Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1
...ity of the ethyl acetate extracts from fruit bodies, mycelia and fermentation filtrate obtained from Pleurotus ostreatus also known as the oyster mushroom. The percent mortality of Macrosiphum rosae (rose aphids) and the LC50 values were calculated and used as an indicative of insecticidal potential. The maximum mortality of aphids was achieved at 80 µg mL-1 and the LC50 for the fruit body extract after 24 HRS was calcula...
Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah
...50 L ha-1, sorghum water extracts @ 15 L ha-1 (twice) and 25 L ha-1 (once and twice) and sunflower water extracts @ 15 L ha-1 (twice) and 25 L ha-1 (once and twice), sorghum water extract @ 15 L ha-1+1.25 L ha-1 Dual Gold and sunflower water extract @ 15 L ha-1+1.25 L ha-1 Dual Gold. In interculturing the land space in between two rows of crop plants was pulverized with the help of local tool spade. The s...
Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
...lamic countries.The root extracts of S. persica has been tested for its anticoccidial activity against Eimeria paillata induced infection in mice jejunum. Three different doses, 300, 600 and 900 mg/kg were used after infection of mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stag...
Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
...psicum annuum methanolic extracts. The extract of Capsicum annuum was prepared by maceration method and the total polyphenolic and flavonoids contents were determined using a spectrophotometric method. The antiradical scavenging property and the maximum nontoxic concentration of the extract was determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against Vero...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...ous and methanolic plant extracts of Camellia sinensis (Green tea), Syzygium aromaticum (Clove) and Mentha piperita (Peppermint) was evaluated against identified isolates. Agar well diffusion assay was used to monitor the antimicrobial activity of these strains both in mono culture and mixed culture. Streptomycin sulphate (10 μgml-1) and Amphotericin B (5 mgml-1) were used as positive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal...
Sidra-Tul-Muntaha1, Muhammad Sagheer1*, Mansoor-ul-Hasan1 and Shahbaz Talib Sahi2
...efficiency of five plant extracts (Azadirachta indica, Melia azadirach, Pegnum hermala, baryosma and Zingiber officinale) and three synthetic pyrethroids (bifenthrin, cypermethrin and deltamethrin) on three geographical populations of Callosobruchus chinensis collected during 2013 from Faisalabad, Multan and Nankana districts of Punjab, Pakistan. Three concentrations of each plant extract (5, 10, 15 and 20%) and synthetic pyrethroids (0.01, 0.02 and 0.03%) we...
S. Bushra1,*, M. Tariq1, M. Naeem1, M. Ashfaq1, I. Bodlah1 and M. Ali2
...erve the effect of plant extracts and semiochemicals on physiology and performance of endoparasitoid of aphids, Diaeretiella rapae. Seven different treatments of semiochemicals and plant extract were applied on aphid pests, Sitobion avenae and Rhopalosiphum padi and parasitoid was released. The data regarding emergence, parasitism, sex ratio, tibia length, adult weight and adult longevity of parasitoid D. rapae after the application...
Zafar Iqbal1* and Muhammad Khurshid2
...(ToLCNDV) from plant extracts of Nicotiana benthamiana plants inoculated with ToLCNDV. To immunocapture the ToLCNDV, two antisera raised against Tomato yellow leaf curl virus-coat protein (TYLCV-CP) and African cassava mosaic virus-coat protein (ACMV-CP) were used. However, immunocapture of ToLCNDV could only be achieved by using the TYLCV-CP antisera followed by PCR detection by using specific and degenerate primers. ToLCNDV is geographically wide ...
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...culated. Aliquots of the extracts at known concentration of chlorogenic acid were then applied against different bacterial strains for antibacterial activity. The minimum inhibitory concentration of isolated compound was also measured. Two antibiotics were used as references compounds for antibacterial activities. The aqueous ethanolic extract having 968.125 µg/ml of chlorogenic acid inhibited significantly the growth of tested bacterial strains. Among t...
