Isolation and Characterization of a Pseudomonas Putida Bacteriophages
Isolation and Characterization of a Pseudomonas Putida Bacteriophages
Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3
ABSTRACT
Two phage isolates for Pseudomonas putida were isolated from soil cultivated with potato by the enrichment culture technique and signed as PPI and PP2. The phages were propagated and purified by polyethylene glycol, 6000-sodium dextran sulfate 500 two phase system. The two phages had different ultraviolet absorption spectra. Results of the lytic pattern of both phages showed that, they had a restricted host range. Particle morphology of the isolated phages was determined and it was found that the two viruses have tadpole shape, which phage PPI has an isometric head (135 nm) and long non-contractile tail (225 nm) whereas phage PP2 has an isometric head (121 nm) and long contractile tail (202 nm), the tail possing an outer sheath (135 nm) and neck (27 nm). Protein patterns of the isolated Ps. putida phages were analysed by SDS-PAGE and the data revealed that phage PPI had 16 structural proteins, 6 are known and the other are extrapolated, but phage PP2 had 13 structural proteins 5 are known and the others are extrapolated. Both viruses have one molecule of the nucleic acid DNA with molecular weight of 2223 bp and 2559 bp for PPI and PP2 respectively.
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