotypes of individual species was carried out using the PCR-RFLP. The frequency of the most occurring alleles for the individual polymorphisms studied was the following g.18174 A>G NCF4 gene
Yifan Ni1, Jian Liu1, Fen Wu1, Jianfeng Cai1, Jinzhi Zhang1*, Jianqing Hua2 and Jiping Fu2
... SNP loci found in these genes totally. One mutation (G→A) was detected in exon 1 of MDR1 gene, which caused a synonymous mutation of amino acid (Leu→Leu), the other one (T→A) in exon 7 of OPN gene, resulting in a non-synonymous mutation (His→Gln). The association analysis indicated that the GG genotype of MDR1 gene and the TT genotype of OPN gene had the highest values for the total number of piglets born (TNB),...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1, Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...tential role in the pathogenesis of rheumatoid arthritis (RA). Due to the confounding results, reported in several studies, we performed the systematic literature search on IL-6 -572 G/C polymorphism (rs1800796 SNP) and assessed its association with RA in the overall world population, and in the subgroup analysis of Asian and Caucasian populations. The results showed that in overall population, IL-6 -572 G/C shows a significant association towards increased ri...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
...for cellular de novo lipogenesis. THRSP gene encodes a nuclear protein which regulates fatty acid synthesis in lipogenic tissues. Identification of single nucleotide polymorphisms (SNPs) of sheep THRSP gene and their association with fat deposition were investigated using Altay and White Suffolk sheep. In addition, the messenger RNA expression profiling of THRSP in fat-tailed and thin-tailed breeds was compared among 8 different tissues. F...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
... been involved in carcinogenesis. So far, little is known on porcine E2F3. Here, we cloned the complete coding sequence (CDS) of E2F3b and identified seven alternatively spliced transcripts in pigs using molecular biology technique for the first time. The CDS of the canonical transcripts (named V1) of porcine E2F3b is 1023 bp in length, and showed 93.35% and 90.62% identities with the homologues from human and mouse, respectively. The splicing variants were pr...
Zhen-Yang Wu1, Li Li1, Yu-Hua Fu2, Sheng Wang3, Qing-Ming An1, Xiao-Hui Tang4, Xiao-Yong Du3,5,* and Fei Zhou6,*
... the functional roles of genes in Tibetan sheep skin with different coat colors, we sequenced genes from six skin samples using Solexa sequencing. The RNA-Seq analysis generated 63,283,784 and 63,644,062 clean reads in black and white skin, respectively. A total of 60 differentially expressed genes (DEGs) were identified, providing evidence that the different coat color skin changed consid...
Sabina Noor1*, Fatimah Abang1 and Hamady Dieng2
Biology of an Exotic Butterfly Acraea terpsicore (Linnaeus, 1758) (Nymphalidae: Heliconiinae), in a Newly Invaded Region, Sarawak, Borneo
... larval instars. The ontogenesis of larvae was accomplished without any disruption of diapause. The female adults were ochreous orange in color with a wingspan of about 50-60 mm. The males were brighter orange in color and had a wingspan of about 40-50 mm. The population index was found to be common throughout the year but declined during months with higher precipitation. Being an exotic species, A. terpsicore is likely to keep track by feeding on its primary ...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...ficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
...c synergy test. The ESBL genes blaCTX-M, blaOXA-10, blaPER-1, blaSHV and blaTEM were analyzed by PCR amplification among the isolates. Susceptibility to antibiotics was: imipenem (85.02%), meropenem (82.88%), cefepime (76.47%), piperacillin-tazobactam (76.47%), colistin (74.86%), ciprofloxacin (74.33%), piperacillin (72.19%), ceftazidime (68.98%), ofloxacin (68.44%), amikacin (66.84%), cefoperazone (66.31%), carbenicillin (66.31%), gentamicin (64.7%), tobramyc...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...le approach coupling mutagenesis and growth & second metabolites were used to isolate strains with increased stress resistance and virulence. Increased virulence in some of the mutants correlated with increased insecticidal activity in cell-free extracts that could potentially be used directly for insect control.
