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miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway

miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway

Sara Sultan Alomran1, Muhammad Nasir Khan Khattak1,2, Amir Ali Khan1,2*, Sallam Hasan Abdallah2, Abeer Maher Fayyad1 and Khalid Bajou1,2

1Department of Applied Biology, University of Sharjah, Sharjah, United Arab Emirates
2Human Genetics and Stem Cells Research Group, Research Institute of Sciences and Engineering, University of Sharjah, Sharjah, United Arab Emirates

*      Corresponding author: amkhan@sharjah.ac.ae

ABSTRACT

Unraveling molecular mechanisms that govern adipocyte formation will lead to a greater understanding of obesity and subsequent treatment. In the current study, we assessed the effects of miR-22-3p, a non-coding RNA, on adipocyte differentiation from telomerase-transformed Mesenchymal Stromal Cells (iMSC3). The transfection of iMSC3 with miR-22-3p suppressed adipocyte differentiation as evident by reduction in lipid content and number of lipid droplets. While AKT3 is reported to be a target of miR-22-3p, our study indicated otherwise. Moreover, while miR-22-3p is known to target more than 500 genes, MAPK has not been identified as one of the more than 500 gene targets of miR-22-3p, though this pathway was upregulated in transfected iMSC3 that differentiated to adipocytes. Finally, miR-22-3p was observed to suppress adipocytes and was involved in adipocyte differentiation through non-targeted MAPK pathway upregulation rather than AKT3-related processes.
 

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Pakistan Journal of Zoology

August

Vol. 53, Iss. 4, Pages 1201-1601

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