Reviewed by David Svolba, Fitchburg State University, USA email: dsvolba@fitchburgstate.edu

Science, Religion and Culture
...e should not be taken as expression of skepticism on Leiter’s part concerning the case for religious tolerance. Leiter affirms the strength of that case in the book’s opening chapter.

...

Sandeep Satapathy and Rupam Ghosh

...h IL-6 signalling is the expression acceleration of sequestrome 1(SQSTM1) / aggrosome and colocalization of HDAC6 to the cytoplasm. The key player HDAC6 is investigated for modelling several intracellular processes that come into action to bring out the viral clearance. The shuttling of HDAC6 takes place from nucleus to cytoplasm and vice versa controlling the overexpression of IFN-beta and ISGs which subsequently triggers ...

Qingzhong Yu

 

...sertion site for optimal expression of a foreign gene without compromising the replication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression...

Rabbi Paul Shrell-Fox

...e. Some have found other expressions of their talents, while others anxiously await the opportunity to find alternate means of financial support. Specific issues of Jewish dietary practice, Sabbath observance and daily prayer are addressed, as well as an exploration of the rabbis’ connection to the Jewish people, despite waning practice.

...

Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2

 

... has its most signi cant expression in maize than other cereal crops. Exploitation of this phenom- enon of superior performance of F1 progeny over their parents not only trans gured maize breeding schemes but also constituted the foundation of maize seed industry. Evaluation of per se performance of parental lines and estimation of heterosis and inbreeding depression are prerequisites to start hybrid development program. In this context, we explored heterosis ...

Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*

...tandard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several possible improvements in the selection of reference genes are discussed.

...

Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2

...tive gene actions in the expression of traits. Performance of hybrids was environment-specific. However, heterobeltiosis estimates ranged between -7.9 and 25.90 for leaf area, -6.76 and 130.8 for number of cured leaves kg-1, -10.8 and 17.1 for yield, -16.0 and 9.94 for grade index, -10.4 and 68.66 for nicotine and -26.89 and 31.25 for reducing sugar, respectively. The current study identified KHG24/Spt G 28; KHG21/NC606 and Spt G 126/KHG24 as best c...

Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1

...cted mice. Moreover, the expression of the goblet cell mucin gene, muc2 was upregulated after treatment with the root extract. Based on our results, it could be concluded that, S. persica root extracts acts as anticoccidial agent.

...

Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1

...l investigations for the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E demonstrated liver injury characterized by centrilobular hepatocellular necrosis, CD68, and Bax expressions were also...

Siyu Yang1, Fukuan Du2 and Pao Xu1,2*

... which demonstrated high expression level in the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward. This study provides some basic evidence that SS1 may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus.

...

Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2

...) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could be obtained. Rec...

Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan

...time PCR, cytokines gene expression were examined in response to infection with two strains of Egyptian H9N2 (namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently comparing to RSF/1 a...

Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*

...ocyte-specific transgene expression of transgene have made significant contributions to understanding astrocytic structure and function in vitro and in vivo, evidence suggests that mouse models are limited and sometimes do not fully replicate the complete spectrum of neurological phenotypes seen in human diseases. On the contrary, pigs, especially mini-pigs, show exciting potential for modeling human CNS diseases due to similarities in body size, anatomy, life...

Muhammad Saeed* and Iftikhar Hussain Khalil

...hat non-additive genetic expression were pre-dominant for most of traits studied. Line BRF3 and testers ZM04 were good general combiners for most of traits under consideration, while F1 hybrids B4N11 × PS08, B4N11 × JZ, BRF1 × ZM04, BRF1 × KT10 were best specific combiners observed for several important traits including grain yield plant-1 which can be subsequently utilized in future wheat breeding to develop high yielding new wheat cul...

Zamarud Shah, Safdar Hussain Shah, Asad Jan and Gul Shad Ali

...confirmation, HsfA1d overexpression lines (OX1, OX2 and OX3) were evaluated for their response to heat stress. Overexpression lines on average showed 33.26% less electrolyte leakage after induction of heat stress at 42°C compared to wild type plants (WT). No significant decrease was recorded in the chlorophyll content of overexpression lines whereas significant decrease was recorded in...
Wan-Long Zhu1 and Guang Yang2,*
...amic neuropeptides genes expressions on body mass regulation under different photoperiods in Eothenomys miletus, body mass, food intake, serum leptin levels and hypothalamic neuropeptide neuropeptide Y (NPY), Agouti related peptide (AgRP), pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript (CART) expressions were measured. The results showed that short photoperiod reduced body mass and body fat ...

Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns

 

...already reported the overexpression of these genes against IBDV infection in susceptible cells. The protection ability of ISGs to safeguard chicken cells against the virus could be potentially applied for vaccine production, better vaccination protocols and infection control in the future.

...
Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*
... Ci2) presented a higher expression in antler mesenchyme of female reindeer, but the other two (Ae1, Af3) were the opposite. The results of Blast in GenBank showed that they shared high homologies with SMAD5 (95%), COL6A3 (95%), SLC17A5 (93%), TPM4 (99%) and HMGB1 (98%), respectively. Quantitative RT-PCR validation of gene COL6A3 showed that the expression in both sexes was consistent with the results of mRNA differential di...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...ne. The mRNA and protein expression levels of Ldh-c in plateau pika skeletal muscles were determined by real-time PCR and Western blot. The LDH activities, lactate contents and ATP levels in skeletal muscle were compared between the experimental group and the control group. The results showed that 1) the expression levels of Ldh-c mRNA and protein were 0.804±0.059 and 0.979±0.176, respectively; 2)...
Jun Cui, Xiaoxu Zhou, Zhicheng Wang, Derong Kong, Xuemei Qiu, Hongdi Wang and Xiuli Wang*
...d in liver, with limited expression observed in intestine, skeletal muscle and kidney. CcWap65 was significantly up-regulated in the liver of crucian carp after heat stress, suggesting that increase in Wap65 gene may be related to high water temperature stress and play important roles in high water temperature environment of crucian carp.
...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
...e analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease. 
...
Andrea Rezic1, Ivica Boskovic2, Piera Lubinu3, Marina Piria1, Tihomir Florijancic2, Massimo Scandura3 and Nikica Sprem1*
...o obtain a more accurate expression of form and better differentiation between the sexes.
...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...n promoter. The in vitro expression of recombinant plasmid DNA was performed by transfection of 293T (HEK) cell line. The HA protein was analyzed using SDS-PAGE followed by Western blot and immunofluorescence assays. The pCAGG-optiH9 vaccine efficacy was evaluated by intramuscular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chicken...

