miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway
miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway
Sara Sultan Alomran1, Muhammad Nasir Khan Khattak1,2, Amir Ali Khan1,2*, Sallam Hasan Abdallah2, Abeer Maher Fayyad1 and Khalid Bajou1,2
ABSTRACT
Unraveling molecular mechanisms that govern adipocyte formation will lead to a greater understanding of obesity and subsequent treatment. In the current study, we assessed the effects of miR-22-3p, a non-coding RNA, on adipocyte differentiation from telomerase-transformed Mesenchymal Stromal Cells (iMSC3). The transfection of iMSC3 with miR-22-3p suppressed adipocyte differentiation as evident by reduction in lipid content and number of lipid droplets. While AKT3 is reported to be a target of miR-22-3p, our study indicated otherwise. Moreover, while miR-22-3p is known to target more than 500 genes, MAPK has not been identified as one of the more than 500 gene targets of miR-22-3p, though this pathway was upregulated in transfected iMSC3 that differentiated to adipocytes. Finally, miR-22-3p was observed to suppress adipocytes and was involved in adipocyte differentiation through non-targeted MAPK pathway upregulation rather than AKT3-related processes.
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August 2021
Vol. 53, Iss. 4, Pages 1201-1601
