Aradhana Chopra1, Ravi Shukla2 and Tarun Kumar Sharma2,3*
... single stranded nucleic acid molecules that are considered as chemical rival of antibodies. They are generated through an in vitro evolution process called SELEX. These single stranded nucleic acid aptamers can acquire various secondary and tertiary structures to bind their cognate targets with high affinity and specificity. The ease in synthesis and modification makes them an attractive candidate for a variety of applicati...
Maureen McKeague1, Maria C. DeRosa2*
... single stranded nucleic acid-based molecular recognition elements, are generated through an in vitro evolution process called SELEX. The selection of aptamers with optimal specificity and high affinity remains a challenge, leading to the recent emergence of elegant new approaches and tools for SELEX. In this commentary, we discuss recent advancements in the selection of aptamers....
Muhammad Rizwan Tariq1*, Muhammad Issa Khan1, Aysha Sameen1, Mahr un Nisa2
...3 Poly unsaturated fatty acid (PUFA) concentration that contributes 8.5% of the total fatty acids. Rabbits were reared for the period of eight weeks. Enrichment of 7% flaxseed in the diet of rabbits significantly enhanced growth performance of rabbits and improved poly unsaturated fatty acids in the meat of rabbits as compared to other groups.
...
Hai-Bo Wang, Qiu-Hua Mo, Ze Yang*
..., which combines nucleic acid sequence-based amplification (NASBA) and molecular beacon technique. Recently there have been two major progresses in the field, in which RT-NASBA technique was applied for the detection of diarrhoea related viruses. This mini review overviewed the basic principle of RT-NASBA and discussed the significance of these new findings....
Ali Muhammad*, Muhammad Ayub
...was observed in ascorbic acid (15.18 to 7.21), pH (4.56 to 3.9) and TSS (5 to 2.5). Standard deviation showed the least variation in data. Results show that on nutritional basis RW1 was found best followed by RW2....
Gultaj Hussain1, Abdur Rahman1*, Tariq Hussain2, Siraj Uddin1, Tariq Ali3
E-mail | abdur_rahman@aup.edu.pk
...terial effects of citric acid and lactic acid treated beef were studied previously on Escherichia coli O157: H7 and Salmonella typhymurium. This was aimed to investigate the effects of these acids on the total viable count (TVC) of these bacteria. Three diluted concentra- tions (1.0, 3.0 and 5.0%) of both citric acid and lactic a...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: kohlc@rki.de
...g amount of host nucleic acids, which by far exceeds the number of pathogen nucleic acids in the sample. Subsequently, it is necessary to either decrease the amount of host nucleic acids or increase the amount of pathogen nucleic acids, to allow for detection via metagenomic NGS. This minireview is revising our developed TUViD-VM protocol and selected ot...
Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4
...dex (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role these wild birds can play in the transmissio...
Murtaza Ali*, Yasser Durrani and Muhammad Ayub
...ty, ash content, percent acidity, pH, moisture content, reducing and non-reducing sugars, taste, texture, color and overall acceptability). Statistically minimum decrease was observed in water activity, pH, moisture content and organoleptic characters. While, stable increase was noted in percent acidity and ash content during storage. It was concluded and recommended on the basis of mentioned facts that mulberry fruit dehydr...
Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
...F gene. Two unique amino acid substitutions (K423Q and E426G) were observed in Bangladeshi and Chinese isolates in the partial sequence of N protein.The recent Bangladeshi isolates are somewhat divergent from the earlier Bangladeshi isolate, indicating that the PPRV strains in Bangladesh are continuously changing their genetic character....
Muhammad Siddiq1* and Hamid Ullah Shah2
...e value (PV), free fatty acid (FFA), anisidine value (AV), iodine value (IV), color index (CI) and beta carotene (BC) value of the oils. The results showed considerable variations in all the studied parameters tested in different blends under various stress conditions. Maximum PV (10.76%), FFA (2.44%), AV (2.29), IV (77.09) and CI (0.609) were recorded in sole olive oil, while minimum PV (9.69%), FFA (0.65%), AV (0.88), IV (53.40) and CI (0.146) were noted in ...
Mukhtiar Ahmed, Jana Pickova, Taufiq Ahmad, Muhammad Liaquat, Abid Farid, Muhammad Jahangir
... in the storage of fatty acids in foods. Oxidation occurs by several molecular mechanisms such as generation of reactive oxygen precursors and free radicals. Oxidation affects many interactions among food constituents, leading to both desirable and undesirable products. Food lipids are the foods components that are most susceptible to oxidation, therefore oxidation reactions are one of the major sources of deterioration that occurs during manufacturing, storag...
Neha Singh and Anjana Pandey
... single-stranded nucleic acids known as aptamers. Aptamers are known for their high selectivity and specificity and their ability to form 3D conformations. CELL SELEX is a modification of SELEX where instead of using purified ligand molecules, whole live cells are used as targets, so the aptamers being generated could bind target molecules on live cells in their native conformation and it is possible to know the molecular profile of the cellular surface. Whole...
Rashid Mahmood1*, Saima Asad2, Waqar Ahmad1, Ghulam Sarwar1, Muhamamd Khalid Rafique1, Noor Islam1, Ziyad Abdul Qadir1 and Zain Ul Abiden2
...s were exposed to oxalic acid (3.2%), thymol (0.5 g) and formic acid (65%) on screen bottom boards in winter, spring and summer seasons. The fourth group had screen bottom board trays but without any chemical. There was a trend of fallen mean number of mites 157±6.28, 97±3.81, 132.75±9.76 and 26±1.68 in winter, 160±20, 149±30, 161.75±18.5 and 27.5±1.04 in spring and 188...
Sungil Kim1, Muhammad Riaz2, Amjad Farooq3 and Sungkwon Park4*
..., meat quality and fatty acid composition of pork belly. A total of 50 gilts and 50 barrows (initial BW 26±6.3 kg) were randomly allotted to 2 diets including a corn-based control diet and control plus 9% DDGS. After 105 d administration of experimental diets, growth performance, pork quality traits, and fatty acid composition in loin were analyzed. No significant difference in growth performance and quality traits of...
Aqila Shaheen and Mehwish Matien
...orest soils had slightly acidic pH and lower BD than cultivated land uses. Data combined over locations illustrated that Mollisols contained higher SOC and K, whereas, TN, AP, and BD were statistically similar at both sites. The SOC was positively correlated with TN, AP and K of soil, whereas, SOC was negatively correlated with BD and pH of the soil. There is a large difference in SOC storage and physico-chemical properties mainly in relation to...
Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...zoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motility, plasma membrane integrity, a...
Ambreena Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1*
... contents and free amino acids increased, whereas glycogen and lipid contents were reduced in all deltamethrin-resistant populations as compared to deltamethrin-susceptible population. Soluble Proteins were significantly elevated (79, 100 and 37%) in 4th and 6th instar larvae and adult beetles of Gujranwala, (14, 24 and 14%) in Okara and (14, 13 and 2%) in D.G Khan populations, respectively compared to susceptible population. Free amino <...
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
...ine with any other amino acid. The other mutation determined at nucleotide position 101, was a missense mutation, which substituted serine with phenylalanine at 34th position of extracellular domain of GNRHR. The Ser34Phe mutation was manifested in phenotypic traits such as low concentrations of FSH, LH and T and delayed puberty. In conclusion, mutations in GNRHR may cause delay in male puberty in Pakistani population....
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
...a protein with 114 amino acids that contains the SS14 sequence at its C-terminus. This putative peptide is identical to that generated by the SS1 gene in other vertebrates. Tissue distribution of C. nasus SS1 mRNA was analyzed by real-time polymerase chain reaction (PCR), which demonstrated high expression level in the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages t...
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...ral analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could be obtained. Recombinant Npm or PGA was injected into porcine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those...
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
...onadotropic axis. Citric acid is synthesized and secreted by the prostate gland. Its synthesis is regulated by androgens. As, dose dependent degeneration of prostate gland has been described following intraperitoneal kisspeptin treatment. However, effects of kisspeptin administration on the levels of prostatic citric acid remain elusive till date. The present study, therefore, addresses the effects of 12 day administration o...
Sezgi Arman* and Sema Isisag Ucuncu
...e fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PAS treatment were recorded as negative. Because of numerous supranuclear...
Sezgi Arman* and Sema Isisag Ucuncu
...e fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PAS treatment were recorded as negative. Because of numerous supranuclear...
Ibrar Khan1,2,3,*, Sadia Qayyum2, Shehzad Ahmed2, Kashif Syed Haleem2, Mujaddad-ur-Rehman1, Guang-Lei Liu3 and Zhen-Ming Chi3
... metabolites and organic acids. ...
Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*
...b human glial fibrillary acidic protein promoter (hGFAP-DsRed). This study characterized transgene expression in such transgenic Guangxi Bama mini-pigs and their offspring. Our findings indicate that the hGFAP promoter contains matching regulatory elements for directing specific expression in porcine astrocytes. However, the practical application of hGFAP-DsRed transgenic Guangxi Bama mini-pigs in neuroscience research requires solving the germline transmissio...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...ingosine and other fatty acids. In this study we have evaluated the pro-apoptotic effect of ceranib-2, a human ceramidase inhibitor and of a short-chain C2 ceramide on human lung adenocarcinoma cells, A549 by determining cytotoxicity of these agents by trypan blue assay, apoptosis inducing ability by annexin V and JC-1 staining and ultrastructural alterations by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 c...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
... pathways of arachidonic acid metabolism, changes the survival rate and induces apoptosis in a hepatoma cell line (H4IIE). We determined in vitro survival rate with MTT assay, the morphological changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h an...
Jun Cui, Xiaoxu Zhou, Zhicheng Wang, Derong Kong, Xuemei Qiu, Hongdi Wang and Xiuli Wang*
...polypeptide of 445 amino acids. The calculated molecular weight of crucian carp Wap65 protein (CcWap65) is 50.8kDa, containing a signal peptide cleavage site between amino acids 18 and 19. The CcWap65 is a hydrophilic protein and no trans-membrane topological proteins. The SMART analysis revealed that CcWap65 contains three hemopexin-like repeats (E-value < 0.05). The crucian carp Wap65 was mainly expressed in live...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
...ORF), encoding 164 amino acids with an estimated molecular mass of 17.6 kDa. The LvCypA has four β- strands. Tissue distribution of the LvCypA after real time analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease.
...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
... resistance to Nalidixic acid (100%) and Nitrofurantoin (42.9%). Strict biosecurity measures are required to reduce the incidence of mixed bacterial infection and subsequently reduce the spread of antibiotic resistance genes between bacterial spp....
Abdul Wajid Khalil and Zafar Iqbal
...roximate, mineral, amino acid compositions of the aerial parts and roots of B. procumbens. The result of carbohydrate (75.56±0.046%) of aerial parts was found higher as compared to the roots (31.22±0.05%) while protein (37.46±0.02%) and organic matter (21.25±0.03%) was recorded higher in roots than the aerial parts of B. procumbens. Calcium (309.73±0.06mg/100g), potassium (274.59±0.08mg/100g) and iron (32.58±0.0...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Rassol Bakhsh Tareen2, AsadUllah1 and Zahid Mustafa1
... and enhanced the lactic acid bacteria. In conclusion, supplementation of herbs as feed additive improved growth parameters and present results suggests herbs could be used as alternative to antibiotics growth promoting feed additives.