Shams Ur Rehman, Muhammad Arif and Abdul Mateen 

 

Soil-borne viruses in major potato growing areas of Pakistan
... characteristic symptoms expression, bait test, infectivity assays and double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Both the viruses were prevalent in Hazara and Malakand divisions. The average symptoms based incidence in Hazara divi-sion for PMTV was 8.83% and TRV was 6.80% while in Malakand division the average symptoms based inci-dence for PMTV was 8.46% and TRV was 9.95%. DAS-ELISA based average incidence in Hazara of PMTV and TR...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1 
...d regulating the hormone expression in animals. In current study, Pit1 gene was screened for polymorphic sites in Sahiwal cattle. Samples from Sahiwal cattle breeds were sequenced using six set of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fragments were aligned with the help of BLAST for SNPs identification. This is a first report toward genetic screening of...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...WGD) of common carp. The expression patterns of all GST genes were established in various tissues, including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution post duplication. Overall, the whole set of GST genes provide essential genomic resources for future biochem...
Wenqiao Hui1, Jishun Tang1, Dejian Zhu1, Qian Ban2* and Sheng Chen1*
...hways which modulate the expression of hypothalamic genes involved in reproduction and metabolism. We found that leptin significantly upregulated the LEPR in the adipose tissue of goat at the puberty onset stage. Moreover, we observed that LEPR, JAK2, STAT3 (P<0.05), KISS1 (P<0.05) were also upregulated in the hypothalamic tissue, except NPY, which was downregulated. Taken together, it is, therefore, inferred that probably leptin secreted in the adipose ...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...ed into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HEK (Human Embryonic Kidney) cell line for in vitro expression of the recombinant plasmid DNA. The confirmation of H5 protein expression was performed using SDS-PAGE followed by Western blotting and immunofluorescence assays. The humoral immune response was...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and  Kui Chen2,*
...studied the differential expression spectrum of tissues in combination with bioinformatics analysis so as to understand the possible functions in response to chemical sensation behavior of taste receptors. The receptors when compared with T. rubripes, there was 99.2% identity in two kinds ORF sequences, with only 7 bases different, and there were 5 amino acids different between speculated fusion proteins. Under bioinformatics analysis, the T2R1 g...

Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan 

...nism was involved in the expression for maturity trait, plant stature, spike length, spikelet spike-1 and grains spike-1. Negative intercept of regression line indicated the major role of over-dominance in the inheritance of plant height, spikelets spike-1while for grains spike-1 the positive intercept of regression line indicated predominance of partial dominance in inheritance.F1 cross combination FS × JNB were observed as good specific combiners for s...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...LET for 15 days, and the expression profiles of seven gonad differentiation related genes (Cyp19A, Cyp19B, Arα, ERα, Dax, Foxl2, Dmrt1) and gonad histological changes were examined. The results showed that LET could inhibit oocyte growth in female and promote the development of testes in males. For the gonad differentiation related genes, the expression profiles of Cyp19A and Cyp19B show a pattern of increase in ...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...beta glucuronidase (GUS) expression with average frequency of 40.56% was noticed with three days of co-cultivation period while two days of co-cultivation period resulted 24.07% transient GUS expression. Addition of 100 mgL-1 L-cysteine in co-cultivation medium increased the transient transformation by 33.9% compared to control treatment. It has also been observed that cysteine enhanced the expressio...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
... ailment.
...
Rehana Shahida1, Tasnim Farasat1, Shagufta Naz1,* and Shahjahan2
... study aims to study the expression of TNF-α among impaired glucose tolerant (n=35), diabetic subjects (n-35) and age matched healthy control (n=35), and to identify the relationship of inflammatory cytokines with insulin resistance and the molecular mechanisms involved in the interaction of atherosclerosis and hyperglycemia within the context of chosen pro atherogenic parameters including plasma glucose level, serum levels of insulin, IL-6, and TNF-&alp...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
... mRNA showed highest expression in hemolymph, significantly high expression levels in 4th instar, moth, and egg, stable high expression levels in the early embryonic development, relatively high expression levels in the 1st day and 5th day of the pupa stage, and highest expression l...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...liferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regu...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
..., we report the cloning, expression and characterization of the glutathione transferase isoenzyme P1-1 gene from Camelus dromedarius (CdGSTP1-1). The coding sequence was cloned using RT-PCR. Sequence analysis demonstrated significant differences between amino acid sequence of C. dromedarius and other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that Cd...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...ve addressed the diverse expression of the KIT gene and its protein ingoats of different fur colors. The effect of KIT mutations on KIT protein expression was examined in white cashmere and black cashmere goats. A single A→G missense mutation in exon 13 differentiated cashmere goats with different colors. Only a histidine (H)→arginine (R) amino acid (AA) change was detected at KIT exon 1...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...rative study on BPI gene expression in various tissues between Rongchang pig and Landrace, we confirmed the expression of pig BPI gene in various tissues to determine the difference expression of antibacterial related gene among indigenous breed (Rongchang pig) and artificially cultivated breed (Landrace), The Rongchang pig (0 day old, 28 day old, 120 day old) and Landrace BPI (0 day old, ...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...esponsive proteins which expression level more than 1.5-fold were identified, where 50 proteins were up-regulated and 28 proteins were down-regulated; The identified proteins were categorized into 16 classes, which are mainly including energy metabolism, gene expression regulation, oxidation-reduction reaction, cellular component and stress responses, and the stress proteins including Mn-superoxide dismutase and thaumatin-li...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...> and FKBP1B mRNA expression levels in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition, genes mRNA and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay was performed to observe the cells proliferation. The...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...ieve. In this paper, the expression of AR mRNA in various parts of the oviduct was analyzed by qPCR. Oviductal structure was observed by H&E staining. The distribution of AR in the cells was detected by immunohistochemistry. The results showed that the expression of AR mRNA was found in the infundibulum, magnum, isthmus, uterus and vagina of the oviduct. The expression of the AR mRNA i...

