Genetic Diversity Analysis of Major Intrinsic Proteins (MIPs) among Maize (Zea mays L.), Chickpea (Cicer arietinum L.) and Barrel Medic (Medicago truncatula L.) by using Phylogenetic Inferences
Genetic Diversity Analysis of Major Intrinsic Proteins (MIPs) among Maize (Zea mays L.), Chickpea (Cicer arietinum L.) and Barrel Medic (Medicago truncatula L.) by using Phylogenetic Inferences
Muhammad Amir Maqbool1*, Muhammad Aslam1, Waseem Akbar2, Muhammad Waheed Anwar3 and Ehtisham Shakeel Khokhar1
ABSTRACT
Major intrinsic proteins (MIPs) of chickpea (Cicer arietinum L.), barrel medic (Medicago truncatula L.) and maize (Zea mays L.) were targeted in current studies. Amino acid sequences of major intrinsic proteins of chickpea, barrel medic and maize were retrieved from NCBI database followed by BLASTP. Pairwise and multiple alignment of sequences was done by using ClustalX software, and phylogenetic trees were constructed by using MEGA5 software. Medicago truncatula found to have total 35 MIPs which further comprised of 10 MtPIPs, 6 MtTIPs, 17 MtNIPs and 2 MtSIPs. Cicer arietinum genome comprised of 41 MIPs which were consisted of 10 CaPIPs, 12 CaTIPs, 15 CaNIPs and 4 CaSIPs protein sequences. Genome of Zea mays was carrying 36 ZmMIPs which were categorized into 12 ZmPIPs, 13 ZmTIPs, 8 ZmNIPs and 3 ZmSIPs. Phylogenetic trees were constructed separately for every sub-families of barrel medic, chickpea and maize. PIPs were consisted of two main groups for all of three crops which were named as PIP-A and PIP-B. NIPs clustered into six subgroups which were named as NIP-1, NIP-2, NIP-3, NIP-4, NIP-5, and NIP6. TIPs and SIPs were scattered in four groups and entitled as TIP-1, SIP-1, TIP-2, SIP-2, TIP-3, SIP-3 and TIP-4 respectively. Collective phylogenetic tree was constructed for all of MIPs which showed that PIPs, TIPs, NIPs and SIPs of all of three crops clustered in respective groups and no outliers were present.
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