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Developmental and Pluripotent Genes in Rat Adult and Neonatal Kidney

Developmental and Pluripotent Genes in Rat Adult and Neonatal Kidney

Sumreen Begum, Atta-ur-Rahman and Asmat Salim*

Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

 
* Corresponding author: asmat.salim@iccs.edu

ABSTRACT

Investigating the role of genes in kidney with respect to their specific mechanism in renal development is imperative to regeneration. The purpose of this study is to analyze systematic identification of genes with respect to nephron lineage and pluripotency during postnatal renal development and at adult stage of rat kidney. In the current study, the mRNA levels of nephron genes were analyzed by reverse transcriptase PCR in neonatal and adult rat kidney tissues. These genes were divided into three categories. Group 1: renal multipotent progenitor genes; Group 2: self-renewal and pluripotency genes; Group 3 pluripotent state regulator genes. In neonates, renal progenitor genes Wt1, Pax2, Cad6, Six2 and HNF1β were significantly expressed with concomitant expression of pluripotency genes. Nanog was highly expressed as compared to Oct4 and Sox2 at neonatal stage. Aicda, Glis1, Tbx3 and Dppa5 revealed higher expression among the regulatory genes in neonates, while Lin28, Klf4 and Stat3 were found with no significant difference as compared to adult stage. The nephron specific and pluripotency genes co-expressed in neonatal kidney and highly influenced by the Aicda, Glis1, Tbx3, and Dppa5. Further, Lin 28, Klf4, and Stat3 are required to maintain their expression states during and after development of rat kidney. Hence, over-expression of these genes may provoke the reprogramming of the adult injured tissue for the mechanism of regeneration.

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Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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