Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

... the first report of the isolation and molecular identification of Mmm from the ear canal of cattle in Nigeria. It is recommended that samples from the ear canal be considered when Mmm isolation is intended so as to improve the chance of recovery. 

...

Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2

...s were processed for RNA isolation and subjected to molecular detection of H9 gene of H9N2 subtypes using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Out of 24 samples taken from each organ, 22, 21, and 18 samples from trachea, lungs, and intestine showed viral RNA, respectively. Taken together, results showed that the H9N2 is endemic and widely distributed in asymptotic layers. Furthermore results indicated that H9N2 subtype may survive...

Ibrar Khan1,2,3,*, Sadia Qayyum2, Shehzad Ahmed2, Kashif Syed Haleem2, Mujaddad-ur-Rehman1, Guang-Lei Liu3 and Zhen-Ming Chi3

...ily. The study comprised isolation, microscopic observations and ribotyping, allowed discrimination of the marine fungal isolates as Penicillium viticola F1, Penicillium restrictum F2, Penicillium rubens F8, Penicillium implicatum F10, Penicillium piceum F11, Penicillium oxalicum F12, Penicillium sumatrense F15, and Penicillium vinaceum F16. This study demonstrated that Yellow Sea can be considered as a valuable natural source of Penicillium species, which can...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...es on the separation and isolation of potent metabolites is suggested to get a useful lead structure for development of novel antibiotics to combat resistant pathogens.

...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*

 Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy

... respectively. Bacterial isolation and characterization were performed in accordance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds all four pathogens disseminated in various organs with higher incidence of mixed compared to single infection. Different serotypes of E.coli and Salmonella could be recovered from dead birds however E.coli (O7...

Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1

...ndoff having the highest isolation rate in all the matrices sampled (58.8%), followed by, S. Kentucky (17.7%), S. Schwarzengrund and S. Havana had the lowest isolation rate (11.8%). S. Llandoff was isolated in all the matrices and was distributed across the three LBM whereas the other less frequent serovars had a more circumscribed distribution. Resistance to Methicillin, Penicillin, Erythromycin and Oxacillin was 100% for a...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...al farm, using classical isolation and compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. using brain heart infusion broth supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples wer...

Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram 

Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...regions of Karnataka for isolation of chilli microflora. From these samples, 94 fungal and 89 bacterial isolates were derived, which included 50 isolates of fungi and 44 isolates of bacteria from phylloplane and pomoplane, 44 isolates of endophytic fungi and 45 of endophytic bacteria from inside the leaf and fruit tissues, respectively. Among them, 70 fungal isolates belonged to plant pathogenic genera like,Alternaria, Cercospora, Colletotrichum, Curvularia, G...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...s of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the rate of 0.1ml of EID50 (virus titer 105.50/100μl) on 2nd week of age through eye drops. Group B specific antibo...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...x C-25) was used for the isolation of lactoferrin using the fast protein liquid chromatography. The lactoferrin was further characterized through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 ...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...region were analyzed for isolation. Isolation was carried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. The total prevalence recorded was 3.66%and ...
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
...present study deals with isolation, identification and characterization of some indigenous heavy metal resistant bacteria isolated from rhizosphere of Tagetes minuta, growing in soil adjoining automobile workshops to investigate their bioaccumulation capacity for three selected metals Cr(VI), Ni(II) and Cd(II). The plant was selected due to its natural abundance in the climate of Kashmir and also in the area of study. On the basis of morphological, bioc...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
...s using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.201 to 0.630, respectively. The polymorphic info...

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

...teriological studies for isolation and identification of Brucella organisms. Brucella melitensis biovar 3 could be recovered from 64 (61.5%) tissue specimens and 28 (60.9%) milk samples. Brucella cultures were further identified on molecular basis using universal and Bruce ladder PCR. 

...
Anan Kenthaoand Pornpimol Jearranaiprepame*
...uld define relied on the isolation of river drainage. Moreover, the study also indicates the involvement of environmental conditions in morphological adaptation, providing useful information for the sustainable conservation of this fish.
...
Ikramul Haq1, Aneela Zameer Durrani*1, Muhammad Sarwar Khan1, Muhammad Hassan Mushtaq2, Imtiaz Ahmad3, Amjad Khan2 and Mehboob Ali1
...c foals were sampled for isolation of bacteria. During the study 294 faecal samples (horse n=217, mules n=23 and donkey n=54) were diagnosed positive for bacteria. The overall prevalence of diarrhea was 72.56% with bacteria in 65.77% of diarrheic cases. The prevalence of different bacterial species shows that E. coli was the most prevalent bacteria with 48.77% as compared to Clostridium perfringens (18.56%) and Salmonella (17.9%). It was c...

 Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***

CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...ective of this study was isolation and characterization of bacterial strains from rhizosphere, endosphere and rhizoplane of sunflower. Thirty six bacterial strains were selected out of 44 from plant root samples along with rhizospheric soil, collected from different salt affected areas of Central Punjab (Pakistan). Selected bacterial strains were characterized morphologically as well as biochemically at National Agricultural Research Centre, Islamabad during 2...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...n the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showed 58.93% (264/448) and 37.23% (542/1456) seropositivity, respectively. A total of 17.1% (402/2341) samples collected from all these CRD-suspected birds were found positive on modified fray’s ...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...ere screened using virus isolation, as well as antigenic and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. De...
Leila A. Kaimbayeva1,*, Elena S. Malysheva2, Rashit Kazikhanov3 and Saule R. Kazikhanova4
...mmediately processed for isolation of muscular fibers and were evaluated for the pH, water-binding power, activities of tissue enzymes and microstructure changes. The results of chemical analysis revealed the nature of protein substances, altered рН value of the meat and the intensity of glycolysis. All these parameters were indicative of autolysis process in the meat. The levels of water-binding capacity and structural-mechanical properties of red...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...urther processed for the isolation of virus by embryonated egg inoculation technique. Moreover, out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14%), duck (n=5; 2%), migratory water fowl (n=3; 1%), pheasant (n=2; 0.8%), grey leg goose (n=1; 0.4%), turkey (n=1; 0.4%), eagle (n=1; 0.4%) and crane (n=1; 0.4%), for domest...

Xiangbin Meng1,Tianxiang Gao2, Chunhou Li3, Na Song1, Lu Liu1, Liqin Liu2 and Xiumei Zhang1,*

 

Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...oring and optimizing the isolation and culture methods of chicken amniotic fluid stem cells (CAFSCs) in vitro, characterizing CAFSCs and inducing CAFSCs to differentiate into three layers. Methods and results include that more CAFSCs are gained via thermoelectric method with lower cost. Groping and optimizing culture system abiding by the idea of simulating the chicken body environment, involving adding proportion of chicken serum and some growth factor...
Fouzia Hadi Ali1, Farhat Naz2, Aban Abid Qazi3
 
...s futile to study WLB in isolation, the literature suggests that positive psychological capital (PPC) triggers WLB. Moreover, WLB also assists in enhancing vitality at work that in turn improves WP. Therefore, this study intends to examine the sequential mediation of WLB and vitality at work between the relationship of positive psychological capital and WP.
Methods: A cross-sectional study was conducted at 80 hospitals in the province of Pun...
Hanif Ur Rahman1, Umer Saddique1, Zahoor-ul-Hassan1, Shakoor Ahmad1, Muhammad Kamal Shah1, Said Sajjad Ali Shah1, Farhan Anwar Khan1Fazli Rabbani2, Muhammad Asif Hussain2, Attaur-Rahman2, Irshad Ahmad3,4 and Sadeeq ur Rahman2,*
...ganism (PPLO) medium for isolation and identification of Mycoplasma species. Confirmed mycoplasma isolates were subjected to species specific PCR to identify members of MM cluster. Results revealed that 79/300 (26.3%) samples were found positive for the growth of Mycoplasma. MM cluster specific PCR showed 49 (16.3%) samples were found positive, of which 34 (11.3%) were found positive for Mycoplasma mycoides subsp. capri (Mmc)...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...ve experienced a genetic isolation or inbreeding. We assessed the level of genetic diversity and genetic isolation of different langur populations using Randomly Amplified Polymorphic DNA (RAPD) genetic markers. We collected 86 different samples (feces 64, hair 13, blood 5, tissues 4) from 5 geographic langur populations of Pakistan and Azad Jammu and Kashmir (AJK) and succeeded in extraction of DNA from 23 samples, which we...
Ayesha Aihetasham, Saira Shariq and Javed Iqbal Qazi*
... study was conducted for isolation and identification of fungal species from cadavers of notorious subterranean termite, Heterotermes indicola. Virulence of Rhizopus stolonifer isolate and resultant variations in food consumption of H. indicola workers were evaluated over a period of one month by employing surface culture method. Following different time exposures, R. stolonifer significantly produced epozootics on the tested insect...

