James Ayukepi Ayukekbong1,2
E-mail | james.ayukekbong@microbio.gu.se
...nt studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to initiate the appropriate clinical care. Despite the endemicity of dengue in Nigeria, the disease is not a r...
Paulo Eduardo Brandao
... this treatment in human patients, the use of RNA interference (RNAi) for Post-transcriptional Gene Silencing (PTGS) has witnessed an increasing interest from those concerned with a cure for rabies, inspired on the success obtained for other viral diseases. This review is intended to present the current situation of RNAi against rabies, as well as insights into the future on the field....
Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2
... occurs in a minority of patients with viral hepatitis C, whereas chronic infection is established in 60 to 80% of all cases, 20 to 40% of which may evolve to cirrhosis and hepatocarcinoma. Genetic differences among infected hosts can determine the progression and outcome of the virus infection, causing different individuals to respond in different ways to the viral infection.
...
Josef D. Jarhult1, 2
...nza in otherwise healthy patients shortens influenza symptoms with approximately 24 hours, and increases nausea and vomiting by 3-5%. Whether oseltamivir treatment has an effect on the frequency of influenza complications or not remains controversial. Oseltamivir is still standard as prophylaxis, treatment of severely ill patients and patients with risk factors for severe influenza disease...
Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4
... evaluating stability in patients with various neurological defects. This test evaluates patients in 1 minute standing, but studies have shown that any stability test performed for less than five minutes is not clinically valuable. This study has evaluated stability with open and close eyes in normal subjects during five minutes standing in twenty healthy subjects. The subjects were asked to stand on a force plate for one an...
Ali Mohammadi, Mohammad Hossein Ebrahimi
...tment have been used for patients suffering from knee osteoarthritis (OA). Among them total knee artroplasty is being practised frequently. This method is suitable especially for elderly subjects with limited degree of physical activity. However, most of young subjects are not recommended to be treated with this method owing to high level of physical activities. KineSpring knee implant system was designed for young subjects with knee OA to ...
Seyed Mohsen Mirbod
...ve the performance of OA patients during standing and walking. Two subjects with knee osteoarthritis recruited in this research study. The gait performance of the subjects and their stability to stand was evaluated by the use of motion analysis system and a force plate (Kistler). The mediolateral excursion of the knee joint decreased while walking with the knee orthosis in contrast to normal walking. Moreover, the mediolateral moment applied on the knee j...
Keyvan Sharifmoradi1*, Nader Farahpour2
...force characteristics of patients with Parkinson’s disease (PD) and to compare with healthy age group. A total of 14 patients with PD and 16 normal elderly subjects with matched age and gender were included in the study. A motion analysis system (Vicon Motion Analysis System) and two Kistler force plates were used to measure forces applied on the legs. Data were evaluated using independent t-test with significan...
Tasnim Farasat*, Saima Sharif, Farkhanda Manzoor, Muneeza Zafar and Shagufta Naz
...newly diagnosed diabetic patients to assess the prevalence of diabetic retinopathy and to evaluate its relationship with potential risk factors of diabetes on their first visit to a local hospital. Among the newly diagnosed diabetic subjects 33% (n=66) were with retinopathy. Out of which 30% (n=18) were male and 70% (n=48) were female. High density lipoprotein (HDL), both systolic and diastolic blood pressure, cholesterol level and serum insulin were significa...
Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi
...mmuno-deficient Syndrome patients, several distinct and additional EBV-associated diseases may occur and some forms of malignancies. This study was designed to determine the incidence of EBV in Human immune-deficient virus (HIV)-infected individuals in Ogbomoso, Oyo State, Nigeria. Two hundred and seventy eight HIV-infected individuals were screened for EBV antibodies over a period of four months (August to November, 2014). The sera of individuals were subject...
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
... various genotypes in RA patients. A total of 400 individuals including age and gender matched healthy individuals (control group) and RA patients were genotyped. Detection of rs2070424 polymorphism was carried out using allele specific PCR based amplification strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical anal...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...healthy controls and 102 patients with hypercholesterolemia in a Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphisms was done by PCR-RFLP combined with gel electrophoresis. The genotype distribution in the population was found to be consistent with Hardy Weinberg equilibrium. Through logistic regression analysis, it was observed that, in Tamil population the CC and CT genotype carriers have higher odd...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...n and immune compromised patients. Abortion is one of the most serious outcomes in pregnant women. In this cross-sectional study, a total of 162 women of child bearing age were included from Rafha city of Saudi Arabia. Demographic data was recorded. Specific Toxoplasma gondii IgG and IgM were detected in serum by ELISA method. Toxoplasma gondii DNA was detected using PCR. Most women were in the 20-29 years age group (48%), followed by 30-39 years...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
...cally positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were females and 489 were male. Overall weighted prevalence of PCR positivity was 38% (268). Of the total 268 PCR positive subjects, 0.74%, 56.71%, 15.67% were positive for serotype 1, 2 and 3 respectively. Only 72 suspected patients had concurrent infection with serotype 2 and 3. Higher incidence o...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...y immune reaction in SLE patients. Pristane was injected in female BALB/c mice to induce the disease. After five months, mice in various groups were treated with prednisone and fed with gluten containing and standard diet for four weeks and applied procedure to detect minor changes in paw swelling, ANA autoantibodies, CCL11, C3c, glucose level and renal damage. We detected increased symptoms of arthritis and gastrointestinal tract involvement in gluten contain...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...establish vision in such patients but genetic researches have validated that, mutations in GJA8 are coherent source of lens opaqueness and inappropriate growth of fiber cells. In the present study, a novel G to C substitution (1104G>C) (pE368Q) was screened by PCR-SSCP in exon 2 of GJA8 and this tansversion altered exceedingly conserved glutamic acid to glutamine at site which was involved in coding of ASF1 like histone chaperone. Further presumption...
Nasar Khan1,2, Fahad Ali3,7, Sulaiman Bahadar5,*, Khalil ur Rahman3, Abdul Aziz3, Noor ul Akbar4, Muhammad Daud6, Azam Hayat5 and Mujaddad ur Rehman5
...659 HBsAg ELISA positive patients out of 16825 patients during July to October, 2013 were screened for HBV by real-time polymerase chain reaction. Out of 659, 522 (79.21%) were found positive for HBV, of which 318 (over all 4.4%) were male, while 341 (over all 3.55%) were female. The HBV PCR positivity rate was 80.18% (n=255) in males and 78.30% (n=267) in females patients. Among different...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...recruited a total of 100 patients diagnosed with OCD following Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) were selected from the same ancestry during August 2011 to January 2014fromSir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore. The demographic values for age, employment and marital status did not differ significantly among case and control groups. Exonic regions of BDNF gene were sequenced. In-silico a...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...1*12 in aero allergy patients is less as compared to healthy controls. HLA-DRB1*11 demonstrated significant association with aeroallergens and could have a protective role for allergy while HLA-DRB1*12 did not show any association with aeroallergen sensitization.
...
Saba Irshad1,*, Ayesha Ashfaq1, Ammara Muazzam1 and Abida Yasmeen2
...might be useful to treat patients as a considerably cheap herbal drug which can be prescribed to poor people efficiently at an affordable cost.
...
Omar S.O. Amer1,3,*, Mohamed I. Waly2,4 andSaeed A. Al-Zahrani1
...parasitic infections for patients visiting Prince Sultan Military Medical City, Riyadh, KSA from 2010 to 2014. Our retrospective study estimated a total 775 case out of 11110 case were infected with one or more intestinal parasites, with prevalence rate 6.98 %. The prevalence of intestinal parasitic infection during the period of study was as follows; Ascaris lumbricoides (n= 205, 1.8%), Giardia lamblia (n= 178, 1.6%), Entamoeba histolytica
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...nt>. We selected OCD patients (n=100) following the Diagnostic Statistical Manual-IV (DSM-IV) criteria and controls (n=120) from Sir Ganga Ram Hospital and Panjab Institute of Mental Health, Lahore from August 2011 to January 2014. During the sample collection the factors like age/period, employment status and marital status were considered after informed consent. We found one single base change G>C at c.745 resulting in a nonsynonymous change p.E249Q i...
Zahid Latif1,*, Kathrin Blasius2-4, Tufail Hussain Tahir7, Muhammad Nasim Khan1, Ghazanfar Ali5, Ansar Ahmed Abbasi6, Abdul Rauf1, Hao Hu8 and Angela M. Kaindl2,3,4
Sana Imran1*, Sarwat Jahan2 and Mohammad Sair3
...I). In 90 days study, 80 patients of mild and moderate psoriasis vulgaris were placed into A and B groups. The Betamethasone valerate treated patients were placed in group A while Calcipotriol treated patients were placed in group B; 40 patients in each group. The Psoriasis Area Severity Index (PASI criterion) was used to observe improvement in psoriatic...
Shumaila Ashfaq1, Liaqat Ali2*, Muhammad Ashraf Zia2, Rizwan Ahmad Khan2 and Mehtash Butt3
...logue Scalepain score in patients who developed PDPH after elective caesarean section under spinal anaesthesia who were given conventional treatment versus conventional treatment plus hydrocortisone.Double blind randomized control trial. Study conducted in department of anaesthesia and obstetrics of Allama Iqbal Medical College, Jinnah hospital (tertiary care) conducted from13th June 2014to 13th December 2014. Sample size was calculated 6...
Gut Microbe Analysis between Asthma Patients and Healthy Volunteers in Shaanxi Province, Xian, China
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*
...gut microbiota of asthma patients and to investigate the alteration in similarity and diversity of gut microbial composition with comparison of healthy controls. Fecal samples from fifteen patients and five healthy individuals were collected. PCR-DGGE, by using universal primers focusing V3 region of 16S rRNA gene, was performed for characterization of gut microbial composition. Sequencing of most dominant excised gel bands ...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...r genotyping of 200 MetS patients and 200 healthy controls was performed using a PCR-RFLP. Genotype and allele frequencies were calculated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to determine statistical significance. The frequency of 54Thr allele of FABP2 significantly associated with MetS risk in Pakistani population. Our results showed that frequency of the FABP2 54Thr allele wa...
Ghazala Saeed1, Rabia Wajid1* and Ayesha Yousaf Dar2
... delivery among eclampsiapatients.It is a descriptive case series conducted in Department of Obstetrics and Gynecology, Lady Willingdon Hospital, from December 2014 to June 2015. In our study, 62.86 % (n=88) patients were between18-25 years of age and 37.14 % (n=52) were between 26-35 years of age. The mean+SD was calculated as 25.12+4.53 years. Frequency ofvaginal delivery in patients wit...
Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2
...thmultiple schizophrenic patients and have identified some reasons for disease development. It is estimated from study that in Pakistan there is gender bias in schizophrenia unlike the worldwide data because more of males are affected in the selected family. For the males the age at onset was higher compared to females and the affected individuals were unmarried and unemployed. Parental consanguinity was another important determinant studied and was identified...
Maleeha Manzoor1*, Madiha Manzoor2, Qurat-ul-ain Ahmed3, Sobia Ahmed4, Shazia Manzoor5
...emographic characters of patients and their dietary history. Prevalence rate of anemia in the present study was found to be 78% (9.57±1.07), between age 19-40 years and it’s higher in 3rd trimester of their pregnancies. Additionally low educational status and poor dietary habits showed high risks of anemia among the pregnant women....
Tania Ahmed Shakoori1,*, Muhammad Saqib Saeed2, Asif Hanif2, Saima Mukhtar3 and Ayesha Ghauri4
...pulmonary disease (COPD) patients in a cross sectional analytical study design. For this purpose 137 patients were included in the study after obtaining necessary permissions from the ethical board of the hospital. COPD was diagnosed on the basis of clinical symptoms and a post bronchodilator values of FEV1/FVC<0.70 on spirometry. Frequent exacerbator’s were defined as patients ex...
Arooj Fatima1* and Ashfaq Ahmed2
...ric strength training in patients with rotator cuff (RC) tendinopathy. This single blinded randomized clinical trial was conducted at University Physical therapy and Rehabilitation clinic, Raiwind road, Lahore. The study included sample size of 50 subjects diagnosed with rotator cuff tendinopathy. Patients included in study were allocated randomly into; Group-A: This group was treated with routine physiotherapy treatment. Gr...
Muhammad Yahya Noori1*, Saharish Rizwan2, Zaheer Ali3 and Shaheen Sharafat4
...ttern of transmission in patients suffering from Multidrug resistant (MDR) Tuberculosis by assessing their contact information. Data were obtained and analyzed from 164 individuals suffering from MDR TB. Records were scanned to evaluate the presence of a close contact with known history of Tuberculosis. Tabulations and analysis were performed in Microsoft Excel® and online calculators. Analysis of contact data showed that only 36% of the
Samra Imran1,* and Afifa Tanweer2
...atus of Pakistani celiac patients. For this purpose 50 diagnosed celiac patients were selected from Shaikh Zayed Medical Complex and Mayo Hospital, Lahore. Nutritional assessment was carried out through anthropometric, biochemical and dietary evaluation of the participants. Results indicated that participants aged less than 18 years and those diagnosed within first year of life, had significantly healthier body dimensions. H...
Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
... current study total 794 patients were tested for suspected tuberculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis complex (MTBC) isolates were randomly selected for RAPD-PCR and PCR-restriction enzyme ...
Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar1
...that the mean age of the patients was 29.99±5.80 and the mean pain score before treatment in Letrozole group was 3.04±1.01 while in Danazol group was 5.05±1.02. Post-three months of treatments, mean pain score was 2.01±0.95 and 2.78±0.99 in Letrozole and Danazol groups, respectively. The mean decrease in pain score in Letrozole group was 1.02±1.09 (p-value=0.00) while in Danazol group; it was 3.06±1.23 (p-value=...
Nukhbat-Ullah Awan1*, Muhammad Sohail2, Zeeshan Sarwar3, Muhammad Mustahsen Bashir4, Muhammad Abbas Khokhar5 and Asad Aizaz Chatha6
...de II (moderate) tetanus patients.A total of fifty-six adults of both genders, diagnosed as grade II tetanus patients, were included. Clinical characteristics, mode of trauma, incubation period, vaccination status and investigations were recorded. All patients were managed in ICU and early tracheostomy was performed in addition to standardized medical treatment. A total of 86% males with a...
Mian Sajjad Ahmad1, Somia Iqtadar1, Sami Ullah Mumtaz1*, Zafar Niaz1, Iqra Waheed2 and Sajid Abaidullah1
...ucose tolerance in obese patients. This Cross Sectional study was carried out in North Medical Ward, King Edward Medical University/ Mayo Hospital Lahore for 6 months i.e. January to June 2014. Total 160 obese patients visiting outpatient department of North Medical Ward, Mayo Hospital Lahore for 6 months were included by non-probability purposive sampling. Patients aged 15-70 years of eit...
Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1
...ere measured in migraine patients and hypertensive patients with migraine. A blood sample of 3 cc was drawn from the median cubital vein after securing aseptic measures. The blood sample was allowed to clot for 30 minutes and serum was separated. The serum was then centrifuged andpro BNP levels were estimated by ELISA using Human pro BNP kit. Patients’ history and recorded blood pres...
Halima Shoukat1*, Hafiz Sheraz Arshad2, Faiza Sharif1, Arooj Fatima1 and Filza Shoukat1
Anza Zahid*, Muhammad Ali Ayub, Mujtaba Saeed, Nasir Pasha, Abdul Jabbar Dar, Huzaifa Javed, Aimen Shakeel, Amna Nasir, Ana Akbar, Zainab Tarrar and Ayesha Humayun
...n-compliance attitude of patients poses a great challenge to the success of therapy. Multiple factors influence compliance among diabetics and other chronic diseases and non-compliance results in avoidable consequences and complications. In order to determine the magnitude and factors of non-compliance to physicians’ advice in diabetics attending outpatients, a cross-sectional analytical study was conducted in outpatie...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...mated that about 100,000 patients are presently suffering from thalassaemia major, the severe form of the disorder. The patients of β-thalassaemia are dependent upon lifelong blood transfusion. Multiple transfusions expose them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemia major
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...t to November 2013) from patients at clinics, houses and pharmacies belonging to three Tehsils (Puran, Alpuri and Besham) of district Shangla. The Immuno-chromatographic (ICT) and enzyme linked Immunosorbant Assay (ELISA) techniques were applied for the presence of anti-HCV, while RT-PCR was used for active HCV infection. Our study reports an overall prevalence of HCV among 131 (14.55%), 103 (11.44%), and 94 (10.4%) by ICT, ELISA, and RT-PCR techniques in indi...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...losis at that stage. Two patients were reported with history of tuberculosis but were effectively treated before imprisonment. On age basis, 128/186 (68.88%) patients were of younger age (18-30 years) and 10 (5.37 %) were above 50 year. Illiteracy, unemployment (daily wages) and tobacco smoking were some of the main risk factors common in inmates. The trend of sharing blankets, towels and utensils were 158/186 (84.49%), 152/...
Muhammad Asim Khan1,*, Shahid Niaz Khan1, Sanaullah Khan2, Abdul Jabbar Khan3, Muhammad Adnan4, Nawab Ali5 and Ijaz Ali6
... -50 > (81.63%) years patients. The results also showed that highest infection (72.56%) was found in the month of August and (68.52%) in September, while the lowest prevalence of malaria infection was found in the months of December (7.93%) and in February (12.62%).
...
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5
...um magnesium levels. All patients were given Salbutamol inhalation and intravenous (I/V) Hydrocortisone. The study group was given 2gm of MgS04 intravenously in 20 minutes in a burette and Placebo group was given placebo (0.9% normal saline). Salbutamol inhalation was given at regular intervals and Spirometry was performed to determine lung functions.
Results: The two groups (study group and placebo-treated group) were compared fo...
Kaleem Ullah1, Zafar Niaz2, Sami Ullah Mumtaz3, Sajid Abaidullah4*
...ommonly reported in COPD patients.
Objective: This study was conducted to determine the presence of left ventricular diastolic dysfunction in patients of COPD.
Material and Methods: A cross sectional study was conducted in Mayo Hospital Lahore for one year. A total of 50 confirmed cases of COPD were included in the study. History of smoking was taken and subjects were examined for positi...
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
...ults: There were 190 patients of fibroadenoma (FA), 81 of fibrocystic disease (FCD), 64 of breast abscess (BA), 12 of granulomatous mastitis (GM), 7 of lipoma, 5 of phylloides tumor (PL), 4 of fibrosis (F), 2 of intraductal papilloma (IDP) and 3 of accessory breast with Fibrocystic (FC) changes. The relation between the ages and frequency of the different types of lesions was also analyzed to understand the association between predisposing factors and the ...
Masood Nizam Tabassum1*, Muhammad Ather Khan2, Saira Afzal3, Amir Gilani4, Abdul Wahab Gureja5, ShafaqTabassum6
...emographic parameters of patients of Tuberculosis at public sector health facilities Lahore, Pakistan.
Methods: A cross sectional study was conducted in outpatient Department of chest Medicine in Public sector health facilities Lahore, Pakistan from 1st January 2017 to 30th June 2017 after informed consent from Tuberculosis patients. After simple random sampling 1120
Muhammad Shaheer1*, Asad Aslam Khan2, Nasir Ahmed3, Tehseen Mahmood Mahju4, Ummarah Rasheed5
... visual loss in diabetic patients. However, the impact of medicinal treatment on the occurrence of macular edema remained elusive.
Objectives: This study was designed to determine the effect of intravitreal bevacizumab in patients of diabetic macular edema.
Methods: An interventional study was conducted on patients that underwent pre-injection and one-...
Naveed Akhtar1, Humaira Zareen2* and Rubina Sarmad3
...e a role model for their patients.
Objectives: This study was designed to access the dietary habits, nutritional status and their association in young adolescent female medical students.
Methodology: A cross sectional descriptive study was carried out in Medical College of Lahore. A total of 114 female students from 1st year and 2nd year were selected. Their socio-demographic characteristics, d...
Maaz Ahmad1*, Mussab Ahmad2 and Tehreem Munir3
...ic investigations in 200 patients diagnosed as cases of FD fulfilling Rome III criteria were recruited after taking written consent and randomly assigned to one of 2 treatment groups (CP extract group or placebo) in equal number. A 7 days medication free period was observed before the start of trial. Each patient received the treatment for 6 weeks. The primary outcome variable was the improvement in gastrointestinal symptom score (GIS) consisting of evaluation...
Taimoor Akram Khan1*, Anam Farooq2, Amna Farooq3, Muhammad Nasir1, Gohar Khan4 and Ali Akram Khan1
...PD register in which all patients that presented in the outpatient department of the facility during a period of 6 months from 1st January, 2017 to 30th June, 2017 were included and no patient was excluded.
Results: Out of total 2357 patients, 1024 (43.4%) were males and 1333 (56.6%) were females. Most of the patients 1057 (44.8%) belonged to the ag...
Farhat Ijaz1, Rana Khurram Aftab2*, Samia Jawed3
...here there were 50 obese patients with T2DM and 40 were non-obese Type 2 diabetic patients. TNF-α and serum insulin levels were determined using ELISA. Insulin resistance was calculated using HOMA-IR. Comparison between groups was performed using independent sample t-test. The P value ≤ 0.05 was considered statistically significant.
Results: Mean HOMA-IR and TNF-α values were significantly (...
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
...>Sputum samples from 125 patients were collected from confirmed pulmonary TB patients who were not responding to standard regimen of first line anti-tubercular treatment. Smears were stained by Ziehl-Neelsen method. All specimens were processed for culture and drug sensitivity by drug proportion method using Lowenstein Jensen medium as well as GeneXpert MTB/RIF assay.
Results: A total of 125 subjects were ...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...ples were collected from patients presenting to Jinnah Hospital, Lahore. Every sample was processed for bacterial culture. Bacterial identification was performed according to standard guidelines. Every gram-negative isolate was further processed for antimicrobial susceptibility testing by modified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resistant isolate were further processed f...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...d Methods: Sixty-six patients with newly diagnosed ALL were studied and patients on chemotherapy and T-ALL were excluded. The data was analyzed by SPSS20 for prognostically important parameters such as age, sex, hemoglobin level, WBC profile, platelet count, FAB type and immunophenotype. RNA extraction was performed and RT-PCR procedure was conducted to detect TEL-AML1 fusion oncogene.
Results: Out of ...
Muhammad Nusrullah*1, Muhammad Younus2 and Yasir Nasir3
...nd venous blood gases in patients presenting with chronic obstructive pulmonary disease.
Material and Methods: This cross sectional study was conducted at Department of Chest Medicine, Mayo Hospital, Lahore, Pakistan. After meeting the inclusion criteria, 100 patients were enrolled. Informed consent was taken and demographic information was obtained. Blood sample was obtained anaerobically from the radial ...
Faisal Masood*1, Ranjit Kumar Sah2 and Ahmad Humayun Sarfaraz3
...he mean age of total ten patients was 29.8± 3.20 years (25-35 years). We achieved union in 100% of the patient at mean time of 8.20 ± 0.92 weeks (7-10 weeks). There were 20% (n=2) cases with excellent and 80% (n=8) cases with good outcome according to Pattee and Thompson criteria. We had two cases (20%) with pin-tract infection that were managed with dressing and oral antibiotics according to culture and sensitivity. There were no loss of reducti...
Naveed Rashid*1, Muhammad Aamer2, Uzma Malik3, Nimra Arif4, Muhammad Waqar Akram5 and Ayesha Irshad6
...age renal disease (ESRD) patients who are on maintenance hemodialysis.
Methods: This comparative cross sectional study with quantitative approach was carried out in hemodialysis unit of Shalamar Institute of Health Sciences, Lahore for the month of July, 2017. All patients with ESRD, on maintenance hemodialysis through an AVF access for at least last 30 days, were included in the study. Each patient ...
Mumtaz Akhtar, Iffat Naheed, Khadija Waheed* and Amna Khanum
...were used. A total of 220patients undergoing normal vaginal delivery at term after administration of per rectal misoprostol were enrolled with 95% confidence interval (C.I). The error margin was 5% and the prevalence of postpartum haemorrhage was 16.5%. Data was entered and analyzed through SPSS 17. In this study, the mean age of study participants was found to be 29.89±5.89 years while the mean gestational age was 38.86±1.38 weeks. Postpartum ha...
Sana Rafique1, Hina Saqib1, Khushi Muhammad2, Nazia Akbar2, Abdul Rauf3, Muzafar Shah4,*
...ansehra, Pakistan, where patients were screened for dengue virus non-structural protein using Rapid Diagnostic kit for Dengue virus (DENV) i.e. SD BIOLINE Dengue IgG/IgM. Data was obtained from patient’s record, filled forms and through questionnaire. Total 1332 blood samples were collected from 3 tehsils of District Mansehra. Out of which, 725 were found positive for dengue fever infection. Out of 725 positive cases, 410 (56.5%) were males and 31...
Muhammad Sarfraz*, Shahida Hasnain
...zed and non-hospitalized patients from July 2013 to June 2016. API 20E system has been used to identify K. pneumoniae and E. coli; preliminary screening for AmpC beta-lactamase production was done by cefoxitin disc followed by inhibitor based confirmatory method. Antibiogram was performed following CLSI guidelines 2013. Of 11,725 clinical specimens, 29 % were culture-positive. 80% K. pneumoniae and 70% E. coli showed resistan...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
... from tracheal aspirates patients suffering from chronic and acute lung infections admitted to a local hospital in Lahore. Susceptibility of 134 isolates of P. aeruginosa was tested against selected antibiotics (meropenem, imipenem, piperacillin, amoxicillin, amikacin, gentamicin, tobramycin, kanamycin, clarithromycin, clarithromycin, cefepime, cefixime, levofloxacin, and ciprofloxacin), Lactobacillus strains and metal nanoparticles (copper, ferr...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...c strains in sore throat patients. However, the intraspecific association was noticed among these genes. Coa and spa polymorphism and association analysis indicated that spa negative isolates possess Coa of 1200 and 900bp, whereas spa positive isolates contain Coa of 650bp and 750bp. The most prevalent genes, spa, CPs8 and sea may be considered as molecular targets in designing treatment and contro...
Iqra Arooj1,* and Shahida Hasnain2
... study of which 435 were patients visiting hospitals of Lahore and Gujranwala districts. All the subjects were genotyped by allele-specific PCR. It was observed that the loci 6q25.1/rs6922269 and 9p21.3/rs1333049 were significantly associated with risk of CHD in this population (P=0.001 and 0.012, respectively).
...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ality among hospitalized patients across the globe. Currently, β-lactam ring containing antibiotics predominantly carbapenems, are considered as last treatment option against multi-drug resistance GNR infections. However, the emergence of carbapenemases particularly metallo β-lactamases (MBLs) in bacteria have severely mitigated the efficiency of carbapenems. MBLs producing gram-negative bacteria have been reported from various hospital settings, wor...
Shabana*, Saleem Ullah Shahid and Shahida Hasnain
...wed that Pakistani obese patients are hyperlipidemic except for HDL, which is low in obesity. The anthropometric traits were significantly different between dyslipidemic obese subjects and nondyslipidemic non obese subjects. In conclusion, obesity is accompanied by a dyslipidemic profile and increased weight and/or BMI. The progression of clinical forms of obesity can be assessed by the strong predicting indices, including weight, BMI, low HDL and high TC.
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
... to May, 2016 on and MCD patients were diagnosed by visiting different hospitals like, General Hospital, Al-Ehsan welfare Hospital, Mughal eye and Mayo Hospital with the help of ophthalmologist. The main purpose of such type of research was directed to find the prevalence of Macular corneal dystrophy in the families of Lahore. The techniques used for the diagnosis of MCD were visual Acuity test by Snellen chart, phoropter, slit lamp biomicroscopy, topography, ...
Asima Bano1, Hafiz Muhammad Tahir2, Hira Sherawat3, Muhammad Mutlib4, Muhammad Arshad5, Muhammad Akram Qazi6, Sajida Naseem5, Rabia Ishaq1, Iram Liaqat2
... plasma samples from the patients suffering from breast cancer and age matched healthy individuals (control) were collected. The results revealed decline in the level of these enzymes in breast cancer patients as compared to healthy individuals because of this decline the overall level of protein was also found to be decline. It is concluded from the study that these enzymes can be used as biomarker in the diagnosis of breas...
Saba Irshad*, Aruba Muhammad, Ammara Muazzam, Farah Sarfraz Anmol and Rehman Shahzad
...β-Thalassemia Major patients for Xmn1 Polymorphism, accountable for increased Fetal Hemoglobin, an important ameliorating factor in minimizing disease severity. PCR-RFLP was employed for securitizing Xmn1 polymorphism among thalassemia (Major) patients. Out of total 206 screened patients, sole Xmn1 homozygous (+/+) and heterozygous (-/+) case was reported with a band size of 418 bp, 2...