...
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
...was noted to be slightly acidic with non-significant difference (P>0.05) among seasons. Overall mean viable fungal count was 4.72 × 103±708 with a significant association (P<0.05) among seasons. Out comes from study showed that, 44.8% of the assayed samples were contaminated with A. flavus. From them,48.8% of isolates were capable of producing aflatoxin B1. The study revealed the need of regular mycological monit...
Habib Ullah Khan1*, Saleem Ullah1, Hamid Ullah Shah1, Muhammad Arif2
...rical Conductivity (EC), acidity, total nitrogen and other minerals was studied. pH and EC of the treated wood coal ranged from 1.48 to 11.9 and 3.45 to 28.4µs/dl respectively. Soil EC was lower 96.60 µS/dl in untreated soil while highest 190.10 µS/dl in NSP treated soil. Acidity of the treated soil ranged from 0.97g/L in S to 1.41 g/L in FN treated soils. The highest value (6.58x10-3%) of total nitrogen wa...
John G. Bruno
...pervariable region amino acid sequences of the target antibodies, no docking simulations are possible to test this topological hypothesis, but the present work illustrates feasibility of the approach and paves the way for more sophisticated analysis that could lead to improved rickettsial serodiagnostics and aptamer-based vaccines....
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...agment 1-42 and ibotenic acid into the bilateral hippocampus led to significant learning and memory deficits in the Morris water maze test. Pathology analysis of treated animals showed obvious gliosis and neurofibrillary tangles in the cerebral hippocampal area. These results suggest that the tree shrew can recapitulate the major features of AD pathogenesis. To provide the basis for further studies with this new animal model, we used high-throughput sequencing...
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
... activity of chlorogenic acid isolated from apricot cv. Habi, against different food borne pathogens and quantification of the chlorogenic acid (CA) using High Performance Liquid Chromatography (HPLC). The chlorogenic acid was isolated using resin. The recovered amount of compound was calculated. Aliquots of the extracts at known concentration of chlorogenic acid
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...ctive effects of ellagic acid (EA) on the toxicity of the liver induced by D-Galactosamine (D-GaIN) in rats. With this in mind, the rats were categorized into five groups. The study groups were given saline, 0.2 % dimethyl sulfoxide, D-GaIN, EA plus D-GaIN and D-GaIN plus EA, respectively. In the group given D-GaIN, the following transmission electron microscopic and light microscopic results were found: degenerative changes in the liver tissue, significant de...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...energy metabolism, amino acid metabolism and transport and binding.
... 
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...se of molecular (nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies available to virologists have obviously become more diverse and improved, the challenges have also changed greatly.Detection of plant viruses is becoming more critical as globalization of trade,particularly in horticultural commodities increase. The potential effects of climate change have further aggravated the movement of viruses and the...
Partha Pratim Ghosh and 2N. C. Mandal
Some disease management practices for bacterial wilt of potato
...as conducted in slightly acidic (pH 5.62) loamy sand soil with medium fertility status in a Randomised Block Design with three replicates and seven treatment modules, as described in Materials & Methods. Investigation revealed that T1 (TPS whole tuber planting) and T4 (supervised management - cowdung @ 40 ha-1 at land preparation + seed piece tuber treatment with carbendazim 2.5 gL-1 and Streptocycline @ 1 gL-1 + stable bleaching powder drenching with out ...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...ingly conserved glutamic acid to glutamine at site which was involved in coding of ASF1 like histone chaperone. Further presumption based on structural and functional analysis of mutated protein was anticipated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
...
P.P. Ghosh*, C. Ghosh, B. Mahato, A. Chakraborty, S.K. Bhattacharya and M.K. Bhattacharjya
...L.) production under the acidic soil condition of Purulia district of West Bengal resulted in 20-30% yield loss every year. The present participatory action research was aimed to study the (i) adeptness of integrated module of prophylactic soil disinfection and bio-control, (ii) only prophylactic soil disinfection, and (iii) only curative soil disinfection over uprooting and destruction of diseased plants (farmers’ practice). Results indicated all the ma...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...d other LSDVs. The amino acid sequences of GPCR genes of our isolates contained the unique signature of LSDV (A11, T12, T34, S99 and P199). Phylogenetic analyses showed that the GPCR genes of LSDVs identified from ticks were closest genetically to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission of LSDV. This study showed the role of A. hebraeum ticks for transmission of LSDV. So, tick control ...
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
...lyzed for volatile fatty acids and ammonia nitrogen levels. Acetic acid improved significantly in experimental groups, while propionic acid decreased significantly in T1. Valeric acid and butyric acid decreased significantly in T1 and T2 while isovolaric acid and the ratio of acetic ...
Noor Islam1, Muhammad Amjad2, Ehsan ul Haq3, Elizabeth Stephen1 and Falak Naz4
...us linearus), formic acid at three different concentrations of 25%, 50% and 100% and control were used against T. clareae in naturally infested honey bee, A. mellifera colonies in Honeybee Research Institute, National Agricultural Research Centre, Islamabad, Pakistan. The percentage of infestation by T. clareae on worker brood, number of dead/fallen mites per hive/weekon white formica sheet, percent mite mortality and honey yield per c...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
..., and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 gene without introns was consistent with the structural domains characteristics of this family genes. The translation protein of T2R1 gene displayed with corresponding signal loci and functional domains of this gene family, and the result showed that the taste receptor translated by T2R1 gene belongs to G protein-coupled rec...
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
...cessing techniques using acid, alkali, water treatment and fermentation to prepare processed moiety on pilot scale. All these processed materials were included in experimental ration at a fixed level (20 %). All iso-caloric and iso-nitrogenous rations were offered to experimental animals (n=36, 22 male 14 female Lohi sheep almost evenly distributed in experimental groups) having almost uniform body weight (BW). During 60 days of the trial, no significant diffe...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
...to the G- lineage. Amino acid analysis of NA stalk regions of these isolates illustrated no insertion, deletion and substitutions compared to A/DK/HK/Y280/97 lineage and human isolates (A/HK/1073/99 and A/HK/1074/99). Several amino acid substitutions at various regions of the NA protein were determined with unknown functions and consequences. These findings warrant future studies for analysis of whole genome sequencing of pr...
Ayşe Gül Harlıoğlu*
...ign: justify;">The fatty acid compositions of unfertilized eggs, semen, fertilized eggs, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the unferti...
Fawad Ali1*, Hidayat Ullah2, Ikhtiar Khan1
...ylenetriaminpenta acetic acid (AB-DTPA) showed significant differences between the two studied localities. Linear regression analysis indicates that concentration of the heavy metal (Pb, Cr, Cd, Ni, Zn) in vegetables are significantly correlated at R2 value of 0.956 at P≤0.001 with their extractable concentration in soil. Health risk index (HRI) has been found>1 for Pb in Abelmoschus esculentus while rest of the heavy metals have been noted less than 1 i...
Sadia*, Abdur Rab, Sayyed Hamad Ahmad Shah, Irfan Ullah, Farida Bibi and Islam Zeb
...rop (11.69%), titratable acidity (0.59%), with minimum number of new shoots (16.3), number of new flowers (65.3), fruit weight (53.3g), and fruit volume (48.1cm3) were recorded in plants which were not deblossomed and sprayed with distilled water. It is concluded from the current study that Deblossoming time and GA3 application might be significantly reduced the fruit drop and enhanced the growth of guava tree. ...
Syed Tanveer Shah* and Muhammad Sajid
... 8.26 °Brix) and TSS-acid ratio (12.62 and 11.48) were recorded by the alone application of calcium chloride at 1.0% calcium concentration, respectively. The effect of calcium sources and concentration on the rest of the quality attributes were found non-significant. The means for storage duration showed that freshly harvested fruits resulted in better fruit firmness (5.69 kg cm-2), TSS (8.67 °Brix), ascorbic acid co...
Qudsia Khalid, Safdar Hussain Shah*, Faiza Zaeem and Saad Hussain Shah
...ation of saturated fatty acids (myristic and stearic acids), while unsaturated fatty acids exhibited variable profiles with enhanced oleic acid and significantly reduced linoleic acid. The regeneration capability of adapted line decreased 10 times (20% to 2%), while that of unadapted line kept on increasing (26% to 38%...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...nvestigated based on pH, acidity, ºBrix, viscosity, cloud value, hunter color values, bioactive compounds, microorganisms and enzyme analysis. The grapefruit juice was treated with processing variables of HPP treatment (0, 150, 200 and 250 MPa), temperature (40, 50 and 60 oC), for processing time of 3 minutes and thermal treatment was done in a water bath at 70 oC for 90 seconds. In HPP treatment (250 MPa/60 °C/3 min), significan...
Nargis Bano and Khalid Mahmood Qureshi*
...rry.
...
Zahid Mehmood*, Alam Zeb and Muhammad Ayub
...tes (TSS, pH, titratable acidity, total phenolic compounds, antioxidant activity, ascorbic acid) and organoleptic characteristics (flavor, color, texture and overall acceptability). The results revealed decrease (P<0.05) in pH, ascorbic acid, total phenolic content, antioxidant activity while increase in acidity, total soluble solids during six months...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...ition of alpha linolenic acid (ALA) and docosahexaenoic acid (DHA) on frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5o
Naheed Bano* and Muhammad Afzal
... 324 fish were used. The acidification levels were (0, 1.5 and 3%) whereas the phytase levels were (0, 500 and 1000 FTU). The addition of 3 % citric acid and 1000FTU phytase produced significant interaction effect on calcium and phosphorus digestibility and different body contents of L. rohita. Phytase and citric acid increased the digestibility of calcium and phosphorus with improv...
Mehmet Guvenc1,*, Ayşe Nilay Guvenc3, Ökkeş Yılmaz2 and Mehmet Tuzcu2
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
...ng a predicted 641 amino acid residues with a molecular weight of approximately 71.2 kDa. BmARM-like mRNA showed highest expression in hemolymph, significantly high expression levels in 4th instar, moth, and egg, stable high expression levels in the early embryonic development, relatively high expression levels in the 1st day and 5th day of the pupa stage, and highest expression levels in the molting stage. What’s ...
