Maureen McKeague1, Maria C. DeRosa2*
...ant new approaches and tools for SELEX. In this commentary, we discuss recent advancements in the selection of aptamers....
Nicola J. Stonehouse
...be useful as molecular tools and also as novel therapeutic agents. This commentary describes RNA aptamers selected to bind to the RNA-dependent-RNA polymerase of foot-and-mouth disease virus. The aptamers are able to inhibit the function of the enzyme both in vitro and in the context of a sub-genomic replicon and highlight the future potential of this technology in many aspects of virus research as well as in diagnostics and therapeutics....
Muhammad Ashraf Khan1, Hizbullah Khan1, Abid Farid2
...an) relative to the controls was less than 10% for all doses and life stages treated, indicated that HaNPV is harmless (E< 30%) to the emergence as well as parasitism efficiency of T. chilonis emerged from treated host eggs....
Faheem Khan*, Muhammad Zafarullah Khan
...ping special extension tools for those who cannot read....
Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman
E-mail | irfanmudasir@gmail.com
...er their respective controls at all doses and growth stages observed, attaining maximum with 40 grams Imidacloprid active ingredient per hectare-1 at day 15 after treatment. The antioxidative enzyme activity of SOD, APX and GR showed increasing trend in a dose-dependent manner, attaining maximum at 40 g.a.i ha-1 of Imidacloprid treatment at day 7 after treatment (DAT) followed by 3 and 15 DAT, respectively. On the contrary, the CAT activity was increased at lo...
Ashley C. Banyard1* and Satya Parida2
...by the OIE. Whilst the tools are available to attempt eradication there are a number of factors that will influence the strategy taken to progress with the aim of eradication. Here we describe current areas where knowledge improvement is required to shape our understanding of the disease and its distribution alongside areas of investigation that require consideration in any viral eradication campaign. ...
Zahir Shah, Shakoor Ahamd, Mian Saeed Sarwar, Murad Ali Khan and Javid Ali
...in four weeks old cross Holstein Friesian dairy breed calf with hanging mass in the umbilical region. Tentative diagnosis was made through clinical sign and physical examination; the area was incised under local anesthesia. After surgical procedure site was closed by applying mattress sutures. Adjunct therapy of broad-spectrum antibiotic was administered intramuscularly for five days as a post-operative measure. Catamnesis revealed that the healing was complet...
Tarun Kumar Sharma, John G. Bruno and William C. Cho
...emerged as competitive tools with the potential to replace antibodies in many diagnostic formats. The aptamer industry is continuously growing to create space in research and diagnostics markets. However, translation of aptamer in several applied disciplines is still lagging behind as compared to antibodies. In this article, we discuss the translational aspects of aptamers, current potential of aptamers and strategies to create the “bench-to-bedside&rdqu...
Aqila Shaheen and Mehwish Matien
...soil groups were Inceptisols (site 1) and Mollisols (site 2). Soil samples were analyzed for bulk density (BD), soil organic carbon (SOC), total nitrogen (TN), available phosphorus (AP), extractable potassium (K) and pH. Statistically (p ≤ 0.05) higher SOC contents of Inceptisols and Mollisols were 14.18 g kg-1 and 23.42 g kg-1,&nb...
Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*
... normal age matched controls were examined. Genomic DNA was extracted and amplified by PCR using specific primers for GPR54 and GNRHR splice site exons. Mutations were analyzed by single-stranded conformation polymorphism (SSCP) and/or sequencing. No mutation was identified in GPR54 gene, while two mutations in GNRHR gene were observed in one sporadic case of isolated HH. The first was a synonymous substitution mutation of T to G at nucleotide position 123, wh...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...re used as positive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorganisms, while C. sinensis and M. piperita showed weak antimicrobial activity. Methanolic plant extracts exhibited greater antimicrobial activity than aqueous extracts. Maximum zone of inhibition exhibited by meth...
