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Johanna Lindahl 1, 2

British Journal of Virology
...ngue virus is one of the pathogens benefitting from this. The related virus Japanese encephalitis virus is the most important cause of mosquito-borne encephalitis in the world, and is maintained in avian reservoirs and amplified by pigs. The occasional spill-over of the virus into humans results in more than 60 000 clinical cases of Japanese encephalitis annually, with high case fatality rates. The main vectors preferably feed on livestock and breed in rice f...

Lei Chen, Ling Zhu, Zhi-Wen Xu, Wan-Zhu Guo

 

Molecular Genetics of Aichivirus C (Porcine Kobuvirus) in China
...hought to be a potential pathogeny of pig diarrhea. The review represented the discovery of Aichivirus C in China, and made a brief summary about molecular and epidemiology characterizations of Chinese Aichivirus C strains. Mutiple Aichivirus C strains and Aichivirus C variants are circulating in China. Recombination events were also observed in Chinese Aichivirus C strains. More further studies are needed to clarify the evolutionary features and

Xingdong Yang and Lijuan Yuan

...ses caused by a specific pathogen in the absence of interfering maternal antibodies, other maternal immune regulators, and intestinal and environmental microbes. Due to the similarities between pigs and humans in terms of genetics, physiology, intestinal anatomy, and immune system, pig is particularly a suitable animal species for the modeling of human enteric pathogen infections, immune responses and for vaccine evaluations...

Suresh V Kuchipudi* and Kin-Chow Chang

 

...mmune response and virus pathogenesis are increasingly recognized. Recent evidence strongly indicated that skeletal muscle cells fully support productive replication of influenza A virus (IAV) and could play an important role in disease outcome. We are of the opinion that skeletal muscle could be a key innate immune tissue/organ in the pathogenesis of influenza and other systemic viral infections which warrants further in-de...

Hu Zenglei1 and Xiufan Liu1, 2*

 

...justify; ">Virulence and pathogenicity of Newcastle disease virus (NDV) is one of the major determinants for the clinicopathological manifestations in infected hosts. Recent studies showed that the host innate immune responses to infection play important role in the pathologenesis of NDV, and correlate with the severity of the clinical disease. Virulent strains of different genotypes induce distinct pathological manifestations in chicken organs, especially in ...

Leonard Izzard1, 2, John Stambas1, 2*

...being used to manipulate pathogenicity and immunity and as biomarkers for disease severity.

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Shen Yang1, Guang-Zhi Tong1, 2*

... life cycles. The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is a major concern for the swine industry and causes high degrees of morbidity and mortality. The intracellular signalling cascades of the innate immune system triggered by HP-PRRSV often lead to the elimination of infection. However, HP-PRRSV induces pathological changes and can cause aberrant immune responses in the host, resulting in acute lung injury and immu...

Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*

E-mail | shizixue@gmail.com

...EV would remain a global pathogen and might cause worldwide pandemics. Currently, more research is required to be applied to JEV pathogenesis and antivirus therapy. This review aims to reveal the exigency of developing a worldwide effort to acknowledge the importance of performing an extensive study of this deadly disease.

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Brittany D. Rife 1,2, Marco Salemi 1,2*

E-mail | salemi@pathology.ufl.edu

...al dissemination of pathogens. Infection of a single host can be viewed similarly as an epidemic spread on a microscale, creating a unique opportunity for the application of phylogeography at the intra-host level, which has until recently been limited by dataset quality and the lack of a rigorous statistical framework. As recently described in studies using the SIV-macaque model of neuroAIDS, the incorporation of
spatial, temporal, a...

Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3

Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg

...bat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different condi...

Claudia Kohl*, Andreas Nitsche, Andreas Kurth

Email: kohlc@rki.de

...community of etiological pathogens, without any knowledge on the targets in advance. However, clinical specimens usually comprise an overwhelming amount of host nucleic acids, which by far exceeds the number of pathogen nucleic acids in the sample. Subsequently, it is necessary to either decrease the amount of host nucleic acids or increase the amount of pathogen nucleic acids, to allow fo...

Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*

...V) is generally a noncytopathogenic virus in cell cultures. The development of reporter CSFV has provided an excellent option to directly detect viral replication without the use of secondary labeling. This mini-review discusses the applications of reporter CSFV in the study of CSFV biology, including efficiently screening the antivirals against CSFV and viral receptors, conveniently tracking the viral proteins in live cells as well as improving diagnostic met...

Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4

...gs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role the...

Moustafa Kardjadj1, Pam Dachung Luka2

...onsidered as an emerging pathogen.. However, the increased detection of the virus in the light of rinderpest eradication and the lack of awareness of the disease in areas where the virus has been detected for the first time in recent years given the educational status of farmers contributed to this emergence.

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Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4

...us and its host-specific pathogenesis. Moreover, understanding the nature of such circulating field viruses is essential to underpin the endemic potential of PPRV and its possible spread to the susceptible wild or domestic small ruminants.

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Neha Singh and Anjana Pandey

...erous cells, detect live pathogenic organisms, and to discover novel disease biomarkers to facilitate early detection of the disease and for therapeutic purposes.

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 John G. Bruno and Jeffery C. Sivils

...e surfaces of general nonpathogenic Escherichia coli (ATCC No. 8739) as well as alpha and gamma intimins and Shiga-like toxin 2 (SLT-2) B subunit verified that the aptamers bound proteins of the correct molecular weights for intact outer membrane proteins (OMPs), intimins and SLT-2. Some bands on the aptamer Western blots were shared between the “Big 6” non-O157 Shiga toxin-producing E. coli (STEC), E. coli O157 and related Gram negative bacteria. ...

 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

...and application of entomopathogenic nematodes (EPN) depend on the use of host insects for in vivo production. As there is little information regarding relationship between nematode dosage and production of infective juveniles (IJ) of Steinernema feltiae and ability to locate hosts, therefore, in the present studies investigations were done on these aspects. A significantly greater emergence of IJ of S. feltiae from Galleria mellonella was observed with White t...

John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure

...e a slum household-based pathogenic Leptospira population dynamics simulation model. The model will be analyzed to assess the role of slum-based biotic (human and animal) and abiotic (soil, water and weather) habitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have designed a pseudo-longitudinal survey to be undertaken for two years in Kibera slums in Nairobi city Kenya. Human and anima...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...malgam of beneficial and pathogenic microbes and its pH (6.7 to 7.4), high concentration of phosphorus (4.50% and 4.33%) and nitrogen (3.26% and 2.37%) favor seed germination, enhance root growth and soil fertility.

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Giselle R.R. Ayres* and Brandão P.E

...ription, replication and pathogenesis. The last 3’ one-third of the genome codes the four structural and accessory proteins. Nsps act in a complex replication process, made possible by the special characteristics of AvCoV genome. This manuscript aims to present an overview of the main aspects of the current knowledge on the main AvCoV replicase genes.

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Muhammad Raheel1,*, Nazir Javed2, Sajid Aleem Khan2, Hafiz Muhammad Aatif3 and Sohail Ahmed4

... exotic species of entomopathogenic nematodes (EPNs) was checked on Galleria mellonella larvae. The native species included Steinernema asiaticum and Heterorhabditis indica whereas exotic species were S. feltiae and H. bacteriophora. G. mellonella larvae were exposed to 300 IJs of each species. After inoculation at different temperatures, the reproductive potential of EPNs increased with increasing temperature and was found to be the best at 25oC. No EPNs spec...

John G. Bruno

...riching specific E. coli pathogens during aptamer-magnetic separation in food samples, leading to a greater ability to detect these serotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization.

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Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan

...ccount the genotypic and pathogenic characterization of H9N2 in avian species. However little is known about the impact of different strains on the innate immune response. In the present study, using quantitative real-time PCR, cytokines gene expression were examined in response to infection with two strains of Egyptian H9N2 (namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains revea...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

... role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A total of 400 individuals including age and gender matched healthy individuals (control group) and RA patients were genotyped. Detection of rs2070424 polymorphism was carried out using...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...ned against Wilt causing pathogens using the dual culture assay. Among them, Aspergillus flavus was found active fungus against the selected vascular Wilt causing fungus and bacteria, i.e. Fusarium oxysporum (87.50 ± 0.11% inhibition), Xanthomonas campestris (67.00 ± 0.46% inhibition) and Clavibacter michiganensis (72.00 ± 1.02% inhibition). The A. flavus was grown on two different media, i.e. Potato Dextrose Broth (PDB) and Glucose Nutrie...
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
...d isolates. Additionally pathogenicity of isolated Fusarium species was evaluated against nymph and adult of Bemisia tabaci. Under controlled conditions different species of Fusarium restrained the growth of B. tabaci as compared with control.
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Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns

 

...of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of interferon-stimulated genes (ISGs), responsible for playing an essential and decisive role in the virus pathology. Some studies have already reported the overexpression of these...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...isolates indicating very pathogenic nature of infection in the area. Risk factors determinants were found significantly (P<0.05) associated with mastitis occurrence except frequency of milking per day. Antibiogram of Staph. aureus indicated very strong resistance to oxacillin, ticarcillin, ampicilline, amoxicillin, azlocine, chloramphenicol, mupirocin, vancomycin, cefixinme, cefuroxime, and cefotaxime. In contrast to this sulphaphenazole, gentamicin,...

 Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy

...tance of major bacterial pathogens such as Escherichia Coli, Staphylococcus aureus Salmonella and Pasteurella spp. in Egyptian quail farms. Such information is important for drug choice and success of treatment as well as spotting the light on emerging antimicrobial resistance that represent major concern for public health. A total one hundred swabs and 500 organ samples were collected from apparently healthy and freshly dead quails respectively. Bacterial iso...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
...a (PED) was triggered by pathogenic coronavirus causing watery diarrhea and high mortality in piglets. This post-viral syndrome results in severe economic losses in the pig industry. However, limited information on the porcine epidemic diarrhea virus-infected pigs is available. Thus, the objective of this study was to identify transcriptomes with RNA sequencing (RNA-seq) in peripheral blood mononucleated cell (PBMC) of piglets, Large White x Landrace crossbred...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...align: justify;">The low pathogenic avian influenza viruses (LPAIV) subtype H9N2 are highly prevalent in the Middle East and are causing economic losses in the commercial poultry production sectors. Due to potential of H9N2 infections in human, LPAI H9N2 poses serious public health risk. Therefore, development of new potent and cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was ...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
...rofuse root galling. The pathogenic potential of M. incognita on passion fruit was determined; the minimum 10 second stage juvenile (J2)/200 cc soil can cause reduction of plant growth parameters and highest reproduction (R=87.27). 
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Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...the major features of AD pathogenesis. To provide the basis for further studies with this new animal model, we used high-throughput sequencing to analyze changes in the hippocampal transcriptome induced by injection of the three AD agents. These results open the door to new small-animal studies of AD that complement and extend studies in rodents and primates.
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Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...nst different food borne pathogens and quantification of the chlorogenic acid (CA) using High Performance Liquid Chromatography (HPLC). The chlorogenic acid was isolated using resin. The recovered amount of compound was calculated. Aliquots of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two a...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...genome sequence of human pathogenic bacteria Helicobacter pylori (H. pylori) strain 26695. Computational analysis of the genome showed 6 transcriptionally active pseudogenes that can produce stable mRNA secondary structure compared to their functional parents. Moreover it was observed that their putative protein products will be thermodynamically stable. The sequence-based predictions suggested that the pseudogenes-derived proteins may involve in...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molecular (nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies available to virologists ...

M. B. Meah

Biopesticides for crop growth and crop protection
...-off and seedling blight pathogens,Phomopsis vexans and Sclerotium rolfsii. In the field, garlic and allamanda spray reduced leaf blight severity by 84-86% and fruit rot by 91%. Severities of nematode and virus infection were also reduced significantly with allamanda spray. Active compounds in allamanda leaves have been separated and identified.Formulated trichoderma effectively controlled soil-borne diseases including root-knot nematode. Mahogany oil spray re...

Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram 

Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...olates belonged to plant pathogenic genera like,Alternaria, Cercospora, Colletotrichum, Curvularia, Glomerella, Mycosphaerella, Phoma and Stemphylium and the other 24 isolates of fungi belonged to Aspergillus, Acremonium, Chaetomella,Cunninghamella, Geotrichum, Gliocladium, Monodictys, Mucor, Myrothecium, Penicillium, Periconia and Pithomyces. One-hundred and thirteen isolates of chilli microflora and 49 isolates of Trichoderma, 19 isolates of Bacillus sp., 34...

Sitansu Pan, Surojit Khalko and Amrita Das

Effect of some fungicides on seed mycoflora, germination, viability and their persistence in treated seeds
...he highest appearance of pathogens viz.,Alternaria alternata was recorded in seeds of mungbean (Phaseolus aureus Roxb.), A. brassicae in mustard (Brassica campestris L.) and Drechslera oryzae in rice (Oryza sativa L.). Propineb 70WP and prochloraz 45EC considerably reduced the appearance of A. alternata and flusilazole 40EC reduced Fusarium sp.in mungbean seed than untreated control.Amarked reduction in population of A. brassicae by difenoconazole 25EC and flu...

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...mber of soil-borne plant pathogenic fungi, suppressive effects on some root nematodes without adversely affecting beneficial microbes like Rhizobium and capable of promoting growth of certain crops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production ...

S. K. Dutta, S. Roy Chowdhuri, D. Pandit and A. K. Bajpai

Rot diseases of muga host, Som (Persea bombycina) in Assam, India
...the infection of rotting pathogens on Som plants at Regional Muga Research Station (RMRS), Boko, Assam, India with during 2004-06. The disease incidence (DI %) caused by B. mediterranea was 12% while P. contigus showed 20% infection. Correlating the age of Som plants with susceptibility rot infection was found to be maximum in 10 years old plantation. Keywords: Rot disease, Som, Muga, Heart rot, Canker rot, Phellinus contigus, Biscogniauxia mediterranea, Assam...

Sitansu Pan and Someshwar Bhagat

Biological control of plant diseases
...oth bacterial and fungal pathogens. Smilarly, fungal-fungal, fungal-bacteria and actinomycete-fungal/actinomycete interactions exist in nature and opens a new area of research in biocontrol of plant diseases. The mechanisms of action of these biocontrol agents against target pathogens are antibiosis, mycoparasitism, competition for space and nutrient, production of certain toxins and secondary metabolites, solublization and ...

P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi

Botanicals for control of anthracnose of bell peppers
...otrichum capsici, fungal pathogen responsible for anthracnose disease in bell peppers (Capsicum frutescence L.). The results showed that all the selected plants have potential to inhibit the radial mycelial growth of C. capsici in-vitro. The plant extract from C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food technique, whereas 15% increased seed germination and 26% reduced disease incidence is recoded when compar...

Goutam Mondal

Plant growth promoting activity of some indigenous Tricho-derma isolates and their field performance against sheath blight of rice in old alluvial zone of North Bengal
...against soil-borne plant pathogens, namely, Fusarium oxysporum f. sp. ciceri (Padw.) Matuo. and Rhizoctonia solani Kühn. Efficacy in plant growth promoting activity of all the isolates was also tested on cauliflower (Brassica oleracea var botrytis L.), chilli (Capsicum frutescens L.) and tomato (Lycopersicon esculentum Mill.) with culture filtrate of biocontrol agents and found both positive and negative effect on seed germination, plant growth and vigour...

M.S. Islam, M.Ali, and I. Ahmad

Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
...f tea in Bangladesh. The pathogen, C. gloeosporioides was isolated from the dieback infected tea twigs on PDA medium. Mycelial blocks of the isolated pathogen were transferred to PDA plates amended with sedomil 72WP (mancozeb) @ 720, 1080, 1440 and 1800 ppm and recozeb 80WP (mancozeb + metalaxyl) @ 800, 1200, 1600 and 2000 ppm concentrations. Sedomil 72WP and recozeb 80WP caused colony growth inhibition, respectively by 75.0...

A. Sajeena and T. Marimuthu

Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...ue against sheath blight pathogen of rice was developed as an emulsifiable concentrate. The formulation of 10EC at 0.25% was standardized as the best emulsifiable concentration for use against the pathogen. The pre inoculation spray of the formulation was observed to produce 63.62% reduction in lesion height caused by the sheath blight pathogen. Two isoforms of poly phenol oxidase, PPO 1 a...

Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha

Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...ey, one isolate of entomopathogenic nematode from ginger rhizosphere was collected from Faizabad district of Uttar Pradesh (India). Morphological and morphometric studies identified the isolate as Stein-ernema carpocapsae. This was further confirmed by ITS-rDNA sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on...

M. S. Joshi , D.M. Sawant and A.P. Gaikwad

Isolate variations in Colletotrichum gloeosporioides infecting pomegranate
...inst six isolates of the pathogen. The interaction between isolates x varieties was also statistically significant. Highest DRI (15.22) was recorded in the interaction between Bhagwa x Cg 85 and it was on par with Ganesh x Cg 86, Aarakta x Cg 82, Aarakta x Cg 84, Ruby X Cg 86, Patna 5 X Cg 86 and Patna 5 X Cg 82.

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Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...8 plant oils against the pathogen Rhizoctonia solani was evaluated by disc diffusion method. Out of 44 plants tested, 36 plant extracts showed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera d...

Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta

Developing site-specific appropriate precision agriculture
... single major soil borne pathogen or pest across vast cultivated area but not for the peasant farming or the pest complex ridden unaided agriculture as in India. PA is not about an abstract of characterization. The specific values indicating variations in population at exact grids and helping the individual farmer in decision-making in pest management at varied pesticide doses depending on varied soil pest population intensity and spatial distribution. Often i...

Partha Pratim Ghosh and 2N. C. Mandal 

Some disease management practices for bacterial wilt of potato
...ection of bacterial wilt pathogen in seed tuber. Therefore, it is always desirable to obtain planting tuber from reliable source. So, integration between whole tuber planting or seed piece treatment units supervised management may offer better protection from the dreaded disease. Dynamic nature of disease management offers the scope for future modification.
 

 

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T. P. Ghosh, D. Mandal, S. Laha and M. K. Dasgupta 

Dynamics and severity model in managing fungal diseases
...he diseases (referred as pathogens) that were the highest and fastest during warm and wet (rainy) months were: Alternaria alternata on Polyanthes tuberosa; Alternaria alternata and Septoria chrysanthemella on Chrysanthemum indicum; Colletotrichum gloeosporioides and Alternaria sp. on Dracaena deremensis; Alternaria sp. and Cercospora hibisci-manihotis on Hibiscus rosa-sinensis; Colletotrichum gloeosporioides on Calathea ornata; Alternaria sp. on Ficus religios...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...l changes in response to pathogenic field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...tibodies production. The pathogenicity and etiology of the disease has yet to be elucidated. It is presently accepted that environmental factors trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a number of autoimmune diseases. We hypothesized that gluten containing diet would further exacerbate an already undergoing arbitrary immune reaction in SLE pa...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...prevalence of food borne pathogens in final packaged food products as well as for food preservation/ biocontrol, this study was designed to isolate lactoferrin from milk of camel. Cation exchange resin (CM-Sephadex C-25) was used for the isolation of lactoferrin using the fast protein liquid chromatography. The lactoferrin was further characterized through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied ...

Gadeeyya G and Ratna Kumar P.K.

...relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed. 
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Adnan Yousaf1,Jia Wu1, Qaiser Shakeel2, Yasir Iftikhar3, Muhammad Irfan Ullah4, Uzma Tahira5,Mustansar Mubeen1 and Wubei Dong1,*
...manage root rot and knot pathogen enhanced when it was integrated with bio-products. It was hypothesized that direct antagonism and defense by bio-product enhance the resistance in chili cultivars. So, T. harzianum is an efficient biological control for integrated management of chili plants under controlled condition cultivation. To explore the nature of resistance response of C-33 to M. incognita and S. rolfsii further research is needed....

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...>The incursion of highly pathogenic avian influenza virus (HPAIV) strain H5N1 in 2006 in Egypt caused severe economic losses for both commercial and backyard poultry production sectors. Owing to public health risk, effective control of the virus in imperative. DNA vaccination is a promising new approach for the prevention and treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by...
Rais Ahmed1,*, Khushi Muhammad1, Masood Rabbani1 and Muhammad Sarwar Khan2
...lecular diversity of the pathogens together with sero-conversion in animals and humans.
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Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...s an important potential pathogen which can control a wide range of bollworm larvae in both agricultural and horticultural crpos. Flubendiamide is a new chemistry insecticide which also controls bollworm effectively and relatively safer for natural enemies and beneficial arthropods. Present investigations were carried out to determine their impact on larval mortality, pupal and adult emergence of 2nd and 4th instar larvae of Spodoptera...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...ify;">Brucella is pathogenic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell cultur...

Muhammad Ibrahim1,2*, Syed Jawad Ahmad Shah1, Shaukat Hussain2, Musharaf Ahmad2 and Farhatullah3

 

Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
... agents against isolated pathogens at three different concentration viz., R, R/2 and R/4. Combination of bio agents (T+P) gave maximum results at R concentration against R. bataticola and M. incognita.
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Jawad Anwar* and Zafar Iqbal 

... against phyto and human pathogenic bacterial strains. T. harzianum was grown under different conditions and their extracts were evaluated, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Escheri...

Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3  

...es viruses are important pathogens that cause significant losses in the poultry industry. However, successful establishment of infection in the respiratory tract switch on the pathogen recognition by innate immune receptors and establish first line of defense against viral infections. When specific cellular receptors recognize a virus they trigger the translation of inflammatory cytokine and chemokines leading to establishme...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...asma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 2...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...determined role in tumor pathogenesis. A total of 52 tumor and normal samples were examined, among these, 40 canine and 12 feline samples were analyzed using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both species. While expression of c-Myc gene was found to be up regulated in different canine (62.5% in mammary ade...
Zahid Latif1,*, Kathrin Blasius2-4, Tufail Hussain Tahir7, Muhammad Nasim Khan1, Ghazanfar Ali5, Ansar Ahmed Abbasi6, Abdul Rauf1, Hao Hu8 and Angela M. Kaindl2,3,4
...B1 gene as an underlying pathogenic variant for non-syndromic autosomal recessive retinitis pigmentosa.
...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...opcorn disease and these pathogenesis-related proteins provide valuable information to further study the pathogenesis of popcorn disease and disease-resistant molecular breeding in mulberry.
...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*

 

...rmine the prevalence and pathogenic potential of Escherichia coli (E. coli) isolated from piglets having white score diarrhea as a result of outbreak occurred in Qinghai Tibetan Plateau in 2015. A total of 81 E.coli were isolated from 83 fecal samples. The organisms were inoculated on MacConkey agar and EMB agar and identified via biochemical tests. Polymerase chain reaction was used to detect representative virulence factors or genes, including ...

Imtiaz Ahmed, Muhammad Abbas Khan, Noorullah Khan, Naveed Ahmed, Abdul Waheed, Fazal Yazdan Saleem, Sajjad Khan and Sohail Aslam 

...Garlic rust is caused by pathogen (Puccinia allii) that severely hinders the productivity of Alliaceae family. Experiment was conducted to determine the optimum plant spacing in order to abate garlic rust and maximize garlic crop yield. The experiment was carried out in RCB design with three replications; garlic cultivar (china) and three levels of intra-row spacing (10, 15 and 20 cm) were included in the study. Data regarding disease occurrence, disease sever...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...revalence of potentially pathogenic intestinal protozoan parasitic infection in farmers, education concerned and shepherds of Swat, Khyber Pakhtunkhwa, Pakistan. A total of 1041 stool samples were examined from January 2006 to December 2008 using direct smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with singl...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...d binding protein in the pathogenesis of MetS.
...
Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
...r transferring a mass of pathogens i.e., West Nile Virus (WNV), avian pox virus (APV) etc. in rural and urban areas. To mitigate these problems study was conducted by using binary mixtures of entomopathogenic fungi, Beauveria bassiana (isolates Bb-01, Bb-10), Metarhizium anisopliae var. anisopliae (isolate Ma-11.1, Ma-2.4)and Isaria fumosorosea (isolates If-2.3, If-02) and chemical insectic...
Qingying Jia1, Hui Wang1, Tianhong Wang1, Na Dong1, Shuguang Ren1,2, Xiaolong Yang1, Jingze Liu1,* and Zhijun Yu1,3,*
...mit a great diversity of pathogens. The current study investigated the effects of temperature on reproductive fitness of the engorged H. longicornis, results indicated that the engorged H. longicornis could survive all treatments at 0°C and 4°C, and pre-ovipositon periods were prolonged after 0°C, 4°C and 40°C treatment, whereas it was shortened after 37°C stress (P<0.05). The oviposition periods were varied among diffe...

Ayesha Aihetasham*, Faiza Qayyum and Muhammad Xaaceph

Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4

 

...nfections of respiratory pathogens. However, impact of co-infections is not well known, especially in broilers. The current study was aimed to assess the probable synergism of E. coli (O78) and velogenic Newcastle disease virus in the broiler model. Three-week-old commercial broilers were inoculated with either vNDV, E. coli serotype O78 or both agents simultaneously or 3 days apart. The birds were clinically observed and swabbed daily. They were...

Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain 

...the only weed from which pathogen was consistently isolated. From Northern KP, a total of nine weeds including; Solanum nigrum L., Solanum americanum Mill., Solanum Sarrachoides Sedntner, Amaranthus blitoides S. Wats, Amaranthus albus L., Lactuca serriola L., Amaranthus retroflexus L., Malva parviflora L. and Sisymbrium irio L.were found to be possible sources of primary inoculum of the pathogen in tomato fields. 

...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...ple drug resistant (MDR) pathogens in milk of camels with characteristically zoonotic potential. Current study was planned to investigate epidemiology of MDR S. aureus in camel milk, and in-vitro therapeutic response to drugs. Two different ecological zones, Cholistan (n=185) and Suleiman Range (n=177) of Pakistan having major population of camel were screened for subclinical mastitis using California mastitis test using convenient sampling techn...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
...of potentially important pathogenic parasitic infections particularly in remote parts of the country.
...
Ali Murad Rahoo,1,2,* Tariq Mukhtar,1,3 Shoukat Ibrahim Abro,4 Barkat Ali Bughio5 and Rehana Kanwal Rahoo6
...The suitability of entomopathogenic nematodes as biological control agents of specific target insects is affected by their level of infectivity and reproductive capacity. Therefore, in the present study the productivity of five entomopathogenic nematodes (Steinernema feltiae, S. kraussei, S. carpocapsae, Heterorhabditis bacteriophora and H. indica) were compared in Galleria mellonella larvae. The product...

Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2* 

Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and  John E. Foster6
...v>...
Hina Safdar1,*, Nazir Javed1, Sajid Aleem Khan1 and Muhammad Arshad2
...ntrol potential of entomopathogenic nematodes (Heterorhabditis bacteriophora and Steinernema glaseri) against different larval instars (2nd, 3rd, 4th and 5th) of armyworm (Spodoptera litura F.) at different exposure times were evaluated. Entomopathogenic nematodes were applied at 1000 IJs/ml and larvae maintained at 25°C. Mortality was recorded upto four da...

 Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***

CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...wth by suppressing plant pathogens. The objective of this study was isolation and characterization of bacterial strains from rhizosphere, endosphere and rhizoplane of sunflower. Thirty six bacterial strains were selected out of 44 from plant root samples along with rhizospheric soil, collected from different salt affected areas of Central Punjab (Pakistan). Selected bacterial strains were characterized morphologically as well as biochemically at National Agric...

 Muhammad Mohsin Raza*, Muhammad Aslam Khan*, Irfan Ahmad**, Ali Ahsan Bajwa***, Hafiz Muhammad Usman Aslam*, Badar Ahsan Ullah****, and Kashif Riaz*

FOREST PATHOGENS AND DISEASES UNDER CHANGING CLIMATE -A REVIEW
...cks of climate change on pathogens and diseases. Biotic diseases, cankers, decays, declines, foliar diseases, root diseases and stem rust of pine have been reviewed with some illustrations of potential disease effects with predicted changing climate. The impact of changing climate on host, pathogen, and their interaction will have frequent and mostly unsympathetic outcomes to forest ecosystems. By employing the proactive and...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
...e major gastrointestinal pathogens in young ruminants and has been associated with diarrhea. The current study was conducted in three selected districts (Bannu, Lakki Marwat and Kohat) of southern Khyber Pakhtunkhwa (KPK) to determine the prevalence and risk factors responsible for the occurrence of Cryptosporidium in lambs and has been known to cause human infection. Three hundred and sixty fecal samples (n=360) were collected through convenient sampli...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...ganic materials. Several pathogenic microbial species utilize enzymes as virulence factors to cause diseases. The aim of the present study was to evaluate enzymatic activities of the bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidas...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Ali Murad Jakhar3, Rehana Kanwal Rahoo3
...mass production of entomopathogenic nematodes (EPN) are not yet available and the development and use of EPN mainly depends on the use of host insects such as greater wax moth (Galleria mellonella) for in vivo production. Since G. mellonella may not always be available, therefore, yellow mealworm (Tenebrio molitor) could be an alternative host. Therefore, in the present studies recovery of two EPN (Steinernema feltiae and ...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
...revealed the presence of pathogenic or opportunistic bacteria at the level of the average number of micro-organisms (11-50 CFU) or high (> 50 CFU), which negatively affected the health of the calves. However, in cold rearing there was recorded absence or presence of microorganisms in the nasal cavity in a small amount (<10 CFU).
...
Riyadh S. Aljumaah*, Mohammed A. Alshaikh, Abdulrahman Jarelnabi, Mutassim M. Abdelrahman and Mansour F. Hussein
...actors and to assess the pathogenic effects and economic consequences of neosporosis in camels.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...e hosts. Zoonotic highly pathogenic avian influenza virus (HPAIV) H5N1 of clade 2.2.1 has recently diversified into two distinct genetic subclades (2.2.1.1 and 2.2.1.2) in Egypt. This study was conducted as part of routine surveillance activities in Egypt; 5202 cloacal and oropharyngeal swabs were collected from live/dead birds, including commercial and backyard flocks, in five different locations in Egypt between the years of 2015 and 2016. All samples were s...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

...ement of important plant pathogens including root knot nematodes (RKN). In the present studies, 15 isolates of Trichoderma harzianum were collected from different the root knot nematodes-infested tomato field of Malakand and Swat, Pakistan. These isolates were tested for their nematocidal effects against Meloidogyne javanica. Anti-J2 antagonistic test divided the isolates into 5 antagonistic groups or AGs. AG1 was observed as the most antagonistic group, causi...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...that caries isolates are pathogenic S. mutans and soil isolates belong to the genus Streptomyces. The antibiotic resistance profile of S. mutans illustrated resistance to optochin. The antimicrobial screening of Streptomyces against S. mutans showed that these strain are the prolific producers of active agents to inhibit the growth of caries causing S. mutans. The natural extracts of Streptomyces strains signifi...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
...s. 

...
Zihong Chen1, Ling Xu2, Xiaona Yang2, Yaguan Zhang3* and Yuming Yang4
...arhizium is an entomopathogenic fungal genus relevant in biological protection. A new species of the genus, collected from Taibao mountain, Baoshan City, Yunnan Province, southwestern China, was described here as Metarhizium baoshanense. It was proposed and determined based on morphological characters combined with a multigene phylogenetics analysis involving 5.8S–ITS, nrSSU, nrLSU, EF–1α, RPB1 and RPB2. In multilocus phylogeny,
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...cine from the prevailing pathogenic strains of Type D Clostridiumperfringens strains and evaluate its immune responses in rabbits, goats and sheep. C. perfringens were isolated from enterotoxaemia suspected sheep and goats from the endemic areas of Khyber Pakhtunkhwa Province, Pakistan during 2016. The isolates were initially identified through colony characters, Gram staining and biochemical tests. The identified isolates were quantified ...

Wasim Javaid1, Farid Asif Shaheen1*, Farah Naz2 and Muhammad Usman Raja2 

...stify;">Aptness of entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae was evaluated to manage pulse beetle (PB), Callosobruchus chinensis by using different fungal concentrations of 1×106, 1×107 and 1×108 spores/ml in stored chickpea grains. Highest mortality (90%) of PB was observed in 72 hours through application of 1×108spores/ml of M. anisopliae compared to B. bassiana with 60% mortality. On 24 hours of expo...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5

G. Venkatesan* and Amit Kumar  

...rus, an important animal pathogen globally. Being zoonotic and endemic in most parts of the developing world, it is necessary to prevent and control the infection using latest molecular diagnostics and effective vaccine along with anti-viral therapeutics. This short commentary deals with current knowledge and opportunities on ORFV and future challenges as control perspectives in India. 

...
Muhammad Sarfraz*, Shahida Hasnain
...g resistance of clinical pathogens against several beta-lactam antibiotics. This study was conducted on 11,725 specimens collected from hospitalized and non-hospitalized patients from July 2013 to June 2016. API 20E system has been used to identify K. pneumoniae and E. coli; preliminary screening for AmpC beta-lactamase production was done by cefoxitin disc followed by inhibitor based confirmatory method. Antibiogram was performed following CLSI ...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...ognita and the entomopathogenic fungus, Lecanicillium muscarium. The initial inoculum was measured before okra was seeded in the field. At harvest, the numbers of root galls, egg masses, and eggs per root system, the root weight, the final population of the second stage juveniles, and the reproduction rate were detected at the highest levels in untreated soil, whereas low numbers were encountered in soils initially treated with the fungus,
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...measures to control this pathogen are needed, along with contemporary information about antibiotic resistances that are present in isolates from different environments. In the present study 72 samples from blood, 43 from sputum, and 19 were obtained from tracheal aspirates patients suffering from chronic and acute lung infections admitted to a local hospital in Lahore. Susceptibility of 134 isolates of P. aeruginosa was tested against selected antibioti...
Xiaqing Chen, Yü Huang and Zhen Huang*
...hiraiana is a fungal pathogen that attacks mulberry fruits and causes disease named as “popcorn disease”. Many Trichoderma spp. produce a series of antibiotic metabolites that can inhibit plant pathogens. Trichoderma hamatum isolated from infected mulberry fruits was evaluated for its efficiency to suppress plant pathogen S. shiraiana alone or in c...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
...um is a serious phytopathogen resulting in significant loss of crop yield. Trichderma hamatum has the capacity to inhibit S. sclerotiorum growth by production of toxic metabolites and mycoparasitism. In vitro bioassays were used to evaluate the efficacy of T. hamatum and difenoconazole alone or its combinations against S. sclerotiorum. The results showed that T. hamatum could infect and destroy the mycelia and scle...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...eus, the most common pathogen,using conventional microbiological and molecular methods. The agar disc diffusion method was used to evaluate the antibiotic susceptibility. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalospo...

Asinamai Athliamai Bitrus1*, Olabode Mayowa Peter2, Muhammad Adamu Abbas3 and Mohammed Dauda Goni

...being a highly versatile pathogen can quickly acquire resistance genes. The development of resistance in bacteria predates the era of antibiotic use. However, resistance developments in S. aureus have been reported since the early 1940-ties, when penicillin resistant S. aureus was first reported. Ever since, this pathogen has gain global notoriety as the most common cause of nosocomial, community and livestock associated inf...
Haroon Rashid1,*, Khushi Muhammad1, Masood Rabbani1, Muhammad Sarwar Khan1, Tasleem Ghori2, Mamoona Chaudhry3, Hamad Bin Rashid2, Muhammad Zubair Shabbir1, Muhammad Hamid Chaudhary4, Muhammad Asad Ali1, Mian Muhammad Khubaib Sattar5, Javed Muhammad1 and Bhushan Jayarao6
...(PA) in Chakwal District pathogen B. anthracis PA plasmid positive sample shows no association with distance from Animal Market, distance from road, distance from drainage, not subjected to human population, and for distance animal density because only one sample was found positive others districts (Gujranwala, Sargodha, Sahiwal, Faisalabad and D.G Khan) showed no association.
...
Qiang Li1,2, Guo Qiao2, Li Wang1, Jipeng Zhang1, Ruijun Li1, Ping Ni1, Yi Guo1 and Shigen Ye1,*
...plification, followed by pathogenicity determination. Results showed that the isolates belonged to 10 genera, including Vibrio (72%), Staphylococcus (9%), Pseudomonas (4%), Bacillus (4%), Vagococcus (3%), Shewanella (3%), Planococcus migula (4%), Exiguobacterium (1%), Enterobacter (1%) and Kocuria roseus (1%). Vibrio spp. and Vibrio harveyi were the predominant genus and specie...
Ali Murad Rahoo1, Tariq Mukhtar2,*, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
...div>The fitness of entomopathogenic nematodes (EPN) as biological control agents of specific target insects depends on their level of infectivity and reproductive capacity. Nematodes with higher levels of infectivity and reproduction within a specific target host may be more effective in controlling a particular insect under field conditions. The production of infective juveniles (IJ) from Galleria mellonella cadavers may differ among different species ...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...ultidrug resistant (MDR) pathogens, there is a demand of novel biological active compounds to replace currently used antibiotics in order to evade resistance. Currently, the pathogenic organisms of concern are methicillin and vancomycin resistant strains of Staphylococcus aureus, Acinetobacter spp., extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli, Klebsiella spp.<...

Zulqarnain and Zafar Iqbal* 

...hibition (%) against the pathogen. Penicillium sp. showed maximum growth inhibition (63.85 ± 3.26 %) and its extract showed 9.20 ± 0.14 mm zone of inhibition at 1000 mg·L-1 against the Fusarium wilt. Based on its higher in vitro antifungal activity, in vivo screen house experiments were conducted. Plants given the treatment as Penicillium + Fusarium showed slight yellowing of the leaves after 60th days of cultivation. Present results may h...

Mohsin Iqbal1, Farid Asif Shaheen1*, Farah Naz2, Muhammad Usman Raja2, Muhammad Fiaz3 and Muhammad Nadeem1 

...n current studies, entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana were used as the bio-control agents against this economic pest and proved good alternatives to chemicals. Less number of eggs (2 per grain), more number of holes (8.3 per grain), less number of F1 adults (20.3 per jar) and more number of days (7) to 100% mortality of C. chinensis adults were recorded when chickpea grains were treated with the highest concentration of B. ba...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi2*, Zillay Mariam1, Nadeem Sheikh1,2*
...h can house a variety of pathogens. As it drains it pollutes the groundwater table and if used for irrigation purpose represents a major health risk not only to humans but also to local flora and fauna. The purpose of current research work was to analyze the toxicity of leachate in Wistar rats. Wistar rats of about 250g were selected and divided into three groups namely Control, Group 1 and Group 2. Group 1 was given 4ml/kg leachate while Group 2 received 4ml/...
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
...ollusca (Glochdium). The pathogenic fungal genera isolated were: Aspergillus, Alternaria, Penicillium, Mucor. Blastomyces, Rhizopus and Fusarium. Ten bacterial genera were also isolated; Aeromonas, Pseudomonas, Streptococcus, Enterococcus, Acinetobacter, Bacillus, Moraxella, Lactobacillus, Staphylococcus,. Carnobacterium. There has not been a single case of topically acquired infection among students, laboratory...

Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3  

...ctivity against two phytopathogenic bacterial species (Clavibacter sp. and Xanthomonas sp.) and a human pathogenic bacterium (Escherichia coli) in 2016. Experiment was carried out by using complete randomized design with three replications in vitro. Phytochemical analysis reveals that the methanolic fraction of oil possesses metabolites like carbohydrates, amino acids, alkaloids, flavonoids and glycosides. To test the antiba...

Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1 

...its efficacy against the pathogen. The combination of M. piperita, A. indica and C. limon was superior in reducing the BLB of rice. Besides this, all treatments showed significant effect on the agronomic traits of rice plants. The reduction of disease in all the trials along with healthy crop stand in glass house and field indicated that these decoctions might play an important role in biological management strategies for the control of BLB of rice. The presen...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
...h the study of bacterial pathogens associated with irrigated wheat field water. Study was carried out to check the efficacy of heavy metals and antibiotics against five isolated bacterial pathogens. Different morphological and biochemical tests were performed to identify and characterize bacterial pathogens. Among isolated strains, two belong to genus Staphylococcus spp., other two were Ps...

Farah Deeba, Hafiz Abdullah Shakir, Muhammad Irfan, Javed Iqbal Qazi*

Chitinase production in organisms: a review
...yme have control of phytopathogens, physiological functions and degradation of chitinous waste. Interest in chitin wastes utilization is increasing day by day because of its natural resistance against degradation. The review focused on different sources of naturally chitinase production in organisms was discussed.

...

Saman Fatima* and Shazia Iram 

... economy. Due to various pathogens the quality of fruit gets diminished. One of such problem includes the occurrence of various skin disorders which leads to the rejection of fruit in international market. This study was designed in order to identify the pathogens that ruin the peal of Citrus reticulata Blanco (kinnow) in order to improve its quality. About twenty six sampled fruits of Citrus reticulata having damaged skin w...
Muhammad Asad Saleem1,*, Mirza Abdul Qayyum1, Mudssar Ali1, Muhammad Amin2, Muhammad Tayyab1,3 and Sumaira Maqsood4
...PW. Combination of entomopathogenic fungus (Bb) and Nitenpyram (Nit) were found more lethal to survival of RPW. A reduction in pupation and adult emergence was recorded in the combined treatments. Moreover, decrease in weight gain, frass production and cumulative gain in size was found when larvae were treated with integrated effect of Bb and Nit. Depending on the lethal of treatment, development duration of RPW was disturbed. Integration of Bb and Nit delays ...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...ication when both insect pathogens were used in combination (SpltNPV 1×108 POB/ml + Bt 0.1 μg/g) i.e. 85.25, 79.31 and 92.11% mortality (in case of 2nd instar larvae) and 70.42, 63.64 and 80.88% (in case 4th instar larvae) of Faisalabad, Chiniot and Sargodha’s population of S. litura, respectively. This study could be helpful to use NPV and Bt in intensive pest management under field c...
Huseyin Cetin1,*, Oner Kocak2, Emre Oz1, Samed Koc1, Yesim Polat1 and Kalender Arıkan2
...echanical vector of many pathogenic bacteria, fungi and parasites. The use of synthetic pyrethroid (SP) insecticides is recommended by the World Health Organization for the control of adult houseflies. Despite the fact that SPs have been used effectively, this insect has developed resistance in many regions/countries of the world. The use of synergistic substances to overcome insecticide resistance is widespread. Piperonyl butoxide (PBO) is used as a synergist...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
...ions for deploying entomopathogenic nematodes (EPN) in Pakistan can be harsh and the survival of infective juveniles (IJ) following inundative applications would be quite short. The application of EPN in cadavers may be appropriate because of the non-availability of industrially produced isolates. Therefore, in the present studies, Galleria mellonella and Tenebrio molitor were compared for invasion and production of IJ of Steinernema feltiae

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

...niques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculation method. The infection caused by S. scitamine...

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

...olates were assessed for pathogenicity based upon the mean death time (MDT), the intracerebral pathogenicity index (ICPI) and the intravenous pathogenicity index (IVPI) in chicken. The ICPI and MDT revealed that all tested isolates were of moderate virulence (Mesogenic) in chickens. Mature chickens showed no clinical signs or death as assessed by IVPI. Pigeon in Egypt reared in free rang s...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...mong major Gram negative pathogens is an emerging threat and a matter of great concern worldwide. Especially increasing resistance to 3rd and 4th generation cephalosporin and carbapenems is an issue of high importance. Present study was aimed to determine the frequency and antimicrobial susceptibility patterns of MBL producing carbapenem-resistant Gram-negative rods (GNRs) isolated from clinical specimens. A clinical descriptive study was...

Syed Atif Hasan Naqvi1*, Sidra Mushtaq1, Muhammad Tariq Malik2, Ummad-ud-Din Umar1, Ateeq ur Rehman1, Shoaib Fareed3, Muhammad Asif Zulfiqar

...nt. A diversity of plant pathogenic fungi has been identified and isolated from the various affected sheesham trees from Indian sub-continent, yet the actual cause of the diseases is still controversial. It is pertinent to mention here that no viruses or any bacteria has been reported from a singly tree in whole of the Indian sub-continent. Although the involvement of the insect’s species has been recorded and authenticated by various scientists reportin...

Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2 

...unflower is major fungal pathogen which can survive for months to years in soil and also over season in the seed. Therefore, population density and their aggressiveness to cause the disease is playing critical role for management of this pathogen. For such purpose colony forming units (cfu) per gram of soil and seed were determined and capability of previously characterized M. phaseolina isolates to cause the disease was tes...

Manzoor Hussain1, Muhammad Awais2, Farhatullah3, Saiqa Bibi1, Muhammad Rameez Khan3, Afifa Khalid1 and Sajid Ali3* 

...xt-align: justify;">Rust pathogens represent a major threat to cereal crops, though little is known about the status of oat crown rust in Pakistan. This study was designed to assess crown rust status and field based partial resistance of 16 exotic oat lines introduced from Europe along with local check, under natural field conditions at District Mansehra, Pakistan. Data on crown rust severity and host reaction was taken at three scoring dates to assess partial...

 Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1

...ples. Some opportunistic pathogens were also isolated from these media. Among the tested strains maximum amount of lactic acid (26.457 mg/mL) was produced by the lactobacilliafter 48 h growth in skim milk broth, while the least (12.131 mg/mL) was produced by pediococci. These LAB strains are being studied for their probiotic properties and good quality indigenous starter cultures from them are anticipated to be employed in food industry.

...

Amer Mumtaz2*, Muhammad Suhail Ibrahim1, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Masooma Munir2, Aqsa Qayyum2, Sahar Shibli2 and Muhammad Khalid Ibrahim3 

... for inactivation of the pathogenic organisms present in food by poration and DNA fragmentation. Ultrasounds have diversified application in meat industry, extraction of bio actives, dairy industry, Enzyme technology, fruit and vegetable juice industry, water, waste water treatment, starch modification and removal of food allergens from food. 

...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
... and control of such MDR pathogens.
...

Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1* 

...tudy, manure of specific pathogen free (SPF) chicks was contaminated with allantoic fluid of 8 log2 NDV and kept at room temperature then daily examination of poultry manure for the survival of NDV on the basis of virus isolation and haemagglutination assay (HA). Our results revealed that pH of the manure from time of its mixing with the NDV infected allantoic fluid till day 4 was 7, then pH values changed progressively and recorded 8.5 on day 12. After that, ...

Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1* 

...dy, manure from specific pathogen free (SPF) chicks was contaminated with allantoic fluid of NDV and kept at room temperature ranged from 20 to 25oc which is nearly the same as poultry farms on different coupon surfaces (metal, plastic and cooling pads). Daily examination of poultry manure for the persistence of NDV was conducted on the basis of virus isolation followed by haemagglutination assay (HA) which revealed that NDV survived in the manure up to 28 day...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

...c effect against another pathogenic microflora. Current studies were designed with the aim to search out such type of antagonistic bacteria from rice crop. For this purpose, soil was collected from rice growing areas of Tando Jam. The isolation from rhizospheric soil was done by dilution plate technique. Among isolated isolates, 6 representative colonies were purified, multiplied and characterized using light microscopy and other biochemical tests. The bacteri...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

...ay be due to transfer of pathogen from cow to buffaloes and from the environment in herds of mixed farming. The study results may be helpful in developing the strategic policies against the control of disease. 

...

Syed Atif Hasan Naqvi* 

...tiology, symptomatology, pathogen biology, disease development, disease cycle, epidemics and epidemiology, geographical distribution and strategies for the disease management viz., exploitation of host plant resistance, cultural, physical, chemical and biological control. Studies on pathogen variation have revealed that breeding with single major gene for resistance may be unsuccessful due to breakdown in resistance. Up to t...

Muhammad Ijaz, Sayed Rashad Ali Shah*, Muhammad Izhar-ul-Haq and Amir Afzal 

...d leaf assay against LLS pathogen. Most of genotypes during three years of study at early assessments under field conditions showed resistant response whereas, at late assessments most of genotypes were susceptible. In pathogen-host relationship several components of partial resistance have been proposed. Under detached leaf assay Virginia type groundnut showed minimum spots per leaf, sporulation, and product of spots per le...

Mehboob Arslan* and Xin Yang 

...e obligate intracellular pathogens and rely on host machinery to successfully cause infections. In order to succumb hosts, viruses have evolved strategies to manipulate specific cellular metabolic pathways that aid the production of viral particles. Viruses need to breach a complex network of metabolic intrinsic factors and microenvironment established within the host cells. The pressure exerted by invading pathogen disrupts...
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...ny traits with the human pathogenic isolates based on virulence gene contents that may pose a potential threat to public health.

...
Mohsan Iqbal1, Farid Asif Shaheen1, Rashid Mahmood2,*, Muhammad Khalid Rafique2, Imran Bodlah1, Farah Naz3 and Muhammad Usman Raja3
...rgistic effects of entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae and entomopathogenic bacteria (EPB), Photorhabdus temperata and Xenorhabdus nematophila were studied as bio-control agents to manage this pest. In addition, percent conidial germination and % mortality of C. chinensis were also evaluated when B. bassiana was used with DEBBM. The minimum num...

Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan 

...solation of the H9N2 low pathogenic avian influenza virus in 2011, the virus has distributed rapidly and widely in different poultry sectors in Egypt causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, 1026 confirmed positive H9N2 cases by RT-PCR out of 23182 different examined samples of different species and secto...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48 h) was more active, being able to kill 100% of th...
Zhimin Yuan1, Yan Yu2, Yanmei Wang1, Yanzhen Bu1,* and Hongxing Niu1,*
...al reservoir of zoonotic pathogens. Some strains of bacteria in the gastrointestinal tract of bats are pathogenic to humans. Hypsugo alaschanicus feeds on insects and has a wide geographic distribution in China, and people are in frequent contact with these bats. However, assessing the gut microbiota, especially the potential pathogens, is needed for public health. Thus, this study ...

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...solation of the H9N2 low pathogenic avian influenza virus (LPAI) in 2011, the virus has been distributed rapidly and widely in different Egyptian poultry sectors causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, a total of 23182 cloacal and tracheal samples were collected from suspected cases between 2015 and 2...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
...ss-reactivity with other pathogens. Sensitivity testing showed the minimum detection limits of real-time (RT)-PCR were 7.44×101 copies·μL-1 (CPV) and 4.20×101 copies·μL-1 (CDV). DARPM detection of CPV with DNA showed a minimum detectable of 1.53×101copies μL-1, while the minimum detectable amount from the virus culture supernatant was 6.70×101 copie...

Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan 

...rmore, the intracerebral pathogenicity index (ICPI) for selected 5 virulent viruses reveals velogenic features with high pathogenicity index (1.60 to 1.74). 

...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

... of the strains were non-pathogenic and potentially industrial strains for the production of thermolabile protease and alkaliphilic lipase 

...

Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian, Jonathan Hartanto Tan and Zheng Shusen*
 

... (HBV) as a deadly viral pathogen and crucial challenge for mankind is directly involved in the progress of hepatocellular carcinoma (HCC) subsequently affecting 250 million human population. Due to the complex viral genome of HBV, complete recovery from hepatitis is not achieved. Epidemiologically, it has been classified into infectious and serum hepatitis. The pattern of disease progression manifests liver inflammation, chronic hepatitis and hepatocellular c...

Wesam Mady*, Nahed Yehia, Mohammed Hassan Elhussieny, Azhar Gaber Shalaby, Moataz Mohamed El Sayed, Neveen Rabie Bakry, Asmaa Shaaban, Abdel Satar Arafa, Afaf Abdel Baset Mahmoud and Wafaa Mohammed Hassan 

...ince incursion of highly pathogenic avian influenza virus (HPAIV) H5N8 in November 2016 in Egypt, it caused severe economic losses for both the commercial and backyard poultry production sectors. The aim of this work is to study the Situation and molecular characterization of HPAIV (H5N8) in backyard poultry production sector in Egypt. In this study, a total of 7505 samples of tracheal swabs representing 1180 backyard poultry flocks were collected based on the...
Shujjah Haider1,*, Ayesha Maqbool1, Tariq Pervez1, Saima Parveen1, Arfan Ahmad2, Zahid Iqbal3, Javed Iqbal3, Shahid Mehmood3, Amanullah Khan4 and Sajid Umar5
...stry every year. Various pathogens can initiate respiratory diseases in poultry, including mycoplasmosis caused by Mycoplasma gallisepticum (MG). The distribution pattern of MG in layer flocks is not known in Chakwal. Keeping this in mind, current study was designed to know about distribution of MG. The present study was conducted on 25 commercial layer flocks from different regions of district Chakwal, Pakistan. A total of 358 blood samples were collec...
Zil-e-Huma1, 2, Abdul Malik Tareen3, Kaleem Ullah3, Tauseef M Asmat4,Abdul Samad4Asim Iqbal2, Mohammad Zahid Mustafa3*,Irshad Ahmad5 and Sadeeq ur Rahman6
...li pathotypes (enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). Results show that the virulent EAEC strain of E. coli was found to be the most prevalent (35%), while EPEC was identified in 15%, followed by EHEC (8%). Notably, none of the samples was found positive for EIEC. Moreover, ETEC was ident...

