Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2
Email: y.lhor@yahoo.fr
...uetongue virus (BTV) and identification of the main breeding sites of Culicoides. From April 2009 to March 2010, the study was carried out at 14 sites distributed across the country. Results indicated that the two most abundant Culicoides species were Culicoides imicola (94.2-95.85%) and Culicoides newsteidi (2.21-2.72%). The rate of prevalence of these species was bi-phasic and higher from April to June, and October to November 2009. Inter...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: kohlc@rki.de
...l specimens promises the identification of novel, yet unknown, infectious diseases and etiologies....
Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2
...s calculated for problem identification. It was found that machinery was provided to almost half of the respondents. Similarly seed was the most provided input followed by fertilizers. Top most source of agriculture information was input dealers followed by Model Farm Services Center. The top learned skills by respondents in MFSC were the agronomic practices whereas there was big gap in horticultural practices. Major constraints identified were less machinery ...
Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
...tify;">Efficient identification of viruses is paramount to not only define the cause of uncharacterized infections but also to estimate the dynamics of viral population in the infected hosts. Several recent methodologies and chemistries have been proposed for viral diagnostics with variable outcomes and successes. In this review, we discuss a novel approach to identify the least transcripts of viral genomes using suppression subtractive hybridization t...
Gul Naz and Mohammad Akmal
...ar. The suitable variety identification for agro-climate and its optimum N-fertilizer demand with application are need of the day to overcome on N-leaching from the soil and to ensure future food security. Present study was, therefore, conducted in two seasons (2011-12 and 2012-13) at the Agronomy Research Farm, the University of Agriculture Peshawar, Pakistan. Treatments were laid-out in a randomized complete block design; split plot arrangements using four r...
Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker
Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa
... isolation and molecular identification of Mmm from the ear canal of cattle in Nigeria. It is recommended that samples from the ear canal be considered when Mmm isolation is intended so as to improve the chance of recovery. ...
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
Haji Muhammad, Zafar Iqbal* and Saira Saleemi
...variety of fish nets and identifications of these fishes were made by using standard taxanomic keys on the basis of morphometric characters. Seventy species belonging to 43 genera and 17 families were recorded from the study area of Indus River. Thirty two species were commercially important. Alpha Diversity Indices study showed that fish diversity of the River was quite rich (species richness, 70) and diverse, (Shannon’s index=3.66), (Simpson-D=0.96), E...
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
... HH. This study aimed at identification of mutations in GPR54 and GNRHR genes and their correlation with HH in Pakistani boys. Thirty one boys with delayed puberty and thirty one normal age matched controls were examined. Genomic DNA was extracted and amplified by PCR using specific primers for GPR54 and GNRHR splice site exons. Mutations were analyzed by single-stranded conformation polymorphism (SSCP) and/or sequencing. No mutation was identified in GPR54 ge...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...n genotyping and genetic identification is of essential significance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP...
Jose M. Rojas, Noemi Sevilla and Veronica Martin
...has been reported in the identification of viral factors involved in the host immunosuppression, although future studies employing in vivo infection models are needed to clearly understand the mechanisms developed by these viruses, in particular PPRV, to interact with the host immune system. A better comprehension of this behaviour could lead the global strategy for the control and eradicaton of PPR, initiated by Food and Agriculture Organization of the United...
John G. Bruno
... if coupled with genetic identification techniques such as PCR or gene probe hybridization....
Muhammad Saeed* and Iftikhar Hussain Khalil
...tive of the research was identification and proper selection of best performing wheat parental genotypes and best F1 crosses, based on GCA and SCA estimates. Significant differences were observed among the wheat genotypes for all the studied traits. The estimates of σ2gca and σ2sca and its ratio (σ2gca/σ2sca) indicated that non-additive genetic expression were pre-dominant for most of traits studied. Line BRF3 and testers ZM04 were good...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahim2 and Ghada Kamal El Khalafawy2,*
...t-align: justify;">Early identification of yeast to the genus and species level is necessary for effective antifungal therapy, and can also facilitate control of infections. A total of 50 isolates were isolated from 60 swab samples collected from two poultry processing plants represented broiler carcasses swabs and worker (hand swabs and nail scrapings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consumin...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...y for future unambiguous identification using an integrative taxonomic approach that combines morphology and molecular analysis of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Adult P. websteri exhibit a high degree of morphological plasticity in the palp pigmentation pattern, the shape of the anterior edge of the prostomium, the shape of the major spines on chaetiger 5, and the shape of the pygidium. The COI gene sequence demonstrated t...
