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Application of Phages to Control Escherichia coli Infections in Native Noi Chickens

Application of Phages to Control Escherichia coli Infections in Native Noi Chickens

Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*

1Department of Animal Sciences, College of Agriculture, Can Tho University, Can Tho City, Vietnam; 2Department of Veterinary Medicine, College of Agriculture, Can Tho University, Can Tho City, Vietnam; 3Department of Food Technology, Faculty of Chemical Engineering, Bio and Food Technolgy, Can Tho University of Technology, Can Tho City, Vietnam; 4Applied Biology Faculty, Tay Do University, Can Tho City, Vietnam; 5School of Agriculture and Aquaculture, Tra Vinh University, Tra Vinh City, Vietnam.

 
*Correspondence | Nguyen Trong Ngu, Department of Animal Sciences, College of Agriculture, Can Tho University, Can Tho City, Vietnam; Email: ntngu@ctu.edu.vn

ABSTRACT

This study assessed the efficacy of two phages, MHH6 and PR2, against Escherichia coli serotype O6 infected in chickens. A total of 420 broilers were randomly assigned to seven treatment groups. The negative control (NC) birds received no E. coli or phages, whereas the positive control (PC) birds were infected with E. coli only. The NC+MHH6 and NC+PR2 treatments received 109 pfu/ml of phages MHH6 or PR2, respectively; whereas the PC+MHH6, PC+PR2, and PC+MHH6PR2 groups were infected with 107.1 cfu/ml of E. coli strain O6 and treated with 109 pfu/ml of phages of MHH6, PR2, or both. E. coli infection was inoculated in two-day-old chicks, and phages were administered 24 hours later. The mortality rate of chickens in the PC+MHH6PR2 group was significantly lower (16.7%) than in the PC group (58.3%). The frequency of lesions and E. coli densities in the heart, liver, and spleen of chickens treated with MHH6 and PR2 phages were greatly decreased in infected chickens. After 98 days, the body weight of birds from the E. coli-infected groups treated with MHH6 and PR2 phages was lower than individuals in non-E. coli-infected groups (1,313-1,324 g/bird) but higher and significantly different from those in the PC group (1,172 g/bird). The majority of commercially important traits in chickens improved after phage treatment, proving that these phages are capable of controlling O6 E. coli infected in Noi chickens.
 
Keywords | Bacteriophage, Carcass characteristic, Chicken, E. coli, Growth performance
 

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Advances in Animal and Veterinary Sciences

September

Vol. 10, Iss. 9, Pages 1887-2089

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