Zulqarnain and Zafar Iqbal* 

...icillium sp. extract for isolation and structural characterization of its antifungal compounds. 

...
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
...arly. The possibility of isolation of fewer pathogenic agents in this study cannot be ruled out. There has not been any report of zoonotic infection in ornamental fish handlers in Lahore. There is one message that it should be assumed that zoonotic organisms are always present and safety measures should be used to limit the spread of these infections. Potential risk of zoonotic infections through contact with ornamental fish is discussed.
...
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...further investigated for isolation of S. aureus using standard procedures. Coagulase gene was PCR amplified from the clinical isolates to categorize them into Coagulase positive Staphylococci (CPS) and coagulase negative Staphylococci (CNS). A questionnaire was used to record risk factors associated with occurrence of SCM and results were analyzed using non-probability statistical analysis. Results indicated that an overall 63.28% (243/384...

Dilara Abbas Bukhari1, Abdul Rehman2*

Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus

Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1

Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
... /> Somatic cell count, isolation, identification and purification of microbiological
cultures. The mean score of surf field mastitis test of G1 group before treatment
and at day 14thpost calving was significantly different (p<0.05) as compared to G2
group Somatic Cell Count was significantly reduced from 8.0 at dry off to 3.50
(P<0.05) after day14th post calving in lispiracin™ group. Quarter wise prevalence
(%) ...

 Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1

Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...ed with cooking oil. For isolation
of virus, 21 samples were collected, out of which 8 (40%) were positive for H9. The
virus titer was ranged from 1:64 to 1:512. The body weight gain of FO
supplemented broilers was higher than broilers receiving cooking oil (CO) while the
FCR of FO treated broilers was significantly lower than broilers receiving diet with
CO before virus inoculation, furthermore a significant difference was o...

Aisha Siddiqua1*, Munir Ahmad1 and Nusrat Habib2 

...adaptation strategies in isolation or in combination on yield. The primary data of ‘Climate Change Impact Survey 2012-13’ used for this purpose. Crop-specific strategies of change in the time of sowing, use of inorganic fertilizer, change in irrigation, and varietal change. Change in irrigation practice gave rise to the highest yield suggesting that this might be the necessary requirement to sustain wheat growth in Pakistan in future. Farmers are e...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...s show that geographical isolation, especially by mountains, has played an important role in limiting rice frog migration.
...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

... centrifugation and then isolation of total RNA including miRNA was done. About 100ng of isolated miRNA was converted to cDNA after addition of poly (A) tailing and then expression profiling was done by using real time-qPCR technique in which miR-specific DNA primers were used to increase sensitivity of reaction. Expression of miRNA panel was normalized with mir-16 and expression fold change was calculated by 2-ΔΔCt method. Further analy...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

...samples for confirmatory isolation of Staph. aureus as a bovine mastitis causing agent. These Staph. aureus isolates were further tested for antibiotic susceptibility. Epidemiological data was analyzed by chi-square methods at 95% confidence interval. The overall prevalence of subclinical mastitis as a result of surf field mastitis test was 42.2%. The prevalence of disease was higher in cows (54.5%) than in buffalo (22.9%). Based on chi-square an...

Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1* 

...DV on the basis of virus isolation and haemagglutination assay (HA). Our results revealed that pH of the manure from time of its mixing with the NDV infected allantoic fluid till day 4 was 7, then pH values changed progressively and recorded 8.5 on day 12. After that, pH increased towards the alkaline side and recorded pH = 9 till day 33 of the experiment. Regarding moisture %, after mixing the manure with allantoic fluid the moisture % of the mixture was 67%,...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...y was to investigate the isolation, cultivation, identification and differentiation potential of Muscle-derived stem cells (MDSCs) from sheep skeletal muscle and provide an experimental evidence for clinical application. The skeletal muscle tissue were studied from fetal sheep, and the stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverte...

Fady Samir1, Rania F. El Naggar2, Mohamed M. Hamoud3, Manal M. Zaki1, Abdulrhman M. Gamal1, Samah E. Laban1, Shaimaa A. E. Nasr1, El Shaimaa Ismael1, Osama K. Zahran1* 

...ed on the basis of virus isolation followed by haemagglutination assay (HA) which revealed that NDV survived in the manure up to 28 days. Likewise, monitoring for the manure pH and moisture % was carried out. Our explanation for the long-time course for NDV persistence due to increasing the moisture %. In this context, the findings of this study revealed that genotype VII viruses of class II NDV are circulating in Egypt. Furthermore, genotype II-based vaccines...

Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1 

... areas of Tando Jam. The isolation from rhizospheric soil was done by dilution plate technique. Among isolated isolates, 6 representative colonies were purified, multiplied and characterized using light microscopy and other biochemical tests. The bacteria were tentatively identified as Pseudomonas, Streptomyces, Bacillus and Micrococus. These bacteria were checked for their antagonistic activity against Fusarium oxysporum f.sp. alium cepa. They all were found ...

Muhammad Rehan1, Asim Aslam1, Muti-ur-Rehman Khan1, Muhammad Abid2, Shakeel Hussain3, Javeria Umber4, Ahsan Anjum1 and Altaf Hussain2* 

... in Pakistan. Continuous isolation of new genotypes in Pakistan shows the evolving nature of the virus and the emergence of new strains is limiting in its diagnosis and control. Pakistan is a developing country and poultry industry is the backbone of its economy. Recent outbreaks have caused huge losses to the poultry industry. This review details the possible role and potential of some common captive and non-captive wild bird species in the spread of NDV and ...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...thods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by disk diffusion method. Double-disk synergy test (DDST) was also performed for det...

Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan 

...ustify;">Since the first isolation of the H9N2 low pathogenic avian influenza virus in 2011, the virus has distributed rapidly and widely in different poultry sectors in Egypt causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, 1026 confirmed positive H9N2 cases by RT-PCR out of 23182 different examined samples of different species and sectors in Egyp...

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...ustify;">Since the first isolation of the H9N2 low pathogenic avian influenza virus (LPAI) in 2011, the virus has been distributed rapidly and widely in different Egyptian poultry sectors causing severe economic losses and the problematic situation in poultry production especially with a co-infection with other circulating pathogens. In this study, a total of 23182 cloacal and tracheal samples were collected from suspected cases between 2015 and 2016 and su...

Sadaf Rahim and Mudassar Iqbal* 

...ther studied towards the isolation and structural elucidation of lead compounds is recommended for the development of local agro-chemical product as environmental friendly biodegradable organic insecticides. 

...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

... exploited for bacterial isolation and the isolates were qualitatively screened for proteolytic and lipolytic activity with skim milk and Tributyrin agar (TBA plate) assay method. From the collected samples, a total of 20 bacterial isolates (PAK-01 to PAK-20) were exhibiting psychrotrophic physiology. About 8 isolates were capable of proteolysis in alkaline range of pH; and 6 bacteria exhibited lipolysis. Based upon primary and secondary qualitative screening,...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...strumental in future for isolation of MAP to make vaccine against paratuberculosis using local isolates.
...

Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3 

Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
... the first to report the isolation of two B. canis strains from pet dogs in Sichuan province, southwestern China, and to further evaluate B. canis antigen location in tissues. Our study will contribute to the understanding of B. canis pathogenicity in naturally-infected pet dogs.