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
...study focused on a rapid identification of dengue virus from larvae of Aedes aegypti (L) and Aedes albopictus (Skuse) by using PCR. During this study, larvae of A. aegypti and A. albopictus were collected from different localities of Lahore during May to September of 2012 to 2014. RNA extracted from each mosquito larvae pool was tested by nested PCR for detection of dengue virus. Out of total 50 pools of Aedes aegypti larvae,...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...vely high. Morphological identification of G. expalanatum was carried out on the basis of size and shape of worm. The hematological and bile biochemical changes were found in two groups. Pathologically significant changes were observed in the infected tissue. The parasites had formed pocked in the bile duct mucosa, glandular hyperplasia, thickened blood vessels. The results demonstrate that morphological markers, pathological, hematological and bile bio...
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
... compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. using brain heart infusion broth supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples were subjected to genomic DNA extraction to detect Mycoplasma spp. using mult...
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...tify;">The detection and identification of viruses has been a challenge since the advent of the discipline of plant virology over a century ago.Agreat variety of methods have been developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subsequently by Enzym...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...e help of BLAST for SNPs identification. This is a first report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorphisms (SNPs) were reported. Heterozygosity, allelic and genotypic frequencies of identified variants were also determined. Chi2 test was performed to evaluate the Hardy-Weinberg Equilibrium...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...dentical, their specific identification relies exclusively on the use of molecular tools. In this study, we analysed the G-protein-coupled chemokine receptor (GPCR) genes of two LSDV isolates from Ixodid (hard) ticks (Amblyomma hebraeum) in Egypt. Multiple alignments of the nucleotide sequences revealed that both isolates had nine nucleotide mutations in comparison with the local reference strain, LSDV-Egypt/89 Ismalia. Compared with the GPCR sequences of SPV ...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
Muhammad Zafarullah Khan
...n competencies regarding identification of input supply dealers’ malpractices and evaluation of pesticide companies activities. Timely appointment and delegation of authorities to subordinate staff were the top two utmost possessed competencies to perform efficiently and effectively of their assigned responsibilities followed by the third top ranked competency of helping field assistants to plan extension activities. The lowest competencies of AEOs repor...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
... molecular diagnosis. Theidentification of the same variant in CTSK as identified in other families from Pakistan suggests that it is common due to a founder effect.
...
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
...dy deals with isolation, identification and characterization of some indigenous heavy metal resistant bacteria isolated from rhizosphere of Tagetes minuta, growing in soil adjoining automobile workshops to investigate their bioaccumulation capacity for three selected metals Cr(VI), Ni(II) and Cd(II). The plant was selected due to its natural abundance in the climate of Kashmir and also in the area of study. On the basis of morphological, biochemical, 16...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
...ective DNA barcoding for identification.
...
Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*
H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2
...tudies for isolation and identification of Brucella organisms. Brucella melitensis biovar 3 could be recovered from 64 (61.5%) tissue specimens and 28 (60.9%) milk samples. Brucella cultures were further identified on molecular basis using universal and Bruce ladder PCR. ...
Sajid Muhammad* and Fida Mohammad
Ikramul Haq1, Aneela Zameer Durrani*1, Muhammad Sarwar Khan1, Muhammad Hassan Mushtaq2, Imtiaz Ahmad3, Amjad Khan2 and Mehboob Ali1
Syed Kashif Nawaz*, Faiza Zubair and Sidra Kanwal
... NCBI map viewer for the identification of exact loci of overlapping genes. It was noticed that maximum number (635) of overlapping genes were present on chromosome 1. Chromosome 21 carried the minimum number (85) of overlapping genes. Overlapping genes were further categorized on the basis of their orientation. It was found that the human genome has 62% embedded genes, 17% convergent genes, 16% divergent genes and 5% uni-directional genes. These categories ma...
RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
Tania Ahmed Shakoori1,*, Muhammad Saqib Saeed2, Asif Hanif2, Saima Mukhtar3 and Ayesha Ghauri4
Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain
...or bacterial growth. For identification of the characteristic bacterial colonies Cmm-specific primers CMM-5 and CMM-6 were used for amplification of 614bp product from pat-1 gene of bacterial plasmid. The whole bacterial DNA was used as template, extracted with standard procedures using commercially available kit i.e. protinase-K (sigma). 27 out of 34 isolates were confirmed as Cmm having 614bp band. Black nightshade (Solanum nigrum L) was the only weed from w...
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...lternative to phenotypic identification in a routine diagnostic laboratory. High diversity values were found in the studied area implying high population diversity of M. tuberculosis that’s being prevalent in Khyber Pakhtunkhwa, Pakistan.
... 
Amir Khatam*, Sher Muhammad and Ijaz Ashraf**
ROLE OF FARMER FIELD SCHOOLS IN ENHANCING SKILLS OF FARMING COMMUNITY IN KHYBER PAKHTUNKHWA, PAKISTAN
...the area of insect pests identification was ranked st 1 with mean value 3.22 closely followed by insect pests control by local nd rd recipes and their mass killing which were ranked 2 and 3 with mean values 3.03 and 2.84, respectively. Likewise, chemical and manual weed st nd control measures were ranked 1 and 2 with mean values 2.99 and 2.97, respectively. Correspondingly, farmers' skills in furrow irrigation was ranked at the top with mean value 3.32 followe...
Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari*
ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...tic diversity and hybrid identification. Out of the 26 RAPD primers used for DNA amplification, 14 primers produced polymorphic bands, while 12 produced monomorphic bands. These 14 primers yielded total 96 RAPD markers, out of which only 40 were found polymorphic which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was observed. On the whole, 87.50% g...
Shakir Ullah*, Aish Muhammad*, Iqbal Hussian*, Hafeez-Ur-Rahman**, Muhammad Zeeshan Hyder***, Muhammad Din**** and Nizamud Din
MORPHOLOGICAL VARIATIONS IN APRICOT CULTIVARS GROWN IN GILGIT BALTISTAN PAKISTAN
... study may be useful for identification and morphological variations of
apricot in Pakistan.
...
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...could be applied for the identification of various enzymes on routine basis directly in bacterial cultures and biological fluids.
...
Archana Naithani, Pongthep Suwanwaree* and Bartosz Nadolski
...rovince, resulted in the identification of 94 bird species belonging to 12 orders and 35 families. Altogether 3,328 individuals were observed. Out of 35 Families, Sturnidae and Sylviidae hadthe highest number of species (5 species each).Six simple dietary guilds were observed during the present study; the maximum number of species belonged to insectivore group and minimum to nectarivore. Analysis of data revealed that the average of species diversity index of ...
Mahwish Rehman1*, Sajjad Ahmad1, Maqsood Shah1 and Inamullah Khan2
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli isolates carrie...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...along with the molecular identification by 16S rRNA gene sequencing confirmed that caries isolates are pathogenic S. mutans and soil isolates belong to the genus Streptomyces. The antibiotic resistance profile of S. mutans illustrated resistance to optochin. The antimicrobial screening of Streptomyces against S. mutans showed that these strain are the prolific producers of active agents to inhibit the growth of caries causing...
Muhammad Shahbaz1,*, Muhammad Anwar Iqbal1, Arshad Javid2, Abu ul Hassan Faiz1, Irfan3 and Muhammad Irshad Arshad4
...s that help in clear cut identification of various Pipistrellus species. The external body, cranial and bacular features of the species were compared with available literature.
... 