Muhammad Usman Ghazanfar, Muhammad Imran Hamid, Mubashar Raza, Waqas Raza*, Misbah Iqbal Qamar 

...against soil borne plant pathogens. The present study was conducted with the aim to determine the efficacy of Trichoderma species using dual culture and pot assays against Phytophthora infestans (late blight) and Fusarium oxysporum (wilt) of tomato on different compost including carbon rich compost, nitrogen rich compost and nutrient enriched compost. The species of Trichoderma includes T. harzianum and T. asperellum isolated from rhizosphere of tomato from di...
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...ion to the occurrence of pathogenic diseases. Fruit samples (injured and healthy) were taken from the three different local markets of Karachi. Some of the fungal species viz. Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Rhizopus stolonifer and Alternaria alternata have been identified in citrus samples. However, major incidence was recorded for Aspergillus niger (41.6%) followed by Fusarium oxysporum (27.7%). Infected...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...rains of AAvV-1s and low-pathogenic H9N2 avian influenza viruses are presently endemic in Pakistan and repeated outbreaks are continuously being reported with high mortality in poultry and non-poultry avian species. In this study, five AAvV-1s have been pathotyped and genetically characterized from vaccinated birds collected between 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99....
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...sence and count of fecal pathogenic bacteria e.g. E. coli and Salmonella spp. Forty lactating Holstein cows, weighing 550±50 kg, with an average of 20 kg daily milk yield, were randomly divided into four groups (ten in each group). Animals were fed rations containing DOP at 0, 25, 50 and 75% substitution of yellow corn grains (R1, R2, R3 and R4, respectively). Digestion trail was conducted in the last week of the experiment using Ac...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Naimat Ullah Khan3, Muhammad Asfandyar Khan2, Kashif Prince1,*, Mahboob Ali1, Ghazunfar Rashid1 and Azmat Ulah Khan2
Abdul Hanan1, Talha Nazir1, Abdul Basit1, Shahbaz Ahmad2 and Dewen Qiu1,*
...The utilization of entomopathogenic fungi as potential microbial control against different insect pests is important due to their eco-friendly nature. The virulence potential of 3 different isolates of Lecanicillium lecanii formerly recognized as Verticillium lecanii (V-2, V-3 and V-5) with different bioassay materials (conidia, filtrate and the binary combination of conidia + filtrate) were evaluated against green peach aphid (Myzus persicae<...
Nazish Mazhar Ali1, Asma Ashraf 2*, Iram Liaqat2, Bushra Mazhar2, Shaukat Ali2 and Saiqa Andleeb3
...isolate and characterize pathogenic bacteria from eye infections. The samples were taken from corneal eye infections. The collection of samples was done from “Layton Rehmat Eye Hospital, Township Lahore”. Isolated bacterial pathogens were identified on the basis of morphological and various biochemical tests. Morphologcal tests included different staining procedures includng Gram’s staining and Endospore st...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
... analysis to detect this pathogen in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the les...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...eshawar to collect entomopathogenic nematodes, Insect bait technique was used for Entomopathogenic nematodes collection. For this experiment, Galleria mellonella larvae were reared in the laboratory. When  samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post applica...

Abdul Razzaq1*, Muhammad Islam2, Zahra Islam3, Zahida Fatima1, Munib Hussain4 and Farmanullah5,6 

...nal nematodes are common pathogens in grazing sheep/goats throughout the world which impairs productivity and leads to high economic losses. In most part of the world, drug resistance against anthelmintic is now very common. In this context, various alternative control programs including herbal use against these worms is also an important option. To explore the problem, three available synthetic anthelmintic (Oxfendazole alone, oxfendazole-Levamisole combinati...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...ections particularly low pathogenic avian influenza virus (LPAIV) H9N2, which can bring exceptional abatement in trade of poultry products in endemic countries such as Pakistan. The current study was conducted to evaluate the infectious and antigenic potential of three different AIV (A/H9N2) variants isolated from commercial, backyard poultry and fancy birds (partridge). Commercial broiler birds were infected experimentally at the dose of 106 EID
Shaukat Ali1,*, Sadia Batool2, Fatima Javed Butt2, Sundas Nasreen1, Hafiz Muhammad Tahir1
...e, immunomodulatory, antipathogenic and anti-cancerous properties. Review based on the futuristic and modern aspects of cancer treatment, we believe that probiotics based cancer treatment would be supportive to overcome the chemotherapeutic and radiological drawback sand provides an appropriate cancer treatment strategy. Anticipating various challenges, we expect that probiotics may become a potential cancer bio-theranostic in the field of medical sciences sho...

Khaled Kaboudi* 

...ext-align: justify;">Low pathogenic avian influenza H9N2 subtype virus is considered among important respiratory pathogens in poultry worldwide. It was isolated from many species of domestic and wild birds. Infection in commercial poultry flocks causes important economic losses, related to high mortality, decrease in animal performance and aggravation of infection by other pathogens. Altho...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
...istinction life. Precise pathogenesis job rheumatoid factor not known, through formation immune complexes engross establishment harmonize. To determine the correlation between Anti-Oxidants and Biochemical status in Rheumatoid Arthritis patients. 60 clinically diagnosed Rheumatoid Arthritis (RA) patients and 50 healthy persons were included in this study. 5 ml Blood was drawn and serum was separated. Anti-Oxidant Biomarkers, Serum micronutrients, serum Electro...
Khursheed Ahmed, Shoaib Freed*, Rana Fartab Shoukat and Kanwar Waqas Ahmad
...of each isolate of entomopathogenic fungi were used in combination with insecticides against 5th instar nymphs of O. hyalinipennis. The combined treatment of isolates of entomopathogenic fungi and synthetic insecticides caused higher mortality of O. hyalinipennis. Moreover, the combined treatments decreased the adult emergence, male and female longevity, while increased the nymphal duration. The resu...
Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*
...effector pathways in the pathogenesis of Non-Hodgkin Lymphoma (NHL). Binding of growth factors/ mitogen/cytokine or interleukin to EGFR leads to Ras induced RAF-MAPK cascade activation. PTEN protein is involved in the negative regulation of PI3K pathway. Mutations in upstream kinases, growth factor receptors or intrinsic members of cascades can lead to induce or promote cancers. Number of somatic mutations in several genes, majority of which are involved in ch...
Erum Yawar Iqbal1*, Ashfaque Ahmed Nahiyoon1, Shahnaz Dawar2 and Shahina Fayyaz1
...ntified indigenous entomopathogenic bacteria (EPB) Xenorhabdus have been used to access their efficiency against cotton Aphid Aphis gossypii (Glov.). An efficient formulation of different parts of bacterial culture such as, cell-free supernatant, crude cell extract, bacterial culture and methanol extract in in vitro and green house condition have been exploited. The results have shown that different formulants of EPB produced significant e...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...obial resistance of this pathogen, a total of 360 chicken cloacal swabs and 15 water samples were gathered from different localities in Giza and Cairo Governorates. An additional 50 stool specimens were collected from individuals in contact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (PCR) as C. jejuni (63.6 %) ...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...nst multi drug resistant pathogens and to characterize the bioactive metabolites produced by them. A total of seventy strains of actinomycetes were isolated from lake samples, out of them only forty bioactive strains were selected for further characterization on the basis of their preliminary antimicrobial activities. The isolated actinobacterial strains showed an optimum growth at alkaline pH (9.0-9.5). The taxonomic analysis revealed that these isolates were...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
...font-size: small;">Entomopathogenic nematodes (EPNs) are considered as very effective biological agents against several soil dwelling pests. The following research demonstrates the reproductive potential of EPN specie Steinernema kraussei against last instar larvae of four lepidopteran insects, wax moth (Galleria mellonella), pink bollworm(Pectinophora gossypiella), eggplant fruit borer (Leucinodes orbonalis) and armyworm (Spodop...
Ali Asghar1*, Tariq Mukhtar1, Muhammad Usman Raja1 and Asim Gulzar2
... as a result of both the pathogens applied singly and in combinations. The reductions in these parameters were significantly higher when both the pathogens were applied simultaneously as compared to their sole applications. Similarly, at higher inoculum densities, the reductions were the maximum in all these parameters. Significant variations were observed in number of galls and egg masses per root system in response to both...

Muhammad Zeeshan Nazar*, Ayyan Umer, Zahid Mahmood Sarwar, Faiz Ullah Faiz and Sikandar Hussain 

...s susceptible to various pathogenic diseases. Among the all bacterial diseases of B. mori, flacherie is injurious disease that is initiated by entomo-pathogenic bacteria, Bacillus thuringiensis var. sotto (Bts). The impact of this infectious disease is weight loss, reduction in cocoon quality as well as quantity and source of infestation for healthy larva. In current study, the 3rd instar larva of B. mori were fed on the lea...

Shilong Chen1,2, Shifeng Xiao1,2, Shao Wang1,2, Fengqiang Lin1,2, Xiaoxia Cheng1,2, Xiaoli Zhu1,2, Dandan Jiang1,2, Shaoying Chen1,2* and Fusong Yu3* 

...genomic organization and pathogenesis. However, the pathogenesis mechanisms of cross-species transmission, retardation and beak atrophy are still needed to be elucidated. 

...

Ahmad-Ur-Rahman Saljoqi1, Muhammad Zubair Khan1, Ayesha Bibi2, Muhammad Shehzad Khan1*, Bashir Ahmad

...ical characterization of pathogen of tomato stem rot and to evaluate the effect of different doses of boron, plant extracts and a bactericide in different combinations on tomato plants. This research study was performed at Agricultural Research Institute, Tarnab, Peshawar-Pakistan. Different biochemical tests were conducted to confirm stem rot pathogen. Boron was applied at the rates of 1.97, 2.96 and 3.95 g seedbed-1. Plant...
Şükrü Önalan1*, Muhammed Arabacı1and Haşmet Çağırgan2
...e is one of the main pathogenic agents in rainbow trout farms in Turkey. Twenty-two L. garvieae isolates obtained from different regions in Turkey were evaluated phenotypically in the study. In all isolates, cream colored, bright, round and S-type colonies with smooth margins were seen in TSA medium. They were alive during native examination without movement. It was observed that morphologically all isolates were Gram (+), α-hemolytic (BA), ox...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
...oteins in ticks or other pathogens but not in H. longicornis. Few reports have described dual specificity phosphatase 3 (DUSP-3) in parasites as an antigen. We successfully screened four potential antigens and found that DUSP-3 is a potential novel antigen for ticks.
...

Asmaa Abdallah Darwish 

...different ovine diseases pathogenesis and complications and their supplement for healthy and diseased animals is strongly recommended.  

...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...in 2016. We isolated the pathogenic bacteria (HNM-1) from the infected A. sinensis and identified its identity by conventional physiological, biochemical, and molecular techniques. Virulence and pathogenesis of the disease were determined by intraperitoneal injection of the etiological agent to the healthy A. sinensis. Physiological and 16s rRNA molecular analysis identified Streptococcus iniae as t...
Muhammad Anwarullah1*, Aneela Zameer Durrani1, Muhammad Ijaz1Aftab Ahmad Anjum2, Ghulam Mustafa3, Muhammad Husnain1 and Muhammad Usman1
...otal of 40 SPF (specific pathogen free) rabbits were selected at the age of two months. Rabbits were given experimental challenge with oral inoculum of pure culture of MAP. These rabbits were divided into four groups; three test and one control group. Histopathological lesions were observed before and after the therapeutic trials and lesions scoring was given according to the standard. On the basis of the changes in histopathological lesions, the effect of the...

Muhammad Usman Ghazanfar1, Waqas Raza1*, Waqas Wakil2,3, Imtiaz Hussain4 and Misbah Iqbal Qamar

...resistance against plant pathogens and insect herbivores. The plan of the study was to study induction of defense responses and protective effects against Phytophthora infestans (Mont.) de Bary and sucking insect pests of potato (Solanum tuberosum L.) with application of salicylic acid (SA) and β-aminobutyric acid (BABA). Concentration of SA and BABA (2, 4 and 5 mM) were applied to foliage. Application of both resistance inducers suppressed disease develo...
Shaheen Rahman1, Kafeel Ahmad1*, Niaz Ali2, Muhammad Idrees3 and Waqar Ali4
...mefaciens is a plant pathogen that causes devastating Crown Gall disease. Current studies were aimed at the isolation of A. tumefaciens from local soil. Five soil samples were collected from research farm of Agricultural University Peshawar, Pakistan. Soil samples were processed for the isolation of A. tumefaciens. Various biochemical and morphological tests were conducted to confirm the identity of A. tumefaciens. The

Gulnaz Parveen1*, Salma Gul2, Muhammad Ather Rafi3, Ashfaq Ali Khattak4 and Hikmatullah Jan

...ity for different fungal pathogens and have compounds are more effective to promote the growth of tomato plant. Tomato (Lycopersicum esculentum) plant is highly susceptible to root rotting fungi causing huge losses each year in Pakistan. Acacia nilotica used as a biofertilizer and Significant (p< 0.05) enhanced the growth of tomato plants. Infection % of all test fungi included Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina and Rhizoctonia sol...

Iram Fatima1*, Imran Pasha1, Ambreen Saddozai2, Shahid Nadeem3, Amer Mumtaz4 and Saqib Jabbar4 

... plate count checked and pathogenic count was calculated in CFU/g and CFU/ml, mean CFU for E. coli and TPC was (3.39×104 to 3.15×105). Total 47.9% samples of spices were found adulterated for eight different adulterants tested. Sugar samples were free of any adulteration while in beverages two other adulterants were found in 22% of samples. Analysis of variance (ANOVA) under two way factorial indicated signif...
Saira Saleemi1*, Zafar Iqbal1, Abdul Nasir Khalid2
...nfirmed by isolating the pathogenic fungus from affected skin, gills, fins, heart, liver, kidney and intestine of fish and culturing it on four different culture media; Malt extract agar (MEA), Corn meal agar (CMA), Sabouraud dextrose agar (SDA) and Potato dextrose agar (PDA). The inoculated culture plates were incubated for 5-8 days at 28-32 oC. The fungal growth in the form of colonies of different shapes and colour appeared in agar plates. Four <...

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

... to be relevant to HIV-1 pathogenesis and disease. HIV-1 genome has nine genes; gag, pol, and env genes are common in all replication-competent retroviruses, and the pol gene is very unique encoding the enzymes for replication. Our objective is to describe the relatedness of these HIV-1 pol genes with each other and its antiviral drug susceptibility which will provide more information on its sensitivity and resistivity to each antiviral drug. Seventeen partial...

Hafiz Abdul Ghafoor1*, Muhammad Afzal1, Muhammad Luqman2, Muhammad Arshad Javed2, Syed Wasim Hasan3 and Muhammad Zeeshan Majeed

...mbination with the entomopathogenic fungus Beauveria bassiana (@ 1x108 conidia mL-1). First experiment was conducted in March 2016 and 2nd was performed from November 2016 to March 2017 in a citrus (Citrus reticulata cv. kinnow mandarin) orchard. In first year field experiment, there was a significant impact of all treatments on the reduction of mealybug infestation (F9, 39 = 39.10, P < 0.001; HSD at α = 0.05) as compared to control plants. Results of...

Kajol, A. H. Bhat, Aasha and A. K. Chaubey

Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India
...urther, we evaluated its pathogenicity against Galleria mellonella and Helicoverpa armigera. The LD50 value of strain DH3 against G. mellonella larvae at 48 hours was 8 IJs/larva for 50% larval mortality whereas in Helicoverpa armigera it needed 13 IJs/larva, to kill 50% of the larvae.

...

M. Rufai1† , A. A.Wahab2 , Q. O. Adeshina1 , J. C. Umehokoh1 and O. O.Ologunro1

Occurrence of entomopathogenic nematodes in Osun State, Southwestern Nigeria
... the occurrence of entomopathogenic nematodes (EPNs) was determined in three senatorial districts of Osun State in relation to soil texture, vegetation, moisture and pH. A total of 110 soil samples were randomly collected from various cultivated fields in different locations. The soil samples were baited twice using last instar larvae of the Galleria mellonella (greater wax moth) for presence of entomopathogenic nematodes. F...

Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1

Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
...icola was found as a new pathogen causing stem rot of potato in the potato growing area of district Kasur. Symptoms of the N. rubicola infected potato plants were necrotic stem lesions near the collar region. Causal organism was isolated from the infected tissues, purified and identified on the basis of morphological characters, nucleotide sequences of internal transcribed spacer region (ITS) and partial beta tubulin gene. Phylogenetic analysis was also conduc...
Muhammad Khan1, Tehmina Ameer Khan1, Aziz Ud Din2, Muhammad Fiaz Khan1, Irfan Ullah3,*, Kalim Ullah4, Sadia Tabassum1,*
...6S-rRNA gene are the pathogenic mutations associated with HCM in Pakistani population.
...
Muhammad Rizwan1*, Bilal Atta1, Ana Maria Marino de Remes Lenicov2, Roxana Mariani2, Arshed Makhdoom Sabir1, Muhammad Tahir3, Misbah Rizwan4, Muhammad Sabar1, Ch. Muhammad Rafique1, Muhammad Afzal5
...n vectors of severe rice pathogenic diseases in the Oriental and Paleartic regions. Laodelphax striatellus was recorded on rice for the first time in Pakistan. Among alternate hosts, Trifolium alexandrium, Leptochloa chinensis, Helianrhus allus, Medicago polymorpha and Sorghum bicolor were recorded for L. striatellus, while Leptochloa chinensis, Helianrhus allus, Medicago polymorpha, Sorg...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...e Aloe Vera against most pathogens found in the wounds of donkey. For this purpose, two different concentrations 30 µl and 60 µl crude as well as ethanol extract of Aloe Vera were used. During this experiment, total 50 wound samples were collected from donkey wounds. All 50 samples were found contaminated with different bacterial organism, among all those 45, 35, 33, and 25 were recorded positive for S. aureus, E. coli, Shigella and Salmo...
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...despread and destructive pathogen of tomato, difficult to control due to its wide host range. In present investigation effect of bio (Cure, Azadirachtin) and synthetic (Cartap, Virtako) chemicals was evaluated on the induction of systemic activity by using modified split root technique. Efficacy of different application methods; soil drench and root dip was also tested. Results revealed that all the chemicals have more or less systemic effect against M. inc...

Amir Afzal, Muhammad Ijaz and Sayed Rashad Ali Shah*

Determination of Suitable Growth Stage for Application of Fungicide against Stripe Rust in Wheat
...of fluctuating nature of pathogen. Therefore, an alternative management strategy to control diseases is application of suitable fungicide at appropriate growth stage. For this, an experiment was conducted to investigate proper growth stage for application of fungicide to control stripe rust in wheat. Sowings at various dates was done to attain various growth stages at once. Two genotypes 16C038 and cv. Ihsan 2016 were selected for study. Difenoconazole 25% was...

Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5

Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
...asma citri, a fastidious pathogen, is considered as the main cause of Citrus little leaf disease (Citrus stubborn disease) which is not only responsible for the low citrus production in terms of quality and quantity but also moving the citrus industry to down fall in citrus growing areas throughout the world. Current research was organized to study the distribution of disease using the serological based detection methods by conducting a comprehensive survey in...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...lex is caused by various pathogens, including diverse type of Fusarium species. Molecular approach is a useful technique for the identification of wilt complex pathogens. The study conducted to identify the polygenetic association of the pathogens of chickpea wilt complex. Universal Rice Primers based diversity analysis indicates high polymorphism within the collected wilt isolates. Primer...
Chao Zhao1,2, Sigang Fana2 and Lihua Qiua2,3,*
... are susceptible to many pathogens and water quality and diseases affect commercial shrimp production, the relation between these factors are less studied. Therefore, we aimed to investigate the effect of bacterial dynamics and water quality factors on Penaeus monodon larvae. We recorded the survival rates, water quality (total ammonia, nitrite, and nitrate concentration), heterotrophic bacteria, and vibrios during different P. monodon stage...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...for multidrug resistance pathogenic plants and human bacterias. 

...

Muhammad Imran1,2*, Shahbaz Talib Sahi1, Muhammad Atiq1 and Amer Rasul3

Brown Leaf Spot: An Exacerbated Embryonic Disease of Rice: A Review
... nutrient deficient. The pathogen damage is compensating by a specific nutrient that reduces the disease through tolerance. Good management practices are those which include all possible combinations of cultural, biological, chemical, induce resistance, nutrient management, natural byproducts, and resistant cultivars. The best control of this disease in current climate scenario is the use of the integrated different management approaches to cope the emerging t...

Zulnorain Sajid1*, Muhammad Ramzan2 and Noreen Akhtar1

Foraging Behavior and Pollination Ecology of Bumble Bee and Honey Bee in Pakistan; A Review
...ical, exotic species and pathogens) involved in the declining of pollinators, a big loss for the economy of Pakistan. The aim of this review paper was to check the importance of honey bee and bumblebee as chief pollinator of agriculture crops. This review indicates how declining factors are alarming for the bumblebee and honeybee.

...
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...tant food borne zoonotic pathogens. A total of 1260 poultry meat samples were collected from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicro...

Muhammad Tariq-Khan1*, Syed Zanib Ali Gardazi1, Abu Daud Ahmad Khan1, Muhammad Ilyas2 and Ishaq Ahmad3,4

Virulence and Distribution Trends of Root-Knot Nematode (RKN) Fauna on Summer Vegetables in District Bagh, Azad Jammu and Kashmir (Pakistan)
... Understanding regarding pathogen survival under adverse conditions will help the scientists to develop new approaches for sustainable yield.

...

Shahid Ahmed1,2*, Huiqing Su1, Xuqian Sun1, Peng Feng1, Yongpan Chen1, Liang Yu1, Qian Liu1 and Heng Jian1

Performance of Cereal Varieties against Cereal Cyst Nematode (Heterodera avenae)
... justify;">The soilborne pathogen cereal cyst nematode (CCN) Heterodera avenae is one of the biotic stress factors that directly affects the crop physiology from seed germination to crop maturity and limits the cereal production in different agro-ecologies of the world. In China, this pathogen is widespread in more than 20 provinces and infects the major cereal varieties and germplasm being adopted for cereal crop production...

Muhammad Javaid1*, Unsar Naeem-Ullah1, Waheed S. Khan2, Shafqat Saeed1, Mirza Abdul Qayyum1 and Muhammad Arslan Khan1

Role of Nanotechnology in Crop Protection and Production: A Review
...anofungicides to control pathogens, plant diseases and nanoherbicides for the management of weeds. It also facilitate in precision farming, targeted use of inputs i.e. nanofertilizers and to overcome the environmental stress seed treatments with nanoparticles, to improve the crop yield use of smart gene delivery system. This technology can also be useful for sustainable water use and irrigation water filtration. It also plays an important role in pollution red...
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
.... This is due to complex pathogenesis comprising a network of signaling pathways. By controlling one pathway, the pathogenesis continues through complementary pathways. Janus kinase and bruton’s tyrosine kinase regulates a number of T-cell and B-cell mediated signaling pathways ranging from cytokine expression and antibody production to the bone resorption and cartilage destruction. We propose a dual trap to control th...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...> are important zoonotic pathogens. Brucellosis is an occupational hazard for veterinarians and animal handlers. The current work aimed to determine the prevalence of brucellosis in buffaloes and goats of Islamabad Capital Territory, Pakistan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161) were screened by (Milk Ring Test) MRT and milk i-ELISA for anti-Brucella antibodies. Higher prevalence was found in buffaloes (5.6% and 16.1%) t...
Adeen Kiran1, Umar Farooq Gohar1*, Ayesha Farooq1, Malik Muhammad Asif2 and Hamid Mukhtar1
Redressal of Antibiotic Resistance using Plant Extracts
...aused by these resistant pathogens. In this battle, there is a continuous need to develop new drugs or compounds that can cure these infections or to reverse the antibiotic resistance in these pathogens. In recent years different experimental approaches highlighted the importance of phytochemicals present in plants and its ability to kill these pathogens and microbial inability to develop ...

Muhammad Iqbal1, Muhammad Ehetisham ul Haq1*, Muhammad Kamran1, Muhammad Idrees1, Shahid Nazir2, Ihsan Ullah3, Sumera Naz1, Shaukat Ali1 and Muhammad Zafar Iqbal2

Morpho-Molecular Characterization of Xanthomonas Axonopodis Pv. Citri Associated with Kinnow (Mandarin) and its Management
... antibiotics against the pathogen was evaluated in the lab and in field conditions. A systematic survey of randomly ten localities/sampling sites was conducted for a reliable estimation of citrus canker disease in District Sargodha of Punjab Province. Nine sampling sites showed 90 % the presence of Xanthomonas axonopodis pv. citri and only one site was free of citrus canker disease. Total cellular DNA was isolated from freshly growing aqueous bacterial culture...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...ir potential role in the pathogenesis of rheumatoid arthritis (RA). Due to the confounding results, reported in several studies, we performed the systematic literature search on IL-6 -572 G/C polymorphism (rs1800796 SNP) and assessed its association with RA in the overall world population, and in the subgroup analysis of Asian and Caucasian populations. The results showed that in overall population, IL-6 -572 G/C shows a significant association towards increas...

Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3 and Waqas Raza1*

Influence of Resistance Inducers on Nitrogen, Phosphorus and Potassium Contents of Susceptible Chickpea Cultivars after Inoculation with Ascochyta rabiei
...f inoculation with plant pathogen in plants pretreated with chemicals. There was no significant change in plants treated with the plant extracts except with extracts of A. indica. Chemically increased N, P, and K were highest in cultivar C-44 followed by Pb-91 and Bittle-98. A similar trend was observed with plant extract treated plants, with the greatest effect observed with A. indica extracts. The results indicate that chemicals reported to induce resistance...