K.N. Ahmed, S.H.A. Pramanik, M. Khatun A. Nargis M. R. Hasan
Efficacy of plant extracts in the suppression of insect pests and their effect on the yield of sunflower crop under different climatic conditions
...y of five kinds of plant extracts or botanicals viz., Neem (Azadirachta indica) seed oil, castor ( Ricinus communis) oil, a mixture of Neem seed oil and sesame oil, leaf extracts of custard apple (Annona squamosa ) and Bara Bishkatali ( Polygonum orienta) were tested against the pest infestation. The treatment of custard apple leaf extract produced the most favourable result in respect of pest control and crop yield. The oth...
P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi
Botanicals for control of anthracnose of bell peppers
...fy;">Botanicals or plant extracts from Cathranthus roseus, Coleus aromaticus , Manilkara zapota and Azadirachta indica was studied by poisoned food technique, seed germination and under green house experiments for control of Colletotrichum capsici, fungal pathogen responsible for anthracnose disease in bell peppers (Capsicum frutescence L.). The results showed that all the selected plants have potential to inhibit the radial mycelial growth of C. capsici in-vi...
Anil Sehajpal, Saroj Arora and Parminder Kaur
Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...ungal effect of 44 plant extracts and 8 plant oils against the pathogen Rhizoctonia solani was evaluated by disc diffusion method. Out of 44 plants tested, 36 plant extracts showed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibisc...
Sujoy Mondal and S. S. Ghatak
Bioefficacy of some indigenous plant extracts against epilachna beetle (Henosepilachna vigintioctopunctata Fabr.) infesting cucumber
... viz; Neem Azal, Rhizome extracts of Acorus calamus with petroleum ether as solvent and seed extracts of Annona squamosa with methanol as solvent in controlling epilachna beetle, Henosepilachna vigintioctopunctata, Fabr; Both Neem Azal and seed extracts of Annona squamosa were used at 4ml, 5ml and 6ml (per Lt. of water) while this was 1ml, 2ml and 3ml (per Lt. of water) in case of petroleu...
Shyam Singh and L.P. Awasthi
Plant products for the management of yellow mosaic disease of mungbean and urdbean
...icacy of a few botanical extracts on the yellow mosaic of mug bean. Maximum reduction in disease incidence recorded were 66.70 and 63.65 percent in mungbean and Urdbean, respectively by eight sprays of Clerodendrum aculeatum. Whereas, eight sprays of Boerhaavia diffusa root extract could reduce the disease incidence by 60.27 and 58.20 per cent followed by Azadirachta indica leaf extract by 42.43 and 42.92 percent in mungbean and urdbean, respectively. Maximum ...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
...wth promoting effects of extracts of Trigonella foenum-graecum leaves. The extracts of powdered leaves were obtained in various solvents (petroleum ether, chloroform, methanol, ethanol and distilled water) using hot and cold extraction methods. Phytochemical analysis of leaves was performed on powder and extracts of leaves using already reported methods. Mineral analysis was done us...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
...nt effects of crude leaf extracts of six indigenous plants viz., amaltas (Cassia fistula), datura (Datura alba), neem (Azadirachta indica), niazboo (Ocimum basilicum), yellow kaner (Thevetia peruviana) and safeda (Eucalyptus camaldulensis) against the peach fruit fly, Bactrocera zonata (Saunders), at 2% concentration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used fo...
A. Hamid1,*, M. Ilyas2 and S. Kalsoom3
Saba Irshad1,*, Ayesha Ashfaq1, Ammara Muazzam1 and Abida Yasmeen2
...t-align: justify;">Plant extracts have been used as an active antimicrobial compound since long. The present study was intended to validate the antimicrobial, antidiarrheal and anticancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.), used in Pakistani cuisine to enrich flavour and to treat infectious wounds. Their crude ethanol and methanol extracts were tested against five importa...
Jawad Anwar* and Zafar Iqbal
...ent conditions and their extracts were evaluated, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Eschericia coli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...biocontrol agents, plant extracts etc. In the present study the extract of Citrullus colocynthis in different solvents were evaluated on second instar larvae of H. armigera under laboratory studies for toxicity, sublethal and antifeedant effect. The result showed that ethanol based extract was the most effective to control the H. armigera followed by the ethyl acetate. The sublethal concentration of ethanol based extract of C.