...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...function such as steroidogenesis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
...i>S. Gallinarum pathogenesis in vivo.
...
Uwem E. George1, Oluwadamilola A. Arowolo2, Oluseyi A. Olayinka3, Ijeoma M. Ifeorah4, Temitope O.C. Faleye3,5, Bolaji Oluremi6, Arthur O. Oragwa7, Ewean C. Omoruyi8, Emmanuel E. Udoh9, Oluwadamilola G. Osasona1,10, Emmanuel Donbraye11, Olufunke P. Adeniji12, Olubusuyi M. Adewumi3,13* and Johnson A. Adeniji3,13,14
...ycline resistance (TC-r) genes, while remaining 9.5% (2/14) inhibit cell wall synthesis (cfxA3_1 and cfxA6_1). Eight (aph(3’)-III_1, cfxA3_1, cfxA6_1, erm(B)_10, tet(Q)_1, tet(Q)_2, tet(Q)_4, tet(W)_5) of the ARGs detected were predicted to be plasmid-borne. We report using a cultivation-independent approach the presence of ARGs in Nigerian Chimpanzees. Findings suggest Nigerian Chimpanzees may constitute a hitherto overlooked source of antibiotic resist...
Chaogang Yao, Daxin Pang, Chao Lu, Aishi Xu, Peixuan Huang, Hongsheng Ouyang* and Hao Yu*
...ion profile of candidate genes involved in the PPAR and fatty acid metabolism signaling pathways were detected in the LD muscle of two pig breeds with different IMF contents (Large White and Min) by a quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) array. Our results showed that three lipid metabolism-related biological processes lipogenesis, fatty acid transport and fatty acid oxidation in t...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
...f prostaglandins-related genes in H9N2 infected chicken’s erythrocytes have not been studied yet. So, the present study was designed to investigate the PG-related genes in erythrocytes of H9N2 infected chickens. In current study, qRT-PCR and Western blot analysis were performed to identify the expression levels of PG-related genes (COX-2, GSTA3, PTGDS, PTGES, PTGER3 and PTGER4) in er...
Oluseyi A. Olayinka1, Temitope O.C. Faleye2, Oladipo O. Omotosho3, Oladapo A. Odukaye3, Bolaji Oluremi4, Ibipeju H. Ibitoye1, Oludayo O. Ope-Ewe1, Uwem E. George5, Oluwadamilola A. Arowolo6, Ijeoma M. Ifeorah7, Ewean C. Omoruyi8, Emmanuel Donbraye9, Olufunke P. Adeniji10, Olubusuyi M. Adewumi1,11* and Johnson A. Adeniji1,11,12
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...ed bacteria’s pathogenesis, but still, the literature about the role of bacteria and its immunogens on the demonstration of clinical signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein ...
Sara Sultan Alomran1, Muhammad Nasir Khan Khattak1,2, Amir Ali Khan1,2*, Sallam Hasan Abdallah2, Abeer Maher Fayyad1 and Khalid Bajou1,2
... to target more than 500 genes, MAPK has not been identified as one of the more than 500 gene targets of miR-22-3p, though this pathway was upregulated in transfected iMSC3 that differentiated to adipocytes. Finally, miR-22-3p was observed to suppress adipocytes and was involved in adipocyte differentiation through non-targeted MAPK pathway upregulation rather than AKT3-related processes.
...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
...identity with IFNα genes of other species, including C. ferus, Vicugna pacos, and Homo sapiens. Expression of C. dromedarius IFNα cDNA in Escherichia coli revealed a fusion protein with a weight of 22.5 kDa after induction of expression with IPTG for 5 h. The recombinant IFNα was expressed in the form of inclusion bodies that were separated and solubilized in vitro and the protein was refolded using S...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2,
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
Jabeen Farheen1,2*, Simeen Mansoor1 and Maria Abid1
... humans’ proto-oncogenes which leads to carcinogenicity. The current findings aimed to evaluate the genotoxic impact of widely used azo FCAs on the cell cycle by using onion as a model plant. The study was designed in a complete randomized design where the grown onion roots were exposed to 0%, 0.001%, 0.01%, 0.1%, and 1% concentration of FCA for 120 hours for macroscopic and 36 h for microscopic evaluation. The onion root tips morpho-physiology was sever...