Shishir Sharma* and Laxmi Prasad Joshi

Current Insights on Stemphylium Blight of Lentil with its Management Strategies
...20c, and cloudiness. The pathogen mainly persists in crop debris and occasionally in seed during the offseason. Mapping QTLs by utilizing wild varieties and landraces may be used to develop disease-resistant varieties which it is the most efficient, cost-effective, and environmentally sustainable technique. Integrated disease management approaches along with Omics and weather prediction tools may be crucial in avoiding severe SB outbreaks. A comprehensive revi...
Ashara Sajid1, Muhammad Usman Ghazanfar1, Saeed Rauf 2, Zahoor Hussain3, Salman Ahmad1 and Yasir Iftikhar1,*
... and to confirm greening pathogen with two primer pairs, which amplified the specific DNA (~1160bp) and βoperon (
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...rmance, immunity against pathogenic microbes for production of quality meat.
...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
... is an opportunistic pathogen and the leading cause of nosocomial infection worldwide. This bacterium produces enzymes known as extended spectrum β-lactamases which render broad spectrum cephalosporins and penicillins inactive. This study reports antibiotic susceptibility pattern, multiple antibiotic resistance (MAR) index and prevalence of extended spectrum β-lactamases among clinical isolates of P. aeruginosa collected from tertiary care...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...l;">The ability of entomopathogenic fungi to be applied for pest control in field applications is often hampered by negatively active abiotic factors including high temperature, desiccation and UV irradiation. Selecting isolates with high UV tolerance and virulence is important in improving the efficacy and utility of fungal insect pathogens as insect biological control agents for use under field conditions. UV-irradiation o...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
...inarum,is a host specificpathogenic bacterium of fowl typhoid, one of the most important diseases of poultry that increases the death rate and reduction in eggs production. Bacteria forms the complex structural colonies enclosed in a sticky matrix known as biofilm. Various antimicrobial approaches used to treat gastrointestinal infections are usually ineffective due to biofilm formation. The purpose of this study was to (1) compare biofilm formation of three <...
Laura Alejandra Arriola-Mosqueda1, Yadira Jiménez-Lara2, Eugenia del Carmen Prieto-Avella1, Carlos Ricardo Cruz-Vazquez3, Roberto Montesinos-Matias4, Mauricio Valencia-Posadas2, Ana Martha Cruz-Avalos5, Jaime Molina-Ochoa6,7 and César Andrés Angel-Sahagún2,*
...ae. We evaluated the pathogenicity on the eggs and larvae of all tested strains of R. sanguineus at day 3 and 7. The LC50 of four strains of M. anisopliae was determined by Probit analysis, and a chi-square test was used to determine the relationship of LC50 with variable radial growth and germination at 18 and 24 h. Germination at 18 h and 24 h ranged from 83.8–92.5% and 89.5–100%, respectively. The average ...
Saba Shabeer1, Riffat Tahira2 and Atif Jamal3*
 
... the world, amongst them pathogenic species can attack plants at different stages causing considerable damage amounting to millions of rupees. One of the plant pathogenic fungi is Fusarium spp. Fusarium species are very well-known soil-inhabiting fungi that cause many economically important diseases of crops.Many species are included in the Fusarium genus, which are not only
Ryan Septa Kurnia1* and Radiana Dhewayani Antarianto2
 

...led substances including pathogens and allergens. Current research about mechanism of disease and drug development is conducted in 2D cell culture or in animal models. The 2D cell culture models poorly imitate the condition in vivo and provide limited utility due to mimic tissue physiology in multicellular organisms. Although animal models can be used as pre-clinical tools for new-agent screening prior to clinical testing, there is a growing awareness o...
Hafiz Tassawar Abbas1*, Tamoor Khan1, Ghulam Khaliq2, Muhammad Aqeel Sarwar3, Muhammad Rashid4, Intazar Ali5, Muhammad Abuzar Jaffar2, Ghulam Ali Bugti6 and Muhammad Waseem4
 

...to prevent the extent of pathogen

...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
... (MF543123) against fish pathogen Pseudomonas fluorescens. Antagonism by cross streak was observed in ten isolates out of total twenty. Six isolates showed better inhibition by well as well as disk diffusion methods with maximum growth inhibition zones i.e., 15 and 17 mm by Sphingomonas sp. AsCh-P3 and Bacillus aerius AsCh-A7 respectively. Six probiotic bacterial isolates of known enumeration (C.F.U.ml-1) showed growth an...

Uwem E. George1, Oluwadamilola A. Arowolo2, Oluseyi A. Olayinka3, Ijeoma M. Ifeorah4, Temitope O.C. Faleye3,5, Bolaji Oluremi6, Arthur O. Oragwa7, Ewean C. Omoruyi8, Emmanuel E. Udoh9, Oluwadamilola G. Osasona1,10, Emmanuel Donbraye11, Olufunke P. Adeniji12, Olubusuyi M. Adewumi3,13* and Johnson A. Adeniji3,13,14

...e selection of resistant pathogens and high levels of Antibiotic Resistance Genes (ARGs). We examined Nigerian Chimpanzees in Boki Afi Mountain Wildlife Sanctuary, Cross-River State, Nigeria, to detect ARGs. Faecal samples from 15 Chimpanzees in pristine enclosures of Non-Human Primates in the Wildlife Sanctuary were analyzed. All faecal samples were pooled, then resuspended in phosphate-buffered saline. Subsequently, nucleic acid was extracted from the suspen...

Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3, Waqas Raza1* and Mahmood Ahmad Randhawa4

...t took place during host-pathogen interaction have been poorly investigated. Although photosynthesis disturbance and carbon metabolism re-programming have been studied yet, there is scary of data on plant amino acids after induction of resistance. Here we investigated the amino acid contents in induced and un-inoculated and invaded region after induction of resistance. The quantities of methionine, iso-leucine, leucine, tyrosine and phenylalanine contents in t...

Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*

...blished bacteria’s pathogenesis, but still, the literature about the role of bacteria and its immunogens on the demonstration of clinical signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane pro...

Salma Javed and Samreen Khan*

...mstances to control many pathogenic soil-borne fungi because this is the first study conducted on the subject experiment in Pakistan.

...

Muhammad Arslan Ibrahim1*, Aneeqa Aleem2, Farkhanda Manzoor2, Shahbaz Ahmad1, Hafiz Muhammad Zahid Anwar1, Talia Aroob2 and Mansoor Ahmad3 

...se of 4µl of entomopathogenic NPV suspension containing 4×106 polyhedral inclusion bodies (PIB) was tested as a mortality factor against the late larval instars of S. frugiperda when natural mortality tends to decrease after each successive instar. The single-sex method was adopted in the construction of the life table. The ratio of mortality in the late instars (3rd to 5th) was greater than the early instars because of entomo...
Arsalan Rasheed1, Tahir Usman2,*, Sadaf Niaz1, Irfan Khattak2, Saira Gul2, Nawab Ali1,Naimat Ullah Khan2, Hazrat Ali2, and Mian Saeed Sarwar2
...re not considered highly pathogenic to humans until severe respiratory syndrome broke out in China during 2003. Middle East Respiratory Syndrome, Severe Acute Respiratory Syndrome, and novel Corona Virus Disease (nCOVID-19) or Severe Acute Respiratory Syndrome-2 have increased the interest in this viral family. The Coronavirus-19 is a novel strain that was recently discovered in Wuhan (China) in December 2019. Studies revealed that reservoir of all the three f...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1

Qaiser Shakeel1*, Rabia Tahir Bajwa1­, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4

...llium digitatum, a plant pathogenic fungi responsible for ginger soft rot. Petri dish were used to conduct bioassay through poisoned food technique with triplicates. The crude extract of all the plants showed a better inhibitory effects on the pathogen’s (P. digitatum) mycelial growth rather than fungicides. Among all the plant extracts, the best result was showed by garlic extract with 87% of mycelial growth inhibitio...
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...sk of contamination with pathogenic strains of Staphylococcus aureus. The current study was designed to investigate prevalence of pathogenic strains of S. aureus, assessment of risk factors, and in-vitro antibiogram of non-biofilm producing S. aureus (nbpSA) and biofilm positive S. aureus (bpSA)from mastitic goats. The purposive sampling technique was applied to collect n=200 milk samples f...

Muhamamd Rizwan1*, Muhammad Arshad2, Muhammad Kashif3, Aneela Zameer Durrani4, Asghar Abbas5, Tanveer Ahmad6, Muhammad Nadeem7 , Kinza Khan8

...antibiotic resistance in pathogenic bacteria, enhance their pathogenicity and can survival in host body. 
 
Novelty Statement | CRISPR Cas system is an integral part of prokaryotic immune system that provides protection against viral infection and involved in genome editing, bacterial virulence and antibiotics resistance.
...

Md. Mukthar Mia1 and Mahamudul Hasan2

...e. Distressingly, such a pathogen is currently endemic in Asian nations, including India, Bangladesh, Sri Lanka and China. Thus, the literature review encompassed comprehensive knowledge concerning contemporary disease occurrence with causes and transmission imperative for management practice. Besides, the study converged on afresh advanced treatment procedures, vaccine progressions, and public awareness efforts, which can be a baseline for the policymaker, ve...

Gulnaz Parveen1*, Naila Mukhtar2, Shumaila Irum3 and Nain Bukhari4

...tification of the causal pathogen is important before appropriate treatment can be made to control the pathogens. Therefore, In the present study, Alternaria alternata, Alternaria, Rhizopus, Mucor, Aspergillus niger, Aspergillus flavus, Cladosporium, Botrytis cinerea, Fusarium moniliforme, Drechslera, Penicillium, and Geotrichum candidum were identified among various vegetables including potato, carrot, okra, eggplant, turni...
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...de, as it is an emerging pathogen among immunocompetent individuals. There are no epidemiological data on the prevalence of this fungus in donkeys. The current research was conducted to investigate the possible role of the Egyptian donkeys in the epidemiology of such pathogen. Bacteriological analysis of nasal swabs of 52 diseased and healthy donkeys at different localities in Egypt revealed that the overall occurrence of

NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2

... cultivation in specific pathogen free (SPF) embryonating chicken eggs
(ECE),haemagglutination (HA), haemagglutination inhibition (HI) and mean death time
(MDT)used in the investigation to reveal virus identity and pathogenicity. Both NDV
isolates were found to be velogenic strains. Real time reverse transcriptase polymerase
chain reaction (rRT-PCR) used as a confirmatory techn...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...e utilized.

...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...div>
transmissible pathogens of citrus including prokaryotic pathogens,
DNA and RNA viruses and viroid provides a new approach to virus
and virus like detection and identification. The method provides a
pure preparation of un-degraded RNA and DNA in high yield and
can be completed within 2 h. It is particularly useful for processing
large number of samples and...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...ioc membrane of specific pathogen free (SPF) embroynated chicken egg (ECE) showing the characteristic pock lesions for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV isolates were 2 and 2.75 when neutrized by FPV hyperi...

Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1

...herpesvirus (EHV)-1 is a pathogen of horses, well known for its ability to induce abortion and nervous system disorders neonatal foal pneumonitis, highly contagious among susceptible horses, with viral Transmission to cohort animals occurring by inhalation of aerosols of virus-laden respiratory secretions. Extensive use of vaccines has not eliminated EHV infections, and the worldwide annual financial burden from these equine pathogen

Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2

...tudy. Ala149, which is a pathogeneicity marker for interferon antagonism was found in the studied indicating capability of these viruses to transmit between different avian species. PL motif ESEV was found in 6 isolates from chicken and ducks circulating in such population whereas 17 viruses have ESKV similar to those reported in human in 2006. Based on NS1 sequence analysis of the H5N1 Egyptian viruses, neither geographic nor species signature were reported. ...

Rafia Ahsan1, Saif Ullah1, Ijaz Yaseen1, Faisal Sohail Fateh1, Muhammad Fayyaz1, Shahzad Asad1, Atif Jamal1, Muhammad Sufyan2 and Muhammad Zakria1*

...ae (Xoo) through PCR and pathogenicity on susceptible host. The results indicate that BLB is continuously present in rice growing areas of Pakistan specially in Basmati area of Punjab for last many years. There is need to introduce resistant Basmati varieties to reduce the inoculum level and yield losses.

...

Salama M. El-Saghir1, 2

... phytoplasma as a second pathogen in the
trees was PCR tested using the universal primers for phytoplasma detection, P1/P7, in the first step.
The PCR products were re‐amplified with nested‐PCR to verify phytoplasma incidence using the
nested primers R16F2/R2.
Results: DAS-ELISA confirmed the presence of PNRSV in the tested peach trees. For phytoplasma
incidence, the universal primers P1/P7 amplif...

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

... important but difficult pathogen to control in
poultry. Vaccination might reduce the risk of bird infection virus transmission by reducing virus
shedding. Challenge of vaccinated birds with AI vaccines under strictly controlled condition with
virulent HPAI virus may also be used to predict flock response to AI infection. The reduction in
challenge virus replication can be quantified using classical virus isolati...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...was isolated in specific pathogen free (SPF) embryonated chicken eggs ECE), chicken embryo kidney
cell (CEK) and in Vero cell line and was confirmed for IBV detection by Reverse Transcriptase Real
time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done
by generating a standard curve by real time PCR.
Results: The results revealed that the propagated virus in CEK showed the...

Abdou Nagy1, Fatma Abdallah1, Kareem Sweed2, Ahmed Salama3, Mohamed Omar4

...ne of common respiratory pathogens affects
poultry and can cause economic losses, particularly when accompanied with secondary pathogens.
Aim: This study aimed to perform seroprevalence of aMPV for non-vaccinated chicken and duck
flocks from six provinces of Egypt (Ash Sharqiyah, Ismailia, Qalyubia, Menufia, Sohaq and North-
Sinai) and determine its economic effect.

Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid

...lly isolated on specific pathogen free embryonated chicken eggs (SPF-ECE). Additional
RT-PCR testing and re-isolation of ARV from ECEs of a breeder flock of a history of uneven growth
and/or arthritis revealed ARV infection in six (6/60) examined ECEs.
Conclusion: These results indicated the incrimination of ARV infection in some arthritis infections
beside the low food conversion rate of chickens within Sharkia ...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...>
spread of highly pathogenic H5N1. Determination of the optimal antigen content of avian influenza virus
vaccines is urgent to reach protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated wit...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...nsmission of blood borne pathogens. As Pakistan lacks medical facilities. the government has to get testing kits and primers from other countries. Only few quarantine centers are available and there is shortage of drugs, beds, trained doctors and paramedical staff. Unfortunately, no vaccine is available yet so the only management strategy is the prevention of infection by wearing masks and following social distancing. Cleanliness, hygiene and quarantine measur...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...v>is a highly contagious pathogen, mainly transmitted by inhalation of aerosols of virus laden respiratory
secretions especially with high population during equestrian events. Reactivation of virus in nonsymptomatic,
latently (silently) infected horses is the main cause of circulation of such virus in horses.
Methods: In the present study, molecular characterization of circulating EHV-1 viruses among horses
of eq...

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

...on
levels of pathogenesis related genes, chitinase, PAL1 and LOX1.
Conclusion: In cucumber, treatments with artificially generated wind and root colonizing F. equiseti
GF19-1 or its CF elicited induced resistance against CMV infection, leading to a restriction of
pathogen propagation and disease development.
...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
...ramuscularly in specific pathogen free and commercial layer chicken, the injection was
repeated as a booster vaccination one month after the first vaccination. Humoral immune response was
measured by HI for both H9N2 and LaSota viruses weekly for one and a half month after the booster
vaccination, then monthly until the end of duration. Challenge trials were carried out after a month
from the first vaccination, a...

Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2

...ramuscularly in specific pathogen free and commercial layer chicken, the injection was
repeated as a booster vaccination after a month from the first vaccination. Humoral immune response
for IBV was measured by ELISA weekly until about one month after the booster vaccination then
periodically till the end of duration. Challenge trial for both IB strain was repeated after a month from
the first vaccination and aft...

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

...increased expressions of pathogenesis related genes PR1 and PR2 relative to the control plants.
Conclusion: PGPF isolates of GP17-2, GF19-1 and SKT1 elicited induced resistance against BYMV infection and could be used as a promising strategy to control BYMV.
...

Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1

...expressions of different pathogenesis related genes (Chitinase, β 1-3 Glucanase, PR1, POX, PAL and LOX) explaining the role of these genes in the mechanism of induced resistance against PRSV by GU23-3.
Conclusion: GU23-3 could be considered a new and alternative strategy to control PRSV infection.
...

Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3

...rtant potato
pathogenic virus worldwide. PLRV belongs to genus Potyvirus, transmitted by aphids and
widespread in most potato growing areas globally, that causes huge losses in potato crop
production. PLRV management became a challenge due to the economic importance of potato
(Solanum tuberosum L.), the fourth largest staple crop in the world. Our research was aiming
to manage PLRV In-Vitro.
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...rent known commensal and pathogenic microorganisms that
have been reported to replicate in ticks (e.g. Staphylococci, streptococci ) In addition, the analysis
revealed a LSDV sequence output that was nearly equal to sequence output of different bacteria
known to replicate in ticks.
Conclusion: The modified RT-PCR assay used to detect the polyadenylated LSDV DNA polymerase
mRNA in combination with data...

Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder

... sugar beet plant by the pathogen, as well as, to determine some morphometric parameters
such as upper and lower epidermis thickness, midrib thickness, blade thickness, palisade tissue
thickness, spongy tissue thickness, height and width of the vascular bundle.
Results: Phylogenetic analysis results indicated that SEA-CMV-isolate under study (acc. no.
MT491996) closely related (99.9%) to Beet-EG-CMV-isolate (JX82...

Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski

...ost important food borne pathogens. Since the start of the 21st century C. jejuni is the leading cause for food borne enteritis. Another point of attention is the change in the antimicrobial resistance of this microorganism towards some critical antimicrobials used in the human and veterinary medicine. In this study samples were taken from three points in the broiler meat production (farm, slaughter line and cold storage of the meat before shipping to...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1,  Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...nalysis. Twenty specific pathogen-free (SPF) chickens were divided into two groups as control and treatment. The chickens of treatment group were inoculated with MDV, and blood samples of two groups were obtained at 14 and 22 days post-infection (dpi) for RNA extraction. Transcriptome analysis was performed to explore the immune responses of chicken erythrocytes to MDV infection at 14 dpi and 22 dpi. In total, 129.6 million reads of two groups at two time poin...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Mehwish Iqtedar2 and Javed Iqbal Qazi3,*
... with well-renowned fish pathogenic bacterium Vibrio anguillarum. Positive control-C2 as well as experimental groups was challenged with V. anguillarum intraperitoneally. Probiotic treated feed with antibacterial potential against V. anguillarum was administered to experimental fishes in different groups; G1 (soon after challenge for 30 days), G2 (for 15 days prior and 30 days after challenge) and G3 (for 15 days prior to challenge). The s...
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
...tant zoonotic tick-borne pathogen transmitted by the tick Rhipicephalus sanguineus (Latrielle) that causes canine monocytic ehrlichiosis (CME) in dogs. We have investigated the prevalence of R. sanguineus and E. canis infection in pet dogs in Wenzhou, China. Serum samples were obtained from animal hospitals were examined for antibodies by commercial rapid in-clinic ELISA kits. Ticks were collected from dogs and molecular detection methods ...

Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2

...ar, bulb and root fungal pathogens that badly destroy onion crops, greatly reducing its yield and quality. Fusarium oxysporum is a very common soil-borne fungus that causes basal rot onion and it has the ability to survive in the soil for many years without having a host. Many fungicides are available in the market to control the basal rot of onion which contain hazardous material that continuously destroys the environment and human health. The objective of th...
Iram Liaqat1,*, Safdar Ali Mirza2, Sumera Sajjad3, Shaukat Ali1,*, Muhammad Faiz Qamar4 and Ikram Ul Haq5
... gram-negative bacterial pathogens use flagella to invade foreign surfaces, host tissues and substrates. Flagellar biosynthesis and function in Salmonella typhimurium isregulated by >50 genes. Bioinformatics analysis of flagellar assembly in S. typhimurium identified several conserved structural elements. In this study, FliI a flagellar protein required for flagellar assembly and involved in a specialized protein export pathway was cloned and ...
Waqar Ali1,3*, Muhammad Yaqoob2, Abida Arshad1, Guifu Dai3,Safir Ullah Khan1, Muhammad Arshad Malik4, Imran Ullah1 and Ihsan Ullah1
...was found most prevalent pathogen that causes mastitis in goats. The overall prevalence of goat mastitis was recorded (11.67%). Risk factor like age, lactation stage, parity number and unhygienic condition showed statistically significant association with the prevalence of mastitis in beetal goats. Antibiotics sensitivity profiles of investigated bacteria showed that these were most sensitive to gentamycin, ciprofloxacin and amoxicillin.
...

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

... against selected common pathogenic bacteria (Esherichia coli, Salmonella typhimurium SJW1102, and Staphylococcus aureus ). According to the study, the DgS and DSS solutions of egg-sac silk exhibited no inhibitory potential by either of the two methods. However, application of web silk using the DSS treated discs showed no growth inhibition compared to the DgS treated discs, where increased inhibition zones were observed against all three bacteria. This study ...
Anwar Khan1, Fareeha Nosheen2,4, Bushra Tabassum2*, Olawale Samuel Adeyinka2, Khurram Shehzad3, Naila Shahid2, Arif Muhammad Khan4 and Idrees Ahmad Nasir2
...n viral transmission and pathogenesis. RNA interference or post transcriptional gene silencing is a regulatory conserved mechanism that uses small interfering RNAs to control the expression of desired gene by inhibiting mRNA transcription. The present study aims to investigate the potential role of different siRNAs in down-regulating the mRNA expression of potato virus X in cell culture assays. Three specific siRNAs against CP-PVX gene were designed in which s...

Mukhtar Iderawumi Abdulraheem1*, Muhammad Ihtisham2*, Abiodun Yusuff Moshood3, Nawab Khan4, Muhammad Owais Shahid5, Shafiq Hussain6, Kumail Abbas6 and Fawad Zaman7

...eral elements along with pathogens, pests, and very importantly viral diseases, restrict the belief of these intended goals. The current experiment assessed the effects of mulching materials and weeding regimes on the severity and incidence of not unusual viral diseases on okra without fertilizers/chemicals application. The treatments were (3×4) factorially designed and fitted into RCBD in field conditions. Weeding consisted of four levels i.e. no weedin...
Madiha Nawaz and Shoaib Freed


... local isolates of entomopathogenic fungi on 2nd nymphal instar of P. solenopsis under laboratory conditions by immersion method. Three entomopathogenic fungi; Beauveria bassiana (isolates Bb-01, Bb-08), Metarhizium anisopliae (isolates Ma-11.1, Ma-2.1) and Isaria fumosorosea (isolates If-2.3, If-02) showed percent mortalities of (61.0%, 85.0%), (78.0%, 56.0%) and (52.0%, 54.0%) with LC...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

... with dried fish related pathogen to animal and humans.

...
Ikechukwu Eugene Onah*, Ifeoma Esther Aniaku, Jude Ifeanyi Okwor and Ada Sylvester Haruna
...ow through which disease pathogens could be transmitted. The plasma biochemistry parameters of C. albus determined in this study are higher in females than in the males but the mean plasma biochemistry parameters are not significantly different between the sexes (p > 0.05). A total of 388 crows comprising 276 (71.13%) and 112 (28.87%) from food and water sources respectively were recorded in the study. The population of pied crows in food sources wer...

Samreen Khan*, Salma Javed, Tabassum Ara Khanum, Nasira Kazi

...stify;">Abstract | Entomopathogenic nematodes are one of the perfect biological control agents against many insect pests all around the world. In this regard, to collect data about entomopathogenic nematodes regarding their presence and distribution in remote areas such as one of the southern district Lakki Marwat of Khyber Pakhtunkhwa province. Extensive surveys carried out in the months of October 2019 to February 2020. To...

Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2* 

...da is the main bacterial pathogen causing respiratory manifestations (snuffles) in rabbits with significant economic losses and poor prognosis in Egypt. So, this study aims to detect and identify P. multocida isolated from rabbits by molecular techniques. To establish this goal, lungs, liver, and heart (50 for each organ) were collected from 50 diseased rabbits (suffering from respiratory signs) from backyard rabbits at different localities in Sohag governora...
Ardi Prasetio1*, Christina Maria Sri Lestari2, Sutaryo Sutaryo2, Manar Fayiz Mousa Atoum3,4Muhammad Zahoor5, Asma Nisar6 and Muhannad Illayan Massadeh7
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*

...> (ETEC) is an important pathogen causing diarrhea of newborn or weaned piglets, which seriously harms animal husbandry and causes huge economic losses. The pathogenicity of E. coli is closely related to its virulence factors, which are strictly regulated in vivo, and the quorum sensing system is involved in this process. To study the effect of quorum sensing signal molecule acyl homoserine lactone (AHL) on the...

Altaf Hussain1*, Muhammad Saad Ashraf3, Asim Shamim1, Mohsin Nawaz1, Arslan Mehboob2 and Urooj Fatima2

Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2 

...well as, to assess their pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Salmonella with the acquisition of shish-tawook (14%). On the other hand, 100 hand swabs that were assembled from food handlers working in the same places yielded 13 Salmonella isolates, at the time 4 isolates wer...

Rehman Shahzad1, Saba Irshad1*, Malik Saddique Mehmood1 and Faisal Amin2

...uting to the increase in pathogenicity of the circulating H9N2 virus.

...

Mustafa Ozan Atasoy

...le tool owing to its non-pathogenic characteristic, relatively higher transgenic capacity, and enabling the DIVA strategy. Numerous bivalent and multivalent HVT constructs have been generated by various methods and some of these constructs have been made commercially available so far. The efficacy of HVT-vectored vaccines has been assessed either individually or in combination with other vaccine formulations; hence, the optimal competence of these vaccines aga...

Xiaoli Cai1,2, Saeed Ahmed3, Zhixin Lei1,*, Jianping Wu1,* and Taolei Sun1,2,*

... justify;">Intracellular pathogenic bacteria cause chronic, persistent and latent infections. The treatment for intracellular infection faces many challenges, such as targeting bacteria with antimicrobial drugs and resistance to multidrug (MDR). The present study delivers a possible elucidation to this problem via Nano Drug Delivery. Nanomaterials are relatively small as compared to ordinary antibiotics. The nanoparticle has unique physical and chemical proper...