Malak Atiq Ullah Khan* and Fida Mohammad 

...fect than G and E on the expression of phenotype for all traits. Average over nine environments, genotype G-79 exhibited maximum values for tillers m-2 (181 tillers); grains spike-1 (71 grains); grain yield (4862 kg ha-1) and thus appeared as leading genotype for yield and production traits. Similarly, G-79 had higher grain yield in all test environments except E-03 i.e. E-01 (4537 kg ha-1); E-02 (4840 kg ha-1); E-04 (5035 kg ha-1); E-05 (4976 kg ha-1); E-06 (...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
...ng nervous system. Their expression has been seen in neural stem cells as well as differentiating neurons. However, despite their importance in development, a relatively low number have been characterized for freshwater fish. In this study, we were able to gain three full-length SoxD sequences from transcriptome sequencing data of carp: CcSox5, CcSox6 and CcSox13 (Cyprinus carpio SoxD).These genes were verified by sequ...
Tania A. Shakoori1,*, Soumble Zulfiqar2, Khadija Irfan Khawaja3 and Faisal Masud4
...ted. We compared genetic expression of Sirt1, AMPK, Akt, and mTOR in adipose tissue from mice treated with NaB versus placebo. Four groups of mice (n=27) mice were administered oral NaB, intraperitoneal (i.p) NaB, oral placebo or i.p placebo for 16 days. RNA was isolated from the epididymal fat pads and relative genetic expression of AMPK, Sirt1, mTOR and Akt was measured using real time PCR. Oral NaB group showed decreased ...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...ridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immune and neuro-modulation in the female reproductive system.
...

Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar

...ntial for aromatase gene expression that leads to aromatase and estrogen production. Danazol is a synthetic androgen whereas Letrozole is aromatase inhibitor. This study was conducted to compare the mean decrease in visual analogue scale (VAS) pain score with Danazol versus Letrozole for the pain management of endometriosis in females. A total of 140 females fulfilling inclusion criteria were registered and an non-probability purposive technique of sampling wa...

 Hidayatullah, T. Mahmood, M. Farooq, M. A. Khokhar and S.I. Hussain*

* Corresponding author: hidayatu2003@yahoo.uk.co.uk

PLANT GROWTH REGULATORS AFFECTING SEX EXPRESSION OF BOTTLE GOURD (LAGENARIA SICERARIA MOLINA) PLANTS
...00 µmol l ) on sex expression of bottle gourd (Lagenaria siceraria Molina) plants cv. Faisalabad Round was investigated under field conditions in National Agricultural Research Centre, Islamabad. Plants sprayed with distilled water were considered as control. Among all foliar agents, the response of -l GA3 3 and MH was found better. Exogenous application with 30 µmol l GA maximally increased the pistillate flower production as compared to control. ...

 Tasawar Sultana*, Farah Deeba* and S.M. Saqlan Naqvi*

HIGH-THROUGHPUT AGROBACTERIUM-MEDIATED TRANSFORMATION OF MEDICAGO TRUNCATULA IN COMPARISON TO TWO EXPRESSION VECTORS
...vector was compared with expression vector from pCAMBIA series over-expressing same gene (pCOsRGLP1). A lower number of explants generated hygromycin resistant plantlet for instance, 18.3 with pGOsRGLP1 vector as compared to 35.5% with pCOsRGLP1 vector. Transformation efficiency of PCR positive plants generated was 9.4% for pGOsRGLP1 while 21.6% for pCOsRGLP1. Furthermore 24.4% of explants -1 generated antibiotic resistant plantlet on 20 mgl of hygromycin whic...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...6A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
... cloned into prokaryotic expression vector resulting pET-Sf200 and confirmed the construct by sequencing. The recombinant plasmid was identified by restriction enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a molecular weight of 38kDa determined through SDS-PAGE and confirmed by Western blotting. The recombinant truncated protein was then...
Wei Dang1, Ning Xu1, Wen Zhang1, Jing Gao2, Handong Fan1 and Hongliang Lu 1,*
...shock protein 70 (Hsp70) expression in six lizard species to investigate the variation in Hsp70 response contributing to thermal adaptation. At first, we collected three lizard species of the genus Takydromus from different geographical locations. We found that either the constitutive expression pattern in different organs or the inducible expression pattern under higher ambient tem...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
...ls were used as host for expression studies of AMYSBS. Recombinant AMYSBS produced as inclusion bodies was transmitted to soluble active form by denaturing the insoluble protein using 8M urea followed by refolding through gradual dialysis. The refolded enzyme exhibited optimum activity at 55 °C between pH 8-9. The effect of metal ions on the activity of AMYSBS showed that Co2+ remarkably enhanced the enzyme activ...
Muhammad Tariq Zahid1, Farah Rauf Shakoori2, Soumble Zulfiqar3, Khalid A. Al-Ghanim4 and Abdul Rauf Shakoori3,4*

