Hu Zenglei1 and Xiufan Liu1, 2*
...ogical manifestations in chicken organs, especially in lymphoid tissues. Genotype VIId of NDV, which is currently endemic in many countries of Asian and Middle East, replicates at a significantly higher level, induces more potent innate antiviral and inflammatory response, and causes more severe damages in lymphoid tissues when compared with virulent viruses of other genotypes. Therefore, severe pathology in immune organs, caused by genotype VIId of NDV, is as...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg
...ian influenza (HPAI); (A/chicken/Egypt/VRLCU67/2011) variant subclade 2.2.1.1 and (A/chicken/Egypt/13VIR3729-4/2013) classic subclade 2.2.1/C that were sodium hypochlorite, calcium hypochlorite (bleaching powder), Virkon®S and Peraclean®. The purpose from using the Egyptian H5N1 viruses in the evaluation was to achieve maximum simulation of Egyptian field reality as the two viruses represent the t...
Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid
Determination of Early Colonizer Urban Blowfly Species Using Small Bait Traps on a University Campus
Halide Nihal Açikgöz1*, Serdal Kenan Köse2 and Ali Açikgöz3
Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A
...to differentiate between chicken infectious anemia (CIA) and infectious bursal disease (IBD) and co-infection of CIA and IBD. Infectious bursal disease was tentatively diagnosed in 5,000, 5 weeks old Hy-Line and ISA Brown pullets in a commercial poultry farm in Jos, Plateau State, Nigeria with mortality of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, trembling and mortality that lasted for 6 days. Postmortem...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...ances in two Anhui local chicken breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production performances including age at firs...
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...(namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently comparing to RSF/1 at all-time points. Further, strain V3 induced significant up-regulation of IL-1b and IL-6 at 24 hours post in...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...orted previously both in chicken and feral birds (natural reservoirs) in Pakistan; however, their molecular characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular analysis of fusion (F) and hemagglutinin (HN) genes of NDV strain isolated from an outbreak in a peacock flock was undertaken. Proteolytic cleavage site of F0 showed a cleavage motif (112RRQKR↓F117), rep...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...ressive disease in young chickens. The first line of defence against the virus is innate immunity whose activation is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of inter...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
...ant neoplasm in domestic chickens with subsequent economic losses to poultry industry. Effected birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked ...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
...e collected from broiler chicken farms in and around Quetta district. Physical properties i.e. moisture and pH were determined by oven drying and pH meter, respectively. Viable fungal count of the feed samples was estimated by using spread plate technique. Identification of fungal isolates was carried out on the basis of cultural and morphological characters by using slide culture technique. While mean moisture percentage was recorded as 5.3% with a sig...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...s codon optimized to the chicken biased codons and subcloned into the pCAGGS plasmid vector under the control of the chicken β-actin promoter. The in vitro expression of recombinant plasmid DNA was performed by transfection of 293T (HEK) cell line. The HA protein was analyzed using SDS-PAGE followed by Western blot and immunofluorescence assays. The pCAGG-optiH9 vaccine efficacy was evaluated by intramuscular immunizati...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...oculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the rate of 0.1ml of EID50 (virus titer 105.50/100μl) on 2nd week of age through eye drops. Group B specific antibody negative (SAN) chicks were challenged with same do...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...ns are being applied for chicken flocks, which have maternal derived antibodies (MDA) for these viruses. To understand the interference of maternal derived immunity impact of maternal derived immunity interference in vaccination regimen in poultry, a total of one hundred day old commercial broiler chicks were vaccinated with LaSota. A group of chicks (n=40) were immunized using baculovirus (H5 and ND) inactivated vaccine via subcutaneous route. Birds (n=20) we...
Muhammad Ayaz1,*, Muhammad Athar Abbas2, Pervez3, Noorulain3, Yasir Amin1, Zubair Ali3, Naila Siddique2 and Khalid Naeem2
...rried out on embryonated chicken eggs. Identification of H protein was undertaken through hem agglutination Inhibition (HI) test while N typing was done using reverse transcriptase polymerase chain reaction (RT-PCR) procedure. Out of 600 examined locations twenty two (22) H9N2 subtypes isolates were recorded. The total prevalence recorded was 3.66%and out of 498 broiler operations yielded 17 (3.4%) positive isolates and 44 golden type nat...
Vladimir Avdalovic1, Marijana Vucinic2, Radmila Resanovic3, Jelena Avdalovic4, Danka Maslic-Strizak5 and Milos Vucicevic3*
...matitis (FPD) in broiler chickens. The study included 300 one-day-old Ross 308 broilers. One group of broilers was reared on chopped wheat straw (the particle length of straw ≥ 10 cm). The second group was kept on newly formulated pelleted wheat straw. The duration of the trial was 42 days. Moisture content in litter materials and FPD occurrence and severity were assessed weekly. First signs of FPD were observed in 21-day old chicke...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HEK (Human Embryonic Kidney) cell line for in vitro expression of the recombinant plasmid DNA. The confirmation of H5 protein expression was performed using SDS-PAGE followed by Western blotting and immunofluorescence assays. The humor...
Alaeldein M. Abudabos1,*, Abdullah H. Alyemni2, Elsayeid O. Swilam1 and Mu’ath Q. Al-Ghadi3
...l coccidiosis in broiler chicken.
...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
... collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 250 cfu from MS and MG respectively in a samples. We have tested pure DNA of other my...
Karim El-Sabrout* and Mohamed H. Khalil
...g light generally during chicken eggs incubation has been shown to affect hatchability, but using light-emitting diode (LED) precisely has not been fully examined. This study aims to evaluate the effect of LED lighting during incubation of Fayoumi eggs on hatchability (period length, dead embryos and percentage) and hatch chick performance (weight, vitality). The experiment was carried out in two groups with total number of 1800 eggs (900 eggs for each group)....
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
... ...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
Dildar Hussain Kalhoro1,2,, Shan Liang1, Muhammad Saleem Kalhoro2, Shoaib Ahmed Pirzado2, Nasir Rajput2, Muhammad Naeem2, Fahmida Parveen2 andYongjie Liu1*
...ted from SPF embryonated chicken eggs. Sequencing analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed 93 to 99% similarity between each other. The CIV isolates shared high similarity (above 98%) to the H3N2 viruses from dogs in China. On the comparison of gene sequence four unique mutations were found in the amino acid of HA (A144T, R158K, D291N, L383F) and NA (T19A, V33L, V82A, S336N). The HA and NA genetic evolution analysis revealed that one ...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...hering Huainan partridge chickens. A total number of 360 fast feathering Huainan partridge hens and 40 roosters were randomly divided into two equal groups. For NM, 4 mating cages were maintained each with 5 roosters and 45 laying hens. The other birds were reared in 3-layer complete ladder cages for AI, for which 180 hens were maintained in 60 cages with 3 hens/cage and 20 roosters in 20 cages. Laying performances, mortality, egg quality characteristics and w...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...isolates from humans and chickens. Our results revealed that linking the epidemiological and sequence data is important to understand the prevalence, transmission, persistence and evolution of the virus, and to monitor the circulating AIV strains and emergence of new AIV subtypes in Egypt. The wide circulation of the 2.2.1.2 subclade, carrying triple mutations (120, 129∆, I151T) associated with increased binding affinity to human receptors, is an alarmin...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...igeons (n=9; 3.6%), desi chicken (n=9; 3.6%), broiler breeder (n=3; 1%) and layer (n=2; 0.8%). Overall sero-prevalence was 53% and average range of the hemagglutination inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) of the samples were positive for viral matrix gene. Sub-typing was performed by specific anti-sera. PCR amplification was done by using N2 specific primers that con...
Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1*
...nteritis (NE) in broiler chickens. The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25...
Mohammed Al Bratty1, Hassan A. Alhazmi1, Somaya Jadoh Ogdi1, Jana Ahmad Otaif1, Abdul Jabbar Al-Rajab2, Mohammad Firoz Alam3 andSadique Akhtar Javed1,*
...ent body parts of Baladi chicken from Jazan region, Saudi Arabia using Inductively Coupled-Plasma Optical Emission Spectrometry (ICP-OES). The concentrations of heavy metals in various chicken parts were recorded to be in the range of nd-0.01, 0.07-0.18, 1.08-3.95, 9.61-119.0, nd-0.16, 0.13-1.10, and 8.63-67.58 mg/kg for Cd, Cr, Cu, Fe, Ni, Pb and Zn respectively. Relatively higher metal concentrations were observed in bone,...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,*
...contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parameter and decrease in MDA contents significantly (P<0.05). SM treatment of TD-afflicted birds prevented lameness and reinstated antioxidant imbalance. SM may be effective for the treatment and control of TD and minimizing the liver...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
...mon food samples such as chicken, beef meat, raw milk, vegetables and salad samples were collected from retail market, the overall contamination of food samples with infectious agents was 48.37%. Campylobacter jejuni was recorded much higher (28.99%) compared to other foodborne pathogens. Food samples were also found positive for Salmonella spp. (19%), E. coli (O157:H7) (8%) and 1.25% for Listeria monocytogenes. 5% tested foo...
Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
...tially expressed gene in chicken sperm storage tubules. In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelia...
Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair4
...d. Twenty one vaccinated chicken flocks suffering from different form of lymphomas, representing different governorates in the Egyptian Delta, were screened for MDV infection. Pathological findings in the visceral tumours and presence of viral DNAs with variable mitotic figures were observed in the examined visceral organs. Eight samples were screened positive for the major MDV-encoded oncogene Meq which was subjected for alignment and phylogenetic analysis. T...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...n and culture methods of chicken amniotic fluid stem cells (CAFSCs) in vitro, characterizing CAFSCs and inducing CAFSCs to differentiate into three layers. Methods and results include that more CAFSCs are gained via thermoelectric method with lower cost. Groping and optimizing culture system abiding by the idea of simulating the chicken body environment, involving adding proportion of chicken<...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ardium syndrome (HPS) in chickens worldwide. Identification of fowl adenovirus (FAdV) serotype is of most importance in epidemiological studies of disease outbreak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties o...
Ali Karabayir1,*, Nazan Arifoğlu2 and Mustafa Öğütcü3
...mance of three different chicken strains, Plymouth Rock, Australorp and Village (local mix-breed village chickens) was evaluated in the present study. Supplementation of disinfectant clearly improved the microbiological quality of chicken drinking water, by reducing the total bacteria, coliform and E.coli counts. On the other hand, disinfectant addition decreased feed and water inta...
Shoukat Ara1, Sheikh Adil2,*and Manzoor Ahmad Khan3
...laying performance layer chicken, a study was conducted on 240 key stone golden layer chicks which were randomly assigned into five groups having three replicates of 16 each. Birds in control (T1) group were fed the basal diet, where as other groups the groundnut cake was replaced with 5 (T2), 10 (T3), 15 (T4) and 20% (T5) Azolla. The results revealed that inclusion of Azolla showed a non-sig...
Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq2
...al changes in free-range chicken (Gallus domesticus) naturally infected with Toxoplasma gondii (T. gondii). Blood samples of chicken (n=71), were collected from Cholistan areas of Bahawalpur (Punjab), Pakistan. Serum samples were tested for toxoplasmosis with a commercial latex agglutination kit (Antec Diagnostic products, UK). The overall prevalence was 26.76%. The significant (P<0.05) higher seroprevalence was observed ...
Tahira Batool1*, Nabila Roohi1, Athar Mahmud2
...ties of indigenous Aseel chicken. A total of 240 day-old chicks, 60 from each of the variety were taken randomly and sub-divided equally into three groups (A, B and C). They were offered three lysine regimens i.e., L1 constituting 1.3% lysine from 0-6th week (1-phased) to group A and L2 containing 1.4-1.2 % lysine where 1.4% lysine was offered from 0-3rd week and 1.2% lysine from 4-6th week (2-phased) to group B, while, L3 lysi...
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...ponse in
broilers chicken against avian influenza virus infection....
broilers chicken against avian influenza virus infection....
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7
... trials in 7-11 days old chicken embryonated eggs. The viral loads were determined through heamagglutination (HA) test. The methanolic extract of each plant was found effective against NDV but in varying order. The active extracts were further used in different concentrations and IC50 of each extract was calculated. The extract of Achyranthes aspera was found most effective with IC50 3.125μg and the extract of Oxystelma escul...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...n but also it can infect chickens and its virulence enhanced upon passaging in chickens causing clinical signs and deaths. In the present study brain samples were collected from twelve pigeon flocks exhibiting signs of nervousness and diarrhea in a live bird market (LVM) in Egypt. All collected samples were positive for NDV and negative for avian influenza virus and infectious bronchitis virus. Seven positive samples were su...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
...in heat stressed broiler chicken. A total of 360 (Hubbard) one-day old broilers were reared under heat stress (38 ± 1°C) and randomly divided into three groups i.e. A, B and C as control, basil seed at 5g/kg and ascorbic acid at 200 mg/kg supplementation, respectively. Improvement of intestinal villus seen in basil supplementary group (P>0.05), and significant increase in the absorption of crude protein (CP), crude fibre (CF), and metaboli...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...ich the output factors [(chicken survival rate (%), egg yield (%)] are optimized at the same time and the appropriate value ranges for the input factors [temperature (oC), humidity (%) and feed per hen (g)] that provide effectiveness ii) Determination of targeted improvement values for the ineffective months to become effective. With this hybrid approach, which is based on the integration of the Data Envelopment Analysis (DEA) and Grey Relational An...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...red with that of broiler chicken kept as standard. A 106.25/ml/bird (chicken lethal dose 50 (CLD50)) was injected to ostriches and broiler (control) and were clinically observed for 7 days. Our results showed that a peak cloacal temperature was recorded on day 3 and day 4 with a 2.3°F and 1.2°F maximum rise in broiler and ostrich, respectively. A decrease in feed and water intake was recorded in...