Ayesha Aihetasham1,*, Muhammad Saeed Akhtar1, Maryam Umer1, Khalid Zamir Rasib2 and Muhammad Imran Din3
...tragole, 11-Octadecenoic acid, methyl ester and 9-Octadecenoic acid ethyl ester. Whereas nine different compounds were identified in extracts of O. basilicum. These were: 1-Isopropyl-2, 2-dimethylpropylideneamine, Camphor, Naphthalene, Thymol, 1,5,5- Trimethyl-6-x methylene-cyclohexene, Hexadecanoic acid, methyl ester, 9,12,15 Octadecatrienoic acid
Ehab Bo Trabi1,2, Xianjun Yuan1, Junfeng Li1, Zhihao Dong1, Assar Ali Shah2 and Tao Shao1,2,*
... with glucose and lactic acid bacteria (LAB) additives. The groups were as follow: silage without additive as a control (c); glucose 1% fresh matter basis (G1); glucose 2% (G2); glucose 3% (G3); Lactobacillus Plantarum (LAB1); Pediococuss acidilactici (LAB2); Lactobacillus rhamnosus (LAB3). All the silos were incubated at 25°C. Then, 90 days later, the fermentation quality of tested silages was analy...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...ifferences between amino acid sequence of C. dromedarius and other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology with the porcine GSTpi enzyme. Gene expression analysis in five camel tissues was examined employing real-time P...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...→arginine (R) amino acid (AA) change was detected at KIT exon 13 in both the white cashmere goat and the black cashmere goat. Moreover, comparison with other species revealed three dramatic amino acid mutation areas. Our results also indicated that c-kit expression was higher in the white cashmere goat than in the black goat, and this significant difference was detected by q-PCR and western blotting. All cashmere...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...ment of intestinal fatty acid binding protein in the pathogenesis of MetS.
...
Serap Gelibolu1,*, Yasemen Yanar2, M. Ayce Genc3 and Ercument Genc4
...le body and fillet fatty acid composition shown no-correlation between treated and control groups (p>0.05). During the course of whole body examination, a positive correlation between MOS-fed and control-fed fish was observed for monounsaturated fatty acids and polyunsaturated fatty acids. However, these observations were not apparent in fillet samples. Profiling of the hepatic fatty
Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
...tion of dilute sulphuric acid pretreatment of peanut shells as substrate for the production of cellulose enzyme using Box-Bhenken design of Response Surface Methodology. The pretreatment of substrate was conducted through Box- Bhenken design of Response Surface Methodology. Three factors with three levels such as H2SO4 conc. (0.6, 0.8, 1%), substrate conc. (5, 10, 15%) and residence time (4, 6, 8h) were employed for pretreatment with and without autoclaving at...
Bushra Mazhar1, Nusrat Jahan1, Nazish Mazhar Ali1, Saiqa andleeb2, Shaukat Ali2*
...media containing ferulic acid as a substrate. Optimum pH for E. hormaechei was 7 and the temperature was 30°C. Using optimum growth conditions and suitable fermentation medium, E. hormaechei can be used at an industrial level as producer of vanillin. Bioconversion of waste residues of rice bran oil to vanillin by bacterial strains is of greater interest nowadays. Bioproduction of vanillin is not only considered natural but also safe for human health....
Hafiz Muhammad Tahir1, Khanum Zahra2, Arooj Zaheer2, Khizar Samiullah3
...nce of constituent amino acid or by inserting silk producing gene in another suitable animal....
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
...opyl-P-benzyl-phosphinic acid), intraperitoneally injected for 12 days, on hematological and serum biochemical profile of 6 week old male albino mice. Blood samples from male albino mice (CGP55845 treated N = 14 and saline treated N = 16) were collected directly by cardiac puncture and used for estimation of hematological and some serum biochemical parameters. The hematological parameters did not show any significant variation between the two groups. From amon...
Soraya Khosravian Dehordi*, Abdolnaser Mohebbi and Kahin Shahanipour
...s (PC) andthiobarbituric acid reactive substances (TBARS),superoxide dismutase (SOD) glutathione peroxidase (GPx) and catalase (CAT) values in spleen were measured. An increase in TBARS and PC levels was obtained in rats' spleen of the Cd-group than controls and nano-Se decreased this effect close to the control. The CAT values of all groups did not differ significantly. The values of SOD and GPx were reduced in splenic tissue of Cd-intoxicated rats, but the n...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
...a 780, 784 and 602 amino acid protein, respectively. Chromosome synteny analyses revealed that CcSox5 and CcSox13 weretightly linked with the etnk gene, which was conserved in all species; however, there were no conserved regions flanking CcSox6. Numerous essential transcription factor binding sites (TFs) were predicted within the 2000 bp upstream of the 5’ end of these genes. These TFs include BSX, BRN4 and NGN–NEUROD, which h...
Ozgur Altan*
... of the study, the fatty acids content in the liquid part, the nutrient content, amino acid and fatty acid profile in the solid part have been analyzed. According to the results obtained, it was observed that the solid part could not be used commercially by substituting the fish meal in a rate of one-to-one, and that it could be included in the formulation as a second class raw material al...
Muhammad Usman Saleem1, Saima Masood1,*, Hafsa Zaneb1, Aneela Zameer Durrani2, Asim Aslam3, Kamran Ashraf4, Habib-ur-Rehman5, Muti-ur-Rehman3 and Muhammad Shabir Shaheen6
...haride (MOS) and organic acid blend (OAB) supplementation individually and in combination on carcass yield, small intestinal microarchitecture and serum biochemistry in Hubbard chicks. A total of 128 one day-old chicks were divided equally in four different groups with each group having 4 replicates. The number of birds per group were 32 whereas the number of birds per replicate were 8.The first group was kept as control (CONT) whereas, the second (MOSG) and t...
Muhammad Ijaz Khan* and Amanullah Jan
... and two levels of humic acid (2 and 4 kg ha-1), alone and in combinations with gypsum (1000 kg ha-1) as inorganic soil conditioner were applied a week before sowing of maize. Design used was randomized complete block with split plot arrangement replicated thrice. Experimental site was Agronomy Research Farm, The University of Agriculture Peshawar. Irrigations regimes were subjected to main plots while soil conditioners to subplots. Results of the two years st...
Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3
...nal function, serum uric acid and blood cell counts of Sprague-Dawley rats. For this purpose, twenty Sprague-Dawley rats were divided in two groups. Group A (n=10) was given distilled water and Group B (n=10) was supplemented berberine hydrochloride with dose of 75 mg/kg body weight/day. 24 weeks post-treatment, blood samples were collected by cardiac puncture from both groups and tested for Hb (%), WBC, RBC and platelet counts, SGPT, alkaline phosphatse, seru...
Fazal Ullah1*, Muhammad Sajid1, Syeda Leeda Gul2, Bibi Zainub1 and Maaz Khan1
...firmness (2.41 Kg.cm-2), acidity (1.50%) and ascorbic acid (34.50%). The storage durations also had significant effects on the quality attributes of sweet orange. However the maximum fruit volume (160.25 ml), juice content (57.00%), firmness (2.89 kg cm-2), ascorbic acid (40.96%) and acidity (1.64%) were noted in fresh fruits. The HWDT of 15 minutes was ...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...uction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots per shoot at an average root length of 3.86 cm. After acclimatization and ...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...antioxidants like citric acid and determining its usefulness by applying on food products. Natural edible color was extracted from T. erecta gave lemon yellow color shade which when applied on candies increased the attractiveness. The spectrophotometric analysis at 474nm of extracted color both in crude form and in candies was found to be stable at 4oC and showed decay in mean lutein concentration at higher temperatures i.e. 25oC a...
Muhammad Asim, Muhammad Asif, Muhammad Munir* and Muhammad Aslam**
...distribution of abscisic acid (ABA) were studied in inoculated and uninoculated plants of soybean [Glycine max (L.) Merr.] from the time of inoculation to root-nodule initiation (192 hours after inoculation). 3H-ABA was applied to the leaves of two soybean cultivars: cv. Williams-82 and its hypernodulating mutant, NODI-3. There was a significant difference in the percent uptake of 3H-ABA between the two varieties both in inoculated and uninoculated plants. A m...
Rahmat Ullah Khan, Abdur Rashid*, Mohammad Sohail Khan** and Erdogan Ozturk***
...y the potential of humic acid (HA) as a low-cost natural fertilizer and to determine its effect on the yield of rainfed wheat crop (Triticum aestivum L. cv. Naseer) at the research farm of Arid Zone Research Institute, Dera Ismail Khan during two successive winter seasons, 2007-08 and 2008-09. The treatments consisted of HA alone (3 kg ha-1 or 1.5 kg ha-1) and in combination with full (60:40 kg ha-1) and half (30:20 kg ha-1) the recommended rates of NP fertili...
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2
...wed that change in amino acid sequence of primary structure of enzyme exists at the saponin binding site, resulting in weak interaction for A. mellifera as compared to T. castaneum enzyme protein. Computational studies inidicate that A. mellifera enzyme had a little binding affinity for saponin as compared to T. castaneum AChE. The amino acid residues of T. castaneum AChE identified at posi...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...ause any change at amino acid level, while the 2nd, 3rd and 4th substitutions at nucleotide level results an amino acid change. The phylogenetic studies established that the Pakistanian HBV Isolates closely resemble the “adrq” strain belonging to “adr” subtype. The study showed that the pre-S2 region is hypervariable and two reported sequences show 100% homology and th...
Faisal Khalil, Khalid Mahmood Qureshi, Ammaz Khan, Fakhar-ul-Hassan and Nabila Bibi*
EFFECT OF GIRDLING AND PLANT GROWTH REGULATORS ON PRODUCTIVITY IN OLIVE (OLEA EUROPAEA)
...ormones like gibberellic acid (GA ), salicylic acid (SA) and naphthalene acetic acid (NAA) alone and/ 3 or coupled with girdling operations may improve cropping potential of existing olive varieties in Pakistan. Therefore, this study was envisaged on olive trees to explore possibilities of inducing fruitfulness through the use of hormones and girdling operations under Islamabad conditions....
Khalid Mahmood Qureshi, Fakhar ul Hassan, Qamar ul Hassan, Usman Shaukat Qureshi, Saman Chughtai and Adnan Saleem*
IMPACT OF CULTIVATION SYSTEMS ON GROWTH AND YIELD OF STRAWBERRY (FRAGARIA ANANASSA) CV. CHANDLER
..., total sugars, ascorbic acid were found significantly higher under tunnel condition as compared to other cultivation systems. The experiment was arranged by employing the Randomized Complete Block Design (RCBD) by using five treatments, each comprising three replications with 20 plants per replicate. ...
Sara Khalid*, Khalid Mahmood Qureshi*, Ishfaq Ahmad Hafiz*, Khalid Saifullah Khan* and Usman Shaukat Qureshi*
EFFECT OF ORGANIC AMENDMENTS ON VEGETATIVE GROWTH, FRUIT AND YIELD QUALITY OF STRAWBERRY
...dia + 400 mgl humic 2 -1 acid; T = planting media + 200 g kg leaf manure; T = planting media + 200 3 4 -1 -1 g kg vermicompost; T = planting media + 200 g kg plant fertilizer and T = 5 6 -1 planting media + 200 g kg bio-compost during 2011-12 at PMAS-Arid Agriculture University, Rawalpindi. Treatment T (soil + silt + FYM) induced 1 positive influence on plant height (15.21 cm), canopy spread (20.37 cm), crown diameter (1.47 cm), fresh weight of plant (10.71 g)...