...
Beenish Zahid*1,2,7, Javed Iqbal Qazi2, Amir Zohaib2, Asim Aslam3, Raheela Akhter3, Haleema Sadia4, Qurat ul Ain5, Razia Sultana6, Irfan Irshad3 and Sobia Alyas7
...n inhibition test. Virus isolation rate of NDV from cloacal swab was 12% (6/50) and from oropharygeal swab was 8% (4/50). The 238-bp region of F gene of NDV was amplified by using specific primers of reverse transcriptase-polymerase chain reaction (RT-PCR). Out of 50 field samples 11 cloacal swabs (34%), were positive and out of 50, nine oropharyngeal swabs (18%) were positive for NDV through RT-PCR. DNA sequencing was performed by amplifying the RT-PCR amplic...
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
Abdul Samad1, Raheela Asmat2, Muhammad Naeem1, Hamida Ali3, Mohammad Zahid Mustafa1, Ferhat Abbas1, Jannat Raza1,3 and Muhammad Tauseef Asmat1*

 

Muhammad Israr1*, Helen Ross2, Shakeel Ahmad3, Nafess Ahmad4 and Urooba Pervaiz5

... to this poverty include isolation and remoteness of the area, low infrastructure, and poor access to markets and to social and communal services. The study suggests that multi-sectorial interventions and investment in terms of education, infrastructure, environment, financial services and agriculture development are necessary to overcome the situation and improve the likelihood of achieving the targets of the SDGs. 

...
Muhammad Younis Laghari1,2, Zixia Zhao1, Yiwen Gong3, Punhal Lashari1,2, Shangqi Li1, Jian Xu1 , Ateeque Rahman Khooharo4, Jiongtang Li1,* and Yan Zhang1*
...to the geographical isolation of Hainan island from the mainland, the genetic diversity in Hainan island is different to that in the mainland. One of fifty sequences isolated from SINE element was polymorphic, including six haplotypes with an average sequence divergence of 11.03% (SE = 0.78%). Four haplotypes (MF177501-MF177504) were shared in these four population. However, two specific haplotypes (MF177499-MF177500) were only observed in Hainan populati...

Muhammad Rasheed*, Tayyaba Naseer, Asma Hassan, Fayaz ul Hassan, Rifat Hayat, Ghulam Jilani, Samman Gul Vaseer and Muhammad Bilal Ali 

Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...ch is recommended on the isolation of the active ingredients of I. stolonifera for use in effective control of mosquito.
...
Mohammad Ejaz1, Salma Javed2, Muhammad Hamza1, Sadia Tabassum2, Muhammad Abubakar3, Irfan Ullah4*
... used endophytes for the isolation of various different types of bioactive molecules. These bioactive molecules can be used as antimicrobial, antibacterial and anticancer agents. Fungal endophytes like Taxomyces andreanae isolated from Yew plant have the potential to produce an anti-cancer compound called Paclitaxel. Other different fungal endophytic species produce various other types of anticancer compounds like camptothecin, podophyllotoxin, torreyan...

Adewumi Olubusuyi Moses1,2*, Faleye Temitope Oluwasegun Cephas,1,3, Ope-Ewe Oludayo Oluwaseyi1, Ibitoye Ibipeju Henrietta1, Arowosaye Abiola Opeyemi1, Adelowo Oluwatosin Deji1 and Adeniji Johnson Adekunle1,2,4 

...oupling Enterovirus (EV) isolation in RD and MCF-7 cell culture with direct detection from clinical sample might impact our ability to detect non-polio EV Species C (NPESC) members in stool samples. In all, 20% (8/40) of all the samples screened had NPESCs and 50% (8/16) of the EVs detected were NPESCs. However, the RD cell line missed all NPESCs. Hence, RD cell line alone should not be used for EV isolation if the desire is...

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

Sana Khan1, Raheela Taj1, Noor Rehman2, Asad Ullah3, Imad Khan3 and Sadeeq ur Rahman3,*
...ar, and processed by for isolation of C. freundii followed by phenotypic detection of β-lactamases and antimicrobial susceptibility profile. Our results indicated that a total of 130(4.40%;130/2950) samples were positive for C. freundii comprised of urine 58.46%, pus 33.85% and wounds 7.69%, respectively. More samples from females (n=70) than males (n=60) with majority (43.08%) from age group 21-40 years were found positive for...
Shaheen Rahman1, Kafeel Ahmad1*, Niaz Ali2, Muhammad Idrees3 and Waqar Ali4
...tudies were aimed at the isolation of A. tumefaciens from local soil. Five soil samples were collected from research farm of Agricultural University Peshawar, Pakistan. Soil samples were processed for the isolation of A. tumefaciens. Various biochemical and morphological tests were conducted to confirm the identity of A. tumefaciens. The pathogenicity of the isolates was confirmed by in vitro carrot and ...

Numan Ahmad, Shahid Bashirand Shahid Rauf 

DESIGN OF MINITURISED MIMO ANTENNA WITH W-LAN BAND NOTCHED FOR ULTRA WIDE BAND (UWB) APPLICATIONS
...ching as well as desired isolation. W-LAN notched band in the entire Ultra Wide band is obtained by etching a couple of straight line on the ground plane. A very compact UWB-MIMO antenna with overall dimension of 20×20 mm2 has been proposed which is smaller than most of other MIMO antennas designed for UWB. Impedance bandwidth for the proposed MIMO antenna is 2.9-11.1GHz with -10dB refection coefficient and isolation b...

 Numan Ahmad1, Shahid Bashir1*, Shahid Rauf1

DESIGN OF MINITURISED MIMO ANTENNA WITH W-LAN BAND NOTCHED FOR ULTRA WIDE BAND (UWB) APPLICATIONS
...ching as well as desired isolation. W-LAN
notched band in the entire Ultra Wide band is obtained by etching a couple of straight line on the ground plane. A
very compact UWB-MIMO antenna with overall dimension of 20×20 mm2 has been proposed which is smaller than
most of other MIMO antennas designed for UWB. Impedance bandwidth for the proposed MIMO antenna is 2.9-
11.1GHz with -10dB refection coefficient an...

Azam Khan*, Irshad Ahmad**

TIME HISTORY ANALYSIS OF SEISMICALLY ISOLATED FIXED BASE OFFSHORE PLATFORM
...e application of seismic isolation to a fixed-base offshore platform. A series of nonlinear time history analyses has been undertaken for the Platform A to assess structural integrity under Ductility Level Earthquake (DLE) design events. A full nonlinear time history analysis (including soil and foundation modelling) has been undertaken using finite element software SAFJAC for an extreme earthquake records scaled for the DLE event.
...

Muhammad Riaz1*, Naureen Akhtar1, Salik Nawaz Khan1, Muhammad Shakeel1,2 and Ateeq Tahir1

Neocosmospora rubicola: An Unrecorded Pathogen from Pakistan Causing Potato Stem Rot
... of same symptoms and re-isolation of N. rubicola from infected tissue confirmed Koch’s pathogenicity postulates. Association of N. rubicola causing stem rot in potato is never reported before in Pakistan.

...

 Muhammad Qasim Khan1*, Syed Athar Masood1 and Shahzad Naeem Qureshi2

REFORMULATION OF PRODUCTIVITY IMPROVEMENT MODEL FOR PUBLIC SECTOR AUTOMOBILE REBUILD ORGANIZATIONS
...d practices in piecemeal/isolation may not accrue true and optimal outcomes. The findings of this research will facilitate top and middle management to enhance productivity and output of public sector automobile rebuild organizations of developing countries in general and Pakistan in particular.

...

Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5

Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
...overall Mycoplasma bovis isolation rate of 0.83% (4/480) was obtained. Based on the states studied, 1 (0.35%) and 3 (1.53%) M. bovis were isolated from Adamawa and Taraba States, respectively with an insignificant association between M. bovis infection and the states sampled (P>0.05). Based on organs/site sampled, 2 (5.40%) isolates of M. bovis were from lung tissues and 1 (2.70%) were from both pleural fluid and ear swab samples. All the 4 isolates were co...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...ovince and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu di...
Hamida Sarwar1, Kousar Perveen1,Rida Mazhar2, Muhammad Afzal1, Syed Amir Gilani3
...ter clinics and COVID-19 isolation wards were eligible. After obtaining informed consent and assuring confidentiality, statistical data, and mental health measurements from 73. The instrument used for this study was focused on depression, anxiety, distress, and insomnia using the Chinese genre of authorized measurement tools. Removed reference statistical evaluation was performed by using SPSS version 2.6. The data was analyzed to assess the severity of sympto...

Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz

Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
... study was conducted for isolation  and molecular identification of new strains of C. perfringens Type D for effective diagnosis, treatment and vaccination. A total of 100 fecal samples  were collected aseptically from four different zones of district Peshawar during the period of February, 2019 to April, 2019. C. perfringens  Type D was confirmed through bacterial culturing, Gram staining, biochemical tests and polymerase chain reaction (PCR) ....
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...n technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were identified from Jallo Wildlife Park. Eimeria (7500) and Ascaris (1100) were the most abundant. Murree Wildlife park had only two species i.e. Eimeria (3850) and Strongyloides (350). In Bahawalanagar W...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...o, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on STO feeder cells with SSCs culture medium (SSCM), which is serum-free. SSCs began to proliferate on the second day and quickly formed grape-like clusters t...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...the host resulted in the isolation of a collection of mutants with increased virulence. One mutant, designated, MlUV-40b showed 3.3-fold increase in virulence as compared to the wild type parent, with an LC50 = 0.3 ×105 conidia/ml versus 1.1 × 105 conidia/ml, respectively and LT50
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...’ mastitic milk by isolation on Lowenstein-Jensen medium (LJ medium), Ziehl-Neelsen staining; identification by different biochemical tests and polymerase chain reaction (PCR) at 500bp diagnostic for M. bovis, as well as (2) to determine the therapeutic efficacy of the various antimicrobials (9 types) on the isolated M. bovis by using different antimicrobial sensitivity plate method. A total number of 100 samples of mastitic milk from nativ...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...rovince, Egypt, also for isolation and identification of the local circulating BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections which leads to serious complication which may be fatal in young calves up to 3 months of age. A total of 20 fecal samples were collected in the winter season of 2018 and 2019, all samples were serologically screening by antigen-capture ...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
...ective of this study was isolation and determination of changes caused by Clostridium perfringens type D from sheep. For this purpose, a total of 272 fecal samples were collected from sheep and lambs from four different zones of district Peshawar. Isolates were morphologically, biochemically and molecularly confirmed through Gram staining, gelatin liquefaction test (GLT) and polymerase chain reaction (PCR), respectively. Out of total, 85 (31.3%) were po...

Samra Aftab1, Saleem Ullah1 and Farida Anjum2*

...e data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CFU × 104g-1) followed by Penicillium sp. and Trichoderma sp. (7 CFU × 104g-1 and 5 CFU × 104g-1), while the lowest colonies were noted for Candida ablican and Saccharomyces cerevisiae. The screening test of these microbes for cellulose degradation showed higher degradation for Trichoderma sp. (6 % and 8 %) followed by Penicillium sp. (4 % and 4.5 %) in both of t...

 Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*

...ads to the detection and isolation of this virus from
Egypt. Injection of filtrate induced SGH symptom in 60% injected flies.
The salivary gland symptom was similar to the previously reported
symptoms of the MdSGHV. Examination of the SGH suspension with
Electron microscopy revealed the presence of rod-shape virus particles
with 500-700 nm in length and 50-70 nm in diamete...

Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1

...to design a protocol for isolation
trials and characterization of equine arteritis virus from field specimens. A total of
548 samples were collected from governmental and private studs of Arabian horses
and also foreign horse breeds, including 540 serum samples, among these, 4 EDTAblood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum samples. 130 samples were clearly stro...

El-Habbaa, A.S.

...ected
tovirusisolation on embryonated chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...iseased calves for virus isolation in embryonated chicken eggs for three passages followed by ten successive passages in Madin Darby Bovine Kidney (MDBK) and identification of isolated virus was done by indirect immunofluorescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The results confirmed infection of calves with lumpy skin disease virus (...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...g ELISA test. Successful isolation of the rotavirus on MDBK cell line from two samples from the positive ELISA samples. The beginning of characteristic cytopathic effects (CPE) were observed from the 3rd passages as clumping, rounding of infected cells with syncytia and leaving empty vacuole space in MDBK cell line. The detected rotavirus with tissue culturing was identified by FAT with apple green fluorescence in the cytoplasm of infected cells. Viral RNA was...

Yanni, M. I1; Hanaa A. Ahmed2; Lamyaa, A. Ahmed1; Hanan, A. Fahmy3 and Ibrahim, E.M.4 Aggour, M. G.3

...t antibody technique and isolation on tissue culture using BHK cells. Direct confirmation was performed by Real time RT- PCR and gene sequencing was performed directly from brain samples. Phylogenetic analysis including sequences from all previous Egyptian isolates and neighboring countries isolates was conducted. The % of nucleotide identities of the Egyptian recent isolate (Egy/cairo /2014) with three previous Egyptian isolates were 98.4-98 and 98.4% respect...

Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3

El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.

...ent study, we report the isolation of bovine ephemeral fever virus (BEFV) field strains from clinically infected cattle and buffaloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutral...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...xtraction method for the isolation of
high quality nucleic acid (DNA and RNA) to detect all graft
transmissible pathogens of citrus including prokaryotic pathogens,
DNA and RNA viruses and viroid provides a new approach to virus
and virus like detection and identification. The method provides a
pure preparation of un-degraded RNA and DNA in high yield and
can be completed within 2 h. It is...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

... revealed that the virus isolation is a gold standard technique, as well, the immunofluorescence technique. RT-PCR proved to be a rapid, sensitive and specific tool for bovine ephemeral fever disease diagnosis.

...

Safaa M. Mohammed*, Shahira A. Abdelwahab**,Fetaih,H⃰ ⃰ ,Neveen,R⃰ ,Abdel satar ,A⃰ and Mohamed S. Elshahidy**

Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1

...current study focuses on isolation and molecular characterization of EHV-1 of the local isolate Egypt/VSVRI/Zahraa/2014 using the glycoprotein D of EVH-1 due to its role in virus infectivity and its function in entry of virus into cells and is considered as one of the most potent inducers of virus-neutralizing antibody among the spectrum of EHV-1 proteins based on sequence analysis and multiple alignment revealed single nucleotide substitution at the base pair...

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

...s study was designed for isolation and identification of LSD virus on CAM and MDBK cell culture and identification of isolated virus by FAT and PCR. Methods: A total 23 skin biopsies were used for virus isolation on chorio-allantoic membrane (CAM) of embryonated chicken eggs SPF at 9-11 day which showed pock lesions and CPE on MDBK cell culture. Isolated virus was identified by indirect fluorescent antibody technique (IFAT) ...

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

...ed using classical virus isolation (VI) and titration methods
in (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration of the HPAI virus for infection of SPF chicken. The tracheal swabs were collected
from diseased chicken for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of origin...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...duction worldwide. Virus isolation is the golden standard for IBV diagnosis so the
aim of this study was comparing the different biological systems for isolation of infectious bronchitis
virus.
Methods: The Egyptian variant 2 IBV strain representative to the current circulating strain in Egypt
was isolated in specific pathogen free (SPF) embryonated chicken eggs ECE), chicken e...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...nt study was applied for isolation and molecular identification of Sheep pox
virus from clinically affected sheep in El Menofiya governorate, Egypt during 2016. Additionally, the
isolated agent was identified using electron microscopy (EM).
Methods: Thirty five skin lesions of nodular and crusted scab samples were collected from clinically
infected SPV sheep and were prepared and isolated on chorioallantoic membr...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...CR and
virus isolation revealed that all chicken groups vaccinated at 1 and 5 days of age demonstrated 100%
shedding at 3, 5, 7 and 10 days post challenge. However, groups 3 and 6 which were vaccinated at 10
days of age revealed difference in shedding pattern where group 3 (vaccinated with local prepared
vaccine) showed 100 shedding by rRT-PCR and 100%, 60% and 60 % of the chickens in tracheal swabs
a...

Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid

...iv>RT-PCR testing and re-isolation of ARV from ECEs of a breeder flock of a history of uneven growth
and/or arthritis revealed ARV infection in six (6/60) examined ECEs.
Conclusion: These results indicated the incrimination of ARV infection in some arthritis infections
beside the low food conversion rate of chickens within Sharkia Province, Egypt. Moreover, the
obtained data highlight the importance of vertical t...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...-PCR (rRT-PCR) and virus isolation and titration in SPF ECE.
Results: Results of HI demonstrated significant difference between groups in relation to age of vaccination,
where the groups vaccinated at 10 days of age were significantly higher compared to others with maximum
titers at 4 weeks post vaccination. The protection % post challenge revealed 0, 20, 86 % and 0, 20 and 86 %
in groups 1, 2, 3 and groups 4, 5,...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
... designed for detection, isolation, identification and characterization
of Avian influenza viruses (AIV) circulating among poultry in 2016 to determine its suitability to be
used in vaccine quality control in Egypt.
Methods: The newly isolated virus was identified and subtyped antigenically by serological test as
Haemagglutination inhibition (HI) using standard AI antisera for H5 antigen and genetically by RTPCR<...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

...against CaPVs. The virus isolation,
identification, and vaccine or other diagnostic preparations mainly depend on the proper culture used.
Objective: Our aim was to study the growth kinetic of recent LSDV isolate to determine the culture
properties of recent LSDV isolated from skin nodule that could be useful in the attempts to develop
more effective LSDV vaccine and diagnostic purposes.
Methods: In o...

Samah A. Mokbel, Eman A. Ahmed, Hanan F. El-Kammar, Ahmed A. Kheder

...ibodies against CMV, and isolation from local lesions on
Chenopodium amaranticolor as a local lesion host. Eleven plant species belonging to four families
were used to confirm the presence of CMV in the inoculum. Detection of the coat protein gene of
CMV in infected leaves has been done by reverse transcriptase-polymerase chain reaction (RT-PCR),
and the appearance of 678 bp bands confirmed the expected size of s...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
.../i>contamination; isolation and identification of Salmonella serovars was done using the standard procedure, while antimicrobial profile was illuminated using the disk diffusion method. The results indicated that 21.34% beef samples exhibited the Salmonella contamination. The statistical analysis exhibited a significantly higher (p< 0...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...ed using bacteriological isolation and serological identification. PCR amplification of the ESBL and carbapenemase-encoding genes was performed. The bacteriological examination of the samples showed that 6 Salmonella strains [S. Typhimurium (3), S. enteritidis (2) and S. Infantis (1)] were isolated from chicken meat and eggshell surface samples. In human, it was found that 6 salmonella strains (S. Typhimurium, S. Enteritidis,...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
...apy due to their ease of isolation, proliferation, anti-apoptotic and proangiogenic properties. MSCs have the ability to fuse with somatic cells in vivo and depending upon the microenvironment, the fusion products may perform tissue-specific function. Stem cell factor (SCF) is one of the important cytokine for cardiomyocyte survival following cardiac injury. The main objective of the present study is to determine the fusion potential of SCF transfected ...
Manar M. Farouk1*, Amal El-Molla1, Fayez A. Salib1 and Yousef A. Soliman2
...nce included, absence of isolation of newly- purchased animals and isolation pen for sick animals, over the counter use of antimicrobials, lack of disinfection of feeding utensils and water troughs, and presence of rodents in feed storage area. Enterotoxin (stn) gene was detected in all recovered salmonellae, and the phylogenic analysis of stn gene of the selected strains and the retrieved sequences from GenBan...

Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi

...ar level. Bacteria after isolation were labelled as EPF1 while by molecular confirmation this bacterial strain was named as Exigoubacterium auranticum. Optimum bacterial growth response was recorded at pH 7.0, temperature 37oC, LB media and shaking 160 rpm conditions. Cell surface characteristics of E. auranticum (EPF1) was determined in terms of bacterial cell surface hydrophobicity and auto-aggregation assay. Highest percentage hydrophobicity values of the s...

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

...arms used quarantine and isolation shed (6%) for the disease affected cows. Among the risk factors identified; type of farm, floor type, quarantine facility, isolation shed used, adequate drainage facility were statistically significant (P ≤ 0.05) to causing both SCM and udder lesions. Predominant causal agents isolated were Staphylococcus sp 186 (70.4%), Streptococcus sp 146 (55.3%), Bacillus sp 62 (23.5%) and E. coli 20...

Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*

...om other sites also. The isolation of Pseudomonas aeruginosaphages from residential wastewaters is a promising molecular tool in combating the global antimicrobial resistance threat.

...

M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2* 

...angle) were subjected to isolation and identification of Pasteurella species by conventional phenotypic method and genotypically by polymerase chain reaction (PCR). At the level of biochemical characterization, the incidence of Pasteurella multocida was 40.4%, and Mannheimia haemolytica was 7.1%. Using KMT1 gene for identification of the isolates for P. multocida and SSE gene for identification of the isolates for M. haemolytica, the results revealed that six ...

Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2 

...bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus isolates were the most identified (42%), followed by Trichosporon (10.5%), Penicillium (10.5%), Fusarium (5%), Candida (1%) and non-identified isolates (26%). Furthermore, the obtained 8 Aspergillus isolates were further morphologically identified into A. fumigatus which was most frequent species iden...

Lifang Zhang, Xiuyue Zhang and Bisong Yue*

...result from geographical isolation and barriers to gene flow. These results would provide the basis of further study on genetic diversity and structure.

...

Hams M.A. Mohamed1, Mona A. El-Zamkan2* 

... many infections and its isolation from raw milk represents a potential risk for human health. According to our best efforts of research, it is the first time to isolate S. thoraltensis from raw milk in Egypt.

Keywords | Raw milk, Streptococcus thoraltensis, Antimicrobial resistance, Biofilm production, Proteolytic activity 

...

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...0% respectively. Initial isolation revealed 14.2% and delayed isolation procedures revealed a prevalence of up to 21.42%. The bacteriological analysis was carried out using conventional cultural and molecular means (cyclic polymerase chain reaction; cPCR) targeting the invA gene. The isolated Salmonella culture revealed higher resistance incidence up to 100% against amoxicillin-clavulanic acid (AMC; 30 μg), ampicillin (AM...

Imtiaz Ali Shah1*, Maleeha Anwar1, Rafiullah2, Inamullah Wazir1, Muhammad Hasnain Riaz1, Abdur Raziq1, Muhammad Ijaz Ali1, Faizul Hassan1, Khalid Khan3, Yasin Ahmad4, Irshad Ahmad5, Muhammad Ibrahim Rashid5, Muhammad Tariq Zeb1* 

...uidelines for Salmonella isolation. A total of twenty-two (22) eggs were positive for Salmonella species. The antibiotic susceptibility testing revealed that Salmonella isolates were resistant to ampicillin, amoxicillin/clavulanic acid, cefotaxime, and kanamycin while isolates were susceptible to chloramphenicol, gentamicin, kanamycin and streptomycin. These positive samples were further investigated and confirmed through PCR by targeting serovars specific gen...

Ejaz Ali and Nageen Hussain*

...Gilgit-Baltistan for DNA isolation and PCR was done by using a specific primer while sequencing was done by Sanger sequencing. Mutations were detected by Mutation Surveyor and BLAST. Protein structures of both control and mutated samples were constructed by PHYRE2 and visualized by the software PyMOL. The detected mutations were 380G>A (R127H), 457G>A (V153I), 36T>C (G12G), 496C>T (L166L), 650 delA and 79G>A (V27I). This study revealed that the ...