SiRui Wang1,2, Fekede Regasa Joka1,2, XiaoLong Wang1,2,* and SuYing Bai2,*
... resistance site, in the chicken Mx gene. The third locus in all tested amino acid sequences are Ser and corresponds to the chicken 631 amino acid position, the virus resistance site. Ten haplotypes were found with 60 nucleotide mutations. Phylogenetic analysis by MEGA 7.0 revealed that the evolution of the tested genes is consistent with the evolution of the tested birds. The results of the selection stress test show...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...3 Japanase quails and 22 chickens were analyzed by conventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by d...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...recently been shown that chicken erythrocytes constitutively express toll-like receptors (TLRs), cytokines, and avian β-defensins (AvBDs). Host defense peptides (HDPs) are important effector molecules in innate immunity. In this study, thiram has been used to induce tibial dyschondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored ...
Nursinatrio and Rudy Agung Nugroho*
Kubra Siddique*, Ghaffar Ali, Irfan Ullah, Atta Ullah Shah and Muhammad Fayaz
...rural region followed by chicken, fish and other meat type (Eggs, Shrimps, Prawns). Empirical results show that for all the selected meat type expenditure elasticities are positive suggesting them as normal goods. The expenditure elasticities for mutton and beef are greater than one and are expenditure elastic while chicken, fish and other meat type are expenditure-inelastic. Own-price elasticities for the beef,
Zahin Anjum1*, Farhat Shehzad1, Amina Rahat1, Hamid Ullah Shah2 and Saleem Khan3
...ated meat, such as beef, chicken, mutton and fish, and different grilling techniques were applied to see their effectiveness in lowering the levels of these toxicants. Polyaromatic Hydrocarbons and heavy metals refer to a large group of chemicals that may be present in the environment as pollutants and can enter the food chain and might be carcinogenic to humans. The lead content was found high in fish with the mean value of 3.18mg/kg and the lowest values wer...
Amany Adel, Wesam Mady*, Zienab Mosad, Fatema Amer, Asmaa Shaaban, Dalia Said, Marwa Ali Abdel Maged, Abdel-Satar Arafa, Mohamed Kamal Morsi, Mohamed Khalifa Hassan
...e cases were detected in chicken as the highest prevalence (90%) among all the examined species, Genetically, the genetic sequence for the Hemagglutinin (HA) and Neuraminidase (NA) of circulating Egyptian viruses are belonging to the Middle East G1- like viruses in group B that were closely related to each other and scattered phylogenetically in different subgroups with the presence of variant viruses in the quail during 2015. ...
Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3
...sp;balady and 7 broiler) chicken flocks located in different localities at Sharkia governorate, Egypt. These samples were subjected for direct detection of a 620 bp hypervariable region in the VP2 gene using Reverse transcriptase polymerase chain reaction (RT-PCR). The nucleotide and deduced amino acid sequences for VP2 hypervariable region of selected five IBDV field isolates were determined and compared to well characterized reference and vaccine strains wor...
Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan
...samples were detected in chicken with highest prevalence (90%) for H9N2 infection among all the examined species, followed by Turkey (4%), duck (2.6%) and quail (2.4%), respectively. Genetically, the genetic sequence for the Hemagglutinin (HA) of 38 viruses circulating Egyptian viruses during 2015 and 2016 were belonging to the Middle East G1- like viruses and scattered phylogenetically in different subgroups with the presence of variant viruses in the quail d...
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
Comparison of Yolk Fatty Acids in Market Available Black-bone Chicken Eggs and Ordinary Chicken Eggs
Suhu Duan, Liuan Li*, Zaiqiang Li, Mengran Qin, Zhenzhen Fan, Keyan Zhang, Qian Wang, Zhongmou Zhang, Bailiang Yang
...ially available ordinary chicken eggs and Brand A and Brand B black-bone chicken eggs, thus providing theoretical and practical references for selective consumption of chicken eggs. The results have shown that a total of 18 types of fatty acids are detected in 3 types of chicken egg yolk, including 6 types of saturated fatty acids (SFA), 12 types of unsa...
Chen-Yu Sun, Wen-Chao Liu*, Mei Xiao, Zhi-Hui Zhao and Li-Long An*
...ale, one-d-old Huaixiang chickens with an average initial BW of 44.52 ± 1.07 g were randomly allotted to 4 treatments. Each dietary treatment consisted of 6 replicate cages, with 15 birds per replicate. The 4 dietary treatments were corn-soybean meal-based diets and supplemented with 0, 500, 1000 and 2000 mg/kg betaine, respectively. During d 1-14, with increasing the dietary betaine levels from 0 to 2000 mg/kg, the BWG was improved (quadratic, P<0.0...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
...megaly syndrome (HSS) in chickens. To the best of our knowledge the circulation of aHEV in chicken has not been studied in Pakistan so far. Therefore, in the present study, we aimed to isolate and identify aHEV from layer chickens of Pakistan. The bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki...
S. Jalbani1, N.T. Narejo2,* and P. Khan2
... tubifex larvae, chopped chicken viscera and dead small fish (tilapia) were used as live and prepared feed with control. It was observed that the experimental fish fed with chopped chicken viscera exhibited significantly better growth (129.10 ± 2.90 g weight gain) followed by chopped small fish (103.90 ± 1.10g) as prepared feed while live small fish resulted 3rd preference (81.89 ± 1.11 g). Si...
Zia-ud-Din1, Muhammad Nauman Aftab2, Tanveer Hussain3,*, Irfana Iqbal4 and Asma Zafar5
...>Indigenous Aseel female chicken (120 birds) comprising of four varieties (Lakha, Mushki, Peshawari and Mianwali) was fed for 42-days experimental period on antibiotic and probiotic supplemented ration. The results showed that the female growth, weight gain, FCR and economics varied significantly for feed supplements as well as for chicken varieties (P<0.05). The chickens given feed wit...
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...broiler growth curves of chickens with nonlinear regression analysis and grey prediction model. For this, the growth of 118 broilers was analyzed by using their weekly individual live weights from hatch to 49 day-old. In the analysis, nonlinear functions and Rolling-Grey Model (1,1) prediction method were used. The time-dependent growths of mixed sexes broilers were analyzed in the aspects of testing the parallelism of female and male growth samples, determini...
Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2*
...nal parasites in village chickens from four Townships (Mawlamyine, Nyaung U, Zay Yar Thi Ri and Katha) of Myanmar from April to June 2019. A total of 200 faecal samples were randomly collected from village chickens. Centrifugal sugar floatation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chicken
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...eviously both in broiler chicken and layer birds in Pakistan; however, their molecular characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular analysis of specific regions overlapping VP1-VP2 and VP2-VP3 genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those repo...
Abdul Samad1, Raheela Asmat2, Muhammad Naeem1, Hamida Ali3, Mohammad Zahid Mustafa1, Ferhat Abbas1, Jannat Raza1,3 and Muhammad Tauseef Asmat1*
...s consisting of beef and chicken meat, milk, salads and fresh vegetables were collected from retail markets of Quetta, Pakistan. Multiplex PCR was employed to identify the prevalent species and genes associated with virulence. Only 10 (1.25%) samples were found contaminated with Listeria monocytogenes. Milk samples were the most contaminated as 4 samples collected from retail shops or dairy farm were found positive for Listeria. While 2 samples o...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
..., dog, sheep, mouse, and chicken, respectively. It showed that MC1R gene had a high conserved type among different species. The goat MC1R protein has a molecular mass of 34.65 ku, an isoelectric point of 8.70, which is weakly alkaline, and contains seven transmembrane domains typical of cell membrane receptor proteins. Sequencing analysis of the black, brown and white different color MC1R genes of Nubian goats revealed that there are three SNPs in the gene seq...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
.../i>Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, including three novel mutations [SNP4 (A4812316G), SNP6 (C4813363A) and SNP7 (C4813618T)] and five known loci, were found within 3292 bp sequenced fragments. The allele frequency and genotype frequency of SNP...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...tudied the components of chicken feed previously, the purpose of this study was to observe the effects of chicken meat consumption on liver cells of rats. One hundred and twenty female Albino Wistar rats were randomly divided into four groups and separately for standard chow rat, commercial chicken feed, commercial chicken meat and organic
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...pathogen, a total of 360 chicken cloacal swabs and 15 water samples were gathered from different localities in Giza and Cairo Governorates. An additional 50 stool specimens were collected from individuals in contact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (PCR) as C. jejuni
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...ne competence of broiler chickens. In three experiments (n=240 chicks in each), the immune modulators vitamin C (500 mg/l), vitamin E (200 mg/l), dietary nucleotides (100 mg/l) and DNA (100 mg/l) extracted from chicken liver were separately offered on days 1-21, 1-42 and 22-42 in experiments 1, 2 and 3, respectively. Under standard broiler management conditions, birds were divided into five treatment groups of 48 with four r...
Imran Khan1, Muhammad Nawaz1,*, Aftab Ahmad Anjum1, Mansur-ud-Din Ahmad2, Adnan Mehmood1, Masood Rabbani1, Amina Mustafa1 and Muhammad Asad Ali1
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...ine efficacy, commercial chickens were vaccinated with La Sota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...VLDLR on reproduction in chickens, the haplotypes and diplotypes based on three genetic mutations (NC_006127.2:g.8467G>A, NC_006127.2:g.12321G>A and NC_006127.2:g.13876A>G) were constructed, and the associations of diplotypes with reproduction traits were assessed, their effects on gene expression were evaluated also. As a result, three haplotypes H1 (G-G-A), H2 (G-G-G) and H3 (A-A-G) were obtained, H1 was the main haplotype with a frequency of 91.75%...
Ishrat Perveen1, Yasar Saleem2 and Javed Iqbal Qazi3*
...nity. Ready-to-eat (RTE) chicken kabab samples of four commercially available brands (K, S, D and B) were analysed for the simultaneous determination of HCAs i.e. 2-amino-3-methyl-imidazo[4,5-f] quinoline (IQ), 2-amino-3,8-dimethylimidazo [4,5-f] quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhlP) using HPLC-UV detector. Quantitative analysis of the control samples (only thawed at room temperature) revealed pre...
Paul Oludare Adetunji* and Safiriyu Idowu Ola
Comparative Productive Performance among three Plumage Varieties of Noiler Hens
...us dual-purpose breed of chicken developed in Nigeria to meet national demands for food supply. The objective of this study is to compare the productive performance among three prominent plumage varieties of Noiler hens. For this purpose, a total of 90 pullets with three plumage colours (brown, black and barred) were divided into 3 replicates with 10 birds per replicate in each of the variety. Data were collected on the pubertal parameters (age at first egg an...
Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*
Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...production and increases chicken mortality. In human health, cases of this zoonotic agent are being reported and some cases showed fatal infection. The H9N2 is highly contagious, and transmission can occur from chicken to chicken, chicken to humans through the air, or sometimes from humans to humans. H9N2 belongs to alpha influenza virus genus of the fam...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...with VVND virus infected chickens. Clinical signs were observed in Japanese quails and mynas on 4th day post-inoculation. The major clinical signs were green yellowish diarrhea, ruffled feathers, anorexia and torticollis in all groups except Q3. The mean clinical scores and mean death time (days) were statistically non-significant (P>0.05) among all the groups. Higher mortality rate (75%) was recorded in mynas as compared to Japanese quails (50%)...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
... from commercial broiler chicken by three different methods i.e., congo red, test tube and air liquid interface coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm effect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantif...
Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3
...alo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their numbers. An urgent establish...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
Wahyu Widodo1,*, Imbang Dwi Rahayu2, Adi Sutanto3, Apriliana Devi Anggraini4, Harum Sahara4,
Sara Safitri4 and Abubakar Yaro5,6
...ve towards super kampong chicken (Indonesian native/local chicken - Gallus gallus domesticus Linnaeus, 1758) and to determine the most effective level of curcuma xanthorriza for the chicken. Completely Randomized Design (CRD) was used in this research through experimental design. Treatments in this research covered T0: Curcuma xanthorriza as feed additive 0.0 % (parameter), T...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
...d genes in H9N2 infected chicken’s erythrocytes have not been studied yet. So, the present study was designed to investigate the PG-related genes in erythrocytes of H9N2 infected chickens. In current study, qRT-PCR and Western blot analysis were performed to identify the expression levels of PG-related genes (COX-2, GSTA3, PTGDS, PTGES, PTGER3 and PTGER4) in erythrocytes of H9N2 infected chicke...
