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Timothy Helton

In a Mirror Dimly: Anthropology and Restoring a Sense of Presence to an Empty World
...ude by suggesting the collaboration between anthropology and theology in the restoration of a world capable of supporting life in more than a mere physical sense.

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Mark Zelcer

...s. Though for reasons I elaborate on later, I would not recommend this to experts in those fields, but rather to non-professionals.

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Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3

...inical, pathological and laboratory findings of this outbreak. The disease was clinically manifested by the depression, diarrhea, difficult breathing, muco-purulent ocular-nasal discharges with matted eyelids and encrusted nostrils and finally death. Clinical outcome, gross lesions and histological observations were suggestive of PPRV infection, which were confirmed by the application of a reverse transcription–polymerase chain reaction (qRT-PCR). Collec...

Dana Kay Nelkin

... to challenges serve to elaborate, extend, and deepen the view. The result is a book that, along with the first, will be a centerpiece of the conversation about the metaphysics of free will, about morality, about moral responsibility and our related emotions and practices, about the pressing practical and moral issues concerning punishment, and about meaning in life. Finally, the book itself is a wonderful manifestation of a commitment to certain intellectua...

Muhammad Ashraf Khan1, Hizbullah Khan1, Abid Farid2

...es were conducted in the laboratory to assess the effects of Helicoverpa armigera nu- cleopolyhedrovirus (HaNPV) applied to eggs of Sitotroga cerealella Olive, on emergence of and para- sitism by female Trichogramma chilonis (Ishii) emerged from HaNPV- treated host eggs. The percent emergence (mean) of tiny parasitoid from host eggs treated during egg, larval and pupal stages with the virus at field dose (x), 2x dose, and 0.5x dose ranged from 83.2- 87.3%, 87....

Hayat Badshah1*, Farman Ullah1, Abid Farid2, Ahmad-U-Rahman Saljoqi1, Sajjad Ahmad3

...g of P. solenopsis under laboratory conditions for the mass scale production of Aenasius bambawalei. However, further study is suggested to identify if there are any detrimental effects on the reared parasitoid i.e. a tri-trophic effects on parasitoid- Aenasius bambawalei.

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Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3

...of Atteva sciodoxa under laboratory conditions of 27±2ºC, 75% RH and 12:12 h photoperiod. Instar 3rd responded with 100% mortality at 5x107 conidia ml-1, while same level of mortality was recorded in 5th instar at 1x108 conidia ml-1. Instar 5 also showed the lowest mortality (33.3%) at 1x106 conidia ml-1. Median effective concentration (EC50) ranged between 9.87x105 and 21.30x105 conidia ml-1 for 3rd to 5th instars. Median effective time (ET50) ra...

Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6

...istently infected with a laboratory strain of BDV (C6BV ) to establish the assay. Next, in situ PLA was applied to detect BDV P in brain tissues of infected animals. Finally, protein-protein interactions were visualized in both C6BV and brain tissues of experimentally as well as naturally infected animals (rat and horse, respectively). BDV proteins and their interactions with host proteins could be shown in cell cultures (HMGB1, Cdc2) and in brain tissues of r...

Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5

...CytB). Twelve samples of laboratory-reared population of each species were used for the study. Sequencing data were used to calculate haplotype, haplotype diversity and nucleotide diversity for the two species. Based on combined loci of cytochrome oxidase II (COII) and cytochrome b (CytB), twelve haplotypes were generated for G. p. palpalis and five for G. m. submorsitans. Similarly, for the combined loci, haplotype diversity (Hd) was higher for ...

Muhammad Khurshid, Muhammad Nafees, Inam-ur-Rahim and Wajid Rashid

...s of gender-based family labor distribution during past 30 years shows that herding labor (male and female) of age 14 to 60 significantly (P<0.01) reduced during past 30 years and partly shifted to cropping. The male herding labour reduced 40.2% in semi-nomadic pastoralists and 8% in nomadic pastoralists. Similarly the female herding labour reduced 54.3% in semi-nomadic and 32.2 in nomadic pastoralists. This reduction is ...

Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek

...vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more effective against M. hapla whereas S. rugosoannulata and C. rosea showed better results among other tested fungi in experiments. Moreover, plant growth parameters were also improved ...

Ai Ming Zhou1,2, Guang Wen Liang2, Ling Zeng2, Yong Yue Lu2 and Yi Juan Xu2*

Zaheer Ahmad Qureshi and Khuram Nawaz Sadozai

...he results were further elaborated through deciles under both income and expenditure approaches. The lower poorest category estimated through deciles contributes 2.78% to the overall rural region’s income. However, the upper richest category shares 30.06% to the aforementioned income. The deciles measured through expenditure approach revealed that the lower poorest category and upper richest category contributes 3.13 and 25.59, respectively. The study co...

Muhammad Zahid1, Muhammad Hamid Bashir1*, Bilal Saeed Khan1 and Muhammad Shahid2

...rations under controlled laboratory conditions. The results showed 25%, 30%, 70%, 50% and 65% mortality occurred by tested pesticides at field relevant doses after 144 h respectively. Missing mites data indicated repellency due to pesticides, was highest at the start of experiment, and then these acclimatized on leaf discs. Thiacloprid having lowest LC50 value 295 and proved toxic for the predator while diafenthiuron and pyriproxyfen have higher values. The fi...

Muhammad Imran*, Munir Ahmad, Muhammad Naeem, Muhammad Farooq Nasir, Imran Bodlah, Muhammad Nasir and Umer Ayyaz Aslam Sheikh

... stages under controlled laboratory conditions. Queen oviposition rate was 1.4-fold higher in polycarbonate box compared to wooden and cardboard materials. Early start of egg-laying (first oviposition period) and earlier emergence of first worker were found in polycarbonate box as compared to others. Similarly, colony foundation stage in polycarbonate box took 58.3 days which was about 7-10 days shorter than others box types. Early emergence of sexual were obs...

Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek

...s pest in sugar beets in laboratory and greenhouse trials. The fungi chosen were Arthrobotrys oligospora, Dactylella oviparasitica, Clonostachys rosea, Stropharia rugosoannulata, and Lecanicillium muscarium. In the laboratory experiment, S. rugosoannulata proved to be the most efficient biocontrol agent by parasitizing the maximum number of eggs, whereas D. oviparasitica appeared to be the least efficient after 72 hours...

Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A

...ricius was harvested for laboratory investigation using AGID for infectious bursal disease virus (IBDV). As part of investigation to screen for co-infection of CIA and IBD in the flock, the BF was also screened for the presence of chicken anemia virus (CAV) using conventional polymerase chain reaction (PCR). IBDV antigen was detected by AGID while CAV by PCR. This confirms the diagnosis of concurrent infection of CIA and IBD in the affected flock. This is the ...

Muhammad Sohail1,*, Muhammad Naeem Khan1, Naureen Aziz Qureshi2 and Abdul Shakoor Chaudhry3

... + Cu) for 15 days under laboratory conditions. Gill cells of mussels were used to determine the DNA damage by comet assay. The tail DNA (%), comet tail length and olive tail moment (OTM) were the parameters selected to detect DNA damage. Low doses (120 µg L-1) of each metal induced significantly higher levels of DNA strands breaks as compared to medium dose (240 µg L-1) and very low levels of DNA damaged was observed at high dose (360 µg L-1...

Marryam Bakhtawar1, Qamar Saeed1* and Naeem Iqbal2

...a undecimpunctata, under laboratory conditions was done. These include mustard aphids (Lipaphis erysimi) cotton mealybug (Phenacoccus solenopsis), grain moth eggs (Sitotroga cerealella) and honey. Results revealed complete development of all larval stages on all diets with maximum survival of lady bird beetle larvae on mustard aphid (76.6%) followed by on grain moth’s eggs (20%), immature mealybugs (6%) and the least survival on honey. These findings can...

Neelum Andaleeb and Munir Khan

...f total cost followed by labor cost. Average milk produced by buffalo and cow was 180 and 135 liters per month, respectively. Average total costs of milk production of buffalo and cow were Rs.12835 and Rs.10070 per month respectively whereas the average net income per liter from the buffalo and cow milk was Rs. 29 and Rs. 5 thus benefit-cost ratios for buffalo and cow is1:1.43 and 1:1.07, respectively, which show buffaloes were more productive and profitable t...

Ibni Amin Khalil* and Raziuddin

...und much cooperative in elaborating the genetic parameters of the data on the basis of its high explanatory aptitude and graphical demonstration.

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Muhammad Arshad1*, Hafiz Azhar Ali Khan2*, Faisal Hafeez3, Ravaid Sherazi1 and Naeem Iqbal4
...is) was evaluated in the laboratory in no choice and free choice feeding assays. In the no choice feeding assay, the stages of beetle (adults, 3rd and 4th instar) consumed more aphids than early stages (1st and 2nd instar). In the free choice feeding assay, the consumption of pea aphid (77.647) was statistically high followed by spinach (66.276), coriander (66.14) and cabbage (61.48) aphids. Manly’s preference ...
Abdur Rahim1, Ghulam Abbas1,*, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6
...e brought to aquaculture laboratory of the Centre for 15 days acclimatization. After acclimatization, they were randomly distributed into the rectangular tanks (3 × 1.5 ×1.5 ft each). In each tank, 10 fish were stocked with three replications for each treatment. Four isonitrogenous diets containing different oils i.e., fish oil (FO), soybean oil (SO), olive oil (OO) and palm oil (PO) were given to the juveniles for 60 days. Best specific gro...
Naime Zülâl Elekcioğlu*
...ordm;C increments in the laboratory. Developmental periods of immature stages ranged from 34.98 days at 15ºC to 9.15 days at 35ºC. The lower developmental threshold for C. phyllocnistoides estimated was 8.93°C. Mortality rate of immature stages decreased as the temperature increased, ranged from 27% at 15ºC to 8% at 35°C. Longevity of both sexes were different at all temperatures studied (except 30 and 32.5°C), ranged from 22....
Muhammad Afzal1,Naveeda Akhtar Qureshi1,*, Khalid Zamir Rasib2 and Irfan Hussain1
...e assays conducted under laboratory and field conditions. Visual rating according to (AWPA, 1997) scale, mass loss, wood consumption per termite and mortality rate were determined to evaluate resistance of wood against H. indicola. Populus euramericana and Dalbergia sissoo were observed most palatable and resistant wood species, respectively, in no-choice laboratory and field trials. Five different woode...

 Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy

...rdance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds all four pathogens disseminated in various organs with higher incidence of mixed compared to single infection. Different serotypes of E.coli and Salmonella could be recovered from dead birds however E.coli (O78) and S.enteritidis were recovered mainly from heart and liver. The recovered...
Muhammad Tahirand Memoona Shehzadi*
...biology and biochemistry laboratory, Institute of Soil and Environmental Sciences (ISES), University of Agriculture Faisalabad. In this experiment Randomize Complete Block Design (RCBD) with factorial arrangements having three replications was used. Significantly maximum 1000 achene weight, achene yield, biological yield, harvest index and protein content were recorded where seed inoculation with PGPR-1 + PGPR-2 and full dose of recommended chemical was applie...

Muhammad Khurshid1,2*, Muhammad Nafees1, Mehmet Somuncu2

...egion. Integrated and collaborative approaches are needed for pasture resources management with efficient and sustainable livestock production in Northern Pakistan.

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Said Rawan1, Farzana bibi1, Nadeem Khan1*, Abdul Mateen Khattak1, Zahir shah3, Asif Iqbal2, Madeeha Alamzeb2, Saeed Ul Haq1, Abid Kamal3, Fawad Ali Shah2, Ahmad Naeem1 and Wajid Ali3
...onducted at post harvest laboratory, Department of Horticulture, The University of Agriculture Peshawar, during the year 2015. The research work was conducted in Complete Randomized Design (CRD) with three replications and two factors i.e. guava varieties (Safeda and Karela) and storage intervals (0, 3, 6, and 9 days). The data was recorded onfirmness (kg/cm2), weight loss (%), moisture contents (%), taste, decay fruits (%) and total soluble solids ...

S. K. Mukhopadhyay and M. V. Santha Kumar

Studies on the biosafety of botanical insecticides to native natural enemies of mulberry ecosystem

A. K. Chaubey and Satyandra Kumar

Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...lycopersici on tomato in laboratory and pot culture experiment. All the isolates were isolated by serial dilution and cultured in liquid protein supplemented broth medium and culture filtrates (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isolates in PDA media plates. In in-vivo test soil wa...

K.N. Ahmed and M.R. Hasan

Sudden outbreak of mealy bug and armoured scale causing severe damage to economic crops in Bangladesh
...gous in nature. Based on laboratory studies on immature and mature fruits of lady's finger; the total period of all four nymphal stages lasted for 32.6 ± 4.17 days at an average room temperature of 28 ± 2ºC and 70-75% RH during September, 2009. Male mealybug has a short life span of 3.0 ± 0.21 days. Females live for nearly 80.7 ± 10.43 days with an oviposition period of approximately 21 days. This mealybug is capable of active ...

Sitansu Pan and Amrita Das

Population proliferation and spread of Trichoderma spp. in soil under two different delivery systems
...) was studied both under laboratory and field conditions following two types of delivery systems, through organic based (vermicompost + neem cake (90% + 10% w/w)) and seed based (sterilized wheat seed) inocula. The population proliferation and spread of the antagonists were faster when they were applied through organic based inoculums over that of seed based inoculums from the point of application. Similarly population proliferation and spread of the antagonis...

Nilesh Suresh Gole and Bijan Kumar Das

Biology of Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera), a serious pest of Indian ipecac, Tylophora indica
...nducted in the field and laboratory of All India Coordinated Research Project on Medicinal & Aromatic Plants and Betelvine (AICRP on MAPB) at Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, Nadia, West Bengal during 2010-2011 on seasonality, extent of damage and biologoy of D. sagitta on T. indica. The larvae were active almost throughout the year, fed voraciously on leaves and flowers causing extensive damage to T. indica during June and December. E...

SK Fashi Alam, Atanu Seni and Ajoy K Sahoo

Biology of the mealybug, Phenacoccus solenopsis Tinsley (Pseudococcidae: Hemiptera) on sprouted potato and brinjal plant
...ted potato tubers in the laboratory. The female nymphs moulted three times for becoming adults while the male took four moultings. The female and male nymphs completed development in 14.45± 0.96 days and 19.53±1 days, respectively on brinjal plant at 20-36.2° C temperature and 15.1±1 days and 18±0.82 days respectively on sprouted potato. The ratio of the male: female were 1:1.75 and 1:1.87. The duration from nymphs to adult fema...

Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
Muzammil Sattar
... proteins were tested in laboratory at four concentrations, i.e., 1, 3, 5 and 7 mg/ml in diet. Significantly higher fecundity 785.12±25.7, fertility 89.23±0.36 % of eggs and longer oviposition period 42.13±1.11 days were recorded in adults feeding on a diet containing Nu lure followed by protein hydrolysate (control diet). The F1 progeny larvae that developed from adults consuming (Nu lure 5ml) diet had the shortest larva...

Naveed Muhammad, Syed Attaullah Shah*, Abbas Ullah Jan, Irfan Ullah, Muhammad Ibrahim and Salman Khan

...achinery, seed quantity, labor, number of irrigation, chemicals spray and fertilizers have positive significant effects on tomato yield. Allocative efficiency analysis reveals that machinery, seed quantity, number of irrigation, fertilizers and chemicals are underutilized, while labor and FYM are over utilized for tomato production in the study area. The study recommends training for growers and field <...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...tera litura F. under laboratory conditions. Both NPV and flubendiamide were applied using diet incorporation method. Larvae were allowed to feed for 48 h. Mortality was recorded until larvae died or pupated. Second instar larvae showed more susceptibility as compared with 4th instar larvae. Maximum mortality was recorded in 2nd instar larvae (91.02±2.04%) in comparison to fourth instar larvae (69.35±1.66%) in combined ap...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...H. armigera under laboratory studies for toxicity, sublethal and antifeedant effect. The result showed that ethanol based extract was the most effective to control the H. armigera followed by the ethyl acetate. The sublethal concentration of ethanol based extract of C. colocynthis increased larval and pupal duration as compared with control. The percent pupation, adult emergence and pupal weight decreased in treated populations as c...

Haidar Ali* and Malik Muhammad Shafi 

...e large number of family labors of sample respondents were working on high paid jobs or employed else or self-employed and thus contributed a good amount to their family’s income. The study recommends that unemployment in the agriculture sector is likely to increase with the decrease in the size of holdings. To generate off-farm employment opportunities agro based industries seems to potential area. Simultaneously there is need for initiating skill devel...
Qunhua Han1,2, Meiwen Zhang1,*, Cong Guo2, Xunjun Zhou1, Bo Li1 and Yong Wang1
Hayat Badshah1, Farman Ullah1, Paul-André Calatayud2, Hidayat Ullah3 and Bashir Ahmad1
...stigated under field and laboratory conditions the influence of the host plant of P. solenopsis on the parasitism success and the female fitness of A. bambawalei, Five plant species, commonly found to be host of P. solenopsis, were tested: hibiscus, potato, okra, eggplant and tomato. Under no choice test conditions, the results showed that the % parasitism did not differ significantly between different host plants in ...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...ted under greenhouse and laboratory conditions. A sharp reduction in the population of T. urticae was obtained after one week of applying Huwa-San TR50 at a rate of 4000 ppm. This resulted in mortalities of 82.10 and 78.60% under greenhouse and laboratory conditions, respectively. The side effects of Huwa-San TR50 on predatory mite, Neosiulus cucumeris, were also tested to gain successful implementation of new ...
Saleh S. Alhewairini
...was evaluated under both laboratory and field conditions. In the laboratory, oxamyl had significant effects on the mortality of RPW. After exposure to direct spray of oxamyl, adult mortality was 62, 82 and 100% whereas larvae mortality was 72, 77 and 100% after 1, 24 and 48 h, respectively. Alive adult and larvae were completely paralyzed after 24 hours. There was significant difference between 2 treatments, 1 and 24 h, in b...
Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
...40) mixtures under laboratory and field conditions (71.5 ± 7.4). The results showed a significant effect of binary treatments of fungi and chemical insecticides on biological parameters of C. pipiens and its progeny (P<0.05). The insect pathogenic fungi showed compatibility with insecticides and the combined application can improve the management program of C. pipiens.
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Faiza Ambreen*1 and Muhammad Javed2
... for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for the DNA damage assessment in-terms of percentage of damaged cells. In general, a dose dependent response was observed in fish erythrocytes with induction of maximum DNA damage at highest concentration (1/3rd of LC50) of pesticide mixture. Statistically significant effects for both concentrations and time of...

Naila Riaz1 , Hafiz Muhammad Tahir2* , Khizar Samiullah3

...ptera: Aphididae) in the laboratory. R. erysimi were treated orally as well as topically with different concentrations of the crude venom. All aphids died within 6 hr with oral treatment at 1.5µl of the venom dose. However, at the same venom dose, 100% mortality was achieved at 8 hr posttreatment when applied topically. Similarly, oral administration of 1µl and 0.5µl venom caused 100% mortality at 8 hr post-treatment compared to 10 hr and 12 ...

Muhammad Tariq1*, Muhammad Khawar Hussain1, Zilakat Khan Malik2 and Noor Jehan1 

...aration, NPK fertilizer, labor, seedling, farm yard manure and pesticides were recorded 6.00 hrs, 248 kgs, 143 man days, 24413 seedling, 1.48 trollies and 1.21 litres respectively. The results also showed that strawberry producers were having 81% of mean technical efficiency with minimum to maximum technical efficiency limits of 0.42% to 0.98% respectively. Moreover out of 382 the technical efficiency of 178 farmers was more than 90%. Furthermore, farmers with...

Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain 

...samples were analyzed in laboratory for the presence of bacteria. The samples were crushed homogenized in 0.85% TBE buffer and platted on YDC (Yeast extract-Dextrose-CaCO3) for bacterial growth. For identification of the characteristic bacterial colonies Cmm-specific primers CMM-5 and CMM-6 were used for amplification of 614bp product from pat-1 gene of bacterial plasmid. The whole bacterial DNA was used as template, extracted with standard procedures using co...

Saima Akhtar Qureshi1 and Asim Anwar2*, Ather Maqsood Ahmed

...ulture departments to collaborate with international agencies to introduce sound agriculture practices like Integrated Pest Management (IPM) in Pakistan. In IPM technique natural parasites and predators are used to check pest growth instead of pesticides which reduces ecological and health damage. Pesticides are used at the last resort in IMP method so it reduces the cost of production and increases the farmers’ profit. However, concerns about production...

Fazal Ullah1*, Muhammad Sajid1, Syeda Leeda Gul2, Bibi Zainub1 and Maaz Khan

Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
... and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not promote microbial growth rather it has antibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is safe for consumption as no clinical symptoms were observed in rabbits after giving the maximum dose of 1000ml of color.
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Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
... in a routine diagnostic laboratory. High diversity values were found in the studied area implying high population diversity of M. tuberculosis that’s being prevalent in Khyber Pakhtunkhwa, Pakistan.
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Nazish Shaukat1, Muhammad Javed1,Faiza Ambreen2* and Fariha Latif1
...ent was conducted in the laboratory by exposing four groups of Labeo rohita to different doses of PbCl2 viz. 96-h, 2/3rd, 1/4th and 1/5th of LC50, separately, for 30 days and compared with control group. After 30 days, fish was sacrificed and their gills and liver isolated to determine the effect of PbCl2 on peroxidase activity. Activity of peroxidase enzyme in metal stressed fish ...

 Asrar Ali*, Ehsan-ul-Haq**, Abdul Rehman***, Javed Khan, Waseem Ahmad Gillani** and Maria Rauf*

BIOLOGICAL PARAMETERS AND PREDATORY POTENTIAL OF MENOCHILUS SEXMACULATUS FAB. (COLEOPTERA:COCCINELLIDAE) AT VARYING TEMPERATURE ON RHOPALOSIPHUM PADI L.

 Tahir Abbas, Bushra Nawab, Rahila Nazli, Rubina Saleem, Amrat Lal and Khalid Jamil*

EFFICACY OF COTTON WASTE COMPOST AND FERTINEMAKIL FERTILIZER ON THE GROWTH PARAMETER OF SUNFLOWER PLANTS

 Rizwana Sultan*, Javed Khan, Ehsan ul Haq, Tariq Mehmood**, Roshan Zada Khattak***, and Naheed Akhtar**

BIOLOGICAL PARAMETERS OF TRICHOGRAMMA CHILONIS ISHII (TRICHOGRAMMATIDAE: HYMENOPTERA) FEEDING ON SITOTROGA CEREALELLA EGGS AT THREE CONSTANT TEMPERATURES
...m parasitized eggs under laboratory condition of 28 ±1 °C.


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 Maria Rauf*, Ehsan-ul-Haq**, Javed Khan**, Abdul Rehman***, Waseem Ahmad Gillani* and Asrar Ali**

BIOLOGY AND PREDATORY POTENTIAL OF COCCINELLA SEPTEMPUNCTATA LINN. ON SCHIZAPHIS GRAMINUM APHID UNDER CONTROLLED CONDITIONS

 Muhammad Shahzad*, Akhter Ali**, Abdul Hayee Qureshi**, Naveed Jehan*, Imran Ullah* and Majid Khan*

ASSESSMENT OF POST-HARVEST LOSSES OF PLUM IN SWAT, PAKISTAN
...urity, using experienced labor and storage facility can reduce extent of post harvest losses of plum.

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 Shahida Khalid*and Shahida Nasreen Khokhar*

INTERACTION OF HERBICIDES AND BIO-INOCULANTS WITH AGRICULTURAL CROPS AND WEEDS
... The crops studied under laboratory conditions were Brassica spp. and Zea mays while Phalaris minor was the test weed. In first experiment, Brassica and maize, grown in soils having residues of either Triasulfuron+Terbutryn (Logran) or Sulfosulfuron (Leader), were found to significantly mitigate (% increase in root length, shoot length, fresh weight and dry weight) the inhibitory effect of residues , when inoculated with Azospirillum A4. In second experiment t...

 Muhammad Tayyab Mudassar*, Ehsan-ul-Haq*, Muhammad Naeemullah**, Muhammad Shakeel*, Muhammad Ashfaque*, Asmat un Nisa* and Javed Khan*

ECONOMIC REARING OF CHRYSOPERLA CARNEA (STEPHENS) (NEUROPTERA: CHRYSOPIDAE) IN INSECTARY
...oduction of C. carnea in laboratory using available and inexpensive equipment to produce the healthy surplus of this predator for experiment and small holding field releases, S. cereallela is recommended as more efficient and economic host as compared to P. solenopsis.