Irshad Ahmad1,2*

...l agents including entomopathogenic fungi, nematodes as well as adopting the regulatory methods of domestic and international quarantine. This paper provides updated information about IPM, which look like a promising paradigm that can be adopted for the control of red palm weevil.

...

Amjad Ali Channa1, Mansoor Tariq1*, Zaheer Ahmed Nizamani1, Nazeer Hussain Kalhoro2 

...re highly contagious and pathogenic viruses that caused severe disease in poultry and are potentially zoonotic. Since last decade the outbreaks of AIVs have been increased globally including Pakistan. Therefore this study was conducted to determine the prevalence of avian influenza H5, H7 and H9 viruses in broilers at Karachi, Pakistan. A total of 1920 samples, including each of 960 blood samples and tracheal swabs were collected from broilers and tested throu...

Zheng Tan*, Li Li, Yuxin Song, Meirong Tian and Fan Jia

...s play a key role in the pathogenesis and can be used as indicators for clinical risk stratification of CHD.

...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...infection is a core aetiopathogenetic factor in the development of leukaemia in cats. The fundamental methods for such pathologies diagnostics, along with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic ca...

Hams M.A. Mohamed1, Mona A. El-Zamkan2* 

...s comprises a variety of pathogenic and commensal bacteria that show a surprising capacity for adaptation to new hosts and antimicrobial resistance, resulting in the spread of infection all over the world, leading to huge health loss. A total of 100 raw milk samples collected from small scale producers, farmers and markets at Qena city, Egypt, were examined for the presence of Streptococcus species. The preliminary identification was confirmed by using VITEK&r...

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...ontamination by numerous pathogens concerning Salmonella species. The current study aimed to investigate the prevalence of Salmonella contributing contamination in chicken samples that were collected from broiler farms and slaughterhouses using conventional culturing, biochemical, and serological identifications versus molecular detection. A prospective study was designed to last for six months from March 2021 to the end of August 2021. A total number of 126 c...

Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1 

... of some diseases. Entomopathogenic fungi, particularly Beauveria bassiana and Metarhizium anisopliae, and botanical oils have shown potential as synthetic insecticides alternative for house fly control. In the present work, local isolates from both fungi as well as their mixtures with essential oils were evaluated against house fly larvae under laboratory conditions. Batches of house fly larvae (25 individuals per replicate and 4 replicates per dose of fungi)...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
...resistance in food-borne pathogens. Antibiotic resistance is setting the achievements of present day medication at chance. Therefore, in this study 300 chilled broilers carcasses were collected from different poultry markets in Ismailia City, Egypt to evaluate qualitative, quantitative and tetracycline levels. Qualitative determination of tetracycline residues was done using rapid tetracycline test kit. Second aim of the study was fined a safe way to control t...

Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad 

...omorphology and selected pathogenic bacteria in broiler chickens. A total of 160 day-old Ross chicks were assigned to four groups, containing 0, 0.5,1 and 1.5% GA along with basal feed for 42 days. A completely randomized design (CRD) was followed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, ...

Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1* 

...is caused by serovars of pathogenic Leptospira. A pilot study was conducted in some of the dairy farms located in Telangana and Andhra Pradesh, endemic states of India, during 2017 to investigate the seropositivity of bovine leptospirosis in dairy animals and associated risk factors at the farm level. The semi-structured questionnaire was designed as per the literature and used to collect leptospirosis associated risk factors along with serum samples from eigh...

Palwasha1, Siraj-ud-Din1 and Muhammad Fahim2*

... that either the pest or pathogen or both might have developed pesticide-resistances. We conclude that i. lack of knowledge about pests and diseases, ii monocropping and use of decade’s old cultivars, iii. lack of awareness about diseases, their ecology and epidemiology, iv. lack of knowledge about good phytosanitary practices and v. inability to choose the right pesticide/fungicide for better management are some of the contributing factors behind peach ...

Evrim Sönmez

...ing the effects of entomopathogenic fungi on pests in recent years, researchers have also focused on fungi in fighting pests. These fungi, which are sold as bioinsecticides in the market, are generally preferred by producers who want to engage in organic or environmentally sensitive agriculture. Although there are a large number of sources in literature on the mortality rates of Beauveria bassiana’s A. obtectus, studies on the effects on the biology of t...

Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5

...ne to identify potential pathogenic sequence variants. CEP patients were identified using successive clinical tests. Blood samples of patients were collected and processed for genomic DNA extraction followed by Sanger sequencing to identify pathogenic mutations in UROS gene. Sequence analysis revealed a pathogenic missense mutation (c.935T>C [p. L237P]) in the exon 10.The sequence was f...

Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim

...esistance genes to other pathogenic bacteria through horizontal and vertical gene transfer which could make them superbugs and non-curable through antibiotics. Hence, proper hygienic conditions should be maintained in order to stop the spread of these bacteria to human population.

...

Mona M. Osman1, Kamelia M. Osman2, Manal Abu Elmakarem Mohamed1, Mahmoud E. Hashad2, Jeeser Alves Almeida3, Alaa Saad4, Octavio Luiz Franco5,6, Heba N. Deif 2* 

...actors implicated in the pathogenicity of Mycoplasma arginini (M. arginini) and Mycoplasma ovipneumoniae (M. ovipneumoniae). It shed light on the current knowledge gap in the role of two potential virulence determinants viz H2S production and catalase activity, for their possible roles in biofilm formation, antibiotic resistance and hemolytic activity of M. ovipneumoniae. The recovered sheep isolates of M. arginini and M. ovipneumoniae were examined bacteriolo...

Basanta Kumar Adhikari1*, Deepak Subedi1*, Sumit Jyoti1, Krishna Kaphle2, Chet Narayan Kharel3, Doj Raj Khanal4

...ntial circulation of the pathogen to other parts of the country warranting the concerned authorities and farmers to be vigilant for keeping this disease at bay.

...

Rasha Ali Taha Hamza1, Atef Saad Osheba1, Hassan Mohamed Sobhy2, Sahar Hussein Abdalla Hekal2* 

...lipid oxidation and many pathogens, which carry the risk for human health. The aim of this work is to determine the antioxidant and antimicrobial activities as well as total phenolic and flavonoids contents in both water and ethanolic extracts of galangal and sumac extracts. Also, the effect of previous extracts on quality attributes of beef burger was evaluated. The ethanolic extracts of both galangal and sumac were shown to have significantly higher total ph...

Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3

...o minimize the attack of pathogen. Tomato leaves infected with early blight symptoms were collected from Lahore. Pathogen was isolated on PDA and its morphological characters were studied. All the characters used for identification of pathogen confirmed the culture as Alterneria solani. Ganoderma lucidum was collected from University of the Punjab, Lahore associated with dead oaks of Dalbe...

Eman Alsayed Hammad and Atef Mohamed El-Sagheer

...the most important plant pathogens in most Pomegranate cultivation regions. By studying the structure of the plant-parasitic nematodes community inhabiting the rhizosphere of Pomegranate fields, it was found that Meloidogyne is the most common genus and harmful one. So, the efficacy comparative of Chitosan, Marjoram emulsion oil, Trichoderma asperellum, and Vermicompost as alternative eco-friendly control agents for root-knot nematode M. incognita infected Pom...

Jiang Wu*

...cinale against foodborne pathogens. A total of 133 retail meat products were randomly purchased from different supermarkets. All samples were isolated and identified by conventional methods as Staphylococcus aureus, Salmonella, Escherichia coli and Listeria monocytogenes. Different bacteria were respectively inoculated into LB medium, the OD600 was adjusted to 0.5, and the initial bacterial concentration was adjusted to about 5×108 CFU/mL. T. officinale ...

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...ered to be a significant pathogen of domestic and wild canids and has spread worldwide. This study was conducted on fecal samples from suspected parvovirus dogs were taken from different provinces in north Iraq for PCR testing, and 76% of such samples were confirmed to be positive. Sequence analysis showed two CPV-2c, six new CPV-2b, and ten new CPV-2a variants. In the CPV-2 studied, several unique and existing mutations have been discovered, indicating the em...

Haroon1, Chen-Xu Ye1, Yu-Xin Li1, Hong-Xin Zhang1, Qing Liu1, Xiao-Hong Su1,2,3 and Lian-Xi Xing1,2,3,*

...iveness, protection from pathogens and parasites.

...
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
...8ab△hcp1/phcp1), their pathogenic differences were compared, and the function of hcp1 was discussed from the aspects of growth curve, biofilm formation, adherence, invasion, cytotoxicity, and so on. The results showed that hcp1 of the T6SS did not influence bacterial growth and biofilm formation. In F18ab△hcp1, bacterial motility, adherence and invasion towards the intestinal porcine enterocyte cells (IPEC-J2) were reduced significantly, and the cytotoxici...

Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5

...biotic stresses, certain pathogens and nematodes, market devaluation and access, urbanization, poor gardening, poverty climate change, small landholdings, exogenous factors and managerial decisions, etc. So, to counter these fruit yield declining factors the use of advanced molecular markers is a handy option for the improvement of the fruit germplasm in Pakistan. Additionally, they also have diverse applications in the area of genetic diversity, varietal iden...

Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1* 

...ns are caused by various pathogens, including bacterial and viral agents. In Egypt, the prevalences of avian influenza virus (AIV), infectious bronchitis virus (IBV), and Mycoplasma spp. are high. Thus, co-infection with one or both viruses and Mycoplasma gallisepticum (MG) or Mycoplasma synovaie (MS) is thought to play an important role in the clinical severity of respiratory infections. In this study, we conducted a passive surveillance study for MG and MS a...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
...ransmission of foodborne pathogens. Aeromonas spp. is one of the opportunistic microorganisms, that normally inhabits aquatic environments such as fresh, and marine water bodies. Aeromonas spp. might cause food-borne gastroenteritis that might be complicated to cause septicemia, meningitis, endocarditis, and osteomyelitis with high mortalities in immune-compromised persons. This international collaborative research was conducted to investigate the prevalence o...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
...microbial effect against pathogenic bacteria while, it kept probiotic bacteria alive. The animals fed on HFD showed elevated lipid profile, leptin, oxidative markers and low level of testosterone and the epididymal Spermatozoa had low sperm viability and higher abnormalities than control group. Feeding the microcapsules (G2,G3) improved the animals’ health condition. Animals in G2 showed good spermatozoa profile while, those fed SP (G3) showed low sperm ...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
... all over the world. The pathogenesis of ND in quails is still poorly understood. The present study aimed to investigate the natural occurrence and pathological changes of ND in quails in Egypt. The diseased quails were collected from eleven flocks in Egypt exhibited respiratory, nervous signs and whitish green diarrhea in addition to egg abnormalities and lower production with mortalities. No characteristic gross lesions were observed except heamorrhages in b...

Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2** 

...d with other respiratory pathogens. In the current study AIV H9 was identified among broiler chickens in Luxor governorate in 2016. Five hundred (n= 500) samples were collected from fifty farms (5 tracheal and 5 cloacal pools from each farm) have been examined by real time RT-PCR for the prevalence of AIV H9. The results revealed that ten farms (20%) were positive for AIV (H9N2). Three isolates with high CT values have been selected for complete sequencing of...

Rizwana Abdul Ghaffar* and Kamil Nadeem

...yers. This causes severe pathogenicity in the intestine as a result the pigeon shows signs and symptoms of illness. Pathological studies of tissues from infected intestine reveal several changes from the normal architectural organization of tissues. The most common findings were massive congestion, cellular infiltration, atrophy, hypertrophy and dystrophy of cells and disintegration of cellular layers, fibrosis, necrosis and dilation and proliferation of blood...

Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2

...serotype 078: K80:H9) at pathogenicity amount (9.4 x 105 cfu E. coli per bird) was done and morbidity and mortality rate was also recorded. The significantly minimum ammonia (ppm) was recorded in CRH (30.53±0.77); however, the maximum ammonia (ppm) was recorded in RH (49.78±0.42) with that compared to SD (33.42±0.25) and RS (43.22±0.56). Similarly, the mortality rate due to colisepticemia was recorded minimum in CRH (68.75%), howeve...

Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3

...cterial activity against pathogenic bacteria such as E. coli, Pseudomonas, Listeria inokua and Klebsiella pneumonia. Base on the phylogenetic analysis, the bacteria isolated were closely related to Limosilactobacillus fermentum, a probiotic bacteria candidate.
 
Keywords | Lactic acid bacteria, Limosilactobacillus fermentum, Antimicrobial, Probiotic
...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...of postpartum. The exact pathogenesis of COD is still not confirmed, but the abnormal neuroendocrine reflex of hypothalamic pituitary dysfunction, molecular alteration in growing follicle are important components. COD has been diagnosed by animal behavioral changes, nymphomania, anestrus, repeat breeding, pelvic ligament relaxation, tails head elevation, determination of progesterone level in plasma and milk by using kits of progesterone assay, and to confirm ...

Xinjun Zhang1,2, Pengyong Wang1,2, Huimin Ju1,2, Yanhong Wang1,2, Yang Yang1,2* and Guoqiang Zhu1,2*

...text-align: justify;">Uropathogenic Escherichia coli (UPEC) is a common pathogen of urinary tract infection. To investigate its characteristics and explore the interaction between UPEC and human urinary bladder cancer T24 cells, 7 canine UPEC strains were isolated from dogs in Yangzhou, China. The adhesion-encoding genes (iha, fimH, papA, papC, papG allele I, papG allele I’, papG allele II, papG allele III, focA, focG,...
C.A. Angel-Sahagún1, J.E. Ortega Palomares1, A.A. Hernández-Rangel1, C. Cruz-Vázquez2, R. Montesinos-Matias3, M. Valencia-Posadas1 and A.M. Cruz-Avalos4*
...tudy was to evaluate the pathogenicity of Metarhizium anisopliae (Ma) and Beauveria bassiana (Bb) isolates, obtained from adult Ctenocephalides canis, the dog flea, under laboratory conditions. Nine, monosporic cultures were isolated. Each were pathogenic when exposed by immersion at a concentration of 1x108 conidia/ml, causing between 10-100% mycosis at ten days post-inoculation. Four isolates identified as Bb9, Bb6, Ma9 an...

Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6

Xiangli Dong1,2, Shilin Mikhail Borisovich2, Jiji Li1,*, Jianyu He1, Zeqin Fu1, Yingying Ye1, Julia N. Lukina3, Olga V. Apalikova4 and Jianshe Zhang1

...RC2 in defending against pathogenic bacteria challenge and the innate immune response in the large yellow croaker. These findings also provide a foundation for the preparation of a Vibrio vaccine.

...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...lecular studies on these pathogens to control and avoid their spread in turkey and other poultry flocks.

Keywords | Bacteria, Multiplex PCR, Sinusitis, Turkey  

...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...hicken from pH-sensitive pathogens. The purpose of this study was to define the bactericidal action of organic acids on Campylobacter jejuni, individually and in combination. Total 120 broiler chickens were randomly distributed in ten groups. The groups included negative and positive control, pure organic acid group and commercial organic acid formulation group. Excluding negative control group, all other groups were orally challenged with 0.1 ml of the 6-Log ...

Waqas Raza1*, Muhammad Usman Ghazanfar1, Muhammad Asif2, Ikram-ul-Haq3, Muhammad Zakria4 and Laith Khalil Tawfeeq Al-Ani5,6

...festans is a destructive pathogen in the tropical and the sub-tropical areas of the world. Identification of pathogens is very important to find successful disease control strategies. Here, we isolated and identified P. infestans, as well as, determined the morphological characteristics, on different media. The phenotypic characteristic of the isolates were observed as fluffy cottony growth with striated pattern. The average...

Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy* 

...tial role in controlling pathogens in health care, animal production, and food-related industries. The effectiveness of a disinfectant is mainly dependent on the active compound chosen, its concentration, and the cleanliness of the surfaces to which it is applied. The purpose of this study was to evaluate the effectiveness of some disinfectants (Prophyl 2000®, G7®, Pron-Tech®, Alkadox®, and Biodine®) at different concentrations and contact ...

Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3

...0, to identify bacterial pathogens of mastitis, and to determine the antimicrobial resistance. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical mastitis (SCM). Mastitic milk samples (n=541) were further used to investigate the prevalence of bacteria associated with mastitis and their antimicrobial susceptibilities by standard disc diffusion assay. The results showed that the p...

H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1 

...od to reduce the risk of pathogenic bacteria and reduce the use of antibiotics.

Keywords | Poultry farms, Hygiene, Enterobacteriaceae, Antimicrobial Resistance, Resistance gens. 

...

Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1* 

...>s 

...

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

...inhibition zones against pathogenic bacteria (E. coli and S. typhimurium). All isolates showed no hemolysis activity. Three isolates (A3, A4, and B1) are molecularly identified as Enterococcus faecalis which showed a potency as probiotic candidate. We conclude that Enterococcus faecalis is a potential probiotic that can be isolated from BSF larvae.

Keywords | 16S rRNA Gene; BSF Larvae; Chicken Manure; Lactic Acid Bacteria; Palm Kernel Meal 

...

Khalid Hussain1, Muhammad Younas1*, Niaz Hussain1, Abdul Ghaffar1, Muhammad Raheel2, Anees Akhtar3, Muhammad Irshad1, Munir Abbas1 and Farah Shabir4

...(11-20% DI) against wilt pathogen. However, five genotypes recorded moderately susceptible response (21-29% DI) and five genotypes showed susceptible response (30-50% DI) moreover; the remaining seven genotypes expressed highly susceptible response with maximum percent disease index (PDI) (>50%). Maximum and minimum disease incidence was recorded on CH-32/10 (7.26%) and D-15024 (69.61%) genotypes, respectively. It is concluded that chickpea resistant genoty...

Fawziah M. Albarakaty* 

...ic medically significant pathogens isolated from cows and poultry farms were assessed. Additionally, the phytochemical composition of the aqueous and ethanol peel extracts was examined. Results and conclusion: According to the findings, the plum (Prunus domestica L.) peel extracts have аlkaloids, flavonoids, tаnnins, and sаponin chemicals. It is, therefore, possible to conclude that plum (Prunus domestica L.) peel extracts have great antibacterial action op...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...IBK based on recombinant pathogenic factors have been evaluated as alternatives to whole cell vaccines. Pilin is one candidate antigen for M. bovoculi, and is a key pathogenic factor necessary for attachment of M. bovis to the surface of the eye epithelium. To evaluate pilA diversity amongst Kazakh isolates of M. bovoculi, the pilA gene sequence was determined for 35 isolates of M. bovoculi. Ten genotypes (gNA1-gNA10) were ...

Jie Yang1, Wenjun Zhou2, Qi Zhang2, Lei Chu2, Zhenshi Chen1, Xiajun Zhang2, Weidong Wu3*, Shaoru Zhang1* and Lihui Wang1*

...a gonorrhoeae is a human pathogen. Strain identification and effective antibiotic treatment are the main ways to prevent and control gonorrhea, but N. gonorrhoeae is now resistant to most antibiotics, and there is no vaccine available. The main purpose of this study was to understand the molecular characteristics of clinical isolates of N. gonorrhoeae and their resistance to azithromycin (AZM) in Danyang, China. Firstly, the clinical isolates of N. gonorrhoeae...

Yasemin Yıldırım Meşepınar and İlker Kepenekci*

...sect pests are the entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae. This study assessed the effects of EPNs (Nematoda: Steinernematidae) species [Steinernema feltiae (Balıkesir isolates) and Heterorhabditis bacteriophora (Çanakkale isolates) (Nematoda: Heterorhabditidae)] detected in our country (Turkey) against the larvae and pupae of P. operculella in greenhouse-pot experiments. S. feltiae was the most effecti...

Reza Aghnoum*, Hamid-Reza Sharifi, Masoud Ghodsi and Ahmad Zare Fizabadi

...A practices on non-plant pathogenic nematodes is not well understood. This study was carried out to determine the effect of CA on population density of fungivorous (Aphelenchus avenae) and free-living nematodes under four crop rotation patterns. The experiment was arranged as a split plot in randomized complete block design with three tillage systems (conventional tillage, minimum tillage and no-tillage) as the main plots and three level of crop residue retent...

Shahid Iqbal, Muhammad Aslam Khan, Muhammad Atiq*, Nasir Ahmed Rajput, Muhammad Usman, Ahmad Nawaz, Ghalib Ayaz Kachelo, Azeem Akram and Hadeed Ahmad

Man Wang1 and Fengmei Yang2*

...an important role in the pathogenesis of liver cancer, and the expression of TOP2A gene can be used as a prognostic indicator of liver cancer.

...

Muhammad Mudassar Shahzad1*, Hamna Rashid1, Syed Makhdoom Hussain2, Sana Bashir1, Fatima Khalid1 and Nisar Ahmad3

...ization, compete against pathogens, and provide resistance against diseases. Six experimental diets were formulated by utilizing an RSM-based meal supplemented with graded levels of probiotics such as 0, 1, 2, 3, 4, and 5 g/kg. For the experiment, triplicate tanks were utilized and in each tank 15 (avg. wt. = 6.76 g) fingerlings were stocked. Fish fingerlings were fed at the rate of 2 to 4% of live wet weight, the diet was given to the fingerlings twice a day ...

Hams M.A. Mohamed1*, Katreen K.G.2, M.W. Abd Al-Azeem1, Faysal A. Wasel2, Ahmed M. Abd-Eldayem3 

...ied as a major foodborne pathogen associated with high mortalities due to severe invasive listeriosis, meningitis and abortion in humans. With particular emphasis on Listeria monocytogenes’ capacity to form biofilms, antimicrobial resistance profile and virulence determinants, the purpose of this study is to discuss the presence of Listeria species in raw milk. In total, 150 samples of raw milk from vendors and nearby dairy farms in Sohag, Egypt, were co...

Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1 

.... 

...
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*

 

...xt-align: justify;">High pathogenicity island (HPI), a critical genomic element of pathogenic Yersiniabactin (Ybt) carries out synthesis, regulation, transportation as well as virulence. As a virulent determinant for the E. coli, the role of HPI in Saba pig was explored to provide some perspective about disease association. This is the first study in which 44 E. coli superior serotype strains were isolated and identified fro...

Amir Abdullah Khan and Muhammad Irfan*

...iculture caused by these pathogens. In the current study, 68 bacterial strains were isolated from citrus field soil and tested for antifungal activity using the streak plate method. Among them, one bacterium was found to be antagonist against the pathogen. This was identified as Bacillus subtilis 2i through 16SrRNA. The disease causing fungus was also isolated and identified as Schizophyllum sp. B2A through 18SrRNA.The strai...

Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1

...f different species. One pathogen of greatest importance that may be dispersed by birds are the coronaviruses (CoVs). Despite its relevance and complexity, studies on the detection and genetic characterization of these viruses are scarce, especially in South American wild birds. Thus, this paper aims to detect and discuss the diversity of these agents in birds at migratory stopping and wintering sites, along Brazilian Segment of the Atlantic Flyway. Therefore,...

H. M. Hassan† and S. A. Ibrahim

...ith two species of entomopathogenic nematodes, Steinernema carpocapsae and Heterorhabditis bacteriophora were tested against cotton leaf worm Spodoptera littoralis. All tested insecticides caused less mortality to the tested entomopathogenic nematodes. Magic smart significantly surpassed other insecticides causing the mortality of 11.6 and 13.8 % to the entomopathogenic nematodes S. carpoc...

A. Beyan1, A. Seid†2 and H. Shifa1

...t economically important pathogens of tomato. A study was initiated with the objectives of determining the interaction effect of M. javanica (MJ) and F. oxysporum f. sp. lycopersici (FOL) disease complex and evaluating the response of selected tomato genotypes against this disease complex under glasshouse condition. A glasshouse experiment was laid out in a factorial randomized design with 18 treatment combinations of three tomato genotypes (Assila, Cochoro, M...

A.H. Choshali1, S. Rezaee2, S. Jamali3, H. Reza3, Zamanizadeh4 and F. Rejali5

...nificant decrease in RKN pathogenicity factors (number of galls, eggs, egg sacs and J2) in both tolerant and susceptible cultivars. Inoculation of susceptible roots with AMF significantly reduced the nematode pathogenicity factors. Also the results indicated that the activity of both enzymes increased in the plant with AMF compared with the cucumber roots inoculated with M. incognita alone. Cucumber mycorrhizal roots showed ...

F. Shahina†, K. Nasira, K. Firoza and Y. I. Erum

..., soil, marine and entomopathogenic nematode species described and reported during last sixty nine years (1952-2019) from Pakistan. The information compiled in this paper came gradually from many and diverse sources. It is the product of the efforts of several authors who contributed to gather knowledge on the biological diversity through systematics and faunistic studies. Information published in different scientific journals, newsletters, reports, book chapt...
E. Yavuzaslanoglu1†, U. Gozel2, C. Gozel2 and M. Aydogdu3
...istribution of the entomopathogenic nematodes in apple growing areas in
Karaman province, Turkey and their efficacy on Galleria mellonella (Lepidoptera: Pyralidae). Soil samples were
collected from 130 apple orchards in April 2012 and 2013. Entomopathogenic nematodes were found in 25 samples
(19.23%). Entomopathogenic nematodes species isolated: Heterorh...

A. M. Rahoo1,2,†, T. Mukhtar3, S. R. Gowen2, B. Pembroke2 and M. A. Rahu4

...ditions.
...

A. M. Rahoo1,*, †, T. Mukhtar2, S. I. Abro3, S. R. Gowen1 and B. A. Bughio4

... chemicals, use of entomopathogenic nematodes
(EPNs) is among the viable alternatives. The successful management by EPNs is dependent on their establishment in
soils, infectivity, persistence and pathogenicity for longer periods. In the present study, emergence of Steinernema
feltiae infective juveniles (IJs) from infected Galleria mellonella cadavers was monitored under moist and dry

Ahmed S.M.H. EL Roby

...ne exotic isolated entomopathogenic
nematodes (EPNs) against sugarcane mealybug Saccharococcus sacchari under laboratory conditions (27 ± 2 °C,
RH 65 ± 10%). The EPNs used in the study are Steinernema sp. (strain: AT4), Steinernema sp. (strain: EMB),
Heterorhabditis bacteriophora (strain: EKB20), Heterorhabditis sp. (strain: EIK) and Steinernema glasri (strain:
New Jersey). The bioassays were ca...