Abdul Muhaimin Wahab1, Basit Zeshan2,*, Naveed Ahmed2, Muhammad Afzal2 and Muhammad Naveed2
...he prevalence in broiler chicken farms in Kelantan state located at east coast region of peninsula Malaysia. Eighty cloacal swab samples were collected from 4 different broiler chicken farms in district Tumpat, Machang and Bachok. The samples were processed for identification of C. jejuni followed by PCR to detect the presence of C. jejuni. Overall, 65% in total (80 samples) of cloacal swab samples showed posit...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...e performance of broiler chickens. The study used 120 unsexed Hubbard one day old broiler chicks randomly divided into three groups of 40 chicks that were each further divided into four replicates of ten chicks. Extracts were introduced at 200 mg/Kg as feed supplement to two groups of broilers and the third group was kept as control (C) and offered a basal diet. The chicks were reared in floor pens bedded with saw dust litter. Measured feed was offered twice a...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...ducing E. coli in chicken, farmworkers, and water that is alarming and could lead to serious threats to both livestock and public health.
... 
NawalM. Abdulla1, M. Haroun1*,Mohamed A. Shalaby2and Ahmed A. Elsanousi2
... free (SPF) embryonating chicken eggs
(ECE),haemagglutination (HA), haemagglutination inhibition (HI) and mean death time
(MDT)used in the investigation to reveal virus identity and pathogenicity. Both NDV
isolates were found to be velogenic strains. Real time reverse transcriptase polymerase
chain reaction (rRT-PCR) used as a confirmatory technique, successfully amplified and
detected cDNA fragments ...
El-Habbaa, A.S.
...isolation on embryonated chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (17/28 of sheep isolates and 5/8 of goat isolates) using
...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...AI viruses isolated from chickens, Egypt during 2009-2011. Both inactivated and recombinant fowlpox virus expressing H5 (rFP-AI-H5) vaccines were measured. Vaccine efficacy was evaluated by percentage of protection from mortality, morbidity and reduction in virus shedding from respiratory and/orintestinal tracts. Despite the H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being...
Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5
...isolation in embryonated chicken eggs for three passages followed by ten successive passages in Madin Darby Bovine Kidney (MDBK) and identification of isolated virus was done by indirect immunofluorescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The results confirmed infection of calves with lumpy skin disease virus (LSDV) and showed that cal...
Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2
...tios of the two strains (chicken and duck strains)....
Hanaa A. Ahmed1 and Eman M. AboHatab2
...ane (CAM) of embryonated chicken eggs (ECE) SPF at 9-11 day and Madin Darby bovine kidney (MDBK) cell culture. Identification of virus by indirect immuno fluorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven...
Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***
...s bronchitis diseases in chickens....
Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*
...n free (SPF) embroynated chicken egg (ECE) showing the characteristic pock lesions for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV isolates were 2 and 2.75 when neutrized by FPV hyperimmune sera (HIS) while it was 2....
Safaa M. Mohammed*, Shahira A. Abdelwahab**,Fetaih,H⃰ ⃰ ,Neveen,R⃰ ,Abdel satar ,A⃰ and Mohamed S. Elshahidy**
...ed on CAM of embryonated chicken eggs and identified by agar gel precipitation test and polymerase chain reaction .Two main types of TKPV infection were observed in examined flocks ,cutaneous and diphtheritic forms. Morbidity rate ranged from 5-15%with no mortalities .75% of examined serum reacted positive to TKPV .All TKPV isolates were amplified using specific P4b primers and visualized by gel electrophoresis at 578bp....
Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1
...ect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 da...
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar
...of nine week old Hubbard chickens were vaccinated with two different commercial live attenuated Chicken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux strain vaccine through drinking water (DW). Then the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV vaccine forming three s...
Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2
...ruses were isolated from chickens and ducks in both live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 protein along all Egyptian isolates under study. Ala149, which is a pathogeneicity marker for interferon antagonism was found in the studied indicating capability of these viruses to transmit between different ...
Serageldeen Sultan1, Nabila Osman2
... virus (avian HEV) among chicken flocks in Egypt. A total of 510 serum samples were collected from broiler breeder (n=191), layer breeder (n=235) and commercial broiler chickens (n=84) from different farm flocks (24 flocks) in Egypt. These samples were examined for the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results revealed that the occurrence of avian HEV was 15.7% (80/510) and ...
Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5
...ane (CAM) of embryonated chicken eggs SPF at 9-11 day which showed pock lesions and CPE on MDBK cell culture. Isolated virus was identified by indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against Lumpy Skin Disease Virus. Further identifications were carried out by polymerase chain reaction (PCR). Results: 15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular...
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
... from 14-day-old broiler chickens with proventriculitis in Qaluobia province of Egypt in 2014. Objective: The isolate was identified by a real time RT-PCR targeting the nucleocapsid (N) gene with further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Re...
Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2
... occurring in vaccinated chickens with great economic losses. Objective: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of ND...
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
...rus for infection of SPF chicken. The tracheal swabs were collected
from diseased chicken for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of original HPAI virus was 1010.3 EID50/ml. The AI titers after experimental
infection of chicken and viral re-isolation were 1010.3 EID50/ml in ECE and 1.6×107 copies/ml...
Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir
...n free (SPF) embryonated chicken eggs ECE), chicken embryo kidney
cell (CEK) and in Vero cell line and was confirmed for IBV detection by Reverse Transcriptase Real
time PCR (RRT-PCR) using IB specific primers. Comparison of different isolation methods was done
by generating a standard curve by real time PCR.
Results: The results revealed that the propagated virus in CEK showed...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...
emberyonted chicken egg for three passages. The isolated samples were identified by EM and PCR.
Results: Five samples were positive and showed a typical pock lesion., the isolated strain was
identified as a sheep pox virus by EM and PCR. The virus was confirmed through sequence and
phylogenetic analysis of the RPO30 gene and open reading frame (ORF) 103 genes. The sequences
designated Sheep pox virus...
Abdou Nagy1, Fatma Abdallah1, Kareem Sweed2, Ahmed Salama3, Mohamed Omar4
... aMPV for non-vaccinated chicken and duck
flocks from six provinces of Egypt (Ash Sharqiyah, Ismailia, Qalyubia, Menufia, Sohaq and North-
Sinai) and determine its economic effect.
Methods: Serum samples were collected from non-aMPV vaccinated apparently healthy 40 chicken
(23 broilers and 17 layers) and 8 duck (6 Pekin and 2 Muscovy) flocks and then, tested with
co...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...lation revealed that all chicken groups vaccinated at 1 and 5 days of age demonstrated 100%
shedding at 3, 5, 7 and 10 days post challenge. However, groups 3 and 6 which were vaccinated at 10
days of age revealed difference in shedding pattern where group 3 (vaccinated with local prepared
vaccine) showed 100 shedding by rRT-PCR and 100%, 60% and 60 % of the chickens in tracheal swabs
...
Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid
... pathologic syndromes of chicken including
the both major syndromes, Viral Arthritis (VA) and Runting Stunting Syndrome (RSS), which have
great negative impacts on the poultry industry. Although ARV was reported in Egypt since 1984 but
only few studies figured out the implication of ARV only in the uneven growth of some Egyptian
chicken flocks.
Aim: Clinical and mol...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...
groups. All chicken groups were maintained at isolators along the experiment study. Blood samples were
collected for weekly for 4 weeks and antibody titers were determined by HI test. All vaccinated groups
were challenged 4 weeks post vaccination and tracheal and cloacal swabs were taken at 3, 5, 7, and 10 days
post challenge and tested by real time RT-PCR (rRT-PCR) and virus isolation and titration in SPF ECE.<...
Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2
...challenge pathway of SPF chickens
to estimate the protection percent of each vaccine against both classical and variant HPAI viruses.
While, the invitro method was done by the direct detection of the viral content and viral identity using
the rRT-PCR technique from final product of these inactivated vaccines.
Results: The data of this study showed that sufficient HA antigen content and similar HA sequences
...
Syed Maaz Nadeem1,*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh2, Arfan Ahmad2, Syed Nadeem Mansoor3 and Muhammad Anees4
...e cytological changes in chicken infectious anemia virus (CIAV) infected bone marrow in broiler chicken. Bone marrow samples were collected from proximal tibiotarsus and femur of each of the five healthy and CAV-infected flocks under natural condition. The bone marrow smears were fixed, stained with Romanowsky Giemsa Stain (RGS) and examined under microscope. Results showed a significant difference between the cellularity of...
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...mmune response of turkey chickens.
Methods: Four groups of bronzy turkey chickens were used in this study; group-1 received live
attenuated TRT vaccine, group-2 received inactivated AI vaccine, group-3 received TRT and AI
vaccines and group-4 served as non-vaccinated control. The humoral immune response evaluated
serologically by using hemagglutination inhibition test (HI), ser...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...the respiratory tract of chickens. In
Lebanon; there is a few data concerning the IBV among poultry flocks.
Objective: The aim of the present study is to detect the molecular characterize IBV in samples
collected from Lebanese flocks geographically distributed in 7 Governorates.
Methods: Blood sera, tracheal swabs and tissue organs were collected from 60 poultry flocks from
different ages, different t...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi
...talities were seen among chicken
flocks suggesting the influence of the AI H9N2 virus.
Objective: this study reports the circulation of H9N2 associated with mortalities in broiler sector and
decrease egg production in layer hens and breeders in Lebanon.
Methods: A total of 22 poultry flocks have been tested by real-time reverse transcription polymerase
chain reaction (rt-RT-PCR) for the presence of H9...
Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2
...train that isolated from chicken show where mutated strains from previously AIV circulating in Egypt from 2006 to 2008. Therefore, we recommend incorporation of these such isolates in any newly prepared AIV vaccines and vaccination program.
...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
...2 and Newcastle in layer chicken rearing is an
important issue.
Objective: To evaluate the protection duration for H9N2 and NDV post vaccination by inactivated
trivalent (H9N2, LaSota, M41 and Var2 IB) vaccine based mainly on repetition of challenge.
Methods: We prepared formalin inactivated combined vaccine for Avian Influenza (H9N2N),
Newcastle (La), and IB (M41 and Var 2) by using montanide 71 adju...
Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2
...and variant IBV in layer chicken rearing is an
important issue.
Objective: Evaluation of the protection duration for IBV (M41 and Var2) post vaccination by
inactivated combined (H9N2, LaSota, M41 and Var2 IB) vaccine based on laboratory tests and
repetition of challenge test.
Methods: We prepared formalin inactivated combined vaccine for Avian Influenza (H9N2N),
Newcastle (La), and IB (M41...
Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon
...stable ND vaccination in chickens. Sera obtained from forty (40) chickens per LGA during
pre-vaccination, post-vaccination and post-booster vaccination with NDV4 thermostable vaccine were
subjected to Hemagglutination Inhibition (HI) test.
Result: Antibody detection showed seroconversion in chickens 28day post vaccination and post
booster vac...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
... programs against NDV in chickens,
one using genotype VII inactivated and live NDV vaccine are available in the market and
second group used genotype II inactivated LaSota vaccine with live vaccine.
Method: NDV isolate designated NDV-GZ-986F-2015 was isolated from a commercial
broiler farm in Egypt and characterized as a virulent strain was used for challenge. Contact
chicks were added in both groups ...
Ahmed Ali Moryani1, Nasir Rajput1*, Muhammad Naeem1, Atta Hussain Shah1 and Hidayatullah Soomro2
...e performance of broiler chickens challenged with coccidiosis. A total of 450 broiler chickens were allotted into 6 groups (n = 75/group). Group A controlled without supplementation, Group B, C and D were supplemented with Aloe barbadensis (5ml/L), Ferulafoetida regal (500mg/L), Tamarindus indica (50mg/L), respectively. Group E and F were supplemented with a mixture of all 3 herbs/plants at 2ml/L; mixed with distilled water ...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1, Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...’s disease (MD) in chickens. Chicken erythrocytes are nucleated cells of myeloid origin which contribute to gases exchange, transportation and immunity. The objective of this study was to reveal the immune responses of MDV-infected erythrocytes of chicken by transcriptome analysis. Twenty specific pathogen-free (SPF) chickens were divided into two ...
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
...dy describes the role of chicken in harboring and environmental spread of ESBL and carbapenemase - producing Salmonella spp, which could pose a potential hazard to human and animal health. A total of 334 chicken meat samples, 197 eggshells and 160 human stool specimens were included in this study. The presence of salmonellae spp. was examined using bacteriological isolation and serological identification. PCR amplification o...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...asitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1,
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...ajor cause of illness in chickens with potential threats to human health. Chicken erythrocytes are known as most abundant circulating cells in the body which participate in inflammation and immune responses. Influenza viruses interact with the host cells at cell surface, leading to cell death in human and animals through apoptosis. Current study was designed to investigate the expression levels of apoptosis-related genes in ...
