Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
ABSTRACT
Maize is an important cereal crop that is widely consumed all around the world. Until now extensive work has been conducted to determine the efficiency of various explants sources for maize tissue culture, especially the immature embryos. However, the manipulation of immature embryos as explants is hampered due to its unavailability throughout the year and low regeneration response. The present study is aimed to investigate the effect of various explants sources for callogenesis and regeneration in maize (Pioner 3025). The main explants under consideration were: three parts of germinated seedlings, mature embryo and split seeds. Each of these explants was evaluated for its ability to form callus and to regenerate subsequently. For callogenesis from different parts of germinated seedlings, maximum frequency was shown by bulged inter node (45.60 %) along with the regeneration efficiency of 46.23 %. In case of split seed technique, the efficiency for callogenesis was 82% with the regeneration efficiency of 65.46%. For mature embryos the efficiency for callogenesis was 61% while for regeneration it was 78.06%. The results revealed that split seed technique is a preferable choice for explants for callogenesis, while mature embryos exhibit optimum frequency for regeneration in maize (Pioner 3025).
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