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 Javed Khan*, Ehsan-ul-Haq*, Habib Iqbal Javed*, Tariq Mahmood*,
Awais Rasool*, Naheed Akhtar and Saleem Abid**

BIOLOGICAL PARAMETERS AND PREDATORY POTENTIAL OF CHRYSOPERLA CARNEA (NEUROPTERA: CHRYSOPIDAE) FEEDING ON WHEAT APHID SCHIZAPHIS GRAMINUM (HEMIPTERA: APHIDIDAE) UNDER LABORATORY CONDITIONS

 Naveed Jehan*, Khalid Mahmood Aujla**, Muhammad Shahzad*, Abid Hussain**, Muhammad Zahoor*, Majid Khan* and Ahmed Bilal*

USE OF MOBILE PHONES BY FARMING COMMUNITY AND ITS IMPACT ON VEGETABLE PRODUCTIVITY
...range transportation and labor services in time. The study was conducted to see the effect of timely information availability with mobile on productivity. The data was collected through well structured questionnaire by interviewing 60 farmers from district Charsadda. The data was analyzed through simple averages, correlation analysis and paired t-test. Average yield of bitter -1 gourd crop was 23569 kg acre with cell phone use of 8h per season. It is perceived...

 Hassnain Shah*, Nadeem Akmal*, Waqas Farooq*, Muhammad Azeem Khan* and Shahid Munir**

SHIFT FROM SITE DEVELOPMENT TO SKILL DEVELOPMENT: A CASE STUDY OF WATER HARVESTING THROUGH MICRO CATCHMENTS
...o cost effective, water, labor and resource saving along with farmer friendly under rainfed condition with scare supplemental water availability. The cost of adoption of technology including labor charges for its preparation and maintenance was recovered from irrigation cost saved from single rainfall at the demonstration site. One of the important implications drawn in this regard was the need of a shift from site developme...

 Nusrat Habib*, Saima Rani*, Sabeen Siddiqui*, Shah Zaman** and Muhammad Zubair Anwar*

IMPACT OF MAJOR FARM INPUTS ON PRODUCTIVITY OF SUGARCANE: A CASE STUDY IN TEHSIL KOT ADDU, PUNJAB, PAKISTAN
...rrigation, FYM, seed and labor for harvesting were the significant factors which affect on the proceeds of sugarcane growers. The results revealed that costs of land preparation, DAP and urea were highly significant at 1% level with positive coefficients 0.94, 0.23 and 0.76, respectively. The cost of FYM was significant at 1% level with negative impact on sugarcane productivity (-0.07). The coefficient 2 of multiple determinations R was 0.72, which designated ...

 Nosheen Zahra*, Ghulam Sarwar* and Sher Muhammad**

COMPARISON OF GYPSUM AND POTASSIUM SILICATE FOR RECLAMATION OF SALINE SODIC SOIL
...ncy was assessed through laboratory analysis. After this, rice seedling (Shaheen Basmati) was transplanted in all the pots i.e., 3 plants per pot. Necessary N, P and K fertilizers were applied at the recommended rate. After crop harvest, soil samples were taken for analysis of pH, electrical conductivity (EC), sodium adsorption ratio (SAR) and exchangeable sodium percentage (ESP) of soil. All the amendments when used alone or in combination with each other imp...

 Muhammad Aamir Khan*, Abdul Saboor*, Rauf-i-Azam**, Abdul Qayyum Mohsin* and Fayyaz-ul-Hassan***

IMPACT OF AGRICULTURAL TRADE LIBERALIZATION ON INCOME INEQUALITY IN PAKISTAN
... multiple households and labor types. This disaggregation allows to analyze distributional impacts and to implement policy scenarios that target particular households. The economy wise results showed that agricultural trade liberalization increases income inequality and somewhat detrimental to the rural household types, who guage most of their income from agricultural business. Trade policy must be pursued keeping derivation and distributional narration in vie...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...brionidae) larvae in the laboratory. The objective was to examine changes in production or activities of carboxylesterase (CE), total esterases (TE), alpha-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri dishes. The surviving ones were then homogenized in ...

 Amjad Pervez*, Syed Muzaffar Ahmed*, Akhlaq Ahmad** and Qazi Mehmood Ali**

EVALUATION OF DIFFERENT FOOD GRAINS AS BAITS FOR MANAGEMENT OF HYSTRIX INDICA

 Khurram Nawaz Saddozai*, Umme Rubab* and Abass Ullah Jan* 

STOCHASTIC FRONTIER ANALYSIS OF MAIZE FARMERS IN AZAD JAMMU AND KASHMIR, PAKISTAN
..., tractor hours, FYM and labor days have contributed significantly to increase the maize yield. However, the DAP and urea have shown no effect on maize yield. The mean technical efficiency was estimated at 83%, implying that the farmers can still enhance their technical efficiency by 11% within the given inputs and technology. The results have demonstrated that maize crop is lucrative crop in the study area as maize growers have received increasing return to s...

 Ambreen Akhtar Saddozai*, Amer Mumtaz*, Saeeda Raza* and Shahzada Arshad Saleem** 

MICROBIAL COUNT AND SHELF LIFE OF PHALSA (GREWIA ASIATICA) JUICE
...igated the shelf life of laboratory developed phalsa juice at room temperature. Phalsa was purchased from local market, juice was prepared and kept in sterilized bottles at room temperature. Physicochemical and microbial and oragnoleptic quality of the juice was examined till two weeks. Microbial activity in phalsa juice increased while organoleptic attributes such as texture (mouth feel), flavour, taste, colour and overall acceptability of phalsa juice were d...

 Muhammad Nisar Khan*, Tariq Hassan*, Hassnain Shah*, Saleem Abid*, Irum Raza* and Saqib Shakeel Abbasi*

ASSESSMENT OF THE PROFESSIONAL TRAINING COURSE UNDER WATERSHED PROJECT AT FATEHJANG FIELD STATION, PUNJAB, PAKISTAN
...uo; in April, 2014 in collaboration with ICARDA and USDA. Twenty seven professionals from different organizations and departments namely; AlMustafa Development Network (ADN), Taleem Foundation, Islamic International University (IIUI) Islamabad, National Agricultural Research Centre (NARC), National Centre for Rural Development (NCRD), National Rural Support Programme (NRSP), On-Farm Water Management (OFWM), Water Management (WM), Potohar Organization for Devel...

 Muhammad Qasim*, Khuda Bakhsh**, Sultan Ali Tariq*, Mahmood Nasir***, Rashed Saeed**** and Muhammad Ather Mahmood****

FACTORS AFFECTING GROUNDNUT YIELD IN POTHWAR REGION OF PUNJAB, PAKISTAN
...frequency, seed rate and labor mandays have positive relationship with groundnut productivity. Therefore, the provision of improved groundnut production technologies package and improved seed to groundnut growers may enhance the productivity and area under this crop.

...

Sumia Bint Zaman*, Waqas Farooq*, Sidra Majeed*, Hasnain Shah*, and Abdul Majid**

ASSESSMENT OF AGRICULTURE SERVICE PROVIDERS' TRAINING ON WATER CONSERVATION TECHNOLOGIES IN POTHWAR REGION
... being implemented in collaboration with ICARDA and other NARS partners. The prime objective of these training programmes was to impart training based skills on water conservation technologies. To help in improving the effectiveness, the socioeconomic backup for assessment of these trainings was included as part of the activity. The information for paper is collected from opinions of the participants before and after the training. Descriptive analysis alongwit...

 Farid Asif Shaheen*, Sadia Parveen*, Ahmed Zia**, Ghulam Qadir*,
Mureed Husain*** and Rifat Ullah Khan****

PREDATORY APTNESS OF ANTS AGAINST RED FLOUR BEETLE, TRIBOLIUM CASTANEUM HERBST (TENEBRIONIDAE: COLEOPTERA) IN WHEAT FLOUR
...ulture was maintained in laboratory. Three ant species namely, Dorylus labiatus, Camponotus rufipes and Monomorium minimum were collected from forest and non-forest habitats and compared for their predation against different life stages of RFB. Results showed D. labiatus of forest habitat as an efficient pupal predator that consumed 91.66% pupae of RFB. It was significantly different from non-forest ant population and control with 73.33% and 11.66% pupal preda...

 Inayat-Ur-Rahman*, Shamsher Ali**, Mukhtar Alam*, Mohammad Adnan*, Hidayat ullah*, Muhammad Faisal Anwar Malik*, Abdul Sattar Shah* Mohammad Ibrahim*

EFFECT OF SEED PRIMING ON GERMINATION PERFORMANCE AND YIELD OF OKRA ( L.)
..., two experiments, i.e., laboratory and field were carried out during 2013. In the laboratory experiment, okra seeds were primed in PEG-8000 (polyethylene glycol) and Mannitol solutions (0, -0.4, -0.8, -1.2, -1.6, -2 and -2.4 Mpa osmotic potential) for 6, 12, 18 and 24 hours while dry seeds were used as control. Same primed seeds were used in the field experiment. Priming proved effective in improving percent germination and...

 Muhammad Bilal*, Zafar Abbas*, Ijaz Ahmad**, Muhammad Aslam*, Muhammad Ijaz****, Adnan Shakeel***, Mushtaq Ahmad*** and Kashif Bashir*****

RESPONSE OF DIFFERENT COTTON ( L.) GENOTYPES AT VARIOUS SALINITY LEVELS
...in soil at water testing laboratory, D.G. Khan during 2012-13. Six cotton genotypes (FH – 945, FH – 901, NIAB – 111, NIAB – 999, B – 630 and B – 622) were collected from Nuclear Institute for Agriculture and Biology (NIAB) and Ayub Agricultural Research Institute (AARI), Faisalabad. The plant height of NIAB-111 was more affected by soil salinity than that of other varieties. The number of bolls per plant also showed the simi...

 Arshia Naeem*, Maria Anjum*, Mariam Rehman*, Zahid Mahmood** and Muhammad Asif Kamran**

AN INTEGRATED INFORMATION SYSTEM TO FACILITATE FARMERS IN WHEAT, SUGARCANE AND OTHER CROP DISEASES IDENTIFICATION
...form was evaluated in collaboration with researchers from Ayub Agricultural Research Institute (AARI), Faisalabad by employing walkthrough and survey with farmers and agriculturists. The study is first attempt in terms of practical usage of ICT tools to bridge research and extension gap and can be used by agricultural policy makers to support agricultural services for farming communities who are otherwise deprived of extension services due to large number of f...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
...R50, respectively. Under laboratory conditions, the mortality percentages were 83.24, 85.13, 94.21 and 97.36% for A. lycopersici and 17.14, 27.55, 37.14 and 59.37% for N. cucumeris at 1000, 2000, 3000 and 4000 ppm of Huwa-San TR50, respectively. In addition, Huwa-San TR50 caused a concentration dependent reduction in the percentage of egg hatching. The percentages of larvae hatching from eggs of A. lycopersici were 73.42, 56.92, 25.41 and ...

Amjad Ali1, Rajan Shrestha2, Ghaffar Ali1, Irfan Ullah1 and Salman Khan1 

...Results illustrate that labor days, seed quantity, tractor hours, urea and pesticides have positive significant effect on sugarcane yield. Resource use efficiency analysis shows that marginal value product (MVP) to marginal factor cost (MFC) ratios for labor days, irrigation, urea, FYM and pesticides are more than unity indicate underutilization of these resources which underlines scope of expansion in these resources. Stud...

Amjad Ali1, Rajan Shrestha2, Ghaffar Ali1, Irfan Ullah1 and Salman Khan1 

...Results illustrate that labor days, seed quantity, tractor hours, urea and pesticides have positive significant effect on sugarcane yield. Resource use efficiency analysis shows that marginal value product (MVP) to marginal factor cost (MFC) ratios for labor days, irrigation, urea, FYM and pesticides are more than unity indicate underutilization of these resources which underlines scope of expansion in these resources. Stud...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
María Magdalena Crosby-Galván1, Bernardino Espinoza-Velasco1 and Mónica Ramirez-Mella2,*
...dard diet for ruminants elaborated with corn stover, corn grain and soybean meal, and three experimental diets with 10, 20 and 30% dry residue of chihua pumpkin instead of corn stover. Dry matter digestibility and gas production was determined at 24 h of incubation in ruminal fluid obtained from two Holstein cows with a ruminal cannula. Randomized block experimental design was used as and a comparison of means was performed by the Tukey test. The results indic...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...techniques and different laboratories are practicing variable quality of screening blood screening. All diagnostic techniques must be regulated under a standard quality control and a nation wise validation study is highly recommended.
...

Nihal Ahmad Hadi, Shahid Ali* and Umer Wahid

 

... checks, feed intake and labor have positive and significant effect on broiler output. Technical inefficiency results showed that estimated coefficients of age of broiler farmers, education and experience were negative and statistically significant. This means that with the increase in age of broiler farmers, education and experience, technical inefficiency decreases. It is recommended that broiler farmers needs to increase the number of day old chicks accordi...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
...n the present study, one laboratory reference strain and five field strains each of, Rhyzopertha dominica, Sitophilus oryzae and Tribolium castaneum, were collected from Punjab, and assessed for their susceptibility to spinosad via diet incorporation bioassays. Based on median lethal concentration (LC50) values, all the strains of S. oryzae and R. dominica were more susceptible to spinosad than T. castaneum ...
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
...ined individually in the laboratory. Both the control and insecticide treated spiders were offered larvae of Drosophila melanogaster in different densities. C. citricola increased prey consumption as the prey density was increased. Insecticide treated C. citricola consumed less prey as compared to control. Effects of chlorpyrifos on the prey consumption of C. citricola were more drastic than bifenthrin. It was also recorded that at ...
Arshad Mahmood Malik1, Khalid Mahmood Mughal2, Sarfraz Ahmed Mian2 and Atta Ullah Khan2
... with and without casual labor respectively. Analysis suggests that this technology can improve the yield of tomato in the country and solve the demand and supply gap of Pakistan besides helping in export promotion and import substitution. Various factors identified in analysis have been further examined and measures have been suggested to improve supply of tomato in Pakistan. 

...
Gulnaz Malik1, Abdul Qadir1 and Hafiz Azhar Ali Khan2,*
...h S. oryzae under laboratory conditions. The toxicities of spinosad, emamectin benzoate, lufenuron and thiamethoxam increased 2.65, 1.59, 1.64 and 3.00 folds (positive temperature coefficient), respectively, with increasing temperature. The positive temperature coefficients of all the tested insecticides suggest that these insecticides may provide effective control of rice weevils under high temperature conditions.

 

...
Ayesha Iftikhar1,*, Muhammad Asif Aziz1, Muhammad Naeem1, Munir Ahmad1 and Tariq Mukhtar2
... sexmaculatus in the laboratory and use as a bio-control agent against cotton mealy bug in the field at different temperature conditions.
...
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
...TB Research Centre in collaboration with Department of Pulmonology, King Edward Medical University, Mayo Hospital, Lahore.
Methods: Sputum samples from 125 patients were collected from confirmed pulmonary TB patients who were not responding to standard regimen of first line anti-tubercular treatment. Smears were stained by Ziehl-Neelsen method. All specimens were processed for culture and drug sensitivity by drug proportion method using Lowe...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
Muhammad Sarfraz*, Shahida Hasnain
...and Cefepime. The study elaborates recent trends in microbial profiles of pAmpC beta-lactamase producing pathogens in Lahore, Pakistan. This knowledge will enable the medical laboratories to report pAmpC β-lactamase detection accurately and support physicians to prescribe the appropriate antibiotics.
...

Dilshad Ahmad1*, Muhammad Irfan Chani1 and Muhammad Afzal2 

...al credit, cropped area, labor force participating in the agriculture sector, trade openness has been quantified through various econometric approaches in the study. Autoregressive Distributed Lag (ARDL) Bound testing approach has been applied to analyze the long run cointegration among variables, obtaining annual time series data from 1973 to 2014. An empirical estimation of ARDL approach has indicated the evidence of long-run cointegration of model. Empirica...

Ansaar Ahmed1, Imtiaz Hussain2*, Ibni Amin Khalil3, Subhanulla3, Gulzar Ahmed3, Imtiaz Ahmad3 and Muhammad Imtiaz2  

...ce that requires skilled labor, intensive tillage and more water for irrigation. In this study, zero tillage and bed planting effect in comparison with farmer practice on maize-wheat system productivity was evaluated at five sites in district of Nowshera, KP province during 2014-2016. Maize grain yield with bed planting and manual planting on flat surface were non-significantly different. Because of weed infestation, there was 30 percent lower maize grain yiel...

Sadiqa Begum1, Murad Khan2* and Noor Pao Khan

...luding seed, irrigation, labor, fertilizer, pesticides and tractor used in production of wheat were having positive relationship with the production. District Peshawar is more efficient in production of wheat which can be raised further by proper utilization of all the inputs. 

...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
... were investigated under laboratory condition. The highest mortality of S. litura was observed at early instars. Against second instars of S. litura, LC50 values of NPV isolate ranged from 1.92×103 to 3.64×103OB/ml with LT50 values of 69.30 h to 72.80 h, respectively. This study showed highly effectiveness and provides an opportunity to cut down the use of synthetic approaches and develop sa...
Xiaqing Chen, Yü Huang and Zhen Huang*
...gicide carbendazim under laboratory conditions. The results showed that S. shiraiana was not detected on mulberry leaves and branches, whereas it was only detected in mulberry fruits. T. hamatum fermentation broth and its ethyl acetate extracts caused high mycelial growth inhibition and germination inhibition of S. shiraiana, with the inhibition value up to 80% and 90%, respectively. An apparent increase in mycelial growth inhibitio...
Haroon Rashid1,*, Khushi Muhammad1, Masood Rabbani1, Muhammad Sarwar Khan1, Tasleem Ghori2, Mamoona Chaudhry3, Hamad Bin Rashid2, Muhammad Zubair Shabbir1, Muhammad Hamid Chaudhary4, Muhammad Asad Ali1, Mian Muhammad Khubaib Sattar5, Javed Muhammad1 and Bhushan Jayarao6
...v>A multidisciplinary collaborative project was conducted to study the prevalence and distribution of soil-borne B. anthracis in Punjab Province, Pakistan and to ascertain its public health significance. Using a grid-based sampling strategy, soil samples (n=1985) were collected from villages (n = 397, 5 samples/village) and examined for Bacillus anthracis, using real time PCR assays. Soil samples were found positive for plasmid DNA of Bacillus...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...C) are used by different laboratories. In this study, the storage stability of five bacteriophages, JHP, RLP, RSP, SaPL, and IttPL, at distinct temperatures for complete one year is described. Our results indicated that storage of bacteriophages at 4oC was most appropriate, followed by storage at -20oC and -80oC as no drop in viral titer was witnesses in bacteriophage samples stored at 4oC for up to one year. The res...

Amjad Ali1, Abbas Ullah Jan1, Lal Almas2, Noor Piao Khan1 and Khurran Nawaz Saddozai

...nd per acre inputs cost (labor hired, seed, tractor hours, irrigation, Urea, DAP, FYM and pesticides) were considered determinants for cost function. Respondent age, farming experience, education, family size, off-farm income, farm to home distance, tenancy and extension contacts are determinants in allocative inefficiency model. Results of the study revealed that except farmyard manure and pesticides all other explanatory variables are significant influencing...
Saleh S. Alhewairini
...effect on its eggs under laboratory conditions. The mortality percentages of O. afrasiaticus were 61.79, 73.58 and 82.90%; 62.87, 73.77 and 83.32%; 71.70, 82.23 and 92.37% after one week of exposure to the quarter recommended dose (QRD), half recommended dose (HRD) and recommended dose (RD) of oxamyl only and with the recommended dose (RD) of Injo200 or 1000ppm of Huwa-SanTR50, respectively. The percentages of larvae hatching from eggs of O. afrasiat...
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
...nfection among students, laboratory staff and the author throughout the whole period due to contact with these fish. One reason for this is the use of safety measures and precautions by the concerned; such as use of disposable gloves, thorough hand washing, using sterilized equipment, keeping work place clean and disinfecting it regularly. The possibility of isolation of fewer pathogenic agents in this study cannot be ruled out. There has not been any report o...

 Sadia Murawwat1, *Nageen Fatima1, Qandeel Iqbal1, Syeda Ameena Rizvi1 and Mehak Fatima1

ELECTRICAL ENGINEERING BEYOND CLASSROOM: CHALLENGES AND ANTICIPATED RESOLUTIONS
...uch of hardware research laboratories at post graduate level, insufficient practical trainings, internships and professional skill development for improvement of hands on experience. These factors influence an Engineering graduate even after the completion of their undergraduate studies. After sorting these highlighted factors, this work has presented a framework for the improvement in EE as, modified curriculum, e-learning environment, conduction of internshi...

 Muhammad Mohsin Ahsan1, Hafiz Muhammad Tahir2*, Muhammad Khalid Mukhtar1, Arshad Ali3, Zafar Iqbal Kahan4, Kafeel Ahmed4

Intra- and inter-specific foraging in three scorpion species
...istan, was studied under laboratory and field conditions. Results of the study revealed that all three scorpion species were cannibalistic. Mesobuthus tumulus attacked and fed more upon juvenile and sub- adults (medium- sized), scorpions. They also consumed dead scorpions of their own species. Odontobuthus odonturus preferred to attack and consume healthy adults and did not feed on dead scorpions. Androctonus finitimus aggressively attacked and consumed all ty...

 Muhammad Khalil Ahmad Khan1*, Muhammad Zafar2, Munazza Perveen3, Munir Ahmad3, Asia Iqbal4, Asmatullah3

Efficacy of malathion and carbosulfan against crop invadedaphid types Aphis craccivora (Koch) and Aphis gossypii (glover) on bean and brinjal plants
.../> respectively. In the laboratory, the vegetable plant leaves being applied by residual
film technique with tested Aphid population. The most toxic insecticide was found
to be malathion with LC50 as 20.11 μg cm-2 for A. craccivora and 25.28 μg cm-2 for
A. gossypii while carbosulfan was found to be least toxic with LC50 as 312.80 μg
cm-2 for A. craccivora and 322.25 μg cm-2 for A. gossypii respectively. No
signif...

 Zafar Iqbal

A review of fisheries education in University of the Punjab, Lahore, Pakistan
...se and health Management laboratory (FDHM) was
established in 2004 and it is providing research facilities in Fish Parasitology, Fish
Mycology and Fish Bacteriology to researchers. Over 60 students have completed
their research projects in fish disease and health management. The future aspects
of “Fisheries Education” needed in aquaculture industry in Pakistan and Asia
Pacific Region is discussed.

...
Muhammad S. Waqas, Ali A.Z. Shoaib, Xinlai Cheng, Qianqian Zhang, Asem S.S. Elabasy and Zu-hua Shi*
...e investigated under the laboratory condition. Our results demonstrated that P. solenopsis reproduce sexually. Males mated 1-2 times in one day and 3-6 times in their lifetime, and did not show mating preference for female age or density. Although a few unmated females produced ovisacs, while they neither produced eggs nor gave birth any crawlers in the ovisacs. Unmated females lived for longer durations than the mated ones. Sexual reproduction with sho...
Ajmal Khan* and Rehan Ullah
...ents reported to various laboratories of Agency Head Quarter Hospital Khar, Bajaur Agency. In this study, a total number of 9720 suspected individuals were examined; among which 1273 (13.1%) were found to be positive for malaria infection with different Plasmodium species in their blood smears. Male population accounts for higher prevalence rate (14.59%) than female (11.24%). The infection was more prevalent in November (21.4%), December (21.1%) and Oct...
Muhammad Asad Saleem1,*, Mirza Abdul Qayyum1, Mudssar Ali1, Muhammad Amin2, Muhammad Tayyab1,3 and Sumaira Maqsood4
...gy and development under laboratory conditions and survival against sub-lethal doses of B. bassiana and biorational insecticide (Nitenpyram). Sugarcane sets as alternate to date palm stem were successfully used as diet for rearing RPW. Combination of entomopathogenic fungus (Bb) and Nitenpyram (Nit) were found more lethal to survival of RPW. A reduction in pupation and adult emergence was recorded in the combined treatments. Moreover, decrease in weight...
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
...d no choice method under laboratory controlled conditions. In M. oleifera extract, the maximum antifeedant index was recorded in 125.00 mg/ml (64.93±2.61), while minimum antifeedant index was observed in 7.81 mg/ml i.e. 8.39 ± 1.85 by no choice method. In case of M. paniculata extract, the maximum antifeedant index was 98.90±0.67 in 125.00 mg/ml, while minimum antifeedant index was 30.90±1.85 in 7.81 mg/ml by no...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
... Sargodha), under laboratory conditions. Both NPV and Bt were applied using diet incorporation method. Two concentrations of a NPV based formulation (Somestar): 1x107 and 1x108 POB/ml, and three concentrations of a Bt based formulation (Dipel): viz., 0.1, 0.3 and 0.5 μg/g, were applied alone and in different combinations against 2nd and 4th instar larvae of S. litura. The highest...