Mian Azhar Ahmad1†*, Nawab Mohammad Khan2, Asmatullah3
Anatomical variations of hepatobiliary triangle in patients operated laparoscopically for gallbladder diseases from Lahore and Sahiwal
...of Lahore and Sahiwal on patients who presented with symptomatic gallstones during the period 2011 to 2015. All the patients irrespective of their age, sex, past history of acute cholecystitis and obesity were subjected to Laparoscopic cholecystectomy. Patients’ age was between 18-70 years, gender ratio was 1:3.5 with female dominance, mean operative time 99.6 minutes and
Riffat Mehboob1*, Sami Ullah Mumtaz2, Zoya Manzoor3, Sajid Abaidullah2, Fridoon Jawad Ahmad1
Deranged biochemical and hematological profile of septicemia patients in Mayo hospital, Lahore
...epticemia. 101 confirmed patients of septicemia from a tertiary care hospital in Lahore were included. Liver and renal function tests were performed for all patients. Patients were divided into 3 age groups on the basis of age: 30-50, 51-70 and 71-90 years. In Liver function Tests, ALT (37.62%), AST (50.49%) and ALP (99%) were elevated, Bilirubin was normal in majority of
Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*
Risk factors for multiple sclerosis in Pakistani population- A crosssectional study
... 2014. The first hundred patients who came for
reporting MRI of the brain and had positive findings of MS were retrospectively evaluated. Mean age of the patients
was 43.07±13.88 years, with youngest patient as 18 years old and eldest as 65 years. Our study depicted 19% cases
of MS were smokers. 8% of the patients had positive history for viral infections. 8 % o...
reporting MRI of the brain and had positive findings of MS were retrospectively evaluated. Mean age of the patients
was 43.07±13.88 years, with youngest patient as 18 years old and eldest as 65 years. Our study depicted 19% cases
of MS were smokers. 8% of the patients had positive history for viral infections. 8 % o...
Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3
Mutation analysis of RING1 domain of Parkin in early onset of Parkinson’s disease in Pakistani patients-a pilot study
...ed in a sample set of 30 patients (selected from different areas of Punjab, Pakistan) to find out
any Single Nucleotide Polymorphism (SNP).No SNP was detected in RING1 domain that could be related to the
disease. The data suggests that no genetic predisposition in RING1 domain may be responsible for the occurrence of
disease in local population. It may be due to genetic changes in any other part of the gene or due to other
environme...
any Single Nucleotide Polymorphism (SNP).No SNP was detected in RING1 domain that could be related to the
disease. The data suggests that no genetic predisposition in RING1 domain may be responsible for the occurrence of
disease in local population. It may be due to genetic changes in any other part of the gene or due to other
environme...
Sobia Faisal1*,Qurat-ul-Ain Ahmad2, Maleeha Manzoor3, Madiha Manzoor4, Faisal Rifaq5
Socio-psychological factors related to tuberculosis diagnostic test seeking behavior in Pakistan
...ior of tuberculosis (TB) patients globally,
however, the need to understand why these factors affect the health seeking
behavior of TB patients in the light of behavioral theories in still unmet. This study
evaluates the role of social and psychological factors affecting TB diagnostic test
seeking behavior of patients in low socioeconomic country like Pakistan i...
however, the need to understand why these factors affect the health seeking
behavior of TB patients in the light of behavioral theories in still unmet. This study
evaluates the role of social and psychological factors affecting TB diagnostic test
seeking behavior of patients in low socioeconomic country like Pakistan i...
Ajmal Khan* and Rehan Ullah
...ogic agents in suspected patients reported to various laboratories of Agency Head Quarter Hospital Khar, Bajaur Agency. In this study, a total number of 9720 suspected individuals were examined; among which 1273 (13.1%) were found to be positive for malaria infection with different Plasmodium species in their blood smears. Male population accounts for higher prevalence rate (14.59%) than female (11.24%). The infection was more prevalent in November (21....
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...s, Lahore, Pakistan. The patients showed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure and infiltrate the entire eye ball. Diagnosis was made based upon the clinical and histopathological examination of the tumor sample. In all cases, tumor resection was performed followed by cauterization of th...
Muhammad Saqib Shahzad1, Amina Arif1,*,Saeeda Kalsoom2, Javed Iqbal3, Shahnam Shafique2, Muhammad Shahid Nadeem4 and Rafique Ahmed5
... hepatitis C virus (HCV) patients. Overall, 590 HCV patients were evaluated pre and post combined drug therapy during the period 2015-2017. Distribution of HCV genotypes, age groups, viral load and alanine aminotransferase (ALT) values were recorded and analyzed for rapid virologic response (RVR), non-rapid virologic response (NRVR), sustained virologic response (SVR) and non-sustained virologic response (NSVR). Among the ev...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...NA in neutrophils of the patients with chronic renal failure (CRF). It was used to dynamically observe of Hemodialysis on the concentration of the CXCL8 in serum and the levels of CXCL8 mRNA in neutrophils were respectively measured by ELISA and PCR. The house keeping gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...112 samples including 87 patients and 25 normal healthy controls. The geNorm algorithm exhibited circulating mir-16-5p with the highest expression stability demonstrated by geNorm M value of 0.418. The comparative delta Cq method showed no significant difference in Cq values of ALL patients and healthy controls suggesting that mir-16-5p is the most stable normalizer for miRNA expression profiling assays in ALL. The results r...
Nasira Khatoon1, Aly Khan2,*, S.S. Shahid3, Z.S. Saify4, M. Arshad Azmi1 and Wali Khan5
...s life threatening. Male patients visiting Baqai Medical University were grouped into five categories based on their age at the first visit. The diagnosis was based on detailed history and clinical examination which included scraping of papillae or burrow to confirm the diagnosis. To test the incidence in various age groups and body parts a chi-square (χ²) test of association (R × C contingency test) was performed. The association of body...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched controls by allelic discrimination. Of 99 patients with CHD, 26 had DS. MTHFR rs1801133 and rs1801131genotypes and alleles were not significantly different between controls and CHD
Amna Younus1,3, Mariam Faiz2 and Abida Yasmeen1,*
... Pakistani breast cancer patients. Newly diagnosed 300 breast cancer women from different areas of Punjab visiting Institute of Nuclear Medicine and Oncology Lahore were included in this study. Different clinico-pathological features were described. Vitamin D levels were determined and VDR FokI and TaqI SNPs were analyzed. Among different clinico-pathological features studied in breast cancer women, more prevalent were invasive ductal carcinoma (...
Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...d from 37 TNBC diagnosed patients and 34 age-matched non-TNBC controls to investigate the expression of miRNA panel. Plasma was isolated by standard protocol of centrifugation and then isolation of total RNA including miRNA was done. About 100ng of isolated miRNA was converted to cDNA after addition of poly (A) tailing and then expression profiling was done by using real time-qPCR technique in which miR-specific DNA primers were used to increase sensitivity of...
Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
...V, identified in two CRC patients, which may account for transformative capacity by biochemical and signalling readouts in these patients. Dynamical implications and functional impact of variants were determined by in silico analysis and molecular docking of variants with GTP. MutationTaster was used for functional analysis of genetic variants and three-dimensional structure of mutant proteins were built by Swiss-Model and w...
Abid Hussain1,2, Farida Ahmed3, Shyamali C. Dharmage4, Shafiq ur Rehman1, Muhammad Shahbaz Aslam5 and Zaigham Abbas1,*
...esigned to study allergy patients attending Allergy Centre, National Institute of Health, Islamabad. All enrolled patients were screened for set of allergens using skin prick test (SPT). Based on results of SPT, out of total 50550 allergen sensitized patients, 89.28% had aero-allergen sensitization while remaining 10.72% had food sensitization. Significantly higher prevalence of both aero-...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
...1/2 in breast cancer patients in Balochistan. Blood samples of 100 subjects including 50 breast cancer cases and 50 normal subjects were used to determine genetic variants in BRCA1 and BRCA2. Nine variants were identified in BRCA1 and four were identified in BRCA2. In case of BRCA1, six missense substitutions (p.Asp343Tyr, p.Gly393Asp, p.Ser561Phe, p.Ser616Phe, p.Pro871Leu and p.Ser1613Gly) two frameshift (p.Ser423fs and ...
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
...A gene in non smoker patients of non-small cell lung cancer (NSCLC). Somatic mutational analysis was done by QMC-PCR following direct sequencing with sangers’ method. Only one sample detected p.E545K mutation at c.1633G>A. Another most common mutation was observed in PIK3CA exon 20 which leads to change A>G at codon 1047. This transition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K r...
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian, Jonathan Hartanto Tan and Zheng Shusen*
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2
...e were more seropositive patients than RNA positive ones. Serotype-2 remained the main causative serotype of the outbreak of dengue viral infection, started in the commencement of year 2013 and continues to infect Pakistani population till now.
...
Aqsa Javaid and Nageen Hussain*
.../AIDS. A total of 25 HIV patients were chosen from the Institute of Public Health on the basis of confirm HIV infection and 25 healthy controls as well. First genomic DNA was extracted from the peripheral blood and then amplified by using specific designed primers. Gradient PCR was performed and the product length was 197 bps which was further analyzed on 1% agarose gel. Sequencing was done through genetic analyzer (3500 ABI). No mutation was observed in FO...
Hafiz Suboor ul Hassan and Saira Malik*
...G genotype, while 43.58% patients showed heterozygous GA genotype. Thus, different variants of genotype -13910T/C and -22018G/A of the individuals were found to be associated with the development of lactose intolerance in patients of the studied population. Milk intake status and gastrointestinal disease are among the other factors that may influence the onset of lactose intolerance in patients
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian and Zheng Shusen*
...n: justify;">Hepatitis B patients are required to be managed to avoid any additional infection that could come from surgical procedures or through unhygienic conditions. In this case report, we demonstrate the management, care and complication of liver transplantation in patients with pre-established hepatitis B infections. Information gained through this study would be helpful to have better management and life style in the...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
...nfirmed for HCV positive patients. Multiplex RT-PCR data showed that 75 (78%) patients had single HCV genotype infection (3a); three with mixed HCV infection (3a/2a, 3a/3b and 3a/1a) while 18 samples had no HCV signal. The current study demonstrated the high prevalence of HCV in our locality with the most prevalent HCV genotype 3a.
...
Hafiz Muhammad Arsalan1, Maria Altaf1, Zeemal Seemab Amin1, Muhammad Khalil Ahmad Khan2, Anum Shahzadi1, Hina Mudasser1, Iqra Maqsood1, Nazia Gulshan1, Saira Naseem3
... in Rheumatoid Arthritis patients. 60 clinically diagnosed Rheumatoid Arthritis (RA) patients and 50 healthy persons were included in this study. 5 ml Blood was drawn and serum was separated. Anti-Oxidant Biomarkers, Serum micronutrients, serum Electrolyte balance was measured through spectrophotometric procedure. Serum MDA level jump high in patients (14.19) as compared to normal subjects...
Tanweer Kumar1, Sahib Zada2,3,Muhammad Irfan4,*, Huma Batool5 and Wasim Sajjad2,6
... and the risk factors in patients with HBV. A total of 4,758 blood samples from the mixed population were collected including 2382 male 2376 females. All the samples were subjected to hepatitis B surface antigen Immuno-chromatographic test (ICT), HBsAg enzyme-linked immunosorbent assay and by Reverse transcription polymerase chain reaction for the existence of antibodies against HBV. It was observed that 307 (12.8%) out of the 2382 individuals harbored antibod...
Asima Rani1,*, Syed Kashif Nawaz2 and Muhammad Arshad3
...d samples of 228 malaria patients and 226 healthy controls were selected from the local population. Malarial samples were divided in to complicated malaria (N=89) and mild malaria (N=139) groups according to WHO criteria. Malarial groups were further divided into P. vivax and P. falciparum groups based on the Plasmodium species responsible for the infection. Allele specific PCR was employed for the amplification of rs8177400 polymorphism. ...
Sajida Rasool1, Saba Irshad1*, Neelam Saba1, Mehak Fiaz1, Muhammad Sajid Hussain2, MuhammadWajid Hussain3 and Peter Nürnberg2
Bamigboye Olutoyin Omolara1, Fapohunda Funmilayo Omotoyosi1, Oladipo Elijah Kolawole1,2, Oni Mattthew Olujenyo1, Ajibade Oluwatosin Akinola1, Kaka Mary Oluwatosin1*
...cination in HIV-infected patients is encouraged with careful monitoring for liver function, particularly those under ART. ...
Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5
...pproximately 350 million patients of hepatitis B and 170 million patients of Hepatitis C are present worldwide according to WHO. Many risk factors are involved in the transmission of theses deadly viral infections but blood transfusion in Beta thalassemic patients is working with two faces, one as remedy and the other is key risk factor in the spread of silent killers. Thalassemia
Chuntao Li1, Hua Liu2, Jianqing Zhang1*, Jiaqiang Zhang1, Luming Dai1, Zhihuan Zhao1,Lizhou Fang1, Ling Liu1, Jingkui Shu1 and Jiagang Feng1
... blood samples from COPD patients (n=3) and healthy controls (n=3) with smoking history by human microarray analysis. Then, we selected lncRNAs and their associated mRNAs which were both differential expression in COPD vs healthy smokers, for quantitative reverse transcription PCR (qRT-PCR). A total of 3,359 lncRNAs revealed by microarray analysishave been differentially expressed between the two groups, 1,995 lncRNAs identified to be up-regulated in COPD, whe...
Hong-mei Gao, Wen-long Su, Gui-bin Tao, Han-yang Li, Wen-zheng Cao and Zhi-dong Qiu*
...ll levels drop in cancer patients treated with chemotherapy. Geniposide can reduce the adverse radiation damage of bloosd tissue after high dose irradiation.We found that geniposide can recovery the red blood count after chemotherapy medications (5-Fluorouracil, 5-FU) treatment mice. The mechanism of action of genistein on erythrocyte production is not clear. This study suggests that Geniposide promotes red blood cell production. We demonstrated that genistein...
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*
...plication in AD-affected patients.
...
Ahsan Numan1,4, Khadija Irfan Khawaja2, Abdul Basit Qureshi3, Muhammad Shahbaz Yousaf4, Imtiaz Rabbani4, Hafsa Zaneb5 and Habib Rehman4*
...jects were screened. The patients with DSP were older (p < 0.05), had longer duration of DMT2 (p<0.01), elevated systolic blood pressure (p<0.05), blood sugar (p < 0.05), HbA1c (p<0.001), cholesterol (p<0.01), LDL (p<0.001), triglycerides (p<0.05) and more obese (p<0.01) when compared with the diabetic patients without clinical DSP. Occurrence of fatty liver was more (p<0.01) frequent...
