BMP4 and Rosiglitazone Improves Adipogenesis of Bovine Fetal Muscle Derived Progenitor Cells
BMP4 and Rosiglitazone Improves Adipogenesis of Bovine Fetal Muscle Derived Progenitor Cells
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
ABSTRACT
Intramuscular fat (IMF) content is one of the most important factors determining beef quality and price. Intramuscular adipocytes develop from mesenchymal stem cells (MSCs) in mesoderm. The mechanisms of preadipocytes differentiate into mature adipocytes to a great extent are clear, but the commitment of MSCs to preadipocytes is largely unknown. In this study, the Platelet-derived growth factor receptor α (PDGFRα) positive progenitor cells were isolated from the longissimus dorsi muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated cells. The result indicated that BMP4 and rosiglitazone could promote adipogenesis and be applied in adipogenic differentiation of fetal bovine derived progenitor cells.
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February 2021
Vol. 53, Iss. 1, Pages 1-400