Syed Amjad Kamal Jan* and Naushad Khan 

...ng experience, Number of labors were found non-significant at 5% level. The R-Square value was 0.92 which means that 92% variation is explained by the independent variables on the dependent variable in the model. It seems very good fitted model as R-Square is much high for it. The F-statistic value was found 113.97 which shows that model overall is significance. Few problems and constraints were observed such as lack of credit facilities; Lack of agriculture e...
Huseyin Cetin1,*, Oner Kocak2, Emre Oz1, Samed Koc1, Yesim Polat1 and Kalender Arıkan2
...ntep and Şanlıurfa) in laboratory conditions. According to results the use of PBO in combination with insecticide active substances causes an increase in the mortality rates after 24 h. Also the toxicities of tested chemicals, based on their KDT50 (Knock-down 50 times) values, increased when mixed with PBO.
...
Mehwish Akram1,2,* and Naeem Rashid1
...r studies are needed to elaborate the physiological roles of Drosophila potassium channels which may lead to a better understanding of human Kir channels related to pathological conditions and diseases.
...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
Liyan Zhang1,2, Zhidong Zhou1,3, Haiping Li1,2, Yanlong Qiao4, 5 and Yueping Zhang1,3,*
...population genetics and elaborate the historical demography of Scomber japonicus collected from four geographical locations in the Taiwan Strait and its adjacent waters. The results of genetic diversity and genetic structure analysis of S. japonicus showed that these four populations exhibited moderate genetic diversity across the sampled geographical range of the species. However, no phylogenetic structure corresponding to geographic location wa...
Temel Göktürk1,* and Göksel Tozlu2
...pact was monitored under laboratory conditions. The finding of the study revealed that the most effective dosages for larvae of D. pini was Dipel 300 g/100 l and Dipel 500 g/100 l of B. thuringiensis. The efficiency ratio of Pyrethrum doses ranged from 59.5%-78.5% for Spunizet Neu and 85.5%-95.5% for Dipel DF against D. pini larvae. It was observed that the dosage of both pesticides was directly propositional to the death in larvae...

Dilshad Ahmad1* and Muhammad Afzal2

...ed a cropped area, seed, labor, fertilizer and irrigation were the significant variable of increasing cotton production of Bt farmers while pesticides, irrigation and extension services more significant in increasing the Non-Bt cotton farmers production. Age of farmers, educational status, credit access and agriculture extension services access have significantly and positively impacted while the shortage of irrigation negatively impacts in both districts Non-...

Aqsa Khan1, Sabyan Faris Honey2*, Babar Bajwa2, Nelofer Jamil1 and Muhammad Sohail Mazhar2

...rear codling moth in the laboratory for different research purposes, three artificial diets with different compositions were evaluated for codling moth larvae. Two diets containing wheat germ in their composition as major protein source and one diet without wheat germ were used. Addition of wheat germ in the diet composition for larvae resulted in significantly high percent survival (maximum value) (60± 4.56 and 56± 2.56) and significantly increa...

Shafique Ahmed Memon1,6, Arif Ali1*, Mehar-un-Nisa Narejo2, Ghulam Khaliq3, Imran Ali Rajput4, Muhammad Adeel1, Khalil Ahmed Memon5, Dzolkhifli Omar6, Muhammad Rashid7 and Abdul Rasool

...ra cephalonica under the laboratory conditions (25±2 °C). The results indicated that a significantly increased pre-imaginal (19.93±0.24) and mean average days (70.33±0.55) of C. nipponensis on R. Maidis and also a significantly increased pre-imaginal (20.84±0.49), (21.22±0.97) and mean average days (74.18±0.33), (68.72±0.30) of P. ramburi and Apertochrysa sp. on the host of R. maidis. Whereas a significantly...
Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...us leafminer larvae. Two laboratory bioassays, leaf dip bioassay (LDB) and topical bioassay (TB) were developed to check the efficacy of tested chemicals and essential oils. As seen from results, abamectin showed significant mortality (63.5%) of CLM larvae when topical bioassay was performed. Similarly, the percent mortality of CLM larvae was 53.8% after application of abamectin, when leaf dip bioassay technique was used. However, among tested botanicals, A...

Bata Shalangwa Ishaku1, Batim Turdam1, Maimadu Abdullahi1, Waziri Ibrahim Anjili2 and Mayowa Olabode2 

...nalyzed using a standard laboratory procedure. Coprological examination conducted using flotation and sedimentation techniques showed that 276 (92.0%) were positive for gastrointestinal nematodes. A greater proportion (70.7%) of the nematodes were Strongyles followed by mixed infection of Strongyles and Parasacaris equorum (15.2%) and Strongyloides westeri (14 1%). Chi-square test and odds ratio were used to analyzed data obtained. The prevalence of the gastro...
Ahmed Ali Samejo* and Riffat Sultana*
...t in field as well as in laboratory condition. Comparative morphometry revealed that in relation to previous stages 2nd and 3rd nymphal stages gained much more length and weight as compared to 4th and 5th nymphal stages. Correlations between length and weight of hoppers of each nymphal stage were positive. It could be concluded that 4th and 5thinstars were voracious in consuming vegetation and h...

Syed Atif Hasan Naqvi* 

...gement. Evidence from my laboratory suggest that biological control is the best option having ecofriendly activities for the management either using botanicals or through some biological agents. Formulations of various botanicals along with biological agents may be commercializing to help farmers to combat the disease. There is a need to use environmentally safe approaches to overcome the loss of grain yield in rice due to this disease. Climate change has a se...

Arshad Farooq* and Muhammad Zafarullah Khan 

...us factors such as land, labor, finance and management practices etc. This article assessed the knowledge gap of improved cane management practices in Khyber Pakhtunkhwa-Pakistan. Sugarcane growers were selected randomly and Data were collected through interview schedule from 285 respondents in D.I. Khan and Mardan districts during sugarcane cropping season, 2018. Knowledge gap index was applied as a measuring tool. The empirical results indicated th...

Shahid Ali1*, Asim Khan1, Aftab Khan1 and Bakhtawar Riaz

...% increase in seed rate, labor, tractor hours, urea, DAP and irrigation increases output by 0.81, 0.02, 0.28, 0.14, 0.25 and 0.02 percent, respectively. Mean technical efficiency score was found 95% ranges from 86% to 99%. This means an average farmer can increase tomato output by 4% with available resources and technology. Results of inefficiency effect model indicated that farmers’ experience has negative and significant relationship with technical ine...

Bina Khanzada1*, Ghulam Hussain Abro1, Tajwar Sultana Syed1 and Nazir Ahmed2 

... of the parasitoid under laboratory conditions and compared with the provision of flower nectors such as ornamental sunflower, merry gold and hollyhock in the laboratory. The ornamental plants sunflower, merry gold and hollyhock were also tested in the field for conservation of the C. flavipes. The results showed that during 2013 and 2014, the C. flavipes fed on Hollyhock produced more cacoons. During 2013 and 2014 C. flavip...

Essossinam Ali* 

...tolerant seeds (DTS) and labor allocation. Multi-stage sampling technique was used to collect data from 704 farm households in three regions (Central, Kara and Savannah) in subsistence agriculture in Northern Togo. Tobit and linear regression models were used to analyze the effect of farmers’ risk aversion on fertilizer and DTS uses, and labour allocation, respectively. The results indicate that on average 86.22% of farmers have risk aversion behaviors. ...

Mehran Ahmad, Syed Attaullah Shah and Shahid Ali* 

.... DAP, Nitrate and Urea, labor and FYM had positive significant effects on tomato yield. Estimated allocative efficiencies of DAP, Nitrate and FYM, were greater than 1, indicating that growers were underutilizing these inputs. This might be due to growers’ lack of knowledge or budget constraint. Nitrophous and chemicals were over utilized as their allocative efficiencies were less than 1. The study recommends that government may provide subsidized inputs...

Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...ational pesticides, this laboratory study was carried out to evaluate few selected novel chemistry insecticidal formulations against 2nd instar nymphs of D. mangiferae using standard twig-dip bioassay method according to Completely Randomized Design. Results showed that both factors i.e. insecticidal treatments (F 9, 245 = 146.90, P < 0.001) and time (F 4, 245 = 445.75, P < 0.01) and their interaction (F 36, 245 = 9.20, P < 0.001) had a significant ef...
Rabia Khalid1, Saleema Bashir Shams1, Bibi Nazia Murtaza1,*, Gaitee Joshua1, Saira Mushtaq1, Hassan Al-Talhi2 and Abdulaziz Al-Amri2
...ed. The experimental and laboratory values for BMI, total cholesterol (TC), total triglycerides (TG), high density lipoproteins (HDL), low density lipoproteins (LDL) and fasting blood glucose (FBG) were used to demonstrate the incidence of metabolic syndrome. Overall, 39.025% of bank employees and 22.53% of randomly selected control individuals have metabolic syndrome. Among the bank employees, prevalence of obesity was 67.53% and in general population it was ...

Amany Adel, Wesam Mady*, Zienab Mosaad, Fatma Amer, Asmaa Shaaban, Dalia Said, Marwa Ali, Abdel-Satar Arafa, Mohamed Kamal Morsi and Mohamed Khalifa Hassan  

...bmitted to the Reference laboratory of veterinary quality control on poultry production (RLQP) from different poultry sectors and bird species which are distributing all over the Egyptian governorates for examination of LPAI (H9N2) virus by real time RT-PCR. The results confirmed positive samples from 1026 for H9N2 with prevalence rate 4.4%. However, the LPAI H9N2 showed a wide range distribution with high geo-prevalence rate in 2015/2016 (96.3%) as positive s...

Kamran Aziz1*, Aamir Saleem1 and Arshad Mahmood Malik2 

...chemical analysis in the laboratory and litter fall production was estimated by trapper technique. Under Cedrus deodara and Pinus wallichiana canopy, the leaf litter was collected and brought back to laboratory for the further chemical analysis. The Total annual litter fall production of Cedrus deodara was 4.2209t/ha/annum while the Pinus wallichiana was estimated 4.6097t/ha/annum. It showed that there is more litter fall in...

Arshad Mahmood Malik1*, Khalid Mahmood Mughal2 and Abdul Saboor3 

.... Results indicated that labor used in production of hydroponics products and supply chain management of hydroponics products both in the domestic market and in regional markets has significantly affecting total value product of the hydroponics. Return to scale value of 1.77 indicated that the product function is positive and increasing return to scale in TVP in relation to increase in input cost. The value of (DRC<1) indicated that Pakistan has comparative...

Zafar Abbas1*, Muhammad Mubashir1, Umair Riaz1, Zeenat Javid1, Muhammad Ashraf1, Saeed ur Rehman1, Muhammad Javid Qamar1, Syed Ali Zulqadar1 and Shahzada Munawar Mehdi2 

...f soil and water testing laboratory for research, Bahawalpur in growing seasons from July to October of 2017. Treatments were arranged in Two factorial Completely Randomized Design CRBD fashion with three replications and six treatments. Treatments are arranged as control T1 (Without fertilizer + No Poultry Manure + No compost), T2 (NPK full dose @ 150 kg ha-1N, 75 kg ha-1P and 60 kg ha-1K), T3 (full dose of poultry manure (PM) @ 30 ton ha-1), T4 (50% NPK+50% ...
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2
...iagnostic centre’s/laboratories of Pakistan for ELISA and PCR tests. Out of 703 dengue positive samples, 93.5% were anti-dengue IgM positive, 71.5% were anti-dengue IgG positive and 81.3% were anti-dengue NS1 positive. Among these seropositive samples, 39.66%, 40.15% and 36.88% were PCR positives, respectively. This immunological and genomic study reveals that there were more seropositive patients than RNA positive ones. Serotype-2 remained the main caus...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Group IV: M199 alone, Group V: M199 + co-culture and Group V...
Ghaleb Adwan*, Sami Bdir and Kamel Adwan
...studies are needed using laboratory experimental infections to clarify the complete life cycle for these larval trematode species.
...
Asad Abdullah, Muhammad Irfan Ullah*, Abu Bakar Muhammad Raza, Muhammad Arshad and Muhammad Afzal
...to artificial diet under laboratory conditions (32±05 oC; 65±05 RH). Thelarval suitability was found maximum on cabbage followed by sesbania, alfalfa and minimum on maize. The larval length and weight were found higher on cabbage followed by alfalfa and sesbania. Similarly, the pupal development was minimum in respect to days on maize than others. The adults lived 11 days more on cabbage compared to other diets. Female life span was fo...

Muhammad Ishaque Mastoi1*, Ibtasam Riaz2, Syed Muneeb Haider2, Arfan Ahmed Gilal3, Anjum Shehzad4, Ahmed Zia4 and Abdul Rauf Bhatti4
 

...n regulation. Thus, this laboratory study was conducted to evaluate feeding potential of two coccinellid predators i.e., Menochilous sexmaculata and Coccinella septempunctata on eggs and nymphs of P. minor. Results confirmed that both species of coccinellid showed feeding potential against eggs and nymphs of P. minor. Comparatively, M. sexmaculata showed higher feeding than C. septempunctata on various immature stages of M. minor. Moreover, both the species sh...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
Saleh S. Alhewairini
... RPW was evaluated under laboratory conditions. RPW (larvae and adult) showed high susceptibility to both acetamiprid and sulfoxaflor. In addition, both insecticides had significant effect on the mortality of RPW (larvae and adult) except the pupal stage under laboratory conditions. After direct spray application of the recommended dose, the adult mortality percentages were 66.67, 73.33 and 100% for acetamiprid and 23.33, 60...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
Muhammad Aslam Farooqi*, Bakhtawar Irsa, Sajjad Ali, Asif Sajjad, Muhammad Waqar Hassan and Sohail Akhtar

 

...tion was performed under laboratory conditions (28±2°C and 65±5% R.H). Four different concentrations (i.e. 50 ppm, 100, 200 and 400 ppm) of each insecticide were evaluated against A. mellifera as derived from their recommended field doses. Mortality of A. mellifera was recorded at 0.5, 3, 6 and 24 hours after exposure to insecticides. To evaluate the toxic impact, median lethal concentration (LC50) of each test...
Po Bunnika, Khalid Khan and Guo Xiangyu*
...ctive use of capital and labor by increasing agricultural output.
...
Sadreddin Tusun*
...ey and maintained in the laboratory. The different parts of the third instar larvae such as antennae, head, mandibles, abdomen and types of sensory receptors are described and illustrated.
...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...arvae were reared in the laboratory. When  samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collected afterward. It is concluded on the basis of results that EPNs is positively recorded in grassy land, tomato field, forest land an...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...ment contamination. This laboratory study evaluated the toxicity of methanolic extracts of eight flowers i.e. Mexican marigold (Tagetes lucida), African marigold (Tegates erecta), tecoma (Tecoma stans), calendula (Calendula officinalis), basil (Ocimum basilicum), oxeye daisy (Leucanthemum vulgare), lily (Lilium longiflorum) and chrysanthemum (Glebionis segetum) against the worker individuals of subterranean termite Odontotermes obesus (Isoptera: Termitidae). B...

Wajid Shah Yousafzai1, Irfan Ullah1*, Amjad Ali1, Mahmood Iqbal2 and Salman Khan1 

...ive efficiency value for labor days was 1.07suggests almost near to allocative efficient level. The study recommends season long trainings for optimal resource allocation, availability of inputs at reasonable prices and facilitation of growers in marketing of output, in order to enhance allocative efficiency of onion growers in the study area. 

...

Saifullah* and Ihsan Mabood Qazi 

...grains were milled using laboratory scale flour mill. Part of the flour of each variety was passed from mesh to remove the bran and get fine flour. Both whole and fine flour of the selected wheat varieties were subjected to qualitative study. The data disclosed that L* value was more in FWF than WWF. FWF had high Water absorption index (WAI) 9.19-9.25g/g, Swelling Index (SI) 6.6-8.7g/g, Water Solubility Index (WSI) 5.7-9.77% and gluten (20.76-34.45%) content w...

Shahid Ali1*, Farhan1, Murtaza1, Neelum Andaleeb2 and Amjad Ali1 

...ticides and weedicides), labor and tractor hours were statistically significant with the coefficients of -0.027, 0.090, 0.060, 0.223 and 0.056, respectively. The estimated results of SFP model-2 revealed that household’s education level was having negative and significant effect on technical inefficiency. Among the sampled respondents owners were technically more inefficient as compared to the tenants. Farm size was having negative and significant effect...
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslamand  Samina Jam Nazeer Ahmad1, 2
...vel of LC50 values among laboratory reared and field collected population against Cypermethrin. The laboratory reared resistant population, Sindh and South Punjab collected population showed high LC50 values as compared to susceptible, Baluchistan and KPK population. The expression of P450 gene through PCR showed enhanced level of P450 gene in different Instars as well as collected population. The dsRNA specific for CYP450 g...
Ammara Blouch1, Ata ul Mohsin2, Muhammad Naeem2 and Rashid Mahmood1*
.../i>. litura under laboratory conditions using leaf dip method. Mortality was recorded after three and seven days of exposure. The results indicated that larval mortality increased with time and Turex (Bt sub speciess kurstaki and aizawai)after 3 days of exposure caused significantly higher mortality i.e 46.43, 43.45 and 38.69 % as compared to Dipel (Bt sub speciess kurstaki) that caused 19.05, 6.55 and 4.76 % mortality...
Mubasher Ahmad Malik1, Samina Jam Nazeer Ahmad2, Muhammad Jalal Arif1 and Jam Nazeer Ahmad1,*
...>P. xylostella under laboratory conditions. Both AZA and PxGV were applied alone and in integrated manners at LC20 and LC50 dose rates. The combination of 349.3 parts per million of AZA and 4.8×104 occlusion bodies per millimeter of PxGV, resulted in synergistic interaction after 3rd, 5th and 8th days post application while the additive effect was observed only after 1 day post-treatme...

Muhammad Asim1*, Asghari Bano2, Sikander Khan Tanveer3 and Muhammad Umair Aslam

...tors. In this study, we elaborated the role of these factors, in rainfed agro-ecology, using data of six genotypes, grown in four sowing windows over six years. The rainfed wheat productivity is predominantly determined by environmental conditions, particularly rainfall induced moisture availability. Hence, the best match between sowing time and the environment prevailed at that time emerged as an important yield determining factor. Overall, grain yield varied...

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...d brought to the working laboratory for HBV and HCV screening through ICT kit method. All ICT positive samples were further confirmed through ELISA. Individuals 25(8.2%) were found positive for both hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. A...

Anand Kushwaha, Amit Kumar, Aparna Madhavan, Durga Goswami, Golmei Poulinlu and Gnanavel Venkatesan* 

...ize and also to minimize labor/time. However, the genus Capripoxvirus encodes putative 147 proteins in their genome, among which some of them are reported as potential immunogenic candidate genes. Selection and use of such candidate immunogenic proteins from an array of genes located at different structures of a mature virion are the real challenge and time consuming task for researchers. Nevertheless, identification of candidate gene(s) using advanced bioinfo...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2,  Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...e present investigation, laboratory trials were conducted to investigate the synergistic, additive or antagonistic effect of three sub-lethal dose rates (2 x 103, 4.5 x 103, and 6 x 103 PIB/Larva) of native isolated Nucleopolyhydrovirus (NPV) from Spodoptera litura and Spinosad (0.01 ppm) against 3rd and 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A diff...

Haidar Ali1*, Malik Muhammad Shafi1, Himayatullah Khan1 and Hamra Haidar

...e large number of family labors of small farmers were working on high paid jobs or employed else or self-employed and thus contributed a good amount to their family’s income. Firstly, for removing the barriers to the entry of the small farmers into the rural off-farm sector efforts should be made. It demands better educational level in rural areas. Secondly, there should expansion in agricultural wages in rural area. 

...

Stanley Uchenna Onwudike* and Vivian Chizoba Edoziem 

...ried and analyzed in the laboratory. Results showed significant effect (p = 5%) on soil moisture content, soil pH, organic carbon and exchangeable bases between the bulk soil and the rhizosphere. The highest value of saloid bound P (7.77 mg/kg) was found within the bean root zone while the highest value of phosphorus bound to Al (Al - P) (13.27 mg/kg) was found in okra root zone. Highest value of occluded – phosphorus (46.07 mg/kg), Fe -Al–P (40.62...
Chandragouda M. Patil, Shashikant S. Udikeri* and Shreeshail. S. Karabhantanal
...against 651.17 ppm for a laboratory susceptible strain. Thus 27.30 fold resistance ratio was observed (RR) for sulphur. The least resistance ratio 4.45 fold was observed for fenazaquin 10% EC and the field and laboratory susceptible populations have exhibited LC 50 values of 44.62 ppm and 9.57 ppm, respectively. Based on the resistance co-efficient propargite 57% EC, dicofol 18.5% EC and fenazaquin 10% EC have bee...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
... 27±2°C under laboratoryconditions. The results indicated that the larvae of G. mellonella and S. litura were better host as compared to P. gossypiella and L. orbonalis for multiplication of infective juveniles (IJs) of the S. kraussei. Four different concentrations(50, 100, 200 and 500 IJs) of tested EPN speciewere used against insect’s larvae. Reproduction rate of S. kraussei was highest at concentra...
Riffat Sultana1, Nuzhat Soomro1,*, Santosh Kumar2, Ahmed Ali Samejo1 and Samiullah Soomro1
... were examined under laboratory conditions. Significant differences were found in the sizes and shapes of the pods. Beside this, number of eggs in each egg pod, distribution pattern and weight, length and width of eggs in the pods were also noted. Eggs pods of Oxya species were cylindrically elongated, large, slightly bent in the middle and rounded at ends while egg pods of Oxyina bidentata are bean shaped. The weight, width and length of fre...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...> instars larvae of both laboratory-reared and field-collected Aedes aegypti were carried out. The 4th instars larvae of laboratory-reared and the field-collected A. aegypti mosquitoes were exposed to 5000mg/L, 2000 mg/L, 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L of the extract. The solvent for the extract, dimethyl sulphoxide (DMSO) was used as control. The results indicate concentration dependent mort...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...and 22 ppm for field and laboratory population, respectively. Resistance phenomenon is suspected, a rotation of insecticides with different modes of action is desirable in insect resistance management programs.

...

Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq 

...ogoderma granarium under laboratory conditions. The insecticides were tested at four different concentrations (0.25, 0.5, 1 and 2ppm). Mortality of insects was documented after 24, 48 and 72 hours. Abbot’s formula was used to determine the corrected mortality. At 2ppm concentration thiamethoxam and imidacloprid provided 87.57% and 78.59% mean mortality of khapra larvae on treated filter paper after 72 hours respectively. On treated wheat, thiamethoxam pr...

Muhammad Ramzan, Unsar Naeem-Ullah*, Mahreen Hanif, Muhammad Nadeem, Mirza Abdul Qayyum, Muhammad Javaid 

...dy was carried out under laboratory conditions to elicit information about every stage like egg, larva, pupa and adult of Plutella xylostella on natural diet. The study revealed that incubation period of eggs varies from 3.2-4.3 hours. There are four larval instars of Plutella xylostella. The first, second, third and fourth instar larva survived for 3-5,3,1-4 and 2-3 days respectively while pupal and prepupal period lasted for 5-3 days, respectively. Adults li...