Nadia Mubarik1, Aqib Iqbal1*, Iqbal Munir1 and Muhammad Arif2 

...ent as well as the Lhcb2 expression in the leaves under both irrigation conditions; however, the effect was more prominent under water stress. In the control plants, water stress decreased RWC (-57%) and Lhcb2 transcript abundance (-44.37%) but increased proline (9.02 fold) and sugar content (3.42 fold) after 20 days. Significantly higher RWC (82.00 and 55.67%), proline (4.13 and 13.29 nmol·g-1 FW) and sugar content (51.19 and 102.00 mg·g-1 FW) u...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...an index or the relative expression of TLR4 and IgM (P>0.05). Furthermore, correlation analysis showed a positive relationship between the mRNA expression of TLR4 and concentrations of TNF-α and IL-12. These results revealed that although there were no significant effects of LPS injection on TLR4 mRNA expression in lymphoid organs, the response to LPS may not ...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...n. Relative quantitative expression of mRNA transcripts related to apoptosis (Bax and Bcl-2), embryo development (LAMA1, IL-6 and FGF4) and stress (HSPB1) were examined. The results showed that 10% and 20% SFF supplementation exerted no effect on maturation and cleavage percentage whereas 40% SFF supplementation significantly decreased maturation and cleavage. SFF supplemented at 10% concentration showed a significant increase in blastocyst development when co...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
...tudy reports on the gene expression and molecular characteristics of three members of the CC family of chemokines: 1) dual chemokine ligand CCL17; 2) homeostatic chemokines ligand CCL21; and 3) CCL24 in response to bacterial challenge in large yellow croaker (Larimichthys crocea). The results revealed that the immunological effect of CCLs in the croaker is weaker than that of other fish, and revealed the infectivity of the large yellow croaker. Thus, th...
Habib-ur-Rehman1, Masood Ahmed Siddiqui1,*, Abdul Qayyum1, Arifa Bano1 and Naeem Rashid2
...ity, due to low level of expression or the proteolytic machinery of the host. Herein we report expression in Escherichia coli and purification of metal ion independent type II pullulanase from Pyrobaculum calidifontis. Thermophilic origin and metal ion independency of the enzyme reported in this study make it a potential candidate for starch hydrolyzing industry.
...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...t cells. The recombinant expression of some proteins in E. coli produces inclusion bodies which are misfolded proteins. The objective of this study was soluble expression of IFNα2-Tα1fusion protein in E. coli using pET-SUMO vector and determination of its biological activity. SUMO-IFNα2-Tα1 was successfully expressed in soluble form with IPTG induction to final concentration 0.5 mM at 37...
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
... performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed that neurexophilin 1 was expressed in cytoplasm as well as cell nucleus of chicken fibroblasts and Hela cells. Western-blot an...
Yang Yang1,2, Yun Liu1, Mingxu Zhou1 and Guoqiang Zhu1,*
...ichia coli flagellin expression, E. coli F18ab strain 107/86 was modified to either express acyl-homoserine lactone (AHL) synthase (QS-I) or deleted for autoinducer 2 (AI-2) expression (QS-II). AHL expression and deletion of luxS (AI-2) both inhibited flagellin expression, as measured by motility assays, bacterial gene
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
...in our local population, expression of HMG-COAR is not investigated. The objective of our study was to examine the expression of HMG-CoAR in primary breast carcinomas and determine its association with ER/PR expression.
Material & Methods: Cross-sectional analytical study was conducted to examine the immunohistochemical expression ...
Rafa Almeer1,*, A. Alqarni1,2, S. Alqattan3, S. Abdi4,*, S. Alarifi1, Z.Hassan5 and A. Semlali6
...ed by MTT assay and gene expression of three tissue inhibitors of metalloproteinase (TIMPs) and two matrix metalloproteinase (MMPs) were measured by real-time PCR. All subgroups exhibited altered morphology with accelerated detachment compared to untreated cells. The MTT assay after 48 h revealed that treatment with H1 and H2, reduced cell viability by 48% and 91% respectively, compared to that of untreated cells. These results suggest that anticancer effect o...
Jingen Xu1, Yang Liu2, Erhui Jin1, Youfang Gu1, Qin Zhang3 and Shenghe Li1,*
...d that differential gene expression involved in biological processes, including the G-protein coupled receptor protein signaling pathway, intrinsic to membrane, cell junction and ion transport. KEGG pathway analysis showed that the neuroactive ligand-receptor interaction was significantly enriched. The results offer a foundation for exploration of immune functions and genetic control of peripheral blood T lymphocyte subsets. Also, several differentially expres...
Muhammad Khan1,*, Amara Maryam2, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1 and Yongming Li3,*
...stasis by modulating the expressions of p-STAT3, COX-2, MMP-2, MMP-9, E-cadherin and N-cadherin. Moreover, we found that chronic BaP exposure promoted migration without enhancing the proliferation of cells and BHS-I was equally toxic to both BaP-treated and untreated A549 cells. Taken together, the data of present study indicate that BHS-I not only inhibits metastasis but also induces cell death in BaP-treated A549 cells. Therefore, BHS-I may become a potentia...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...control groups. The mRNA expression and protein secretion of supernatant were tested using Q-PCR and ELISA method, respectively. Co-culture KM&FM under 3.4 kPa pressure enhanced typeIcollagen and type III collagen mRNA expression and protein secretion from KM. It however reduced typeIcollagen and type III collagen mRNA expression and protein secretion from FM; it promoted mRNA
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
.../9 protein synthesis and expression. So we concluded that Wumei Pill probably reduce the release of pro-inflammatory cytokines and the bacterial endotoxins by blocking the transmission of TLR4/9-NF-kB signaling pathway so that Wumei Pill has a significant therapeutic effect on IBS-D.
...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...led a bimodal pattern of expression with two maxima, first at 15 min and second at 90 min exposure time. The role of CueO in copper detoxification as well as its laccase activity makes it suitable for biotechnological applications.
...

Sher Nawab Khan* and Ghulam Hassan 

...aits which indicated the expression of additive gene effect for controlling the inheritance of these traits. 

...

Muhammad Babar Khawar, Nadeem Sheikh*

Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
...led an overall decreased expression of various proteins in both experimental groups. The protein fractions of 317, 212, 178, 134, 89, 74 and 68 KDa were found in Group 1 compared to control (331, 215, 180, 139, 112, 91, 75 and 69 KDa). However, protein fraction of 202, 171, 136 and 91 KDa were present in Group 2 compared to control. These findings confirmed that paper industry leachate is extremely noxious and induce alterations in various serological paramete...

 Saiqa Andleeb* , Shaukat Ali

RNA interference; A tool for treatment of human diseases
...que to knockout the gene expression by introducing either short interfering RNA or double stranded RNA fragments into targeted host. These RNA fragments degraded specific homologous mRNA. RNAi technology used as an essential tool in several model organisms such as invertebrates, mouse and fly to explore the role of individual gene. In recent era, RNAi is directly used in therapy of various infectious and non-infectious diseases. Variety of human diseases like ...
Shaher Bano1, Sarah Ghafoor1,* and Nadia Naseem2
...egulation of TGF-α expression during postnatal salivary gland development as it is currently unknown.Twelve male rats were taken at week three and twelve at week seven and divided into control and experimental groups. Control rats were given normal saline while experimental rats were given T3 at a dose of 1mg/kg body weight. All the animals were sacrificed on 15th day. Weight of each rat was calculated at the time of T3 administration. Body we...
Gangchun Xu1,2, Fukuan Du2, Yuyu Wang2, Yan Li2, Zhijuan Nie2 and Pao Xu1,2,*
...he spawning process, the expression of PTGES2a did not significantly change, remaining at a low level. In contrast, PTGES2b expression significantly increased during spawning. These results provide basic knowledge of the new PTGES2s of C. nasus.
...
Zhen-li Li1,2, Hui-fang Zhou2,*, Bo-ru Zhou2, Bei Liu2, Xiao-fei Jiang2 and Jian-ya Xu2

 