Saima Sharif*, Farkhanda Manzoor, Tasnim Farasat, Shaugfta Naz and Raheela Tabasum
...inine of ischemic stroke patients was found to be 2.41 mg/dl compared to 0.93mg/dl in controls. The blood urea in ischemic stroke group was 67.3 mg/dl in contrast to 34.6 mg/dl in controls. The eGFR in ischemic stroke patients was calculated to be 54.62ml/min/1.73m2, compared to 85.90 ml/min/1.73m2 for controls. Prevalence of eGFR <60 ml/min/1.73m2 in patients with stroke was 63%, signi...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...DR1 expression levels in patients of the positive-group survival curves were lower than those in patients of the negative-group curves. High MDR1 expression is associated with clinicopathological features in malignant tumor patients. The detection of MDR1 expression combined with tumor markers can improve the sensitivity and specificity of predicting postoperative recurrence (especially br...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...cted from all age groups patients visiting hospital labs and District Head Quarter Laboratory who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found to be infected with malaria parasite. Data was also collected sfrom other labs of District Swat which accumulated to a ...
Sana Khan1, Raheela Taj1, Noor Rehman2, Asad Ullah3, Imad Khan3 and Sadeeq ur Rahman3,*
...zed and non-hospitalized patients of Khyber Teaching Hospital (KTH), Peshawar, and processed by for isolation of C. freundii followed by phenotypic detection of β-lactamases and antimicrobial susceptibility profile. Our results indicated that a total of 130(4.40%;130/2950) samples were positive for C. freundii comprised of urine 58.46%, pus 33.85% and wounds 7.69%, respectively. More samples from females (n=70) than males (n=60) with ...
Khayyam1*, Muhammad Zahid1, Naiz Ali2 and Qadeem Khan3
...y two autoimmune thyroid patients 50 were Hashimoto’s thyroiditis patients (HT) (30 female, mean age=34±1.43 years, 20 males mean age =38.35±1.17 years) and control group included 35 individuals (20 females mean age=31.65±1.77years, 15 males mean age=37.93±2.561 years). The 32 Grave’s disease (GD) patients (24 females mean age=34.96± 2.18 year...
Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2
... apart from hemodialysis patients, unprotected sexual intercourse, blood transfusion and vertical transmission. Therefore, there is a need for government commitment for HCV elimination program through making the DAAs available and its affordability to general population to curb the catastrophic cost of managing the epidemic. However, the diagnosis of HCV infection is faced with several challenges, therefore there is a demand for development of easy to use and ...
Gulzar Mehmood1*, M. Zahid Khan1, Haseeb Ur Rahman1, and Sohail Abbas2
AN EFFICIENT AND SECURE SESSION KEY ESTABLISHMENT SCHEME FOR HEALTH-CARE APPLICATIONS IN WIRELESS BODY AREA NETWORKS
...ications is security and patients’ information privacy. In such applications ensuring human
vital sign privacy and security is very important. To address this important issue, in this paper we propose a novel
secure session key establishment scheme for WBAN in health-care domain to ensure security and privacy of vital
sign related to human body. In the proposed scheme, session key is established for a specific period of time in order...
vital sign privacy and security is very important. To address this important issue, in this paper we propose a novel
secure session key establishment scheme for WBAN in health-care domain to ensure security and privacy of vital
sign related to human body. In the proposed scheme, session key is established for a specific period of time in order...
Sitara Nawaz1, Shamaila Irum1, Haroon Ahmed2, Shazia Shamas1, Sadia Roshan1, Muhammad Ather Rafi4, Irfan Ullah5*, Gulnaz Parveen3*
...nical information of 124 patients was recorded. Observable factors of prevalence include age group, gender, financial status, clinical symptoms, and travel history. The sandflies also collected and identified. Out of 350 sandflies two genera Phlebotomus and Sergentomyia were reported, P. segenti was most abundant specie and a strong positive linear correlation exists between sandflies collected and the patients
Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*
Hepatitis E Virus and Zoonosis
...uppressed and transplant patients where prolonged virus shedding has been documented. The conventional diagnosis is established by detection of HEV antigen or anti-HEV IgM in acute sera. However, the detection of HEV RNA is important to establish the diagnosis in immunosuppressed patients. In transplant patients, decrease in immunosuppression is usually done, however ribavirin therapy has ...
Muhammad Khan1, Tehmina Ameer Khan1, Aziz Ud Din2, Muhammad Fiaz Khan1, Irfan Ullah3,*, Kalim Ullah4, Sadia Tabassum1,*
... previously reported, in patients of German and French population. For the first time, the mt-1888A>G and mt-1811A>G mutation in MT-RNR2 gene have been observed in patients of HCM in the Pakistani population and even not found in the control group of the same families. No mutation in mt-DNA encoded tRNAIle, and tRNALeu genes were observed, the mutations detected in mt-DNA encoded 16S-rRNA g...
Yan Wen1, Liu Lian2, Zhuang Xun3, Murong Jiaying4, Cui Shuyi1, He Wanwen1, Mai Guanghuai5, Huang Wenzhu1, Chen Gang1,* and Wang Zhijun1,*
...rden setting for elderly patients affected with chronic obstructive pulmonary disease (COPD). The elderly patients suffering from COPD were randomly divided into an observation group (n=30) and a treatment group (n=30). Accordingly, the patients in the observation group were given a medication-assisted treatment plan for stable COPD based on established guidelines and indoor rehabilitation...
Muhammad Zahid Mengal1, Hamida Ali2, Raheela Asmat3, Muhammad Naeem1,4, Ferhat Abbas1, Abdul Samad1, Mohammad Zahid Mustafa1, Jannat Raza2 and Tauseef M. Asmat1*
...lected from suspected TB patients at Fatima Jinnah General and Chest Hospital Quetta, Pakistan between January and August 2017. These specimens were analyzed by GeneXpert® MTB/RIF assay. The data was statistically analyzed using SPSS software. Out of 2300 clinical specimens, M. tuberculosis was positive in in 899 (39.1%) cases by GeneXpert® MTB/RIF assay [positive respiratory cases 42.9% (871/2032) and non-respiratory 10.4%...
Rida Younas1*, Tamseela Mumtaz1, Nabeela Roohi2 and Muhammad Amir Iqbal3
...ere and very severe COPD patients and established their relationship with lung obstruction. Samples were collected between April and August 2017, total 35 COPD patients with lung obstruction (FEV1/FVC< 70) were enrolled as well as age and sex matched healthy subjects were also recruited as control group. Expression of IL-6 and IL-8 in serum samples were evaluated by Enzyme-linked immunosorbent assay. Correlation between s...
Yasir Sharif1, Saba Irshad1*, Ammara Muazzam1, Muhammad Hamza Tariq1, Ambreen Kanwal1, Sana Rasheed1, Mabel Baxter Dalrymple2 and Anam Tariq1
... thalassemia major (BTM) patients, the current study was carried on 408 subjects including 204 patients and 204 controls. For all 408 individuals; complete blood count (CBC), blood group, serum ferritin level and liver function tests were performed. Secondary complications were assessed by physical examination of pallor, splenomegaly, ascites, and hepatomegaly. The average±SD values of patient...
Neelam Pery1*, Fatima Ijaz1, Nayab Batool Rizvi1, Munawar Ali Munawar1 and Muhammad Imtiaz Shafiq2*
...ng a large proportion of patients untreated. This is due to complex pathogenesis comprising a network of signaling pathways. By controlling one pathway, the pathogenesis continues through complementary pathways. Janus kinase and bruton’s tyrosine kinase regulates a number of T-cell and B-cell mediated signaling pathways ranging from cytokine expression and antibody production to the bone resorption and cartilage destruction. We propose a dual trap to con...
Xia Li1*, Xueliang Zhan1 and Xinjie Shi2
... failure. A total of 180 patients treated in our hospital due to AECOPD complicated with type II respiratory failure were enrolled in the study and divided into study group and reference group, each with 90 patients. The study group was treated with ulinastatin and ambroxol, while general routine treatment was implemented in the reference group. The treatment outcomes were compared between the two groups. The blood content l...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
...level in serum of cancer patients was observed. For this purpose, the blood of 50 cancer patients was taken from MAYO HOSPITAL Lahore. The patients are suffering from different type of cancers such as, breast cancer, lung cancer, uterus cancer, oral cavity cancer, esophageal cancer, urinary bladder cancer, blood cancer and brain tumor. In addition to serum zinc level of cancer
Yongmei Gao1, Peihong Gao2, Yanjun Wang3, Jinhua Han4 and Yang Shu1*
...as research objects. The patients were randomly divided into an experiential group and a control group, each containing 80 patients. The experiential group was treated with mifepristone and Guizhifuling capsule, and then subjected to high quality nursing. In contrast, the control group was treated with only mifepristone therapy and applied with routine nursing measures. The therapeutic and nursing effects of the two groups w...
Mohsin Ali1, Nargis Haider Kakar2, Rida Dawood3,4, Qurat-ul-ain Fatima5, Zunera Tanveer2, Shoaib Ahmad Malik6, Tauseef Muhammad Asmat1, Asadullah1, Muhammad Masood Tariq Kiani1 and Mohammad Zahid Mustafa1*
... worldwide, and diabetic patients are at risk of having other immune diseases including thyroid abnormalities. The current study, undertaken in collaboration with Shaikh Khalifa-Bin-Zayed Hospital Quetta, investigates the relation of thyroid abnormalities in 224 DM (both type 1 and type 2) patients. The parameters of age, duration of diabetes and type of treatment were measured. Venous blood was taken to investigate the thyr...
Ahmad Sadiq1, Muzafar Shah2*, Habib Ullah1, Irfan Ali1, Amir Alam3,
Navid Jalil1 and Muhammad Khan3
...t-size: small;">Diabetic patients are deliberated to be at greater risk of hypertension and lipid abnormalities, which are the main cause of cardiovascular diseases (CVD). The present work revealed the role of glycated hemoglobin (HbA1c) for the detection of lipid profile and CVD. In recent study 108 (56 males and 52 female) type 2 diabetes (T2DM) subjects were diagnosed at Hayatabad Medical Complex (HMC) Peshawar and private diabetic clinic “Khattak Med...
Jia Shi
...gative chronic hepatitis patients is necessary as they are vulnerable to developing cirrhosis and hepatocellular carcinoma. The present study was aimed to evaluate the response rate of treatment by Peginterferon alfa-2a. In this systematic review and meta-analysis, a review of articles evaluating response rate to Peginterferon alfa-2a was done through the databases of Scopus, Google Scholar, Pubmed (inception - 2019). Fourteen studies reporting the response ra...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...cted from all age groups patients visiting hospital labs and District Head Quarter Laboratory who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found infected with malaria parasite. Comparative Data was also collected from other labs of District Swat which accumulated ...
Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*
...f inheritance as all the patients are homozygous for the mutation while parents were heterozygous and unaffected siblings from both families were either heterozygous for the reported mutations or they lacked this mutation.
...
Ahsan Numan1,4, Abdul Basit Qureshi2, Fehmeda Farrukh Khan3,Faisal Masud3,Ijaz Ahmad4, Kamran Ashraf5, Nisar Ahmad5, Muhammad Shahbaz Yousaf 4, Imtiaz Rabbani4, Hafsa Zaneb6 and Habib Rehman4*
...f sensorimotor nerves in patients with clinical diabetic polyneuropathy (DSP). Clinical diagnosis of DSP was assessed with the Michigan Neuropathy Screening Instrument. The diagnosed patients with DMT2 were randomly distributed into 2 groups. Group-I (n= 79) included the patients with DSP having <10 years of duration of DMT2, called short duration, while DSP pat...
Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6
...IV and HBV-mono infected patients in Jos, Nigeria. This cross-sectional study was conducted on ninety (45 HBV/HBV and 45 HBV-mono) infected patients with chronic hepatitis B attending the hepatitis clinics of the two tertiary hospitals (Plateau State Teaching Hospital and HIV treatment center, at Jos University Teaching Hospital), Jos Metropolis from September to December 2018. The anti-HDV antibodies (IgM) were assayed usin...
Basharat Ahmed1, Muhammad Hamza Azhar2, Farah R. Shakoori1 and Abdul Rauf Shakoori1,3,*
....isolated from diarrheal patients admitted to hospitals in Azad Kashmir Pakistan were analyzed to determine their changing trends in response to fifteen antibiotics. Campylobacter isolates were identified as C. jejuni (66.4%) and C. coli (33.6%). An over whelming majority of isolates were recovered from children (72%), 52 % were from male patients. The highest number of strains was isolated in summer (30...
Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*
...were recorded as general patients in those Upazila Veterinary Hospitals. Surgical affections were arranged based on sex, breed, and seasons. Similar distribution pattern was observed in all three Upazila. Most common surgical affections in calves were atresia ani, 30.82%, 32.64% and 31.75% followed by umbilical hernia 19.45%, 17.78% and 17.62%, navel ill 15.75%, 15.79% and 16.08%, umbilical abscess 14.38%, 15.28% and 14.27%, fracture 9.59%, 8.61% and 10.49%, m...
Mehwish Saleem1*, Maria Tahir1, Maryam Ilyas2 and Aqsa Malik2
...re collected from cancer patients from MAYO hospital LHR during the time period April 2018 to May 2018. Some cancer patients have increased calcium level in blood. Cancer is more prevalent in males (n=17) 57% than females (n=13)43%. The most frequent cancer type among males is blood 41cancer (n=5) 36%, lung cancer (n=3) 22% and oral cancer (n=3)22%. In females breast cancer (n=5) 32% is most prevalent, followed b...
Guangyu Wang, Xu Tian, Lintao Liu, Junyang Liu, Bo Zhang and Jingming Dong*
... and improve function in patients with knee osteoarthritis (KOA) and in traditional Chinese medicine, Shujin Huoxue Tablets (SJHXT) is known to be a useful medicine for KOA. The aim of this study was to investigate the effects of SJHXT combined with sodium hyaluronate on synovial inflammation in a patients with KOA. A total of 180 patients with knee osteoarthritis who were treated in our h...
Maria Qibtia1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Sehrish Faryal1, Ahmed Mansouri2, Zeeshan Ahmad2, Muhammad Hamid3 and Ali Raza Awan1,*
... 2q21. In this study, 50 patients with LI were investigated for the identification of mutations in the LCT-gene. In comparison of 30 subject with lactose persistence (LP) considered as healthy group. A total of 13 genomic mutations were identified, 1 in the promoter region and 12 in the intronic/exonic region of LCT-gene. Among these 12 mutations, 6 of them are novel in origin. The novel mutations were found in intron 4 c.(918+116)A>C, intron ...
Maha I. El Zaafarany1, WafaaM. Badawy2, Eman M. El Salakh3
Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.
...oma (HCC) in a subset of patients. HCC is a prevalent disease in many populations worldwide. It causes many financial problems in treatment modalities with high mortality rates. In develop-ing countries, the major concern in HCC frequently belongs to HCV long lasting in-fection. Chronic HCV infection mostly leads to hepatic cirrhosis before developing HCC. HCV infection induces overproduction of reactive oxygen species (ROS) in hepatocytes leading to their pro...
Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy
...of RV infection and male patients were at higher risk than females. In conclusion RV is a common etiological agent of serious diarrhea in infants and young children. RT-PCR is more sensitive than LFIC and ELISA in detecting RV infection. However, LFIC is a rapid and easy test that can aid in the detection of RV in pediatrics helping healthcare provider in making patient management decisions at the same office visit. Negative LFICA results do not rule out the i...
Roheela Yasmeen1*, Nida Mobeen1 and Samia Chaudhry2
...d its enhanced effect on patients with already existing cardiovascular diseases.
...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...roughout D. I. Khan. The patients included had been married for 3 to 19 years, their wives had normal reproductive capabilities; the couples were living together for more than 3 years but fertilization had not occurred. Separation of sperm samples from semen plasma, sperm counting and DNA extraction for assessment of clinical and molecular attributes was done using standard protocols. Healthy women were used as negative controls. This study revealed significan...
...index in clinical stable patients with chronic obstructive pulmonary (COPD) disease. For this purpose, a retrospective cohort was designed. In this work, 200 patients with COPD at stable stage were enrolled as research subjects. They were divided into the control group (n=100) given conventional treatment (bronchodilator, antioxidant, expectorant antitussive) and the research group (n=100) given pulmonary rehabilitati...
Tian Tian, Lili Luo*, Haiyan Wang and Lei Wang
.... One hundred and eighty patients with ectopic pregnancy were included in the study, all of whom were treated with methotrexate combined with mifepristone tablets. According to the treatment results, they were divided into treatment success group and the treatment failure group, each containing with 90 cases. The levels of β- human chorionic gonadotropin and progesterone in the two groups were compared.Of the 180 patients
Niaz Muhammad1, Samina Yasin1, Zunaira Fatima1, Noor ul Ain1,
Muhammad Faizan2 and Sadaf Naz1*
...orted for achondroplasia patients from other countries.
...
Mona Mohammed EL-Derbawy1, Eman Naser Hafez2*,
Mona Abdel Daym Abd Rabbo1, Nagwa Ibrahim Toaleb3,
Saedia Abdel Hady Sayed El-Ahl1 and Bahaa Eldkeen Wade EL-Aswad4
...ncluded 21 obtained from patients who were positive for schistosomiasis and negative for other parasitic infections; Group ІІ included 18 sera from patients who were negative for schistosomiasis and positive for other parasitic infections; (3) fascioliasis, (5) amoebiasis, (7) toxoplasmosis and (3) hydatidosis and Group ІІІ included (13) sera from healthy individuals all were diagnosed parasitologically and serologicall...
Muhammad Mansha1*, Sidra Yaqub1, Asma Abdul Latif2, Muhammad Wasim3 and Peter C. Thomson4
...wo hundred breast cancer patients from INMOL hospital, Lahore (Pakistan) were interviewed on reproductive factors. HER2/neu levels were determined by ELISA from 52 patients and 37 age-matched control women. The results of survey study showed that “premature termination of pregnancy” emerges as a potential risk factor of breast cancer.
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...d inheritance pattern in patients of Karak region, Khyber Pakhunkhwa (KP) province, Pakistan. During the study, 200 peripheral blood samples were collected both from families having at least one transfusion dependent child and sporadic patients from different areas of karak region. Using the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique, all samples were analyzed for the most common ...
Longjun Guo*, Jiaqi Teng, Juan Wang and Yukun Chen
...ression in leukoaraiosis patients. Inpatients who attended the Department of Neurology of our hospital from January 2018 to December 2018 were selected as study subjects, with 58 patients with leukoarais as the experimental group, and 60 patients without leukoarais in the same period as the control group. ELISA was used to detect τ (Tau) protein expr...
Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1
...icipants comprised of TB patients (100), livestock(L/S) farm employees(200), abattoir employees(174), butchers(294), veterinarian(10) and veterinary assistants(22). Two out of 100 TB patients (2/100), 3 out of 200 livestock farm workers (3/200) and 3 out of 23 abattoir workers (3/23) were found positive for the presence of M bovis through PCR technique. A significant association (p<0.05) was found between livestock farm w...
Tabinda Urooj1,*, Bushra Wasim1, Shamim Mushtaq2, Syed Nudrat Nawaid Shah1, Lubna Faisal3, Moazzam Ali2, Nabeela Rasheed1 and Syed Faizan Ali Rizvi4
...out of which 121 (69.2%) patients were untreated (Group A). The remaining 54 (30.8%) patients, who received cancer treatment as adjuvant therapy, were included as treated (Group B) cases. Regarding patient’s demographic data, mean age of enrolled patients was found to be 48.26±10.74 years. The mean age of menarche was found to be 13.39±0.89 years. The calculated BMI (bo...
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
...erotypes to hospitalized patients in Assiut Governorate, Egypt, as well as, to assess their pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Salmonella with the acquisition of shish-tawook (14%). On the other hand, 100 hand swabs that were assembled from food handlers working in the sam...
Yan Jiang1,2, Xia Tao3,4 and Hongxia Chen5,6*
...igned for this study. 92 patients with IMN in our hospital from December 2015 to December 2017 were divided into study group (n=46) and control group (n=46) based on a computer-generated random number table. On the basis of conventional treatment, the control group was given prednisone and cyclophosphamide treatment, and the study group was given rituximab-assisted prednisone and cyclophosphamide treatment, both of which were treated for 6 months. The efficacy...
Zheng Tan*, Li Li, Yuxin Song, Meirong Tian and Fan Jia
...ify;">In this study, 150 patients diagnosed with coronary heart disease (CHD) were divided them into three groups, each containing 50 patients, namely stable angina pectoris (SAP) group, unstable angina pectoris (UAP) group, and acute myocardial infarction (AMI) group. Fifth healthy people were enrolled as control. The changes in serum endothelin, brain natriuretic peptide and high sensitive c-reactive protein in
Muhammad Waqas1, Naveed Ahmed2*, Hamid Saeed Malik1, Unaiza Qamar3, Hasnain Javed4 and Ahsan Hussain1
...eta;-thalassemia carrier patients with frequency of different common and uncommon/uncharacterized mutations that are IVS 1-5 (G-C), Fr8-9 (+G), Fr 41-42 and Cd 5 mutations of β-thalassemia traits. A total 293 β-thalassemia carrier patients were included in study. Fr 8-9 (+G) was found the most common mutation of β-thalassemia carrier patients in the population which was 112 ...
Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2
...nd unacceptable for most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the diagnosis of infection with H. Pylori in human stool and fasces of dogs and cats. Two hundred stool samples from humans and eighty-eig...
Yin-Hua Wang1,2, Yi-Bing Zhu1, Li Jiang1, Bin Du3 and Xiu-Ming Xi1*
...e pain in critically ill patients, especially for those under mechanical ventilation (MV). Remifentanil, a potent μ-opioid receptor with a rapid onset and offset, is widely used in anesthesia during surgeries, whereas less commonly administrated in the intensive care unit (ICU). This study is designed as a prospective, randomized, double-blind, controlled, parallel-group trial. Eligible 254 adult patients in ICU requiring...
Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3
...ical diagnosed asthmatic patients (Diseased) and 19 non-asthmatic patients (Control). The female was 6 (31.6%) and males were 13 (68.4%) in control group while females were 9 (52.9%) and male were 8 (47.1%) in asthmatic group. Mean age (49.59±15.82 year) and BMI (21.96±06.67 kg/m2) were recorded for asthmatic group while mean age and BMI for control group were 32.82±12.50 year and 18.87±05.17kg/m2...
Fujun Miao1, Chunlan Shan2, Wei Yang3, Hao Wang3, Shuxiang Geng1 and Delu Ning1*
...tary supplement for some patients. In this study, the protective effects of walnut oil isolated from Juglans sigllata on livers of alcoholic liver disease (ALD) mice were studied. The results showed that serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the ethanol + walnut oil (ETH + WO) and ETH + silymarin positive group (PC) groups significantly decreased, and the lesions of ethanol-induced liver injury were relieved compared with...
Hong Liu and Rui Xu*
...tion of fetal kidneys in patients with pregnancy-induced hypertension. It is concluded that 3D-CPA ultrasonography could be used to quantitatively analyze fetal kidneys with gestational hypertension in pregnancy, providing a quantitative basis for prenatal diagnosis of fetal renal blood perfusion in patients with gestational hypertension. It could also be used to detect risk pregnancy in early pregnancy....
Nan Jiang, Tong Liu, Jimin Liu and Guobin Tang*
...tion. In this study, 180 patients who had been diagnosed and treated for bronchial asthma in our hospital were enrolled as research objects. They were divided into experimental group accepting inhalation of low-dose budesonide powder combined with pulmonary rehabilitation and control group accepting conventional drug therapy. The therapeutic effects of the two groups were compared. Compared with the control group (77.78%), the overall treatment effective rate ...
Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz
...ne. Blood samples of 116 patients having tested for multiple drug resistance were analyzed for blaNDM-1 gene by PCR. Sixteen samples were found to be positive for blaNDM-1 gene. The bacterial species harboring blaNDM-1 gene were 25% Enterobacter cloacae, 18.75% Klebsiella sp., 12.5% Pseudomonas sp., 12.5% Citrobacter freundii, 12.5% Acinetobacter Baumanii, 12.5% E. coli and 6.25% shigella sp. Nucleotide sequencing of PCR product of Klebsella sp, Enterobacter c...
Ying Feng1 and Xuqun Pei2,*
...tophagy and apoptosis in patients with ischemic stroke and cognitive impairment. Glucose metabolism abnormalities and cognitive function in patients with ischemic stroke were detected by glucose tolerance test and neuropsychological test. Morris water maze test was used to detect learning and memory ability of MCAO model rats. RT-PCR and Western Blot method were used to detect the expression of autophagy and apoptosis genes ...
Ejaz Ali and Nageen Hussain*
...ions in hearing-impaired patients of Gilgit-Baltistan is 11.66 %. The mutation rate is higher as compared to other populations of Pakistan. This study can help deaf patients via gene therapy and also through drug designing....
Zhan-Zhong Liu1, Chun-Ying Wang1, Jian-Ye Yang2, Qing-Hua Liu3,4, Xiao Zhang3* and Liang Wang3,4*
...ocusing on all confirmed patients with COVID-19 in Xuzhou, a prefecture-level city, and a transportation hub with 10.44 million population in the east region of China. The median age of the patients is 42-year-old and 45.57% are male; 25 cases (31.65%) are imported. 23 cases (29.11%) were confirmed between January 26 to 31, 2020 while 56 cases (70.89%) were from February 1 to 16, 2020. Among the ten administrative divisions ...
Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5
...c sequence variants. CEP patients were identified using successive clinical tests. Blood samples of patients were collected and processed for genomic DNA extraction followed by Sanger sequencing to identify pathogenic mutations in UROS gene. Sequence analysis revealed a pathogenic missense mutation (c.935T>C [p. L237P]) in the exon 10.The sequence was further analysed in-silico to determine the effect of pathogenic mutati...
Muhammad Altaf1* Arshad Mahmood Abbasi2, Muhammad Shoaib Amjad3, Sadia Naseer1 and Muhammad Umair4
...cumented in all COVID-19 patients tested provides confirmation of a link between the presence of this virus and the disease....
Jun Zhang1*, Xiaocao Xu2 and Min Cao2
...acted and collected from patients’ records, including gender, age, number of blood transfusions per year, HBs-Ag, HCV-Ab and Anti-HBs. The study included 630 thalassemia major patients who received regular blood transfusions at a hospital in Bengbu, China. We found that out of 630 patients of the study, there were three HBs-Ag positive, so the prevalence of HBV in this study is 0.5%....
Qi Zhao1, Yuanyuan Hou1, Xiangdong Jin1 and Chen Zhang 2,*
...litis. One hundred sixty patients with ulcerative colitis were divided into the experimental group accepting mesalazine combined with BTV and the control group accepting mesalazine alone. It was found that the overall treatment effective rate was 93.75%, in the experimental group as against 77.50% in the control group, p<0.05. Comparing the levels of inflammatory factors (IL-6,IL-8,TNF-α) before and after treatment in the two groups, the improvement d...
Maria Qibtia1, Sehrish Faryal1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Ahmed Mansovuri2, Zeeshan Ahmed2, Muhammad Hamid3 and Ali Raza Awan1,*
...Ps with LNP in Pakistani patients. Our analysis has evaluated the association between the development of LNP trait and two common variants in intron 13 and 9 (13910-C/T and 22018-G/A) along with the P-value significance level. Therefore in this study, we aimed to access genetic predisposition and clinical manifestations of LNP and LP in 80 subjects (30 subjects with LP and 50 LNP). The presence of T-13910, C-13913, G-13915, G-13907, C-3712, C-13779, A-13937, G...
Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*
... included 66 hepatitis C patients (genotype 3) who responded to the directly acting antiviral treatment and achieved negative HCV-RNA three months after completing the treatment (sustained virologic response (SVR)) and 66 hepatitis C patients (genotype 3) who did not achieve SVR three months after completing the same treatment. Informed consent was taken from participants, demographic data was collected, and 5 mL of blood wa...
Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*
...cohort of cardiovascular patients. While their structural impact on APOB may suggest these predicted nsSNPs possibly be a better drug target and contribute to the treatment and better understanding of human cardiovascular disease....
Nawal Abd-elhafez Hassanain1, Raafat Mohamed Shaapan1*, Ahmed Maher1, Ahmed Badwi Yousif2, Gomaa Desoky Emam2
...he study aimed to screen patients attending El Fayoum and El Zigzag (El Sharkaria governorate) fever hospitals presenting with malaria-like symptoms for the possibility of false-positive malaria diagnosis by card test RDT (Rapid Diagnostic Test) due to typhoid fever and brucellosis and finally to determine if the associated individual characteristics were serologically positive to these diseases. The collected 504 blood samples were screened for malaria (card ...
Ahmad Ali1, Zaib Ullah2, Muhammad Javaid Asad1*, Muhammad Rafi3, Muhammad Maqsood4 and Sajid Mahmood5,6
...rget sites of the cancer patients. It also the importance in medicines preparation of the specific target cells.
... 
Muhammad Kashif Maan1*, Rohma Shabbir2
...hydatidsis in veterinary patients. A 2-year ram was presented to Surgery teaching hospital of University of Veterinary and Animal Sciences, Lahore with the complaint of progressive exophthalmos with proptosis without pain, disturbance in ocular motility, visual deterioration, and chemosis. There was no evidence of presence of cyst in lungs and liver upon radiographic examination. Rhinotomy approach was used to enucleate the Cyst. There was no postoperative com...