Theophilus Miebi Gbigbi* and Rodney Akpoviri Isiorhovoja
... profitability of exotic chicken enterprises in Delta State, Nigeria. A multistage sampling procedure was adopted to pick a sample of 120 farmers from whom data were elicited using structured questionnaire. Data were analyzed using descriptive and inferential statistics. The findings indicate that the average age of respondents was 43 years; majority (85%) of respondents were males with 16 years’ experience in exotic chicken
Jia Liu1, Zhen Wang1, Zifan Ning1, Ali Mujtaba Shah2,3,Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Yaofu Tian1, Diyan Li1,Gang Shu2,4, Lin Ye1 and Xiaoling Zhao1,*
..., and slow- growing (SG) chickens, however, the underlying molecular mechanisms responsible for the differences in post-hatch muscle development are unclear. Here, we report on the identification of several candidate genes that may modulate myofiber growth and thus explain some of the differences in the skeletal muscle phenotype of FG and SG chickens. We collected pectoralis major (PM) and gastrocnemius muscles (GM) on d 1, ...
Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
...rs. However, whether the chickens with different genetic background have the same response to feed restriction is not reported, herein we evaluated three levels of feed restriction effects on growth performance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weig...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
... Pradu Hangdum Chiangmai chickens (HPHC). PCR-RFLP protocols were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and ...
Ahmed M. Elbaz*, Engy F. Zaki, Morsy A. S.
... meat quality in broiler chickens. A total of four hundred and eighty 1-day-old Ross 308 chicks were randomly divided into four experimental groups: Control (CON), 5% of quinoa seeds (QS5), 10% of quinoa seeds (QS10), and 15% of quinoa seeds (QS15) groups. Results indicated that increasing inclusion levels of QS improved body weight gain, feed conversion ratio, and crude protein digestibility of broiler chickens. Including q...
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
...le that may be played by chicken-ready meals and food handlers in the transmission of different Salmonella serotypes to hospitalized patients in Assiut Governorate, Egypt, as well as, to assess their pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Sa...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...ineralization of broiler chickens. A total of 240-day old male chicks were randomly assigned into comprising four treatments with six replicates (n=10), which included control (basal diet), control +0.25% AZO, control + 0.50% AZO and control + kitasamycin (as AGPs). The results indicate that LBGW, ADG and FCR was significantly (P<0.05) improved in AZO 0.25% and 0.50% than the control. Eviscerated (EV), breast muscle (BM) and leg muscle (LM) were significant...
Keiven Mark B. Ampode1*, Federico C. Mendoza2
...ated immunity of broiler chickens. A total of sixty-day-old Cobb-broiler chickens were used in the study and arranged in a Completely Randomized Design experimental set-up with four dietary treatments. Each treatment was replicated three times, having five birds in every replication. The experimental rations containing graded levels of OP (0%, 1%, 3%, and 5%) were formulated and fed ad libitum in 42 days feeding trial. The r...
Amira Mahmoud Refaie1*, Marwa Hosni Abd El-Maged2, Hassan Abd El-Kriam Hassan Abd El-Halim2, Hanan Abd El-Rahman Hassan Alghonimy2, Sayed Ahmed Mohamed Shaban1
...al number of 240 Fayoumi chicken hens were classified into two equal experiments, 120 hens each (4 groups × 3 replicates × 10 birds). In each experiment, birds were divided into 4 treatment groups as follow: Normal control, positive control (0.0125% Butylated hydroxytoluene; BHT), 0.1% and 0.3% DPP, respectively. The DPP was used in two forms i.e., powder (DPP) for Exp.1 and aqueous extract (DPPE) for Exp.2. The results revealed that egg production...
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
...e passage in embryonated chicken eggs and further testing by haemagglutination test. Whereas, reverse transcription polymerase chain reaction (RT-PCR) using specific primers to amplify 1681 base pair (bp) of the whole NDV F-gene was carried out to confirm the presence of NDV and then sequenced. Our results demonstrated that, RT-PCR was successfully detecting 62 NDV samples. While, Sequencing and phylogenesis of six selected isolates revealed that, all isolates...
Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*
...n glucose homeostasis in chickens, we conducted the oral glucose tolerance test, and insulin sensitively test (OGTT and IST) in the fast- (FG) and slow- (SG) growing broilers. Each of 120 one-day-old males was raised in 10 batteries, respectively. In the OGTT test, ten broilers on d 42 fasted 12 h from each stock were randomly assigned into two groups: glucose and vehicle, respectively. The glucose group received a glucose syrup by oral gavage (2 g/kg BW), whi...
Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3
...parently healthy broiler chickens in Egypt. A total of 150 cloacal swabs collected from broiler chickens from farms and bird markets were subjected to bacteriological and biochemical examination. The results revealed that 92% (138/150) of samples were found positive for E. coli. The antimicrobial susceptibility testing of 72 confirmed E. coli isolates against seven quinolones and fluoroquinolones antibiotics revealed that 62...
Pham Tan Nha1*, Nguyen Thi Kim Dong2, Le Thu Thuy1
... performance of Tau Vang chicken in 7-14week old period. It was a completely randomized design with 5 treatments corresponding to 5 diets and 4 replications with 10 birds per experimental unit. The treatments were the different ginger supplement levels of 0.1. 0.2. 0.3 and 0.4 % (in DM) to basal diet. Corresponding to the G0, G0.1, G0.2, G0.3 and G0.4 treatments. The results showed that the daily intakes of DM, OM, CP and EE were signiificantly higher (P<0....
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2
... disease particularly in chickens. Thus, this preliminary study was performed to identify intestinal fungal population in immunosuppressed chickens. Cloacal swabs were collected from freshly dead chickens suffering from infectious bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus...
Ho Le Quynh Chau, Than Thi Thanh Tra, Duong Thi Huong, Du Thanh Hang, Le Thi Thu Hang, Vo Thi Minh Tam, Dinh Van Dung*, Ho Trung Thong
...pression of two coloured chicken groups. A total of 480 one-day-old chicks (240 Ri hybrid and 240 Luong Phuong) of uniform body weight was divided into a 2 × 3 factorial arrangement of treatments with four replicate cages of 20 chicks per treatment. Two factors include 2 chicken groups (Ri hybrid-slow-growing strain and Luong Phuong-fast-growing strain) and three dietary SID methionine concentration (low, adequate, hig...
Amir Z. Allam, Hayam A. Mansour, Nermeen M.L. Malak, Heba H.S. Abdel-Naeem*
...the bacterial quality of chicken meat patties during frozen storage at –18 °C for 12 weeks by enumeration of aerobic plate count (APC), psychrotrophic, Enterobacteriaceae, and lactic acid bacterial (LAB) counts. The results revealed that the sole effect of T, CL, and O oleoresins caused non-significant reductions in all investigated bacterial counts. Among the treated groups (T, CL, or O alone), it was found that O oleoresin achieved the highest redu...
Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1*
...ibuting contamination in chicken samples that were collected from broiler farms and slaughterhouses using conventional culturing, biochemical, and serological identifications versus molecular detection. A prospective study was designed to last for six months from March 2021 to the end of August 2021. A total number of 126 chicken samples (100 samples from five broiler chicken farms and 26 ...
Syed Haseeb Ahmed Shah, Irfan Shahzad Sheikh, Abdul Samad, Muhammad Kamran Taj, Mohammad Masood Tariq*, Majed Rafeeq, Sumaira Fazal, Niamatullah Kakar, Sabeera Afzal and Asadullah
...s of bacteria in broiler chicken. These anti-microbial agents are capable of destroying the metabolism of microbes and can alter certain properties of bacterial cellular and metabolic activity which results in impaired growth or death of these bacteria. This study was planned to find out the effect of AGPs in broiler chicken. For the purpose one day old (n=432) broiler chicks were used in the experiment. Nine groups comprisi...
Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*
Mian Abdul Hafeez1*, Adeel Sattar2, Faiza Aslam3, Muhammad Imran3, Kamran Ashraf1, Rashid Zia2 and M. Muntazir Mehdi1
...uprofen with clopidol in chicken coccidiosis....
A.H. Alqhtani1, A.S. Alharthi1, N.J. Siddiqi2, S. Zargar2 and A.M. Abudabos1*
...his study four groups of chicken challenged with Clostridium perfringens were fed on Neoxyval, GalliPro, TechnoMos and GalliPro + TechnoMos. A group fed on normal diet was used as control and the other challenged with bacterium was maintained. Three hepatic enzymes viz., lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increased after feeding on growth promoters. However, total serum protein, gamma glutamyl trans...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
... antibiotics for raising chicken in Egypt. The occurrence of antibiotic residues in broiler chicken is the chief causes of the development of antibiotic resistance in food-borne pathogens. Antibiotic resistance is setting the achievements of present day medication at chance. Therefore, in this study 300 chilled broilers carcasses were collected from different poultry markets in Ismailia City, Egypt to evaluate qualitative, q...
Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad
...enic bacteria in broiler chickens. A total of 160 day-old Ross chicks were assigned to four groups, containing 0, 0.5,1 and 1.5% GA along with basal feed for 42 days. A completely randomized design (CRD) was followed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, feed conversion ratio (FCR), Eu...
Ali Raza1, Muhammad Sharif1*, Fawwad Ahmad1, Asghar Ali Kamboh2, Muhammad Saeed3, Muhammad Ashraf1 and Asad Ullah Hayder1
...er parameters in broiler chicken. Diet A was without vitamin D and phytase supplementation (control). Diets B, C, D, E, F and G were supplemented with phytase at the rate of 400 FTU/kg, 800 FTU/kg, vitamin D 4000 IU/kg, 8000 IU/kg, phytase 400 FTU/kg + vitamin D 4000 IU/kg and phytase 800 FTU/kg + vitamin D 8000 IU/kg of feed, respectively. Each diet was fed to 30 broiler birds from post hatching to day 35. Feed intake was higher (P < 0.05) in birds offered...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...meat products as grilled chickens, grilled beef kofta, and cooked beef meat sold in Assiut city restaurants, and among food handlers working in the same food premises. In addition to the sequencing of 23S rRNA gene and phylogenetic relation between S. aureus isolated from meat products and food handlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of <...
Zulu Blantina Fangele, Tyasi Thobela Louis, Gunya Busisiwe*
...sp;...
Rafiqul Islam1, Nasrin Sultana1*, Md. Abu Hadi Noor Ali Khan2
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...aturally infected native chickens. Thus, bursa specimens were collected from two IBD vaccinated native broiler chicken flocks showing clinical signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly characterized using reverse transcription polymerase chain reaction (RT-PCR) technique and gene sequencing ...
Shewangizaw Wolde1,2*, Tadele Mirkena1,3, Aberra Melesse1, Tadelle Dessie4, Solomon Abegaz5
...owth performance of male chickens and carcass traits of old hens, and genotype and age effects on carcass traits of male chickens. A total of 150 Normal Feathered local chickens (LL), 150 Sasso-RIR (SRSR) and 150 their F1 crossbred (LSR) chickens were hatched and reared on station until 18-weeks of age. Then, 32 males of each genotype were evaluated for ...
Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3*
...s characteristics of Noi chickens. A total of 183 birds were examined in the study. Body weight was recorded individually at days 1, 35, 63, and 91, and carcass parameters were determined at 91 days for all chickens. For determining single nucleotide polymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A polymorphic site was found in the population, with the frequencies of 11, 12, and 22 geno...
Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2
...nt> (KBC) is a local chicken in West Sumatra as a crowing chicken. This study aims to obtain primary data on population structure and management of KBC maintenance in in-situ areas. The respondents of this study were fifty-seven farmers who kept the KBC. The method was survey method and purposive sampling to determine the respondents. The observed variables were breeder profile, maintenance management, number of KBC, act...
Taiwo Oladoye Akande*, Dayo Johnson Ogunyemi, Priscilla Funmilola Okunlola, Emmanuel Owolabi, Odetayo Olakanmi
...the organ weights of the chickens. The fat deposition was substantially reduced (P<0.05) in birds with PFAs. The blood triglycerides, cholesterol, and low-density lipoprotein (LDL) were lower (P<0.05) in groups fed with PFAs. All the PFAs significantly increased the HDL above the value obtained in control. No mortality was recorded throughout the experimentation period. It was concluded that the three PFAs and their mixture used in this study improved th...
Sutopo Sutopo, Dela Ayu Lestari, Edy Kurnianto, Asep Setiaji*
...ont>s. The number of chicken used in each variety of the male Red comb Kedu and Black Comb Kedu Chickens were 5 and 4 heads, respectively. Meanwhile, the number of female was 24 and 20 heads, respectively. A total of 901 eggs were collected during the period of 11 weeks. Egg weight (EWG) and body weight (BW) were weighed individually. BW recorded were BW0, BW15, BW30, BW45, and BW60. The average daily gain was counted as...
Jumshaid Iqbal1, Muhammad Sharif1*, Muhammad Nadeem Suleman2, Muhammad Saeed3, Fawwad Ahamd1, Asghar Ali kamboh4, Tugay Ayaşan5 and Muhammad Arslan3
...stomorphology in broiler chickens. One hundred and twenty day-old broiler chicks were randomly distributed into four treatment groups with three replicates per treatment (10 chicks per replicate) under Completely Randomized Design. Four iso-nitrogenous and iso-caloric diets i.e. A, B, C and D were supplemented with a natural growth promoter (NGP) at the rate of 0 (control), 1.5, 3 and 4.5g/kg of feed, respectively. Feed consumption (FC) and weight gain (WG) we...
Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...us of domesticated (i.e. chickens, peacock, pet birds) and wild birds, which is excreted in the fecal material of infected birds. The house fly, Musca domestica L. can gain entry into the poultry flocks and will feed on poultry droppings and potentially serves as a mechanical vector for Newcastle disease. During the current study, houseflies were captured and examined for the presence of NDV. A total of 80 samples were collected from eight different poultry fa...
Thobela Louis Tyasi1*, Lebo Trudy Rashijane1, Kwena Mokoena1, Kagisho Madikadike Molabe1, Madumetja Cyril Mathapo1, Victoria Rankotsan Hlokoe1, Lebelo Joyceline Selala1, Masixole Maswana1 and Lubabalo Bila2
...s a reproduction tool in chickens and a valuable food source for humans. The objective of this study was to examine the effect of egg weight (EW) on egg quality traits such as egg length (EL), egg diameter (ED), yolk weight (YW), albumen weight (AW), shell weight (SW), shell index (SI), yolk ratio (YR), albumen ratio (AR) and shell ratio (SR). Potchefstroom Koekoek layer genotype eggs (n = 200) were used. Pearson correlation and analysis of variance (ANOVA) we...
Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1
...ler, sonali and backyard chicken. AI outbreak is observed every year since its first report in 2007 in Bangladesh. This virus has mutated several times and thereby changed its genetic clades. The prevalence of ND is highest in October and in 0-3 months aged chickens. AMPV generally infects the upper respiratory tract of poultry, which has been isolated for the first time in Bangladesh in 2016. Alternatively, adult
Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6*
...the interactions between chicken bone marrow-derived dendritic cells (chBM-DCs) and FWPV and its recombinant viruses are limited compared to other avian viral infectious diseases. Therefore, this study characterised the response of chBM-DCs to infection with the wild-type (WT) FP9 strain of FWPV compared to a recombinant FWPV carrying the H5 gene of avian influenza virus (rFWPV-H5) using morphological and immunophenotypical observations. The results showed tha...
Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*
... each group contained 40 chickens that were fed outdoors in an activity area of 63 m2 with free food and portable water. The CK was fed basic diet, and the experimental group added 1%, 3% and 5% linseed oil to the basic diet (TP1, TP2, TP3), respectively. On the 10th, 25th and 40th day of the formal trial, 36 eggs were randomly selected from each group, of which 12 were used to detect egg quality and 24 to detect monounsaturated fatty acids in eggs. The result...
Lopamudra Samantaray*, Yashaswi Nayak
...e performance of Broiler chickens. The study used 280; 70-day-old chicks. The birds were divided into seven experimental groups; one control and six were fed on phytobiotics EOs at rates of 1% and 0.5% and monitored for 8 weeks. The results revealed that the inclusion of 0.5% black pepper and 0.5% fennel combination, as well as a 1% turmeric, significantly (P < 0.05) improved body weights in birds. Black pepper and a combination of turmeric and fennel revea...
Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1*
...ollected from 68 broiler chicken flocks with variable mortality rates in four Egyptian governorates (Fayoum, Beni-Suef, Giza, Minia). All samples were cultivated on PPLO media and detected under stereo-microscope for fried egg colonies. Forty-three samples were detected as Mycoplasma spp., using PCR for species identification, MG, MS, and Untyped strains, with a high prevalence of MG (27.9%) and lowest for MS (8.8%). An rRT-PCR used for detection of AIV-H9N2 ...
Weam Mohamed Baher*, Gamilat A. El said
...e the hygienic status of chicken meat including breast and thigh collected from rural and urban localities in Egypt. Evaluation of the hygienic status of chicken meat was done via estimation of total bacterial count (TBC), most probable number (MPN) of coliforms, total staphylococcus count (TSC), and total mold count (TMC). An experimental trial for the improvement of the hygienic status of chicken
Mohamed El-Henfnawy*, Essam A. El-Gendy, Ahamed M. El-Kaiaty, Mostafa Helal
...>...
Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*
...eed additives on broiler chicken performance, carcass characteristics, blood biochemical parameters, immunological condition, and gastrointestinal health. Total of 240 male Arbor Acres broiler chicks were randomly divided into four groups, each with six replicates of 10 chicks. Group I fed diet consisted of a basic diet with no additives (control). Groups II, III, and IV fed diets consisted of a control diet supplemented with 1.5g sodium formate/Kg (SF), 1.5g ...
Tri Untari1*, Sitarina Widyarini2, Michael Haryadi Wibowo1, Marla Anggita1
...ect of red ginger to the chicken livers and kidneys. Antibody titers in group I, II, III and IV were determined as HI (log)2 average and were recorded 3.81, 2.91, 3.13 and 0.95, while the geometric mean titers (GMT) were 13.9, 8.6, 7.5 and 1.9 respectively. The unvaccinated groups (V, VI, VII) exhibited 0 and 1 as HI (log)2 and GMT respectively. There was a significant difference between the measured antibody titer of group I and other groups (p<0.05). No...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...athogen free embryonated chicken eggs (SPF-ECEs). The dead embryos showed hemorrhages, congestion and dwarfism. The collected allantoic fluids were examined later by haemagglutination (HA) and haemagglutination inhibition (HAI) test. The allantoic fluids of 5 pooled samples expressed positive using specific Newcastle disease virus (NDV) antiserum. The NDV field isolates were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and partial sequ...
Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby*
...dly, thus, 200 different chicken samples were examined for isolation of E. coli and for colistin resistance gene. Eighty-three selected samples (chicken breast) were subjected to detection of colistin residues by HPLC (High-performance liquid chromatography). In this research, we used polymerase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutati...
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
... serotype O6 infected in chickens. A total of 420 broilers were randomly assigned to seven treatment groups. The negative control (NC) birds received no E. coli or phages, whereas the positive control (PC) birds were infected with E. coli only. The NC+MHH6 and NC+PR2 treatments received 109 pfu/ml of phages MHH6 or PR2, respectively; whereas the PC+MHH6, PC+PR2, and PC+MHH6PR2 groups were infected with 107.1 cfu/ml of E. coli strain O6 and treated with 109 pfu...
Ghulam Abbas1*, Muhammad Arshad1, Muhammad Saeed2*; Safdar Imran3, Ashgar Ali Kamboh4, Duraid KA Al-Taey5, Muhammad Asad Aslam1, Muhammad Saeed Imran6, Muhammad Ashraf3, Muhammad Asif7, Abdul Jabbar Tanveer8, Razia Abdul Majid Qureshi9, Maria Arshad1, Hussain Ahmed Khan Niazi1, Muhammad Tariq10, Sikandar Abbas1
...% increase in the FCR of chicken. Lactic acid fed 0.3 g/kg diet reduced Escherichia coli and Salmonella significantly in the cecum of broilers. Butyric acid fed 500 g/t feed significantly increased eggshell thickness, eggshell weight, and calcium concentrations in bones and reduced ammonia concentrations in the caecum. Egg production was increased upto 9.84% by the supplementation of OA. Organic acids also have promising effect on gut health evidenced by posit...
Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2**
...identified among broiler chickens in Luxor governorate in 2016. Five hundred (n= 500) samples were collected from fifty farms (5 tracheal and 5 cloacal pools from each farm) have been examined by real time RT-PCR for the prevalence of AIV H9. The results revealed that ten farms (20%) were positive for AIV (H9N2). Three isolates with high CT values have been selected for complete sequencing of hemagglutinin gene. Amino acid sequences showed that these isolates...
Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2
...r the rearing of broiler chickens for better production....
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
...at production of broiler chickens are indicators to assess carcass quality. This study was conducted to evaluate the carcass characteristics and production of pure meat broiler chicken circulating in the traditional markets of Lombok and Sumbawa based on the Indonesian National Standard. The material used was 191 heads of broiler chickens with a slaughter age of 45-49 days. Ninety heads fr...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
Ahmed Abd El-Salam1*, Yasser Hamouda1, Salah El-Safty2, Ahmed El-Attar2
...lity is critical. In the chicken industry, incubation is one of the most critical variables impacting profitability. As a result, the study’s goal is to see how egg-shell quality differs between strains. From the two egg type breeder strains, 240 hatched eggs (120 eggs per strain) were picked at random. The layer breeder flocks were tested at four different ages (22, 33, 43, and 64). The purpose of this study is to investigate the differences in egg shel...
Fei Wang1, Xiaofen Hu1, Feng Wen2, Xiaoen Tang3, Shanshan Yang1, Shengwei Zhong1, Zuohong Zhou1, Xu Yuan1 and Yong Li1,*
...ive characteristics with chicken, turkey, and Taeniopygia guttata. Besides its conservative evolutional characteristic, the simple structure, subcellular localization, hydrophobicity and hydrophilicity indirectly account for its extensive distribution and various functions as expected....
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
... growth plate in broiler chickens. So, it is effectual through rectifying the oxidative imbalance and liver damage....
Ahmed A. Mohammed1, Madeha H. A. Darwish1, Eman A.A. Negm2, Ayman S. Abdel- Maguid3
Effects of Antibiotics on Growth Performance, Immune Response, and Intestinal Microflora of Broilers
Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5
...rty-five day-old broiler chickens were randomly divided into five treatments and housed under identical husbandry conditions. Antibiotic-free poultry feed was procured from a commercial feed mill. The antibiotics were mixed in the feed as per the manufacturer’s instructions and offered ad libitum to the chickens for the entire study period. The chickens were vaccinated for NDV and AI...
Muhammad Hanif Chandio1,2*, Naeem Tariq Narejo2, Muhammad Farooq Hassan3, Faheem Sadar4, Nasiruddin Shaikh1, Bushra Ainy Dars5, Ghulam Abass6, Asma Fatima6 and Shahnaz Rashid6
...sh were fed all parts of chicken (APC) with 10% of their body weight. The results of the growth trial indicated moderate variation among the treatments that, 10 fish/m2in T-I exhibited maximum growth (64.6 g) with 100% survival ratio as compared to other treatment (II and III), 15 fish/m2 (35.0 g with 90% survival) and 20 fish/m2 (16.4 g with 80% survival), respectively. The water quality parameters were monitored on fortnightly basis, such as temperature valu...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...ewcastle disease (ND) in chickens. Owing to its highly conserved nature among NDV strains and also due to its pivotal role in the viral life cycle, the M protein can be employed as a promising diagnostic antigen for reliable detection of NDV infection in chickens. In the present study, we have devised a strategy for extraction and solubilization of the NDV M protein from Escherichia coli in a single step using a non-ionic de...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...ltimately protecting the chicken from pH-sensitive pathogens. The purpose of this study was to define the bactericidal action of organic acids on Campylobacter jejuni, individually and in combination. Total 120 broiler chickens were randomly distributed in ten groups. The groups included negative and positive control, pure organic acid group and commercial organic acid formulation group. Excluding negative control group, all...
Tarek A. Wrshana1, Yousry A. Dowidar1, Bahgat A. El-Fiky2, Ali M. El-Rify1, Walaa A. El-Sayed3, Basem M. Ahmed4*
... native Egyptian Fayoumi chicken. The MSC with the surface marker CD105 (CD105+) were magnetically separated and infected with virulent Newcastle disease virus (vNDV). The virus-induced multiple changes at the cellular and ultrastructural level in the infected cells, and it was able to maximize the production of interferon-gamma (IFNγ) and interleukin 2 (IL2), interleukin 6 (IL6) and interleukin 12 (IL12). In conclusion, our data represent a preliminary ...
Djoko Kisworo*, Martina Safitri, BRD Wulandani, Bulkaini, Syamsuhaidi
...act on the shelf life of chicken eggs. A Completely randomized design (CRD) was used in a 3 x 3 factorial pattern with five replications. The Factors were the level of SSF (0%, 10%, and 20%) and storage time (2, 4, and 6 weeks). Data were analyzed according to the analysis of variance followed by the Least Significant Difference test (LSD). The parameters measured were the egg’s weight, air cavity, pH, and Haugh Unit of chicken
Abdulaziz A. Alaqil1*, Mohammed I. Buhaya2
Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy*
...aphylococcus aureus from chicken and duck farms production at a titer of 3×106 CFU (Colony Forming Unit) /cm2 in the absence and presence of organic matter (O.M). The results showed that the efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000® was the most powerful disinfectant against field pathogens, followed by G7®, Pron-Tech®, and Alkadox®, while Biodine® was the w...
Irfan Shahzad Sheikh1, Syed Haseeb Shah1, Niamatullah Kakar2*, Mohammad Masood Tariq1, Muhammad Essa Kakar3 and Muhammad Zahid Mustafa1
...al parameters in broiler chicken. AGPs supplement alone and in combinations were provided as pre-starter (days 1-12), starter (days 13 to 24) and finisher (day 25 to 42) feed. Standard broiler rearing temperature was provided in the shed. Results showed that the OXY and TP had significant (P<0.05) effect on physiology of blood parameters, mainly on hemoglobin (Hb) and erythrocytes (RBCs) in all treatment groups treated till day 42 compared with the control ...