Rabeea Tariq*, Khalid Mahmood Qureshi*, Imran Hassan*, Muhammad Rasheed* and Usman Shaukat Qureshi*
EFFECT OF PLANTING DENSITY AND GROWING MEDIA ON GROWTH AND YIELD OF STRAWBERRY
...it weight and titratable acidity. A significant increase in fresh and dry weight of leaves, number of leaves, fruit yield in term of fruit number, fruit size and fruit weight, and fruit quality with high ascorbic acid contents were observed. On the other hand, plants grown in silt, sand and FYM (1 : 1 : 1) at both planting densities showed significant increment in vegetative growth resulting in early flowering with more flow...
Adnan Umair*, Safdar Ali**, Muhammad Sarwar***, Kashif Bashir**, Muhammad Javed Tareen**** and Muhammad Asghar Malik*****
ASSESSMENT OF SOME PRIMING TECHNIQUES IN MUNGBEAN (VIGNA RADIATA) : A GREEN HOUSE STUDY
...nal priming by salicylic acid (10 and 20 ppm). Untreated dry seeds were used as control. All the invigoration treatments significantly improved plant vigor in terms of final germination biomass, root, shoot length and nutrient uptake of mungbean seedlings compared to control (non-primed dry seedling). The application of seed priming also improved the protein concentration at the early stage of seedlings. Phosphorous application through priming significantly im...
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...ndent. Naphthaleneacetic acid (NAA) also showed a positive effect on in vitro growth responses (shoot proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the growth in terms of shoot mass in two accessions, 4494(1) Kowar Sufaid and 4472(12) Harker Agoon and again the culture response was genotype dependent. There was a difference in the growth responses of the three accessions studied. The best rootin...
Khawer Jawad Ahmad*, Asif Razzaq*, Khalida Hamid Abbasi*, Muhammad Shafiq*, Muhammad Saleem** and Muhammad Arshadullah***
THYMOL AS CONTROL AGENT OF MITES (VARROA DESTRUCTOR) ON HONEYBEES (APIS MELLIFERA)
...was compared with formic acid 65 % and fluvalinate (strip) as standard control measure against Varroa mite. The mite mortality rate was recorded in honeybee colonies of Apis mellifera during 2011-12 at research farm of Beekeeping and Hill Fruit Pests Research Station, Rawalpindi. Analysis of variance for different treatments indicated (F=3.64, F>P=0.15) significantly different effect among treatments as well as for the control of mite. The average efficacy ...
Nazakat Nawaz*, Malik Shah Nawaz*, Mubashir Ahmad Khan*, Mirza M. Yasin*, Doulat Baig*, Nasir Mehmood Cheema**, Muhammad Amjad** and M. Altaf Sher**
EFFECT OF BORON ON PEANUT GENOTYPES UNDER RAINFED CONDITIONS
...and 4.0 kg B ha as boric acid (H BO ) were applied. Boron fertilizer applications significantly 3 3 increased dry pod yield of all the tested genotypes over control. Dry pod yields of BARD-479, ICG-7326, and ICGV-92023 increased by 14- 20%, 21- 26% and 27- 34%, respectively. Genotype BARD-479 was the most boron efficient user, 83- 88% followed by ICG-7326, 79-83% and ICGV- 92023, 75- 79%. Critical boron concentrations in plant shoot terminals -1 recorded were ...
Syeda Nasreen*, Mehwish Ishaque**, Muhammad Ayub Khan*, Saleem-ud-din* and Syeda Musarrat Gilani*
COMBINING ABILITY ANALYSIS FOR SEED PROTEINS, OIL CONTENT AND FATTY ACIDS COMPOSITION IN SUNFLOWER (HELIANTHUS ANNUUS L.)
...s, oil content and fatty acids composition. Among parental lines, CMS-64, CMS-53, CMS-H55-2-2-1 and CMS-53 were the best general combiners for seed total proteins, oil content, oleic and linoleic acid, respectively. Among males, C-206-R, SF-187R, RHA-295 and RHA-854 were the potential parents exhibiting desirable GCA for seed total proteins, oil content, oleic and linoleic acids, respectiv...
ZafarUllah*, Muhammad Azim Malik*, Muhammad Ansar*, Shahzada Sohail Ijaz* and Muhammad Rasheed*
WINTER FORAGE QUALITY OF OATS (AVENA SATIVA), BARLEY (HORDEUM VULGARE) AND VETCH (VICIA SATIVA) IN PURE STAND AND CEREAL LEGUME MIXTURE
...etergent fiber (NDF) and acid detergent fiber (ADF) reflected quality forage. CP for oats in oats-vetch -1 -1 mixture and barley in barley-vetch mixture was 175 g kg and 170 g kg , -1 respectively. NDF and ADF for oats in oats-vetch mixture were 494 g kg -1 and 341 g kg , respectively; while these values for barley in barley-vetch -1 -1 mixture were 340 g kg and 176 g kg , respectively....
Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***
CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...roviding indole-3-acetic acid (IAA), solubilization of inorganic phosphates, nitrogen fixation and siderophores and other organic acid production, whereas indirectly support plant growth by suppressing plant pathogens. The objective of this study was isolation and characterization of bacterial strains from rhizosphere, endosphere and rhizoplane of sunflower. Thirty six bacterial strains were selected out of 44 from plant roo...
Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**
BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...aline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri dishes. The surviving ones were then homogenized in saline and centrifuged prior to biochemical analyses. Results showed differences in the activities of enzymes and quantities of total protein, sol...
Samina Qamer*, Iram Nasir**, Um-Habiba Zafar**, Salma Sultana** and Tayyaba Sultana**
BIO-CHEMICAL EVALUATION OF HONEY PRODUCED BY APIS MELLIFERA HONEYBEE COLLECTED FROM JHANG, BAHAWALPUR, MULTAN, JHELUM AND KALAR KAHAR AREAS
...1 and 196.96mScm -1;free acidity 25.80 meqkg -1, 26.16 meqkg-1 , 24.97 meqkg -1, 30.25 meqkg , 25.4 meqkg -1; lactone 8.17 meqkg -1, 6.52 meqkg-1 , 6.68 meqkg -1, 5 meqkg -1and 4.08 meqkg-1 ; total acidity, 32.96meqkg-1 ,32.52meqkg-1 ,31.65meqkg -1,35.25meqkg and 28.55meqkg-1 ; proline content,376.64mgkg ,312.8mgkg ,260.17mgkg-1 ,402.57mgkg-1 and286.84-1 gkg . Hydroxymethylfurfural (HMF) content of honey samples were measure...
Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**
ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
...extract (52.72 mg gallic acid eq. / gm of extract). Higher concentration of the extract (25%) significantly decreased seed germination (%), seed germination index, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, seedling relative water content and seed vigor index. It was inferred from findings of the present investigation that aqueous extract of exhibits inhibitory effects on growth and development of and therefore could...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
... were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one isolate was most similar to the newly isolated H3N2 viruses from dogs in China and had the same evolutionary branching. The result provided a foundation for further studies on biological characteristics of CIV.
...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...lypeptide with 327 amino acids. The deduced molecular mass and isoelectric point of Vps26A protein were 38.2 kDa and 6.13, respectively. Glutamic acid had the largest proportion (10.4%) in the primary structure. Homologous sequence alignment and phylogenetic tree analysis indicated that theVps26A protein of sika deer was highly similar to that of Bos taurus. Expression analysis by real-time quantitative RT-PCR reveale...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
...e modified Ziehl Neelsen acid fast staining. Prevalence was calculated along with different risk factors associated with the disease (month, age, sex and season). Results showed highest prevalence (P < 0.05) of the Cryptosporidium in Kohat (33.33%) followed by Lakki Marwat (25%), while the lowest percent prevalence was recorded in Bannu (23.33%). Highest month-wise prevalence was recorded in the month of August (46.6%), followed by July (40%), and th...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
...itamins such as ascorbic acid (vitamin C) to the drinking water or feed of poultry has become a common practice. Broilers are more susceptible to heat stress as compared to other animals. The current experiment was conducted to examine the effect of commercial vitamin C supplement (VC100, 200 mg/kg), on performance of broilers. Two levels of environmental temperatures: normal (22°C) and high (32°C) and two levels of vitamin C in drinking water (with an...
Xiaopeng Tang1,3, Rong Xiang1, Sijia Chen1,3, Shufen Yang1,3, Hu Liu1,3, Rejun Fang1,3,* and Aike Li2,*
...ble protein (WSP), amino acid (AA) and peptide fractions, and the AA digestibility and metabolic energy of fermented cottonseed meal (FCSM) and enzymatic hydrolyzed cotton seed meal (EHCSM) in white leghorn roosters. Firstly, CSM were fermented with Aspergillus niger, or hydrolyzed with alcalase and flavourzyme. Secondly, a total of 32 white leghorn roosters with similar body weight (1.95± 0.14 kg) were randomly assigned into 1 of 4 treatments, 1...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...i> alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dysenteriae IM...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
... and 497–543 amino acid residues of S1 glycoprotein) were amplified by overlap PCR, cloned into prokaryotic expression vector resulting pET-Sf200 and confirmed the construct by sequencing. The recombinant plasmid was identified by restriction enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a molecular weight of 38kDa determined through SDS-PAGE and confirmed by Western...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...ral countries. The amino acid and fatty acid profiles of muscle and calipash of Huaihe River, Yellow River, and Japanese strains of Chinese soft-shelled turtles were analyzed and compared. Calipash had higher concentrations of flavor amino acids than muscle. Muscle tissue of the Huaihe River strain had the highest concentration of pharmacodynamic amino acid<...
Lili Zhang, Yuanxiao Wang, Yili Kong, Hussain Ahmad, Rui Yan, Li Dong, Jingfei Zhang and Tian Wang*
...s at the same age. Fatty acid composition of the longissimus dorsi muscle was not affected by IUGR (P>0.05). Furthermore, b*, L*, and C* values, pressure loss, and the ratio of MyHC IIb to other isoforms of the longissimus dorsi muscle were increased significantly by IUGR (P<0.05). Our results suggested that IUGR resulted in low postnatal growth rates, increased ratio of MyHC IIb to other isoforms, lower water-holding capacity...
Humaira Jabeen and Nazia Jamil*
...terium indicum, Bacillus acidiceler were not able to utilize the coconut oil but produced 5 and 22% bio-plastic when supplemented with 2% glucose after 24 h of incubation, respectively. The percentage of the bio-plastic produced when grown in nutrient broth was very low. The Fourier-transform infrared spectroscopy (FTIR) analysis of the extracted bio-plastic indicated the presence of the C=O functional group specific for the presence of polyhydroxyalkanoat...
Mei Li1, Di Zhang2 and Wan-Long Zhu1,*
... 0, 3.3% and 6.6% tannic acid, respectively. The results showed that E. miletus fed the diets with 6.6% tannic acid increased RMR compared with control on day 14, and reduced the gross energy intake (GEI) and digestible energy intake (DEI). On day 28, E. miletus fed diets containing tannic acid showed no significant changes in RMR, GEI and DEI. The apparent digestibility and ...