Shilong Chen1,2, Shifeng Xiao1,2, Shao Wang1,2, Fengqiang Lin1,2, Xiaoxia Cheng1,2, Xiaoli Zhu1,2, Dandan Jiang1,2, Shaoying Chen1,2* and Fusong Yu3* 

...logy, clinical symptoms, laboratory diagnosis, genomic organization and pathogenesis. However, the pathogenesis mechanisms of cross-species transmission, retardation and beak atrophy are still needed to be elucidated. 

...
Celalettin Gözüaçik1,*, Mustafa Güllü2, Ayda Konuksal3, Reşat Değirmenci3 and Ercan Akerzurumlu3
...covered with nets in the laboratory at 25±1◦C and 65±5% r.h. Following pupation, adult moths and parasitoids were obtained and percentage of parasitized larvae was determined. At the end of the study, Bracon (Habrobracon) stabilis (Wesmael) (Braconidae) and Agathis gracilenta Tobias (Braconidae) species from Hymenoptera order were obtained. Bracon stabilis (93.4%) was determined as widespread and in higher lev...

Aftab Khan1, Shahid Ali1*, Asim Khan1, Muhammad Waqas1 and Sufyan Ullah Khan2 

...aled that tractor hours, labor and FYM positively and significantly affected yield of maize. Average technical efficiency was predicted to be 0.68 ranging from 0.31 to 0.91; implies that an average maize farm can increase yield by 33 percent with existing technology. Technical inefficiency effect model identified that education and farming experience has negative and significant effect on farmers’ inefficiency. It is recommended that maize farmers need t...

Muhammad Farhan1, Ghulam Murtaza1, Muhammad Ramzan2, Muhammad Waqar Sabir3, Muhammad Arsalan Rafique2, Saif Ullah

... investigated in ambient laboratory conditions at University of Agriculture, Faisalabad. Chrysoperla carnea was found very active and consumed all stages of aphid. The predation rate of Chrysoperla carnea was increased with increase in larval instars (1st to 3rd). Third instar of Chrysoperla carnea was very voracious feeder and fed large number of aphid instars nymphs (1st, 2nd and 3rd). The larval predatory potential was 413.9±0.07 aphid per larvae. Th...

Pushpa1, Nighat Seema Soomro1, Shahla Karim Baloch2, Mehmooda Buriro1, Aijaz Ahmed Soomro1, Muhammad Tahir Khan3, Qamar Uddin Jogi1*, Muhammad Nawaz Kandhro1 and Farheen Deeba Soomro1 

...s was investigated under laboratory conditions. The powder of weed plants produced significantly (p<0.05) harmful outcomes on all growth parameters of wheat varieties as compared to the control treatment. The maximum seed germination (82.16a %), shoot length (27.70 cm), root length (14.90 cm), shoot fresh weight (2.19 g), root fresh weight (1.16 g), shoot dry weight (0.54 g), root dry weight (0.27 g), and seed vigor index (3483.5) were recorded in variety A...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1

Amjad Ali1*, Lal Almas2, Syed Attaullah Shah1, Hina Fatima3 and Asim Khan1  

...n inputs side farm size, labor, seed rate, tractor hours, irrigation and urea were found statistically significant. Therefore, based on findings, it is recommended that in order to encourage growers to apply optimal amount of these inputs and avoid uncertainty in returns, an effective system need to be put in place where cost of production might be considered while fixing sugarcane price. Also, there is need to keep experienced farmers involved in sugarcane pr...

Umar Hayat1, Khalid Khan2, Saima Liaqat3 and Guo Xiangyu4* 

...the rice crop (wetland), labor, capital, availability of input, and rice prices are the key components that leave a positive and noteworthy impact on rice production in the District. Nevertheless, physical capital, experience, and age of the farmers have an adverse effect on rice production. Conclusively, it is proposed that the government should introduce precise programs for the upgradation of water systems, farmers’ training, input, and credit accessi...

Mubashir Mehdi1*, Muhammad Bilal Ahsan1, Burhan Ahmad1, Khuram Nawaz Sadozai2, Gulnaaz Hameed3 and Muhammad Asif4 

...or the income of on-farm laborers. In ceteris paribus conditions, coefficient of education indicates that there is a positive relationship among education of labor and Income. Credit facility at farm level was also incorporated as dummy variable under economic context of decent work to assess its regressing level with income and was reported as significant. The study concludes that social upgrading of workers has implication...

Muhammad Usman Saleem, Nadeem Iqbal, Shawaiz Iqbal*, Usama Bin Khalid, Adila Iram, Muhammad Akhter, Tahir Latif and Tahir Hussain Awan 

...pumped groundwater, high labor costs, and drudgery in manually transplanted rice (TPR) have motivated researchers to develop alternated technology such as direct seeded rice (DSR). About 30% of total water used in rice cultivation is consumed for puddling of soil (land preparation) and transplanting operations. Physical properties of soils are deteriorating due to continued puddling over the decades, resulting in structural breakdown leads to a compacted layer...
Fermín López-Uriostegui1, Jesus T. Ponce-Palafox2*, Fabiola Lango-Reynoso1, María R. Castañeda-Chávez1, Itzel Galaviz-Villa1, Sergio Castillo-Vargasmachuca2 and Arturo Ruiz Luna3
...studied under controlled laboratory conditions. Sub-adult’s prawns (10.29 ± 0.27 g) were reared in these conditions for 72 days. Each experimental group contained 25 prawns in an approximately 100-L water capacity tank. The experimental design was factorial with three repetitions per treatment. Prawns were fed (commercial shrimp feed with 35% protein, 12% lipid) daily at a ratio of 10% body weight, twice a day (10:00 and 18:00 h). The optimal grow...

Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy

...lus granarius (L.) under laboratory conditions (25±1°C, 60±5% RH). Diatomaceous earth was applied to wheat grain at the rates of 100, 250, 300, 500, 750, 1000, 1500 and 2000 mg/Kg. Adult mortalities were assessed after 7, 14 and 21 days. Mortality rates rose with increases in dosage and length of exposure. Significantly higher mortality (89.75±3.66%) after one week occurred in grains treated with the highest concentration (2000 mg/Kg)....

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Sultan Ahmed Maitlo2, Javeed Shabir Daar3 and Shahjahan Rajput

...osted by any unqualified labor

...
Hina Nazli, Abida Butt*
... Thorell, 1887 under laboratory conditions. The field rate of both insecticides caused approximately 50% mortality in the population of O. javanus. Toxicity data showed that these insecticides are slightly harmful ( caused < 80% mortality) towards studied spider. Insecticide residues of different ages were used to evaluate the residual toxicity of both insecticides. Mortality in exposed spiders decreased with the increased age of the resid...
Muhammad Usman Asif1*, Sadam Hussain Soomro2, Raza Muhammad1, Sikandar Ali Soomro2
...verpa armigera under laboratory conditions at their low, recommended and high doses by leaf dip method. The results concluded that significantly high toxicity was shown after treatment with Neomed 3 SC which caused 100 % to the insect at its recommended (200 ml) and high dose (400 ml) after 24 hours of application followed by Voliam Flexi 300 SC causing 66.66 % and 75% mortality on its recommended (80 ml) and high dose (160 ml), respectively. After 72 hour...

 Salman Khan, Irfan Ullah*, Shahid Ali and Murtaza

...land rent (Rs.15890) and labors having an estimated cost of Rs. 14965 respectively. The analysis showed that on average per acre yield of potato was recorded as 4,953.35 kg with net revenue of Rs. 28,261.89. The results of the Cobb-Douglas type production function revealed that the coefficients of seed, labor, tractor, urea and chemicals were positive and statistically significant having elasticities of 0.211, 0.110, 0.103, ...

Kifayat Ullah*1, Tahir Mahmood2, Naeem Jan3, Zeeshan Ali4 

A NOTE ON GEOMETRIC AGGREGATION OPERATORS IN T-SPHERICAL FUZZY ENVIRONMENT AND THEIR APPLICATIONS IN MULTI-ATTRIBUTE DECISION MAKING
...l fuzzy environment and elaborated with
a numerical example. The results obtained are analyzed and their advantages over existing structures are studied.

...

Mujahid Khan1*, Mohammad Tufail2, Muhammad Fahad1, Hazi Muhammad Azmathullah3, Muhammad Sagheer Aslam1, Fayaz Ahmad Khan4, Asif Khan5 

EXPERIMENTAL ANALYSIS OF BRIDGE PIER SCOUR PATTERN
...in the physical modeling laboratory of River Engineering and drainage control, USM, Malaysia.
The study shows that the pier scour depth and affected area around pier increase with the increase in pier size.
The study further demonstrates that the square pier models results in greater scour depth and area as compared to
circular pier models. Magnitudes of scour depths and area with reference to variation in shapes and sizes of piers
...

Daulat Khan1*, Khizar Azam2, Noor Muhammad Khan1, Muhammad Israr1, Naeem Ejaz3 

ASSESSMENT OF WASTEWATER FOR DISPOSAL IN ACCORDANCE WITH NEQS CRITERIA AND ITS REUSE FOR IRRIGATION WITH DILUTION

Mujahid Badshah, Saeed Badshah, Sakhi Jan 

HYDRODYNAMIC DESIGN OF TIDAL CURRENT TURBINE AND THE EFFECT OF SOLIDITY ON PERFORMANCE

Sajjad Hussain, Nisar Mohammad, Zahid Ur Rehman, Noor Mohammad, Ishaq Ahmad, Naseer Muhammad Khan, Salim Raza 

EXTRACTION OF GOLD AND SILVER BY CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA
...l, Khyber Pakhtunkhwa on laboratory scale testing. The research work was carried out into two parts. In first part the cyanidation parameters like grinding time, dosage of pH, cyanidation time, dosage of sodium cyanide (NaCN) and dosage of lime Ca (OH)2 were optimized. The samples were subjected to differential grinding time varying from 20 to 35 minutes, pH of slurry adjusted from 9 to 11 by adding Ca (OH)2 from 0.5 to 1.5 gm/kg , dosage of NaCN varies from...

Wisal Shah*, Muhammad Nafees**, Muhammad Iqbal***

EVALUATION OF MARBLE SLURRY WASTE FOR PREPARATION OF COMMERCIAL GRADE CEMENT
.... Cement was made in the laboratory according to standard formulation. Commercial brand available
in the market was used as control for product comparison. Physical and chemical properties of both cements and
mechanical properties of their concrete specimens were tested according to American Society for Testing Materials
(ASTM) standards. The test ...

 Muhammad Nafees*, Asim Nawab*, Wisal Shah**

STUDY ON THE PERFORMANCE OF WASTEWATER TREATMENT PLANT DESIGNED FOR INDUSTRIAL EFFLUENTS
...were then treated in the laboratory in order to
identify and suggest suitable coagulant. For this purpose three different treatments .i.e. Lime, Alum and Alum+Lime
were applied. The optimum doses identified were 25, 35 and 26:13 ml respectively. The settling time was 25, 35 and
20 minutes respectively for lime, alum and alum+lime. The results revealed that the combine treatment (Alum +
Lime) effectively removed s...

 Mujahid Badshah*1, Saeed Badshah1, Sakhi Jan1

HYDRODYNAMIC DESIGN OF TIDAL CURRENT TURBINE AND THE EFFECT OF SOLIDITY ON PERFORMANCE

 Sajjad Hussain1*, Nisar Mohammad1, Zahid Ur Rehman1, Noor Mohammad1, Ishaq Ahmad1,

Naseer Muhammad Khan2 and Salim Raza1
EXTRACTION OF GOLD AND SILVER BY AGITATION CYANIDATION PROCESS FROM COMPLEX ORE OF SHOUGHUR AREA CHITRAL, KYBER PAKHTUNKHWA PAKISTAN
...rried
out at laboratory scale. The three representative samples ground at time 25, 30 to 35 minutes and roasted at 650 oc
for 1 hour were subjected to agitation cyanidation process at varying dosage of pH from 10 to 11.13 , CaO from 0.5
to 1.5 gm/kg, NaCN varies from 1 gram to 5.93 gram and cyanidation time varies from 0 to 39 hours. The maximum
grade of 71.82 ppm with 99.03% extraction/recovery of gold and 34.34...
Naeem Ejaz*, Daulat Khan**, Usman Ali Naeem*, Muhammad Ali Shamim*, Muhammad Fiaz Tahir*, Faisal Shabbir*, Jawad Hussain*
DETERIORATION OF ORDINARY PORTLAND CEMENT AND FLY-ASH BASED GEO-POLYMER CONCRETES DUE TO SULFATE ATTACK UNDER THE ACTION OF SULFURIC ACID AND WASTEWATER
...arch work highlights the laboratory results of fly ash based geo-polymer concrete and effects on its durability under sulfuric acid exposure. Class ‘F’ fly ash was used in the preparation of samples that were properly cured for 28 days at room temperature. After curing, specimens were immersed in three types of sulfate containing solutions to check corrosion.. These sulfate containing solutions include static sulfuric acid, dyna...

Mumtaz Shah*, Hashim Nisar Hashmi* Naeem Ejaz* Abdul Razzaq Ghumman*

PERFORMANCE EVALUATION OF AQUATIC MACROPHYTES FOR MUNICIPAL WASTEWATER TREATMENT
...treatment. A bench scale laboratory model was developed and used for experimentation. Six experimental runs were conducted and each run laps over thirty (30) days. In each experimental runcontinuous monitoring of municipal sewage was done for each macrophyte specie. The aquatic macrophyte species used for the performance comparison were Water Hyacinth, Duckweed and Water Lettuce. Forperformance comparison four parameters including BOD5, COD...

Syed Mufeed Hadi Naqvi1*, Badar Naseem Siddiqui1, Waqar-Ul-Hassan Tareen1, Muhammad Ameer Qarib Naqvi2 and Naseeb Hussain3

Usage of Mobile Phone by Vegetable Growers and its Impact on Vegetable Production
...able growers with the collaboration of telecommunication agencies about the applications of mobile handset that will help the grower to increase their production. There is a need to provide trainings to the vegetable growers about using of mobile phone for obtaining reliable and authentic information about vegetables producing for enhanced production.

...

 Saman Shahid1, Shahid Ali1, Muhammad Abdullah Ahmad1, Sarfraz Munir2

RE-DESIGNING AND OPERATIONAL ANALYSES OF THE SEDIMENT TRANSPORT CONTROL IN IRRIGATION CANAL NETWORK
...etwork is a tiresome and laborious work, where engineers spend months to design canals. Over the years, engineers and agriculturists in the Punjab have been trying to find a solution to the sediment transport problem which occurs in existing irrigation system. To address these problems, the canals can be analyzed to point out the flaws in design and operation mechanism. This study used a Physics based mathematical model, which can aid the engineers to design a...

 Riaz Sayed1, Khizar Azam1, Waqar Shah1, Inayat Babar1, Hamidullah1, and Sahar Noor1

A REVIEW OF DRIVING UNDER THE INFLUENCE OF FATIGUE

Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5

Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
...cessed based on standard laboratory protocols. An overall Mycoplasma bovis isolation rate of 0.83% (4/480) was obtained. Based on the states studied, 1 (0.35%) and 3 (1.53%) M. bovis were isolated from Adamawa and Taraba States, respectively with an insignificant association between M. bovis infection and the states sampled (P>0.05). Based on organs/site sampled, 2 (5.40%) isolates of M. bovis were from lung tissues and 1 (2.70%) were from both pleural flui...

Hina Imtiaz1*, Salma Khalid2, Kashaf Riaz1, Muhammad Arshad Ullah3 and Zubair Rehman

...ples were treated in the laboratory for identification and recommending suitable coagulant. Three different treatments were practiced i.e. Alum, Lime and Alum+ Lime. For alum, lime and alum + lime the settling time interval was 40, 35 and 20 minutes respectively. Results revealed that the combine treatment (Alum + Lime) effectively reclaimed Total Suspended Solid (TSS) up to 85%, along with 80% BOD, and 75% COD. Waste management is core issue that affects the ...

Uzma Arif1, Sadar Uddin Siddiqui2, Muhammad Fareed Khan1, Muhammad Arshad3 and Shakeel Ahmad Jatoi

Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...ar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae of Lab-S and GUW populations when compared with their r...

Muhammad Ismail1, Tufail Ahmad2, Shamsher Ali2*, Shahid Ali1, Nur Ul Haq1 and Naveedullah3

Heavy Metals (Pb and Ni) Pollution as Affected by the Brick Kilns Emissions
... m high from ground. The laboratory analysis of wheat leaf samples showed that the concentration of  Pb and Ni in chimney A and B as 2.90 and 1.8, 13.11 and 14.7 mg kg-1 respectively. The concentration of Pb and Ni at this stage is not in toxic range however with the continuous addition may pose a severe threat in future. Similarly, soil sample analysis revealed AB-DTPA extractable Pb and Ni for chimney A and B as (0.11- 0.13, 14.2 and 15.0 mg kg-1) respe...

Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2

A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...6%); followed by skilled labor (handicrafts: 28%), and agriculture (12.1%). 

...
Shahid Ali*, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram and Imbasat Yousaf
..., subcutaneous edema and labored breathing.
...

Shaiza Rasool1, Iftikhar Ahmad1*, Khurram Ziaf1, Irfan Afzal2, Muhammad A.S. Khan1, Muhammad Aashir Sajjad1 and Muhammad Zain Ali1

Seed Conditioning Effects on Germination Performance, Seedling Vigor and Flower Production of Zinnia elegans
...gans Jacq.). Two initial laboratory experiments evaluated the timing and frequency of seed magnetic stimulation to select the most promising treatments for field production testing and flowering. In the first experiment, a 3% moringa leaf extract (MLE) showed the earliest (2.8 d) and the highest germination rate (90%) with an average root length of 4.2 cm. Hydro-priming resulted in the largest seedling fresh weight (0.8 g), seedling shoot length (5.8 cm), and ...

Moazam Ali1, Wajid Ali2, Ayhan Ceyhan2 and Zeeshan Ahmad Bhutta3*

Pigmentation Genome Influence in Animals and Human Interventions in its Course of Action
...cle contains a complete elaboration about the worth of pigmentation in animals, natural pigmentation process and new mutation and deviation in animal genome related to their pigmentation for human and animal welfare respectively.

...
Muhammad Yaseen1*, Xu Shiwei2, Yu Wen2, Ejaz Ashraf1, Muhammad Luqman1, Muhammad Ameen3 and Sadia Hassan4
Farmers Behavior to Participate in Public Sector Agricultural Extension Services: Comparative Analysis of Pakistan and China
...uence of age, education, labor, farm area and villages on the household head’s participation in agricultural extension services/ activities organized by public sector department. For this purpose, a random sample of 160 households from four districts of Punjab province of Pakistan was selected, and another random sample of 122 households selected from the six villages of Huailai County of Hebei Province in China. Data were collected using the interview m...

Md. Sahidur Rahman

Origin and Spillover of Coronaviruses: Prospects of One Health Action
...plement multisectoral collaborative action among physicians, veterinarians, wildlife experts, epidemiologists, environmental scientists, and microbiologists to curb the COVID-19. One health encourages joint disease surveillance by establishing laboratory networks and regulating proper biosafety, biosecurity, and risk communication to facilitate strong disease prevention and control strategy by which we could prevent the next...

Sajid Amin Sarmad1, Muhammad Zeeshan Majeed1*, Muhammad Luqman1, Muhammad Asam Riaz1, Sohail Ahmed3 and Sylvain Nafiba Ouédraogo2

Development and Laboratory Evaluation of a Slow Release Formulation of Fipronil against Subterranean Termites (Odontotermes obesus Rambur)
...duals of O. obesus under laboratory conditions using soil macrocosms. Mortality of termites was determined at different time intervals after their exposure to formulated pellets treated macrocosm soils. Results revealed that fipronil formulated with Z. mays substrate remained more effective for longer period of time against subterranean termites as compared to that formulated with S. officinarum bagasse material. The maximum mortality of termites was observed ...
Ammara Gull-E-Fareen1, 3, Imran Bodlah1*, Muhammad Tariq Rasheed1, Yasir Niaz2, Muhammad Adnan Bodlah2, Muhammad Asif4 and Nasir M. Khokhar5
...93) and Tetraponera allaborans (Walker, 1859). A lot of surveys were conducted during 2015-17 for the collection of ants associated with aphids on different host plants in different localities of Pothwar region. As a result of surveys, ant-aphid new mutualistic trophic associations were determined like Camponotus compressus with 12, Formica fusca 1, Formica clara 2, Lepisiota frauenfeldi 9, Myrmica aimonissabaudiae 4, Tapi...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...lty Statement | This laboratory study demonstrates the bioefficacy of indigenous plant extracts and essential oils as safe and eco-friendly alternates to hazardous synthetic insecticides and suggests the incorporation of these indigenous phytoextracts in the future bio-intensive pest management programs ag
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
..."font-size: small;">This laboratory study encompasses comparative evaluation of insecticidal potential of indigenous ethnomedicinal flora of Soone Valley and surrounding Salt Range of Pakistan. Acetone extracts (10%) of forty plant species were evaluated against Asian citrus psyllid (Diaphorina citri), armyworm (Spodoptera litura), house mosquito (Culex quinquefasciatus) and subterranean termite (Odontotermes obesus) using twig-dip,...
Muhammad Ramzan, Unsar Naeem-Ullah*, Mudssar Ali and Hasan Riaz
...amina (L.) under the laboratory conditions. The results on different biological and morphological parameters showed that the fecundity of female ranged from 160 to 281 which increased its survival rate. Trilocha varians has five larval instars. The last instar changed its colour to dark reddish and look like branches of host plants. The male and female mean longevity was 6.0 ± 1.171 and 11.4 ± 1.70 days. Pale reddish brown lines were p...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
...the spectrophotometer in laboratory. The level of zinc in cancer patients was observed lower than the optimum value. Male have much lower concentration as compare to females, therefore male are more susceptible to cancer than female. In the nutshell Zinc is an effective anti-inflammatory and antioxidant agent, including as a natural cancer treatment.

...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

...fied and isolated by our laboratory. Alarmingly, antimicrobials in animals are inappropriately using and resultantly leading to antimicrobial resistance (AMR) including mycoplasmas, which are causing serious infections in ruminants. Since the growth curve of our local strain and its susceptibility to commonly using antimicrobials is unknown. Therefore, this study was aimed to evaluate the growth curve and susceptibility of Mccp against commercially available q...

Muhammad Yasir*, Mansoor ul Hasan, Muhammad Sagheer, Amer Rasul, Rameesha Amjad Ali and Habib ur Rehman

Evaluation of Spinosad Applied to Grain Commodities for the Control of Stored Product Insect Pests
...0.50 and 1 mg Kg-1 under laboratory conditions maintained at 28 ± 2oC, 65 ± 5% RH and continuous darkness. Seven bioassays were conducted by releasing the insects on treated commodities after different post treatment periods (0,1, 2, 3, 4, 5 and 6 months). The mortality of three insect species was recorded at tested concentrations in all the treated commodities after the exposure period of 3 and 7 days. Overall results of all bioassays show that ...

Sajid Khan* and Shahnaz Akhtar

Effect of Farm Labor Transformation on Households Income in Central Districts of Khyber Pakhtunkhwa, Pakistan
...ssess the effect of farm labor transformation on households’ income in central three districts of Khyber-Pakhtunkhwa Province of Pakistan. Data on different aspects of farm labor transformation were collected through a well-designed and pre-tested questionnaire. Nine village councils from three districts were selected through random sampling techniques. The data were collected from 393 sample households and were analyz...
Yonghua Liu*, Xianhua Li, Xiongfei Yan and Gang Li
...es in North China. Under laboratory conditions (temperature 25 ± 1ºC, 80 ± 5% relative humidity, photoperiod 14L:10D), the effect of the 4 host plants Armeniaca vulgaris (apricot), Malus pumila (apple), Prunus salicina (plum), and Amygdalus persica (peach) on the growth, development, survival, reproduction, and life table parameters of A. fimbriana were studied. Different host plants had significant effects...

