Semen Quality of Ongole Crossbred Bull with Gonadotropin Realizing Hormone (GnRH) Treatment
Semen Quality of Ongole Crossbred Bull with Gonadotropin Realizing Hormone (GnRH) Treatment
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
ABSTRACT
Administration GnRH to bulls is expected to improve semen quality. This study aims to determine the effect of giving the hormone GnRH in various doses on the quality of semen in Ongole Crossbreed (OC) bulls. The study used 18 OC bulls with three GnRH treatments, A (one dose/5ml/animal), B (half-dose/2.5ml/animal), and C without GnRH. The GnRH was administered once by intramuscular (IM) injection. Semen collection was carried out for 16 weeks; divided into two periods, namely 8 and 16 weeks after GnRH administration. Parameters that were measured include volume, acidity, color, consistency, mass motility, individual motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A showed cream color and thick consistency over treatments B and C. The mass motility and sperm concentration of treatment C with the dose and period of GnRH administration resulted in the lowest values significantly different (P<0.05) than treatments A and B, so it affects the total spermatozoa and the total motile spermatozoa. Sperm viability in one dose of treatment A resulted in the highest value significantly different (P<0.05) than treatment B and C with spermatozoa viability of 78.42±2.46% (A), 76.14±1.52% (B), and 65.57±8.26% (C) in the 16 weeks. Nevertheless, the abnormalities and libido between treatments and different periods did not show any difference. It was concluded that the administration of GnRH at a dose of 5 ml/IM can increase the mass motility, individual motility, sperm concentration, total spermatozoa, total motility of spermatozoa, and viability in Ongole Crossbred bulls.
Keywords | The Ongole Crossbred bulls, GnRH, Semen quality
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