Zhaojun Wang1, Yang Zhou2 and Xinghua Song3*
... 120 knee osteoarthritis patients admitted to our hospital from January 2019 to February 2020 were selected as the study subjects and randomly divided into control group and observation group (60 cases each). The control group was given injection of sodium hyaluronate in knuckle articular cavity, and the observation group was further treated with compound betamethasone. Clinical efficacy of the two groups was observed after treatment, the knee joint pain befor...
Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6
...ipants including 261 CVD patients and 243 healthy individuals were enrolled after taking informed consent. The analysis of the three allelic variants GSTM1, GSTT1, and GSTP1 was carried out through PCR-based amplification. Amplification of GSTM1 and GSTT1 was performed using the specific primers designed by Primer-3 software. GSTT1 and GSTMI genotypes were determined by comparing the sizes of amplified PCR product of genotypes with β Globulin gene, used a...
Jiacheng Du and Yuping Cao*
...and pregnancy outcome in patients with the polycystic ovarian syndrome (PCOS) who underwent Invitro fertilization-embryo transfer (IVF-ET). This study selected 200 patients with PCOS who took IVF-ET in our hospital from January 2016 to January 2020 as subjects. The study subjects were divided into group A (low-level group, n=90) and group B (high-level group, n=110) according to serum AMH levels; and group C (successful grou...
Xiaoli Yao1, Xiaofang Yue2, Yue Yang3 and Guobin Zhang3,*
...nfarction. A total of 80 patients with acute cerebral infarction were randomly divided into 2 groups each of 40 cases, regardless of gender, aged from 55-75 years, who underwent treatment in hospital from January 2018 to December 2020. Each group received routine treatment, in which the treatment group received LMWHC alongwith argatroban. The score of neurological deficit (NIHSS) and serum neurological function, endothelial injury and inflammatory marker level...
Xiaoli Yao1, Xiaofang Yue2, Yue Yang3 and Guobin Zhang3,*
...nfarction. A total of 80 patients with acute cerebral infarction were randomly divided into 2 groups each of 40 cases, regardless of gender, aged from 55-75 years, who underwent treatment in hospital from January 2018 to December 2020. Each group received routine treatment, in which the treatment group received LMWHC alongwith argatroban. The score of neurological deficit (NIHSS) and serum neurological function, endothelial injury and inflammatory marker level...
Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5
...enes polymorphisms in TB patients of Pakistani population. A total of 260 study participants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with EPTB (n = 40). Tri and Tetra- ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant association (OR, 2.1 (0.9...
Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1
...spital acquired infected patients. The recovered isolates were identified biochemically by vitek2 compact, confirmed by PCR and tested for sensitivity against varieties of antimicrobials. Three multi-drug resistant isolates carried ica from human abscess (A9), sputum of dog (A12), and cow mastitic milk (A13) were sequenced with other genome sequences retrieved from the GenBank. Ten samples (9.17%) were S. aureus from human, while 3 samples (1.24%) were S. aure...
Fengbin Jin1, Xin Liu2 and Yanqiu Yue3*
...e asthma. A total of 114 patients with severe asthma of hospital were selected between December 2017 and February 2020 as the subjects of this study and divided into the observation group and the control group randomly. Patients in the control group received the regular treatment, while those in the observation group received the treatment by low-dose epinephrine, and the efficacy of these two methods was compared between tw...
Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3
... therefore, treatment of patients with dual infection is indeed a challenge for clinicians. A timely and accurate diagnosis is critical for treatment in this regard. Expression and purification of HDAg, which is the only protein of HDV of the local isolate is the primary objective of the study. This antigenic recombinant HDAg protein can be useful for both vaccine development and as a diagnostic marker of HDV. After determination of HDAg antigenic region of HD...
Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1
...aracterize breast cancer patients of district Bannu, KP, Pakistan for any possible polymorphism in the estrogen receptor (ESR1) gene. Blood samples in this regard were collected from clinically diagnosed eighteen breast cancer patients, all possessing invasive ductal carcinoma. DNA was amplified through PCR for 2 different sequences of ESR1 gene. Twelve normal individuals of different ages were also characterized. Amplified ...
Lei Xu1, Qin Yang2 and XiaoYan Zhang3,*
...of sclerosin in serum of patients after peritoneal dialysis (PD) and to analyze the underlying clinical factors, a total of 94 patients who were subjected to the peritoneal dialysis for over three months in the Nephrology Department of XXX Hospital between January 2019 and January 2020 were enrolled into the experiment group (n = 94). Simultaneously, 94 healthy subjects who received the physical examination were enrolled int...
Huajun Lin, Wei Qu and Zhuxian Yu*
...side in the treatment of patients with acute carbon monoxide poisoning (ACMP) and its effect on patients’ cognitive function. A total of 64 patients with ACMP treated in our hospital from January 2019 to March 2020 were selected as the research object, and these 64 patients were divided into an experimental group and a control group by random numbe...
Jiangbo Shao1, Donglai Zhu2, Shengqiang Zou1, Guohong Ge1 and Ju Huang1*
...hepatocellular carcinoma patients. These results suggest that SOX9 participated in hepatocellular carcinoma progression through targeted regulation of HSPA1B that might provide novel insights in hepatocellular carcinoma therapy....
Saydat Saad Abd El-Megeed1, Walaa Yehia El-Sayed1*, Tarek Khamis2
...se of the vast number of patients affected by the condition and the repercussions it has, many scientists are being forced to research multiple medicines, all of which are quite expensive. The antidiabetic activities of zinc oxide nanoparticles (ZnO NPs) were assessed in rats. ZnO NPs were characterized by transmission electron microscopy (TEM). Diabetes was induced by streptozotocin (STZ) -nicotinamide in rats, and some biochemical and molecular parameters we...
Man Wang1 and Fengmei Yang2*
... survival probability of patients with high expression of TOP2A gene were lower than those with low expression of TOP2A gene (P<0.05). In clinical specimens, the expression of TOP2A in cancer tissues were significantly higher than that in the normal tissues, and were related to Edmondson grade. To conclude, the methylation of TOP2A gene and the infiltration of tumor-associated immune cells may play an important role in the pathogenesis of liver cancer, and ...
Humera Manzoor1,2,5 Norbert Brüggemann2,3, Hafiz Muhammad Jafar Hussain1, Tobias Bäumer2, Frauke Hinrichs2, Muhammad Wajid4, Alexander Münchau2, Katja Lohmann2* and Sadaf Naz1*
...omozygosity in the three patients and heterozygosity in the obligate carrier (mother), predicted effect of variants on the encoded protein, and their frequencies in public databases. Sanger sequencing was performed to explore the segregation of the variant with the phenotype. All patients had congenital limb contractures. These included camptodactyly of hands and feet, ptosis, adducted thumb and clubfoot morphology. A novel ...
Mingqing Wang1 and Xiaojuan Chen2,*
...the treatment failure in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and many studies have tried to specify an effective treatment. Objective of this study was to observe and analyze the therapeutic effect of glucocorticoid combined with respiratory stimulant on patients with AECOPD along with the optimal nursing care required for these patients. Also...
Asma Basharat1, Abdul Wajid2*, Andleeb Batool1, Tayyeba Batool3, Abdul Basit4, Kamran Abbas5, Aziz Ullah1 and Mahmood Shaukat6
...1 CP clinically assessed patients and 83 healthy controls. Our preliminary results revealed two novel missense substitutions i.e. P180Q in T-box domain and S328I in C-terminal of TBX22, while unrelated healthy controls revealed no sequence variants in these locations. The present report is first of its kind in Pakistani population. The findings suggest that TBX22 polymorphisms may responsible for a significant proportion of non-syndromic CP cases in Pakistani ...
Jianmin Liu1, Jiancheng Zhang2, Peijuan Wang3,* and Moslem Lari Najafi4
...function decline. Ninety patients with ovarian function decline were randomly divided into 3 groups: new addition congrong tusi pill treatment group (n=30 cases), diethylstilbestrol treatment group (n=30 cases) and the control group (n=30 cases). A comparative analysis is made on sex hormone levels, ovarian artery blood flow, ovarian volume and number, treatment efficacy, glucose metabolism, ovulation and fertility in each group. There are statistical differen...
Farah Naz1*, Asad Umair2 and Ghazala Noureen3
...rwhelmed by too many new patients. However, lockdowns and related strategies designed to minimize the spread of the pandemic, like early detection, isolation of confirmed cases, and social distancing, were build and implemented on the assumption that COVID-19 is simply a health emergency that demands solutions from the life science alone. They failed to take into account structural inequalities present in societies that affect the ability of an individual to c...
Eighty Mardiyan Kurniawati1*, Budi Santoso1, Fedik Abdul Rantam2, Widjiati3, Budi Iman Santoso4, Tri Hastono Setyo Hadi1, Gatut Hardianto1, Hari Paraton1
...wound healing process in patients but still needs to be studied in humans.
Keywords | Freeze-dried amnion, Wound healing, Vesicovaginal fistula, Histology, New Zealand rabbit model, Angiogenesis and obstetric disturbances
...
Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*
...eneficial impact for the patients associated with arthritis....
Sheeza Iqbal* and Asima Azam
...rs among male and female patients. The cross-sectional study was conducted at Qazi Hussain Ahmed Medical Complex from March to July 2020 in district Nowshera, Pakistan. A total of 150 patients with sciatica were analysed. All the patients were diagnosed with MRI, CT scan, spinal X-rays and straight leg raising (SLR) test. Of the 150 sciatica patients, 88...
Cuiping Liu
...urification treatment in patients with maintenance hemodialysis (MHD). 106 patients with MHD were treated in our hospital from February 2017 to January 2020. They were divided into observation group (n=53) and control group (n=53) using a random number table. The control group was given heterozygous blood purification, and the observation group was given alpha keto acid combined with heterozygous blood purification for 6 mon...
Seema Perveen Memon*, Nasreen Memon, Mansoor Ali Shah, Reshma Sahito and Aiman Amur
...Monthly data of malarial patients were collected from Anti-malaria control program (AMCP) of different localities of district Sanghar. Total 26161 suspected cases of malaria were examined, 490 patients were found malaria positive. Among these positive cases, 477 patients were found Plasmodium vivax and 13 cases with Plasmodium falciparum. Gender wise and age wise malaria were examined, max...
Seyedmohammad Karimi1, Mohammad Darvishi2*, Mohammad Barati3, Ramin Hamidi Frahani4, Mohammad Hassan Kazemi5
...clinical trial study, 70 patients with Covid-19 in Besat Hospital, Tehran, Iran, during 2022, were enrolled. Patients were randomly divided into two groups of 35 each. Both groups were administered Remdesivir with same protocol. In this period, the first group received N-acetylcysteine 600 mg tablet twice daily and melatonin 6 mg tablet at bedtime. The second group received placebos with the same appearance. Liver enzymes of...
Muhammad Shahid1*, Muhammad Idrees1, Afza Rasul2, Iram Amin1 and Samia Afzal1
...nguish the potential HCV patients from healthy individuals at an early stage is quite important for its control, which can be achieved by investigation of liver enzymes, complete blood count, and lipid profile of patients. In this study, 144 CHC and 20 controls were included. The serum of CHC patients was analyzed for HCV viral load and genotype. The liver function test, lipid profile, sug...
Bin Hu1*, Jianchang Qu2, Tenglong Han3 and Jun Leng1
...d blood sugar control in patients with diabetes and periodontitis. A total of 72 diabetic patients with periodontitis who were treated in our hospital were taken as the expirmental objects, as the control group (n=36) and the study group (n=36). Patients in the control group received normal diabetes treatment and basic periodontal treatment, and the study group received probiotic treatment...
Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1
ASIMA BANO1, HAFIZ MUHAMMAD TAHIR*2, AROOSA RASHEED3, MUHAMMAD ARSHAD4, SHAFAAT YAR KHAN1 & RABIA YAQOOB5
...terases in breast cancer patients. For study blood samples were collected from various diagnostic laboratories of Sargodha city. Levels of non-specific esterases were determined in the normal and breast cancer patients. The levels of non-specific esterases were higher in healthy individuals than the breast cancer patients. So, levels of non-specific esterases could be used as a marker for ...
MUHAMMAD MUNEEB SOHAIL1, FATIMA AMEER2, GUR CHARN SINGH2, MUHAMMAD ZAID1*
... 21% of non-dyslipidemic patients. Males were more inclined towards lipid abnormalities than females especially HDL abnormality. Abnormal HDL levels were mostly associated with the rural population. But consumption of fast food may prove fatal for lowering the HDL level in the urban population also. This data gathered may have implications in the characterization and diagnosis of diseases but it can prove helpful in assessing their severity and type.
SAIMA SHARIF*1, SHAGUFTA NAZ1, RIDA IRAM1, FARKHANDA MANZOOR1, TASNIM FARASAT1 and MUHAMMAD SAQIB2
...eatly altered in anaemic patients.
Key Words: Anaemia, Children, Risk factors, rural areas
...
Caiping Duan
...njury in type 2 diabetic patients. In the present study, 369 patients suffering from myocardial ischemia-reperfusion injury who were diagnosed with type 2 diabetes in our hospital from January 2018 to May 2019 were selected as study subjects and randomly divided into low-dose propofol group (n=123; intravenously injected with 25 mg before angiography), high-dose propofol group (n=123; intravenously injected with 50 mg before...
Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3
...ing to the fact that HCV patients can develop HCC, plant extract has also been tested for cytotoxic activity. Colorimetric analysis was done to determine the vitality of HepG 2 cells for 24 h after treatment with methanolic seed extract and minimum inhibitory concentration was calculated. HepG2 cell were transfected stably to express nonstructural proteins NS3 and NS5A of HCV 3a genotype. Western blot and RT PCR confirmed stable protein expression. Immunofluor...
Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal and Gehn Allam
... positivity was found in patients with age less than 25 years, and in patients with young age at marriage (≈ 20 years). High parity and use of hormonal contraceptives were found to be associated with high frequency of HPV positivity in aborted patients. On conclusion. human Papillomavirus types 16 and 18 might have a role in the occurrence of the first trimester spontaneous abortion ...
Pengliang Xie1, Lufang Zheng2*, Mingxia Dong3, Huaiqiang Zhang1 and Fang Chen1
...lar edema. A total of 45 patients with branch retinal vein occlusion and macular edema who were treated at Tangshan Eye Hospital from November 2018 to June 2020 were enrolled in the study, including 19 cases of localized macular edema, 12 cases of diffuse macular edema, and 14 cases of cystoid macular edema. Forty-five healthy people in physical examination were recruited as control. We found that the diseased group had higher proportion of hypertension (P<...