Mohammad Adil1*, Muhammad A. Khan2, Naveed Ahmed1, Edward Bromhead3, Muhammad A. Arshad1, Muhammad Fahad1, Shahid Ullah1 and Khan Shahzada1

Numerical Investigation of the Effect of Column Staggering in Flat Slabs Systems
...flat slab may reduce the labor as well as the material cost of the structure without affecting the structural performance of the slabs as well as columns. Wide parking and halls could be constructed and architects will have more freedom and flexibility in their designs with reduced reliance on structural design. This study is focused on the basic flat slab analyses and design with the help of finite element software along with manual method of analyses and des...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...treatment and brought to laboratory for qualitative and quantitative analysis. Direct smear method and fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were identified from Jallo Wildlife Park. Eimeria (7500) and Ascaris (1100) were the most abundant. M...
Mohsin Ali1, Nargis Haider Kakar2, Rida Dawood3,4, Qurat-ul-ain Fatima5, Zunera Tanveer2, Shoaib Ahmad Malik6, Tauseef Muhammad Asmat1, Asadullah1, Muhammad Masood Tariq Kiani1 and Mohammad Zahid Mustafa1*
... study, undertaken in collaboration with Shaikh Khalifa-Bin-Zayed Hospital Quetta, investigates the relation of thyroid abnormalities in 224 DM (both type 1 and type 2) patients. The parameters of age, duration of diabetes and type of treatment were measured. Venous blood was taken to investigate the thyroid profiles. Out of 224 patients examined, 67.85% showed the normal thyroid profile and thyroid abnormalities were seen in 32.15% of subjects. There were 10 ...
Sana Mehmood1, Hafiz Muhammad Tahir1*, Muhammad Summer1, Sher Muhammad Sherawat2, Shaukat Ali1*, Sajida Naseem3
...They were brought to the laboratory for subsequent morphological identification, DNA extraction and sequencing. We identified 38 morph-species, representing 22 genera and 8 families. Accuracy of morphological identification was confirmed by barcode analysis of tissue samples. A standard barcode sequence of COI (Cytochrome c Oxidase I) was recovered from 90 specimens of spiders. Percentage accuracy of morphological identification was 92.10%. Four morphologicall...

Muhammad Shahid1*, Unsar Naeem-Ullah1, Waheed S. Khan2, Shafqat Saeed1 and Kashif Razzaq3

Application of Nanotechnology for Insect Pests Management: A Review
... large ‘‘bio-laboratory” comprising of plants, algae, fungi, yeast, etc. which are consists of wide array of biomolecules. There is various size and shapes of nanoparticles to be synthesized by using the naturally occurring biomolecules. These biomolecules acting as a driving force for the designing of greener, safe and environmentally benign protocols for the synthesis of nanoparticles. Insect pests are main density dependent factors that de...
Eslam Moradi-Asl1*, Behnam Mohammadi Ghalehbin2, Kamran Akbarzadeh3, Jafar Mohammad-Shahi4, Hajar Afshin4

Hafiz Muhammad Wasim1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Ayesha Zafar1, Imran Shehzad1, Aneela Riaz3, Abid Niaz2, Khurshid Ahmad Mufti4 and Muhammad Arif2

Deleterious Impact of Sodium Chloride (NaCl) Toxicity on Chemical Properties of Soil and Ionic Composition of Rice
...nt pots and subjected to laboratory for determination of various chemical properties (pH, EC and SAR). To calculate SAR values of soil, concentration of Ca2+, Mg2+ and Na+ were also determined from all soil samples. It was observed from the results that addition of NaCl proved toxic for different chemical properties of soil as values of pH (7.23 to 8.44), EC (2.96 to 13.54) and SAR (2.45 to 11.57) were impaired negatively and in systematic manner with the incr...
Tariq Mahmood Khalil1,2*, Muhammad Ajmal3, Iftikhar Zeb1,4 and Muhammad Azeem Khan1,5
 
 
...ter for irrigation. This laboratory scale study was aimed to investigate the effects of saline water irrigation on development of total water potential within coarse and fine textured soils using a novel method based on water potential dynamics. Chilled mirror dew point hygrometer accurately captured the water potential dynamics when soil samples were irrigated with saline water. The instrument output showed that soil texture has profound effect on soil total ...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
Riffat Sultana1*, Nuzhat Soomro2, Santosh Kumar3 and Ahmed Ali Samajo2
...In trials carried out in laboratory we verified the following mating sequence: (1) sexual recognition by antennation; (2) courtship with male turning his abdomen towards the female performing mediolateral antennae vibration and jerking its body antero-posteriorly. We quantified elapsed time of each behavioral sequence i.e. age of maturation, duration of pre-copulatory and mating, interval between each mating, length of copulation, and number of mating i...
Salman Ali1*, Ayaz Ali2, Riaz Ali Rind2, Majid Ali3, Zulfiqar Ali Mastoi2, Shagufta Naz3, Muhammad Shakir3, Rashid Ahmed Qaim Khani1
...ut during the 2019-20 at laboratory of Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam. The treatments included in the study are: Grilled fish T1=, Fried fish T2=, Microwave T3 = respectively, and treatment were arranged in CRD with the repeats. The results revealed that the cooking in methods were significantly varied (LSD<0.05) for different parameters. The fish cooked in microwave resulte...
Suwati Suwati1, ErniRomansyah1,*, Syarifudin Syarifudin1, Yahya Jani2, Agus Heri Purnomo3
Damat Damat4 and Erkata Yandri5,6
...entally in the field and laboratory. And the data were analyzed using simple linear regression to formulate a trend of reduction in the weight of seaweed while drying. The results showed that the weight reduction in seaweed by the natural method can be illustrated by the simple regression linear equation Y = -2.385 3x + 943.65 with R² = 0.985 9, which means a reduction in weight of seaweed i.e. 2.385 3 g min–1 during 340 min. While...
Ahmed Saud Alsaqufi1, Sheikh Mustafizur Rahman2,3*, Roshmon Thomas Mathew2, Yousef Ahmed Alkhamis1,2, Md. Moshiur Rahman3,4 and Muhammed Aslam Pathiri2
...can catfish larvae under laboratory condition. Newly hatched larvae were stocked in plastic aquaria (10L) at a rate of 5 individuals/L and reared for one month under four treatments such as 24h light (24L), 24 h dark (24D), 12h light and 12h dark with PVC (12DL_PVC), and 12h light and 12h dark without PVC (12DL) conditions. A total of 108 larvae were sampled for phenotypic traits analysis. The results revealed that complete darkness (24D) significantly improve...

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sher Muhammad3, Muhammad Latif4, Ayesha Zafar1, Imran Shehzad1, Sarfraz Hussain5, Aneela Riaz6 and Abid Niaz2

Muhammad Fiyyaz1, Ghulam Sarwar1*, Noor-Us-Sabah1, Mukkram Ali Tahir1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Sarfraz Hussain3, Ayesha Zafar1, Imran Shehzad1 and Aneela Riaz4

Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...gical examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, CI=28.45-48.79), 15.6% MRSA (5/32, CI=6.87-31.76), 46.9% haemolytic S. aureus (15/32, CI=30.87-63.56) and 34.4 % biofilm producing S. aureus (11/32, CI=20.41-51.69). Earthen floor type (OR=1.75, p=0.0996), poor drainage system (OR=7.33, p=0.002), pond as sour...
Suman Bhattarai1*, Subodh Raj Pandey2, Jaya Prakash Dutta3, Meghnath Timalsena4 and Rajendra Bam5
...del revealed that family labor (p=0.68), age(p=0.007), family size (p=0.001), experience (p=0.007), harvesting (p=0.000) and maintenance of flowering plants (p=0.057) have significant effect on honeybee productivity. Labour cost and migration cost had positive coefficient and significant relation at 1% level of significance with gross return whereas expenses on baiting materials had positive coefficient and significant relation at 5% level of significance with...

Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2

...ation of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1% fungal inhibition. The remaining 2 fungicides Melody Duo and Aliette were least effective by showing 48.2% and 37.5% fungal inhibition, respectively. In vivo experiment from all four fungicides, Cabr...

Shahzad Khan1*, Munir Khan1, Arif Alam2, Ikram Shah2, Mahfooz Khan1 and Fida Muhammad Khan1

...conomic analysis and to elaborate the determinants of wheat crop in district Charsadda, Khyber Pakhtunkhwa, Pakistan in the year 2018. Three villages, namely, Aspandehri, Kamran Kalay and Sarfaraz Kalay, were purposively selected. A sample size of 41 wheat growers from farm households was chosen from these villages for data collection. Thus, selection of the farmers was based on the proportional allocation technique. However, primary data were randomly collect...

 Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*

...aintained in
laboratory colony, leads to the detection and isolation of this virus from
Egypt. Injection of filtrate induced SGH symptom in 60% injected flies.
The salivary gland symptom was similar to the previously reported
symptoms of the MdSGHV. Examination of the SGH suspension with
Electron microscopy revealed the presence of rod-shape virus particles
wit...

Ghulam Murtaza1*, Muhammad Ramzan2, Assad Ullah3, Abid Ali5, Ayesha Zafar3, Rukhsar Beanish3, Ahmad Ali4, Ghulam Mustafa4 and Mudassar Aslam4 

...n different fruits under laboratory conditions. Infested fruits were collected from local fruits and vegetables market and brought to laboratory. Collected fruits and vegetables were placed in cages having sand in bottom for pupation. After the adult emergence fruit fly species were identified based on diagnostic morphological features and placed in separate cages. Different fruits such as guava, apple, fig, banana and citru...
Ala’audin Hakami,1AusamaA Yousif,2* Mohamed Zaki,3 Mahmoud Ismail,4,5 Ahmed Al-Ali,5 Abdu-Rahman Al-Ankari5 
...asingly reported without laboratory
diagnostic evidence of virus circulation. The objectives of this study were
to investigate the presence of PBFDV in the Arabian Peninsula, and to
identify its possible molecular origins. Total DNA was extracted from
feather and blood samples from randomly selected 19 clinically-suspect
and 153 apparently-normal birds collected from 10 locally-bred and
im...

Nashwa M. Helmy1 and Ahmed S. A.2

...spectively) submitted to laboratory
examination by Priochek for NSPs (3ABC) 32 out of 70 were positive (12 sera samples
from Sharkia and 20 sera samples from Fayoum), while 22 out of 70 were positive by
real time RT-PCR (10 sera samples from Sharkia and 12 sera samples from Fayoum).
Definitive diagnosis of foot and mouth disease requires the detection of virus antigen or
genome. So, the vesicular epit...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...d even in low technology laboratories. The amount of tissue
required by this method is ∼50–100 mg. The quantity and the quality
of the nucleic acid extracted by this method is high enough to
perform hundreds of PCR-based reactions and also to be used in
other DNA manipulation techniques such as restriction digestion,
Southern blotting and cloning.
...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...ol for use in veterinary laboratories. Thus it will enhance the detection and
characterization of the circulating EHV-1 in Egypt. Control strategy for EHV-1 viruses in vaccinated
and non-vaccinated animals needs to be addressed.
...

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

...esults obtained from the laboratory-based techniques
such as (DAS)-ELISA, and RT-PCR using a pair of primers specific to the AMV-CP gene.
Phylogenetic analysis results indicated that AMV-Egypt that isolated from Basil is most closely
related (96.4%) to the AMV-Spain strain isolated from Hibiscus plants.
Conclusion: Pathological investigation may provide insights into the alterations of the cell after
...

Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2

...ar2 IB) vaccine based on laboratory tests and
repetition of challenge test.
Methods: We prepared formalin inactivated combined vaccine for Avian Influenza (H9N2N),
Newcastle (La), and IB (M41 and Var 2) by using montanide 71 adjuvant. The prepared vaccine was
injected intramuscularly in specific pathogen free and commercial layer chicken, the injection was
repeated as a booster vaccination after a mon...

Bakhtiar Gul*, Saleetha Rabial and Haroon Khan

...ble, economical and less laborious for the management of duckweed for large scale infestation.

...

Muhammad Rafique1, Muhammad Azhar Iqbal2, Inam Ul Haq1*, Muhammad Ramzan Anser2, Humara Umar2 and Muhammad Ashraf Sumrah2

...(Pvt.) Ltd. under the collaboration with Barani Agricultural Research Institute (BARI) Chakwal to find out the appropriate light intensity for successful olive propagation under greenhouse conditions. The data regarding days to root initiation, number of roots, root length, days to shifting, rooted cutting percentage and callus percentage were collected under four different treatments. Cutting under the only green net (5944 lux), white net (11428 lux) only upp...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...cally and transported to laboratory within 4-6 hs of collection in cooling conditions. The samples were examined for Salmonella contamination; isolation and identification of Salmonella serovars was done using the standard procedure, while antimicrobial profile was illuminated using the disk diffusion method.

Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2

...asal rot of onion in the laboratory, greenhouse and field. Completely randomized design (CRD) was used for in-vitro study and randomized complete block design (RCBD) for greenhouse and field experiments. Three desert plants i.e. Gossypium thurberi, Calotropis procera and Suaeda fruticose were used at 50 ppm, 100 ppm and 150 ppm concentrations. Maximum basal rot disease reduction (78.6%) was recorded at 150 ppm concentration of wild cotton (G. thurberi) under i...
Arbab Zubair Ahmad1, Farman Ullah1, Hayat Badshah2, Muhammad Shehzad Khan1* and Bashir Ahmad1
...chogramma chilonis under laboratory conditions at Agriculture Research Institute (ARI), Tarnab, Peshawar-Pakistan during, 2017-2018. The cereals (maize, oat, wheat and barley) were evaluated on the basis of total adult emergence, egg laying ability, developmental time of S. cerealella and enhancing parasitism along with sex ratio of T. chilonis raised on eggs from moths reared on cereals. The response of cereals to S. cerealella in no choice test revealed sign...

Sajida Batool1, Saira Batool1, Sitara Shameem1, Fatima Khalid1, Tahira Batool2, Summera Yasmeen1, Saima Batool3*

...s in the liver of albino laboratory mice, 20 adult male mice were equally distributed (n=10) into two groups. Atrazine was given at the dose of 200 mg/kg body weight through gavages for 28 days while the control (Cnt) group remains untreated. On the 29th day, all animals were sacrificed to recover liver for histological preparations. Atrazine (ATZ) caused various hepatic implications in the treated animals. ATZ exposure caused a significant decline in mean bod...
Yuzhou Luo1, Ping Wang1, Kaijun Yu2*, Song Luo3 and Longjie Sun4
...s data acquired with our laboratory-made sleep monitoring system. Furthermore, a commercial system was compared for validating the efficiency of our proposed algorithm. This work is the first study that successfully used breath sounds to classify sleeping conditions. The experiment validates the effectiveness of the proposed algorithm.
...
Ahmad Wahyudi1*, Sujono Sujono2, Listiari Hendraningsih2, Ari Prima2, Zane Vincēviča-Gaile3 and 
Ivar Zekker4
...erformance is yet to be elaborated. Therefore, the effect urea addition on TMR and its silage on performance of Friesian Holstein (FH) male calves were evaluated. The calves (n = 27; 5 mo to 7 mo, mo = month old) were divided into three groups, each group consisted of nine calves, based on age: 5 mo (100 kg to 105 kg), 6 mo (111 kg to 116 kg), and 7 mo (123 kg to 133 kg). All groups were divided into three types of feed, namely: conventional feed containing co...
Li Qiao1,2, Quan Zhang1, Shubao Geng1,2, Fangmei Zhang1,2, Junhua Chen1,2 and Shibao Guo1,2,*
...ren were investigated in laboratory. The results showed that the total egg quantity, oviposition rate and adult longevity were all affected. The egg number every female adult of E. grisescens Warren was reduced 37.36%, 26.17%, 41.88% and 35.11% contrast to the control. The average oviposition rate reduced 16.61%, 11.44%, 11.81% and 8.86% respectively with the supply of four wave lengths at night. Meanwhile, the pre-oviposition period and oviposition per...

Shahid Ali1*, Murtaza1, Waqas Ahmad1, Muhammad Israr2, Aftab Khan3, Hamdullah1 and Syed Attaullah Shah1

...at 1 percent increase in labor hours and urea increased yield by 0.411% and 0.231%, respectively, at 0.01 level of α (p-value = 0.000). A percent increase in number of irrigation and tractor hours increased yield by 0.110% and 0.044%, respectively, at 0.05 level of α while chemicals increased yield by only 0.029% but was statistically insignificant. Summing up all the estimated coefficients of the regressed model resulted a value of 0.8266, implyin...

Hamida Bibi1*, Dave Rraffaelli2 and Muhammad Sharif1

...ies as determined in the laboratory by Average Score Per Texon (ASPT) as well as water conductivity. For most sites the body mass distributions were skewed towards smaller body sizes with another apparent mode for large size class. Contrary Kernel Density Estimates (KDE) for body mass spectra revealed marked differences in modalities among the sites. The distributions of body masses in cleanest and polluted sites were inversely related with water quality while...
Madiha Nawaz and Shoaib Freed


...>P. solenopsis under laboratory conditions by immersion method. Three entomopathogenic fungi; Beauveria bassiana (isolates Bb-01, Bb-08), Metarhizium anisopliae (isolates Ma-11.1, Ma-2.1) and Isaria fumosorosea (isolates If-2.3, If-02) showed percent mortalities of (61.0%, 85.0%), (78.0%, 56.0%) and (52.0%, 54.0%) with LC50 values of (4.25×108, 2.54×108 spores/ml), (3.26×108...

Salma Javed1* and Daniyal Siddiq2

... javanica, in tomato. In laboratory conditions second-stage juveniles (J2) mortality was recorded after 24, 48 and 72 h of incubation in concentrations of 0.25, 0.5 or 1%. Maximum mortality was achieved with ethanol extracts followed by aqueous and methanol extracts. In the greenhouse, leaf extracts at 60 days after inoculation produced reduction in root galling, egg masses, and nematode populations in plants in treated containers compared to untreated plants ...

Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1

...t-align: justify;">Under laboratory conditions, aqueous, acetonic and methanolic extracts of Solanum nigrum, Atropa belladonna, Hyoscyamus muticus, Capsicum frutescens, Datura innoxia and Withania somnifera were tested against root-knot nematode, Meloidogyne javanica. All the tested materials affected the survival of the nematode juveniles and egg-masses hatched depending on materials property, concentrations and solvents used in extraction. The aqueous extrac...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...ifferent bacteriological laboratory methods and purified isolates were identified according to gram’s staining reaction, colony morphology, cultural characteristics, biochemical and antibiotic susceptibility test. All most 100% dried and 20% cooked fish sample were contaminated. In this study seven different species and 168 isolates were identified from dried fish and these were Escherichia coli 21.43% (36), Vibrio spp. 18.45% (31), Staphylococcus spp.17...

Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah

...naceous vegetables. This laboratory work was aimed to determine the comparative toxicity of three differential-chemistry insecticides (i.e. emamectin benzoate 1.9 EC, spinosad 240 EC and lufenuron 50 EC) and the aqueous extracts of three local plant species (i.e. Eucalyptus camaldulensis Dehn., Citrus limon L. and Azadirachta indica A. Juss) against the grubs and adults of E. vigintioctopunctata. Three concentrations of each synthetic insecticide (i.e. 2.5, 2 ...

Atef El-Sagheer1*, Anas El-Mesalamy1, Abdel-Monem Anany2 and Nashaat Mahmoud1

...vanica were estimated in laboratory and greenhouse conditions. Results revealed that all the tested culture filtrates caused significant reduction in number of J2 in all treatments, compared to the non-inoculated control, where the hatching inhibition and mortality percentages increased with an increase in exposure time. At 24 hours, increased the mean number of J2 hatched slowly than in first stages of experiment. Similar patterns showed against M. javanica i...

Yaregal Tilahun1*, Benyam Tadesse1, Getachew Mekonnen2  and Tilahun Bekele3

...las model indicated that labor force, fertilizer, land size, and improved seed all had an impact on tea productivity. While, technical inefficiency of tea production was determined by to level of education, the interaction of extension contact, distance to district market, harvesting time, training, and participation to off/non-farm income. So, improving the skill and knowledge of farmers through increasing extension service and training, on-time delivery of f...

Samreen Khan*, Salma Javed, Tabassum Ara Khanum, Nasira Kazi

...ent research effort was elaborated to conduct surveys and to ascertain the evidences of entomopathogenic nematodes from one of the southern districts i.e., Lakki Marwat, Khyber Pakhtunkhwa province of Pakistan.
...

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

...sions were collected for laboratory analysis. Laboratory analysis including bacterial culture and biochemical tests were done to identify the causal agents of SCM and udder lesions. The overall prevalence of SCM and udder lesions were 56.8% and 32.1%, respectively. Most of the farms under study were small scale (94%) except a few were medium-scale (6%). Very few farms used quarantine and isolation shed (6%) for the disease a...
Syed Sikandar Habib1*, Saira Naz2, Sadia Nawaz1, Iqra Ameer1, Ameer Khatoon1, Hameed Ur Rehman3, Sahibzada Muhammad Jawad4 and Haider Ali5
...ing boxes and carried to laboratory for total complete blood count. The water parameter was analyzed in laboratory by taking the sample of water and some (Temperature, DO) was measured in situ. Results of hematological analysis revealed that there is no significant (P > 0.05) difference in packed cell volume, mean cell volume and mean cell hemoglobin of different selected species adapted to similar environmental condition...

Muhammad Waryam Warraich1, Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

...lowed by analysis in the laboratory. It was observed that use of press mud @ Yield 1 % in integration with SOP as chemical K fertilizer performed as best treatment by improving yield (4.11 t/ha) and yield contributing parameters like maximum plant’s height (95.10 cm), length of spike (11.04), number of fertile tillers (5.67), 1000 grains weight (39.26 g) and grain count per spike (51).

...

Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1

... conventional ones. This laboratory study comparatively evaluated some selected differential chemistry insecticides against some destructive and economically important insect pests i.e. mango mealybug (Drosicha mangiferae Green), mango leafhopper (Idioscopus clypealis Lethierry), Asian citrus psyllid (Diaphorina citri Kuwayama) and subterranean termites (Odontotermes obesus Rambur). Standard twig- and filter paper-dip bioassay methods were used against sap-fee...
Stefan Pratama Chandra1, Yoanes Maria Vianney1, Theresia Liliani Christie2, Merlyn Wongso2, Melisa Widjaja2, Deok-Chun Yang3, Se Chan Kang4, Manar Fayiz Mousa Atoum5,6 and Johan Sukweenadhi1*
.../b>atory (KUH Lab), a collaboration initiated to achieve the independence of raw materials. This laboratory is devoted to developing the plant tissue culture protocol for the mass production of P. ginseng root cultures. This paper is the first report of in vitro P. ginseng culture in Indonesia. The initial stage of the mass production focused on optimizing the culture conditions: inoculum weight (100 g, ...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

...nd that during a 12-hour laboratory study, A. cybister consumed 10 exposed larvae. Under field conditions, introduction of predator decreased the larval density from 141.7 to 71 in 15 days. In conclusion, these plants and predator may be useful in controlling mosquito populations in an eco-friendly way.

...

Muneeb M. Musthafa1,* and Fauziah Abdullah1,2,3

...pical mountains are good laboratories to assess beetle diversity which can be utilized for climate change studies and ecological disturbances. Long term monitoring program between different mountains on beetle diversity and distribution would enhance the understanding of their habitat pattern in relation to seasonal pattern and anthropogenic disturbances.

...
Zane Vincevica-Gaile1, Karina Stankevica1, Maris Klavins1, Roy Hendroko Setyobudi2, Damat Damat3*, Praptiningsih Gawawati Adinurani4, Lili Zalizar5, Muhammad Zul Mazwan6, Juris Burlakovs7
Didiek Hadjar Goenadi8,9, Rista Anggriani3 and Aamir Sohail10
 
 
...s biomass ashes for the elaboration of peat-free soil-improving products. As a case example, may serve a description of the peat-free product made by rotary drum granulation from biomass fly ashes (energy production waste) and local freshwater sediments in a mass ratio mixture of 67:100, optimally applicable for soil improvement at a rate of 50 g L–1. Besides, regional opportunities in Indonesia and Latvia are referred. It was conclu...

Martha Echioda Ogbole*, James Agbo Ameh, Samuel Mailafia, Olatunde Hamza Olabode and Bridget Jessica Adah

... were confirmed based on laboratory confirmation of LV- transcription polymerase chain reaction (LASV-RT- PCR). The number of suspected and confirmed cases as well as total deaths and case fatality rates in the years under review were collated. Out of the total number of 17,777 suspected cases, 2959 cases were confirmed positive for LF by a laboratory test, while 781 deaths were recorded. Annual distribution of LF showed sig...

