Expression Analysis of Oxidative Stress Induced Genes in Liver and Heart Tissues in Response to Doxorubicin
Expression Analysis of Oxidative Stress Induced Genes in Liver and Heart Tissues in Response to Doxorubicin
Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1
ABSTRACT
Drugs used in chemotherapy that target rapidly dividing cells, also exert toxic effects on healthy tissues. Doxorubicin (DOX) is a commonly used chemotherapeutic drug. Oxidative stress plays an important role in DOX-induced toxicity. This study was aimed at analyzing the expression levels of genes that are induced in response to oxidative stress in heart and liver tissues following DOX administration to rats. In this study, DOX was administered to rats intraperitoneally (i. p.) at 3 mg/kg at alternate days for two weeks. Heart and liver tissues were harvested and the mRNA levels of NAD(P)H quinone dehydrogenase 1 (Nqo1), glutathione peroxidase (Gpx1) and isocitrate dehydrogenase-1 (Idh1) were analyzed by RT-PCR. We observedvariable pattern of gene expression was observed. Nqo1 and Idh1 genes were upregulated significantly in heart but not in liver tissue. Gpx1 seems to be unaffected both in heart and liver tissues. It is concluded from this study that toxicity due to doxorubicin is variable in terms of expression of certain oxidative stress induced genes and is tissue dependent. These genes therefore can be a potential target for future treatment of cardiotoxicity induced by doxorubicin.
To share on other social networks, click on any share button. What are these?
October 2019
Vol. 51, Iss. 5, Pages 1599-1997
