Candidate Chemosensory Genes Identified in the Procambarus clarkii (Decapoda: Cambaridae) by Antennules Transcriptome Analysis
Candidate Chemosensory Genes Identified in the Procambarus clarkii (Decapoda: Cambaridae) by Antennules Transcriptome Analysis
Zhengfei Wang1*, Chenchen Shen1,2, Yiping Zhang1, Dan Tang1,3, Yaqi Luo1, Yaotong Zhai1, Yayun Guan1, Yue Wang1 and Xinyu Wang1
ABSTRACT
Olfaction plays a vital role in the survival and reproduction of shrimps. However, very little information is available about Procambarus clarkii, an important aquaculture species. Thus, we conducted the antennules transcriptome of Procambarus clarkii and identified 26 olfactory-related genes, including nine ionotropic receptors, two ionotropic glutamate receptors, ten variant ionotropic glutamate receptors, four cytochrome P450s, and one carboxylesterase. Our study suggested that ionotropic receptors were the main odorant receptors in Procambarus clarkii. Additionally, the key genes that are responsible for olfactory transduction in Procambarus clarkii were identified in the cAMP-mediated olfactory transduction pathway, including Cyclic nucleotide-gated cation channel beta-1, Adenylyl cyclase III, Calcium/calmodulin-dependent protein kinase II and Na+/Ca2+ exchanger. As a whole, our study laid a solid foundation for further functional elucidations of olfactory molecular mechanism in Procambarus clarkii, and provided further insight for a better understanding of olfaction molecular mechanism in crustaceans.
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