Beauveria bassiana as a biological control agent against forest pests Poecilocerus pictus (acrididae, orthoptera, and Brachytrypes portentosus (gryllidae, orthoptera)
Hanif Gul, Nighat Mansoor Chaughtai and Rukhsana Kausar
ABSTRACT
Entomopathogenic fungus Beauveria bassiana was tested as
biological control agent against forest nursery pests
Poecilocerus pictus, and Brachutrypes portentosus in the
laboratory at the Pakistan Forest Institute, Peshawar
under the controlled temperature of 25-35°C and 55-65%
relative humidity, for evolving their best possible
biological control. B. bassiana fungus was tested in the
doses of 0.8 x 104 spores/ml, 0.6 x 104 spores/ml in the
study. The data showed that B. bassiana has given a non-
significant result against the pests even after 72 hours
but results were significant after 96 hours upto 168
hours of treatment. The study showed that after
treatment the fungus first established and then caused
the mortality of Poecilocerus pictus within 2-3 days
while in case of Brachutypes portentosus within 7 days.
The fungal hyphae developed in the treated insect pests
first in their soft body parts like thorax and abdomen
covering the whole body within a period of two weeks.
The study showed the susceptibility of these insects to
the fungus B. bassiana which could be used as biological
control agent in the field.
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