Hamza Khan1, Safdar Ali1, Ijaz Ahmad*2, Ihsanullah Khan3, Shujaat Hussain1, Bashir Ahmad Khan4 and Muhammad Suhaib5
...in content (%) and Fatty acid profile. Results showed that SMH-1001 and SMH-1215 hybrids performed best for yield and quality parameters, whereas, SMH-1006 and SMH-0909 were considered best for early maturing traits. ...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
... pressure. Deduced amino acid analysis revealed characteristic mutations at HA antigenic sites besides two other mutations (129∆, I151T) that were found to be stable in recent subclade 2.2.1.1 isolates from humans and chickens. Our results revealed that linking the epidemiological and sequence data is important to understand the prevalence, transmission, persistence and evolution of the virus, and to monitor the circulating AIV strains and emergence of n...
Muhammad Suhaib1, Ijaz Ahmad2*, Masooma Munir3, Muhammad Bilal Iqbal4 , Muhammad Khubaib Abuzar5 and Safdar Ali6
...is crop. While salicylic acid is found to play a helpful role in supporting the plant growth and development under salt stress. Keeping in view these factors a hydroponic experiment was conducted on wheat with two levels of salicylic acid (0.25 mM and 0.50 mM) under two salt levels (75 mM and 150 mM) to assess that whether the salicylic acid is helpful in coping the harmful effects of sali...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...lk with dilute sulphuric acid which was optimized through response surface methodology. Pretreatment conditions were optimized using three variable with three levels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase production was achieved by solid substrate which was conducted in 250mL capacity Erlenmeyer flask ...
Muhammad Suhaib1*, Ijaz Ahmad2, Masooma Munir3, Badar-Uz-Zaman1, Bushra Atta4 and Muhammad Khubaib Abuzar5
...plemented with salicylic acid (0.25 and 0.5 mM) under salt stress (75 and 150 mM). It’s an obvious factor that under salt stress certain reactive oxygen species are produced that damage the vital plant organelles causing disturbances in the normal functioning of the plant and even may lead to death in severe cases. The results of this study conferred that there were genotypic variation found in two genotypes regarding salinity tolerance. SARC-4 was found...
Qasim Iqbal1, Safdar Ali1*, Muhammad Naveed Tahir1, Obaidullah Shafique1, Bashir Ahmed Khan2, Ijaz Ahmad3 and Ihsanullah Khan4
...n content (%), and fatty acid profile. The results showed that significantly highest seed yield (2187.3) kg ha-1 was produced by SF 16003 followed by SF16010 and SF 16002 having (2016.2) kg ha-1 and (1888.2) kg ha-1 seed yield respectively. The Cluster analysis also determined SF 16003 as best hybrid which was at a very close distance from the group of SF16010 and SF 16002. ...
Pasqua Rotondi1, Maria Antonietta Colonna1, Giuseppe Marsico1, Francesco Giannico1, Marco Ragni1 and Anna Maria Facciolongo2,*
...ties, chemical and fatty acid composition of intramuscular lipids. Twenty-one three-week-old kids were divided into three homogeneous groups (n = 7), according to age and body weight, and assigned to one of the following feeding treatments: C) control: commercial pelleted feed; L) pelleted feed containing 3% extruded linseed; LO) pelleted feed containing 3% extruded linseed and 0.6% dried oregano inflorescences. Kids were slaughtered at 60 days of age. Diet di...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...e contents of free amino acids and soluble proteins while contents of total proteins, lipids, glycogen and trehalose were significantly reduced with reference to their control (untreated group). Among carbohydrate metabolizing enzymes, the activities of amylase and invertase were significantly reduced while activity of trehalase were significantly increased after treatment with sub lethal dose of λ-cyhalothrin as compared to control. The metabolic deran...
Asad Sultan1,*, Shahroom Khan1, Sarzamin Khan1, Naila Chand1, Muhammad Shuaib Khan2 and Hamza Maris3
...upplemented with organic acid (OA) at the rate of 0, (OA-0), 0.5 (OA-0.5), 1 (OA-1), 1.5 (OA-1.5) and 2 ml/l (OA-2) in drinking water during the starter period for 21 days. Feed intake was significantly (P<0.05) lower and weight gain and dressing percentage were significantly (P<0.05) higher in the OA-2 group, resultantly, the best feed conversion ratio (FCR) was recorded in the same group. Tibia dry matter, ash, calcium (Ca) and phosphorous (P) were sig...
Muhammad Adnan Khan1*, Majid Suhail Hashmi1, Ali Muhammad1, Muhammad Muneeb1, Haris Bilal1 and Gul Wali2
...ds (TSS), pH, titratable acidity, reducing sugar, non-reducing sugar, ascorbic acid and sensory properties as flavor, color and overall acceptability. Samples were stored for three months and readings were recorded at every 15 days interval. During storage intervals, TSS increased from 48.0 to 51.31°Brix, pH increased 3.82 to 4.25, titratable acidity decreased from 1.02 to 0.86%, reduc...
Yijiang Liu1, Kun Li2, Hui Zhang2, Khalid Mehmood2,3, Muhammad Shahzad3, Houqiang Luo1,*, Muhammad Asif Yaseen4 and Xiong Jiang2,5,*
...ivariable sites in amino acids were ranged from 0 to 5.22%. The current results contributes to the perform prevention of leg problems in the bamboo rat industry.
...
Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair4
... studied consensus amino acid sequences of Meq proteins possess several amino acid mutations associated with the MDV virulence and a unique distortion in the Proline repeats (Proline-to-Alanine) at position 176 in the Egyptian MDV strains. The Phylogenetic analysis grouped the eight analysed sequences with the previously investigated Meq from Egypt (2011-2013) together with the very virulent European and Chinese MDV isolates...
Adel A. Basyouny Shahin,1,* Amira M. Metwally Mohamed2 and Abd El-Reheem A. El Shatter2
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
Tayfun Karataş
... enzymes, thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significant effect on the hepatic ...
Zhuanxia Chen1, Ningping Tao1,*, Xiao Qin1 and Dayong Liu2
...sted, such as free amino acids, nucleotides, trimethylamine oxide and betaine. Results showed that umami was the most prominent flavor. Compared with liver, IMP is the main flavor nucleotide in meat. However, the contents of the umami amino acids and betaine in liver are much higher than that of meat. Additionally, trimethylamine oxide is mainly found in the meat. After steaming, content of IMP in meat increased significantl...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...ed jerky; the free amino acids content of the fermented jerky was higher than the non-fermented jerky; the acid protease activity was slightly higher than the neutral protease activity in the fermented jerky; four and fifteen volatile compounds were detected for the non-fermented and fermented jerky, respectively. The important compounds affecting the flavor of fermented jerky included DL-Glutamic Ac...
Muhammad Nusrullah*1, Muhammad Younus2 and Yasir Nasir3
...ant test for determining acid base balance of the body. Chronic obstructive pulmonary disease is characterized by chronic airflow limitation which is not fully reversible and it can lead to respiratory failure.
Objective: To determine the correlation between arterial and venous blood gases in patients presenting with chronic obstructive pulmonary disease.
Material and Methods: This cross sectional study was conducted at De...
Laiba Shafique1,*, Muhammad Afzal1, Syed Zakir Hussain Shah2, Mehroze Fatima3, Huma Naz4, Qaisra Saddique1
...n fish body with organic acid supplemented diets in Labeo rohita fingerlings. Five experimental diets were prepared containing organic acid (0%), malic acid (2%), citric acid (2%), formic acid (2%), lactic acid (2%) and designed as “OA1, OA2, OA
Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
...nly three produced amino acid changes in the protein sequence. The UPGMA tree showed a clear differentiation between taurine and indicine cattle, except mitochondrial taurine sequences in Lohani and Nari Master breeds. The within-breed estimates of divergence were very low in all breeds except for Nari Master (mixed-bred). The estimates of divergence among breeds were also low for most breed pairs, except for Nari Master and Dhanni. While the overall genetic d...
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...The contents of myristic acid and palmitic acid reduced (P ˂ 0.05), while those of linoleic acid and linolenic acid increased (P ˂ 0.05) in the carcass of all supplemented groups. Moreover, the supplemented groups had lower (P ˂ 0.05) n-6:n-3 ratio than the control group. Among the supplemented groups, HFO supplemented group ...
Rana Zeeshan Habib1, Muhammad Afzal1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Muhammad Bilal4 and Syed Makhdoom Hussain5
...phytase (PHY) and citric acid (CA) supplementation on minerals digestibility of Labeo rohita fingerlings fed on canola meal based diet. Nine experimental diets were prepared by supplementing CA (%) and PHY (FTU/kg) at the level of 0, 0 (control); 0, 1000; 0, 2000; 1.5, 0; 1.5, 1000; 1.5, 2000; 3, 0; 3, 1000; 3, 2000 respectively. Chromic oxide was used as inert marker in diets to estimate mineral digestibility. Fish fed on PHY supplemented diet showed h...
Irfan Ali Sabir1, Saeed Ahmad1*, Muhammad Nafees2, Ahmad Sattar Khan1, Maryam3 and Ishtiaq Ahmad2
...squo;, while, titratable acidity (TA) was lowest in ‘Lambay Alfansu’ and ‘Momi-K’. However, reducing sugars (RS) (4.83%), non-reducing sugars (NRS) (16.38%) and total sugars (TS) (21.21%) were significantly high in ‘Surkha Burma’. Ascorbic acid contents (AA) were high in ‘Momi-K’, whereas, total antioxidants (0.68 mM Trolox g-1), total carotenoids (TC) (52.0 µg 100-1) and...
Muhammad Amir Maqbool1*, Muhammad Aslam1, Waseem Akbar2, Muhammad Waheed Anwar3 and Ehtisham Shakeel Khokhar1
...n current studies. Amino acid sequences of major intrinsic proteins of chickpea, barrel medic and maize were retrieved from NCBI database followed by BLASTP. Pairwise and multiple alignment of sequences was done by using ClustalX software, and phylogenetic trees were constructed by using MEGA5 software. Medicago truncatula found to have total 35 MIPs which further comprised of 10 MtPIPs, 6 MtTIPs, 17 MtNIPs and 2 MtSIPs. Cicer arietinum genome comprised of 41 ...
Amino Acid and Fatty Acid Composition of Freshwater Mussels, Anodonta pseudodopsis and Unio tigridis
Hülya Şereflişan and Beyza Ersoy Altun*
...imate composition, fatty acid and amino acid levels were measured in A. pseudodopsis and U. tigridis from the Lake Gölbaşı. Crude protein and lipid contents were higher in U. tigridis (10.75%, 0.96%) than in A. pseudodopsis (8.63%, 0.77%, respectively), whereas the situation was vise versa for fatty acid compositions. The proportions of Omega-3 (n3)
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...trehalose and free amino acid while total protein and total lipid contents, were significantly increased with reference to their control (untreated group). Among carbohydrate metabolizing enzymes, the activities of trehalase, amylase and invertase were significantly reduced after treatment with sub lethal dose of esfenvalerate as compared to control. The metabolic derangements induced by sub-lethal dose of esfenvalerate suggest that infestation caused by T....
Muhammad Mobashar1, Muhammad Tahir1, Shahbaz Javaid2,*, Muhammad I. Anjum2, Insha Gul3, Nazir Ahmad1 andAbdul Sami1
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...th regulator gibberellic acid (GA3) on the hemolymph contents of Galleria mellonella (Lepidoptera: Pyralidae) was examined. G. mellonella larvae were fed with a synthetic diet. The effects of GA3 on the hemolymph content of these larvae were tested through a diet incorporation assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, car...