Fayaz Ahmad, Noorullah Khan, Farrukh Siyar Hamid, Abdul Waheed, M. Abbas Khan, Imtiaz Ahmad, Shamsul Islam, Basharat Hussain Shah, Sohail Aslam and Qamar uz Zaman
...the presence of additive genes in the trait and suggested reliable tea crop improvement through selection of the traits. Significant and positive correlation was observed among all the traits, indicating that these traits are genetically interlinked and which may facilitate indirect selection of one trait for the other trait that is genetically interlinked....
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
... Anemia pathway mediated genes for activating cellular DNA repair pathway alleviated DNA damage at high LPS levels.
...
Shuang Yang1,2, Huiting Zhao3, Xuewen Zhang2, Kai Xu1, Lina Guo1, Yali Du1 and Yusuo Jiang1,*
...patterns of chemosensory genes and better understand the underlying olfactory mechanism. In the results, a total of 121 chemosensory gene transcripts were identified, including 37 odorant-binding proteins, 35 chemosensory proteins, 33 olfactory receptors, 14 ionotropic receptors and 2 sensory neuron membrane proteins. The expression patterns of these genes were determined using the estimated fragments per kilobase of transcr...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...mental arrest, chitinase genes and genes that are vital for starting the differentiation program of cells at discrete steps during development are major targets for biocide plant extracts. The current study evaluates the in vitro toxic effects of golden piller (Cupressus macrocarpa) and galangal (Alpinia officinarum) against Spodoptera frugiperda Sf9 cell line. Also, changes in gene expression levels of ...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
... carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine samples, respectively. The PCR results revealed that71.2% of blaCTX-M, 67.5% blaCTX-M -1 and 62.2% blaTEM producing isolates were recovered from poultry, 19.1% blaCTX-M, 2...
Zeynep Sonmez1 and Memis Ozdemir2,*
...tified in the determined genes and gene regions as a result of the sequence analysis. In the 1st exon of the EPS8 gene, 9 polymorphic regions were identified as g.94554132C>T, g.94554252G>C, g.94554348T>G, g.94554354C>G, g.94554372C>G, g.94554389A>G, 94554392C>G, 94554399A>G, and 94554439C>G. Within the region examined in the 2nd exon of the EPS8 gene, only the g.94555920T>G polymorphism was ide...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
... while the Bcl-2 and BAX genes regulate apoptosis. This experiment adopted the real-time fluorescence quantitative technique to test ZFX, Bcl-2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after birth. The results showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. The ZFX gene may participate in the developmental process ...
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...er laccase (LAC1) genes among the identified virulence factors. While capsular associated protein (CAP59) gene identified alone or associated with LAC1 gene in the other 2 C. gattii isolates. This study underlines a potential association of those fungi with human disease in Egypt. In order to strengthen existing therapeutic and control approaches, further analyses of the main risk factors and the other virulence of these pathogens s...
Maha I. El Zaafarany1, WafaaM. Badawy2, Eman M. El Salakh3
...related to breast carcinogenesis remain poorly understood. Some reports have examined the role of Human Papillomavirus (HPV) in this disease. The purpose of this study was to determine the prevalence of HPV in breast cancer.Fifty four fresh frozen breast cancers samples were analyzed. Samples were tested for HPV by PCR, and products were sequenced. Findings were correlated with clinical and pathological characteristics.The HPV DNA prevalence in the breast canc...
Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.