..., FSHβ and LHβ expression levels in the pituitary were determined by qPCR, and GnRHR expression levels were assessed by qPCR and western blot analysis. Simultaneously, transcription factors, such as c-Jun, Elk-1, Egr-1, Nur77, CREB, and transduction molecules in PKC-MAPK, Ca2+-CAM, and cAMP-PKA signaling pathways, such as PKC, p38mapk, ERK, CAM, and PKA were assessed by real-time PCR and western blot analysis. BSZY...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...t of hemodialysis on the expression of CXCL8 in serum and its mRNA in neutrophils of the patients with chronic renal failure (CRF). It was used to dynamically observe of Hemodialysis on the concentration of the CXCL8 in serum and the levels of CXCL8 mRNA in neutrophils were respectively measured by ELISA and PCR. The house keeping gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded ...
Bei Liu1, Yanli Hong1, Huifang Zhou1,*, Zhenzhen Cao1, Shuang Zhang1, Jing Jin1, Miao Jiang1, Cunsi Shen2 and Jianjian Ji2
...tly decreased, while the expression of GnRHR was significantly increased compared to the blank group (p<0.05). Use of CSF with BSZYD alone showed no significant effect on the secretion of RPC, and the expressions of GnRHR and related hub genes in cAMP-PKA pathway. However, it exerted an effect on the model-treated group. Further, no significant difference was found in the content of cAMP and the levels of mRNA and ...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...ion of circulating miRNA expression. Despite recent advancements, there is not a single consensus on the use of reference gene for quantification of circulating miRNAs through qPCR analyses in ALL. In the current study, we identified the reference gene that is the most suitable for qPCR normalization in patient and control plasma samples of ALL. Three highly reported reference genes namely RNU6-2, mir-16-5p and cel-mir-39-1 were selected as the candidat...

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

... It appears that disease expression was significantly influenced by pathogen inoculation method. The infection caused by S. scitamineum and subsequent whip smut development has also been greatly varied with the inoculation method. Injection method followed by wound+paste method and dipping method produced maximum disease incidence, highest number of whips, more tillering and less germination. While, other inoculation techniques, including soil infestation and ...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...at the liver ChREBP mRNA expression level was significantly positive or negative effect on serum total protein (TP), albumin (Alb), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) (P<0.05 or P<0.01). The g.247075G>A mutation was significantly association with these measured serum biochemical indexes except for LDL-C. Significant additive effects were detected for a...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...ub-cloned in pET28a. The expression of recombinant cellulase was analyzed using BL21 CodonPlus (DE3) cells as expression host. The expression studies resulted in the production of recombinant enzyme as soluble protein. The recombinant protein was purified by affinity column chromatography. The characterization studies of purified protein demonstrated the optimal enzyme activity at 90°C...
Luo Lei1, Xingxing Deng1, Dengyue Yuan2, Zonglin Zheng1, Chengke Zhu1, Hui Luo1, Baohai Li3, Hua Ye1,* and Chaowei Zhou1,3,*
...igated the effect of its expression at different nutritional levels. Herein, we found that gibel carp npy has an Open Reading Frame (ORF) containing 291 bp. Moreover, the expression of npy was detected in all tested tissues, with the hypothalamus showing the highest expression in gibel carp. The npy mRNA expression in the hypothalamu...

 Ellen Y. Zhang

...e employment of negative expressions in his philosophy. Zhuangzi’s claim that “The perfect happiness is derived from the absence of happiness” designates two key arguments: (1) Happiness cannot be designed and measured, and (2) There is no single fixed notion of happiness and meaning-making. In conclusion, I attempt to show that flow experiences, in the context of Zhuangzi’s Daoism, point to a fluid and creative mind that transcends the...

 Xu Lijie, Obaid Ullah, Liu Haixing, Ihsan Ali, Zhongshu Li* and Nan-Zhu Fang*

... genes and regulates the expression of genes that associate with NADPH oxidase. To understand the role of PPARγ in the development of early embryos and define the mechanism responsible for the arrest in development during the maternal-to-zygotic transition (MZT), we used an embryo model of oxidative damage by H2O2. We found that H2O2 exposure significantly decreased embryo development, increased the intracellul...

 Shaher Bano1, Nadia Naseem2 and Sarah Ghafoor1,*

...nd (SLG) development and expression of TGF-α. The comparison of TGF-α expression in SLG with that of its expression in SMG during post-natal rat salivary gland development has not been documented in the literature so far. Following ethical approval, a total of twelve rats were divided into two groups. Group A1 consisted of six animals taken at the age of four weeks and sacrific...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...trols to investigate the expression of miRNA panel. Plasma was isolated by standard protocol of centrifugation and then isolation of total RNA including miRNA was done. About 100ng of isolated miRNA was converted to cDNA after addition of poly (A) tailing and then expression profiling was done by using real time-qPCR technique in which miR-specific DNA primers were used to increase sensitivity of reaction.

 Tazeen Jamil, Saba Ijaz, Rabia Arif*, Faiza Akram and Muhammad Saleem

...ns leads to altered gene expression. We examined the diversity of retroelements in four consecutive generations of different strains of Sordaria fimicola. The parental strains werecollected from two contrasting environments of Evolution Canyon i.e. harsh South facing slope and neutral North facing slope. Retroelements profiles were generated in order to detect retrotransposons. Strains from South facing slope exhibited more copy number of Ty3-gyp...

Ihsan Ullah Khan*, Muhammad Arshad, Muhammad Ayub Khan, Muhammad Ashraf, Ashiq Saleem, Sundas Awan, Samra Azam and Shamim Ul Sibtain Shah 

...ct of heterosis on their expression. Comparatively higher and positive heterosis was exhibited by all hybrids for traits such as PHT (52.16-84.91 %), ST (2.83-191.85 %), HD (19.98-68.49 %) 100-GW (12.21-64.44 %) and SY (kg ha-1) (125.47-175.21 %) relative to parental average. The highest magnitude of heterosis for seed yield was exhibited by the cross CMS-77 x R-83(1) (175.21 %) relative to mid-parent values. The impact of mid-parent heterosis on the oil conte...