Majid Hussain1, Syed Makhdoom Hussain1,*, Razia Iqbal2, Arshad Javid3, Muhammad Mudassar Shahzad1,4 and Muhammad Zubair-ul-Hassan Arsalan1
...ate the effect of citric acid (CA) supplemented Moringa oleifera leaf meal (MOLM) based diets on hematological indices, nutrient digestibility and growth performance of Catla catla fingerlings. MOLM based diet was sub-divided into one control diet (0% CA) and five test diets, each supplemented with 1%, 2%, 3%, 4% and 5% CA, respectively. Chromic oxide as an inert marker was added in the diets to determine nutrient digestibility. The fingerlings w...
Madiha Naz1, Qudrat Ullah Khan1*, M. Jamil Khan1, Obaid Ullah Sayal2, Asim Afridi3 and Aziz Ullah Sayal3
...s and naphthalien acetic acid (NAA) and compared with inorganic soil applied fertilizers. The results of the study showed significantly higher number of branches, chlorophyll content in the treatment recieving inorganic fertilizers, the manures in combination with foliar NPK was comparable. The yield attributes showed that ten fruit weight were greater in the soil applied inorganic fertilizer and it was statistically at par with treatments recieving manure wit...
Safina Naz1, Khushbo Hussain1, Muhammad Asif Zulfiqar2* and Syed Atif Hasan Naqvi3
...id, Salicylic acid (SA), combination of Naphthalene Acetic Acid & salicylic acid and orange oil extract on physical and chemical parameters gave significant response. NAA promotes vine length upto 12.66 feet while chlorophyll content was 72.50 SPAD Value, while fresh weight of leaves is 5.1g, dry weight is 0.41g, while fresh weight of fruit is 121.66 g and fruit length is 14...
Safina Naz1, Muhammad Akbar Anjum1, Syed Atif Hasan Naqvi2*, Bushra Siddique3 and Muhammad Asif Zulfiqar4
... respectively), ascorbic acid in Spinacia oleracea (84.5mg/100g) and carbohydrate and energy values in Daucus carota (11.25g/100g and 55.80 cal, respectively). While, higher mineral contents were recorded for phosphorous (P) in Spinacia oleracea (85.5mg/100g), sodium (Na) and iron (Fe) in Lycopericon esculentum (65.33mg/100g and11.67mg/100g, respectively), and potassium (K) and calcium (Ca) in Abelmoschus esculentus (277.33mg/100g and 81.17mg/100g, respectivel...
Abdur Rehman1* and Shad Khan Khalil2
...uctive stages. Salicylic acid, potassium nitrate and methanol are considered to induce drought tolerance in plants. Field trials were conducted at Agricultural Research Institute Tarnab, Peshawar-Pakistan to study the effects of moisture stress and foliar application of chemicals at different growth stages on canola growth and phenology during 2015-16 and 2016-17. The experiment comprised of four moisture levels (optimum water, 10%, 20% and 30% reduced irrigat...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
...ished by means of acetic acid enema plus restraint stress. The rat fecal moisture content, fecal trait score, and abdominal wall withdrawal reflex (AWR) score were used to determine whether the model was successful. The effects of Wumei Pill on the TLR4/9-NF-kB signaling pathway rats with IBS-D was observed by testing the contents of serum level of tumour necrosis factor-α (TNF-a), interleukin-6 (IL-6), lipopolysaccharide (LPS) as well as colonic mucosal...
Ali Muhammad*, Yasser Durrani, Majid Suhail Hashmi, Ihsan Mabood Qazi, Muhammad Ayub and Saifullah
...physico chemically (pH, %acidity, %moisture, crude protein) and organoleptically (color, taste, texture and overall acceptability) with an interval of one week for storage period of one month. The treatments were T0 (not treated), T1 (neutralized with NaOH) and T2 (neutralized with NaHCO3). Statistical analysis showed that storage intervals and treatments had a significant (p<0.05) effect on physicochemical and sensory quality of neutralized whey. ...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
...nzylidene)amino]hexanoic acidwas synthesized by condensing N,N-dimethyl aminobenzaldehyde and Lysine together using ethanol as a solvent. The Schiff base was isolated and further reacted with Ce(NO3)3.6H2O and La(NO3)3.6H2O, to afford their respective complexes. FTIR, 1H NMR, UV-Vis and ICP techniques were used the characterization and structure elucidation of the prepared Schi...
İnan Kaya1,*, Muhitdin Yılmaz2, Müge Mavioğlu Kaya3,Abdulsamed Kükürt4 and Mahmut Karapehlivan5
...,4-dichlorophenoxyacetic acid (2,4-D). Fifty Capoeta capoeta fish caught in Kars Creek were equally divided into five groups and acclimatized in separate tanks for 10 days. The fish were kept in tanks as follows: Group I (control) was kept in normal water. Group II, III, IV and V were kept in separate tanks containing 0.3 mg/L carbaryl, 0.6 mg/L carbaryl, 10 mg/L 2,4-D and 20 mg/L 2,4-D, respectively. The blood and liver samples were taken from the fish...
Umair Riaz1*, Muhammad Ali Kharal2, Ghulam Murtaza3, Qamar uz Zaman4, Sana Javaid4, Hina Ahmed Malik3, Humera Aziz3 and Zafar Abbas1
...ferent hormones. Caffeic acid is actively involved in plant physiology and mechanisms of stress tolerance primarily utilized by plants for the synthesis of lignin which ultimately thickened cell walls and plant become resistant to ion toxicity sodium and heavy metal stress. It also reconciles the absorption of high energy radiations in mesophyll cells under drought stress, mechanism involves the production of ferulic acid th...
Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1
...roteins, essential amino acids, carotenoids, fatty acids like linoleic acid and linolenic acid, lignans, minerals and various phytochemicals. Due to the presence of two unsaturated fatty acids, these oils can reduce their biological activity on heating, volatilization, and other parameters like oxidation and UV rays. T...
Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3
...ike carbohydrates, amino acids, alkaloids, flavonoids and glycosides. To test the antibacterial activity of the extracted seed oil, Agar well diffusion assay was employed. Effectiveness of the oil against selected bacterial species was measured in terms of zone of inhibition in millimeters. Amoxicillin was used as a positive control. The results showed that Clavibacter sp. and E. coli exhibit resistant to the oil extract while Xanthomonas sp. displayed nil res...
Muhammad Kashif Asghar1, Muhammad Aqeel Sarwar1,2*, Shahid Riaz Malik2, Waqas Ahmad3, Sumaira Zareen4, Safdar Ali5 and Abid Ali6
...d 0.01 mM B) using boric acid as a source of B. The experiment was laid out in randomized complete block design with factorial arrangement in triplicate run. Results from the experiment revealed that all the seed treatments significantly improved achene yield, yield attributes and oil contents of sunflower. However, seed priming with 1 mM B solution was the best treatment in improving achene yield and oil contents of sunflower hybrid. While in case of hybrids ...
*Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1
SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...s, the demand for citric acid production has been increased due to its application in various advanced medical fields, which can only be fulfilled by biotechnological fermentation using agro-industrial wastes. It is a bidirectional beneficial process for cost effective manufacturing of citric acid and management of agro-industrial waste products. This project was carried out in order to evaluate the enhanced production of ci...
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...bulin, creatine and uric acid along with reduction of SGOT/SGPT, Blood Urea Nitrogen (BUN), urea and albumin as compared to control. Treatments with JFE after Cr+6 exposures significantly improved the hepatic and renal functional profiles possibly by partial liver rehabilitation and regeneration. The JFE significantly recovered the histopathological alterations in reno-hepatic tissue by free radicals scavenging and metal chelating abilities due to presence of ...
Dilara Abbas Bukhari1, Abdul Rehman2*
Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...s, 4 alcohols, 3 benzoic acids, ketone, aldehyde, phenyl and amide groups containing compounds. Some of these may be classified into aroma containing compounds....
Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...in along with urea, uric acid and serum transaminases activity after 12 and 24h of TAA administration in albino
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution. Rats of 12h and 24h group were killed after 12 and 24h of TAA administr...
rats. Rats were randomly divided into three groups (n=3) namely control group, 12h group and 24h group. Each
experimental group (12h and 24h) was administered 300 mg/ kg TAA intraperitoneally (i.p) while control received
same volume of normal saline solution. Rats of 12h and 24h group were killed after 12 and 24h of TAA administr...
Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1
Bioaccumulation of manufactured titanium dioxide
... with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid
digested samples were analyzed by Inductively Coupled Plasma Mass
Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in
various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was
recorded to be 5.7033 and 3.7133 μg/Kg respectively,whereas, maximu...
digested samples were analyzed by Inductively Coupled Plasma Mass
Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in
various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was
recorded to be 5.7033 and 3.7133 μg/Kg respectively,whereas, maximu...
Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2
An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...s i.e.,
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for sacch...
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combinations thereof to
breakdown the lignocellulose into its components. Pre-treatment process changes
cellulose morphological, chemical and physical features, making it prone to
enzymatic attack for sacch...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
...mining, automobile, lead-acid batteries, and building material.Toxic metals are bio-accumulated by insectivorous bats due to their foraging habits and high trophic level. There is a growing interest on the conservation of bats throughout the world as they play an important role in controlling insect population. We measured cadmium (Cd), copper (Cu) lead (Pb) and zinc (Zn) concentrations in bat tissues through atomic absorption spectrophotometer. Our findings s...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
... psychotropic and lactic acid bacteria, Pseudomonas and Enterobacteriaceae), lipid peroxidation (TBARS), total volatile basic nitrogen (TVB-N) and pH values on quality during 12 days. The decrease in bacterial growth activity, physicochemical values (TVB-N, TBARS and pH) and prolonged shelf life in a natural preservative dependence were observed in the fillets. Generally all the test parameters, such as microbiological, physicochemical as well as the lo...
Gangchun Xu1,2, Fukuan Du2, Yuyu Wang2, Yan Li2, Zhijuan Nie2 and Pao Xu1,2,*
...d a protein of 388 amino acids. The 5′ and 3′ untranslated regions were 269 bp and 139 bp, respectively. The full length ortholog PTGES2b was 1,457 bp and contained a 729-bp ORF that encoded a protein of 242 amino acids. The 5′ and 3′ untranslated regions were 402 bp and 326 bp, respectively. One polyadenylation signal (AATAAA) was present 14 nucleotides upstream of the poly(A) tail in both the...
Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4
...n;36.10 mg/100g K. Fatty acid characterization of groundnut cultivars through gas liquid chromatography revealed six fatty acids namely palmitic acid, oleic acid, linoleic acid, arachidic acid, eicosenoic acid and behenic a...