...s possible role in patho-genesis of HCC. The study included sixty individuals; they were classified into three groups; 20 patients with hepatitis C virus infection, 20 patients with hepatitis C virus-related HCC and 20 unrelated healthy volunteers who served as controls. Qualitative determination of Hepatitis C Virus antibody and quantitative determination of RNA-HCV by RT-PCR were done. MnSOD activity was evaluated using a superoxide dis-mutase assay kit. Gen...
Amal Mahmoud1&2 and Medhat H. Hashem3
... (pol) and envelop (env) genesTwo HBV isolates were associated with HCC (Sohag1 and 7). Phylogenetic analysis of the nucleotide sequence and the amino acid of the pol or env genes revealed that 7 out of the 8 isolates were closely related to genotype D. Only Sohag 1-HCC isolate was clustered with genotype C which is known as risk factor of HCC. Multiple sequence alignment resulted in unique amino acid substitutions (V320&rar...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...eotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–100%) identity with Egypt 2012/ and Palestinian-Gaza virus. Phylogenetic analysis of serotype O of this study from Ismailia (KJ210076) , Alexandria (KJ210074) and El-Mania (KJ210077) showed 100% identity with each other and 92% identities with Asian type O sequences from P...
Aly M. Abdel-Salam
... to amplify coat protein genes for the two viruses using specific primers. DAS-ELISA and immuno-blotting were used for evaluating the induced antisera. Results: Antisera for CYSDV and CVYV were produced efficiently and used for virus diagnosis through DAS-ELISA, DBIA, and TBIA. RT-PCR confirmed the nature of the two viruses. However mixed infection was noticed for CVYV and CVYV upon using duplex RT-PCR. Conclusion: Mixed infection with WTV is common and compli...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
... reading frame (ORF) 103 genes. The sequences
designated Sheep pox virus was submitted to GenBank under accession number of MF443334.
Conclusion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the
characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology of SPV in El Menofiya governora...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...wo major surface protein genes (MSP1a and MSP4) sequence were compared with cattle isolates from different origins. Phylogenetic analysis of local isolates showed a close homology with the isolates from Australia, Brazil, Turkey and Japan. It was found that aged, exotic and crossbreed cattle were more susceptible to A. marginale infection in summer season compared to the younger and indigenous cattle breeds, respectively (P
Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1
...iv>
levels of pathogenesis related genes, chitinase, PAL1 and LOX1.
Conclusion: In cucumber, treatments with artificially generated wind and root colonizing F. equiseti
GF19-1 or its CF elicited induced resistance against CMV infection, leading to a restriction of
pathogen propagation and disease development.
...
Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2
...ofile related to defense genes was evaluated at 1, 2 and 3 weeks after BYMV inoculation by quantitative real-time PCR analysis.
Results: Treatments with PGPF resulted in lower virus severity and titer than the control. GP17-2 treatment exhibited the lowest disease severity and virus titer in comparison with other treatments. Quantitative real-time PCR results showed significant increased expressions of pathogenes...
Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1
...ession levels of defense genes were assessed by reverse transcription PCR (RT-PCR) analysis.
Results: Both of virus severity and titer were significantly reduced in treated plants in comparison with the control. All growth characters and yield of cucumber plants were increased in GU23-3 treated plants than the control. RT-PCR analysis revealed increased expressions of different pathogenesis related
Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski
...e presence of resistance genes (CmeB, Blaoxa-61, tet(O), aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicrobial resistance of the C. jejuni isolates towards three important antimicrobials (ciprofloxacin, erythromycin and tetracycline). The PCR method used revealed highest prevalence for Blaoxa-61 (25%), followed by CmeB and tet(O) genes (19.4%) and aa...
Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*
...052 bp) contained the 37 genes and a single control region (CR) typically found in neobatrachian mtDNAs. In the new mt genome, the ND5 gene and a TLPF tRNA cluster (tRNAThr, tRNALeu(CUN), tRNAPro and tRNAPhe) were located between the CR and the 12S rRNA gene. R. dennysi mitochondrial gene rearrangements observed here could be explained by the Tandem Duplication and Random Loss (TDRL) model. We us...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...ormal process of spermatogenesis and quality of semen. Moreover, metals can lead to decreased or no production of prostaglandin, seminal fluid and endocrinology of reproductive process and thus results in male infertility. It was also here attempted to characterize the drastic effects of heavy metals on male infertility, the hormone analysis and genetic determination of SRY gene as some of infertile human had smaller sized testis. Blood and semen sample...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1, Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...differentially expressed genes (DEGs) in these four pathways were upregulated significantly at 14 and 22 dpi, indicating that the immune responses of erythrocytes were induced by MDV. This study may help to elucidate the molecular mechanism of chicken erythrocytes relate to immune responses in MDV infection.
...
Jie Gao1, Guannan Wang1, Chuang Zhou1, Megan Price2, Jinnan Ma1, Xiaohong Sun1, Benping Chen3, Xiuyue Zhang2 and Bisong Yue1*
...2.7%), 13 protein-coding genes (PCGs), 22 tRNAs genes, 2 rRNA genes, a control region (CR) and a non-coding region (NC). We found strong support for F. cinereiceps being placed within Sylviidae (superfamily Sylvioidea)and for babblers being separated into two families, Sylviidae and Timaliidae, with four subfamilies within Timaliidae. This is one of many taxonomic arrangements for b...
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...nd fatty acid metabolism genes in goat mammary gland tissues and muscular tissues was aligned with GEO database, and tested by qRT-PCR. F1 transgenic mice were mated and then the target genes and protein expression level in F2 transgenic mice, as well as fat content in F1 and F2 transgenic mice muscle were examined. Successful transgenic goats generation was identified by testicula...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...d carbapenemase-encoding genes was performed. The bacteriological examination of the samples showed that 6 Salmonella strains [S. Typhimurium (3), S. enteritidis (2) and S. Infantis (1)] were isolated from chicken meat and eggshell surface samples. In human, it was found that 6 salmonella strains (S. Typhimurium, S. Enteritidis, S. Infantis, S. Virchow, S. Haifa, S. Kentucky) were isolated. The res...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
.../i>isregulated by >50 genes. Bioinformatics analysis of flagellar assembly in S. typhimurium identified several conserved structural elements. In this study, FliI a flagellar protein required for flagellar assembly and involved in a specialized protein export pathway was cloned and overexpressed. ΔfliI mutant Salmonella strain was used to transform fliI overproducing plasmid pTrc99A by electroporation. Using vital dyes (Ale...
Chaochao Hu1,2, Xue Xu1, Wenjia Yao1, Wei Liu3, Deyun Tai1, Wan Chen4 and Qing Chang1*
...indicated that all these genes evolved under purifying selection. Evidence of positive selection was obtained for three (ND2, ND4 and ND6) genes by at least one of the methods. This study provides a valuable resource facilitating further study of phylogenetic and evolutionary analysis for wader, and improves our understanding of the evolutionary and taxonomic research within Charadriiformes.
...
Zhengyi Fu1,2,3,4, Rui Yang1,2,3, Shengjie Zhou1,2,3, Zhenhua Ma1,2,3* and Gang Yu1,2,3*
... selected growth-related genes (GHRH, GH, GHR, IGF-I, IGF-II, MSTN-1 and MSTN-2), and immune-related genes (IL8, IL10, TGFβ1, TNF, INF-γ1, Mx, CRP, C3, C4, mTOR, mLST8, eIF4E, HSP70 and HSP90) to measure their relative expression level. Results from the present study indicate that the expression level of GHR, IGF-I and IGF-II is consistent with body length, but there is no similar rule between GHRH, GH, mstn-1 and...
Jia Li and Hong Yang*
...odegradation of BaA like genes quantity, the activity of enzymes and functional of bacteria, were also increased due to the APG and alkaline treatments. Overall, the instantaneous enhancement of microbial activity and BaA bioavailability increased biodegradation efficiency.
