Effect of Inducement, Dilution Ratio and Freezing Rate on the Quality of Cyprinus carpio (Linnaeus, 1758) Spermatozoa
Effect of Inducement, Dilution Ratio and Freezing Rate on the Quality of Cyprinus carpio (Linnaeus, 1758) Spermatozoa
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
ABSTRACT
Cyprinus carpio is a widely cultured species and exploitation of natural stock due to increased demand in production can be averted by hormonal inducements which further affects the quality of gametes. Cryopreservation can aid in the conservation of gene pool and bestow superior quality sperm. Freezing and thawing rate are the most critical factor in the cryopreservation of milt. In the present study, variability between spermatological parameters of induced and non-induced C. carpio was documented for a period of 7 months. Further, the non-induced milt was stored for 24 h at three dilution ratio (1:10, 1:20 and 1:40) and spermatological parameters were analysed. Then the milt was cryopreserved with the best dilution ratio using 3 freezing protocols with two step cooling profile in programmable freezer. The spermatological properties of milt obtained from non-induced fishes were superior which was further used for short term preservation. When experimented with dilution ratio, the highest motility duration of 74.3±2.16 s was obtained at 1:10 dilution ratio. The best results on motility duration (62.28 ± 2.12 s), fertilizing ability (77.3±1.63 %) and hatching rate (61.6±2.44 %) was recorded when freezing protocol I was followed and the values were statistically significant. Hence the results revealed that for successful cryopreservation of C. carpio milt, use of non-induced milt at 1:10 dilution ratio using slow freezing rate will give the best results in terms of motility, fertilization and hatching rate.
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