Effect of Supplementation of Short-Fatty Acid on PER2 and Genes Related to Volatile Fatty Acid in Isolated Goat Ruminal Epithelial Tissue
Effect of Supplementation of Short-Fatty Acid on PER2 and Genes Related to Volatile Fatty Acid in Isolated Goat Ruminal Epithelial Tissue
Jinlong Cheng1, Jianglan Pei1, Qiaoyun Xu1, Jian Gao1, Zanna Xi1,
Jialiang Ouyang1, Mengzhi Wang1,2*, Junliang Yin2* and Wenju Zhang3*
ABSTRACT
Period circadian regulator 2 (PER2) is a core circadian clock gene associated with metabolism, transport and synthesis of lipids. Here, 3-month-old Boer goats were used to explore the relationship between PER2 and volatile fatty acid-related genes in isolated goat ruminal epithelial tissue explants. Internal and external culture medium was collected at 0, 3, 6, and 24 h to measure pH and VFA concentration to evaluate conditions in vitro, research showed pH at 3 h was significant higher than that at other different time, and pH at 24 h was lower than that in the other three groups; The concentration of acetic acid, propionic acid and butyric acid in culture fluid indicated that the concentrations of acetic acid, propionic acid, butyric acid group and control group were not significantly different (P >0.05) on the time axis. Furthermore, Tissue collected at 0 and 24 h after culture with acetic acid, propionic acid, and butyric acid to measure the expression of biological clock genes such as PER2 and VFA uptake-related genes. Results showed that different VFA treatments inhibited the relative expression of CLOCK and MCT1 genes, a correlation analysis between PER2 and VFA uptake-related genes was also performed and showed that PER2 was positively correlated with AE2, and PER2 was negatively correlated with MCT1 in rumen tissue.
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