...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...o screen the armA genes among the A. baumannii isolates collected from clinical specimens at the tertiary care hospitals of Lahore and Faisalabad,Pakistan. A total of 148 A. baumannii isolates were collected. The initial identification of bacterial isolates was performed by standard microbiological techniques, and API 20E and for final confirmation multiplex PCR was performed using genus and species-specific primers. The susceptibility to ...
Usman Khalique*, Muhammad Zahid, Noor Fatima, Syed Jawad Ahmad Shah
...o identify the resistant genes in these varieties that could be incorporated in future wheat cultivars....
Anwar Khan1, Fareeha Nosheen2,4, Bushra Tabassum2*, Olawale Samuel Adeyinka2, Khurram Shehzad3, Naila Shahid2, Arif Muhammad Khan4 and Idrees Ahmad Nasir2
...al transmission and pathogenesis. RNA interference or post transcriptional gene silencing is a regulatory conserved mechanism that uses small interfering RNAs to control the expression of desired gene by inhibiting mRNA transcription. The present study aims to investigate the potential role of different siRNAs in down-regulating the mRNA expression of potato virus X in cell culture assays. Three specific siRNAs against CP-PVX gene were designed in which siRNA ...
Murat Onur Yazlık1, İnci Başak Müştak2*, Gültekin Ünal3 and Ufuk Kaya4
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1,
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...els of apoptosis-related genes in the H9N2 infected chicken erythrocytes. qRT-PCR and Western blot techniques were performed to determine the expression levels of apoptosis-related genes including Bax, Bcl-2 BAG-1, BAG-3 and STAT3 in H9N2 infected erythrocytes of chickens for incubation time intervals of 0, 2, 6 and 10 h. The expression of all the genes related to apoptosis were significan...
Mohammad Aquil Siddiqui1*, Muhammad Mahran Aslam1, Mahboob Ali Sial1, Nighat Seema Soomro1, Muhammad Tahir Khan1, Shafquat Yasmeen1, Shumaila Sial2 and Imtiaz Ahmad Khan1
... through the induced mutagenesis (gamma irradiations), released for general cultivation practices in the province of Sindh.
...
Jia Liu1, Zhen Wang1, Zifan Ning1, Ali Mujtaba Shah2,3,Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Yaofu Tian1, Diyan Li1,Gang Shu2,4, Lin Ye1 and Xiaoling Zhao1,*
...ion of several candidate genes that may modulate myofiber growth and thus explain some of the differences in the skeletal muscle phenotype of FG and SG chickens. We collected pectoralis major (PM) and gastrocnemius muscles (GM) on d 1, 7, 28, 49, and 70 post-hatch, and measured the weights of the muscles, diameter and, a density of myofibers, and the expression abundances of MyoD, MyoG, IGF-1, and Pax7. The body weight of FG was hea...
Song Jiang, Dong-liang Liu, Fa-lin Zhou, Xian-bin Mo, Qi-bin Yang, Jian-hua Huang, Li-shi Yang and Shi-gui Jiang*
...l;">In this study, three genes, PmATP synthase, PmNa+-K+-ATPase and PmToll receptor gene, were selected to study their relative expression in different tissues of Penaeus monodon. The results showed that the mRNA level of PmATP synthase gene was not significantly up-regulated under low salt stimulation (P> 0.05),while under high salinity stimulation, the transcription level of PmATP synthase...
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2, Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and
Carlos Alfonso Alvarez-Gonzalez4
... commonly used reference genes such as alpha elongation factor (ef1-α), beta-actin (actb), 18S ribosomal RNA (18s rrna), beta-2-microglobulin (b2m), tubulin alpha (α-tub) and glyceraldehyde 3-phosphate dehydrogenase (gapdh) in the different tissues of the intestine, muscle, gill, stomach, brain and liver in adult males of A. tropicus from reared in captivity through three BestKeeper, geNorm and NormF...
Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1
... in the RK-MTases genes; Ribosomal N-lysine methyltransferase1 (RKM1) and Ribosomal N-lysine methyltransferase4 (RKM4) in Sordaria fimicola using SNP markers. A total seven SNPs in the RKM1 gene and nine in RKM4 gene were identified. A subset of SNPs were unique in SFS strains and others were fixed in the NFS strains of S. fimicola. These polymorphisms might be adaptive in stressful environmental conditions. Gen...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
...fy polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and 0.27, respectively. The association of DRD2 polymorphism were detected on...
Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3*
.... Detection of virulence genes using pslA, toxA and exoU genes revealed that 29.4%, 23.5% and 17.6% respectively. Molecular detection of ESBLs encoding genes in P. aeruginosa recorded that blaTEM genes blaSHV and blaCTXM genes were detected in percentages of 64.7%, 47.0 % and 29.4%, respectively. Finally, ESBL P. aerug...
Khlood G. Abdelkhalek*, Aly B. A. Badawy, Mohamed Fathi, Elshymaa A. Abdelnaby
...in regulation of spermatogenesis with the attainment of buck sexual developmental capacity.
Keywords | Estradiol, LH, FSH, Resistive index, Testosterone
...
Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2*
...of some common virulence genes and revealed the presence of pfhA, hgbB, tbpA, toxA, sodA, ptfA, sodC, nanB, and oma87 with percentages of 30%, 60%, 10%, 100%, 70%, 90%, 90%, 90%, and 100% respectively. Antibiotic susceptibility tests of the recovered isolates revealed that they were all multidrug-resistant (MDR) with a predominance of resistance to erythromycin and oxytetracycline. In conclusion, these findings evidenced that clinically diseased rabbits have ...
Hailemariam Assefa1*, Tesfahun Dagnaw2
...eus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested fungal species. The oil was obtained by soxhlet extraction method. The maximum oil yield using petr...
Jarosław Pytlewski1, Ireneusz R. Antkowiak1 and Ewa Czerniawska-Piątkowska2*
Weihao Chen1, 2, Zhilong Tian1, Lin Ma1, Shangquan Gan3, Wei Sun2, 4* and Mingxing Chu1*
...ion of a series of clock genes. A number of studies have revealed the role of circadian rhythms in the estrous mode of mammals. In the present study, the expression patterns of six canonical clock genes (Clock, BMAL1, Cry1, Cry2, Per1 and Per2) were analyzed in year-round estrous rams (Small Tail Han sheep, STH) and seasonal estrous rams (Sunite sheep, SNT). The result showed that all six ...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...a through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA (2 mg/L) followed by 65% at 2 mg/L of 2,4-D. Maxim...
Dewi Ratih Ayu Daning1,2, L.M. Yusiati1, C. Hanim1, B.P. Widyobroto1*
...ificant actor for methanogenesis) and Prevotella (a major actor for ammonia production).
Keywords | Galangal essential oil, Cineole, Decreasing methane, Microbial biodiversity, Rumen fermentations, In vitro
...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*
... transcription levels of genes encoding major subunits of pathogenic factors such as fimbriae, flagella, enterotoxin, adhesin and hemolysin under the influence of the AHLs. The results showed that under the influence of quorum sensing AHLs, the biofilm formation ability and adherence ability of ETEC were significantly reduced, and the expression of fliC and other major virulence factors were regulated. AHL signaling molecules can regulate virulence rela...
Altaf Hussain1*, Muhammad Saad Ashraf3, Asim Shamim1, Mohsin Nawaz1, Arslan Mehboob2 and Urooj Fatima2
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
...A, spvC, stn, and qacED1 genes were detected in 75, 16.67, 66.67, and 50% of Salmonella serotypes, respectively. These findings signify the role played by chicken-ready meals, as well as their handling, in the high rate of multidrug-resistant Salmonella isolates and the risks it poses to public health.
Keywords | Chicken meals, Salmonella, Humans, Antibiotics, Virulence, Sequencing
...
Mustafa Ozan Atasoy
...which convey immunogenic genes of interest obtained from vitally important diseases. Herpesvirus of turkeys (HVT) has long been considered a versatile tool owing to its non-pathogenic characteristic, relatively higher transgenic capacity, and enabling the DIVA strategy. Numerous bivalent and multivalent HVT constructs have been generated by various methods and some of these constructs have been made commercially available so far. The efficacy of HVT-vectored v...