Zaffar Malik, Moazzam Jamil, Ghulam Hassan Abassi, Muhammmad Nafees, Muhammad Rafey and Muhammad Kamran
...ical parameters of vertisols. However, low doses (2%) of straw biochar were more beneficial by keeping in view the availability and economic level of the landholders....
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
...virus. Out of total 50 pools of Aedes aegypti larvae, DV-2 was the most prevalent serotype. DENV-1 was not found in any pool. While all pools of Aedes albopictus were negative for dengue virus. Our study showed that the main cause of spreading of dengue virus was Aedes aegypti. Serotype DENV-2 was dominant in the field collected larvae of Aedes aegypti in Lahore and Sheikhupura, Pakistan.
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...better vaccination protocols and infection control in the future....
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
...17.14%) adult mosquito pools were found positive for dengue virus with type-2 (77.77 %) and type-3 (11.11 %) while a single (11.11%) pool of concurrent infection with type 2 & 3 was also detected. The adult Ae. aegypti belonging to Shuba Bazaar showed 33% positivity for DENV with minimum infection rate (MIR) 11.1 while that of Wazir Bagh showed 16.67% positivity with MIR 5.5. Similarly, the Ae. albopictus of Shuba Bazaar resulted in 22.22% po...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... studies are effective tools that could be used as complementary in diagnosis of amphistome infections in ruminants.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...molipidemic healthy controls and 102 patients with hypercholesterolemia in a Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers hav...
Muhammad Zafarullah Khan
...pments and use of hand tools and implements. Most of the respondents have high competency level having practical farming experience. Study suggests pre-service along with in-service trainings along with higher degree in agriculture i.e. PhD for improving their technical competencies in agricultural mechanization...
Usman Shakoor1*, Mudassar Rashid1, Abdul Saboor2, Nabila Khurshid1, Zuhair Husnain2 and Abdul Rehman2
...cipitation are helping tools to forecast scenarios for the climate in an area. Changes in these tools from the past due to any unavoidable circumstances for today and in the future is refers to climate change (CC). Besides other living being, crops are either benefited or adversely affected by these changes in the climate of an area. Maize is one of the widely grown crops in the world and even in Pakistan has also influenced...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...ated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...n the use of molecular tools. In this study, we analysed the G-protein-coupled chemokine receptor (GPCR) genes of two LSDV isolates from Ixodid (hard) ticks (Amblyomma hebraeum) in Egypt. Multiple alignments of the nucleotide sequences revealed that both isolates had nine nucleotide mutations in comparison with the local reference strain, LSDV-Egypt/89 Ismalia. Compared with the GPCR sequences of SPV and GPV strains, 21 nucleotide insertion and 12 nucleotides ...
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
...ffect of different protocols of alfalfa hay supplementary on ruminal characteristics and performance of growing Naemi lambs fed TMR. Four groups, each of 3 months old growing Naemi male lambs (28.85±1.09 kg), were fed on TMR (NDF=41. 95%) (T1). TMR plus 100 g alfalfa hay (NDF= 43.3%) daily (T2), TMR plus 200 g alfalfa hay (NDF= 43.3%) every two days (T3) and TMR plus 300 g alfalfa hay (NDF= 43.3%) every three days (T4). Rumen fluid samples were analyzed...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...SM-IV) criteria and controls (n=120) were selected from the same ancestry during August 2011 to January 2014fromSir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore. The demographic values for age, employment and marital status did not differ significantly among case and control groups. Exonic regions of BDNF gene were sequenced. In-silico analysis was performed to detect the possible disease causing effect of genetic variants with OCD. ...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...compared to healthy controls. HLA-DRB1*11 demonstrated significant association with aeroallergens and could have a protective role for allergy while HLA-DRB1*12 did not show any association with aeroallergen sensitization.
...