Murat Kulekçi1, Ecevit Eyduran2, Ayaz Yusuf Altın2 and Mohammad Masood Tariq3*

...asian and others), were elaborated. In MARS algorithm, no over-fitting problem was observed under a set of explanatory variables consisting of number of full beehives and total honey production, whereas Bagging MARS captured the interaction effects of number of full beehives with educational degree and other working areas. The constructed MARS and Bagging MARS models produced a marvelous fit in the prediction of honey yield per beehive. It was concluded that b...

Noura El-Shahat Attia1*, Abd El-Khalek Ramadan El-Sheikh1, Mohamed Omia Siam2 

...cording the clinical and laboratory findings of Zinc (Zn) deficiency in dogs. The clinical study was conducted on two groups of dogs. The first group consisting of seven dogs which was clinically healthy and used as a control group, and the second group consisting of sixteen dogs “German Shephard” at different ages which was suffering from decrease appetite, stunt growth rate, as well as skin lesion in form of loss of hair, crust, scales and inflam...

Aasma Younas*, Ijaz Iqbal and Atif Akbar

...y, no one has time to do laborious work by hand. The results suggested some general rules for the construction of such designs. For p ≤ 5 and different combinations of n and p. All possible shifts will help the planners to enhance their choice.

...

Gülseren Kırbas Dogan1,*, Emin Karakurt2, Mushap Kuru3 and Hilmi Nuhoglu2

...e brought to the anatomy laboratory and anatomically examined. The most important result; in the mid-section of the sections taken from the existing structure other than the normal formation at the medulla oblangata and pons level in the brain, it was found that in the outer part of the meninges, an inversion of the substantia alba was formed in an outer layer, as if the substance was invaginated. As a result, various anomalies in 6 calves were evaluated accor...

Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1 

...t house fly larvae under laboratory conditions. Batches of house fly larvae (25 individuals per replicate and 4 replicates per dose of fungi) were subjected to five doses from each fungus (101, 103, 105, 107 and 109 conidia/ml) in plastic cups for one min then transferred to a clean one. Both test and control cups were incubated for 7 days to determine LC50, 90 and EI50, 90. Joint action of these fungi with three botanical oils (celery, ginger and sesame) as w...

Muhammad Salman*, Muhammad Hamayoon Khan, Muhammad Zahid, Gul Zamin Khan, Fazli Rahim and Usman Khalique

...ring of B. zonata in the laboratory due to its consistent availability. 

...

Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar 

...ter a detailed clinical, laboratory and ultrasonographic examination 21 IBD dogs with symptoms of chronic gastrointestinal diseases were chosen for the study. In addition to 11 healthy dogs served as control group. In comparison to controls, hematological analysis revealed significant variations (p<0.05) in total leukocyte count, hemoglobin, and mean platelet volume and significant variation (p<0.01) in neutrophils and platelet count. The biochemical ana...

Jan Clyden B. Tenorio

...One Health is truly a collaborative affair. The paradigm, by definition, is multidisciplinary, multi-sectoral, and integrative in nature. Thus, effective collaborations and partnerships towards understanding are integral to successful programs. Moreover, how a One Health institution thrives is heavily reliant on how the separate, yet interconnected branches effectively communicate to each other. The aim of this paper is to c...
Ilvir Khabibullin1*, Ruzel Khabibullin1, Irina Mironova1, Lyalya Musina2, Elmira Akhmadullina1, Victoria Morozova1 
...d the homeostasis of the laboratory animals. It positively affected their skeletal and heart muscles, lung tissue, liver and kidneys, where immunity and blood cells develop. Although the swimming test at the beginning of the experiment showed that mice and hamsters had approximately the same indicators of physical endurance, by the end of the experiment, the swimming time of the experimental group animals was significantly longer. The animals receiving pantocr...

Nzube Thaddeus Egboka1*, Olajire Fagbola2, Ugochukwu Nnamdi Nkwopara1, Nnaemeka Henry Okoli1, Akaninyene Isaiah Afangide1 and Tochukwu Victor Nwosu3

Honnakerappa S. Ballari1 and Shashikant S. Udikeri2*

...stance with respect to a laboratory susceptible strain. Similarly, there was 24.95 folds of resistance to deltamethrin in same population with LC 50 128.47 ppm. Among organophosphates a high level of resistance was recorded against monochrotophos with LC50 160.94 ppm and 18.23 resistance ratio in Rachur population itself. The same population exhibited 9.24 resistance ratio towards thiodicarb a carbamate insecticide. Kalaburagi population indicated resistance c...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

... transferability between laboratories and low genotyping error, single nucleotide polymorphism (SNPs) are considered to be preferable molecular markers for genetic population analysis. SNP markers that are suitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ayesha Latif2, Majid Hamid Bajwa1, Iftikhar Ahmad1, Ali Zohaib1, Naeem Faisal1 and Muhammad Hamza3

...y coping the issues like labor scarcity, less plant population, delay in transplanting with more aged nursery, improper fixation of nursery plants in the soil etc. The study results projected mechanically transplanting area (ha) for riding type MT as well as for walk after type MT respectively as 64.78 and 28 annually. Accordingly, there would be 36.15 (000 ha) mechanically transplanted area (5.41%) of province Punjab, Pakistan annually after completion of the...

Momotaz Khanom*, Sheikh Mustafizur Rahman, Muhammad Yousuf Ali, Md. Shahin Parvez, Al-Hasan Antu, Md. Nazmul Ahsan 

...ossilis larvae reared in laboratory conditions. Three-day-old larvae hatched through induced breeding (2.0±0.00016 mg; 0.57±0.09cm) were subjected to four feeding treatments in triplicates (100 larvae/aquarium). The catfish larvae were fed with live Tubifex (LT), dry Tubifex (DT), formulated feed (FF), and commercial feed (CF) two times a day for 60 days. Results showed that larvae fed with formulated feed (FF) grew significantly faster than the ...

Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2

...citrus host fruits under laboratory conditions, and hence are recommended to be further evaluated under field conditions and to consider their potential incorporation in IPM programs against fruit fly infestations on citrus crop.
...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
...s. This international collaborative research was conducted to investigate the prevalence of Aeromonas spp., particularly A. hydrophila in three crustaceans (crab, lobster, and shrimp) retailed in Zagazig, Egypt, and Al-Ahsa, Saudi Arabia. Detection of virulence associated genes in the recovered A. hydrophila isolates was additionally screened using PCR. Furthermore, antimicrobial resistance profiling was examined among the recovered A. hydrophila isolates. The...

Ghulam Murtaza1,6*, Muhammad Irfan Fareed2, Noshaba Munawar3, Muhammad Nadeem Khan4, Gul-E- Zahra5, Taskeen Ahmad6, Muhammad Daud6 and Taqi Raza6

...i larval mortality under laboratory conditions. Result indicated that Spinosad (77.32% mortality) and pyriproxyfen (66.28% mortality) are more toxic to A. aegypti larvae as compared to matrine, chlorfenapyr and imidacloprid after 72-h exposure. While minimum mortality was recorded after 24-h exposure of insecticides in all treatments. So, it can be concluded that spinosad was more toxic to kill A. aegypti larvae.

...

Noor Muhammad Khan1,2, Tariq Mahmood Khalil1,3*, Rashid Rehan2 and Iftikhar Zeb4

...th on-site (in-situ) and laboratory conditions (ex-situ) while A.d was applied only in an ex-situ setup. For T.l, the average uptake of copper (Cu) was 5.40 and 6.46 mg kg-1, similarly for the lead (Pb) average uptake was 4.55 and 4.55mg kg-1, while for nickel (Ni) the average uptake was, 2.80 and 2.76 mg kg-1 in ex-situ and in-situ plantation respectively. Whereas for A.d. the HM uptake was 3.22, 2.27, and 1.49 mg kg-1for Cu, Pb, and Ni, respectively in ex-si...

Mahreen Hanif*, Shafqat Saeed, Mudssar Ali, Muqadas Younas, Huda Bilal, Syeda Fatima Bukhari

...ys interval. A series of laboratory tests were observed over three months to determine the penetration of T. castaneum in different packages. Results showed that the highest population of T. castaneum (larvae and adult) as well as greater weight loss (%) in chickpea was recorded in polypropylene (green) packet in contrast to polyethylene (gauge 2) and china lamination. Significant differences were observed in the overall adult population of T. castaneum (p<...

Nawal Abd-elhafez Hassanain1, Raafat Mohamed Shaapan1*, Ahmed Maher1, Ahmed Badwi Yousif2, Gomaa Desoky Emam2 

...ss (AFI) in our clinical laboratories must rule out any cases of malaria that have symptoms that are similar to the symptoms of the disease and improve patient management by lowering treatment costs and removing other risks related with antibiotic misuse.

Keywords | Cross reactivity, Febrile, Malaria, Brucellosis, Typhoid fever  

...

Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7

... at – 18°C for laboratory analysis. Commercially available ELISA kits were used to measure serum progesterone and estradiol levels. Progesterone and estradiol levels were found significantly high (P<0.05) with the value of 3.46±0.25 ng/ml at day 14th and 4.45±0.34 ng/ml at day 21st in G2 (Pregnant animals), while in non-pregnant group serum P4 concentration was recorded to be 1.05±0.29 ng/ml which is considered as basal level ...

Arfan Ahmed Gilal1*, Lubna Bashir Rajput1, Muhammad Ibrahim Kubar1, Ghulam Murtaza Kaleri2, Tanzeela-ul-Zahra1,3, Muhammad Ishaque Mastoi4 and Zeeshan Rasheed1

...FAW were conducted under laboratory conditions on maize leaves and stems. Three cohorts comprised of 131, 112 and 105 eggs of similar age were used for both leaves and stems. The results indicated that maximum mortality of FAW in both maize stems and leaves were recorded in pupa and 1st larval instar, respectively whereas, maximum survival was recorded in 4th and 5th larval instars. Almost, similar male to female sex ratio was recorded in leaves and stems with...

Alsi Dara Paryuni1, Soedarmanto Indarjulianto2, Tri Untari3 and Sitarina Widyarini4*

...ed on clinical signs and laboratory identification of fungi. Skin scrapes samples were cultured onto Sabouraud’s dextrose agar medium. Hair and skin scraping were conducted at the day-1 (pre-treated with ketoconazole). Subsequently, infected cats were treated with topical application of 2% ketoconazole cream, twice a day for 21 days. Results showed that all of cats positive infected with Microsporum canis. Feature of the skin of cats infected by Microspo...

Najmul Saqib* and Himayathullah Khan

...old head, number of farm labor and adopted agroforestry practices on farm income. It was found that the effect on annual farm income by a unit increase in area under agroforestry was Rs.44.379, number of fruit trees was Rs.0.344 and wood trees on farm was Rs. 0.240, quantity of crops under these systems was Rs. 39.174 and education was Rs. 8.987 which were positive and statistically significant. It was found that intercropping practice in comparison to boundar...

Abdul Hafeez Mastoi1, Muhammad Rashid Nizamani2, Shafique Ahmed Memon3, Jun Jiang1, Xiang-Shun Hu1* and Tong-Xian Liu1*

...um L., was determined in laboratory testing. Of the 5 pea cultivars and 5 temperature levels included in our study, the highest values of the population parameters of net reproductive rate, intrinsic rate increase, finite rate increase, generation time, and fecundity of A. pisum was observed at 27°C, regardless of pea cultivar. In decreased as temperature increased at and above 30°C for each cultivar. Furthermore, differences among cultivars were often...
C.A. Angel-Sahagún1, J.E. Ortega Palomares1, A.A. Hernández-Rangel1, C. Cruz-Vázquez2, R. Montesinos-Matias3, M. Valencia-Posadas1 and A.M. Cruz-Avalos4*
...nis, the dog flea, under laboratory conditions. Nine, monosporic cultures were isolated. Each were pathogenic when exposed by immersion at a concentration of 1x108 conidia/ml, causing between 10-100% mycosis at ten days post-inoculation. Four isolates identified as Bb9, Bb6, Ma9 and Ma10, were the most pathogenic as mycosis reached 100%, which places them as potential candidates to be used as biological control agents.

...

Harpreet Kaur* and S.S. Hundal

...l) of imidacloprid under laboratory conditions. The activity of AChE and GST have been reported as potential biomarkers to assess toxicity levels, hence the activity of these enzymes were assessed to study imidacloprid toxicity. Inhibition of AChE activity was observed in both the earthworm species in all the doses of imidacloprid, indicating neurotoxicity. There was an initial increase in the GST activity followed by its decrease with the duration of exposure...

Jawaid A. Khokhar*, T. J. Ursani, Fareeda Memon, Samina Malik, Asif Soomro and Fareeda Shah

...at the advanced research laboratory of Arachnology and Entomology. The collected samples were kept at an Average temperature and humidity between 28.2 ± 0.47 to 38.78 ± 0.47 0c, 57.6 ± 0.55 to 72.6%, respectively.

...

Khalid Javed Iqbal1, Irfan Baboo2*, Arshad Javid3, Noor Khan4, Hamid Majeed5, Azra Anwer1, Zahid Farooq2, Muhammad Altaf6 and Moazam Jalees7

...erred immediately to the laboratory for the assessment of their blood profile. There was recorded no mortality after 96h of exposure period. IONPs significantly affected the counts of WBCs, Hb, MCH and MCHC of experimental and control groups. The analysis of blood profile demonstrated that haematological parameters; the number of WBCs and Hb were increased in T1 and decreased in T2 in relation to T0. The counts of RBCs, HCT, MCV and PLT were found to be increa...

Tooba Ather1, Muhammad Rafiq Shahid2*, Muhammad Ahsin Ayub3, Javed Iqbal2, Muhammad Asim Bhutta2, Muhammad Akram2, Naveeda Anjum4, Muhammad Rizwan6, Hafeezur Rehman5 and Umar Farooq5

Amina Batool1*, Saba Aleem1, Ali Nawaz1, Muhammad Imran Khan1, Waheed Arshad1, Muhammad Aslam2, Shiraz Ali3 and Muhammad Zeeshan3

...Rabi 2019-20; one at the laboratory by growing three diverse genotypes of wheat at four different levels of seeding densities in pots and the other at field area of Barani Agricultural Research Station, Fateh Jang at four discrete levels of seeding density of 80, 100, 120 and 140 kg ha-1, respectively. The experiment was laid down using a RCBD with three replication and four treatments. Different seed rates and genotypes significantly affected all plant traits...

Shambel Boki Dabi

...ite of three streams for laboratory study. Water quality parameters such as CO2, Chloride, and alkalinity were instantly assessed using the titration method, while water turbidity, temperature, water pH, and dissolved oxygen were measured directly on site. A simple test kit was used to evaluate nitrite and ammonia levels. The result revealed a high level of alkalinity (66.3±39. 1mg. L-1) at downstream and lowest concentration (58±40mg.L-1) was fo...

Abid Ali1*, Safia Naureen Malik3, Muhammad Akmal2, Hafeez Ullah Rafa2 and Abid Subhani3

Muhammad Jalees
... moderating effect of collaborative advertising on its association. The survey-based methodology was used for the collection of data from the Telecom industry in Islamabad and Rawalpindi. 250 valid questionnaires was collected from different telecom industries. Data were analyzed using the Cronbach alpha reliability technique with the help of Statistical Package for Social Sciences (SPSS) version 23.0. Regression has been conducted to test the hypothesis. The ...

Sharon Zulfiqar1, Mubashar Hussain1*, Kiran Aftab1, Muhammad Faheem Malik1, Rizwan Khursheed2, Somia Liaqat1 and Maimoona Kanwal1

Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3 

...sts. Complementing these laboratory tests with methods for assessing the expression of interferon (IFN) genes can expand the possibilities of diagnosing animal cancers and improve the accuracy of differential diagnosis of benign and malignant neoplasms, in particular of the mammary gland (MG). Methods. Surgical material obtained after unilateral mastectomy with simultaneous hysterovariectomy, as well as peripheral blood samples of cats with MG tumours was used...

Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3* 

...ocyte differentiation by laboratory analysis. Descriptive morphological data and quantitative data were analyzed using ANOVA testing. The adult cuscus appeared to have more spots (the male spotted pattern was larger than that of the female) than the juvenile cuscus, where the body pattern had not formed spots (spread). Based on such an obvious spot pattern, the sexes of S. papuensis could be distinguished by males having larger spots than females. The S. papue...

Shahid Ali1*, Murtaza2, Waqas Ahmad1, Nargis Bibi3, Aftab Khan2 and Jahangir Khan

...060, 0.246 and 0.098 for labor hours, tractor hours, chemicals, urea and irrigation numbers respectively. Results of inefficiency’s model exposed that education and farming experience had a significant and adverse influence on technical inefficiency, age had a positive significant effect on farmers’ technical inefficiency whereas tenancy status and primary source of income were found insignificant. The mean technical efficiency of the sampled farme...

Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari

...research. Phase 1 of the laboratory experiment determined the composition of the concentrate and tofu dregs media on the nutrient content of Tenebrio molitor. This study method was a completely randomized design (CRD) with six treatments and three replications. The treatment variations consist of commercial ration (CR) and tofu dregs (TD) were treatment P1 (100% CR), P2 (80% CR + 20% TD), P3 (60% CR+ 40% TD), P4 (40% CR + 20% TD), P5 (20% CR + 80% TD) and P6 (...

Hassan Raza1, Muhammad Zeeshan Majeed1*, Muhammad Irfan Majeed2, Mujeeb-Ur-Rehman1 and Muhammad Asam Riaz1

...e formulated beads under laboratory and field conditions.

...

Veasna Chem1 †, Hong-Seok Mun1,2 †, Keiven Mark B. Ampode1, Shad Mahfuz1, Il-Byung Chung1, Muhammad Ammar Dilawar1,3, Chul-Ju Yang1,3,* 

...s the greatest impact on labor rate and little size. Digital infrared thermal imaging (DITI) technology can easily recognize and detect animals’ temperature changes, which would be useful in farm animal production. This study investigates the possibility of replacing a contact thermometer with a non-contact thermometer and digital infrared thermal imaging camera (DITI) to detect the estrus of gilts. Seven gilts (129.21±2.51kg) were considered for ...

Arshad Mahmood Malik1*, Nigah Hussain1 and Nasim Akhter2

...8, addresses agriculture labor on the lines of industrial labor to improve its supply. This study is designed to ascertain the factors effecting agricultural labour supply of irrigated area of Khushab District of Punjab. Primary data of three villages was collected having different cropping patterns and irrigation systems of the study area. Multivariate model was developed to ascertain the various socio-economic factors i.e....

Muhammad Rabiul Hasan1, Zannatul Mawa1, Muhammad Ashekur Rahman1, Selina Yeasmin2, Yahia Mahmud2 and Muhammad Yeamin Hossain1*

...sBazar) with the help of laborer in the presence of Principal investigator and research assistant. This study also revealed the maximum recorded length of A. granosa and first report of M. lamarckii from the worldwide water bodies. We observed maximum length of A. granosa was 7.50 cm (maximum recorded length) and 6.83 cm for M. lamarckii. Allometric coefficient (b) value was 2.61 and 2.83 for A. granosa and M. lamarckii, respectively that indicating the growth...

Olufemi Bolarin, Sijuade Adebukola Adebayo and Sola Emmanuel Komolafe*

...ds that government in collaboration with development agencies should intensify effort in creating awareness to farmers on the several ways of building resilience to mitigate the menace of negative influence of climate change.
...

Sajjad Khan, Fahad Karim Awan*, Zulfiqar Ali Gurmani, Muhammad Usama Hameed, Allah Bakhsh and Kainat Bibi

...conducted at NARC fodder laboratory to study the response of two oat cultivars namely NARC and PARC Oat against drought and salinity stress. Salt concentration of 80 mmolLˉ¹ showed non-significant effect on germination % and shoot length of both oat cultivars except salt concentration of 120 mmolLˉ¹, but tolerance in NARC, Oat is higher than PARC oat. Salt concentrations positively increased root length, except in PARC oat at 120 mmolLˉ¹. Dro...

Farheen Shaikh*, Saima Naz and Nadir Ali Birmani

...t to the Parasitological laboratory of Department of Zoology, University of Sindh, Jamshoro. The study was carried out from April 2015 –March 2016. Guinea fowls Numida meleagris (Linnaeus, 1758) were sprayed with Permathrin (Coopex powder) and ket on white paper sheets for about 32-35 minutes. The fowls were checked time to time after 2 to 3 weeks. The chewing lice were collected hand picking methods with fine brushes and preserved in 70-75% ethyl alcoho...

Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3

...d mesophilic microbes collaborate to digest solid agricultural waste under controlled circumstances, resulting in a stable form of organic matter. Earthworms produce vermicompost, which contains growth-regulating hormones, macro-micro nutrients, plant-immobilized microflora, growth regulators and promoters and cellular portion degrading enzymes (cellulose, proteases, lipase, amylases, chitinase), which degrade organic substrates even after they have been secre...

Kanwal Hanif1, Dilbar Hussain1, Qurban Ali1, Asad Aslam1*, Tamsila Nazir1, Muhammad Saleem1, Muhammad Faheem Akhtar1, Muhammad Zubair2, Najuf Awais Anjum3 and Muhammad Umar Qasim1

...conditions at toxicology laboratory Entomological Research Institute Ayub Agriculture Research Institute, Faisalabad. The viability of eggs was evaluated by exposing egg cards to insecticides by dipping them in insecticide solution for 10 seconds while adult survival was investigated through dipped surface residue bioassay method. The results revealed that new chemistry insecticides viz., Flubendiamide, Flonicamid and Chlorantraniliprole were comparatively saf...

Yêyinou Laura Estelle Loko1*, Joelle Toffa1, Azize Orobiyi1, Gbèblonoudo Anicet Dassou2, Rolande Okpeicha1, Dieudonné Gavoedo1 and Alexandre Dansi2

...us were evaluated in the laboratory. The correlations and contributions of the studied traits were evaluated using correlation path coefficient analysis. The tested soybean varieties showed a variation in physical seed characteristics. A differential susceptibility of soybean varieties to C. maculatus was observed, with the white seeded variety Whéwhé having longest and largest grains was the most susceptible. Based on the Dobie susceptibility in...

Dong-Min Hou, Hong-bi Peng, Wan-Long Zhu* and Zheng-Kun Wang*

...male T. belangeri in two laboratory experiments. Our footage provides the first video documentation of infanticide, cannibalism, and parental care behavior in T. belangeri. Analysis of the video data indicated that the observations were related to nutrition hypothesis, hormone level hypothesis and stress hypothesis. We also suggest that the infanticidal and cannibalistic behaviors of tree shrews might be related to their dietary habits. Although reactions to i...

Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4

...s, including facilities, labor, experience, and budget. PGF2α induces luteal regression followed by estrus and ovulation in cows when administered during the luteal period. Progestin suppresses the activity of the ovary and inhibits the dominant follicle maturation due to the suppression of both FSH and LH. Gonadotropin-releasing hormone or an analog is administered with PGF2α to estrous- cyclic and noncyclic cattle disrupts the patterns of follicu...

Abdul Latif1, Shahid Sattar1, Fazal Maula2, Imtiaz Khan4*, Asim Iqbal3 and Said Hussain Shah1*

...ity was 6.58 days. Under laboratory conditions, the highest 100% mortality was caused by lamda-cyhalothrin and cypermethrin after 72hrs followed by A. indica seed extract (81.00) and P. hydropiper (78.80) when tested under laboratory conditions. Based on the current results, it is concluded that lamda-cyhalothrin and A. indica caused high mortality under lab and field conditions, which can be included in future IPM programs....

Ekechukwu Esther1, Ohanu Chinenye1, Ossai Nelson1*, Elijah Okwuonu1, Hinmikaiye Funmilayo1, Ngene Innocent1, Andong Felix1, Ikegbunam Clara2, Echude Daniel1, Ekeh Felicia1 and Odo Gregory1

...ys department of zoology laboratory of University of Nigeria, Nsukka. The rats were divided randomly into six groups of 0%, 10%, 20%, 30%, 40% and 50% C. cajan diet respectively, with a total of 9 giant rats in each group. The diets contained protein, fibre, ash, fat and sugar /energy. Feed efficiency increased in the rats fed on 30% and 50% C. cajan. Crude protein digestibility decreased (P< 0.05) with increased levels of C. cajan. The values of plasma tot...