Muhammad Halim Natsir*, Osfar Sjofjan, Yuli Frita Nuningtyas, Danung Nur Adli, Lizar Basnelda, Dimas Frisky Apriyanto, Wahyu Tri Utami
...ial population of Lohman chickens. A total 256 Lohmann chicken were used on the experimental diets. The Fountain clerodendrum and avocado seed flour were enhanced with nanotechnology 500 mm. The two-way nested Anova with eight treatments and four replication was employed, with each experimental unit consisting of eight Lohmann broiler chickens. The treatments consisted of B1T0 = basal diet...
Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1*
...as evaluated versus five chicken Eimeria species oocysts. The immature oocysts were exposed to 5, 10, 20, and 40 ppm, each for 1, 3, 6, 9, 12, 24, and 36 h. Percentage of sporulation inhibition in oocysts and comet assay were used to investigate the C-Oo.Nc destructive effects. The infectivity of sporulated oocysts exposed to LC50 & LC90 were evaluated by In vivo inoculation of broiler chicks. The results demonstrated that the increase in the rate of sporu...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2
...o 260 commercial broiler chickens in 13 groups; 20 birds each. At 28 days-old, all groups were challenged with NDV genotype VII 1.1 strain “NDV-CHICKEN-EGY-ALEX-NRC-2020” to evaluate the protective immunity of applied protocols. Humoral immune response, clinical signs, post-mortem gross lesions, growth performance as well as mortalities are all recorded and evaluated. All vaccination protocols were able to induce...
H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1
... was recorded in breeder chicken farms (10.2% and 29.3% respectively). The isolated Salmonella species showed high resistance (100%) against doxycycline, ampicillin, and amoxicillin, while E. coli species showed resistance (90%) against oxytetracycline, doxycycline, ampicillin, and amoxicillin. Both Salmonella and E. coli species were highly susceptible to gentamicin. Gene tetA and blaSHV were detected in Gene tetA and blaSHV...
Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3
...xed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was i...
Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti
...e tract of BSF larvae in chicken manure and palm kernel meal substrates was isolated and taxonomically identified using the 16S rRNA sequence homology and molecular identification. The LAB isolates were also tested for antimicrobial and hemolysis activities. Five isolates from BSF larvae reared on chicken manure substrate and three isolates from BSF larvae reared on palm kernel substrate showed a gram-positive bacteria chara...
Midrar Ullah1, Nail Chand2, Abdul Kabir3, Muhammad Rasheed4, Hubdar Ali Kaleri5, Saqib Kakar5, Mubarak Ali4, Deepesh Kumar6, Asad Ali Shah1, Amir Shahb1, Muhammad Amir Khan1, Raza Ali Mangi7, Abdul Wahid Solangi5, Rameez Kaleri5,8*
Zahin Anjum1*, Mubashra Tarana1, Shaista Ali1, Faryal Yousaf1, Amina Rahat1 and Sumbla Yousaf2
...e tissue) of the broiler chicken for absorption of four heavy metal contents (Cu, Zn, Pb and Ni). In addition, analysis of five electrolytes (Ca, Na, K, P and Mg), moisture content, protein, lipids and ash were carried out. The samples were obtained from five different sites of the University Campus, Peshawar- Pakistan. The Atomic Absorption Spectrometry was employed for determination of Cu, Zn, Pb, Ni, Ca and Mg. Furthermore, a spectrophotometer was also used...
Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari
Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja
...t morphology of Ross 308 chickens from day-old to 6 weeks of age. 320-day-old Ross 308 broiler chicks were assigned to a 2 (sex) × 4 (dietary treatment levels) factorial arrangement in a CRD, having 8 treatments, replicated x4 with 10 chicks/replicate. AKLM inclusion levels were at 0, 0.5, 1.0 or 1.5g/kg dry matter (DM). Weekly feed intake (FI), live weight (LW), and growth rate (GR) were measured to obtain the feed conversion ratio (FCR). Gut character...
Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2
...ion into the embryonated chicken eggs. The direct detection through RT-PCR confirmed H9 gene from cloacal swab samples in 6.9% of the seropositive sample while we could not confirm any of the oro-pharyngeal samples for H9 gene through direct molecular detection. The cultivated oro-pharyngeal and cloacal swab samples were not found positive upon re-confirmation from allantoic fluid through RT-PCR by using same specific set of primers. This study concludes that ...
Ahmed H. Abed1, Ahmed Orabi2*, Wafaa Hassan3, , Enas Hammad4 , Ghada Gaber5
...iating effect on broiler chickens’ immunity challenged with E. coli. One hundred and twenty broiler chicks were separated into eight equal groups in an in vitro experiment (15 birds for each group). A negative control group was the first group. There were 3 groupings were given immune modulatory agents (lectins, probiotic, and essential oil) for five days without being challenged; Immune modulatory agents were given to the remaining three groups, who wer...
Tlou Grace Manyelo1,2, Nthabiseng Amenda Sebola1, Jones Wilfred Ng’ambi2, Monnye Mabelebele1*
... of indigenous Boschveld chickens. A total of 200 one-day-old indigenous Boschveld chicks were randomly allocated to five treatments in a completely randomized design each with four replicates of ten chicks. Amaranth leaf meal (ALM) levels evaluated in this study were 0, 5, 10, 15, and 20% with recording weekly body weights, feed intakes, and feed conversion ratios. Gut organ weights, lengths, pH, and meat characteristics were determined, and the general linea...
Hemat A. Abdel Magied*, Heba H. Habib, Amany H. Waly, Ahmed Fadl, Sayed M. Shalash
...y of Arbor Acres broiler chickens. Two experiments were conducted in current study; experiments 1 (EXP1) evaluated the effects of PE and/or P supplementation to standard corn-soybean broiler diet (STD). while experiment 2 (EXP2) examined the PEP supplemented to different levels of low CP (low 10 or 15 %) and low ME diets (lower 100 or 150 kcal/kg) than control (L10%-100, L10%-150, L15%-100, L15%-150). The obtained results were: the EXP1 showed that significant...
Andy1,4*, Ratmawati Malaka2, Sri Purwanti3, Hikmah M Ali2, Tutik Lusyta Aulyani4, Danung Nur Adli5
...obial profile of broiler chickens. A total 100 broiler chickens were reared for five weeks on the experimental diets. A general linear models with five treatments and four replications was employed. The treatments consisted of T0 = control, T1 = basal diet + acidal, T2 = basal diet + 0.5% encapsulation of liquid smoke, T3 = basal diet + 1.0% encapsulation of liquid smoke, T4 = basal diet + 1.5% encapsulation of liquid smoke....
Sugiharto Sugiharto*, Ikania Agusetyaningsih, Endang Widiastuti, Hanny Indrat Wahyuni, Turrini Yudiarti, Tri Agus Sartono
...n CONT, HSD-P and HSD-PC chickens consumed more (P < 0.05) feed than HSD chickens. Body weight and weight gain were lower (P < 0.05) in HSD compared to the CONT, HSD-P and HSD-PC groups. The European Production Higher Efficiency Factor (EPEF) tended (P = 0.06) to be higher in HSD-PC than in HSD chicks. While there was no significant effect of the treatments on complete blood counts and serum biochemical parameters, ser...
Istna Mangisah1*, Heni Rizqiati2, Nyoman Suthama1
...starter. Day-old broiler chickens with an average body weight of 45.61 ± 3.46 g, a total of 160 birds, were placed evenly in 20 experimental plots. The treatment tested was the LDTE level in 100 grams of feed, LDTE0 = without LDTE, LDTE1 = 1 mL, LDTE2 = 2 mL, and LDTE3 = 3 mL. The treatment was given for 21 days, starting from d-1 until d-21. Intestinal microbial population, weights of digestive and immune organs, nutrient digestibility, and growth per...
Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*
... (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented with NC+ (500 and 750 powder phytase units (FTU)/kg) and G, H supplemented with NC+ (500 and 750 liquid phytase units (FTU)/kg) respectively. Pelleting...
Ahmed Rizk1, Gomaa Said Ramadan2*, Alaa Eldin Abdou3, Ossama Ahmed El-Weshahy1, Omer Sayed Rashed1
...r Tanta G-1 and Mamourah chicken strains was 866.45 grams and 558.75 grams in the first generation. In the second generation, the Tanta G-1 line’s body weight was 1006.50 grams against 555.10 grams for the Mamourah strain, while in the third generation the average body weight in the Tanta G-1 line was 1068.93 grams versus 551.25 grams in the Mamourah strain at the age of 8 weeks. This difference between the two lines was significant (P≤0.05). Body w...
Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan
...FB1) toxicity in broiler chicken during 1 – 42 days of age. Eight hundred broiler chicks were arbitrarily assigned for 8 treatments of 5 replicates (each contained 20 chicks). On the other hand, chickens were offered the basal dietas Negative Control (NC, group 1); the other three groups 2, 3, and 4 were fed NC supplemented with 0.5% HSCAS, SC and HSCAS plus SC respectively, group 5 chicks were fed a basal diet contami...
Pham Tan Nha*, Le Thu Thuy
...the performance of local chickens in the south of Vietnam. It was (3*2*3 factorial) with 2 factors. Factor fermentation: Yeast-fermented maize (YM), Yeast-fermented broken rice (YR), Probiotic fermented maize (PM). Factor protein level: 20% (CP20) and 16% (CP16). One hundred and eighty Noi chickens at 6 weeks of age (334±9.5 g/bird), and 10 birds per unit (balanced sex) were in the treatment. All treatments were added...
Samantha Shiuan Erl Lee1, Nurul Huda2*, Yetti Marlida3, Eng-Keng Seow4
...functional properties of chicken and duck egg whites were studied. Egg whites were treated with ultrasound generated by sonicator (frequency 40kHz) for 15 min, and their colour, pH, expressible moisture, folding test, gel strength, texture profile analysis, foaming capacity and stability, and emulsification stability were compared. Ultrasound treatment had caused a significant decrease (p<0.05) in pH and expressible moisture, apart from improving the gel st...
Andi Tenri Bau Astuti Mahmud1, Lellah Rahim2, Muhammad Ihsan Andi Dagong2*, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2
...ual feed intake (RFI) in chickens, which is usually calculated during the growth period, is a measure of feed efficiency that does not depend on the level of production. The study aimed to evaluate the growth performance, carcass quality, physical meat characteristics, and sarcomere properties of Kalosi chicken, which were grouped according to high and low Residual Feed Intake (RFI) phenotype. This study used 64Kalosi
M. Mansoor Javed*, M. Aslam Mirza, M. Aziz ur Rehman and M. Saif ur Rahman
...on and growth in broiler chickens. Full-fat soybean was prepared by subjecting dehulled soybean to different temperatures i.e. 90°C, 100°C and 110°C for various resident tines i.e., 15 min, 30 min and 45 min in a multitier commercial toaster. Each product was analyzed for proximate composition, pepsin digestibility, KOH solubility and urease activity prior to inclusion in the experimental diets. A day old Cobb-500 broiler birds (n=432) were purchas...
Yar Muhammad Jalbani1,2*, Muhammad Farooque Hassan2, Kiran Nazish2 and Yang Shuming1
... storage life of chilled chicken meat to avoid economical losses and provide the basis for standard regulations assessing meat storage life. For analyzing the storage life of chilled chicken meat rapidly, the samples were analyzed on Day 0, 3, 5, 7 and Day 10 using near infra-red spectroscopy approach combined with chemometrics tool of data driven soft independent modeling of class analogy. The meat samples from day 0 were s...
Amal G. Abdelrahman1, Nabil A. Yassien1, Hussein M.H. Mohamed1, Khaled S. Tolba2, Heba H.S. Abdel-Naeem1*
...y nutritious value-added chicken meat patties. In this context, five groups of spent hen meat patties were formulated as follows: The first and 2nd groups were treated with 5 % and 7 % of kiwi extract, the 3rd and 4th groups were treated with 5 % and 7 % of pineapple extract, meanwhile, the 5th group was treated with a mixture of those extracts (5% kiwi + 5 % pineapple) in addition to the control group. All groups were kept in frozen storage at ‒18 °C fo...
S. Feroza1, A. G. Arijo1†, F. M. Bilqees2 and M. S. Phulan1
...ical changes in liver of chicken, experimentally induced with Ascaridia galli round worm. The postmortem examinations of infected chicken revealed histo-pathological lesions of infected liver that showed traumatic and toxic effects produced by the migrating A. galli larvae. Except on the top, central veins were not obvious. Inflammatory tracts produced by migrating larvae were prominent. Large vein with portal sluggish consi...
S. Feroza1, A. G. Arijo1† and I. R. Zahid2
...i infectivity in broiler chicken. A total of eighteen broiler birds were randomly selected that were divided into three groups (A, B and C) with 6 birds in each group. The birds were then artificially infected with Ascaridia galli @ 2000 eggs/bird. Ethanolic extracts of papaya and neem were applied to Group B and C, respectively while Group A was left untreated that served as control. The fecal egg count (FEC) was conducted on weekly basis. The ...