I. Kepenekci1†, S. Toksöz2, T. Atay1 and I. Saruhan2

...div>Six species of entomopathogenic nematodes (EPNs) (Steinernema feltiae, S. carpocapsae and Heterorhabditis
bacteriophora) isolated from Turkey [S. feltiae (Aydin isolate), S. carpocapsae (Black sea isolate), H. bacteriophora
(Aydin isolate) and H. bacteriophora (Çanakkale isolate)] and from Kyrgyzstan [S. feltiae(KG3) and H.
bacteriophora (KG81)] were tested for the control of black timber bark beetle, Xylosandrus ...

I.K.A. Ibrahim1 and Z. A. Handoo2

...n rice plants.

...

F. Shahina† and G. Mehreen

...n this study seven entomopathogenic nematode Steinernema isolates were examined using genetic analysis by ITS
rDNA and 12S mtDNA. Phylogenetic analysis of these isolates was inferred by using four different methods i.e.,
Maximum Evolution (ME), Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbor Joining (NJ)
based on the two makers. Sequence composition and phylogenetic analyses of these isolates showed closeness wi...

R.R. Arain, R.N. Syed, A.Q. Rajput, M.A. Khanzada1, N.A. Rajput and A.M. Lodhi1

...Chitwood is the virulent pathogen of tomato
plants. The artificial inoculation of M. incognita developed a large number of galls and suppressed the tomato
plant growth significantly. Three different concentrations of neem extract, furadan and Trichoderma harzianum
were evaluated to control the M. incognita. All concentrations proved effective in reducing the disease
development in nematode inoculated tomato plant...

I. Kepenekci

...l of 82 species of entomopathogenic nematodes (EPNs) has been identified worldwide belonging to
Steinernema (65), Neosteinernema (1) and Heterorhabditis (16). This number is going up in parallel to new
investigations. Five species of Steinernema and three Heterorhabditis species were identified in Turkey. There are
only a few literature supports about detrimental effects of Turkey origin EPNs on maleficent groups having

F. Shahina†, K.A. Tabassum and M.A. Habib*

...genous
entomopathogenic nematodes (EPNs) isolates have positive results. Four EPN isolates viz., Steinernema
pakistanense, S. asiaticum, S. feltiae and Heterorhabditis indica were assessed for their infectivity against the
cotton bollworm complex in field. EPNs cultured on Galleria mellonella L. and stored in distilled water at 5 °C,
were kept at room temperature for 24 hrs before use. The number of bollworms...

Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk 

...out. To do this, several pathogenic bacteria and viruses from the Federation of European Laboratory Animal Science Associations (FELASA) were selected. The main methods of testing biological samples from laboratory mice and rats were realtime polymerase chain reaction and enzyme-linked immunosorbent assay. A high prevalence of some pathogens among conventional laboratory animals used by some academic institutes and universit...

Najam-ul-Sehar Afshan1*, Javeria Majeed1, Abdul Rehman Niazi1, Saima Khanum2, Maria Riaz1, Muhammad Fiaz2 and Shaila Anjum

...b, Pakistan, during phytopathogenic surveys in 2015–2020. The causal fungus was observed and identified on the basis of morpho-anatomical and molecular analyses. This is the first report of powdery mildew infection caused by Erysiphe necator var. necator from Punjab and Khyber Pakhtunkhwa provinces of Pakistan.

...

Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4

...t-align: justify;">Entomopathogenic nematodes (EPNs) are effective biocontrol agents against different insect pests. However, very little work has been done in Pakistan on the symbiotic bacteria associated with EPNs and their metabolites. In this laboratory study, two bacterial isolates i.e. Xenorhabdus spp. associated with EPN Steinernema glaseri (Steiner) (Steinernematidae) and Photorhabdus spp. associated with EPN Heterorhabditis bacteriophora (Poinar) (Het...

Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1  

...life may be shortened by pathogenic and spoilage bacteria, which are prone to contaminating food. This suggests that bio-preservatives may be used during the cheese-making process. So, this research was done to determine how well moringa oil (MO) and its nano emulsion (Nano-MO) work on some food poisoning bacteria inoculated into white cheese in vitro and in vivo. Prepared (Nano-MO) had the Z-average diameter of 76.04±51.13 nm and polydispersity index (...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...ucted to investigate the pathogeneicity of Newcastle Disease Virus (NDV) genotype VII.1.1 “NDV-CHICKEN-EGY-ALEX-NRC-2020” strain in combination with Infectious Bursal Disease Virus (IBDV) “IBDV/Egypt/Qalubia/17” and/or some commercial immunostimulants (Lector® and Orego®) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance...

Nachaat Sakr

...s of host resistance and pathogenic variation of FHB species need to be identified. Although barley and wheat are crucial crops in the dried Mediterranean area, there is insufficient information about their resistance to head blight infection and aggressiveness of Fusarium species. A 3-year (2019 to 2021) experiment was conducted under arid Mediterranean conditions to measure disease reactions, i.e., FHB incidence (DI, Type I resistance), FHB severity (DS, Typ...

Zarema Alimsultanova Magomedova1, Kheda Khalitovna Dadaeva1, Roza Said-Akhmedovna Zakhkieva1, Islam Khasanovich Shakhbiev1*, Boris Kazievich Laipanov2 

...culation of nematodiosis pathogens in the infrastructures of the foothill zone. In addition, it should be noted that these levels of environmental pollution by eggs of the genus Nematodirus are quite enough for the active implementation of the epizootic process of nematodiosis in animals in the North Caucasus region during the period (spring-summer-autumn).

...

Aqleem Abbas1, Mustansar Mubeen2, Yasir Iftikhar2, Qaiser Shakeel3*, Hafiz M. Imran Arshad4, Maria del Carmen Zuñiga Romano5 and Sarfaraz Hussain6

...om RSB. Recently various pathogenesis-related (PR) viz. PR-3, PR-5, PR-9, PR-10, PR-12, and PR-13 and WRKY genes have been discovered in rice crops which are engaged in plant defensive responses and responsible for various biological processes, including growth, development, stress, embryogenesis, and resistance to RSB disease. Conventional fungicides are now being replaced with eco-friendly fungicides and biological agents, including mycoviruses that can effe...

Khasan Khamidovich Shakhbiev1*, Saidkhamzat Saidmagomedovich Abumuslimov1, Larisa. Vakhaevna Taramova1, Islam Khasanovich Shakhbiev1, Svetlana Aleksandrovna Shemyakova2 

...ly had an etiotropic and pathogenetic effect against causative agents of echinococcosis and streptococcosis, of but also of synergism in the interaction 

...

Ghulam Sarwar Shah1,2, Maqsood Anwar Rustamani1, Rab Dino Khuhro1, Rehana Naz Syed1 and Abdul Mubeen Lodhi1

...t devastating soil-borne pathogens in the warmer climates of the world. It is more damaging in vegetables by causing seedling rots, root rot, pre- and post-emergence damping-off, cottony-leak, cottony blight, and stalk rot diseases. An advantageous combination of various factors makes the control of Pythium aphanidermatum difficult. However, synthetic fungicides provide quick and effective control. Therefore, we checked the sensitivity of different isolates of...

J. Salma and F. Shahina

...lecting strains of entomopathogenic nematodes for mass production on large scale to provide protection to crops against insects.

...

A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen

...b(255, 255, 255);">Entomopathogenic nematodes Steinernema carpocapsae

Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6

...re one of the dominantly pathogenic bacteria that involved in bovine respiratory infections. Its spreading problem is growing rapidly especially it could combine resistance with high-level of virulence traits that make it is more difficult to be treated. This study provides insight into the respiratory infections by Klebsiella spp. and its MDR profile in bovine cases, as well as the potential target genes that were involved in the links between virulence deter...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...propriate for monitoring pathogen in a very short time compared to laborious, non-specific and expensive protocols. ELISA, Nested PCR has been used for many years in different countries. Citrus orchards in Sargodha region were surveyed and leaf samples showing typical symptoms of citrus canker were collected. Infected section of leaf was taken for isolation of bacteria. Lesions were cut into parts and streak bacteria by the help of inoculation loop grown in nu...
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
...equired to determine the pathogenicity of the IBDV reassortant and development of new policies for IBDV intervention in the country.

...

Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*

...ginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes. Two hundred buffaloes were examined and sixty mastitis milk samples were collected from clinical cases from t...

Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1 

...quire to deepen into the pathogen’s response to different food environments and conditions. This study was conducted to quantify the growth behavior of S. aureus in Tryptone Soya Broth (TSB) using turbidity and plate count methods as well as monitoring and modelling its potential growth in chicken breast and thigh meats at room temperature (25 °C) and refrigeration temperature (4 °C). The calibration curve showed a linear relationship between the...

Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5

...eral medically important pathogens, evaluating the body length variation of Aedes aegypti larvae with the changing seasonal temperature is important. The study was conducted to observe the difference in body size and different body structures of Ae. aegypti larvae in two seasons, i.e., southwest monsoon (June through September) and retreating monsoon (October and November). The fourth instar larvae were collected from areas of district Lahore. The collected la...

Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3 

...e enhancer, reducing the pathogenic bacteria load, and boosting the immune response. PB may be a suitable alternative to AGP.  

...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...olation of the following pathogens from clinical and subclinical mastitis cases, respectively; E. coli (29.41% and 29.2%), S. aureus (19.12% and 25%), Str. agalactiae (16.2% and 16.6%), Str. dysgalactiae (14.7% and 20%), Str. uberis (7.3% and 2.5%), K. pneumoniae (4.4% and 3.3%), Str. fecalis (4.4% and 0%), Str. pyogenes (2.9% and 0%) and Pseudomonas aeruginosa (1.5% and 3.3%). Among the recovered E. coli serotypes, the serotype O111:K58 (B4) was the most prev...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
..., Pakistan. Isolation of pathogen from meat samples were performed with the use of International Organization for standardization based microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. coli. Among them, 14 (8.75%) isolates were tested positive for Stx genes (Stx-1 and Stx-2). The detection of pathotype of shiga toxin producing...

Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*

...ccus are one of the main pathogens causing bacterial disease such as mastitis and endometritis in dairy farming. The virulence factors produced by Streptococcus are related to the occurrence of inflammation. To investigate the correlation between antimicrobial resistance and virulence traits of bovine Streptococcal isolates. Induced resistance was conducted for Streptococcus pneumonia ATCC49619 and erythromycin-sensitive strains by gradually increasing the ant...

Meng Wang, Dongliang Zhou, Huixia Li, Chunqin Wu,Yan Zhao and Houqiang Luo*

...tems are involved in the pathogenesis role of bacteria, especially the formation of bacterial resistance. Therefore, the study of the bacterial secretion system is of great significance for antibiotic treatment of bacterial diseases. Most of the secretion systems identified so far are found in Gram-negative bacteria. There are a few secretion systems in Gram-positive bacteria, such as type VII secretion system, which is found in Mycobacterium tuberculosis. T5S...

Asmaa A. M. Abdel-Ghany1, Ahmed N. El Taweel2, Yassmin Moatasim2, Nagwa S. Ata1, Amany Adel3, Ayman Hany El-Deeb4, Ahmed Kandeil2, Mohamed A. Ali2*, Hussein Ali Hussein4* 

... />  

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Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2 

... which are the main food pathogens. In Ukraine, the distribution of emergency campylobacter has not been researched. This article describes conditions for active monitoring of Campylobacter spp. antimicrobial resistance, selection and research of 2,120 samples of cecal contents from cattle, pigs, and poultry. The samples were analyzed by a microbiological method for the detection of Campylobacter according to the scheme of accumulation, isolation, isolation an...

Bambang Hartoyo1*, Tri Rachmanto Prihambodo1,2, Wahyuningsih3, Sri Rahayu1, Fransisca Maria Suhartati1, Muhamad Bata1, Efka Aris Rimbawanto1

...Escherichia coli. Due to pathogen bacteria decrease, the histologic structures of digestive tract encounter improvement through minimizing damage of villus. In conclusion, lactobacillus supplementation in broilers increase performance due to improvement in the digestive tract and decrease in pathogenic bacteria with 5 x 10-7 cfu log-1 Lactobacillus population recommendation.
 
Keywords | Intestin...

Somia Eissa Sharawi

...he transmission of viral pathogens and the possible roles of some insects in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. In this study, we briefly review the possibility of biological or mechanical transmission of SARS-CoV-2 by insects in the environment.
 
Keywords | Insects, Biological transmission, Mechanical transmission, COVID-19, SARS-CoV-2
...

Sar Zamin Khan1*, Muhammad Waqas1 and Sagar M. Goyal2

...s variation, prevalence, pathogenesis and development of diagnostic tools are important for the formulation of effective prevention and control programs. This review deals with the global prevalence of IBV along with its pathogenesis, diagnosis, and control. In summary, we have discovered that measures to combat infectious bronchitis is not fully effective and the continued attention of the scientific community and funding a...

Sumbal Nazir1*, Farkhanda Manzoor2, Ishrat Perveen3, Anum Rana2, Irum Naureen1, Abad Ali Nadeem3 and Hafiza Najma Naeem2 

...l, protozoan, and fungal pathogens are attached on the honeybees worldwide. These pathogens affect the honeybee population by creating disease in it, which also effect the economy of country. It destroys the honey production and causes a subsequent reduction in the crop yield. In this present work, we isolated and screened various honeybee pathogens i.e., Nosema apis, Nosema cerus, Acute b...

Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1 

...ce and load of total and pathogenic bacteria in the different components of the mud crab hatchery. Selective agar media, a series of biochemical tests, and bacteria isolation kits were used for the isolation and identification of bacteria. The genus Vibrio, Aeromonas and Pseudomonas were identified in different components of the larval production line in the hatchery. V. mimicus, V. parahaemolyticus, V. harveyi and V. alginolyticus were the major species ident...

Lipigwe Lauya1*, Peace Nkiruka Okeke2, Chukwudi Chizorom Ibeh1, Bello Ozovehe Banimoh1 and Nanma Tongnan Cosmas1

...p of HIV-2 patients, the pathogenicity of the HIV-2 virus, and the clinical picture of the disease depending on the sex of individuals.

...

Abdulaziz A. Alaqil

...The infection with avian pathogenic Escherichia coli (EC) causes a great economic loss in the poultry industry. The current study aimed to investigate the effect of propolis (PR) inclusion in the diets of broiler on the immunomodulation and productive performance after challenge with EC infection. Four hundred broiler chickens (Cobb500, 1-d-old) were randomly distributed in equal numbers in 40 battery cages (10 birds per cage). Every ten cages were placed in a...

Aqleem Abbas1, Mustansar Mubeen2, Waqar Younus1, Qaiser Shakeel3, Yasir Iftikhar2*, Sonum Bashir2, Muhammad Ahmad Zeshan2 and Azhar Hussain1

...on, the evolution of new pathogen and pest strains causes catastrophes to GB’s cereal and horticultural Crops. Moreover, Traditional plant varieties that resist disease and pests have been supplanted with fragile but high-yielding cultivars. As a result, diseases like rust, smut, black scurf, early blight, late blight, potato leaf roll virus, potato virus Y, grey mold, mildew, crown gall disease, gummosis disease, and nematodes and pests such as insect p...

Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1

...management of infectious pathogens, many antimicrobial and antifungal substances were previously found from synthetic and natural sources. Numerous investigations have demonstrated that the compounds coumarins, flavonoids, phenolics, alkaloids, terpenoids, tannins, essential oils, lectin, polypeptides, and polyacetylenes are present in medicinal plants. These bioactive chemicals serve as a foundation for the creation of antibiotics that are utilized to cure in...

Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1 

...l material geared toward pathogens detection. The microscopic lesions found on most of the farms of the P group on day 35 indicated the ongoing of a regenerative process, contrarily to the farms of the D and G groups, in which the guts still underwent inflammation on day 35. Coccidiosis infection was heavily widespread on birds at day 35. Differences between the farm in D and P groups weren’t striking as the disease was detected on the intestinal portion...

Abdulaziz A. Alaqil*

...s persuaded by the avian pathogenic Escherichia coli threatens egg production and costs the poultry business significantly in lost revenue. This investigation was carried out to determine the beneficial effects of dietary Lactobacillus acidophilus (LA) on laying hens exposed to endotoxic shock induced by intravenously injection of lipopolysaccharides (LPS). A total of one hundred twenty, 40-week-old, laying hens from the Hy-line breed were allocated at random ...

Abdur Rauf1*, Farooq Jan1, Muhammad Qayash2, Zia-ul-Islam3, Rizwanullah1, Ikramullah Khan1, Muhammad Shuaib4, Muhammad Khalid5 and Samrin Gul6

...fungal species. The four pathogenic bacteria (Micrococcus luteus, E. coli, Staphylococcus aureus, Bacillus subtilis) and two fungi (Aspergillus flavus and Aspergillus niger) were examined. The above-mentioned various ethanolic crude extracts of H. eichwaldii revealed the highest potential (15.82±0.40), (18.12±0.56), and (17.41±0.51) against S. aureus, a moderate activity (13.31±0.64), (15.43±0.52), (14.35±0.64), (11.34...

Supawadee Piratae1*, Noraphat Khiewkham2, Nattawut Maungmungkun2, Chanakan Tippornwong2, Tossapol Seerintra2, Sirikanda Thanasuwan3, Luyen Thi Phung4 

...a platys are rickettsial pathogens that cause infections in the blood and have adverse health effects in animals, especially dogs and other canids. PCR targeting the 16S rRNA gene was performed to determine the prevalence of ehrlichiosis and anaplasmosis in 127 dogs collected from Rayong province, Thailand. To confirm the identification of these two pathogens, PCR was performed to detect the citrate synthase (gltA) gene foll...

Atef M. El-Sagheer1*, Aline F. Barros2, El-Sayed M. Abd El-Aal3, Mohamed M. Gad4, Doaa S. Mahmoud4 and Amr M. El-Marzoky3

...is mainly to clarify the pathogenic effects of both nematodes (Radopholus similis and Meloidogyne incognita, alone or in combination), as a histological modification in root tissues under natural conditions of banana cultivation. R. similis was observed in the cortical parenchyma as a feeding site, and for the first time, the feeding site extended to the vascular cylinder. In roots infested with M. incognita only, the laceration of the feeding site in the inne...

Alisher Safarov1*, Nasreen Nasreen4, Firuza Akramova5, Shukhrat Djabbarov1, Adolat Mirzaeva5, Javokhir Esonboev5, Djalaliddin Azimov5, Mourad Ben Said2,3 

...nown to transmit various pathogens that can cause numerous diseases in livestock, leading to detrimental effects such as skin damage, weight loss, anemia, reduced meat and milk production, and even mortality. Aim: This study aimed to investigate the species composition and prevalence of ectoparasites in domestic (dogs and cats) and wild predatory mammals (jackals, wolves, foxes, and reed cats) in Uzbekistan through regular parasite collections. Materials and M...

Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...asmiellus scandens being pathogenic was found mostly on the trunk of trees and is a semicircle convex-shaped basidiocarp. The species were studied for morphological characteristics. For molecular characterization, DNA extraction was performed from the samples using a kit , and PCR amplification  was done using ITS4 and ITS1F  primers designed from the conserved regions as described in the previous studies. The amplified PCR products were sequenced fr...

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

...tors that were linked to pathogenicity and the severity of infection. There findings suggest that poor sanitation and a reduction in goat health and nutrition were to blame for the high morbidity incidence of mycotic mastitis in Iraqi goats. 
 
Keywords | Goats, Dairy industry, Mycotic, Mastitis, C. albicans, virulence factors, Yeast’s virulence and biochemical indices
...

Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*

...and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistance. Therefore, targeting CSCs and their associated signaling pathways is crucial for effective treatment of GBM. In this study, we showed that alantolactone (ALT) hold the capacity to suppress stem-cell-like phenotype (SCLP) of glioblastoma cells. ALT inhibited the growth, reduced mammosphere formation and down-regulated the expression of CSCs markers such as CD1...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...10-27 mm) against tested pathogens (Escherichia coli, Klebsiella pneumoniae, Bacillus licheniformis, Pseudomonas aeruginosa and Bacillus subtilis). Secondary screening using ethyl acetate extracts also showed significant ZI in the range of 06 – 14.0 mm (P ≤ 0.05) against these pathogens thus confirming their bioactive potential. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) ...

Yongman Guo1,2, Jundong Tian1,2,3, Dong Yang1,2 and Jiqi Lu1,2,3*

...he first defense against pathogens, viruses and bacteria in animals. Fresh fecal samples of adult Taihangshan macaques (Macaca mulatta tcheliensis) were collected during mating and non-mating periods, respectively, to understand the influences of mating status on physiological condition of wild macaques, through variation in fecal cortisol and IgA levels. The results showed that. (1) mean concentration of fecal cortisol in mating period (AM: 1751.12 ± 1...

Gang Liu*, Na Xu, Zhizhong Gong and Jiahui Feng

... lakes. Nine potentially pathogenic species were identified across all samples, and the relative abundances of potential pathogens were significantly higher in the Shengjin Lake samples than in the Caizi Lake samples. These findings suggest that the gut fungi were highly sensitive to the diets of the geese at both lakes. Potential pathogenic species of A. erythropus should be further studi...

Mohammed Naji Odhah1,2, Faez Firdaus Abdullah Jesse1*, Bura Thlama Paul3,4, Bashiru Garba5, Zaid Mahmood1, Eric Lim Teik Chung6, Mohd Azmi Mohd Lila7  

...cessfully eradicated the pathogen. This investigation was undertaken to determine the antibody titre (IgM and IgG) in does challenged with C. pseudotuberculosis and its Mycolic acids (MAs) immunogen extract. About 12 healthy crossbred female Boer goats were assigned into three groups (A, B and C), each comprising of 4 goats. Group A (Negative control group) was inoculated intradermally with 2 ml of sterile phosphate-buffered saline (PBS-pH 7); Group B (Mycolic...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...ely. Next most prevalent pathogens were Salmonella spp. (78.3% & 58.3%), Shigella flexneri (61.6% & 53.3%), Pseudomonas flavescens (55% & 53.3%), Pseudomonas aeruginosa (40% & 35%), Staphylococcus aureus (33.3% & 25%), Streptococcus agalactiae (21.6% & 18.3%), Klebsiella pneumoniae (15% & 13.3%), Staphylococcus epidermidis (13.3% & 10%), Klebsiella oxytoca (10% & 8.3%), and Streptococcus pyogenes (6.6% & 1.6%) in marke...

Peter Amuguni Mbato*, Wanzila Usyu Mutinda, Noah Kipkoech Maritim, Nicholas Edwin Odongo 

... antibiotic residues and pathogens in meat suggest improved meat quality. The free-range broiler production system would be a better production system to improve the welfare and quality of the meat produced.
 

...

Tahir Rasool Qamar1, Sanaullah Iqbal1*, Muhammad Nasir1 and Habib-ur- Rehman2

... Enzymatic activities of pathogenic bacteria were lowest in this group and were significant (p≤0.05) when compared to group G2. Percentage of total aberrant crypt foci (ACF) inhibition was also higher (30.14%) in group G4 as compared to group G3 (18.55%). Elevated levels of short chain fatty acids were observed in chicory root powder groups particularly in G4 which having significant higher levels of acetate and butyrate compared to group G2. Group G4 also ...

Xu Yun-Ming1*, Sun Zhi-Yuan1, Yang Jian-Bo1, Bian Rong-Rong2, Ren Hong-Lin3, Zhong Si-Yuan1, Cai Yu-Hong1, Peng Jing1 and Bao Hua-Xia1

...dborne and opportunistic pathogen. It is widely distributed in soil, water and animal intestines. Its special spore structure easily causes foodborne infection to humans or animals. In order to monitor and prevent the spread of B. cereus, a rapid and sensitive loop-mediated isothermal amplification assay has been developed to detect it in uniformly items. According to the entFM gene of B. cereus, four primers were designed, and reaction components and conditio...

Muhammad Hussain1, Yasir Ali2*, Baber Iqbal1, Muhammad Azhar Iqbal1, Salman Ahmad3, Muhammad Zeeshan Majeed4, Hafiz Muhammad Aatif2, Muhammad Saeed1 and Muhammad Jawad Yousaf1

...nst leaf and stripe rust pathogens, are good sources for future breeding programmes.

...

Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1 and Iqra Nazir3

... interference, and human pathogenic potential. In this study, we have developed a Brucella abortus pUC19-K-UP-DN plasmid cassette for the construction of ΔpurD deleted mutant by deleting the purD gene which is responsible for de novo purine synthesis. For this, the pUC19-K-UP-DN plasmid cassette was initially constructed using pUC19, kanamycin, and purD upstream and downstream fragments. Then the cassette was electroporated into electro competent Brucell...

Asifa Majeed*, Amir Rashid, Palvasha Waheed, Asma Faryal, Aden Razaq and Ayesha Maryam

...y was designed to detect pathogenic genetic variants in APOA1 and ABCG1 genes associated with dyslipidemia in type 2 diabetic patients. A total of ninety subjects of both genders, aged between 30-70  years were randomly selected and further divided into a diabetic dyslipidemia group, a diabetic group and a healthy group. Genomic DNA was extracted from peripheral blood and subjected to the polymerase chain reaction using primers of the APOA1 gene and ABCG1...

Imran Nadeem1, Qurban Ali1, Muhammad Kamil Malik1*, Asad Aslam1, Imran Tariq2, Muhammad Bilal Bin Iqbal1, Muhammad Faheem Akhtar1, Sikander Ali3, Muhammad Jawad Saleem1, Muhammad Zubair3 and Aqsa Abbas1

...Capsule lures, one entomopathogenic fungus, “Beauveria bassiana, and one noval insecticide, e.g., Radiant 120 SC (Spintoram), were evaluated to check the percentage infestation reduction of Pectinophora gossypiella under field conditions under the RCBD design. The results showed that the effectiveness of the traps was excellent in the early days but declined as time went on. The results showed that Pb rope traps had maximum control over the pink boll wor...

Muhammad Nadeem1, Jamshaid Iqbal2, Tariq Mustafa3, Gul Rehman2, Muhammad Faisal Shahzad2, Muhammad Younas4,5*, Aftab Ahmad Khan6, Ameer Hamza2, Abdul Ghaffar1 and Muneer Abbas1

...agement technique. Entomopathogenic fungi (EPF) are currently used as biocontrol agents and are alternatives of synthetic insecticides in sustainable agriculture. The bio-efficacy of entomopathogenic fungi (EPF); Metarhizium anisopliae (PacerMA), Verticillium lecanii (Zimm) (Mealikil-VL), Isaria fumosorosea (Wise) and Beauveria bassiana (Bals) (Racer-BB) were investigated against the mung bean thrips, Megalurothrips distalis...

Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li

...e. To detect these three pathogens simultaneously, we designed specific primers based on the conserved gene sequences of the three pathogens available in GenBank. After optimization of the reaction conditions and system, we successfully established a novel multiplex PCR method for detection of the aforementioned three pathogens. The results show that the amplified fragments of interest wer...

MALIK ASIF HUSSAIN

...an important role in the pathogenesis of many
microorganisms. A number of animal and clinical studies have presented
importance and role of biofilms in various clinical conditions. This form of
growth enables them to grow in form of microbial communities, which
help them to grow and survive better. Resistance to antibiotics is usually
linked and described as a reason for better survival of microbes in...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

Nurul Fajrih1,2, Komang Gede Wiryawan1*, Sumiati1 and Suraya Kaffi Syahpura3

...um and L. rhamnosus) and pathogenic bacteria (E. coli and Salmonella). Bacterial growth Media using control media without sugar sources, MRS + glucose media, and MRS + banana corm extract (BCE) media. Based on the results of the study, BCE contains glucomannan by 33.59% with a yield of 14.15%, and in vitro test results show that BCE is only able to be fermented by the lactic acid bacteria but not by pathogens. In addition, B...

Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail

...in plant defense against pathogens attacks and different abiotic stresses. Current research deals with the study of cis-regulatory elements located in the promoter region of OsGLP12-3 gene of Nipponbare and newly sequenced Oryza sativa L. cultivar Dilrosh-97 using bioinformatics approach. The possible cis-acting regulatory elements were analyzed using PlantCARE and PlantPAN3.0. Phylogenetic tree was constructed using MEGA7 and docking of regulatory elements wi...

Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2

... to report the bacterial pathogens and their antibacterial resistance spectrum causing bovine respiratory tract infections in a large-scale cattle farm. Nasopharyngeal swabs were collected from animals (n=122) having clinical manifestations of respiratory tract diseases. Standard culture procedure followed by the Kirby-Bauer disc diffusion assay was applied to calculate the antimicrobial resistance profile of bacterial isolates. The survey results showed that ...

Frah R Kbyeh1*, Ahmed N. Abedsalih2 

... also have a role in the pathogenicity of the resistant Escherichia coli 0157: H7 strain. Resistance Escherichia coli 0157 H7 for antibiotics decreases the cure rate. Repurposing Food and Drug Administration (FDA) approved medications against the contributing factors is a novel method to overcome this scarcity. The analgesic medication diclofenac was reported to have anti-virulence action against resistance Escherichia coli O157 :H7. This study aimed to demons...

Kanda Yanuar Muhamad1,3, Trioso Purnawarman2, Chaerul Basri2* 

...ay potentially introduce pathogenic agents and facilitate their spread in Indonesia. The knowledge and awareness of importers and traders play a crucial role in mitigating the emergence of such potential risks. This research aimed to assess the level of knowledge among importers and sellers of reptiles concerning diseases that may be transmitted by these animals. A survey was conducted involving 113 employees working in reptile import and sales businesses all ...

Sidra Hafeez1, Tayyaba Sanaullah2, Hafsa Naeem3, Mah Noor Hassan4, Muttalib5, Farhana Kausar6, Muhammad Salman Hameed7*, Muhammad Anayat Ullah8, Abdul Samad9, Memoona Bashir10 and Sadaf Shabbir11

...’s postulates, the pathogenicity test was performed. CTAB method and Internal Transcribed Spacer region were applied to isolate and amplify the fungal genomic DNA respectively, by using universal primers ITS1/ITS4. The analysis of polygenetic of an amplified product revealed that black rot caused by D. bryoniae in pumpkin.

...

Nafissa Sahel1*, Fadela Chougrani2, Abderrahim Cheriguene3 and Zineb Hamani1

.... All samples tested for pathogenicity were negative for pathogenic germs.

...

Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3

...rium is an opportunistic pathogen of upper respiratory tracts of wild and domestic animals and birds as well as in livestock . The ompH gene was amplified with in-house designed primers, the amplified gene (1002 bp) was cloned into pET 40-b (+) vector and transformed into E. coli BL21 (DE3) competent cells using heat shock transformation method to get expression. The product size was confirmed by restriction digestion. The rOmpH protein was purified by immobil...

Dan Song1,2, Ming-Juan Ge3, Jie Li1,2, Yi Jiang1, Xiu-Mei Kong1, Jiao-Jiao Xu1, Xu Ji1,2, Rui-Xin Shi1 and Qin Zhao1,2*

...s. Male Kunming specific pathogen-free (SPF) mice were randomly divided into blank control, model (hypoxia), positive (propranolol 30 mg/kg + hypoxia), and three G. straminea ethanol extracts dose groups (10, 5 and 2.5 g/kg respectively + hypoxia), administered intragastrically once a day for 14 consecutive days. After that, multiple hypoxia experiments were conducted including soda lime normobaric hypoxia test, sodium nitrite poisoning test, isoproterenol poi...
Mahmoud M. Bayoumi
...against infectious viral pathogens. This technology has the potential to revolutionize vaccine development, offering a more efficient and cost-effective approach that can be tailored to specific viral strains. Moreover, mRNA-based vaccines offer several advantages over conventional or molecular-based vaccine types. The mRNA vaccine only encodes the target viral protein, with no infection hazard or even nucleic acid integration. Furthermore, mRNA vaccines can s...
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
...l methods of controlling pathogenic bacteria in which they have a high ability to self-reproduce, host specificity, and develop with their bacterial hosts. The most common pathogenic bacteria were isolated from fresh meat and identified using the VITEK II automated system. Phages specific to Escherichia coli, ...
Morcos Ibrahim Yanni1* and Eid Elsaid Abdelaziz2
...ne plays a role in viral pathogenicity, the detected variance in nucleotide and amino acid identities of the P gene in our local circulating isolates over the years confirmed the anticipation of distinction in the pathogenicity of such isolates. All vaccinal strains have a genetic distance from field Dakahleya, Beheira, and Alexandria isolates (ranging from 88.2% to 88.9% for the China human rabies vaccine AG)

Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1

...ntially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population on chicken breast cuts in the form of a phage cocktail. Out of 17 isolated bacteriophages, three bacteriophages (SEPL01, SEPL13, SEPL20) exhibited maximum lytic potential and broad lytic spectrum. Transmission electr...

Rana Mahmood Ahmad*, Orooba Mohammed Maeed  Ibrahim

...ytokines involved in the pathogenesis of chronic inflammation. Clove oil extract also increased levels of the anti-inflammatory cytokine IL-10, which is crucial in preventing tissue damage due to inflammation. These results led us to hypothesize that clove oil extract therapy speeds up the switch from an inflammatory to an anti-inflammatory response during the healing process. On the other hand, the positive control groups showed no improvement in the level of...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M. 
...-align: justify;">Highly pathogenic avian influenza (HPAI) caused by influenza A H5N1 virus, poses a significant threat to the poultry industry and humans in Egypt. Since it was first recognized in 2006, the disease has become enzootic in poultry throughout Egypt and still circulates in the poultry population, so the ability to rapidly recognize AlVs in biological specimens is critical for limiting further spread of the disease in poultry. Application of molec...

Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.

...r determination of their pathogenicity in specific pathogen free (SPF) 4-6 week old chicken. All the Al isolates proved to be HPAI viruses where the Intravenous Pathogenicity Index (IVPI) score for them were 2.1, 2.5, 2.3, 2.2 & 2.3 and their titers in ECE were 10.1, 9, 9.3, 9.3 and 10 Logo10 EID50/ ml for 2006, 2007, 2008, 2009 and 2010 isolates respectively.

...

El-Bramawyl , M . A. S. A. and El-Beshehy2, E. K. F.'

... is one of a devastating pathogen that reduces faba bean crop production in Ismailia. Resistance is limited and resistance-breaking isolates are becoming problematic. Effective BYMV disease control strategies will come from a better understanding of inheritance the resistance to BYMV disease in the crop considering. In this article we consider the determination role of the inheritance mode of the resistance to BYMV in FL, F2 and F3 faba bean populations. The F...

Sofyl *, A.R.; Soliman , A.M.; Mousal, A.A. and El-Dougdoug , Kh.A.

... cachexia isolate in the pathogenic domain (P) tend to influence the pathogenicity of the HSVd-EG. Finally, the genetic diversity and evaluation of entropy power for the Egyptian cifrus gummy bark agent and HSVd-citrus populations registered in GenBank, were viewed against the phylogenetic backgound of CVd-II variants including the noncachexia (CVd-IIa) and the causal agents of severe (CVd-IIb, CVd-IIc), more moderate (Ca903...

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

...lieved to control viroid pathogenicity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt

...

Karl Maramorosch

...yed the discovery of the pathogens of yellows-type diseases by forty years. In 1967 Japanese plant pathologists and entomologists announced the discovery of mycoplasma-resembling pathogens in diseased plants and insect vectors and the temporary recovery of diseased plants treated with tetracycline antibiotics. The recognition of mycoplasma-like structures was made accidentally by a veterinarian, Kaoru Koshimizu, but no credi...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K  and  Mona M.Aly 
 
...06 an epidemic of highly pathogenic avian influenza (HPAI) virus Of subtype H5N1 affected all commercial poultry sectors as well as rural and backyard level in Egypt. This outbreak also was extended to include the zoo birds In Giza zoo. This study records the isolation and characterization of H5Nl virus from different species of the zoo birds and also studies the immune states of the vaccinated birds after application of H5Nl vaccine for the first time one mon...

Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty

...-align: justify;">Highly pathogenic avian influenza (Al) H5N1 strains have been isolated during February 2006 from infected chicken located at El-Qanater El-Khiria center, Qaluobia, Eypt. These isolates were molecularly characterized and compared with those H5Nl strains isolated during January 2007 from the same locality. The strains isolated from infected chicken during 2006 were closely related to A/ty/Turkey/l/05 strain which had been isolated from tur...

Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman

...en prepared from the low pathogenic avian influenza (LPAI) virus A/Turky/CA/209092/02, H5N2 for the first time in Egypt. Virus strain from which the vaccine seed virus has been prepared was derived from the USDA, Ames Iowa, USA. The specific characters of this strain were studied; It was found that the best virus dilution of Al virus for egg inoculation was 10-3 to 10-4, the peak virus titer was obtained 72 hours post inoculation, the predilection sites of the...

Ahmed Abd El Samie Hassan Ali

...s (BVDV) is a world wide pathogen infects a wide variety of ruminants. A random 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits were observed for 25 day, slaughtered then tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for second inoculation. A another 16 BVDV seronegative N...

A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1

... 6/9 BVDVs that were cytopathogenic. Direct sequencing of the RT-PCR amplicons revealed an extreme nucleotide sequence homology (≥ 98%) among all tested Egyptian isolates versus the local vaccine strain. sequence alignments showed variable identity (74% - 93%) of the Egyptian vaccinal strain versus reference laboratory and vaccinal strains of BVDV. Clustering and phylogenetic analyses suggested that these Egyptian vaccinal and field BVDVs are low-Kinetic va...

H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1

...ic effect (CPE). The cytopathogenicity of the isolates were different when these viruses were inoculated on BVDV-free and-infected cell lines. To study the discrepancies on the growth behavior of the local isolates, the viruses subjected to further passages on MDBK cells infected with non-cytopathic (NCP) strain of BVDV which was homologues (pair) to their genotype (type I). After four passages on cell culture. The local isolates demonstrated enhancement in th...

H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3

...s-based AC-ELISA and the pathogenicity of some representative infectious bursal disease virus (IBDV) field isolates were studied. Of the selected 22 IBDV -positive bursal samples, 59.1% (13/22) were typed as classic IBDVs and 40.9% (9/22) were of variant IBDVs. The majority of IBDV variant antigens detected (89% Of IBDV variants) were related to IBDV Del/E variant strain and one sample (11% of IBDV variants) was related to RS593 strain. The

Ahmed Abd El-Samie H. Ali

...us (BVDV) is a worldwide pathogen infecting cattle. Eleven Holstein calves between 6-8 months of age that were seronegative to BVDV were divided into three groups (n=4 for NY-1, n=4 for 890 and n=3 control). Fibroblast cells were prepared from calves' skin biopsies by trypsin digestion. Using immunofluorescence. the percentages of infected cells and MHC antigens expression were evaluated. fibroblast and peripheral blood mononuclear (PBMC) cells were in vitro i...

N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.

...d injected into specific pathogen free (SPF) laying hens, from which their eggs were collected, the immunoglobulin-Y (IgY) was precipitated from the egg yolk of immunized hens by 33% saturated ammonium sulphate solution. The anti-rabbit immunoglobulin-Y (IgY) was purified by using affinity chromatography. The obtained globulins were divided into 2 parts. The 1st one was conjugated with peroxidase and the 2nd part was conjugated with fluorescein isothiocyanate....

Rizqan*, Hilda Susanty, Linda Suhartati, Azhar, Arief, Elly Roza 

...eus and Escherichia coli pathogens. Eco-enzyme was prepared from citrus peel waste, resulting in a solution with a typical fresh sour aroma. The pH of the eco-enzyme ranged from 3.7 to 4.5. Conducting the agar diffusion test revealed a dose-dependent inhibitory effect of the citrus fruit peel eco-enzyme against both Staphylococcus aureus and Escherichia coli. These findings underscore the antibacterial potential of the citrus fruit peel eco-enzyme solution and...

Tarek Refaay, E.A. Elshafiee, Hayam A. Mansour and Maha A. Sabry*

 

...lusion, the detection of pathogenic MDR E. coli O157:H7 in food samples, food handlers and food equipment in some touristic cities in Egypt poses a serious risk to public health. Therefore, it is recommended to focus on identifying practices which increase the risk of food contamination, and on implementing measures to improve the sanitary conditions in the food outlets in touristic cities.

...

Sajjad Khan*, Naila Chand, Abdul Hafeez and Nazir Ahmad

...omorphology and selected pathogenic bacteria in broiler chickens. Day-old 200 Ross male chicks were allotted to five groups each subdivided in four replicates with ten birds per replicate. Similarly, five different types of feed i.e., diet A as control while B, C, D and E having 1.5% GA, 30mg/kg BS, 1.5% GA+30mg BS and 0.75% GA+15mg/Kg BS respectively, were offered during 42 days of experimental trial. Statistical analysis was performed by SPSS following CRD d...

Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4 

...n dealing with foodborne pathogens. The number of Listeria monocytogenes outbreaks caused by eating food is increasing worldwide, and people are increasingly looking for natural ways to stop the development of L. monocytogenes. In this context, propolis, a natural substance produced by bees, looks like an attractive option owing to its antibacterial action against many pathogens in food. The aim of this study is to investiga...

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

...ne of the most important pathogens in the poultry industry and often causes diseases of a chronic and persistent nature. However, there is limited data available on Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway genes expression in chicken erythrocytes infected with Mycoplasma synoviae (M. synoviae). Therefore, the aim of the current in-vitro study was to determine the interaction between chicken erythrocytes and...

Muhammad Akram1, Anirban Mandal2*, Mehwish Iqbal3, Arindam Mukherjee4, Ritika Bandyopadhyay5, Surendar Rangasamy6, Rida Zainab1, Muhammad Talha Khalil1, Pragnesh Parmar7 and Umme Laila1

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...: justify;">Vector-borne pathogens have become a major problem since different species of cattle, e.g. indigenous, crossbred dairy, and crossbred beef, are raised together on most farms. Anaplasma spp. are obligate intracellular rickettsial vector-borne pathogens that impact on livestock farmers with major economic constraints and eventually threaten human health in cases of zoonotic species involvement. Therefore, our study...

Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil

...s checked. Intracerebral pathogenicity index (ICPI) of the attenuated strain was 0.2, the mean death time (MDT) was 120 hours and infectvity titer was 7 log10 EID50/ml compared to 1.97 ICPI, 36 hours MDT, and 9.5 log10 EID50/ml of the original strain. The attenuated AOAV-1 was protective in vaccinated chicks by means of HI antibody response and protection from heterologus virulent challenge while showing little to no residual pathogen<...

Aalaa S. Saad1*, Amira A. Elokle2, Dalia Y. Youssef2, Neven M. Omara2, Samr Kassem3

...for inhibiting foodborne pathogens. This study aimed to evaluate the antibacterial activity of synthesized Chitosan nanoparticles (CS-NPs) and CS-LZ-NPs against E. coli O157 in chilled chicken fillets and relative eaeA (Intimin), stx2 (Shiga toxin) expression. The average particle size was 250 nm and 281.4 nm for CS-NPs and CS-LZ- NPs and the PDI (polydispersity index) was 0.09 and 0.58, respectively. TEM imaging showed uniformity and ranging in size from 13.1...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
...as fluorescens are major pathogenic bacteria in freshwater aquaculture and cause huge economic losses. The outer membrane lipoprotein Slp of A. hydrophila has potential applications in fish vaccines. Slp bioinformatics analysis showed that anti-Slp serum might provide cross-protection to resist bacterial infection in fish. Slp was obtained by molecular cloning, expression and purification, and the expression conditions were optimized. In mice immunized with Sl...

Hongsen Xu*, Xiaoni Wang, Tie Tian, Changyu Zhao, Denghang Yu and Jun Liu

...this study confirmed the pathogen of whitish muscle disease in farmed P. clarkii, and preliminarily investigated the biological characteristic, pathogenicity and drug sensitivity of this bacterium. The results of this study will provide scientific guidance for the effective prevention and control of muscle turbidity of P. clarkii.

...

Waseem Abbas1, Dildar Hussain Kalhoro1*, Hasina Baloch1,  Muhammad Abubakar3, Muhammad Saleem Kalhoro1, Neluma Wali2, Mazhar Hussain Mangi1, Azhar Ali Laghari4, Shahid Hussain Abro1, Rani Wagan1 and Mehkar Hussain5

...evalence of an important pathogen in yak, zo and cow of Pakistan.

...

Kalsoom Abdulrazaq1*, Bisma Arif1, Rimsha Mehboob2, Asma Mehboob3 

...d other parasites. These pathogens have the ability to infect both domesticated and wild animals. Zoonoses involve interactions among a minimum of three species: an infectious agent and two host organisms, which can either be humans or animals. They can spread directly through contact with diseased animals, such as rabies, and through bites, or indirectly through exposure to contaminated surroundings and contaminated food. Vectors, such as mosquitoes or ticks,...

Kashif Abdaal, Aneeza Batool, Muhammad Tariq Navid*, Saeed Ahmed, Asma Saleem Qazi, Waseem Safdar, Haidar Ali, Mobeen Ur Rashid, Somia Rafaqat 

...ment(s) of an infectious pathogen(s), nanoparticle-based and plant-based delivery mechanisms have proven as more targeted and adaptable approaches to boost immune responses. Hence, it proves the vaccines as a big breakthrough in preventive and therapeutic medicine over 200 years and named “Future Medicine”. To assess vaccine safety and efficacy, scientists are now using various model animals like mice, ferrets, pigs, and nonhuman primates before us...

SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1

...) to check the growth of pathogenic fungi compared to controls. Aureobacterium liquifaciens (27.66%), Acinetobacter sp. (25.66%), Bordetella pertussis (26.63%) also showed equal potential for inhibition. In contrast remaining isolates Enterobacter cloacae (19.33%), Azomonas agilis (17%) and Kurthia sp. (19%) were less efficient as compared to the others. Bacterial endophytes are colonized inside plants and have antagonistic potential for fungal

SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1

...dwide. A large number of pathogens can colonize urinary tract due to enriched chemical composition of urine making it favorable for microbial growth. Current study was designed to investigate bacterial associated UTI and antimicrobial susceptibility pattern was determined by Kirby Bauer’s disc diffusion method. Samples (n=85) were analyzed to determine the bacterial strains involved in infectious cases and relationship with age, gender and social status ...

ZUBIA RASHID1, ZULFIQAR ALI MIRANI2, SITWAT ZEHRA1, SYED MUDDASSAR HUSSAIN GILANI3, ARIF ALI CHISHTI1, ABID AZHAR1 & SADDIA GALANI1*

...egies for drug-resistant pathogens is desired. Here, Phytogenic Feed Additives (PFA) were administered in chicken diet to decipher their impact on animal performance and gut microbiota composition. One-hundred-fifty-day-old chicks were randomly divided into three treatment groups with five replicates/group as (i) Control diets (CON); (ii) Control diets + 0.01% w/v enramycin (antibiotic growth promoter, AB); (iii) Control diets + 3% w/v blend of garlic, pepperm...

*AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR & ZOIA ARSHAD AWAN

... significantly decreased pathogen biomass by 6-96% and three Trichoderma species also caused significant inhibition in pathogen growth variably between 30-100%. In potted soil, application of separate or combined application of myco- and phyto-fungicides proved highly efficient in managing collar rot disease. However, combined effect of T. harzianum or T. viride + leaves biomass of R. sativus found more promising in managing...

Anisa Mushtaq1, Murtaz ul Hasan1*, Asim Shamim2, Muhammad Ali Abdullah Shah1, Muhmamad Arif Zafar3, Abdul Asim Farooq4, Aayesha Riaz1, Muhammad Kamran1 and Saif ur Rehman

...or of different types of pathogens like viruses, bacteria, rickettsia and protozoa and cause mortality in humans and animals. This study was focused on morphological and molecular identification of ixodid ticks, using morphological keys and an internal transcribed spacer (ITS-2) Deoxyribonucleic acid (DNA). Moreover, the presence of Babesia and Theileria species was also investigated in ticks using 18S rRNA gene. Identification of ticks collected from 384 catt...

Nadir Ali Birmani1*, Ramesh1, Shakeel Ahmed Memon2, Raheela Noor Memon1, Badar Alam Samejo3 and Mehtab Ali Mahar3

Habibeh Jabbari

...only differences between pathogenicity populations.

...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4
...hoid has revolved into a pathogenic hazard to the poultry industry and extensive use of antibiotic is leading to the induction of antimicrobial resistance in microorganism. Main aim of this study was to find out substitute of antibiotic against Fowl Typhoid in broiler in terms of immunological, serum biochemistry and lipid profile parameters. Broiler chicks (Age = 1 d and n = 90) were kept under uniform management conditions. Chicks were divided randomly into ...

Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1

...rted drawbacks including pathogenic potential for humans and excreted in body fluids of vaccinated animals. In the present study, we constructed a more attenuated B. abortus ΔpurD mutant by deleting the purD gene from the RB51 strain through site-directed mutagenesis. For virulence attenuation comparison with the parent RB51 strain, the constructed mutant was evaluated for attenuation estimation and clearance in BALB/c mice. The B. abortus ΔpurD mu...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...as done by isolating the pathogen during microbiological cultivation. Therefore, the high correlation (r=0.76, P<0.05) between the results of express testing and the detection of the pathogen by bacteriological cultivation, as the main diagnostic method of emphysematous carbuncle, allows recommending its use if it is necessary to diagnose the disease in pasture conditions quickly. 
 
Keyw...
Yanis Cruz-Quintana1*, Ana María Santana-Piñeros1, Byron Manuel Reyes-Mero1, Leonela Griselda Muñoz-Chumo1 and Lenin Cáceres-Farías1,2 
...nsidered one of the main pathogens affecting cultured fishes, mainly in small organisms or early ages. However, its incidence and effects on Amazon traditional aquaculture species such as Piaractus brachypomus has been little studied. The objective of the present investigation was to establish the presence of Trichodina heterodentata, its parameters of infection, and associated damages in three aquaculture farms of P. brachypomus located i...

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...n environmental mastitis pathogen and a significant contributor to clinical mastitis cases in dairy cows. Due to environmental nature of this pathogen, conventional mastitis control measures often prove ineffective. Hence, the endeavor to combat E. coli mastitis through vaccination appears feasible and economically prudent. This study was aimed at preparation and evaluation of mastitis vaccines prepared by using local E. col...
Danish Riaz1,2, Syed Makhdoom Hussain2*, Majid Hussain3, Muhammad Zubair-ul-Hassan Arsalan4 and Eman Naeem2
...ive function against gut pathogenic microorganisms. The purpose of this study was to examine the effects of supplementing Catla catla fingerlings fed a diet including sunflower meal (SFM) with probiotics (Protexin®) on growth performance, nutritional digestibility, and hematological indicators. Six test diets and one control diet (0 g/kg) were formulated before the beginning of the experiment, with varying amounts of probiotics (0, 0.5, 1, 1.5, 2, 2.5 and ...

Muhammad Rafiq,1 Amna Shoaib,1 Arshad Javaid1

... sclerotial forming phytopathogens. In the present study, methanolic root extract of
Sonchus asper was assessed for its antifungal activity against R. solani. The methanolic
extract was prepared by soaking dried root biomass in methanol and analyzed by GC-MS.
Antifungal potential of the extract was detected in test tubes filled with malt extract broth.
The results revealed fungal biomass was significantly reduced...

Arshad Javaid, Amna Ali, Iqra Haider Khan and Amna Shoaib

...um rolfsii, a soil-borne pathogen of over 500 plant species, causes collar rot
disease in chickpea plant and reduces its survival rate, growth and yield. This study was
undertaken to assess the benefits of soil amendment with a weed Chenopodium album L.,
on growth, yield and physiology of chickpea var. Bakhar-2011, against S. rolfsii. For this
purpose, soil was sterilized by fumigation with formaline and then ino...

Kiran Rani1*, R. Aliya1, Barkat Ali Solangi2, M. Kashif Pervez2, Nasrullah Akhtar3 and Farman Ahmed4*

...vestigated against human pathogens such as Escherichia coli, Staphylococcus aureus and Candida albicans and were compared with commercial antibacterial and antifungal drugs. It was found at the optimized dyeing condition has 1 percent formic acid as fixing agent with 120 min dyeing period. At this condition, the dye molecule shows 70 percent exhaustion and 59 percent fixation along with acceptable fastness and antimicrobial activities Furthermore, the dyed fab...

