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Developmental Changes in Myofibers and Expression Profiles of Potential Regulatory Genes in Slow- and Fast- Growing Chickens

Developmental Changes in Myofibers and Expression Profiles of Potential Regulatory Genes in Slow- and Fast- Growing Chickens

Jia Liu1, Zhen Wang1, Zifan Ning1, Ali Mujtaba Shah2,3,Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Yaofu Tian1, Diyan Li1,Gang Shu2,4, Lin Ye1 and Xiaoling Zhao1,*

1College of Animal Science and Technology, Sichuan Agricultural University, ,611130 Chengdu, China
2Department of Livestock Production, Shaheed Benazir Bhutto University of Veterinary and Animal Science, Sakrand 67210, Sindh, Pakistan
3Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
4Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural
University, ,611130 Chengdu, China
 
Jia Liu and Zhen Wang made equal contributions to this study.

*  Corresponding author: zhaoxiaoling@sicau.edu.cn

ABSTRACT

There are weight and size differences in skeletal muscles of fast- (FG), and slow- growing (SG) chickens, however, the underlying molecular mechanisms responsible for the differences in post-hatch muscle development are unclear. Here, we report on the identification of several candidate genes that may modulate myofiber growth and thus explain some of the differences in the skeletal muscle phenotype of FG and SG chickens. We collected pectoralis major (PM) and gastrocnemius muscles (GM) on d 1, 7, 28, 49, and 70 post-hatch, and measured the weights of the muscles, diameter and, a density of myofibers, and the expression abundances of MyoD, MyoG, IGF-1, and Pax7. The body weight of FG was heavier than SG from d 7 to 70. Their muscle weights and myofiber diameters of FG were also greater than SG (P < 0.05). The expression of MyoG was affected by chicken population, age, and muscle-type. It was the heaviest in PM for FG on d 28, compared with other combinations. The expression of Pax7 mRNA paralleled changed in myofiber density, whereas the expression profiles of MyoG and MyoD were similar to the developmental changes in myofiber diameter. Overall, body weight and muscle expansion of SG chickens were less than FG chickens. MyoG was the possible gene controlling myofiber development as its expression abundances were associated with the muscle development profiles in the SG and FG chickens, respectively.
 

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Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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