S. Feroza 1, A. G. Arijo1†, F. M. Bilqees2* and M. S. Phulan1
...gical changes in broiler chicken was investigated. The postmortem examinations of broiler experimentally induced A. galli revealed gross pathological lesions of infected organs viz., small intestine, liver, lungs and heart. Hemorrhagic enteritis and acute fibrinous enteritis with clotted blood, hemorrhagic and reddish spots on intestinal wall, inflammation and necrotic patches with consolidation of intestinal contents were generally observed. Infected liver sh...
Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1
Sri Setyaningrum*, Dini Julia Sari Siregar, Tengku Gilang Pradana
...ut microflora of broiler chickens. The research used 144 one-day-old broiler chicks (44.33±1.09 g). The research was conducted with a completely randomized design. The treatments included of T0: diet + 0% CGLp (control), T1: diet + CGLp 1.0%, T2: diet + CGLp 1.5%, T3: diet + CGLp 2.0%, T4: diet + CGLp 2.5% and T5: diet + CGLp 3.0%. The treatment of CGLp was significantly increased (p <0.05) of body weight gain, live body weight, carcass percentage, m...
Sofiane Tamendjari1,2*, Farida Bouzebda Afri1,2, Lina Chaib1, Hebib Aggad3, Zoubir Bouzebda1,2
... of 90 samples of sheep, chicken and turkey meat were collected and analyzed. Modified Baird-Parker medium was used for enumeration and isolation of strains, free coagulase and thermonuclease tests were performed as well as other biochemical tests. Antibiotic susceptibility testing was performed to evaluate the resistance of S. aureus to antibiotics. Finally, statistical processing was performed using the STATISTICA 7 software (Statsoft, France). The percentag...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...nged to the W-36 Hy-Line chickens. The layers were randomly designated into five equal treatment groups (9 replicates × 6 hens per replicate for each treatment) according to the dietary inclusion levels of BSFL meal. The first group of birds were fed a basal diet of soybean-corn meals and served as a control (0% BSFL meal), while the remaining 4 groups were fed a basal diet in which the soybean-corn meals were partially replaced with 3%, 6%, 9%, and 12% ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
...ic efficiency of broiler chickens. The study included 360 unsexed Cobb500™ commercial broiler chicks aged 1 day and weighing 46±1.5 g. Starting from the 22nd to the 42nd d of age, the chicks were randomly distributed according to the dietary inclusion levels of MWM into six treatment groups (6 replicate pens per group × 10 chicks per replicate, considering the equal ratio of males to females in each replicate). The first group of birds was f...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...;) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector® was the best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV...
Fatma Salih Mohammed1*, Nasser Khedr1, Tahia Ahmed1, Liza S. Mohammed2
...adding EE-SBM to broiler chicken Ross® 308 feed formulations on carcass traits and histomorphological changes in the intestine. A total of 198 one-day-old Ross® 308 chickens were randomly distributed into three groups with different dietary treatments (66 birds per group). The dietary treatments were as follows: D1, control (soybean meal 46%); D2, D1 + EE-SBM (50 kg starter, 100 kg grower, and 200 kg finisher/ton); a...
A.E. Ismail and M. M. Mohamed
...), sheep manure (SM) and chicken manure (ChM) at three rates as organic substances, incombinination with urea as inorganic fertilizer, were tested for their action against root-knot nematode, Meloidogyne arenaria infesting sugar beet and plant growth, yield and total soluble sugars (TSS %) under new reclaimed sandy loam field. Results indicated that all treatments at their rates significantly (p≤ 0.05 and / or 0.01) reduced females, galls and egg-mass numbe...
Yusri Sapsuha1*, Nur Sjafani1, Suryati Tjokrodiningrat2
...e performance of broiler chickens is focused on maximizing performance parameters, i.e., fast chicken growth with low feed consumption, and with optimal health status. This study provides an in-depth review of the use of cloves in feed to improve broiler chicken production performance and health. In conclusion, cloves contain bioactive substances that can be used to improve broiler
Abdul Azis*, Afriani Afriani
...train Lohmann of broiler chicken. The experimental design was completely randomized, with 5 treatments and 4 replications. As a control, chickens were fed ad libitum (FR-0); chickens were given free access to feed during two periods of 2 hours from age 7-14 days (FR-1) and age 7-21 days (FR-2); chickens were given free access to feed during two peri...
SAFDAR ALI KHAN1, FARHEEN ANSARI1, SANWAL ASLAM2*, AMJAD HUSSAIN3, MUREED HUSSAIN1, MAJID MAHMOOD4, ALI MUHAMMAD4, RAHMAT ALI1 , ASAD ASLAM3 AND FATIMA ALI1
... present study, 150 farm chicken and 100 desi chicken were screened for the existence of parasitic helminthes. Among the desi chicken, 61 were reported positive for helminthes parasites by gross examination of gastrointestinal tract. In these 61 positive cases, 42 (68.85%) were found positive for nematodes, 6 (9.83%) for cestodes and remaining 13 (21.31%) had mixed infection; Ascaridia ...
Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4
Syeda Fakhra Waheed1, Asim Aslam1*, Muti-ur-Rehman Khan1, Kamran Ashraf2 and Beenish Zahid3
...ue samples from backyard chicken flocks presenting suspected symptoms were collected during February 2020 and March 2021 for genetic characterization, followed by phylogentic analysis. A total of 4 isolates were sequenced based on partial VP1 and hyper-variable region of the VP2 genes simultaneously. According to phylogenetic analysis, the study isolates genotype A3B3 were identified as predominant strains in country backyard poultry. Concerning the identity m...
Riko Noviadi, Dwi Desmiyeni Putri, Gusma Gama Maradon*, Agung Adi Candra, Nani Irwani, Gadis Apriani, Made Guntur Candra Adinata, I Made Krisnanda
Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2
...raits in 300 Thai native chickens were investigated. DNA was extracted from blood samples for genotyping using specific primers and restriction enzymes for each gene, and polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes (PCR-RFLP). Three genotypes were found for each gene as BB, Bb and bb for NPY; TT, TC and CC for DRD2 and II, ID and DD for VIP. Genotype frequencies of NPY (range 0.13-0.58), DRD2 (range 0.0...
Zulaikha Zainool Abidin1, Fahim Hafiy Idris2, Suriya Kumari Ramiah1, Elmutaz Atta Awad1, Zunita Zakaria3,4, Zulkifli Idrus1,2*
...bacillus spp. in broiler chickens. Thirty-five-day-old broiler chickens were subjected to either 0 h or 8 h of feed withdrawal, followed by 2 h or 4 h of road transportation. For each feed withdrawal-transportation subgroup, the birds were lairaged for 0 h or 3 h. Salmonella spp. and Campylobacter spp. were only detected in two and three birds, respectively. Because of the insufficient data, both types of bacteria were exclu...
Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1
... its potential growth in chicken breast and thigh meats at room temperature (25 °C) and refrigeration temperature (4 °C). The calibration curve showed a linear relationship between the turbidity measurement and bacterial cell count in TSB. Moreover, the coefficient of determination index (R2) was 0.8789 which indicates that the linear function was acceptable for reflecting the relationship between them. The growth kinetics of S. aureus obtained from Ba...
Hendy Musbawandi1, Mohammad H. Tamzil2*, Budi Indarsih2
...domized design using 180 chickens. All chickens were divided into three groups with six replicates each which were the first group was reared in a closed house, the second and third groups were reared in positive pressure and open houses. Each replicate, in each treatment, was reared in a different barn plot with the number of 10 chickens/m2 of the barn area. The results showed that feed c...
Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3
...mune response in broiler chickens. A total of 450 Cobb-500 unsexed DOCs were divided in response to the treatment into broilers fed a control diet (no additives), control supplemented with PB (100 g/100 kg feed), or control supplemented with AGP (Lincomycin 2.2%, 15 g/100 kg feed), having six replications in each treatment (25 birds per replication). Growth parameters were recorded. On days 22 and 34, HI antibody titer was determined against Newcastle disease ...
Nguyen Thi Kim Dong1*, Nguyen Van Thu2
...nd C. perfringens in the chicken excreta (p<0.05). It was concluded that the Guinea fowls supplemented SP and VE in concentrate basal diets improved growth rate, carcass performance as well as had better intestinal health and the treatment offered at 0.2% SP gave the highest results. ...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...n of the Kokok Balenggek chicken (KBC) as well as fertility, fertile period, and sex ratio as a result of artificial insemination with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into 500µl of Ringer’s lactate solution with various concentrations of spermatozoa, namely 50&...
Saad Zaghlou El-Damrawy1, Reda Ali Hassan2*, Ahmed Maher Bakhaty1, Adel M. Nasr2, El-Sayed A. Abu El-Hassan2
...effects of AF in broiler chicken. However, silymarin comparatively more efficiently ameliorate the effects induced by AFB1 than choline chloride. ...
Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz
...usually occur in broiler chicken. Use of AGP in Poultry feed has several negative implications involving public health concerns and trade restrictions. Bacillus amyloliquefaciens has been investigated as potential probiotic. The study involves the evaluation of competitive exclusion ability of B. amyloliquefaciens against S. Enteritidis and Cl. perfringens. A total of 180 commercial broiler chickens were obtained from local ...
Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2
...hawed buffen, chevon and chicken meat samples. Nutritive values of chilled, frozen and thawed buffen sample also decreased with increasing storage period....
Keatisak Soisuwan1, Hatairat Plaimast2, Atichat Thongnum3, Soranot Chotnipat4, Manatsanun Nopparatmaitree4*
...p up feeding) of broiler chicken significantly affected the economic return of broiler production. ...
Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1
...gy metabolism in broiler chicken. The study used 30 broiler male chickens with an average body weight of 1.5 kg at five weeks of age. This study used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder): A1 (brown seaweed), A2 (taro tuber), and A3 (tapioca flour), then factor B (dosage of pellet binder): B1 (1.5; 3.3; and...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...1 and Stx-2 genes in 160 chicken meat samples, which are randomly collected from different regions of Northern Punjab, Pakistan. Isolation of pathogen from meat samples were performed with the use of International Organization for standardization based microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. coli. Among them, 14 (8.75...
Rogia SA Gomez*, Said H Mbaga
... including raising local chicken (75.76%) and other animal species such as cattle, goats, and rabbits (41.7%). The average mortality rate recorded in these farms was 11.7%. Biosecurity Index Score ranged from 44-66% (mean 53.83 ±4.23). There was low adoption of biosecurity between the farm’s boundary and the poultry houses. The study concludes that in the Pwani region biosecurity was moderately applied, which shows that farmers in this region are ...
Md. Saiful Islam Siddiqui1*, Sharmin Akter2 and Mohd. Riajul Islam2
...ines for raising broiler chicken meat in Bangladesh therefore it is important to find out whether is any misuse of antibiotics. The goal of this study is to scrutinize the commonly used antibiotics, in broiler industries, to evaluate factors govern farmers “selection of antibiotics” and to assess the status of poultry farmers knowledge about antibiotic resistant (ABR). A cross-sectional survey was conducted over a period of around one year among 50...
Abdulaziz A. Alaqil
...on. Four hundred broiler chickens (Cobb500, 1-d-old) were randomly distributed in equal numbers in 40 battery cages (10 birds per cage). Every ten cages were placed in a room to arrange four experimental room groups identified as C, EC, PR, and PR+EC groups, respectively. From 22-42 d of age, the C and EC groups have received a basal diet with no PR supplements, whereas the PR and PR+EC groups have received PR at 1 g/kg of the basal diet. On day 36 of age, the...
Md Tariqul Islam1,5 *, Aditya Chowdhury Avi1,5, Md Saiful Bari2,5, Most Rebeka Sultana Swapna Khandoker3, Homaira Pervin Heema4, Goutam Buddha Das1
... ...
Aditya Chowdhury Avi1,4*, Md Tariqul Islam1,4, Md Saiful Bari2,4, Rebeka Sultana Swapna Khandoker3, Goutam Buddha Das1
Yaman Saad Fadhil
...0%, 5%, 10%, 15%) on the chicken meat stored for 12 days at 4°C temperature. The microbiological properties (total plate count, Coliform count, Staphylococcus aureus), and the sensory properties (colour, odour, Brittleness, and general appearance) were investigated. In general, increasing the propolis extract concentration in samples leads to a reduction in the growth rate of the bacterial population, and it continued till the last day of storage at 4oC. T...
Antonius Agung Wiono1, Maria Elizabeth Nana Wijayanti1, Dhika Yonika Primacitra1, Trioso Purnawarman2, Andriyanto3, Aulia Andi Mustika3, Amaq Fadholly3*
... productivity of broiler chicken.Materials and Methods:Broiler chicken were divided to six groups (normal control, negative control, heat stress treated vitamin C 200 g/ton and 400 g/ton, heat stress treated PhytoceeTM 200 g/ton and 400 g/ton). Its productivity was measured in different parameters including early – weekly - final body weight, feed consumption ratio (FCR). Each group were also evaluated through hematol...
Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1
...E. coli from slaughtered chicken of open poultry markets and to check that how much the Cephalosporins are resistance to ESBL E. coli in Abbottabad region. E. coli is isolated through General microbe’s culture methods and biochemical tests. The E. coli isolated is further tested for ESBL E. coli. The detection of ESBL E. coli is done through DDST and CDT. It was noted that out of 210 samples 08 samples were found negative (no growth of any bacteria found...
Nguyen Thi Anh Thu1*, Ly Thi Thu Lan1, Nguyen Thuy Linh1, Chau Cong Dang1, Nguyen Hoang Phuc1, Tran Dinh Tuan1, Nguyen Trong Ngu2
...s (SE) in native broiler chickens. Totally, 320 native chicks were divided into two experiments using a completely randomized design consisting of 4 treatments and 4 replicates of 10 chicks per experiment. In both experiments, BC was used at a dose of 109 PFU/mL, and SE was used at 108,2 CFU/mL. In the protective experiment, 1-day-old chicks were arranged in CD) control diet; BC+S) CD + 1 mL BC (gavaged at days 1, 2, 3, 4, 5) + 1 mL SE (gavaged at day 3); S) C...
Fuji Astuty Auza1*, Sri Purwanti2, Jasmal A. Syamsu2, Asmuddin Natsir2, Rusli Badaruddin1, Deki Zulkarnain1, La Ode Muh. Munadi1
... the proper nutrition of chickens, but their availability is often limited, and their cost is typically high. Therefore, this study aims to develop a ration formulation using black soldier fly (BSF) larvae flour to reduce the use of fish flour and improve performance, carcass quality, and nutrient digestibility of village chicken. A total of 140 one-day-old chicks (DOC) were randomly assigned to one of five treatments in a c...
Rizki Palupi*, Arfan Abrar, Fitri Nova Liya Lubis, Yuni Kurniati, Bianca Ikriza Octa Nadia Putri
...dehyde levels in broiler chicken blood. The study was conducted experimentally with a completely randomized design, which consisted of 5 treatments and 5 replications. The treatment was the use of a combination of cassava tuber and indigofera leaves meal (70%+30%; CCI) in the ration, namely: T0 (0% CCI in the ration), T1 (5% CCI in ration), T2 (10% CCI in ration), T3 (15% CCI in ration) and T4 (20% CCI in ration). The material used was 100 day-old broiler chi...
Abiyu Tadele 1,2*, Gebreyohannes Berhane2, Wondmeneh Esatu3
...nd egg quality traits of chicken are essential for consumers and chicken husbandry, helping them to anticipate yield, market, and exploitation of the genotypes in future breeding plans. The main ecotypes in Southwest Ethiopia are the indigenous naked-neck and normal-feathered chicken genotypes, and the exotic Tetra H, a dual-purpose genotype that was imported from Hungary. Data were collec...
Yoshio Hamano1*, Yasuji Kurimoto2
...tabolism. ...
Xiangjie Zhao1, Zheshi Kuang2, Jiyuan Yang2, Muhammad Bilal1 and Rongling Yang1*
...f Lingnan yellow broiler chickens. Lingnan yellow broilers were randomly categorized into 4 groups. The control group was fed with basal diet containing 3% fishmeal, and the treatment group 1, 2, and 3 were fed basal diet with 30%, 60%, and 100% fishmeal substituted with fermented silkworm pupae (FSP), respectively. Broilers were fed the treatment diets in phase 1 (1-21 d) and phase 2 (22-42 d) during the experimental period. Results showed a non-significant d...
Ertika Fitri Lisnanti1,4, Widya Paramita Lokapirnasari2*, Eka Pramyrtha Hestianah3, Mohammad Anam Al Arif2, Zein Ahmad Baihaqi4,5
...axillary vein of broiler chickens which is put in an EDTA tube. The parameters measured were: the number of leukocytes, erythrocytes, hemoglobin levels, hematocrit, platelets, lymphocytes, monocytes, granulocytes, types of leukocytes and blood glucose levels in broilers. The addition of different of Marsh fleabane leaves water extract in the feed had a significant effect on the levels of Hb, leukocytes and erythrocytes were within the normal range while all bl...
Pham Tan Nha*, Le Thu Thuy
...e study involved 180 Tre chickens, aged 6 weeks and weighing approximately 200±6.5 g/bird. Each experimental unit consisted of 10 birds, with a balanced distribution of sexes. The treatments included FG0.5, FG1.0, FG1.5, BG0.5, BG1.0, and BG1.5, where Fresh Garlic and Black Garlic were added to the basal diet at 0.5%, 1%, and 1.5% (DM) concentrations. The trial lasted for 8 weeks, covering the period from 6 to 13 weeks of age for the Tre
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
...eep infection of broiler chicken skin caused by many bacterial species, mainly Escherichia coli (E. coli) and/ or Staphylococcus aureus (S. aureus), causing severe economic losses in poultry. This study was done on 14-day old broiler Ross 308 chickens subcutaneous (s.c) injected with E. coli and/ or S. aureus to induce cellulitis. Clinical signs, mortality, pathological lesion, and growth performance were determined. Hematol...
Imbang Dwi Rahayu1, Ali Mahmud1*, Wahyu Widodo1, Adi Sutanto1, Apriliana Devi Anggraini1, Devi Dwi Siskawardani2, Wisnu Nurcahyo3, Tri Untari3
...s the quality of broiler chicken. This study aimed to determine the effects of giving herbs as feed additives through feed and drinking water on the biochemical parameters of broiler blood. In this study, 175 chickens were used and divided into seven levels of treatments; T0 (feed without herbs), T1 (feed + 1% herbs), T2 (Feed + 2% herbs), T3 (Feed + 3% herbs), T4 (drinking water +1% herbal), T5 (drinking water + 2% herbal),...
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...e II vaccines in broiler chickens. Eighty chickens were housed in four groups (A, B, C and D) of 20 birds per group. Group A has been received the experimentally prepared inactivated genotype VII NDV vaccine by day 9 old, subsequently primed and boostered with commercial live attenuated genotype VII vaccine at 7 and 21 days-of age. While group B was treated with commercial live and killed genotype II NDV vaccines at the same...
Xu Yun-Ming1*, Sun Zhi-Yuan1, Yang Jian-Bo1, Bian Rong-Rong2, Ren Hong-Lin3, Zhong Si-Yuan1, Cai Yu-Hong1, Peng Jing1 and Bao Hua-Xia1
...ficial polluted samples (chicken meat) can be detected by LAMP, when at least 45 min must be needed to enrich bacteria. Visual dye hydroxynaphthol (HNB) successfully used in LAMP assay was established for detection of B. cereus in meat....
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...tagious disease of young chickens, that results in bursal necrosis and severe damage of the immune system. IBDV remarkably persist to affect the poultry industry in Egypt. Hence this study was done to identify and characterize very virulent Infectious Bursal Disease virus in Egypt during the period 2019-2020. bursal samples were collected from 8 vaccinated broiler flocks exhibited high mortality rates and bursal lesions in al-El-Fayoum province, Egypt during 2...
Fahad A. Al-Abbasi1*, Abdullah Samer Habib1, Vikas Kumar2 and Firoz Anwar1
...ion in imported fish and chicken in Saudi Arabia and impact on food industry production workers. Sufficient samples (approx. 422) from imported fish and chicken were randomly collected and analyzed for total plate count (TPC), Staphylococcus aureus, Vibrio parahaemolyticus and Pseudomonas. Five healthy workers directly involved with processing, handling from various departments without any prior disease were selected for blo...
Mahmood Davoodi-Omam, Hossein Mansoori Yarahmadi*, Jafar Fakhraei and Mehdi Changizi
...ts, 6 replicates with 10 chickens in each replicate up to 42 days of age. The group containing Globacid (1 mL/L) and the restriction intensity of 10% had the highest significant difference compared to the control (p <0.05). The highest thymus weight was observed in the treatments with Globacid levels of 0.5 and 1 mL/L, respectively (p < 0.05). Also, the highest humoral immune response among the groups was seen in the group containing Globacid (1 mL/L) an...
Bayanda Mdoda*, Faith Machabi, Busisani Wiseman Lembede, Eliton Chivandi
...owth promoter in broiler chicken feed was evaluated by determining its effects on growth performance, feed intake and utilisation efficiency, gastrointestinal tract (GIT) organ, and GIT accessory organ macromorphometry, visceral fat mass and carcass yield. One hundred and twenty unsexed 1-day-old Cobb 500 broiler chicks (10 chicks per replicate with 3 replicates per diet) were randomly assigned to four dietary treatments where zingerone replaced zinc bacitraci...
Zeyad Kamal Imari
...sixty commercial broiler chickens were used in this study. The broiler chickens were randomly placed in 36 pens with 6 groups, each group contained 6 pens with 10 birds for each pen. Six experimental diets with different levels of calcium (Ca) were formulated to be as follows: 1.4% Ca, 1.2% Ca, 1% Ca, 0.8% Ca, 0.6% Ca and 0.4% Ca. The polynomial regression equations were used to estimate nutritional requirements of calcium....
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
...ence of AI antibodies in chickens and AI appraisal of poultry farmers’ knowledge, attitude and practice in Abuja Municipal Area Council, Nigeria. One hundred (100) blood samples collected randomly post slaughter from chickens in live bird markets were screened and characterized using Agar Gel Immuno Diffusion and Haemagglutination Inhibition Tests at the Avian Influenza laboratory, of the National Veterinary Research I...
Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3
...ofiling of Salmonella in chicken and ducks. A total of 125 feces samples (55 broilers, 45 layers and 25 ducks) were collected in district Hyderabad. Isolation was done according to ISO 6579 standard method. Identification of isolated bacteria was done using the Salmonella specific biochemical tests. Antimicrobial resistance patterns were determined using the disk diffusion method. Salmonella were detected in 22, 19 and 10 samples, showing prevalence of 40.0, 4...
Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8
... and on the last day two chickens from each replicate were slaughtered (a cumulatively eight chickens from each group). Samples from different regions (duodenum, jejunum, and ileum) of the small intestine were collected and kept in the fixatives. Slices from each part were stained with H and E stain to make it fit for microscopy. Picture were taken with different magnifications, i.e., 4X, 10X and 40X and histo-morphometry wa...
Raed Hussein Salih Rabee1, Yahya Sabah Abdulameer1, Walla Farhan Obed1, Noor R Abady1, Adnan Mansour Jasim1*, Firas Hussein Albawi1, Mohammed Jasim Jawad2, Ahmed Samir Abukhomra3
...evels of enrofloxacin in chicken meat after administering berberine (BBR). Escherichia coli (E. coli) was isolated from chickens suffering from diarrhea, and its isolation was confirmed by culturing it on MacConkey agar medium. In this study, a total of one hundred chicken chicks were reared, and on the tenth day of the experiment, they were divided into five equal groups:NCG: This group r...
Rusli Tonda1, Lili Zalizar1*, Wahyu Widodo1, Roy Hendroko Setyobudi1, David Hermawan1, Damat Damat1, Zane Vincēviča-Gaile2, Irum Iqrar3, Trias Agung Pakarti4,5, Shazma Anwar6 and Wirawan Wira7
... rice bran (T3). Broiler chickens aged 35 d with an average body weight of 1 870 g were involved – each treatment unit was of three, making a total of 27 chickens. Data gained from the experiment were run through one-way ANOVA, followed by an LSD test should any differences in the treatments occur. The result showed that consumption on T2 (59.89 g) was higher than on T1 (42.19 g) and T3 (9.22 g). As of feeding duration...
Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8
...) in each group. Broiler chickens feed with starter and finishing commercial corn based basal diet with different concentration of selenium powder. Basal diet (BD) was fed to Group A, which kept as negative control group and no dexamethasone (DE) was offered. Group B was considered as (+ve control group) fed with (BD+15mg DE/kg). The group C, was fed with (BD+0.2mg Se + 15mg DE/kg). Similarly, group D was fed with (BD+0.3mg Se+15mg DE). Group E, fed with (BD+0...
Promising Immunomodulation Potential of Ganoderma lucidium and Oregano as Mixture on Broiler Chicken
Fateh A. Badi*, Anwar A. G. Al-Kubati, Naif Al-Gabri
...anum vulgare on broilers chicken are not completely understood. In this study, four broiler chicken groups around 3 week age each group contain 10 chicken was divided randomly. First group was feed with (2g/ Kg feed) from Ganoderma lucidium (G group), second group was treated with ( 2g/Kg feed) Origanum vulgare (O Group) in a feed and the third group was treated with mixture of both Ganod...
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...ffect of yeast in sonali chicken based on possible ones on hematological parameters and histopathological changes. Sixty (60) sonali chickens of twenty-three days old were randomly divided into five groups and each group contained 12 chickens. All groups were supplied E. tenella orally except the T0 group and after 3 days T2 and T3 group was treated with yeast 1g/kg and 1.5g/kg feed for 14...
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...ffect of yeast in sonali chicken based on possible ones on hematological parameters and histopathological changes. Sixty (60) sonali chickens of twenty-three days old were randomly divided into five groups and each group contained 12 chickens. All groups were supplied E. tenella orally except the T0 group and after 3 days T2 and T3 group was treated with yeast 1g/kg and 1.5g/kg feed for 14...
Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3
...tage. ...
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