Syeda Batool Zehra1, Abdullah G. Arijo2, Aly Khan3, Nasira Khatoon1* and Samina Waheed1

... were collected from the laboratory of Jinnah Hospital, Karachi, Sindh, Pakistan. The data of 60 pinworm-infected children were recorded. These children were of various sexes and ages. Pinworm prevalence in children by gender from 2019 to 2020 demonstrates that females have a higher rate of incidence than males because of unsanitary circumstances and hormone production. Traditional pinworm diagnosis via PCR for pinworm detection has been performed. The finding...

Murad Ali Rahat1*, Muhammad Israr2, Hakim Khan1, Ishtiaq Hassan1, Mohammad Islam1 and Muzafar Shah3

...n in accredited forensic laboratories, predicting blue and brown human eye color from DNA samples, hence, may be a useful tool in forensic investigation.

...

Akbar Hayat1*, Ehsan Ul Haque1, Marayam Nasir2, Rab Nawaz3, Tariq Mahmood4 and Sohaib Afzaal1

...ext-align: justify;">An elaborated study on evaluating quality characteristics of exotic fruits belonging to orange group of citrus was carried out at Citrus Research Institute Sargodha. The candidate varieties/cultivars were Rhode Red Valencia, Cara Cara, Amber Sweet, Sun Star and Salustiana. All of these different five citrus varieties evaluated objectively assessing their suitability both for direct consumption and value addition. The work spread over two y...

Zarnosh Habib1, Muhammad Ibrahim2*, Nasir Shah2, Israr Ullah3 and Norman Javed Gill4

...(24, 48, 72 and 96 h) in laboratory at 25 ± 2ºC, 60 ± 5% relative humidity and a photoperiod of DL 14:10 h. The observations indicated a significant effect of different depths of host pupae and its age on the amount of parasitism by parasitoid. Maximum significant (F = 10.78, P < 0.0001) parasitized pupae were recorded at 72 h of age at surface of the debris, while minimum number of parasitized pupae were recorded in a day old pupae at 3 ...

Layth Saleem Salman Al-Shihmani

...of each horizon, and the laboratory examination was conducted for the physical and chemical properties. As for the mineral examination, it was conducted for only two depths (surface depth and subsurface depth). The results showed a variation in the chemical properties represented by the electrical conductivity values, pH of soil, organic matter, carbonate minerals, and the cationic exchange capacity of the soil between the front and rear of the Kut Dam. As for...

Nosheen Jehajo*, Nasreen Memon, Mansoor Ali Shah and Naheed Shah

...e the economic losses. A laboratory experiment was conducted in August-November, 2019. The experiment was laid out at (30±2 ºC and 65±5% relative humidity), to investigate the insecticidal efficacy of Azadirachta indica (A. Juss) (neem) and Ricinus cummunis (L.) (castor) seeds oil that effect the various biological parameters of C. maculatus fed on Vigna radiata (mung beans) including mortality, oviposition, and adult emergence using three r...

Arif Mehmood1*, Muhammad Naeem2, Abu Bakar Muhammad Raza1, Muhammad Asam Riaz1, Muhammad Zeeshan Majeed1, Nadra Khan3 and Waqas Raza4

Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
...utosomal STR loci in our laboratory for validation purpose and routine use. In this regard, swab samples from different regions of Turkey (n=350) were collected and studied. The validation of the GlobalFilerTM Amplification Kit was carried out in accordance with the guidelines published by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our study was conducted to show the efficacy and performance of the kit for sensitivity, specificit...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

...ng other microbiological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and Indole Tests). A high level of total plate count (TPC) and E. coli was observed from both raw and pasteurized milk. The results also indicated a high incidence of ETEC i.e. 63.63% and 50% in raw and pasteurized milk, respectively. The most occurring enterotoxins are ST-I and ST-II (40%) followed by LT-II and ST-II, 20% each, followed by ST-I...

Sumaria Maqsood1*, Muhammad Ali 2, Amna Shoaib3, Shahbaz Ahmad2 and Noor-ul-Ain2

... for six generations for laboratory and field experimentation.

...

Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3* 

...emiology relay on the collaboration between biostatistics and epidemiology to provide a basis for the veterinary field and clinical care. Since discriminant analysis as a parametric test is more powerful than its non-parametric alternatives, we aimed to evaluate the performance of the single discriminant analysis in classification between Baladi buffaloes and crossbred using clinical epidemiological measures of some productive and reproductive traits such as m...
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
... banana was evaluated in laboratory conditions. The olfactory results of the present study showed that all botanical extracts exhibited the repellent effect against B. zonata, but the methanolic extract of cinnamon showed the highest repellence activity against B. zonata. Moreover, no side effects were observed on the post-harvest quality of banana treated to different methanolic extracts as compared to control. This shows that botanical extracts could be used...

Adrial Adrial1*, Rudy Priyanto2, Salundik Salundik2, Ahmad Yani2, Luki Abdullah3 

...tion method supported by laboratory analysis. Reproductive performance data were obtained through direct interviews and recording cards. Soil and forage samples were taken from the main forage source locations. A total of 138 blood samples were taken through the jugular vein from Bali cows and their crossbred cows with different physiological status. Soil and blood serum samples were analyzed in laboratory using Atomic Absor...

S. F. A. Awad-Allah 1 and M. S. Khalil 2

... fenamiphos 40% EC under laboratory and field conditions. Experiments were carried-out during two successive seasons (2016 and 2017). Results of the laboratory tests showed that the toxicity of VCT and Nemaless® to M. incognita second-stage juveniles (J2) was increased as their concentrations and exposure times were increased. The LC50 of VCT ranged from 30.86 × 103 to 83.77 × 105 mg/l, while the LC50 of Nema...

R. Hadadfar1, E. Mahdikhani-Moghadam2†, S. Baghaee2 and M. S. Bajestani1

...re transferred on ice to laboratory and nematodes were extracted by centrifugal methods. Psilenchus species were identified on morphological and morphometrical characters based on recent valid keys. Seven species from Psilenchus genus were identified viz., Psilenchus curcumerus, P. aestuarius, P. iranicus, P. hilarus, P. bilineatus, P. pratensis and P. bahiablancae of which P. bilineatus, P. pratensis and P. bahiablancae are new reports of these species for Ir...
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...oidogyne incognita under laboratory conditions. All concentrations significantly inhibited egg
hatching (31.24–66.35%) and mortality of second stage juvenile. Inhibition of egg hatching (%) and mortality (%) of
J2 were increased linearly with increasing concentration of EOs. Corymbia citriodora EO was more effective than
E. camaldulensis in inhibiting egg hatchability and suppressing J2 viability. Probit analysis resul...

Ahmed S.M.H. EL Roby

...arococcus sacchari under laboratory conditions (27 ± 2 °C,
RH 65 ± 10%). The EPNs used in the study are Steinernema sp. (strain: AT4), Steinernema sp. (strain: EMB),
Heterorhabditis bacteriophora (strain: EKB20), Heterorhabditis sp. (strain: EIK) and Steinernema glasri (strain:
New Jersey). The bioassays were carried out on Petri dishes containing parts of pumpkin infested with adult females
of ...

I. Kepenekci1†, S. Toksöz2, T. Atay1 and I. Saruhan2

...nidae: Scolytinae) under laboratory conditions. The studies were conducted in June, 2017 at
the Ondokuz Mayıs University (Samsun, Turkey). The suspensions of nematodes were applied at one concentration
(1000 IJs ml-1) (200 IJs insect-1 or approximately 15 IJs cm-2) at 25°C temperatures. The data for mortality was
recorded after 2, 4, 6 and 8 days intervals. Insect mortality of the adult was higher in all nematode treatm...

K. Taimoor1† and F. Shahina2

...nita both
in laboratory and pot experiments at doses of 0.5g/lit water (0.5% w/v), 1.0g/lit water (1% w/v), 1.5g/lit water (1.5%
w/v), 2g/lit water (2% w/v), 2.5g/lit water (2.5% w/v) and 3.0g/lit water (3% w/v). Zatoria multiflora, Achillea
santolina, Ferula oopoda and Nepeta cateria showed more efficacies as compared to other medicinal and aromatic
plants both in laboratory a...

F. Shahina† and G. Mehreen

...p; abbasi (AY944002). In laboratory experiment, biological life cycle of three isolates of S.  abbasi
PAK.P.S.9 (EF469773), PAK.S.S.15 (JN571086) and PAK.S.S.16 (JN571096) and one other isolate of
Heterorhabditis  indica PAK.S.H.56 (GU130179) were applied against fungus growing termite Microtermes 
mycophagus  (D.). The study were also influenced by mortality; days to death, infection cycle length and
A.A. Tanimola and B. Fawole1
...e="text-align: justify;">laboratory. Data were collected on inhibition of egg-hatching, mortality of juveniles and analyzed using ANOVA (P
 0.05). Both concentrations of Aloe species extracts inhibited egg-hatching and second-stage juveniles mortality
was observed significantly when compared with water control. The AE of AKY leaves at 50,000 mg/kg was the
most effective in egg-hatching inhibited (95.4±1.7%), followed...

M.A.M. El-Saedy, A.A. Mokbel*† and S.E. Hammad**

...ting tomato plants under laboratory,
greenhouse and field conditions. Treatments with essential oils of arugula, camphor, castor, garlic, nigella, onion
and sesame resulted in 48.0-92.7% reduction in numbers of root galls/root under laboratory conditions. Similarly,
application of these oils resulted in great reductions of 59.2-92.8% and 47.4-89.6% in numbers of root galls and

I. Umar† and A. Mamman*

...anica in the
laboratory and screen house was investigated. Nematodes egg suspension at 100 eggs/5ml syringe introduced into
each Petri dish containing both crude and diluted extracts of fruit powder (5, 10, 15 and 20 ml) except the control,
which received only distilled water. Petri dishes were treated to the same concentrations of the extract in Petri
dishes containing 1000 second stage juveniles of M. javanica....

O. A. Fabiyi

...>
evaluated in the laboratory using a 4x4x3 factorial experiment, while the crude extracts were tested on
Meloidogyne incognita infecting Abelmoschus esculentus in the field. Three hundred juveniles were used for the
assessment. Acetone extract coded DNLO/Me2CO caused a significant increase in plant height, number of leaves,
number of fruits per plant and fruit weight per plant as compared with Carbofuran (p<0.05). ...

T.S. Rizvi and F. Shahina†

...) at 28 ± 2 C in laboratory. Gas chromatograph interfaced to a Mass
spectrometer (GC-MS) results indicated that C. colocynthis contains various bioactive compounds. The mentholic
extracts of seed had the highest mortality (100%) at highest exposure time (72 hrs) while, the pulp and fruit extracts
showed 60 and 80% mortality, respectively. Among various extracts of creeper hexane extract (HH-CC) showed
100...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk 

...bsp;

...

Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4

...eir metabolites. In this laboratory study, two bacterial isolates i.e. Xenorhabdus spp. associated with EPN Steinernema glaseri (Steiner) (Steinernematidae) and Photorhabdus spp. associated with EPN Heterorhabditis bacteriophora (Poinar) (Heterorhabditidae), were evaluated against armyworm Spodoptera litura Fabricius (Lepidoptera: Noctuidae) which is a destructive lepidopterous pest. These EPNs associated bacterial isolates were applied @ 4×107 CFUs/mL a...

Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2

...ment of mother stock and labor intensive method. To address these issues an alternative technique has been employed for quick multiplication of litchi. In the present study we have investigated the influence of various levels of Indole Butyric Acid (IBA) on four Litchi cultivars (Gola, Surahi, China and Bedana) for root induction, its development and success rate of survival in hardwood cuttings. Six treatments of IBA (1000, 2000, 3000, 4000, 5000, 6000ppm) an...

Laveena Shekhawat, Sheethal S* 

...icrobiology sections for laboratory diagnosis. Identification of sickle shaped hooklets on wet mount and ZN staining confirmed the diagnosis. The patient received post-operative oral albendazole which he failed to start immediately leading to recurrence of the parasitic hepatic cyst. Conclusion: Treatment is a combination of surgery and post-op anti-parasitic drugs. Delay in initiating albendazole therapy can lead to recurrence of the cysts in the same sites.<...

Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2, Muhammad Shahroaz Khan1 and Muhammad Zeeshan Majeed1*

...arvae of S. litura under laboratory and then under the field conditions. In both trials, insecticidal treatments affected significantly the mean mortality or reduction of S. litura larvae recorded both at two and five days post-treatment. In laboratory bioassay, combinations of flubendiamide and A. indica, flubendiamide and N. tabacum and of spinetoram and A. indica formulations caused significantly high mortality (100%), fo...

S.H.M. Faruk Siddiki1*, Md. Golam Morshed2, Md Robiul Karim1, Lutfun Naher1, Md. Sodrul Islam3

...ll as other conventional laboratory procedures. The recorded clinical cases were classified into ten major diagnostic groups. Parasitic diseases (30.16%), infectious diseases (21.84%), general systemic states (20.12%), digestive disorders (18.55%), gyneco-obstetrical diseases (4.37%), and surgical cases (3.06%) were recorded as major clinical problems, with other diseases having a prevalence of less than 1% for each. Among the ten categories, the prevalence of...

A. Yu. Aliev1*, A.A., Aliev1, A.M. Musaev1, M.Z. Magomedov2 

... of mastitis in ewes. In laboratory studies using the Al-test diagnosticum, the main criterion for the presence of subclinical mastitis in animals was the formation of a well-formed purple jelly-like clot (moderate to dense) of in the milk samples, which was partially (+) or entirely (++) of thrown of out the from the hole in plate with stirring. On the plate, with a negative reaction (-), the mixture of milk and the indicator remained homogeneous, pale ...

Ayaz Ahmed1*, Muhammad Zulfiqar1 and Saadutullah Khan2

...emittances and fifth was labor work respectively. Study results discovered that 69% of sample households had earned income from the sale of NTFPs above mean income (60458 Pakistani rupees) per season while 31% of sample households had earned income from the sale of NTFPs below mean income (60458 Pakistani rupees) per season. Sample households reported that during emergencies, only income earned from non-timber forest products stabilized their economy. Sample h...

Saba Iqbal1, Salman Khurshid1*, Hafiza Mehwish Iqbal1, Qurrat-Ul-Ain Akbar1, Aqeel Ahmed Siddique3, Saqib Arif1, Shahid Yousaf2, Masooma Munir4, Abdul Karim Khan4, Shazia Arif5,  Abdul Ahad6 and Muhammad Arif7

...l method in the field of laboratory instrumentation. The attempts have been made to explore the utility of NIR for variety of samples matrices. The present work has been undertaken to assess the efficacy of NIRS method for quantitative characterization of wheat grains in terms of hardness. Since wheat is the staple food and its hardness is one of the basic quality traits being used in local and international trade. In addition to the NIR-spectroscopy, wheat gr...

Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5

...pared and tested against laboratory-reared adults and pupae of C. pipiens. The safety results showed that the ordinary form of cinnamon oil was safer for human skin cell fibroblast and human epidermoid Skin carcinoma than the other forms at high concentrations. Meanwhile, CNE was potent antibacterial activity even at the lowest concentrations. Moreover, CNE showed better pupicidal activity than the other forms with LC50 at 322 ug/mL lower than pure cinnamon oi...

Muhammad Shahid1*, Muhammad Idrees1, Afza Rasul2, Iram Amin1 and Samia Afzal1

...ues were assessed in the laboratory. Receiver operating characteristics were used to find the biomarkers of infection. Logistic regression analysis revealed that liver enzymes (ALT, AST, ALP, γGT, albumin), blood cell count (globulin, platelets, MCHC RBC, WBC, monocytes% and lymphocytes%), lipid profile (HDL, LDL, VLDL, TC) were significant predictive factors in HCV infection. Similarly, ROC analysis suggested that ALT, AST, ALP, MCHC, TC, and VLDL varia...

Muhammad Sohail1* , Zubair Ali1, Hamidullah1, Yasir Amin1, Mehwish Malik1, Said Sajjad Ali Shah2 

... 579 Acres of land in collaboration with Australian government. Most of the area of the LES comprised of hilly terrain, where 1195 mm rainfall observed throughout the year. The animals of the LES Jaba are sent for grazing on the pasture with naturally grown at evening stall feeding is also practiced. It was observed from the data obtained that in sheep mixed infections were very common (30.4%) followed by Strongylus (21.6%), Eimeria spps. (15.5%) and Haemonchu...

Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5 

... immediately sent to the laboratory for processing. Parameters measured were sperm motility, abnormality, viability, acrosome integrity, and membrane integrity. Liquid chromatography-mass spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled b...

Shabana Memon1*, Aamir Ali Abro1, Muhammad Iqbal Jakhro2, Aqsa Farid3, Maliha Habib4, Maqbool Ahmed5, Liaquat Ali Bhutto6, Saba Ambreen Memon7 and Muhammad Farooq8*

...stically decreased under laboratory conditions due to osmotic stress. The genotype SDW-3 showed the greatest decrease under increased osmotic stress brought on by PEG-6000 (-5.0 MPa). As a result, the genotypes AST-1(V1), SDW-1, and SDW-2 may be employed in future breeding programmes and are drought resistant.

...

Saleem Hussain1, Muhammad Tayyab1, Tauqir Anwar1, Talha Nazir2*, Muhammad Zeeshan Majeed3, Zohaib Asad2,4, Muhammad Adnan3 and Tajwar Alam5

... graminum Rondani) under laboratory and field conditions. Using foliar spray method, toxicity bioassay was conducted with three different concentrations (i.e. 250, 500 and 1000 ppm) of methanolic plant extracts under controlled laboratory conditions, while three different concentrations (25, 50 and 75 g/L) of each botanical extract was tested in the field trial. Results showed a significant mortality and suppression of S. gr...

MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF

... for 30 days at constant laboratory conditions. After 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-lethal concentrations was compared with the control. Dose dependent increase in the activities of peroxidase enzyme was observed in the tissues of fish as compared to the control group. Peroxidase activity in the liver of fish from all t...

ASIMA BANO1, HAFIZ MUHAMMAD TAHIR*2, AROOSA RASHEED3, MUHAMMAD ARSHAD4, SHAFAAT YAR KHAN1 & RABIA YAQOOB5

... from various diagnostic laboratories of Sargodha city. Levels of non-specific esterases were determined in the normal and breast cancer patients. The levels of non-specific esterases were higher in healthy individuals than the breast cancer patients. So, levels of non-specific esterases could be used as a marker for the diagnosis of breast cancer.
 
Key Words: Esterases, breast cancer, risk factors, diagnosis
...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...y short time compared to laborious, non-specific and expensive protocols. ELISA, Nested PCR has been used for many years in different countries. Citrus orchards in Sargodha region were surveyed and leaf samples showing typical symptoms of citrus canker were collected. Infected section of leaf was taken for isolation of bacteria. Lesions were cut into parts and streak bacteria by the help of inoculation loop grown in nutrient medium. Pure bacterial culture of X...
 
 MONIS HUSSAIN SHAH1*, RIAZ UR REHMAN2, RIAZ ALI SHAH1, RIZWAN RAFIQUE3, MUHAMMAD USMAN4, SAJIDA BIBI5 AND SADIA YASEEN
...6 hr. The environment of laboratory during research was 20oC. The best response for extending the freshness was observed in cv. Bonnie (Orange) that performed excellent when placed in medium level of 6-Benzyleadenin (20-40ppm with 10-15g/l of Sucrose). The results of 6-Benzyleadenin (20-40ppm with 10-15g/l of Sucrose)...

Hemayet Hossain1, Md. Masud Parvej1*, Khadiza Akter Brishty3, Muhammad Ali4, Junayed Ahmed4, Asibul Hasan4, Rubel Miah4, Md. Piplu Mia4, Saad Muhammad Rafe-Ush-Shan4, Md. Nazmul Alam4 and Md. Zahidul Islam Khan2

...examination using common laboratory techniques. A total of 28 infectious and non-infectious diseases and disorders in ruminants were detected, whereas the pooled prevalence (PP) of the infectious disease group in cattle was 38.44%, (95% CI: 33-42) and in goats it was 34.43%, (95% CI: 29-39). Among them, FMD was recorded the highest prevalence at 5.78%, (95% CI: 3.8–8.4) and the lowest prevalence at 0.89%, (95% CI: 0.2-2.1) for Tetanus in cattle. The PP o...

Siti Azizah1*, Salsa I Latifah1, Irfan H Djunaidi1, Anif M Wati1, Achadiah Rachmawati1, Siti Hamidah2 

....06%, with an outpouring labor of as much as 78,128 man-days/year and a productivity level of 112,515 IDR per man-day.
 

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Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5

...n and transported to the laboratory of the Department of Environmental Science and Engineering at the Government College University Faisalabad for further analysis. The larval morphological measurements were carried out using a stereomicroscope, which included changes in head length and width, thoracic length and width, abdominal length and width, and total length of the larva. Every month, the fourth instar larvae (n=36) were investigated for body size measur...

A. A- E. Abo-Elkhier1, K. S. E. Abdel-Wahab2, M. A. Ali3, M. A-H. El-Sayed4, N.A-K. Saleh5 and A. A. Atef6

...l full-term pregnancy in labor were included in the present study. Maternal blood was tested for anti-HEV IgG and IgM antibody and the positive cases were tested for HEV Ag and HEV RNA. The fetal tissue from anti-HEV IgG and IgM positive aborted women and cord serum of infant of anti-HEV IgG and IgM positive full term delivered women were tested for REV Ag and HEV RNA. Among 120 aborted women. 2.5 (3/120) of them were positive result with anti-HEV IgM. Anti-HE...

Muhammad Zeeshan Khan1*, Umar Khitab Saddozai1, Abdul Aziz Khakwani1, Mohammad SafdarBaloch1, Atiq Ahmad Alazai2 and Muhammad Amjad Nadim1

...hoeing (hand weeding) if labor is not a major problem, however among the available weedicides in the market which were used in the current research the Pallas (pyroxsulam) weedicide proved to be the excellent for obtaining higher weed control efficiency and grain yield.

...

Shabana Mangi1*, Abdul Manan Shaikh1, Waheed Ali Panhwar1, Javeed Ali Ujjan1, Fakhra Somroo1, Shazia Praveen Solangi2, Sumbul Mureed Mastoi3 and Ranjeet Kumar1

Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3

Faiza Siddique1, Muhammad Shahzad Ahmed1, Rana Arsalan Javaid1, Alvina Hanif1, Maria Rabnawaz1, Muhammad Arshad2, Irum Raza3 and Abid Majeed1*

Nitya Salsabila, Djalal Rosyidi, Agus Susilo* 

...d. The method used was a laboratory experimental method using a completely randomized design (CRD) with 6 treatments and 4 repetitions, namely P0: without the addition of liquid smoke (control), P1: liquid smoke without sonication, P2: sonication liquid smoke for 5 minutes, P3: sonication liquid smoke for 10 minutes, P4: sonication liquid smoke for 15 minutes, and P5: sonication liquid smoke for 20 minutes. The results showed that the addition of sonication li...

Abdul Hassan1*, Arshad Farooq1, Muhammad Ishaq2, Ghulam Sadiq2 and Asif Nawaz3

... difficulties in finding labor and migration of family members. Production strategies factor were weather forecast for adjusting production practices, adopt new technology and production diversity. Leasing inputs, market monitoring and switching to other markets had loadings in market strategy while maintaining good relationship with friends/relatives and maintaining good relationship with market actors in institutional strategy. Human strategy was related to ...

Indira Jurinskaya1*, Tynyshtyk Kenzhebaeva2, Kairat Iskakov3, Bekzat Niyazbekov1 

...ucing the cost of manual labor in the technology for obtaining down. The research was carried out at the “Aiganym” farm of the Zhambyl district on one-year-old goats of the Kazakh coarse-haired and Gorno-Altai downy breeds as well as their crossbreeds. Animal groups were formed according to the principle of analogue groups, taking into account age and live weight. Animals downy breed were more than 2.5 times higher than the animals of the local Kaz...

Winda Sartika1*, Budi Hartono2, Hari Dwi Utami2, Lilik Eka Radiati2 

...d-19 pandemic through collaboration marketing and supply chain management approaches. Research using the survey method, snowball sampling in the rendang industry with raw beef, and purposive and accidental sampling on the supply chain institutions involved in rendang production. The research respondents comprised 61 respondents from the rendang industry, including suppliers of raw materials, distributors, and consumers of rendang with qualitative and quantitat...