Wuritunashun*, Burenbatu, Narenqiqige, Jiuguniang, Eerdunduleng, Shuanglian Wang, Cuiqin Gong, Hashengaowa, Huizhi Jin, Baiwurihan and Chunhaizi
...rotein expression of HSP patients. Out of 14 significantly differentially expressed proteins, four proteins immunoglobulin lambda variable 3-27, immunoglobulin kappa variable 3-11, neural cell adhesion molecule L1-like protein, and immunoglobulin heavy variable 3-33 were upregulated, whereas 10 proteins i.e., protein Z-dependent protease inhibitor, C4b-binding protein alpha chain, heparin cofactor 2, complement C4-A, complement C4 beta chain, vitamin K-depende...
Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1
... the major problems that patients face through the treatment, which makes them prone to bleeding. PRP injection therapy is a therapeutic method in use to relieve this condition. It may lead to infections due to the injection, as well as platelet resistance. Therefore, an attempt was made to find an alternative treatment, and the recombinant Interleukin 11 (IL-11). In this study, IL-11 gene owning two restriction enzyme sites of BamHI and XbaI with a final size...
Ahmad Shahzaib1*, Tabish Raza2 and Aisha Areej2
...n, while the rest of the patients are asymptomatic or have minor symptoms. In addition, many COVID-19 patients have co-infection or secondary infection, which exacerbate the disease. In general, it is believed that viral infections predispose patients to superinfections that have much worse consequences than the infection alone. Notably, the latest reports of high mucormycosis mortality an...
Martin-Luther Oseni Okolo1, Monday Eneojo Akor1, Cornelius Arome Omatola1*, Zainab Penninah Ochada1, Ugbane Eleojo Okolo2, Benjamin Mudi Idache3, Andrew Isiramen4, Julius Akor Omatola5 and Moses Adaji David6
...pert in HIV seropositive patients attending Kogi State Specialist Hospital, Lokoja. The study was done using 325 confirmed HIV patients (86 males and 239 females). A structured questionnaire was administered and 325 patients who consented were enrolled in the study. Sputum specimen was collected from each patient, processed, and examined using Xpert® MTB/RIF Assay Version 4. The preval...
Lipigwe Lauya1*, Peace Nkiruka Okeke2, Chukwudi Chizorom Ibeh1, Bello Ozovehe Banimoh1 and Nanma Tongnan Cosmas1
...ss to follow-up of HIV-2 patients, the pathogenicity of the HIV-2 virus, and the clinical picture of the disease depending on the sex of individuals....
Umara Amir ud Din1, Waqas Ahmed Khan1*, Khurrum Shahzad1,2, Basharat Ali3, Misbah Hussain1,4 and Fazli Rabbi Awan4
...nd mortality in diabetic patients. A major cause of DKD is high blood pressure (hypertension) which remains uncontrolled chronically. Renin angiotensin aldosterone system (RAAS) is a major regulator of the blood pressure, electrolytes and fluid homeostasis. RAAS plays a key role in modulating the sodium metabolism, vasoconstriction, vascular tone and renal hemodynamics. Angiotensin converting enzyme (ACE) is a key member of RAAS pathway and target for anti-hyp...
Xiaoyan Yang1* and Xiuli Xu2
...tudy was performed on 50 patients admitted to the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine during the autumn and winter seasons with myocardial infarction and 50 patients as controls. History, tests, and clinical examinations in both groups did not show diabetes, hypertension, dyslipidemia, or smoking. For both groups, Influenza-like experiments were performed in the form of fever with co...
Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*
...has become breast cancer patients. Genetic polymorphisms of the Interleukin-10 (IL-10) gene were studied at position -1082 (G/A) in breast cancer patients in the Pakistani population. Breast cancer patients (n= 200) were recruited from different local tertiary care hospitals along with healthy subjects (n=100) for molecular analysis of the human IL-10 gene mutation at the -1082 (G/A) posit...
Aneela Ahmed Tagar1*, Mahvish J. Channa2, Kanwal Ahmed Tagar3 and Kaka Kainat4
...ion and mental health of patients. The study was conducted in the civil hospital Hyderabad and LUMHS Jamshoro. A total number of 65 post-COVID-19 patients were selected for the study with negative RT PCR tests. A questionnaire was filled after taking consent of patients whereas blood samples were collected to detect the biomarkers (Ferritin, D-dimer, C- reactive protein, LDH, CBC and tropo...
Farooq Jan1*, Abdur Rauf1, Ikramullah Khan1, Muhammad Yasin2, Muhammad Qayash3, Hazrat Wali1, Muhammad Luqman1, Fayaz Asad4 and Muhammad Khalid5
...h are useful for allergy patients. The results showed that mean temperature, wind speed and rainfall are the factors that influence pollen of Broussonetia papyrifera only during the spring seasons and the Dandelion, Cannabis sativa and Eucalyptus globulus pollen grains are influence throughout the year. As a result, the total pollen counts in the atmosphere increased due to Broussonetia papyrifera throughout the year....
Kazi Afsana Homayra Orchy1, Mst. Antora Akter1, Nelema Yesmin1, Md. Moshiur Rahman Khan1, Marzia Rahman2, Md. Mahmudul Alam1*
...ificant increase in burn patients of the saline-treated control animals, whereas, the animals treated with hPRP exhibited the normal range of these functional biomarkers. On the basis of morphology, histopathology, and biochemical analysis, it can be concluded that PRP, irrelevant of source improves and accelerates the burn wound healing with no adverse effects ...
Amna Mushtaq, Asia Bibi* and Nahid Kausar
...logical aspects in these patients, we conducted current study to assess psychotic distress and infertility in PCOS patients of southern Punjab, Pakistan. In this study 204 PCOS women and 150 controls were enrolled. Data of recruited patients was collected from different hospitals of South Punjab. A standardized questionnaire was used for obtaining formal consent and ethical approval. Our f...
Muhammad Ashfaq ur Rahman1*, Saleem Khan1, Aurang Zeb2, Zia ud Din1 and Zafar Iqbal3
...um conversion (SC) in TB patients in Pakistan. A cross-sectional study was conducted at the Baghdada TB Center, Mardan, Khyber Pakhtunkhwa province of Pakistan. The study comprised 100 newly diagnosed TB patients who had tested positive for acid-fast bacilli (AFB). Participants underwent TB therapy in accordance with the WHO recommendations. The variables measured included weight, height, BMI, body fat, albumin and sputum te...
Hafiz Muhammad Arsalan1, Amina Arif1 and Muhammad Khalil Ahmad Khan2*
...hemical profiling of CML patients in response to imatinib or nilotinib therapeutic drugs. Fresh venous blood sample (10 mL) of 170 CML diagnosed patients and 10 healthy individuals was collected in heparin vial from oncology department, Mayo hospital and Jinnah hospital Lahore, Pakistan. Biochemical profiling of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), nitric oxide (NO), advanced ...
Kiran Afshan1*, Sobia Baseer1, Shanza Kiran1, Ghulam Narjis2 and Sabika Firasat1
...matic in immunocompetent patients but the acquisition of the infection during pregnancy can lead to abortions and other congenital defects. The present study aimed to find the seroprevalence of T. gondii among suspected women who visited local hospitals in Khyber Pakhtunkhwa (KP), Pakistan. Sera of 425 suspected women were screened by latex agglutination test (LAT) and enzyme-linked immunosorbent assay (ELISA) was performed to measure the IgG and IgM antibodie...
Frequency of Non-Motor Clinical Features of Parkinson Disease and the Associated Factors in Pakistan
Jaiperkash Moolchandani1*, Geeta Moolchandani2, Syed Mohsin Turab3, Nasima Iqbal4, Ambreen Rehman5 and Shaima Sultana Memon6
...ely. About two thirds of patients were of age 50-60 years with male predominance. It was noted that depression was more prevalent with frequency of 54.3%, anxiety was 41.4% and insomnia was present in 32.9% patients of Parkinsonism. The non-motor symptoms were common among patients who were in their 6th decade of life. Likewise, male patients were much m...
Wei Wang1, Meifeng Zhou2, Yuebo Liang1, Fan Zhang1, Zhong Wu3, Shaowei Mo4* and Yi Qing Wang1*
...ed from 40 breast cancer patients for clinical studies. For in vitro studies, human breast cancer cell lines SK-BR-3-TS was obtained, and trastuzumab resistant model SK-BR-3-TR was constructed. Cell viability was determined using MTT assay. Cell apoptosis was analyzed by flow cytometry. Protein and mRNA expression was measured using western blotting and RT-qPCR, respectively. The mRNA and protein level of YAP was significantly increased in the tumor tissues of...
Abdullah Alsrhani1*, Aisha Farhana1, Shahid Hussain2 and Muhammad Ikram Ullah1
...nd ferritin levels in HT patients....
Jialing Zhang1, Yan Ma2, Lili Lian3 and Min Wei1*
... This study included 180 patients with severe endometriosis who were treated in our hospital. They were divided into the study group receiving GnRH-a treatment alone and the reference group receiving GnRH-a and gestrinone treatment. Patients in the reference group received routine nursing, while patients in the study group received comprehensive nursing intervention to compare the treatmen...
Asifa Majeed*, Amir Rashid, Palvasha Waheed, Asma Faryal, Aden Razaq and Ayesha Maryam
...demia in type 2 diabetic patients. A total of ninety subjects of both genders, aged between 30-70 years were randomly selected and further divided into a diabetic dyslipidemia group, a diabetic group and a healthy group. Genomic DNA was extracted from peripheral blood and subjected to the polymerase chain reaction using primers of the APOA1 gene and ABCG1 gene. The amplicons were subjected to Sanger sequencing on Automated DNA Genetic Analyzer. The seque...
Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and Muhammad Idrees1
...ctive and inactive state patients from four different a Pakistani provinces. Blood samples along with patient history and demographic data were collected from 100 (n=100) patients, all of these were positive for HBsAg (further characterized as acute or chronic hepatitis B). Viral titer was quantified from the HBsAg positive samples by real time-PCR, HBeAg by ELISA, and HDV RNA was detected by nested PCR and sequencing. HBV D...
Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*
...iological events in burn patients. Excessive productions of free radicals are harmful and implicated in tissue damage and multiple organ failure. Supplementation of antioxidants has proven beneficial in decreasing free radicals in burns. The aim of the present study was to investigate the effect of N-acetyl cysteine (NAC) on biochemical alterations during the post-burn stage in scalded rats. Twenty-four male Sprague-Dawley rats were anesthetized and dorsum was...
Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2*
...llected from adult women patients suffering from UTIs during a period from February 2022 to July 2022 in Diwaniyah city Iraq . All urine samples were incubated aerobically at 37°C for 24-48 hrs on blood agar, MacConkey agar as well as biochemical tests identified special media and the isolates. The isolates were confirmed and diagnosed by Vitek assay. In this study, forty-eighth adult female rabbit aged (6-8 weeks) and weighing between (1500 and 2000) gm....
Haiyan Nan, Ran Feng, Guiling Zhang and Jingqin Liu*
...rotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM). In this study recorded patients who were admitted to the No.1 hospital of Baoding from August 2017 to January 2020. We continuously observed the comprehensive diabetes complications screening or blood glucose of 517 hospitalized patients with T2DM. 67 subjects were excluded due to incomplete physical examination and cl...
Akram Ali Baloch1*, Adeel Ahmad2, Kaleem U. Kakar3, Sara Naudhani1, Samiullah Khan1, Agha Muhammad Raza3, Imrana Niaz Sultan1, Humaira Zahid4, Saadullah3 and Shakeela Daud1*
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*
...options for osteoporotic patients, along with their long-term use safety concerns, our research group focused on investigating efficacious, inexpensive and safe alternative therapies for osteoporosis. Investigating the osteogenic potential of CQ, our research group previously reported osteogenic stimulatory effects of CQ (hexane fraction). The present study explores anti-resorptive effects of CQ-H on bones. We utilized RAW264.7 murine macrophage cell line as o...
Qiangsheng Wu1, Yumei Yin2, Hui Gao1, Baoding Chen3, Jianjun Yu2, Jian Zhang4, Donglai Zhu5, Fang Liu6, Guohong Ge7 and Ya-Mei Dang8*
...hepatocellular carcinoma patients’ tumor tissues were compared to 8 normal liver tissues to screen out the significant abnormal expressed genes. The potential interactions and mechanisms of these genes in promoting hepatocellular carcinoma were analyzed through bioinformatics analysis. The results showed that the functions of these 1495 differently expressed genes enriched in cell cycle, oxidation-reduction and drug metabolic process. Additionally, the t...
SADAF IFTIKHAR1, SAMAN SHAHID2*, MUHAMMAD UMAR HASSAN1 & UBAID ULLAH3
...ical cases. 141 COVID-19 patients of severe and critical categories, were enrolled from an epicenter tertiary care hospital. The mean values of platelets were found normal, whereas, the mean values of LDH were found elevated in both severe and critical cases. There was no patient with low value of LDH in both groups. 87% patients were having high values of LDH in severe cases, whereas, 98% patients
Muhammad Ashfaq ur Rahman1*, Saleem Khan1, Aurang Zeb1, Zia ud Din1 and Zafar Iqbal3
...unseling. A total of 150 patients were enlisted for this prospective analytic case-control (case-cohort) double-blind, randomized feeding experiment, with each group receiving 15 participants on average. Nutritional counseling sessions were provided to patients in the nutrition therapy groups in order to urge them to follow one of nine pre-tailored diets designed to meet the nutrient needs of the group. They were also given ...
Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*
...ofiling of IL-17A in HCV patients from Pakistan. Thus, we investigated the relationship between serum levels of IL-17A and age in chronic and fibrotic HCVpatients by ELISA and evaluated different markers that are invloved in the progression of CLDs towards HCC by performing histopathology and immunohistochemistry (IHC) techniques in liver tissues in HCV patients from the southern districts...
Qi Jiayi1, Huang XuMiao2, Lin Wei3, Ao YaHui3, Zhao Wenting2* and Cao Chunhao3*
...rvival analysis results, patients with high-risk scores had poor survival outcomes. To conclude, we constructed a predictive signature of four immune-related lncRNAs, which can provide assistance for prognosis prediction and immunotherapy of SKCM....
Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*
...mical parameters in PCOS patients with that of normal females including correlation between interleukin-3 and blood cell count. Patients with PCOS and normal healthy females (n=60) of same age group were included in this study following Rotterdam ESHRE/ASRM criteria. The parameters evaluated were; white and red blood cells and platelets figure, lymphocytes, neutrophils, monocytes and eosinophils percentage and serum level of...
Zhi Qiang Gao*, Dan Dan Zhang, Qin Gmei Qin, Xiu Xiu Li, Li Li, Yan Zhong Xue and Shifeng Guo
...s were collected from 40 patients diagnosed with glioneuronal tumors (GNTs), including 25 cases of gangliogliomas (GGs) and 15 cases of dysembryoplastic neuroepithelial tumors (DNTs). Then, the DNA of GNTs tissues was extracted, and the PSEN1 E280A mutation was detected by sequencing. Finally, the correlation of PSEN1 E280A mutation with clinical characteristics was analyzed. PSEN1 E280A mutation was detected in 16 GNTs patients...