Muhammad Imran Shabbir
...es along with gender symbols and affection status are used to modify the Punnett squares. On applying these modified Punnett squares along polygonal shapes covers all possible parental combinations and their genotypic and phenotypic outcomes in offspring. In this way, simple illustrations incorporate a considerable amount of information that provides the opportunity to determine genotype and phenotype probabilities for monogenic Mendelian traits without the ne...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...tified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3
...ecticide which also controls bollworm effectively and relatively safer for natural enemies and beneficial arthropods. Present investigations were carried out to determine their impact on larval mortality, pupal and adult emergence of 2nd and 4th instar larvae of Spodoptera litura F. under laboratory conditions. Both NPV and flubendiamide were applied using diet incorporation method. Larvae were allowed to feed for 48 h. Mortality w...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...markers will be useful tools for investigating genetic diversity and population structure of this species.
...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...SM-IV) criteria and controls (n=120) from Sir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore from August 2011 to January 2014. During the sample collection the factors like age/period, employment status and marital status were considered after informed consent. We found one single base change G>C at c.745 resulting in a nonsynonymous change p.E249Q in theCOMT gene. In-silico analysis predicted it to be damaging and disease causing. Screeni...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...ed using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60oC, Protocol III (concentration of dye in staining solution); slides were stained with 1, 5, 200, 500, or 1000µg/mL solution. The slides were observed, intensity of sta...
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...ples (diabetic=451, controls=449) were genotyped by PCR-RFLP. A significant difference in genotype distribution was observed between cases (Gly/Gly, 66.9%; Gly/Ser, 20.17%; Ser/Ser, 12.8%) and controls (Gly/Gly, 67.4%; Gly/Ser, 28.5%; Ser/Ser, 4.00%) (p = 0.013). The cases showed significantly higher frequency of the Ser allele compared to the controls (22.9% vs. 18.3%; OR: 1.32, CI...
Ambreen Butt1, Uzma Malik2, Khadija Waheed3, Amna Khanum3, Samar Firdous4, Sara Ejaz5 and Fawad Randhawa2, Tania Shakoori6*
...s (GDM) and healthy controls and to propose a cut off value for serum B12 levels as a predictive biomarker for GDM. Study design was Quantitative cross-sectional study. This study was carried out at Lady Atchison Hospital/Mayo Hospital Lahore, Pakistan from January to December 2016. Cobalamin levels were measured in women diagnosed with GDM (n=59) and controls (n=41). GDM was diagnosed when fasting blood sugar levels were 95...
Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2*
...ity. In this study, 59 pools of intestinal content of layers, broilers, and breeders were investigated from different Brazilian regions for the presence of rotavirus (RVA, RVD, and RVF), avian coronavirus (ACoV), and avian astrovirus (AAstV), using PCR reactions followed by nucleotide sequencing and analysis. Twenty-nine pools (29/59; 49%) appeared positive for AAstV, 6 pools (6/59; 10%) w...
Rana Muhammad Aadil1,2*, Xin-An Zeng1, Saqib Jabbar3, Akmal Nazir2, Abid Aslam Mann2, Muhammad Kashif Iqbal Khan2, Abdullah2, Amna Ramzan2
...ids (TF) and total flavonols as equated to thermal and control, while ºBrix, pH and acidity remain unchanged in all HPP treatments with control and thermal. After this, we suggested that HPP treatment (250 MPa/60 °C/3 min) has a potential against thermal to progress the value of grapefruit juice and could be applicable at industrial scales.
...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...cattle. Group A was controls: Animals in Group B and C were at 45 days prepartum with and without VES: animals in D and E were at day 60 prepartum with and without VES: animals in F and G were at 75 days prepartum stage with and without VES: respectively. At day 0 of experimental trial VES was given to animals in group C, E and G. All groups were given oil based HS vaccine at day 15 and alum based FMD trivalent vaccine at day 30 of experimental trial except Gr...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...ice, as compared to controls when placental lactogen was applied. Increase weight gain for visceral tissues like kidney, liver, muscle and lungs was also observed, as compared to control subjects. It was also recorded that the exogenous supply of bubaline PL reduces fat cells in the hypodermal layer of skin.
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