Desi Kurniati Agustina1,3, Suyadi Suyadi2*, Veronica Margaretha Ani Nugiartiningsih2, Kuswati Kuswati2 

Muhammad Ali1*, Rukhsana Perveen2, Khalil Ahmed3, Ghulam Raza1, Muhammad Akbar1, Maisoor Ahmed Nafees3 and Muhammad Ayub4

...aca comparatively in the laboratory and under field conditions. The 4th instar larvae were found to be more voracious than adults.

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Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
...as tested using clinical laboratory and standard institute guidelines. On the other hand, resistant strains were put to antibacterial testing assay against metallic nanoparticles both by well diffusion and broth microdilution method. To analyze the data, both probability and non-probability statistical tools were applied using SPSS version 22 of statistical software at 5% probability. The current study showed 24.56% of S. aureus positive from commercial dairy ...

Afifatul H.A. Adilah1, Junun Sartohadi2* and Suci Handayani1

...stic measurements in the laboratory. The data collection process began with a study of the landscape of the research area which was divided into three zones, namely residual, erosion, and deposition. In each zone, coconut and Mahogany plants were selected with the ideal morphology for measurement of interception, throughfall, and stemflow during rain events. Soil sampling under the plant canopy was used to measure texture, bulk density, particle density, poros...

Rusul Adnan Dawood, Hasan F.K. Alghetaa*

...gCl2) with or without collaboration of resveratrol (RES) supplement. Forty-five adult Sprague Dawley, 8-10 weeks old female rats weighing 170-220 g were randomly grouped as following; control group (C) kept without any medication. Dimethyl sulfoxide (DMSO) used as vehicle to prepare RES treatment was given to D-group. RES administered to R-group. Challenge group of rats (HD) was administered HgCl2. The last group of rats (HR) was given HgCl2 with RES in the sa...

Mudawamah Mudawamah1*, Sumartono Sumartono1, G. Ciptadi2, E. Susanto3, Y. Hartoyo4, L. Affandhy5,6

...dy with quantitative and laboratory analysis, with statistical analysis using ANOVA and LSD with SPSS Statistic 9 software. The research design used descriptive with total sample of 60 heads (morphometry and physiological status) and 24 heads (total protein).  There are three breeds of sheep: Sapudi, Dormas (Dormer x Sapudi), and Suffas (Suffolk x Sapudi). Sapudi, Dormas, and Suffas Ewes with only one child each lambing were abbreviated as SAS, DOS, and S...

Nosheen Jehajo* and Naheed Shah

...ng no choice tests, this laboratory study investigated the effect of different temperature (20, 30 and 40°C) and humidity (50, 55 and 60%) regimes on the damage extent and weight loss caused by C. maculatus to some stored pulses i.e. green gram (Vigna radiata (L.) Wilczik), mash gram (Vigna mungo (L.) Hepper), cowpea (Vigna unguiculata (L.) Walp.) and brown colored chickpea (Cicer arietinum (L.). The results revealed that the infestation percentage was sig...

Tasleem Akhtar1*, Muhammad Farooq Nasir2, Imran Bodlah2 and Muhammad Adnan Bodlah3

...eared on A. pisum in the laboratory at 23± 1°C has been studied. The development cycle of A. smithi from larvae to adult was completed in about 11 days. The pre-mating period of males (n=10) varied between 5 and 8 min (mean: 4 min). Copulation time (n = 10 pairs) was between 33 and 55 sec (mean: 45.2 sec). Oviposition time (n = 10 females) was between 1 and 2 sec (mean: 1.5 sec). Female parasitoids lived longer (5.75 days) than male parasitoids (4 d...

Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A

...s at the Avian Influenza laboratory, of the National Veterinary Research Institute, Vom Plateau State. A structured Questionnaire was also administered to 50 poultry farmers. Results revealed an overall sero-prevalence of 5% with H5 and H9 haemagglutinating inhibiting antibody titre ranging from 1 log2-5 log2. Breed specific sero-prevalence were 4% and 16% for local and exotic (layers) chickens respectively, while broilers showed no detectable antibodies. Ques...

Dhurgham K. Seger1, Rahman H. H. Al Qasimi2, Azhar A. Jaffar3, Salah H. Faraj4, Ahmed I. Ateya5* 

... at the biology sciences laboratory of the Marshes Research Center/Thi-Qar University. Sequencing technology was used to detect mutations in SSTR1 gene. The results of the multiple alignment analysis of the sequence of SSTR1 gene indicated the presence of one mutation 264 A>G. The obtained mutation produced three genotypes, namely AA, AG, and GG. The frequency of the genotypes AA, AG and GG were 0.50, 0.17 and 0.33, respectively. While the frequency of the ...

Nazia Haider, Asia Bibi* and Nahid Kausar

...udy was conducted at the laboratories of Zoology department The Women University Multan and the Department of Hematology, Nishter Hospital Multan, from June 2016 to Dec 2019. A total of 500 participants, 300 with spontaneous abortion and 200 healthy pregnant controls in first trimester of pregnancy were included in this study. The data was collected through questionnaire at the time of sampling after obtaining informed consent. ABO and Rh blood group was ident...

Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar

...ree different biomedical laboratories for this study. A total of 4827 suspected individuals were tested, with 2088 (43.2%) of them tested positive for HCV antibodies. Samples were tested against anti-HCV antibodies through PCR and genotype is identified through genotyping method. In the HCV positive sample, genotype 3a was the most common genotype, accounting for 652 (76.3%) of the infected people. Given the enormous morbidity and mortality caused by HCV, havi...

Muhammad Faizan1, Muhammad Nasir1, Muhammad Waqar Hassan1*, Ghulam Sarwar2 and Moazzam Jamil3

...optera: Bruchidae) under laboratory conditions. Biology of beetles was assessed in the form of growth index (GI) which showed maximum GI values in cowpea and mung bean varieties and least in chick pea genotypes and because of this cowpea and mung bean genotypes were classified in susceptible category and chickpea varieties were classified in resistant category. Percent weight loss by C. maculatus was maximum in cow pea and mung bean genotypes and least percent...

Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*

... and humoral response in laboratory animals. Safety test showed no general adverse reactions to the vaccines when injected either into mice or rabbits or cow calves. Challenge protection studies revealed a significantly higher survival rate in vaccinated mice and rabbits compared to placebo groups. None of the vaccines when streaked onto culture media showed any growth indicating sterility of the vaccines. Repeated measures ANOVA was applied to compare mean O....

Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1 and Abdur Rahman Ansari1

...oves through the routine laboratory procedures. The prevalence of Eimeria and Capillaria spp. were recorded as 75% and 25% respectively in district Jhang. Whereas, mixed infection (Eimeria spp. + Capillaria spp.) was not detected in the examined birds. Eggs per gram (EPG) counted through McMaster technique for the Eimeria was 700, while that for Capillaria was 850. Proper control measures, good management and public guidance are needed to control these infecti...

MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR

...sed in quality assurance laboratories of pharmaceutical
industries.
...

Abdiaziz Idiris Mohamud1,2*, Yonis Abukar Mohamed1,3 and Shafii Abdullahi Mohamed1,4

...crucial for effective collaboration and resource optimization. Addressing research gaps, including socio-economic impact assessments, intervention effectiveness evaluations, cultural and contextual considerations, analysis of different production systems, and exploration of the intersection of animal welfare and zoonotic disease prevention, is necessary for evidence-based decision-making. By leveraging research, promoting evidence-based interventions, and stre...

Umar Salisu Ahmad1, Adamu Abdul Abubakar1,2*, Hassan Abubakar Bodinga1, Nura Abubakar1, Ekaete Ime Oviawe1, Zaid Shehu3, Abubakar Musa Mayaki3

...e, foamy salivation, and labored breathing were observed. The prescapular and submandibular lymph nodes were enlarged, and a hard mass was palpable on the distended abdomen. Rectal examination revealed hard rectal feces in rectum were found. The rectal temperature was subnormal (35.4 oC), respiratory rates were 12 cycle/min and the pulse rate was so weak that it was not detectable. Attempts to induce defecation using a KC-bloat failed, and the calf died on exa...

Saman Rizwan and Sohaib Aslam* 

...esearch was conducted in laboratory-controlled conditions using maize residues and a loamy soil from a sugarcane field to determine carbon mineralization patterns. Two different residue management practices were chosen for this study; mulched (soil covered by residues) versus incorporated (residue incorporated in surface soil layer). Carbon mineralization patterns under two treatments were unique from each other. The results showed that all treatments enhance ...

Afsheen Khattak1, Shahida Naveed1*, Naila Khalid1, Inayat Ullah Khan2 and Tamana Bakht

Mukkram Ali Tahir1, Noor-us-Sabah1*, Muhammad Waryam Warraich1, Ghulam Sarwar1, Muhammad Aftab2, Fakhar Mujeeb3, Muhammad Zeeshan Maznoor1, Aneela Riaz4 and Sarfraz Hussain2

...lowed by analysis in the laboratory. All collected data was statistically analyzed for interpretations. It was depicted from results that use of carbon sequestering fertilizer (press mud @ 1 %) along with commercial K fertilizer (SOP) performed superior by resulting in maximum K content in shoot (2.13 %), K content in root (1.12 %) and K content in soil (330 ppm). Similarly, highest fresh weight (42.43 g) and dry biomass (14.37 g) of wheat were also observed w...

Muhammad Luqman1*, Muhammad Talha Shoaib1, Muhammad Yaseen1, Umair Safdar2 and Hassan Raza2

...population as agro-based labor force. Farming community in developing countries is striving to improve agriculture productivity for food security. To gain higher farm yields, farmers use different fertilizers and pesticides to protect their crops from diseases and insect pest attacks. In this perspective, current study was designed to explore negative consequences of pesticides applications on human health and environment in district Sargodha. A cross sectiona...

Muhammad Farooq Bhatti1, Hafiz Muhammad Tahir1*, Aamir Ali1, Hooria Ashraf Khan1, Rabia Fajar Ali2, Ayesha Muzamil1, Fariha Munir3, Fatima Ijaz1, Rizwan Khurshid3

...stify;">Sericulture is a labor intensive cottage industry that has great potential for poverty alleviation. In Pakistan, the farming community so far has not identified the specific race suitable for the specific climatic zones of Pakistan in terms of yield, performance and adaptability. The current study was conducted to find out the optimum hatching and rearing conditions of three silkworm races (Chinese, Bulgarian and Pakistani) and to compare their biologi...

Zakia Panhwar1, Arfan Ahmed Gilal1*, Lubna Bashir Rajput1, Jamal-U-Ddin Hajano2, Shafique Ahmed Memon3, Naimatullah Koondhar4, Muhammad Ibrahim Kubar1

Hina Mumtaz, Muhammad Zeeshan Majeed*, Muhammad Afzal, Muhammad Arshad, Arif Mehmood and Muhammad Qasim
...frugiperda larvae in the laboratory as well as under semi-field and field conditions. All insecticides performed well in controlling S. frugiperda larvae under laboratory conditions. Though, chlorantraniliprole @ 50 mL/100L (58.0–100%), abamectin @ 400 mL/100L (56.0–100%), lambda-cyhalothrin @ 250 mL/100L (52.0–100%) and chlorpyrifos @ 1000 mL/100L (52.0–98.0%) showed significantly (P < 0.05) highe...

Nazia Suleman* and Asia Riaz 

...ey can be mass reared in laboratories for applied biological control against sucking insect pests. Laboratory rearing of ladybirds often need live host, preferably aphids. Artificial diets prepared from different sources do not prove very successful due to complexity of preparation for coccinellid beetles. Short seasonal availability of aphids hinders the rearing procedures. Therefore, some alternate natural dietary sources ...

Shamsher Ali* and Naheed Baloch

...ent was conducted in the laboratory to check the efficacy of essential oils of two indigenous plant species i.e., colocynth, Citrulus colocynthis, and Akk, Calotropis gigantea by using different 03 and 06ml/L concentrations of each plant seed oil against the lesser grain borer, Rhyzopertha dominica, and Khapra beetle, Trogoderma granarium infesting wheat grains. Observations were made on mortality, weight loss, and insect damaged grain. The results show the ma...

Ashfaq Hussain1, Javed Khan2, Ammara Blouch3, Ahmad-Ur-Rahman Saljoqi1, Ashraf Khan4,5* and Zaheer Sikandar6

...n Lipaphis erysimi under laboratory conditions. The mean developmental time from egg till adult emergence was 41.60±0.70 days under laboratory conditions of 24±1c° with 55±5 percent relative humidity (R.H.). The incubation period was 4.00±0.25 days. The pre-oviposition, oviposition, and post-oviposition periods were observed at 8.6, 24.1 and 6.0 days, respectively. The total laid eggs/female w...
Iko-Ojo Charity Ikwe Agada, James Agbo Ameh, Olatunde H. Olabode and Martha Echioda-Ogbole*
...buja based on records of laboratory confirmed CPE cases from 2011- 2021 presented to Veterinary world clinic, Abuja. A total of 197 cases of gastroenteritis were recorded in the years under review, out of which 132 (67%) cases were confirmed positive for CPE using the SNAP parvovirus antigen detection kit (IDEXX Laboratories, United State). Other disease conditions include helminthiasis 25 (12.7%), canine babesiosis 3 (1.5%)...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
... pigeons through routine laboratory methods in district Jhang. Fresh fecal samples (n = 1800) of pigeons were gathered in plastic bags from district Jhang home fanciers and local retailers. Smearing, sedimentation, and flotation procedures were among the various parasitological methods used to analyze all of the samples that were gathered. Herein, the prevalence of coccidiosis was 70.7% and that of capillariasis was 15.7% whereas, mixed infection was 13...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

...ted in a Plant pathology laboratory at Women University of Swabi in Khyber Pakhtunkhwa, Pakistan, to explore the management of seed-borne fungi in maize using castor oil treatment. The study involved collecting and analyzing seeds of various maize varieties such as yellow, blue, white, waxy, and hybrid, sourced from Buner district in KP. Through blotter test and agar plate methods, a total of eight different species of fungi were isolated from five different m...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M. 
...n national and reference laboratories worldwide. In this study a comparison of the specificity and sensitivity between 2 different formats of conventional RT-PCR (one and two steps) and 3 different formats of RRTPCR (one step using TaqMan probe, two steps using TaqMan probe and two steps using hybridization probe) were performed and compared as a diagnostic tool for H5N1 virus detection. All these formats of PCRs appeared within the same specificity for H5 gen...

Abd El-Razakl, A.G. and AboElkhair2, M.

...iate control groups in a laboratory. The immune response on farm A showing higher serological titer at the age of 35 day via ELISA test. Both two vaccines provide complete protection against mortality but not protection against bursal atrophy or histopathological changes after challenge. As well as RT-PCR-based viral IBD detection in bursa of Fabricius at day post challenge.

...

Bakr3 *, Youssef M.; Nour El-Din3, Hanan A.; Abd EL-Wahed2, Waiel F. and Hemeidal , Aläa A.

...e and match the previous laboratory studies which make it one of the best candidates to be immunogenic and capable of producing antibodies that cross react with PLRV. The peptide was chemically synthesized and injected into animal (mouse). The obtained antibodies were tested by using TAS-ELISA and Immuno dot-blot assay. The obtained antibodies were positively reacted against PLRV infected potato tissues.

...

Muhammad Ramzan1*, Unsar Naeem-Ullah2, Shafia Saba2, Madiha Mobeen Khan3, Naeem Arshad Maan3, Asad-ur-Rehman4, Mahreen Khalid5, Syed Waqar Hussain Shah6 and Wardah Muzaffar7

...ns was carried out under laboratory conditions at MNS-University of Agriculture, Multan on Ficus benjamina. All stages from eggs to adult were recorded and observed. The complete metamorphosis like egg, larva, pupa and adult was found in T. varians. The female laid eggs in 2 to 6 layers. The diameter of egg was 0.60 to 0.70mm. There are five larval instars of T. varians. The head length of 1st, 2nd, 3rd, 4th, and 5th instars was 0.30±0.05, 0.35±0...

Alhudaib, Khalid

... then transferred to the laboratory, for total nucleic acids (TNAs) extraction. One hundred mg of leaf veins and or cortical scrapings were used for extraction. Samples were macerated in 1 ml of grinding buffer. TNAs were recovered with a silica-capture procedure and stored at —200C till used. 8-10gl of TNA extracts were mixed with Igl random hexamer primer, (Boehringer Mannheim, GbmH) (0.5 g/gl). RT-PCR assay of leaves extracts of infected fig accession...

Karl Maramorosch

...benefits derived from collaboration between experts working in diverse fields of science and from participation in symposia and congresses.

...

A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1

... strain versus reference laboratory and vaccinal strains of BVDV. Clustering and phylogenetic analyses suggested that these Egyptian vaccinal and field BVDVs are low-Kinetic variants of reference BVDV-Ia. particularly the NADL, represented as a distinguished branch within the phylogenetic tree. Accordingly, BVDV-la (NADL-like), is the dominant circulating genotype in Belbees, El-Sharquia, to date, and the local vaccine could likely induce an antigenically appr...
Geeta Moolchandani1, Jaiperkash Moolchandani2*, Nasima Iqbal3
Syed Mohsin Turab4, Muhammad Kashif5 and Lubna Farooq6
...l Hospital, Karachi. All laboratory tests were done from the central laboratory of the civil hospital. The presence of hypoxic ischemic encephalopathy was staged using the Sarnat criteria at the time of admission. Data was collected on predesigned proforma consisting of demographic variables and the risk factors leading to HIE. Data was entered and analyzed in Statistical Package for Social Science (SPSS) version 20. The mea...
Muhammad Saleem1, Saifullah Khan2* and Mahmood ul Hasan3
 
...tractor, consultant, and labor-related factors have been found as insignificant. The research recommends that project management should make effective contingency planning, experienced ways of acquiring the materials, acquire human capital from foreign markets, external factors, the release of funds on time and raw materials. The results of the study will be helpful for general practitioners consult the problems and recognize the factors associated with the pr...

Ram Prasad Ghimire

...alysis of fodders in the laboratory for nutrient composition, the feeding experiment determining fodder intake and weight gain monitoring in the goats, and a subsequent in-vivo experiment determining the apparent digestibility of fodder nutrients of those browse species. Both of the experiments were conducted in Randomized Complete Block Design (RCBD) with six treatments and five replications on native hill goats of Nepal. The fodders of popular five browse sp...

Nasir Shah1,4, Muhammad Ibrahim1*, Zarnosh Habib2, Kalsoom3 and Zahir Shah4

... fruit fly population in laboratory condition. Thus, these plant extracts could be considered as potent biological insecticides for peach fruit fly owing to field trails.

...

Muhammad Yasin1, Azhar Abbas Khan2*, Sana Rubab2 and Sumaira Maqsood3

... parts of the world. The laboratory study was carried out to investigate the insecticidal properties of Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) in combination with commercial diatomaceous earth (DE) formulation Inert-PMS alone and in integrate manners against these pests. Beauveria bassiana was applied at 1×106 and 1×107 conidia/ml alone, or in combination with DE at 50 and100 ppm. Adult insects were exposed to the treated ...

Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab

...ty of the hydrolysate. A laboratory experimental method using a completely randomized design (CRD) was used in this research. In this study, chicken head protein was hydrolyzed using different combinations of bromelain (b) and papain (p), namely CHP (without hydrolysis); CHP1 (0.25% b and 0.75% p); CHP2 (0.5% b and 0.5% p); and CHP3 (0.75% b and 0.25% p). The data were analyzed using one-way analysis of variance followed by Duncan’s multiple range test. ...

Hossam F. Abou-Shaara

...easured under apiary and laboratory conditions. All feeding types were attractive to bee colonies but bees consumed significantly more yeast and sugar candy within 72h than corn and turmeric. All feeding types did not impair the ability of young larvae to develop into pupae. Bee colonies had high sealed brood area and number of bees in yeast group followed by sugar candy and corn flour. Each of corn flour and turmeric affected the survival ability of bee worke...

Kalsoom Abdulrazaq1*, Bisma Arif1, Rimsha Mehboob2, Asma Mehboob3 

...s various sectors and collaboration with intersectoral strategies, especially between national and international veterinary and public health services. This collaborative approach is crucial for addressing the complex dynamics of zoonotic diseases and mitigating their impact on both human and animal populations.

...
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...l (1250 μg). Specific laboratory protocols of antibacterial and antifungal assays were adopted. Absorbance of the broth bacterial culture was measured after 72 hours by using photo spectrometer at the wavelength of 600 nm (A600). For antifungal assays, the volume and concentrations of extracts used were 2 μl (250μg), 4μl (500μg), 8 μl (1000 μg) and 10 μl (1250 μg). The methanolic extract of Nigella sativa (1μl, 125μg) and water...

Aziz Ahmed1*, Anila Naz Soomro2, Muhammad Ramzan Ali1, Muhammad Aleem Khan1 and Hasina Basharat1

...luated the effect of two laboratory-prepared media and one commercially available media i.e., ACTi fish while control is freshwater on sperm quality parameters i.e., percent motility, motility interval, sustainability, and fertilization rates in Nile tilapia (Oreochromis niloticus). The experimental design was CRD with four treatments and three replicates. Lab prepared (Activation media A and B), one commercially available (Activation media C) and freshwater s...

Bilal Saeed Khan1*, Muhammad Aneeb Shahzad1, Muhammad Irfan Ashraf2, Zahid Mahmood Sarwar3, Muhammad Farooq4 and Awias Rasool5

Iqtidar Hussain1*, Muhammad Inam Ullah Qaisrani1, Abdul Aziz Khakwani1, Zuhair Hasnian2, Umar Khitab Saddozai1, Muhammad Naeem4, Hadia Gul5 and Moneeza Abbass3

...rted in certain crops. A laboratory experiment was carried out to evaluate the germination and germination indices of maize with estimation of maize growth infested with parthenium allelopathy. This trial was conducted Completely Randomized Design with 3 replications. Parthenium dry powder (5, 10, 15, and 20 %) mixed with soil produced variable responses regarding seed germination and seedling growth. Emergence index, mean germination time and vigour index wer...

Yousef Abdal Jalil Fadladdin1, Ateeq Ullah2*, Raheela Nawaz3, Yagoob Garedaghi4, Abbas M.A. Al-Azab5 and Mashael Abdullah Aldamigh6

Saadatin Nurul Jannah1, Edhy Sudjarwo1, Osfar Sjofjan2*

...dy was conducted using a laboratory experiment using garlic peel powder as a material, an active substance content test using a quantitative flavonoid test, and antioxidant activity. Additionally, onion peels are used as a feed additive for broiler chickens to evaluate production performance and blood profile. The material used was 189 one-day-old commercial broiler chickens (unsexing). This study consisted of 9 treatments and 3 tests. The treatment used was T...

Muhmmad Asif1, Khunsa Khakwani1, Muhammad Hasnain1*, Farrukh Ilahi1, Muhammad Hussnain Babar1, Shahid Munir Chuhan1, Jehanzeb Farooq1, Hafiz Ghazanfar Abbas1, Iqra Parveen1, Ghulam Sarwar2, Saeed Ahmad2 and Hammad Hussnain2

... The demands of farmers, laborers who harvest crops, and other investors including those in the cotton industry may be met by the introduction of this variety. The present study signifies for recommended as the most suitable commercial cotton cultivars for agro-climatic conditions of Faisalabad.

...

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Bilques Bano3, Muhammad Muddassir Ali4, Nazia Rubab5, Syed Tasadak Mehdi6, Muhammad Wasim Iqbal7, Kumay Hassan Akhtar8, Aqeel Raza9, Ujala Fatima Shan10, Muhammad Aftab11

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...cts when administered to laboratory animals. Furthermore, upon comparing the effectiveness of the vaccines, it became evident that the Montanide-adjuvanted vaccine elicited significantly higher antibody titers (P<0.05) in comparison to the plain vaccine or the aluminum hydroxide-adjuvanted vaccine. Challenge protection assay revealed that Montanide and aluminum adjuvanted vaccines had 100% survival rate among rabbits compared to 16.7% of placebo group. It w...

Arif Mehmood1*, Muhammad Naeem2, Abu Bakkar Muhammad Raza1, Muhammad Zeeshan Majeed1, Muhammad Irfan Ullah1, Muhammad Asam Riaz1, Ikram ul Haq1 and Waqas Raza1

Sarhad Journal of Agriculture

March

Sarhad Journal of Agriculture, Vol.40, Iss. 1, Pages 01-262

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