Sabi-ur-rehman1, Farooq Azam1, Shaheed Ur Rehman2, Tasbeeh Ur Rahman3, Ayeza Mehmood4, Afshan Gohar5 and Abdul Samad5*
...rpan, conocarpol, gallic acid, ellagic acid, ellagitannin, castalagin, quercetin, myricetin and syringetin etc. The review expresses the ethanomedicinal potential of this plant specie as well as its importance in modern medicines. ...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
...he blood lipid and fatty acid profiles and reduced the percentage of fat of rabbit meat. Rabbits were reared for the period of two month in three groups. Slaughtering of rabbits was done after 8 weeks. Followings the method illustrated in previous studies fatty acid and physico-chemical analysis were performed. The pH texture, fat, protein, blood lipid profile and PUFA concentration was significantly affected by the inc...
Pingping Cang1, Mingming Zhang1, Guo Qiao1,Qirui Sun1, Dehai Xu3, Qiang Li1, Xinghua Yuan4 and Wenbin Liu2,*
...05). The essential amino acids, non-essential amino acids and total amino acids contents of gibel carp muscle in BFT group were higher than those in control group. Bioflocs were composed with 29.8% CP, 3.2% CL and 19.1% ash at day 60. CP content was appropriate, and LP was lower for gibel carp. Economic profitability analysis included analysis on gross revenue, break-even production, net p...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
...nic bacteria in terms of acid producing and biofilm. The antibacterial activity of capsaicin was evaluated by the minimal bactericidal concentration, change of pH in the first 12 h and OD values of biofilms. Besides, fluorescent images of exopolysaccharides and living bacteria of Streptococcus mutans were measured with confocal laser scanning microscopy (CLSM). In our study, capsaicin showed an outstanding performance in the inhibition of
Rizwan Ullah Shah* and Iqbal Munir
...l of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four levels of indole butyric acid IBA (0.05, 0.1, 0.2 and 0.5 mg/l). Results showed that at NAA 0.05 mg/l + BAP 0.07 mg/l supplements in MS medium significantly effective in callus induction and days to callus in cotyledon and for both cotyledon and hypocotyl were obs...
Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1
...ogenous use of Salicylic acid (SA) and Benzothiadiazole (BTH) as elicitors enhanced the resistance in mungbean plants by triggering the SA pathway. Induced resistance was assessed by evaluating the appearance of symptoms and detection of virus titter through ELISA. Different concentrations of SA and BTH were exogenously applied to activate the inherent resistance of mungbean by the production of defense associated compounds. All treatments were supportive in s...
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...e with multi basic amino acid 112KRQKR/F117 characteristic of velogenic strains. However, phylogenetic analysis revealed clustering of all tested strains within sub-genotype VIg. Three isolates were assessed for pathogenicity based upon the mean death time (MDT), the intracerebral pathogenicity index (ICPI) and the intravenous pathogenicity index (IVPI) in chicken. The ICPI and MDT revealed that all tested isolates were of moderate virulence (Mesogenic) in chi...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
...ilicum) and ascorbic acid in heat stressed broiler chicken. A total of 360 (Hubbard) one-day old broilers were reared under heat stress (38 ± 1°C) and randomly divided into three groups i.e. A, B and C as control, basil seed at 5g/kg and ascorbic acid at 200 mg/kg supplementation, respectively. Improvement of intestinal villus seen in basil supplementary group (P>0.05), and significant increase in th...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ins, tazocin and fusidic acid. Proper screening of MBLs producing GNRs should be done from clinical specimens along with antimicrobial susceptibility testing for developing an effective treatment strategy and thus better control of infections caused by these bugs in future.
...
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...ochemicals and salicylic acid on the growth of Penicillium digitatum causes citrus green mould by using 7 plant extracts alone and in combination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by Chabir (33.60%) and Garlic (29.16%), while minimum growth inhibition was rec...
Doğukan Ölmez1 and Gonca Alak2,*
...ycerol (TAG), free fatty acids (FFA), cholesterol (CHOL), monoacylglycerol (MAG), diacylglycerol (DAG) and phospholipid (PL) values were determined (p <0.05). After 90 min of hydrolysis in muscle, both FFA and MAG-DAG started to collapse, the TAG ratio in total fat content increased relatively.
...
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
...chnology produced fulvic acid (BFA) as a functional feed additive. Four BFA products produced by lactobacillus, bacillus, yeast and fermented BFA from above 3 mixed bacteria were added by a proportion of 1.5% to the conventional feed. Then the Sprague-Dawley rats were fed by normal feed and 4 different BFA feed for 4 weeks. It was observed that the counts of neutrophils, white blood cells, and monocytes was significantly decreased in the lactobacillus BFA grou...
Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1
...) wild strains of lactic acid producing bacteria (LAB) were screened, out of one hundred and forty-four (144) bacterial strains isolated from different local food groups, on the basis of stress tolerance assays. Identification of the screened strains was further confirmed by 16S ribosomal DNA sequences and strain level differentiation was carried out by sugar fermentation tests. These strains were further analyzed for their ability to produce lactic
Mahroze Fatima1*, Muhammad Afzal2 and Syed Zakir Hussain Shah3
... terms of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activities. The minimum value of TBARS was recorded in VE150 group, which was increased again with supplementation of high vitamin E levels. Similarly, adequate supplementation levels (VE1000, VE1500) reduced the superoxide dismutase (SOD), catalase and glutathione peroxidase activities, which were increased by feeding high vitamin dosages. Supplem...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
...2bp coding for 323 amino acids was PCR amplified from Geobacillus thermodenitrificans DSM-465 was PCR amplified and recombinant plasmid pET22b-Asn was prepared. L-asparaginase was produced in BL21 (DE3) RIL codon plus strain of E. coli as heterologous protein under 0.3mM IPTG. The recombinant enzyme was purified by DEAE-Sephadex colum based anion-exchange chromatography. On SDS-PAGE, the enzyme exhibited a molecular weight of about 36 KDa. Its sp...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
...31 bp, encoding 77 amino acids. The third locus in all tested amino acid sequences contains a Ser residue that corresponds to amino acid position 631, the virus resistance site, in the chicken Mx gene. The third locus in all tested amino acid sequences are Ser and corresponds to the chicken 631 amino acid positi...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...omer like D-galacturonic acids. In this study, polygalacturonase from grape skin was purified by salting out with ammonium sulfate, gel filtration on Sephadex G-75 column and ion exchange on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalacturonase activity was found to be 4.5 and stable within pH range 3.5-5.5. Temperature dependent studies revealed te...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...be developed. Tolfenamic acid (TA) is a nonsteroidal anti-inflammatory medicine stimulating apoptosis in cancer cells like pancreas, oesophagus and lungs. This study aims to determine the role of TA in cell viability and apoptosis in glioma cells (T-98G) and androgen-sensitive human prostate cancer cell line (LNCaP). In our study, 1, 5, 10, 25, 50, and 100 μM doses of TA were applied to T-98G and LNCaP cell lines for periods of 24 and 48 h. Subsequent cell ...
Hamid Ali, Muhammad Arshad, Ibad Ullah Jan, Muhammad Zamin*, Junaid Khan, Ikram Ullah and Mushtaq Ali
...ntrations of gibberellic acid and micronutrients. Experiment was laid in Randomized Complete Block Design (RCBD) using two factors i.e. gibberellic acid and micronutrients with three replications. Both the factors gibberellic acid (GA3) and micro nutrients had their significant effects. The best performance was found in the interaction of GA3 and micronutrients for plant height (53,21cm), ...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by disk diffusion method. Double-disk synergy test (DDST) was also performed for detection of Extended Spectrum β-Lactamase (ESBL)-producing organisms. As a result, majority of poultry isolates was susceptible to most of the tested antibiotics and highes...
Fraza Ijaz1*, Umair Riaz2, Shazia Iqbal3, Qamar uz Zaman4, Muhammad Furqan Ijaz5, Hina Javed1, Muhammad Amjad Qureshi1, Zuhra Mazhar3, Ahmad Hassan Khan6, Hassan Mehmood7 and Ijaz Ahmad8
...rgent fiber (33.45%) and acid detergent fiber (26.56%) gave significant results as compared to control (T1). Results indicated that the combined application of Rhizobium species and Tryptamine performed better by improving growth and yield and quality parameters. It is concluded that precursor-inoculum combination is an effective approach and should be tested in different ecologies. ...
Muhammad Sohail1*, Asad Sultan2, Said Sajjad Ali Shah3, Muhammad Sajid1 and Adnan Khan4
... and sorghum. The phytic acid contents were found highest in both varieties of sorghum (0.85% and 0.87% respectively) as compared to the wheat varieties (0.78 % and 0.80%) and corn varieties (0.72 % and 0.71 respectively). Retention and digestibility of OM from maize cultivars was higher (0.841 and 0.836 respectively) followed by wheat varieties (0.811 and 0.806 respectively) while the sorghum varieties (0.782 and 0.779 respectively). The digestibility coeffic...
Shengjie Zhou1,2, Pengfei Wang1,2, Chao Zhao1,2, Mingjun Fu3, Jian G. Qin4, Lihua Qiu1, Zhenhua Ma1, 4,* and Maoshang Lin1
...div>The liver-type fatty acid binding proteins (L-FABP) gene in golden pompano Trachinotus ovatus larvae was cloned in the present study. The full length of L-FABP cDNA from golden pompano was 604 bp, including a 5’-untranslated region (UTR) of 154 bp, a 3’-UTR of 69 bp and an open reading frame (ORF) of 281 bp. L-FABP encoding a polypeptide of 126 amino acids with a predicted molecular weight of 14...
Aliya Ayaz, Gohar Ayub, Maqsood Khan* and Syed Aizaz Ali Shah
...Factor A was gibberellic acid concentrations i.e. 0, 20, 40 and 60 mgL-1, while factor B was comprised of dipping durations i.e. 0, 6, 12, and 18 hours respectively. Gibberellic acid concentrations and dipping durations had significant effect on the growth and yield of bitter gourd. Gibberellic acid concentration at @ 60 mgL-1 significantly reduced days to emergence and days to first flowe...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
...ition score and ascorbic acid supplementation on the semen quality of breeding bulls. A total of 8 adult (≥2 year) bulls (2 each from Jersey, Friesian, Sahiwal and cross bred) were selected with body condition score (BSC) at a scale of (0-5). The results showed that mean semen volume was significantly (P<0.05) high in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was signifi...
Muhammad Afzal1, Nighat Sultana1, Ali Hassan1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Syed Makhdoom Hussain4, Muhammad Bilal5 and Majid Hussain2
...i>) juveniles fed citric acid (CA) acidified phytase (PHY) pretreated soybean meal (SBM) based diet.Basal SBM based diet was supplemented with two levels of CA (0 and 2%) and two levels of microbial PHY (0 and1000 FTUkg-1) in factorial arrangement resulting in the formulation of four experimental diets. Chromic oxide (1%) was added as an inert marker in the diets to evaluate the nutrient digestibility. Important w...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...ane proteins and nucleic acids. The PCA analysis also exhibited that these three groups were well distributed in different areas. In conclusion, the microoperation of ICSI caused some changes in the metabolism of embryos. Raman spectroscopy is a valuable technology for assessing embryonic metabolism.
...
Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3*
...o be 95 and 96% at amino acid level for BnSHP1-like and BnSHP2-like genes, respectively. The phylogenetic reconstruction of SHP1 and SHP2 homologs from other species conglomerated BnSHP1-like and BnSHP2-like into their respective clades. Semi-quantitative RT-PCR revealed overlapping expression of both the BnSHP1-like and BnSHP2-like transcripts in flower and siliques while no expression in the leaf tissues was observed. A strong expression for FUL gene was det...
Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3
...eotide and deduced amino acid sequences for VP2 hypervariable region of selected five IBDV field isolates were determined and compared to well characterized reference and vaccine strains worldwide. The IBD virus was detected in 9 out of 16 (56.25 %) investigated chicken flocks. Sequence analysis revealed that the analyzed Egyptian isolates identified as very virulent Infectious bursal disease virus (vvIBDV). The identity between these Egyptian isolates and the...
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
...ransition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K rarely found mutations in PIK3CA gene in NSCLC population. Present study can be proved as road map in setting PIK3CA mutations as potential therapeutics target for NSCLC in non-smokers.
...
Amjad Ali1*, Wiqar Ahmad1, Muhammad Zeeshan1, Farmanullah Khan2 and Muhammad Motasim Billah1
...ne triamine penta acetic acid (AB-DTPA) extractable soil Fe after < 2, 2-5, 5-10 mm particles size treatments and the control was 2.48, 2.06, 1.99 and 1.81 mg kg-1, Zn was 0.41, 0.36, 0.33 and 0.31 mg kg-1, Mn was 1.23, 1.05, 1.02 and 0.89 mg kg-1, and Cu was 0.51, 0.38, 0.37 and 0.26 mg kg-1, respectively. Bizote-N and Rhizogold treatments OM (1.5 and 1.48%) showed 3 and 0.7% increase over the control (1.47%), respectively,. Bizote-N induced increase in...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
...us (CDV) without nuclear acid extraction, we established a direct amplification TaqMan real-time (RT)-PCR method (DARPM) to detect CPV and CDV in clinical samples. We compared this new method against real-time PCR/(RT)-PCR and it showed no cross-reactivity with other pathogens. Sensitivity testing showed the minimum detection limits of real-time (RT)-PCR were 7.44×101 copies·μL-1 (CPV) and 4.20×101 copies&...
Comparison of Yolk Fatty Acids in Market Available Black-bone Chicken Eggs and Ordinary Chicken Eggs
Suhu Duan, Liuan Li*, Zaiqiang Li, Mengran Qin, Zhenzhen Fan, Keyan Zhang, Qian Wang, Zhongmou Zhang, Bailiang Yang
...ses the content of fatty acids in yolk of the commercially available ordinary chicken eggs and Brand A and Brand B black-bone chicken eggs, thus providing theoretical and practical references for selective consumption of chicken eggs. The results have shown that a total of 18 types of fatty acids are detected in 3 types of chicken egg yolk, including 6 types of saturated fatty acids (SFA),...
Rashid Iqbal1,2, Mathias Neumann Andersen1*, Muhammad Aown Sammar Raza2, Muhammad Adil Rashid1 and Salman Ahmad2
... and DI. Higher Abscisic acid (ABA) production under PRD reduced stomatal conductance, net photosynthesis and transpiration rate. Magnitude of osmotic adjustment (i.e. leaf water, osmotic and tugor potential) was lowest and highest under FI, DI and PRD, respectively. However, PRD had lower shoot biomass and grain yield per plant as compared to FI but higher than the DI. Despite improving the WUE, PRD tended to compromise the yield of wheat crop but PRD is reco...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...which tris-citric acid extender (TCA) was supplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 ...
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...s had increased sugar to acidity ratio. The correlation coefficient (r) has also confirmed the influence of fungal disease on postharvest physiological disorders and organoleptic properties. The overall acceptability of citrus had strong relationships with pH (r = -0.95**), total soluble solids (r = 0.96**), Acidity (r = 0.93**) and sugar/acid ratio (r = ...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...se, glycogen, free amino acid and trehalose contents was also recorded. The results indicated that contents of glycogen, trehalose, total lipids, free amino acids, soluble proteins and total proteins were significantly deceased in both larval instars except the free amino acids that increased in 4th larval instar of both populations. The glucose contents increased in both larval...
Matiullah Khan*, Shameemul Sibtain Shah, Atiqullah Khan, Muhammad Sarwar and Shoaib Ahmed
...28% total P2O5. The free acid contents were equal and lower (5%) in the prepared SSP for mesh size 160 and 200, against 6.3% when mesh size was 120. The SSP application as P source increased grain yield and total dry matter yield 2.5% and 4.7% over DAP, respectively. Similarly, SSP application enhanced P uptake of crop by 5.6% over DAP, however, no increase in postharvest soil P concentration was recorded. Seed inoculation with PSB significantly increased grai...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...these changes, the amino acid is changed that leads to significant change in the folding of 3D protein structure of clinical sample, while in subclinical samples, showing the same variation in overall 3D protein structure analysis of TNF-α gene. The association between polymorphism identified within the TNF-α gene with mastitis reported in this study revealed that SNPs has potential to serve as a molecular marker for screening of mast...
Laiba Shafique1,*, Muhammad Afzal2, Syed Zakir Hussain Shah3, Mahroze Fatima4, Huma Naz5, Saif ur Rehman1, Youchuan Wei1 and Qingyou Liu1,*
...veness of dietary formic acid and vitamin D3 on growth performance and muscle proximate analyses of grass carp (Ctenopharyngodon idella). Four experimental diets viz. FD1, FD2, FD3 and FD4 having formic acid (%) and vitamin D3 (IU/Kg) 0, 0; 2, 0; 0, 5000 and 2, 5000, respectively were prepared. Fish were fed with experimental diets for 90 days. Growth performance of fish was obser...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...n poultry based on amino acid sequence at the proteolytic cleavage-site of the HA gene with PAKSSR/G. Our findings highlight the potential risk of ND and AI in poultry and continued active surveillance is needed to monitor the transmission of these viruses.
...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...ectively. The full amino-acid sequence of the monomericpeptide was analyzed by sequential Edman degradation using a protein/peptide sequencer. The antibacterial activity of the protein monomer in antlerplate was studied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial ability. The molecular mass and purity of this polypeptide was 18.970kDa and 90%. Amino ...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
... genes changed the amino acid from leucine to histidine and methionine to valine, respectively. These polymorphic results suggest that Marecha camel, a major breed in Pakistan, has genetic variability in the GH and GHR genes. These results suggest that Marecha camel has genetic variability in growth-related genes and identified polymorphisms may be helpful for association studies and future selection programs.
...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
Abdulkareem Mohamed Matar1, Moez Ayadi1,2, Hassen Mohamed Sbihi3*, Imeddine Arbi Nehdi3, Mutassim Mohammad Abdelrahman1 and Riyadh Saleh Aljumaah1
... milk composition, fatty acid profile, physical properties, and tocopherol contents of milk fat from dairy ewes. Sixty-four multiparous Najdi ewes were selected, randomly distributed into four groups (n = 16), and fed four different diets. The diets were composed of traditional feed (TF, barley and alfalfa hay) and three complete feeds with different compositions (CF1, CF2, and CF3). Twenty-four Najdi ewes were randomly divided...
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
...n the extract were fatty acid esters and aliphatic hydrocarbons. Methyl-(7Z)-7-hexadecenoate, methyl-(9E)-octadecenoate and methyl tetracosanoate were found to be the principal fatty acid esters, while E-pentatriacont-17-ene and 2,4-dimethylpentane were identified as predominant aliphatic hydrocarbon compounds. The propolis extract has exhibited remarkable antimicrobial activity against certain strains of bacteria and yeasts...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain2, Afia Muhammad Akram1, Arshad Javid3, Majid Hussain5, Syed Zakir Hussain Shah4 and Asma Chaudhary1
...) based diet. The phytic acid’s presence in plant by-products decreases the bioavailability of nutrients to fish, resulting in poor fish growth and low nutrient digestibility in the body. Experimental diet was divided into six groups and were supplemented with graded levels (0, 300, 600, 900, 1200 and 1500 FTU kg-1) of phytase. Cr2O3 was incorporated in all diets at the rate of 1% as a non-digestible marker. The fingerlin...
Naveed Ahmed1*, Abdul Waheed1, Farrukh Siyar Hamid1, Imtiaz Ahmed1, Muhammad Abbas Khan1, Sohail Aslam1, Muhammad Adil Younis2, Seemab Ali1, Nadia Khan1 and Madiha Bashir1
...ntrations of Gibberellic acid (GA3) (0, 20, 40 and 60 ppm). Experiment was laid out on Randomized Complete Block Design (RCBD) with three replications. Data showed a significant difference among most of the growth parameters. Maximum head weight (2306.7 g), stem length (42.66 cm), number of leaves plant-1(15.73cm), root length (27.53 cm), yield (55.63 t/ha) was recorded for cultivar cabbage red with the application of 60ppm of GA3
Muhammad Usman Akhtar1,2, Abdul Qayum3, Anshan Shan1,*, Shuli Chou1, Hyeonsoo Jo4, Syed Waqas Ali Shah4 and Ishfaq Muhammad5
...etergent fiber (NDF) and acid detergent fiber (ADF) intake were higher (P < 0.05) in goats fed 70RDP diet than those fed 30RDP diet. Nutrients digestibilities (DM, CP, NDF and ADF) were significantly higher (P < 0.05) in goats fed 60RDP diet than those fed 30RDP. Goats fed 70RDP diet produced higher milk yield/d than those fed 30RDP diet.These results revealed that Beetal goats fed 70RDP showed increased nutrient intake and digestibility and proved to be...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...2 genes, including fatty acid binding protein 4 (FABP4), acyl-CoA dehydrogenase short/branched chain (ACADSB), ELOVL fatty acid elongase 3 (ELOVL3) and solute carrier family 27 member 1 (SLC27A1), were altered upon ABCA1 down-regulation using RNA interference (RNAi). Overall, ABCA1 expression influenced triglyceride metabolism in BMECs by regulating the expression of related genes in the lipid metabolism pathway.
...
Muhammad Izhar Shafi* and Muhammad Sharif
...d with and without humic acid on P availability. Three different rates of humic acid as 0, 5 and 10 kg ha-1 with three different P sources as nitrophosphate (NP), single superphosphate (SSP), and di-ammonium phosphate (DAP) at a recommended rate of 90 kg P2O5 ha-1 were applied. Soils amended with different P fertilizers with and without HA showed mix trend in AB-DTPA extractable P ...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...p>, α-ketoglutaric acid and ascorbic acid. Liquid chromatography coupled with tandem mass spectrometry was used to monitor the hydroxylation of proline. The results showed that rhP4H specifically hydroxylated proline residues only in the Gly-X-Y structure but did not react with free proline. We hypothesize that rhP4H can be engineered to control structural properties of recombinant collagen by selecting a certain degre...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
...substitution of an amino acid from Cysteine to Arginine.That Cys/Arg polymorphism may serve as authoritative genetic marker for selective improvement of growth traits in camel. These results suggest that Marecha camel has genetic variability in the IGF-1 and may be helpful for preservation strategies and future selection programs.
...
increasing in growth parameters).