Stephen Chijioke Emencheta1, Anthony Amaechi Attama2, Ezinwanne Nneoma Ezeibe1, Adaora Angela Agubata1 and Ebele Benedette Onuigbo1*

... coli was confirmed. The isolation of Escherichia coli phages from residential and farm wastewaters is a promising molecular tool for E. coli tracking in the environment.

...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

...%) in the group (G2) - Reisolation of B. cereus in G2 was 83.3% and 91.6%, while in G3, it was 16.6% and 8.3% on the first and second week, respectively. The results of selected blood parameters and enzymes proved that B. cereus infection exhibit high levels of AST, ALT, ALP, and creatinine with reduced total protein, albumin, globulin, and albumin: globulin ratio. While in the treated group, reversible changes occurred.

Keywords | B. cereus, Enterotox...

Bridget Adah1*, Clara Kwanashie2, Haruna Kazeem2 and Samuel Mailafia1

...ptospira organisms and % isolation from blood sample was 7 (3.5%), while 2 (1%) came from urine samples. Our results demonstrated that the total prevalence of Leptospira organisms in pigs in these areas was 4.5%. Considering the economic and zoonotic significance of this organism, we recommend that proper handling of pork and associated by products, as well as occupationally predisposed personnel should be of great importance especially pig farmers and veterin...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
...t study revealed overall isolation rates of Aeromonas spp. at 48.33%, and 21.66% in the examined crustacean samples collected from Egypt and Saudi Arabia, respectively. Crab samples had the highest prevalence rates, followed by lobster, and shrimp in samples collected from Egypt and Saudi Arabia, respectively. Four Aeromonas spp. were identified, namely A. hydrophila, A. sobria, A. caviae and A.veronii. The recovered isolates of A. hydrophila harbored coding g...
Chinar Mustafa Mohammed1*, Thamer M. Bashir1, Omar A.M. Al-Habib2, Farhad Ramadhan1, Mohammed Bassam1
...ns: Cage control, social isolation in standard rat housing for 14 days, and pharmacokinetics effect of intravenous administration of different doses of taurine on arrhythmia induced by social isolation in vivo after. The ECG signals and parameters were recorded and analyzed with the aid of Bio Amp of ADInstruments data acquisition system and LabChart software. The results showed infusion of Taurine 1mg/kg/hr, 2.5mg/kg/hr and...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

...amples were examined for isolation of E. coli and for colistin resistance gene. Eighty-three selected samples (chicken breast) were subjected to detection of colistin residues by HPLC (High-performance liquid chromatography). In this research, we used polymerase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutations and relationships. The percentage of isolated E. coli was 25.5%. A...

Iram Liaqat1*, Umaima Bibi2, Muhammad Arshad3 and Najma Arshad2*

...een extended further for isolation of potential bioactive components with natural and inherent antimicrobial and antioxidant properties.

...

Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3

...ted of three stages: (1) isolation, identification of LAB obtain from whey, a by-product of cheese production, morphology, physiology, and biochemistry; (2) in vitro probiotic characteristics testing, including survival at pH 3, resistance to 0.3% bile salt media, and antimicrobial activity against Escherichia coli: O157, Pseudomonas, Listeria inokua, and Klebsiella pneumonia; and (3) Molecular identification of LAB by analysis of the base sequence of the 16S ...
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...r of 2019. RT-PCR, viral isolation, sequencing, and phylogeny revealed that the primary causative agent was HoBi-like Pestivirus. Our HoBi-like Pestivirus strain, Egypt-020-1ncp, BVDV-Egypt-020-3ncp and BVDV-Egypt-020-2ncp, shared 99.6% homology with BVD3- B1-AU, 99.2% with BVD3- B5-3-MX and BVD3-G2-BR, 98% with BVD3- Hobi-like virus SA-2016-04 and 95% with BVD3- Italy-68-13ncp isolated in Australia, Mexico, Brazil, South America, and Italy. Multiple area of m...

Veronica Ngozi Emenuga1, Clara Idara Eleazar2*, Seto Tunrayo Aladenika1  

...l contamination. Rate of isolation from cattle, bedding and the milk was not significantly (P= 0.365) different. The distributions of various species within the bedding was significantly different (P= 0.001). Trichophyton mentagrophytes had the highest rate in the beddings (22%) followed by T. verrucosum (16%), while T. verrucosum had highest occurrence rate (24.2%) in cattle samples followed by the T. mentagrophytes (21.2%). Candida albicans was the...

Zohair S. Mulla1*, Mostafa M. Abdelhafeez2 

...of E. coli. In addition, isolation, and identification of shiga toxin producing E. coli were further conducted. An experimental trial was done for improvement of the bacteriological status of the ground beef using diluted acetic acid, citric acid, and their mixture containing equal volumes of both acetic and citric acids at 0.5%, 1%, and 2%. The achieved results in the current study declared high microbial counts for the tested meat products, particularly for ...

Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2

...egative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. The virus glycoprotein B (gB) fragment (580pb) was amplified in selected isolates using the nested PCR (n-PCR), sanger sequencing of gB from three isolates with phylogenetic analysis reveals the full identity between the Egyptian isolates from the outbreaks and other EHV-4 strains available in databases proving wide distance with other ...

Gawhara Ahmed-Abdelmonem1*, Zeinab Aboezz2, Ahmed Habashi1, Saad Sharawi2 

... be FMDV positive. Virus isolation was carried out on ten of the FMDV positive samples that were selected according to their cycle threshold (Ct) values. Moreover, the serotype A amplicons were subjected to sequencing of the VP1 gene. Through phylogenic analysis of three FMDV serotype A isolates, it has been deduced that two isolates were clustered to Asian Iran-05 topotype with close similarities (99.42-99.57%) to the local vaccine strain, while the other one...

Umar Farooq Gohar, Attia Majeed, Bushra Muneer* and Hamid Mukhtar

...e present study, for the isolation of podophyllotoxin producing endophytic fungi 30 plant (Podophyllum hexandrum) samples were collected from different localities of Pakistan. About 261 fungal strains isolated, among them 22 strains had the ability to produce podophyllotoxin. Maximum podophyllotoxin production (88.14µg/ml) was shown by fungal strain P-18, which was later identified as Penicillium sp. through 18S rRNA gene sequencing. Optimum growth condi...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...ication cost, difficult isolation of causative bacterial agents, weight loss, reduced fertility, and hatchability, so this study aimed to determine the bacterial causative agents using multiplex PCR as an accurate rapid diagnostic technique. Exudates of swollen infra-orbital sinus were aspirated and aseptically collected from affected turkeys of seventeen flocks and examined by multiplex PCR against Escherichia coli (E. coli), Mycoplasma gallisepticum (M. gal...

Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1  

...lusion, although the low isolation rate of confirmed MRSA in Ismailia governorate, the three selected MRSA strains invigorate the possibility of transmission among different sources of food, animals, and human, which causes nosocomial infections.

Keywords | Ica-MRSA, PCR, Virulence factors, Resistant factors, Phylogenomics. 

...

Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1* 

...d on different media for isolation of bacterial species. The isolated species were identified by different biochemical tests. The results showed that the contamination of bacterial organisms in both raw and cooked meat was highest in beef followed by mutton and fish respectively (p < 0.05). In raw meat, bacterial species recorded were Escherichia coli (45%, 30% and 25%), Salmonella enteritidis (20%, 17.5% and 15%), Staphylococcus aureus (30%, 25% and 25%), ...

Shabana Ehsan1*, Aneela Riaz2, Muhammad Amjad Qureshi1, Abid Ali1, Ifra Saleem3, Muhammad Aftab3, Khalid Mehmood4, Fakhar Mujeeb1, Muhammad Asif Ali1, Hina Javed1, Fraza Ijaz1, Anwar-ul-Haq5, Khaliq-ur-Rehman3 and M. Usman Saleem

Ahmad Nadeem1,2 and Rubina Arshad1,2,*

...tive media were used for isolation of LAB from plant cuttings. A total of seven isolates were isolated on the basis of colony morphology on De Man Rogosa Sharpe (MRS) agar plates, Gram staining, biochemical tests and molecular characterization. The co-culturing of LAB isolates with fungal cultures (in vitro) showed that among seven LAB isolates, only three possessed antifungal activity. One promising wild type isolate (LPA6) was subjected to gamma irradiation ...

Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana  

...ene, Pesisir cattle, DNA isolation, single nucleotide polymorphism 

...

Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1* 

...n in CLs homogenate, RNA isolation and gene expression. Blood samples were obtained for P4 serum levels estimation. Results showed a significant rise of TNFα mRNA during late stage of CL and increase the expression of CASP3, FASLG and AGTR2 mRNAs during mid-stage of CL. Furthermore, NOS2 mRNA was raised during early stage of CL. Surprisingly, NO in CL homogenate was raised through early and late stages of CL. Additionally, P4 increased in mid-stage of CL...

Farah Naz1*, Asad Umair2 and Ghazala Noureen3

...c, like early detection, isolation of confirmed cases, and social distancing, were build and implemented on the assumption that COVID-19 is simply a health emergency that demands solutions from the life science alone. They failed to take into account structural inequalities present in societies that affect the ability of an individual to cope with this medical condition and follow the recommended strategies. As Geoffrey Rose (1992) concluded his seminal monogr...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*

Sofiane Tamendjari1,2*, Farida Bouzebda Afri1,2, Lina Chaib1, Hebib Aggad3, Zoubir Bouzebda1,2 

...used for enumeration and isolation of strains, free coagulase and thermonuclease tests were performed as well as other biochemical tests. Antibiotic susceptibility testing was performed to evaluate the resistance of S. aureus to antibiotics. Finally, statistical processing was performed using the STATISTICA 7 software (Statsoft, France). The percentage of samples contaminated with S. aureus was 32.22%, and the average load of S. aureus contaminating sheep, chi...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...aled successful M. bovis isolation. Using ELISA on serum samples collected prior to skin testing from the positive tuberculin reactors, only 26 cases (37.7%) were positive. The IQRT Anigen lateral flow kit showed only 21 positive cases, 43.8% of 48 bacteriologically identified cases, and 30.44% out of the 69 tuberculin-positive cases. The Ubio quick VET kit has detected zero% of bovine tuberculosis-positive cattle. Using bovine tuberculosis rapid kits alone ma...

Alsaied Alnaimy Habeeb*, Amaal M. Kamal, Mostafa Abbas A. Atta, Ahmed K. Sharaf  

... of separation while the isolation approach raises the level of the hormone estradiol, which improves the rate of conception.

Keywords | Behavioral parameters, Conception rate, Milk yield, Rabbits, Separation.  

...

Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7* 

...utes. The lack of proper isolation and diagnostic methods as well as improper use of antimicrobial therapy causes the treatment failure. Different types of antibiotics are used to treat it and different research use antibiotics of aminoglycoside group. For preventing the flock from infection separates the infected birds from healthy ones. Different treatments show average growth and good performance with the recovery of clinical signs, histopathological change...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...on of leaf was taken for isolation of bacteria. Lesions were cut into parts and streak bacteria by the help of inoculation loop grown in nutrient medium. Pure bacterial culture of Xanthomonas axonopodis were used for detection by standard PCR. Xanthomonas axonopodis was diagnosed by amplification of 16S rDNA. A fragment of ~1.4 kb was amplified and cloned for sequencing Out of three markers used (K1F-CIT/ K1R-CIT, K2F-CIT/ K2R-CIT, K3F-CUT/ K3R-CIY) K3F-CUT/ K...
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...d MacConkey agar for the isolation of Enterobacteriaceae. Different identification tests have been carried out by using various selective and differential media (MacConkey agar, Eosine & Methylene Blue agar) for isolation and identification of microorganisms.Water samples showed the presence of faecal contamination; especially coliform bacteria which is a risk for the health of local community. Salmonella spp. was detect...

A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe.

...

A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1

Saiwa N. Zein
... is the first report for isolation of Tobacco rattle virus Tohrarirus (T R V) from D.Kaki under the Egyptian conditions.
...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...ilk samples revealed the isolation of the following pathogens from clinical and subclinical mastitis cases, respectively; E. coli (29.41% and 29.2%), S. aureus (19.12% and 25%), Str. agalactiae (16.2% and 16.6%), Str. dysgalactiae (14.7% and 20%), Str. uberis (7.3% and 2.5%), K. pneumoniae (4.4% and 3.3%), Str. fecalis (4.4% and 0%), Str. pyogenes (2.9% and 0%) and Pseudomonas aeruginosa (1.5% and 3.3%). Among the recovered E. coli serotypes, the serotype O111...

Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2 

... scheme of accumulation, isolation, isolation and identification according to DSTU ISO 10272-1:2007. As a result, 33 isolates of Campylobacter spp. were isolated, which is 1.6% of the total number of the researched samples. The most common phenotypes of antimicrobial resistance of the selected isolates were: Cip / Tet / Ery - 14 isolates from poultry, which accounted for 42.42%.  Tet / Ery - 4 isolates from poultry...

Sumbal Nazir1*, Farkhanda Manzoor2, Ishrat Perveen3, Anum Rana2, Irum Naureen1, Abad Ali Nadeem3 and Hafiza Najma Naeem2 

...nies was followed by RNA isolation. The RNAs were quantified on spectrophotometer. Results have shown the occurrence of fungus and/or pathogen infestation which was caused by microsporidium Nosema apis with 277bp. It has been observed that the honeybee colonies were badly affected due to Nosemosis disease which was caused by microsporidian fungus Nosema apis. This microsporidian fungus causes dysentery in the honeybees, may impede the survival rate of bees, an...

Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1 

...ical tests, and bacteria isolation kits were used for the isolation and identification of bacteria. The genus Vibrio, Aeromonas and Pseudomonas were identified in different components of the larval production line in the hatchery. V. mimicus, V. parahaemolyticus, V. harveyi and V. alginolyticus were the major species identified from the Vibrio genus. The average concentrations of Vibrio spp. in brood, brood’s tank wate...

Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1 

... the broilers. Bacterial isolation following liver translocation revealed the existence of a number of bacterial entities. Most of them were foodborne pathogens, in fact, all the groups displayed close levels of infection by Escherichia coli, Staphylococcus aureus, Salmonella spp, and Staphylococcus spp; And Yersinia enterocolitica was also detected on one of the farms. Broiler performance can be affected to varying degrees by biosecurity levels on farms. High...

Aneela Ahmed Tagar1*, Mahvish J. Channa2, Kanwal Ahmed Tagar3 and Kaka Kainat4

Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

...gh significant) of yeast isolation of (67.74%) with 42 isolates which were detected by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 10...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...mples were processed for isolation of bacterial organisms using conventional culture method and isolates were tested for antimicrobial sensitivity according to CLSI method. According to results, the prevalence of bacterial organisms was high (p < 0.05) in CC than CM and LR. E.coli were the most common organism in market and pond fish samples with occurrence of 86.6% and 66.6% respectively. Next most prevalent pathogens were Salmonella spp. (78.3% & 58.3...

Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2* 

...ected for the purpose of isolation and diagnosis resist.E. coli 0157:H7 two hundred urine samples were collected from adult women patients suffering from UTIs during a period from February 2022 to July 2022 in Diwaniyah city Iraq . All urine samples were incubated aerobically at 37°C for 24-48 hrs on blood agar, MacConkey agar as well as biochemical tests identified special media and the isolates. The isolates were confirmed and diagnosed by Vitek assay. ...
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*
...f CQ. Further studies on isolation and functional characterization of active compounds of signaling pathways that mediate osteoclastogenesis will contribute further insights on the anti-osteoporotic therapeutic activity of CQ.

