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Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2

1Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, Indonesia 16915; 2Department of Animal Science, Faculty of Agriculture, Lambung Mangkurat University, Jl. Jenderal Ahmad Yani Km. 36, Banjarbaru Kalimantan Selatan 70714.

 
*Correspondence | A Nurlatifah, Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Jalan Raya Jakarta-Bogor, Bogor, Indonesia 16915; Email: aeni001@brin.go.id

ABSTRACT

The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) antioxidants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in maintaining motility, viability, and membrane integrity. The research concluded that the administration of NEZ at a dose of 20 mg/100 ml extender and EZ treatment at 50 mg/100 ml extender represents the optimal dose to preserve sperm quality until day four of dilution. In addition, the NEZ antioxidant showed better-improved quality of spermatozoa in the liquid semen of Garut rams compared to the EZ antioxidant.
 
Keywords | Antioxidant, Ascorbic acid, Catalase, Çhilled semen, Ram, Spermatozoa

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Advances in Animal and Veterinary Sciences

April

Vol. 12, Iss. 4, pp. 586-801

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