Qingsen Ran1,2, Manjing Li3, Jiayin Han3, Lifang Wang3, Han Wang3,4, Shaobo Liu5* and Yanping Wang1*
...survival (OS) rate of CC patients through gene set enrichment analysis (GSEA) and Cox regression models. The K-M method was used to determine the difference of OS rate with high and low risk scores. The accuracy of risk scores were determined based on receiver operating characteristic (ROC) curve. Hierarchical analysis and Cox regression were performed to assess the correlation between CC risk score and clinical symptoms. Moreover, qPCR was used to verify the ...
Muhammad Javed Iqbal1, Farah Rauf Shakoori1*, Bushra Muneer2 and Abdul Rauf Shakoori3
... were explored in female patients with breast cancer. The demographic studies showed that all the patients were married, housewives with an average age of 47 years. The invasive ductal and invasive lobular carcinoma were detected in 85% and 15% of cases of all breast cancer patients. These tumors were classified into G1, G2 and G3 grades with an incidence of 12.5%, 42.5% and 45%, and group...
Shehar Bano Bhatti1, Muhammad Irfan Malik2, Rabia Faryad Khan1, Mahmood Ul Hassan2, Muhammad Shahbaz Aslam3 and Zaigham Abbas1*
...level was high in asthma patients (Mean = 508.7 IU/ml). In conclusion, this study suggested the association of atopic asthma with HLA DRB1 alleles in the Pakistani population of the Punjab region. Our study reveals that two HLA DRB1 alleles have a positive, and two alleles have a negative association with atopic asthma in the Pakistani population. This finding emphasized that further large-scale studies are needed in the Pakistani population to determine the a...
Zhimin Wu1,2, Yaping Yang1, Arshad Zahoor1, Aftab Shaukat1 and Ganzhen Deng1,2*
...parameter threshold, and patients with respiratory diseases and obvious clinical syndromes (n=57) were selected as trials to evaluate and confirm the availability of these indexes for the diagnosis of canine pulmonary function. The most valuable indexes for the clinical diagnosis of abnormal pulmonary function were selected with correlation analysis and single factor analysis of variance. The posterior lung field angles and the thoracic indexes showed no corre...
Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*
... grade of clear cell RCC patients were analysed. The classification using ISUP was significantly correlated with the expression of EPAS-1 (P < 0.05). The ROC curve showed that the high expression of EPAS-1 has a certain diagnostic value for lymph node metastasis in RCC (P < 0.01). To conclude The high expression of EPAS-1 is closely related to the adverse clinicopathological characteristics of RCC and can be used as a potential therapeutic target for thi...
Kanwal Nisa1, Sadia Roshan1, Shazia Shamas1,2*, Raheela Atta Mustafa3, Shamaila Irum1, Kalsoom Sughra4 and Memoona Iqbal1
...one with prostate cancer patients by the evaluation of a tumor marker Prostate-specific antigen (PSA). Prevalence was recorded in Gujrat, Punjab, Pakistan. Blood samples of normal and prostate cancer males were taken from Gujrat, Punjab, Pakistan. Information including age, marital status, smoking, Prostate-specific antigen (PSA), testosterone, and any previous record was obtained with the patient’s consent. Exclusion and inclusion criteria were defined....
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
...ized. Wounds in diabetic patients are difficult to heal. Green synthesis is one of many methods that can be used to synthesize silver nanoparticles. Based on a statistical analysis of the average wound area, epidermal reconstruction, and TIME-H scores, significant differences began to occur from day 12 to day 14. The average wound area, epidermal reconstruction, and TIME-H scores in the diabetic mice group that were given a dermal patch formulated with silver ...
Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni
...s were selected from out patients of Center of Cardiac and Digestive System, Sohag, Egypt aged from 20 to 55 years. Then, confirmed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) for HCV. The confirmed patients were received 12 vials of BEG FN- 2a for 12 weeks (1801U/ml weekly) plus ribavirin (1000 mg for 75 Kg or 1200 mg for > 75Kg- Roche) and follow up by RT-PCR. The results showed that 67
Elkalamawyl, I.M.; Elhddadl, S.; Swelim2, M.A.; Hamdy2, S.M. and Fahmy3, Hanan A.•
...olated from asymptomatic patients who were found to be persistently positive for both I-BsAg and anti-hepatitis B surface antibody (anti-HBs) and a long-lasting anti-HBc (core) IgM. Polymerase chain reaction (PCR) and sequencing were performed to surface antigen region and revealed two genotype D mutants 1--1BV. Aligned with known I-BsAg sequences from GenBank, the mutant variants matched to consensus with eight distinct genotypes (A to H) of hepatitis B virus...
Iftikhar Alam1*, Sumbal Ali1, Anwar Ali Shad2, Aneela Tahmeed1, Hafsa Nawaz1, Shumail Bakht1 and Muhammad Farooq3*
...ehavioral changes in the patients to achieve health targets. There are many challenges one may face while developing a story for health communication. A big challenge usually faced by a health story writer or developer while compiling the story maybe being evidence-based vs. staying true to the story. While understanding literary elements are essential for a health story and while there are numerous tools available to evaluate the scientific evidence presented...
Fadia M. Attia and Hanaa H. A. Gomaa
...logical abnormalities in patients with chronic hepatitis C. Aim of the study: to describe different patterns of changes accompanying viremic status in HCV chronic liver diseases. 50 patients with chronic hepatitis C were enrolled in this study. Qualitative and quantitative PCR by real time PCR was done to determine the level of viremia before induction of therapy. In addition, complete blood count, prothrombin time and liver...
Mazharul A. M. 1, Md. S. Sarwar ,M. Nurullah 2, Keshab K. Adhikary I and M. Rahmatullah 1
...ocera were used to treat patients with rabies.
... 
Fadime Eroglu1*, Altan Akineden2, Cemal Cicek3, Selcuk Turkel2
...ifficulties and death of patients due to symptoms overlapping with COVID-19 disease. It was aimed to investigate the distribution of intestinal protozoan infections in COVID-19 patients and the relationship between these protozoans with clinical symptoms of COVID-19 disease. COVID-19 patients included in the study were classified according to their clinical symptoms as mild, moderate and s...
Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*
...f blood glucose level of patients with type II diabetes mellitus and coronary heart disease (CHD) on the hypercoagulability and thromboembolism. A total of 236 patients with type II diabetes mellitus and CHD who were treated in 904 Hospital of Joint Logistic Support Force of PLA were enrolled between January 2018 and January 2020. These patients, according to their 2 h postprandial glucose...
Qing Cao1, Wei Jiao2, Huihui Lu1, Jing Zhang2, Meiling Ren3, Yan Xu1* and Shuyang Hu1*
...f blood glucose level of patients with type II diabetes mellitus and coronary heart disease (CHD) on the hypercoagulability and thromboembolism. A total of 236 patients with type II diabetes mellitus and CHD who were treated in 904 Hospital of Joint Logistic Support Force of PLA were enrolled between January 2018 and January 2020. These patients, according to their 2 h postprandial glucose...
Rehana Shahida1, Shagufta Naz1*, Tasnim Farasat1, Saima Sharif1, Shah Jahan2 and Farkhanda Manzoor1
...n vascular damage in the patients having impaired glucose tolerance (IGT) and to identify the relationship of IL-6 with insulin resistance. The patients visiting Amin Hayat Memorial Diabetic Center, Lahore from (January 2017 to October 2017) the subjects following WHO criteria of IGT and Diabetes were included. Demographic parameters as age, body mass index, waist/hip ratio, blood pressure, and socioeconomic status were reco...
Genlong Zhan1*, Yunhua Zheng2, Desheng Li*4 and Hongmei Zhang3
...xue Tongmai Decoction on patients with diabetic peripheral neuropathy (DPN). A total of 130 patients with DPN who were treated in our hospital from January 2015 to July 2016 were selected and randomly divided into a control group and a treatment group, with 65 cases in each group. The control group was treated with conventional western medicine, and the treatment group was treated with self-made Yiqi Huoxue Tongmai Decoction...
Nusrat Bano1, Muhammad Tayyab1*, Bushra Muneer2, Sehrish Firyal1, Abu Saeed Hashmi3, Muhammad Wasim1 and Ali Raza Awan1
...cal parameters in dengue patients for the quick diagnosis and detection of dengue stage for the proper treatment of infection. This cross-sectional study was conducted at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore and Jinnah Hospital Lahore (JHL) from January to December 2013. Total 345 clinically suspected patients reporting to JHL were serologically diagnosed for dengu...
IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3
... infected burned skin of patients in children hospital, Lahore, Pakistan. The isolated microorganisms were identified as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were soaked in three different concentrations (50%, 70% & 90%) of each of the three unifloral honey samples for 48 hrs. The filter discs ...
SHAGUFTA ANDLEEB1*, ASMATULLAH2, SHABANA SHAUKAT1 & SIDRA NAWAZ2
...lycemic drug used by the patients of diabetes all over the world. In present study, Metformin hydrochloride (Glucophage) was used to determine the histological changes in liver of mice. Twenty mice (4-5 weeks old) were given oral gavages of 0.1 ml of 0.00, 31.25, 125 and 500μg/g B.W (Complete). of Metformin prepared in distilled water, for 04 weeks daily. After 24 hours of last dose, the animals were anaesthetized torecoverliver tissues following fixation i...
ASIFA BASHIR1, AAMIR MUSHTAQ2* & TOOBA MEHBOOB3
...c agent in hyperglycemic patients. Ethanolic extract of its dried fruits was analyzed both qualitatively and quantitatively for evaluation of phytochemical agents. Then its antioxidant and anti-diabetic activities were found. The results indicated the presence of carbohydrates, tannins, phenols, alkaloids, flavonoids, saponins, glycosides, amino acids and proteins in crude extract with phenolic and flavonoid contents observed as 9.9 mg/g equivalent of gallic a...
SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1
...der and social status of patients was determined. Results of the study revealed that 61% of samples were positive for Escherichia coli, other contributing pathogens were Staphylococcus spp. (24%), Klebsiella spp. (9%), Proteus spp.(4%) and others (2%). Out of 85 samples (march-june 2015), 48(56%) were from females and 37(44%) from males. Samples were categorized into five age categories age and majority of the collected samples (42%) were found in age group of...
ASMA ZAFAR*, MONIBA KHAWAR, MAHNOOR CHAUDHRY, SAYYEDA SANA BATOOL, AMJAD HUSSAIN** & MUSHTAQ AHMAD SALEEM
...de effects to the cancer patients, as they not only target cancer cells but also cause harm to normal body cells. At present some live bacteria, bacterial products, and bacterial proteins are used to treat cancer. One of the amazing bacterial protein azurin can work as an anti-cancerous agent which only target cancerous cells and do not cause any harm to healthy cells and thus minimize the risk of adverse side effects. The interaction of azurin with P53 (tumor...
KOUSAR PERVEEN1*, MUHAMMAD AFZAL1, SANA SEHAR1 & SYED AMIR GILANI2
...cal facilities may endow patients‟ physical health but patients‟ emotional well-being depends on how healthcare personnel treat patients during the curing process. In this regard, the extant literature suggests that compassionate care behaviour of healthcare personnel plays the leading role. However, scant attention has been paid to understand the phenomena of compassionate care in dev...
ZAHRA NASEEM1, MUHAMMAD AMIR IQBAL1, SHAAF AHMAD2 & NABILA ROOHI1*
...y five overt hypothyroid patients was also carried out after taking thyroxine for three months. Serum levels of fT4, fT3 and TSH were analyzed through Radioimmunoassay (RIA), whereas, levels of AST, ALT and creatinine were assessed using chemistry analyzer. Levels of ALT and AST were found significantly increased (P ≤ 0.05) in overt hypothyroid patients compared to controls and after treatment the concentrations of both e...
MUHAMMAD NAEEM RAZA1, KALSOOM SUGHRA1* & NADIA ZEESHAN1
...f 3540 (77.6%) suspected patients were positive for HCV-RNA. Female have higher percentage (57.78%) than male (42.22%) with highest viral load observed at age group 15 to 30 years. The genotype 3a (83.33%) was dominant to other circulating genotypes as 2b (13.33%) and mixed 3a/2b (3.33%). The highest prevalence of HCV infection was noted in females compared to males and 3a was most dominant genotype. This study highlights the new reported mixed genotypes 3a/2b...
UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1
...cted blood samples of 70 patients out of which 50 ladies gave untimely births and 20 ladies gave typical births. DNA extraction technique was applied. Primers were designed, amplified by PCR and RFLP were performed to identify the TNF–alpha -308 and -238 polymorphisms. The frequencies of TNF-alpha -308 genotype TNF GG, GA and AA were 37%, 10% and 3% with measurably estimation of 0.07039 (P<0.05) as indicated by HWE for the preterm and postterm conveya...
Aliya Irshad Sani1, Shumaila Usman2*, Zile Rubab1, Sufyan Ahmed3, Sana Iqbal3 and Zehra Ahmed4
... squamous cell carcinoma patients in saliva and blood as noninvasive biological samples by qPCR. The results revealed significant difference between the cycle threshold (Ct) values in the saliva and blood of OX40 (p value=<0.001) and OX40L (p value=<0.001) in OSCC patients. The fold change overexpression in blood and saliva is also compared in relation to histological gradings and clinical staging. Based on our observa...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
.... sativa and honey in MS patients....
Muhammad Usman1, Majid S. Hashmi1, Ayaz Ahmad1*, Fawad Ahmad2 and Zahid Alam1
...s generally and diabetes patients particularly. Moreover, some low-calorie products did not meet sensory preferences of consumers. Therefore, this research was conducted to evaluate the quality and acceptability of a low-caloric orange drink by incorporating various concentrations of stevia powder and storing it within a temperature range of 20±0.4°C. The investigated treatments included: Orange Drink (OD)0 (Juice: 15%, Sugar: 13%, Stevia: 0%, Sodiu...
Jin Jiao1, Jin Song1, Yuanyuan Cui1, Yilin Wang2, Yanling Li1, Jing Li1, Yan Su1, Xiaojing Du3, Yan Wang3, Guiping Sun3 and Minxiang Wei4*
...nxiety and depression in patients with myocardial infarction (MI) and analyse the relationship between fatigue and sleep disturbance, anxiety and depression. A cross-sectional research method was used to recruit 190 patients who were at least one-week post-MI as the research participants. The Pittsburgh Sleep Quality Index, the Chinese version of the Multidimensional Fatigue Scale and the Hospital Anxiety and Depression Scal...
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