Qaizar Ahmed1, Fida Mohammad2, Sheraz Ahmed2*, Sultan Akbar Jadoon2, Imtiaz Ali2 and Ajmalud Din2 

...pe of gene action in the expression of all traits across all environments, except nicotine content. Contrarily, Hayman analysis identified both (additive and dominance) type of gene actions for yield and other traits. Similarly, Hayman analysis discriminated gene actions for various traits more specifically across environments. Based on this study, Griffing analysis was revealed to be more accommodative, whereas Hayman analysis was more stringent in specifying...
Aisha Khalid1, Muhammad Tayyab1,*, Abu Saeed Hashmi1, Tahir Yaqub2, Ali Raza Awan1, Muhammad Wasim1, Shagufta Saeed1, Sehrish Firyal1 and Abdul Rauf Shakoori3
...CodonPlus (DE3) cells as expression host and pET28a as expression vector. Effect of various concentration of Isopropyl β-D-1-thiogalactopyranoside (IPTG), post induction time, effect of temperature and pH were examined for the maximal production of recombinant cellulase. The effect of supplementation of LB medium with additional carbon and nitrogen sources was also analyzed for maximal production of recombinant protein....
Shengjie Zhou1,2, Pengfei Wang1,2, Chao Zhao1,2, Mingjun Fu3, Jian G. Qin4, Lihua Qiu1, Zhenhua Ma1, 4,* and Maoshang Lin1
... that the highest tissue expression of L-FABP gene was observed in fish liver on 18 DPH. Along with the ontogeny of fish larvae, the expression of L-FABP gene was low at hatching, and quickly increased with the increase of fish age from 0 DPH to 4 DPH, and reached the highest level on 12 DPH. The highest expression of L-FABP gene was observed in fish cultured at 29°...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...results showed many HDPs expressions were suppressed by thiram-induced TD, and the expressions of HDPs were upregulated by rGSTA3 protein. These findings demonstrated that mRNA expressions of HDPs are highly related to thiram-induced TD, and rGSTA3 protein enhanced the immune response of chicken erythrocytes by up-regulating HDPs expression. Future studi...

Abid Ali1*, Hidayat ur Rahman1, Farhatullah1 and Zahir Shah

...sible in controlling the expression of plant and ear height, maturity and stover yield as well. Additive x dominance type of epistasis played major role in the inheritance of ear leaf area, stover yield and harvest index in all crosses. Similarly, in the inheritance of days to silking, ear leaf area, ear length, stover yield, biological yield and harvest index additive epistasis was involved. Based on the findings from this study, crosses SW-491 × EV-157...
Hong Zhang1, Shu-Fen Han2, Jing Wang1, Shao-Kang Wang1, Gui-Ju Sun1 and  Cheng-Kai Zhai1, *
...in 1c (SREBP-1c) protein expression in rats’ tissues. Compound whole-grain ameliorates diet-induced obesity and hyperlipidemia by enhancing PPARγ and reducing SREBP-1c in rats. 
...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

...e embryo, including gene expression by the donor cells. The human hematopoietic stem/progenitor cell 117 (HSPC117) protein has been identified as being involved in placental formation, and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porc...

Muhammad Yasin1, Romana Shahzadi1, Muhammad Riaz2, Mahideen Afridi2, Wajya Ajmal3, Obaid Ur Rehman2, Nazia Rehman3, Ghulam Muhammad Ali1,3, Muhammad Ramzan Khan1,2,3* 

...ld losses in canola. The expression of SHATTERPROOF1/2 (SHP1and SHP2) and FRUITFULL (FUL) MADS-box genes is fundamental to fruit dehiscence zone and valve margin, respectively. The present study was envisaged to isolate the orthologs of SHPs and FUL from local canola “Pakola” and “Punjab Sarsoon 3”, and to study expression patterns of their transcripts. Morphological data revealed significant differen...
Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1
...s aimed at analyzing the expression levels of genes that are induced in response to oxidative stress in heart and liver tissues following DOX administration to rats. In this study, DOX was administered to rats intraperitoneally (i. p.) at 3 mg/kg at alternate days for two weeks. Heart and liver tissues were harvested and the mRNA levels of NAD(P)H quinone dehydrogenase 1 (Nqo1), glutathione peroxidase (Gpx1) and isocitrate dehydrogenase-1 (Idh1) were analyzed ...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...2, and Cyclin D1 protein expression in peripheral blood.The red blood cell count and hemoglobin levels decreased in 5-Fu group compared with the control group (P < 0.05). 5-Fu+APs group showed a marked increase in the number of erythrocytes and a significant increase in hemoglobin content (P < 0.05). Compared with the control group, 5-Fu group showed a significant decreased in the number of CD71/Ter119-labeled erythrocytes (P < 0....
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
... proliferation, NaPi-IIb expression, and the absorption of phosphorus in porcine intestinal epithelial cells (IPEC-1). The experiment consisted of 1 control treatment and 5 levels of CT treatments (1×10-12 to 1×10-8 mol/L), and each treatment has 6 replications. Cell proliferation was determined by the MTT method. Cell morphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The...
Hafiz Suboor ul Hassan and Saira Malik*
... occur in adulthood when expression of the lactase gene diminishes. Two SNP’s LCT-13910C/T and LCT-22018G/A are considered to be associated with the development of lactose intolerance in humans. The aim of the study was to evaluate the role of LCT-13910C/T and LCT-22018G/A with the development of lactose intolerance in Pakistani population. A total of 160 samples were collected and tetra primer ARMS-PCR was used for genotyping. 79.48% lactose intolerant ...
Kun Wang1,2, Yinglin Cui2, Xu Zhao2 and Changjiang Hu1*
... immunofluorescence, the expression of claudin-5, ZO-1 and VE-cadherin was detected. The expression of DNA methyltransferase 3b (DNMT3b) and Matrix metalloproteinase-9 (MMP-9) was evaluated by western blot. And microRNA-29b was identified using quantitative real-time PCR. 3). Compared with the Model group, the group treated with XN had a significantly reduced number of dead neurons in hippocampus and cortical regions of ICH ...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...r pTG19 and subcloned in expression vector pET21a. E2 protein, expressed in insoluble form, was purified by repeated sonications, followed by denaturation and refolding through fractional dialysis with urea. Multiple alignment with other sequences showed nucleotide variations of HCV3a E2 compared with already reported sequences. A total of 140 (11%) point mutations were found in Pakistan’s gene sequence, out of which general and specific differences were...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
... was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family proteins modulation. Finally, piceatannol significantly enhanced apoptotic efficacy of CDDP in U2OS cells. On the basis of our findings, piceatannol is a promising anticancer agent which could be developed int...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...analyzed, and its’ expression pattern was further determined. It consists of 1185 nucleotides that encodes 394 peptides bond. The BMP15 gene was observed to be expressed in cumulus tissue, granular tissue, hypophysis, genital ridge and tissues of ovary and testis. Moreover, QRT-PCR results showed that BMP15 was expressed in the whole process of embryogenesis and folliculogenesis, early high level and then down regulated. It was significantl...
Yue Ren1, Peng-Fei Liu2, Wan-Long Zhu1,*,Hao Zhang1 and Jin-Hong Cai1
...ulated transcript (CART) expressions were measured. The results showed that body mass and serum leptin levels were lower significantly in winter than that of in summer in five areas. But thermogenic characteristics and food intake were higher significantly in winter than that of in summer in five areas. NPY and AgRP expressions showed significant differences between two seasons, which were higher in winter and lower in summe...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

.... MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradiation-induced TGF-β1 production and ameliorate collagen deposition by inhibiting NF-κB s...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...o specifically knockdown expression levels of the endogenous gene ABCA1 in bovine mammary epithelial cells (BMECs) to analyse the effect on intracellular triglyceride (TG) content by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, cell TG Assay and RT2 Profiler PCR array. The results showed that ABCA1 knockdown reduced the TG content in BMECs. On the other hand, the RT2 Profiler PCR array demonstrated that the ...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

...e, and imbalances of its expression may cause liver tissue damage. At present, few studies on CYP1A1 methylation and anti-tuberculosis drug-induced liver injury are available. Studying the relationship between CpG island methylation in the CYP1A1 promoter region and drug-induced liver injury is, therefore, of great importance. Anti-tuberculosis drugs induced hepatotoxicity in HL-7702 cells was investigated. Dehydrogenase activity was detected by a lactate dehy...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in MPP children were (298.917±51.860) pg/mL and (1.848±0.525) lgcDNA/lgGAPDH. There were significant differences between the trial groups and normal controls (P<0.05). Further observation showed that the levels of CXCL8 mRNA in peripheral blood of the children with severe illness were significantly higher than those in light cases (P<0.05). Intravenous infusion of Eryt...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...showed that the secreted expression of rhP4H was achieved successfully in P. pastoris GS115 cells. The catalytic activity of rhP4H was tested in a system that contained O2, Fe2+, α-ketoglutaric acid and ascorbic acid. Liquid chromatography coupled with tandem mass spectrometry was used to monitor the hydroxylation of proline. The results showed that rhP4H specifically hydroxylated proline residues only in the Gly-X-Y structur...
Memis Ozdemir
...ecretion of milk and the expression of milk protein genes. It has been seen many studies about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymor...

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

... into three types of overexpression constructs i.e. pBinAR_NtSAT4 (targeted to cytosol), pBinAR-TKTP_NtSAT4 (targeted to plastids) and pBinAR-SHMT_NtSAT4 (targeted to mitochondria). For stable transformation of B. napus floral-dip and tissue culture based approaches were tested using different formulations of phytohormones for calli, shoots and roots induction in a variety of genotypes. While false positive lines were obtained in the result of floral-dip metho...
Hafiza Sadaf Zahra1, Asia Iqbal2, Sayyeda Hira Hassan1, Hafiz Abdullah Shakir1*, Muhammad Khan1*, Muhammad Irfan3, Chaman Ara1, Shaukat Ali4
...tween ALAN and oncogenes expression through epigenetic remodeling by DNA methylation and histone acetylation.
...
Mehwish Faheem1*, Maleeha Rafeeq1, Aisha Majeed1 and Saba Khaliq2
...phenol A caused decrease expression of cyp1a1 in a concentration dependent manner. Exposure to graded concentrations of bisphenol-A resulted in significant increase in mRNA expression of heat shock proteins (hsp70 and hsp90). In the light of present results, expression of cyp1a1 or heat shock proteins may be used as biomarkers to assess the presence of BPA in th...
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslamand  Samina Jam Nazeer Ahmad1, 2
... and KPK population. The expression of P450 gene through PCR showed enhanced level of P450 gene in different Instars as well as collected population. The dsRNA specific for CYP450 gene was designed and targeted through dsRNA in thoracic region of R. ferruginous through micro-injection. Gene expression and proteomic study confirmed the down regulation of P450 gene and associated protein in dsRNA treated populati...
Saman Muhsin Abdulkareem* and Nadir Mustafa Nanakali
...enzymes activity and the expression of cytochrome P450 1A1 (CYP1A1) against hepatotoxicity induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in male rat. Thirty adult male Wistar rats randomly divided into five equal groups. TCDD and QCT were orally administered at the dose of 10µg/kg /day and 20 mg/kg/day, respectively by gavage dissolved in corn oil for 90 days. At the end of the study, animals were sacrificed and their liver were removed...
Anam Tariq, Alina Gul, Majida Atta Muhammad, Samia Falak and Naeem Rashid*
...i. Heterologous gene expression resulted in production of recombinant protein in the cytoplasm which secreted gradually to the extracellular culture medium. Determination of the N-terminal amino acid sequence of the recombinant protein, in the extracellular medium, revealed that the 19 amino acid signal peptide was cleaved between Ala19 and Gly20. It seems probable that the signal peptide of TK0522 can be used for secretion of other r...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...regulate downstream gene expression. Single nucleotide polymorphisms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study,...
Chuntao Li1, Hua Liu2, Jianqing Zhang1*, Jiaqiang Zhang1, Luming Dai1, Zhihuan Zhao1,Lizhou Fang1, Ling Liu1, Jingkui Shu1 and Jiagang Feng1
...We detected differential expression of mRNAs and lncRNAs in blood samples from COPD patients (n=3) and healthy controls (n=3) with smoking history by human microarray analysis. Then, we selected lncRNAs and their associated mRNAs which were both differential expression in COPD vs healthy smokers, for quantitative reverse transcription PCR (qRT-PCR). A total of 3,359 lncRNAs revealed by microarray analysishave been differenti...
Mingsan Miao*, Hui Zhao, Jiaojiao Jia, Xiaoyan Fang and Yanyan Miao
...tissue by inhibiting the expression of inflammatory factors, repairing damaged nerve cells and improving the pathological changes of cerebral ischemia, proposing its therapeutic potential in treating such anomalies in future. 
...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...s to detect the relative expression level of Multi-drug resistance gene (MDR1) in common tumor malignancies, and evaluate the prognostic value of MDR1 expression in common malignancies. mRNA relative expression levels of MDR1 were detected byreal-time quantitative polymerase chain reaction (RT-qPCR) in tumor, adjacent, and non-cancerous tissues. The tumor markers were detected with COBAS 6...
Wenping Hu1, Xiangyu Wang1, Xiaodi Sun2, Ran Di1, Qiuyue Liu1, Zhangyuan Pan1, Xiaofei Guo1, Xiaohan Cao1, Jinyu Wang2, Yingjie Zhang3, Mei Jin4, Yuze Yang5 and Mingxing Chu1*
...t the association of the expression and polymorphism of TAC3 gene with the estrus of sheep. TAC3 expression in uterus and pituitary gland increased in estrus of Small Tail Han sheep and Tan sheep. The TAC3 expression level in uterus might relate with estrus of sheep. Two mutations of TAC3 were found in intron 1 (C327T) and exon 2 (G1803A) separately. Although th...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...ed by a maximum level of expression of pan cytokeratin – 299 ± 0.6, actin – 299 ± 0,58, vimentin – 265,7 ± 20,7, E-cadherin – 298,3 ± 1 points, indicating that this cells have a high adhesive properties, proliferation activity, cell signaling, interaction and mobility. The level of expression of these proteins remains high in the stem cells culture also at the Xth
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...e for one week, the mRNA expression levels of mucosal Mucin-1, Mucin-2, regenerating islet-derived protein 3 gamma (Reg3γ), and tumor necrosis factor (TNF) were significantly downregulated. The mRNA expression levels of mucosal β-defensin, regenerating islet-derived protein 3 alpha (Reg3a), regenerating islet-derived protein 3 beta (Reg3β) and secretory immunoglobulin A (sIgA) levels were significantly increa...
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
...group. Additionally, the expression of antioxidant enzyme genes including nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), SOD, and glutathione peroxidase (GPx) were improved by FR supplemented into the broiler diets. Based on these results, FR could be acted as a beneficial feed additive with antioxidant capacity and meat quality-improving effect in broiler diets.
...
Masroor Ellahi Babar*
...yed critical role in the expression of milk protein. It stabilizes the milk micelle and gene 5’ flanking region which works valuably in transcription regulation. Current study illustrates that Kappa-casein gene 5︠ flanking region in Dromedary camel of Pakistan is highly polymorphic and it is phylogeneticaly linked with other mammals. The analyses of the sequence of 5︠ flanking region in various breeds reveals the presence of different polymorphic reg...
Weihao Chen1,2, Zhilong Tian2, Lin Ma2, Shangquan Gan3, Wei Sun1,4,* and Mingxing Chu2,*
...>To elucidate the tissue expression levels of BMPR1B, BMP15, GDF9, Smad1, Smad5, and Smad9 genes in rams with different fecundity, quantitative real-time polymerase chain reaction was used to investigate the expression level of six genes in the brain, cerebellum, hypothalamus, pituitary, testis, epididymis, vas deferens, and adrenal gland in high fecundity (Small Tail Han sheep) and ...

