Identification and Expression Analysis of an Olfactory Receptor Gene Family in the Yemma signatus Hsiao (Hemiptera: Berytidae)
Identification and Expression Analysis of an Olfactory Receptor Gene Family in the Yemma signatus Hsiao (Hemiptera: Berytidae)
Yue Qin Song, Hui Zhong Sun* and Jun Feng Dong
ABSTRACT
Olfactory receptors (ORs) in the dendritic membrane of olfactory cells are the key elements in the molecular recognition and discrimination of odorants. On the basis of female and male antennal transcriptomes of Yemma signatus adults, a total of 66 candidate Y. signatus olfactory receptor genes (YsigORs), including one olfactory co-receptor (Orco), were identified in this study. All the sequences were further validated by cloning and sequencing. Tissue expression profiles of all YsigOR genes in the antennae of females and males were analyzed using real-time quantitative PCR (RT-qPCR). The result showed that some YsigOR genes displayed significant differences in the expression levels between sexes. YsigOrco had the highest expression level in all YsigOR genes; however, the expression level in males was twice as that of females. Our study provides valuable biological information for studying the olfactory communication system of Y. signatus.
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October 2020
Vol. 52, Iss. 5, Pages 1631-2026