Shazia Shafique1, Sobiya Shafique1 and Sonia Sahar1

...ainst eggplant leaf spot pathogens i.e., Exserohilum rostratum, Cladosporium cladosporioides and Curvularia clavata. For this, 10 concentrations of methanolic extract of plant (0.5% to 5%) were employed against the pathogens. Data analysis depicted that development of all the pathogens was greatly inhibited by all the concentrations while 5% concentration found to be the utmost operative i...
Hasina Baloch1, Rahmatullah Rind2, Dildar Hussain Kalhoro1*, Shahid Hussain Abro1, Hubdar Ali Kolachi3, Saqib Kakar1, Muhammad Ibrahim3, Fayaz Ahned1, Hasnain Ali1 and Muhammad Mahesar1
...s, 57 (17.27%) contained pathogens. The correlation between SCCml-1 and colony forming unit (CFU) ml-1 of bacterial population was analysed. The mean SCC was counted as 82x105 cells ml-1in clinical and 50x105 cells ml-1 in subclinical mastitis. Staphylococcus aureus was recorded as the most dominant species. The mean values of 93x105 CFU ml-1 were counted for clinical mastitis while 51x105 CFU ml-1 was measured for subclinical mastitis. A clear difference betw...

Nabeel Akhtar1, Owais Iqbal1,2*, Imtiaz Ahmed Nizamani1, Rehana Naz Syed1 and Abdul Mubeen Lodhi1

...tify;">Soil-borne fungal pathogens are considered the most critical pathogens responsible for the enormous losses in chili crops. The diseases caused by them are often challenging to control due to different incidence level and limited knowledge of their epidemiological features. Advanced accessibility and a simple application process for chemical control systems have made it practical and widely accepted. In the present stu...

Amir Afzal1*, Sairah Syed1, Hafiz Husnain Nawaz2, Ruqeah Mustafa1, Marjan Aziz1, Madeeha Khan1, Azra Khan3, Uzma Javed1, Attiq Ur Rehman4, Rubab Altaf 5 and Qamar Shakil6

...tanding the lifecycle of pathogens is vital in formulating effective management strategies for plant diseases. Pst has a complex life cycle that entails both sexual and asexual reproduction, facilitating the rapid evolution of new strains. The evolution of new races is primarily driven by the selection pressure imposed by the deployment of resistance genes in wheat cultivars. Work documented by prominent researchers conducted in diverse regions of the planet h...
Irshad Ur Rehman,SAEED ULLAH KHATTAK,Muhammad Kamran,Jamshaid Ahmad,Abid Ali Khan,Ghosia Lutfullah

Safdar Abbas1, Jawaria Ali Khan1*, Syed Saleem Ahmed1 and Aftab Ahmed Anjum2

... (BCoV) is a major viral pathogen linked to respiratory and enteric problems in newborn calves. The goal of this study is to look into the molecular occurrence, haemato-biochemical changes, and risk factors associated with the occurrence of BCoV infection in cattle calves at different dairy farms and small households in the district of Jhelum, Pakistan. From July 2020 to June 2021, 200 faecal samples were collected from newborn cattle calves exhibiting symptom...

Roshan Ara1, Muhammad Ali Khanzada1, Amir Khan Korai1*, Abdul Mubeen Lodhi, Anam Mehwish Khanzada1, Khalid Hussain Qureshi1, Shakal Khan Korai2*

A. S. M. Helal Siddiqui1, Abul Khair2, M. Masudur Rahman3 and S.M. Mosfeka Hasnin4
...and confirmed two fungal pathogens for causal organism namely Phaenerochaete subglobosa and Schizophyllum commune and locally identified Ganoderma appalanatum....
Muhammad Afzal, Aqeela Mobeen Akhter and Masood Amjad
...nfect them from any pest/pathogen infestation. Auxins used were Indole Acetic Acid (IAA), Indole Butyric Acid (IBA), Naphthalene Acetic Acid (NAA) and other commercial rooting hormone (powder form) with different concentrations and combinations. It has been researched out that true to type Shisham tube plants can be produced vegetatively through the application of rooting hormones, which are fit for field planting with in a period of 4 months. NAA with a conce...
Mohammad Ayaz
...reak of forest pests and pathogens, which some times may attain the status of an epidemic, mainly under the influences of climatic conditions. Such catastrophes are controlled partially by human efforts and partially due to normalization of climatic regimes. The losses remain mostly limited to a tree species or an area and do not spread to other parts due to physical, climatic and species barriers. Besides others, the main physical cause of forest injuries in ...
Muhammad Afzal, Muhammad Mushtaque, Aqeela Mubeen Akhtar, Nighat Chughtai and Shaheena Ramzan
...o produced are devoid of pathogens. Plantlets of Ipil Ipil (Leucaena leucocephala) were obtained through multiplication of ex-plants produced from aseptically raised germinates of the species. In the basal media (MS + 5 micro molar BAP), ex-plants started differentiating shoot buds at high frequency. These aseptically developed plantlets were shifted to earthen pots for rooting without applying any root-promoting hormone. These plantlets showed a good g...
Parvez Khawaja, I.A. Hafiz and M. I. Chaudhry
...fer to the environment a pathogenic fungus, Beauveria bassiana (Bals.) Vuill. Was tried against the larval stages of the pest. The fungus displayed its pathogenecity by killing 72.5-97.5% larvae in various treatments as against 50% natural mortality in check treatment. The method can safely be used against the pest on larges scale....
Khial Badshah and Zakaullah
...d fall prematuiely. The pathogen was found to invade the plants through injured roots. Plant debris, infected young transplanting mateiial and seeds served as source of infection. The pathogen proved specific to Ocimum spp.

Vergouskii (1958) studied some peculiarities in the development of Fusariosis in basil. The high temperature at which basil seedlings were forced was found mainly responsible for the e...

Zakaullah and Shahida Perveen
...revalent and destructive pathogen on stored bulbs....
Zakaullah and Khial Badshah
...ysmptoms caused by the pathogen were similar to those of P. aphanidermatum and P. myriotylum, occurring at lower elevations, known as damaging agents to the crop. Besides, 50 to 90% of stored seed rhizomes are destroyed by the destructive pathogens if untreated....
Zakaullah
...ion courts for the decay pathogens....
M. A. Quraishi, A. Khalique, Shahida Perveen and Perveen Akhtar
...d is an important forest pathogen. It is most widely distributed dwarf mistletoe in the northern hemisphere, and among hosts it mainly infects the species of Juniperus (Hawksworth 1972) During British India, Brandis (1906) and Parker (1924) reported it on juniper but its pathological significance was brought up recently by Jamal and Beg (1974) while conducting a survey of the diseases of forest trees. According to Zaka (1977) about 36 percent of the trees in S...
Zaka-Ullah
...ract:
14 fungal pathogens were recorded on Willows growing in different areas of West Pakistan. Melampsora epitea (Kze. and Schm). Thuem. appeared in an epiphytotic form on nursery plants. Absence of large scale and compact plantations of Willows seemed to be a limiting factor in the appearance of few other pathogens in epiphytotic forms. No bacterial or virus disease was observed....

Pavana Jyothi Vanjavaka1, Mouradam Veerasami2, Mohana Subramanian Bhaskaran2*, Vijay A.K.B. Gundi1*

...CPV) is a contagious and pathogenic virus in puppies and dogs. It was first reported in 1978; however, it was later replaced by three antigenic variants at different periodic intervals that now circulate globally with random strains. This particular study aimed to determine the genetic changes in the VP2 gene, which are responsible for creating different antigenic variants, and to understand their impact on pathogenicity. In...

Ahmad Shandookh Hameed1, Ali Abd Kadhum2 and Murtada Wafi Beden1

...he most common secondary pathogens ,their antibiotic resistant profile and mortality rate of SARS-CoV-2 patients. A total of 41 patients with ventilator associated pneumonia of whom 23 were confirmed positive. for SARS-CoV-2 (infectious group) and 18 were tested negative for SARS-CoV-2 (non-infectious), were recruited in this study during the period from February to September 2022 at Al-Hussein. Teaching Hospital in Thi-Qar province, Iraq. Endotracheal aspirat...
Javairia Mehboob1, Syeda Hafsa Ali1*, Fahima Ashraf Kasi1, Syeda Ayesha Ali2, Safa
Farooqi3, Muneeza Arbab4,
...ic nanoparticles against pathogenic
bacterial strains (Staphylococcus aureus, and Escherichia coli) and Plant pathogenic fungal
strains (Aspergillus flavus and Aspergillus niger). Our results confirmed the formation of
AgNPs with size <100 nm. Antibacterial activity of lavender mediated AgNP was highly
significant, followed by artichoke mediated AgNP and Alkanet AgNP. Howeve...

Zara Naeem1, Khajista Jabeen1*, Muhammad Khalid Saeed2, Sumera Iqbal1

...L.) Pers. against target pathogenic mycotoxin producing fungal species (Trichoderma viride Pers., Trichoderma harzianum Rifai. and Cladosporium cladosporioides (Fresen.) G.A. de Vries. For this purpose, different concentrations viz. 2%, 4%, 6%, 8%, and 10% of methanolic leaf extract were prepared and tested for their antifungal potential in a completely randomized design. All the applied concentrations of S. halepense leaf extract inhibited the growth of all t...

Muhammad Nadeem1, Jamshaid Iqbal2, Muneer Abbas1*, Niaz Hussain1, Muhammad Tariq Javeed1, Abdul Ghaffar1, Muhammad Irshad1, Muhammad Aslam1, Gul Rehman2 and Shahar Yar Ahsan1

...ernel extract, the entomopathogenic fungus Beauveria bassiana and synthetic insecticides (acephate and chlorfenapyr), were tested individually and in various combinations for managing thrips (Megalurothrips distalis). A mungbean genotype, 13TM-04, known for its comparative resistance, was chosen for these trials. The treatments, whether applied alone or in various combinations, showed significant differences in efficacy when compared with each other. A combina...

Mohammed Mijbas Mohammed Alomari1, Nawar Jasim Alsalih2*, Samir Sabaa Raheem2, Mohenned Alsaadawi2, Ali Mosa Rashid Al-Yasari3

...f the epizootic process, pathogenesis, and treatment of dogs with parvovirus enteritis complicated by associations of coccidia remain poorly understood. The aim of this work was to develop and evaluate the effectiveness of a method for treating dogs with parvovirus enteritis, complicated by associations of coccidia, with a severe course of the disease. The study was carried on in Veterinary Medicine Hospital of Al-Muthanna Province, south of Iraq. Puppies with...

Muhammad Usman1, Hussain Abbas1, Riffat Maqsood1, Muhammad Awais Nadeem1, Abdul Hanan Shazal1, Ali Usman1, Amna Hameed2, Haram Shahid3, Zulqarnain Haider3, Muhammad Zain1, Muhammad Suleman1 and Muhammad Wasif Gulzar1*

Muhammad Wasif Gulzar*1, Riffat Maqsood1, Hussain Abbas1, Musharraf Manzoor1, Muhammad Suleman1, Hassaan Ahmad Bajwa2, Ali Hamza2, Shaher Yar1, Muhammad Zain1, Abdul Wadood1 and Noman Aslam3

...critical to manage plant pathogen-carrying insects and mites. Pesticides are useful in controlling vector populations because they limit the number of people who can contract and spread a virus, which may reduce the prevalence of sickness. Aside from their deadly toxicity, certain pesticides have characteristics that alter feeding patterns or otherwise impede the spread of viruses.

...

Daniel Offiong Etim1*, Etim Johnson Umana1, Idorenyin Asukwo Udo2, Ndarake Eden Ini-Ibehe1

...es and fungi are serious pathogens affecting okra production. It has been observed that okra production has not been maximized due to nematode and fungus infestation. A research into the control of Meloidogyne incognita and Sclerotium rolfsii were examined in screenhouse. One gram of Trichoderma viride at 2.40×107 CFU/g and Trichoderma harzianum at 1.40×107 CFU/g of millet were applied in combination with neem cake at 2.00 t/ha and 4.00 t/ha. Soil ...

Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3

...fy;">Tick and tick-borne pathogens are among the major threats to animal health and production causing huge economic losses in the form of morbidity and mortality throughout the world. Due to the scarcity of epidemiological data and appropriate diagnostic methods, the study was carried out to detect Anaplasma marginale and Theileria annulata from buffaloes of Sheikhupura, Jhang, and Rawalpindi districts by using duplex PCR. A total of 1,152 blood samples of bu...

Endy Triyannanto1, Danung Nur Adli2, Diah Pratiwi3, Lukman Hakim3, Selma Noor Permadi3, Taufik Kurniawan3, Angga Maulana Firmansyah3, Lina Ivanti3, Dinar Suksmayu Saputri3, Mohammad Miftakhus Sholikin4, Hari Hariadi5, Tri Ujilestari3, Teguh Wahyono3* 

...ugh it can decontaminate pathogenic microbes, gamma irradiation can affect the texture, change color, increase oxidation parameters, and reduce the sensory parameters of pork products.  

...

Suhair Sh. Al-Siraj1, Jihan M. Badr2 and Dalia M.A. El-Masry3*

...nanoemulsion may prevent pathogenic microorganism-induced deterioration and provide antibacterial agents for gram-positive and gram-negative bacterial infections.
 
Keywords | Plant extract, Bay leaf, Nanoemulsion, Antibacterial, Pathogenic bacteria, Gene expression, Resistance
...

Desak Putu Mas Ari Candrawati*, I Gede Mahardika, I Gusti Nyoman Gde Bidura, Ni Wayan Siti

...lood lipid profiles, and pathogenic bacteria in intestines. Synbiotic comprised a combination of prebiotics Moringa oleifera leaves and probiotics yeast culture in a ratio of 1:1 (g/g). The results of in vitro analysis contained probiotics Saccharomyces spp. of 1.7 x109 cfu/g. Broilers used were 200-day-old chicks (DOC), divided randomly into 4 treatments with 5 replications, each consisting of 10 broilers. The control (treatment A) was given basic feed withou...

Muhammad Sajid1*, Muhammad Hamid2, Usama Ayyub2 and Muhammad Azam2 

...limited understanding of pathogenesis and immunobiology, multiple routes contribute to the viral entry, replication, and clinical damage. A few autopsy reports have highlighted mention hemorrhages and extravasation involving many body organs. A deeper comprehension of the pathophysiology and immunology of the CCHFV virus will enhance patient care and hasten the creation of CCHF medication countermeasures. Physicians We anticipate that physicians should familia...

Ying Zhong1,3,4, Jingni Chen1, Chunping Huang1, Huaiyuan Jin1, Jinlu Huang1, Lining Zhao1, Yi Geng3, Guiping Wang1,4 and Xueqiao Qian1,2*

...ntified seven strains of pathogenic bacteria from ponds with outbreaks of acute hepatopancreatic necrosis at a L. vannamei farm in Zhuhai, China. Among the seven bacteria strains, one strain was isolated from aquaculture water, two strains were isolated from hepatopancreas, and the other four strains were isolated from juvenile shrimp. The results of 16S rRNA sequencing and biochemical identification showed that all seven pathogen

Umm-e-Kulsoom1, Saima Hashim2, Fazli Wahid3, Haseena Gulzar4 and Tamana Bakht5

...otato. There are limited pathogens or insect that can significantly affect the potato while presence of weeds is a major concern for the potato growers. As per our documentation survey, Amaranthus viridis and Chenopodum album were found as major weeds infesting potato fields. At maturity, the seeds of these weeds were collected and the major trees that were found on the sides of nearly all potato fields were identified as Juglans sp, Cedrus sp, and Pinus sp. T...
Sohaila Fathi El-Hawary1, Nermeen M.L. Malak2, Reda A. Gomaa3, Hesham Z. Tawfeuk3, Suzan Ismail4, Nady Khairy Elbarbary5*
... species are significant pathogenic bacteria found in fish that induce gastroenteritis, a major public health issue. The present research investigated the occurrence, virulence gene detection, antibiotic resistance profile, and the antibacterial influence of lemon juice and pomegranate peel extract on V. cholerae and V. parahaemolyticus. Samples were 150 fish (30 of each Nile tilapia, Nile perch, sardine, Mugil cephalus, and Sea bass) from fish s...

Afnan Ahmed Allhyani1, Mohamed Baeshen1, Nagwa Thabet Elsharawy2,3

...ffect against the tested pathogens. So, it can be a natural alternative to conventional formulations for those interested in naturally based products.
 
Keywords: Ethanolic extraction, Water extraction, Foodborne pathogens, Antimicrobial agents, Meat preservation, Goat meat
...

Ibrahim Ali1,2, Hanan Mohamed Fathy Abdien1*, Wael Kamel Elfeil1, Mohsen Mohamed Zaky ElDemerdash1, Mohamed Ali Zain El-Abideen2,3, Walid Hamdy Kilany2,3

...Seven groups of specific pathogen-free (SPF) chickens were treated as G1 and G2 vaccinated with (H5N8+Gel 01), G3 and G4 with (H5N8+IMS1313) by intranasal (IN) and intramuscular (IM) for each treatment respectively. Each group (G1-G4) was vaccinated twice at day 7 and 21 of age. Meanwhile, G5 treated with (H5N8+ISA70) received the primary vaccination dose intramuscularly only on day 7 of age. Group G6 served as a positive control (untreated and challenged), an...

Natalia Shchur1,2*, Tetiana Mazur1, Orest Katsaraba3, Ihor Halka2, Ludmila Shalimova2, Larisa Moskalenko4, Tetiana Ponomariova-Herasymiuk4, Maksym Lusta4, Vitalii Nedosekov1

...ad of Campylobacteriosis pathogens in Ukraine, revealing infection rates of 26.3% in broilers, 3.33% in turkeys, 55% in pigs, and 8.33% in cattle. In this research of the study, 33 isolates of Campylobacter spp. were identified for the first time, representing 23.9% infection, among which Campylobacter coli accounted for 75.8% and Campylobacter jejuni for 24.2%. Notably, up to 70% of poultry isolates were identified as Campylobacter coli. To improve the indica...

Bunga Rimta Barus1, Masfria Masfria2*, Aminah Dalimunthe3, Sovia Lenny4

...aphylococcus aureus is a pathogenic bacterium capable of causing wound infections. This study aimed to evaluate the effectiveness of gel formulations containing the ethyl acetate fraction obtained from red ginger leaves in facilitating the healing process of excision wounds. The study utilized 30 male Wistar rats, divided into six groups with five rats each, to ensure robust statistical analysis. The ethyl acetate fraction was extracted using a detailed macera...

Mohamed Ahmed Abaza1, Amany O. Selim2, Mona Abdallah3, Shimaa A.E. Atwa4, Hala El Daous5, Mona Abd-Allah Abd-Elrehim6, Mohamed M.S. Gaballa7, Reda R. Fathy1*

... in one day old specific pathogen free chicks to evaluate the antibacterial effectiveness of 50mg/kg ration dosage compared with colistin as commercial antibiotic at 5 days old. E. coli serotype O6 was the highest prevalent and pathogenic multi drug resistant bacterial strain.The assessment parameters were clinical signs, post-mortem lesions and histopathological picture which showed effective role of ZnO-NPs as bacterial in...

Enany M.E.1, Fadel Hanaa M.2, Abo-Shama U.H.3, Ahmed Mona M.1, Kholief M.E.A.4*

... common bacterial humans pathogen, possess virulence genes such as vacuolating cytotoxin gene A (vacA), cytotoxin-associated gene A (cagA) and, (hrgA) which have not widely recognized function. The aim of the current study to determine the efficacy of antibiotic therapies of human diseased animal model as felines and sheep infected with virulent H. pylori in seasons. A total of twelve H. pylori isolates cultivated from samples of stomachs and stool of felines,...

Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1

...f multidrug resistant uropathogenic E. coli (UPEC) which causes community-acquired UTI in pets and human community has been monitored. The current study aimed to investigate the prevalence of shared uropathogenic E. coli serotypes isolated from companion animals and human as well as assessed their virulence determinants which implemented in pathogenesis and severity of infection via phenot...

Yawar Abbas1, Muhammad Sajid2*, Musharraf Hussain3, Shahida Batool1, Saeed Ur Rehman1 and Jamil Ahmad1

... which appear to be more pathogenic and transmissible. While similar to smallpox virus, mpox virus was first reported in human during 1970 and since then remained endemic in Africa. First time in 2022, numerous cases were reported in various parts of Africa, as well as in the Northern and Western Hemispheres. Given the wider spread, changing dynamics and host spectrum, we aim to provide critical view of zoonotic nature of mpox virus in human, taxonomical posit...
Sultan Çobanoğlu1, Waheed Anwar2*, Muhammad Asim Javed2 and Hafiz Azhar Ali Khan3
...te the efficacy of entomopathogenic fungi using two concentrations (4×104 and 4×108 conidia/ml): Beauveria bassiana, Metarhizium anisopliae, Trichoderma longibrachiatum and Verticillium lecanii, against the adult female of T. urticae strains (red and green) using leaf-disc bioassay method. Their corrected mortalities were also calculated using Abbot formula. Results indicated that the concentration 4×108 conidia/ml of Trichoderma longibrachia...

Nahed Yehia1*, Rania I. Mohamed2

...stify;">However, the low pathogenicity of avian influenza (H9N2), can have significant economic losses in poultry farms associated with other viral and bacterial infections. The purpose of this research is to study the genetic evolution and pathogenicity of the H9N2 virus during 2022. The forty tracheal samples were gathered from broiler chicken farms from 5 governorates (El-Dakahlia, El-Sharqia, Alexandria, Al-Qalyubia, and...

Naglaa A. El-Taib1*, Asmaa T. Talayea2, Hanan R. Ghanayem3

...xt-align: justify;">Many pathogens, including Escherichia coli (E. coli), can enter a viable but non-culturable (VBNC) state in response to environmental stress. In this state, bacteria cannot grow on standard media but retain certain features of viable cells, such as cellular integrity, metabolic activity, and virulence. Therefore, this study examined the effect of chlorine and hydrogen peroxide as common chemical sanitizers on inducing E. coli O157 into a VB...

Shaymaa Jabbar Hassoon1*, Hiba Ibrahim Ali2, Ayat Jasim Mohammed3, Oras Salman4

...obial properties against pathogenic isolated bacteria and offering a novel way in the combat alongside bacterial contagions.
 
Keywords | Silver nanoparticles, Cinnamon bark, Fresh sheep meat, Antimicrobial activity, Staphylococcus aureus, Escherichia coli
...

Yasser Asaad Hameed Al-Shareef, Firas Hussain Kadim Abawi*

... determinant of ND virus pathogenicity by the analysis of the fusion protein cleavage sites, phylogenetic analysis sand compared genomics of NDV isolates to publish sequences. Isolates characterized here showed 90.37% sequence similarity to the Iranian isolate Asil/IR/AAA158/2019 (MN370894.1) within velogenic clusters. Taken together, clinical, genetics and molecular biological approaches identified velogenic strains of NDV in poultry which breach the immunity...

Jumiati Asis1, Nur Aainaa Hasbullah1, Mohamadu Boyie Jalloh1, Noor Khairani Mohamad Basri1, Palanivell Perumal2, Peter Mojiun2 and Mohd. Rashid Mohd. Rakib1* 

...inst various fungal phytopathogens. On the other hand, Ganoderma boninense has been identified as the phytopathogen causing basal stem rot (BSR), a devastating disease in oil palm crop (Elaeis guineensis). In this study, the in vitro and in planta inhibition of G. boninense using Trichoderma isolates were evaluated. A total of 20 Trichoderma isolates were collected. Among the ...

Qasim Jawad Amer, Wissam Ahmed Sabah Al-Janabi* 

...evansi) is a significant pathogen affecting camels arround the globe. The study aims to evaluate the potential of molecular methods as a reliable alternative to traditional techniques for accurate and rapid diagnosis of T. evansi in camel populations. For this purpose, this study was designed to collect seventy-five blood samples (n=75) from camels of different ages of both sexes (male and female) from different areas of northern and southern Babylon.Each bloo...

Sehar Aslam1, Muhammad Qasim1*, Mohsin Khurshid2*, Usman Ali Ashfaq1 and Muhammad Akhtar Ali3

...nst antibiotic-resistant pathogens, such as Escherichia coli and Staphylococcus aureus. The anti-cancer activity of all isolates was also evaluated against the Human colorectal adenocarcinoma cell line (Caco-2), and all isolates showed significant anticancer activity. These findings validate the beneficial therapeutic values of novel probiotic strains isolated from dairy sources, specifically Dahi, and suggest that they could be used in food and drugs to treat...
Mat Zin Ain-Auzureen1, Maizan Mohamed1, Tan Li Peng1, Mohd Faizal Ghazali2, Choong Siew Shean1, Kamaruddin Mardhiah3, Najiah Musa4, Nora Faten Afifah Mohamad5, Chai Min Hian2 and Ruhil Hayati Hamdan1*
... V. alginolyticus pathogenicity and evaluating antibiotics misuse for the development of sustainable disease control methods.
...

Ahmed E. Orbano1, Mohsen El Dimerdash1, Mohamed Abaza2, Ali Zanaty3, Mohamed Rady3, Mohamed H. Nemr3, Wael K. Elfeil1*

...g RT-PCR to detect viral pathogens. Results showed that most flocks had been co-infected with more than one viral agent and there were 24 positives (35%) for IBV, 39 positives (57%) for H9N2, 18 positives (26%) for NDV, and 9 positives (13%) for ILTV. The results revealed a high prevalence of mixed infections, with 52% of flocks co-infected by two or more viruses. The most common co-infections involved IBV with H9N2 (9%) and IBV with ILTV (9%). Genetic analysi...

Eslam Arafa1,2, Hanan M.F. Abdien1*, Mohamed Ali Zain El-Abideen3, Emad Diab2,4, Mahmoud Assad2,5, Mohamed Tarek3, Mohsen M.Z. El-Dimerdash1, Wael K. Elfeil1

...was isolated in specific pathogen-free embryonated chicken eggs (SPF-ECE) via the yolk sac and chorioallantoic membrane routes, resulting in embryonic mortality and hemorrhage with congested internal organs. Survivors exhibited liver necrosis. Hepatitis, myocarditis, and infiltration of heterophilic diverse inflammatory cells were recorded upon histological examination. These findings confirm that the mutant ARV can skip maternal immunity induced by convention...

Qasim H.A. Aljboori* and Saadoon Murad Saadoon

...ne of the most important pathogens that causes rot disease in rice and affects plant growth. Many Fusarium species can infect rice plants and affect its growth, in addition to producing various mycotoxins that are dangerous to humans and animals. Therefore, a study was conducted to evaluate the effectiveness of four levels of agricultural sulphur (0,100,200 and 300 g/m2)and the fungicide Benomyl (100 ppm) against F. solani fungus infecting three varieties of r...

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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