Jie Yang

...ot, the mRNA and protein expression were detected, respectively. Cellular apoptosis were examined by flow cytometry and Western blots was applied to assess the cleaved caspase-3, -8, -9, and cleaved poly-ADP-ribose polymerase. Protein expression of extracellular-regulated kinase were detected. Finally, data analysed statistically to determine significantly differences among groups. Results showed that breakpoint cluster 2 wa...

Gulnaz Hameed1, Abdul Saboor1, Khuram Nawaz Sadozai2*, Ghaffar Ali2, Dawood Jan2 and Mansoor Rasheed

...to model the probability expressions with the help of Logit to investigate the correlates of poverty in the research area. Poverty was found at below for registered members and investigations showed that education, area under cultivation, possession of assets, status of employment, ownership of livestock and jobs of females increased the likelihood of decrease in poverty. Therefore, successful components of this program should be replicated with great care and...
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
... to detect Bax and Bcl-2 expression. The protein levels of TGF-β/ Smad / ERK signaling pathway were detected by Western blot. We found that PAP significantly increased cell viability after DOX-induced injury, reduced LDH, CK-MB levels, up-regulated Bcl-2 expression and down-regulated Bax expression levels. In addition, PAP can delay cell G2/M phase arrest, reduce apoptosis, significan...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...haracteristics, and gene expression, thus providing a theoretical basis for the genetic manipulation of A. cinerarius. Illumina RNA-Seq was used to perform massive sequencing of 18 cDNA samples derived from male adults, female adults, and pupa of A. cinerarius in southern and northern Xinjiang. We extracted total RNA from 18 samples and synthesized cDNA via reverse transcription. Then, Illumina sequencing was performed and unigenes were identifie...
Yuhong Li1, Dongxue Wu1, Xue Wang1, Mi Zhang1, Hanyu Zhu1, Zhe Shi1 and Fumin Feng1,2*
...pa;B) regulates the gene expression of eukaryotic cells. This study aims to reveal the changes in NF-κB during the development and progression of liver injury induced by anti-tuberculosis drugs in mice. A total of 168 Kunming mice were randomly divided into four experimental groups and one baseline group. The drug groups were orally administered with isoniazid (H), rifampicin (R), pyrazinamide (Z), and combination of these three drugs (HRZ) at 3, 5, 7, 1...
Yue Qin Song, Hui Zhong Sun* and Jun Feng Dong
...g and sequencing. Tissue expression profiles of all YsigOR genes in the antennae of females and males were analyzed using real-time quantitative PCR (RT-qPCR). The result showed that some YsigOR genes displayed significant differences in the expression levels between sexes. YsigOrco had the highest expression level in all YsigOR genes; however, the expression

Anila Latif, Zaheer Abbas, Farhatullah and Ghulam Muhammad Ali* 

...ed through PCR while the expression was confirmed by Reverse Transcriptase PCR. Whole plant bioassay confirmed 100% mortality in DBM within 72 hours. The present investigation concludes that the metabolic engineering of berberine in plants is an effective strategy to protect plants from herbivorous insects and this pathway should further be evaluated for possible application in agriculture. 

...

Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*

... the PiggyBac eukaryotic expression vector, which was stably expressed in Sertoli cells, thus establishing a PB-GDNF-Sertoli cell line. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that the GDNF gene expression in the PB-GDNF-Sertoli cell line was significantly increased compared with that in the control Sertoli cell line (p<0.01). Enzyme-linked immunosorbent assay (ELISA) results showed that ...

Muhammad Medrar Hussain*, Asad Jan* and Sayyar Khan Kazi 

...onfirmed that due to overexpression of OsTZF8 gene, transgenic line A and B displayed considerable level of drought stress tolerance. Additionally, the chlorophyll content of top four leaves of OsTZF8-OX transgenic indica line A and B were observed to be high compared to control. In conclusion our study revealed that over-expression of OsTZF8 gene has significant role in conferring tolerance to drought stress in rice. <...

Sarhad Journal of Agriculture

June

Vol. 36, Iss. 2, Pages 374-733

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