Jiao Ma*
... important role in embryogenesis and embryonic development. So far, seven types of cardiac stem cells with different molecular phenotypes and differentiation potentials have been discovered and studied. Increased proliferation and differentiation of these cells in areas of cardiac ischemia is a significant factor in the repair and enhancement of heart injury. This role of endogenic cardiac stem cells can be regulated by factors. Numerous causes, such as paracr...
Zheng Tan*, Li Li, Yuxin Song, Meirong Tian and Fan Jia
...y a key role in the pathogenesis and can be used as indicators for clinical risk stratification of CHD....
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
...ile, Sequencing and phylogenesis of six selected isolates revealed that, all isolates were virulent NDV with amino acid motive 112RRQKRF117 at F protein cleavage site. Furthermore, all isolates were clustered together with NDV genotype VII.1.1 (former GVIId) in a high similarity with previously reported strains from Egypt since 2012 till now, as well as with other neighboring and geographically close countries. In conclusion, the recent NDV outbreaks have been...
Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*
...CEBPA, PRKAG3 and SREBF1 genes and their relationship with fat deposition between fat-tailed (rumped) and thin-tailed breeds were investigated. Two and one SNPs were identified for PRKAG3 and SREBF1 respectively. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin-tailed breed) by Sequenom MassArray. For PRKAG3 gene, a c.1744C>T SNP and a c.1840C>T SNP have been genotyped. For SREBF1 gene, an unkno...
Shaokun Lu1,2, Ronghua Li1,2*, Chunlin Wang1,2, Changkao Mu1,2 and Weiwei Song1
...sequences, some of these genes are associated with important immunological functions. These EST-derived SNP markers will be useful tools for fisheries management and conservation programme of P.trituberculatus.
...
Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3
...nolone resistance (PMQR) genes qnrA, qnrB, qnrS, qepA and aac(6′)-Ib-cr by PCR. The results revealed that 22 isolates (91.7%) harboured at least one PMQR gene, with qnrS being the most frequent (83.3%). The qepA, qnrB and aa (6′)-Ib-cr genes occurrence was 54.2%, 16.7% and 4.2% respectively, while qnrA was not detected in any isolate. The high prevalence of fluoroquinolones resistance, and transferable fluoroquin...
Nour El-Hoda Khayrat Hammad1*, Yousef Y. El-Seady3, Azza E. Hassan1, Sara T. Elazab2, Magdy S. Amer2
...ssion of CYP17A1 and LHR genes were significantly reduced in testicular tissues of FCZ group while aromatase gene was elevated. The administration of LIN oil after FCZ treatment markedly improved the aforementioned alterations caused by FCZ. So linseed oil is capable to ameliorate the fluconazole induced fertility disorders.
Keywords | Male fertility, Antifungal, Medicinal plant, Testosterone hormone, Aromatase
...
Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3
...γ and ifnλ1 genes in WBC; 6) Expression of p53 and gadd45g genes in WBC. The concentration of the viral p27-FeLV antigen in the blood plasma was determined by ELISA. The total DNA and total RNA was extracted from the WBC. The cDNA was obtained using an mRNA matrix. Qualitative and quantitative analyses of FeLV in WBC as well as expression of genes of interest were conducted us...
Ghassan Zahid1, Sania Begum2, Sikandar Almani3, Sahir Hameed Khattak2, Rajesh Kumar Soothar4 and Shakeel Ahmed Soomro4*
...ned all the slow rusting genes complex i.e. Yr18/Lr34, Yr29/Lr46 and Sr2/Lr27. Therefore, detection of slow rusting gene complex in these varieties with the assistance of molecular markers can be utilized for slow-rust resistance. Furthermore, the selected markers can be used as a primary choice for detecting rust resistance genes specifically in the wheat breeding population.
... 