...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...ogical applications. The isolation and purification cost of the enzyme is highest, so there is a need to develop cost-effective purification methods. This research work aims to purify BRM by using magnetic nanoparticles. After purification, BRM enzyme was characterized and utilized in the detergent industry due to its stability at a wide range of pH and temperature; results showed that stem BRM has the highest enzyme activity at 23.5 U/ml as compared to crown ...

Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3 

...as planned to assess the isolation, identification and antibiotic resistance profiling of Salmonella in chicken and ducks. A total of 125 feces samples (55 broilers, 45 layers and 25 ducks) were collected in district Hyderabad. Isolation was done according to ISO 6579 standard method. Identification of isolated bacteria was done using the Salmonella specific biochemical tests. Antimicrobial resistance patterns were determine...

Putu Henrywaesa Sudipa1*, Hapsari Mahatmi1, Ketut Tono Pasek Gelgel1, I Gusti Ketut Suarjana1, I Nengah Kerta Besung1, Romy Muhammad Dary Mufa2 , I Wayan Sukernayasa3 

... in Bali, Indonesia. For isolation, vaginal swabs were cultured on Sabouraud’s dextrose agar (SDA) medium and identification was performed macroscopically by observing colony growth and microscopically using the tape smear method stained with Methylene Blue stain. The results showed that 32 fungi belonging to seven genera were isolated from 22 Bali cows. The prevalence rates of Aspergillus, Fusarium, Cladosporium, Curvularia, and Candida, Mucor, and Pen...

Raed Hussein Salih Rabee1, Yahya Sabah Abdulameer1, Walla Farhan Obed1, Noor R Abady1, Adnan Mansour Jasim1*, Firas Hussein Albawi1, Mohammed Jasim Jawad2, Ahmed Samir Abukhomra3

...g from diarrhea, and its isolation was confirmed by culturing it on MacConkey agar medium. In this study, a total of one hundred chicken chicks were reared, and on the tenth day of the experiment, they were divided into five equal groups:NCG: This group represented the control and was given normal drinking water. T1: The chickens in this group were orally inoculated with E. coli at a dose of 0.5 ml containing 6x108 CFU/ml of E. coli. T2: The chickens in this g...

Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3

...uiring less time for SVF isolation and gives better cell yield per gram. SVF preparation process has advantages of minimum cell contamination and processing time. Hence, it is a cost effective and alternate procedure for developing countries like Pakistan.

...

Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.

...signed for detection and isolation of Avian Influenza Viruses (AIV) circulating among poultry since their first detection in 2006. Tracheal and cloacal swabs were taken from the freshly dead birds. Till now (2010) the tested swab samples were inoculated into the allantoic cavity of 911-day-old SPF embryonated chicken eggs (ECE) for virus isolation. The allantoic fluids (AF) of the inoculated ECE were examined for detection o...

El-Dougdoug, Kh.A.; Rezk , A.A.; Dawoud, Rehab A. and Sofy, A.R.

...is constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt

...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K  and  Mona M.Aly 
 
.... This study records the isolation and characterization of H5Nl virus from different species of the zoo birds and also studies the immune states of the vaccinated birds after application of H5Nl vaccine for the first time one month after the introduction of early outbreaks of 2006. Viral diagnosis was based on direct detection of viral RNA by real time PCR. Two positive cases from turkey and Grocer duck were processed for viral isolati...

Ahmed Abd El Samie Hassan Ali

...21 post inoculations for isolation and detection of its antigens on MDBK cells using outgrowth ELISA and immunofluorescence as well as measurement of BVDV antibodies by serum neutralization test. Virus antigen detection was reported on days 5 & 7 while the antibodies were initially detected on day.

...

JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.

... sheep and goats for the isolation of PI-3 virus. The samples were taken from diseased animal at seven different Governorates Kaffer el sheikh, Alexandria, EL Behaira, Port Said, Demiatta El-Qalubia and Giza through the winter seasons of years 1999 to 2003. The virus was successfully isolated from three ovine samples after three successive passages on MDBK•cells. The isolated viruses were then titerated and identified using different biological, serologic...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...nodes and lung) for BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field case...

Abou zead, A.A., Ali, A.A.H.*and Abd El Hkeem M.M.

...inogen. Trials for virus isolation on the CAM of chicken embryos revealed the isolation of 3 IBD viruses from diseased quails. Isolated IBDV viruses '.vere examined by electron microscopy. The disease was reproduced in quails to record the course, the clinical findings, post mortem and histopathological changes. Also seroscreening of serum samples by AGPT were done. The present findings focus the attention to the importance ...

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1

... (RT-PCR). Whereas virus isolation followed by immunostaining procedure in cell culture confirmed only 6/9 BVDVs that were cytopathogenic. Direct sequencing of the RT-PCR amplicons revealed an extreme nucleotide sequence homology (≥ 98%) among all tested Egyptian isolates versus the local vaccine strain. sequence alignments showed variable identity (74% - 93%) of the Egyptian vaccinal strain versus reference laboratory and vaccinal strains of BVDV. Clusteri...

Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2

...ent study, we report the isolation of BVDV field strains from apparently health buffaloes. One hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez governorates in Egypt during the year 1999. Screening of samples by GAPT using standard known anti-BVDV antisera revealed that three positive samples for the presence of BVDV antigen. In a trial for the

Sulake Fadhil Al-Zubaidi1*, Ghusoon A.A. Alneamah2, Ali Saleh Mahdi1, Abdulraheem Abduljalil Wali3

...al anesthesia. Bacterial isolation was made on chromogenic agar to identification of bacteria associated with pyometra. In group I after 24 hr. from giving Aglepristone there was vaginal discharge, in some cases, we need to give a second dose of Aglepristone to bitch. In group II, PGF2α was injected after pus came out from the vulva, there was a rapid evacuation of pus from the uterus in comparison with group I with many side effects such as tachycardia,...

Taleeha Roheen1, Shagufta Kamal1*, Shazia Anwer Bukhari1, Ghulam Mustafa1 and Saima Rehman2

Haseeb Zafar1, Asghar Ali Kamboh1*, Zain-Ul- Abideen1, Muhammad Kamran Taj2, Sibtain Ahmad3 , Umbreen Zafar2 

...eg;C for 24 hours. After isolation, organisms were further identified through Gram staining, and different biochemical tests. Among the total samples, 92(61.33%) showed positive growth of E. coli whereas 58(38.6%) samples were negative for E. coli. Source-wise distribution results showed that E. coli was mostly isolated from water samples (88%) followed by cecal samples (80%), air samples (72%), feed samples (48%), bedding samples (40%), and flies samples (40%...

Frennada Lu’fatul Azizah1, Osfar Sjofjan2*, Eko Widodo2

... (Black Soldier Fly) oil isolation process was initiated using the Soxhlet method. Subsequently, 160 broiler chickens were utilized in the research. An experimental design in the form of a completely randomized design with five treatments and four replications was employed, involving eight broiler chickens per treatment per replication. The feed treatments were formulated as follows: T0 (basal feed), T1 (basal feed + 0.5% bacitracin), T2 (basal feed + 0.5% BSF...

Abdul Haseeb1, Yasir Iftikhar1, Muhammad Ahmad Zeshan1*, Safdar Ali2, Rana Binyamin3, Salman Ghuffar4 and Muhammad Usman Ghani5,6

... method was used for the isolation of T. semipenetrans which showed that the population was low in declining orchards as compared to the population densities found in healthy orchards. Kinnow was the most infested by nematode with mean population 1161 while sweet orange was the least infested with 595 nematodes, in all localities. Maximum nematode population density in healthy and declining orchards was recorded in Kot Momin that was 2600 and 946 nematodes per...
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
... study was conducted for isolation, identification, and rapid detection of Avibacterium paragallinarum, the causal agent of Infectious Coryza (IC), from layer chickens in various farms in Pakistan. A total of 46 isolates were obtained from a total of 244 clinical samples from 122 sick and moribund or dead layer chickens showing signs of IC like slight swelling of infra orbital sinuses and nostril mucus membranes. Two sampling sites were chosen, squeezing the n...

Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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