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Identification of LncRNA CASC7/ miR-26/ASPN/TGF-β/Smad Axis in Endometrial Cancer

Identification of LncRNA CASC7/ miR-26/ASPN/TGF-β/Smad Axis in Endometrial Cancer

Xiajun Zhang1, Jie Yang2, Wenjun Zhou1, Zhenshi Chen2, Weidong Wu3, Shaoru Zhang2 and Lihui Wang2*

1Clinical Laboratory, Danyang People’s Hospital of Jiangsu Province, Danyang Hospital Affliated to Nantong University, Danyang, Jiangsu, China
2Central Laboratory, Danyang People’s Hospital of Jiangsu Province, Danyang Hospital Affliated to Nantong University, Danyang, Jiangsu, China
3Anesthesiology Department, Danyang People’s Hospital of Jiangsu Province, Danyang Hospital Affliated to Nantong University, Danyang, Jiangsu, China
 
Xiajun Zhang and Jie Yang are the co-first authors.
 
* Corresponding author: njuwanglihui@163.com

ABSTRACT

Endometrial cancer is the most common gynecological malignant tumor in many countries. TGF-β regulates cell proliferation, angiogenesis and metastasis, and is an inducer of epithelial-mesenchymal transformation. Tumor cells activate the TGF-β/Smad pathway, and inhibiting its signal transduction pathway is an important strategy for tumor therapy. First, we have found lncRNA CASC7 is a poor prognostic marker of endometrial cancer. Then, ASPN was specifically expressed in endometrium and cell specific expression in basophils, among the most similar genes, we found TGF-β3. Moreover, lncRNA CASC7 acts on miR-26, and miR-26 was predicted to regulate ASPN. Next, Except for SMAD2 and XIAP, the expression of ASPN, TGF-β1, TGF-β2, TGF-β3, SMAD3 and SMAD4 genes in normal tissues was higher than that in tumor tissues. ASPN was positively correlated with TGF-β1, TGF-β2, TGF-β3, SMAD3 and SMAD4. Pathway activity shows ASPN and TGF-β1 and TGF-β3 activate EMT. Finally, we speculate that lncRNA CASC7 inhibited miR-26, inhibited ASPN, inhibited TGF-β, and promoted XIAP, to activate SMAD/XIAP pathway, improving the survival rate and invasiveness of endometrial cancer cells. Collectively, this is the first time to describe the mechanism of lncRNA CASC7/miR-26/ASPN/TGF-β/Smad axis in the proliferation and invasion of endometrial cancer cells.

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Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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