Ayesha Aihetasham1,*, Muhammad Saeed Akhtar1, Maryam Umer1, Khalid Zamir Rasib2 and Muhammad Imran Din3
...n buildings. Efficacy of extracts of Foeniculum vulgare and Ocimum basilicum was studied against this termite. Chemical composition of plant extracts by chromatography-mass spectrometry (GC-MS) revealed five different compounds in F. vulgare: Piperidine, 3-isopropyl, Bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl, Benzaldehyde, 4-methoxy, Estragole, 11-Octadecenoic acid, methyl ester and 9-Octadecenoic acid...
Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2
...the indigenous botanical extracts have been used effectively against various sucking pests of cotton but only few studies have been attempted to observe their action against bollworms. Therefore, the present study has been designed to observe the dual action of transgenic cotton and botanical extracts against pink bollworm during two consecutive growing years (2015 and 2016). The three local ext...
Ayesha Aihetasham1*, Khalid Zamir Rasib2, Syeda Rida Hasan1, Imran Bodlah3
...trations of aqueous leaf extracts of three medicinal plants viz., Carica papaya (paw paw), Helianthus annus (Sunflower) and Bougainvillea glabra (Paper flower) against Heterotermes indicola. The leaf extract of C. papaya caused highest mortality i.e. 100% of 10%, 5% and 3% concentration. Bougainvillea glabra and H. annus caused 100% mortality at 10% and 5% concentration while 96.4% mortality on 3% concentration after exposure period of 10 hours. B. glabra
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...nd petroleum ether (EGE) extracts compared to black cumin (Nigella sativa oil; NSO) and insulin. Alloxan induced diabetic rats were post treated with plant extracts (150 mg/kg) and serum samples were collected from overnight fasted diabetic rats on day 15 to observe thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) ...
S. Shahid Shaukat*, Mian Sayed**, Aly Khan, M. Azhar Khan* and Babar Qazi***
...al nematicides and plant extracts significantly reduced the population densities of all three nematodes. Carbofuran was most effective against all the nematodes. Apart from other three treatments, Tridax procumbens emerged as an effective phytonematicide. Helicotylenchus indicus, Rotylenchulus reniformis and Meloidogyne incognita had similar population density levels and were more or less equally suppressed by all the treatments in the order: Carbofuran > T...
Tariq Mahmood, M. Sudheer Tariq, Khalid Mahmood Khokar, Hidayatullah and Syed Ijaz Hussain*
...ffect of different plant extracts (neem seed, neem leaves and tobacco leaves) and insecticide permethrin dust alone or mixed with dung, were conducted against red pumpkin beetle in the field at National Agricultural Research Centre, Islamabad during kharif 2008. Permethrin (0.5%) alone or mixed (0.05%) with dung ash as dust controlled the attack of red pumpkin beetle on the crop with no mortality of plants. Highest mortality of plants due to foliage eating by ...
Abdul Rehman Roonjho*, Waseem A. Gillani**, Awais Rasool**, Naheed Akhtar**, Tariq Mahmood**, Arsalan A.***, M. Afzal*, Iqbal Khan* , M. Asghar Ranjha**** , M. Irfan**** and Javed Khan**
REPELLENCY EFFECTS OF DIFFERENT PLANT EXTRACTS TO COTTON MEALY BUG, TINSLEY (HEMIPTERA: PSEUDOCOCCIDAE)
... (Asterales: Asteraceae) extracts each in petroleum ether, acetone and ethanol were evaluated at the concentration of 1000, 500 and 250 ppm against cotton mealy bug ( ) in a free choice bioassay for two weeks. The ethanol extract of was the most effective against cotton mealy bug having highest repellency (72.5%) at 500 ppm dose. The lowest average repellency (26.3 %) was observed in acetone extract of at 500 ppm dose....
Aniqa Iram*, Javed Khan**, Nadeem Aslam**, Ehsan-ul-Haq**, Habib Iqbal Javed**, Muhammad Irfan*, Awais Rasool*, Muhammad Ishaque Mastoi** and Sumera Aslam*
EFFICACY OF PLANT DERIVED OILS AND EXTRACTS AGAINST WHITEFLY, BEMISIA TABACI (GENNADIUS) ON SESAME CROP
...the effect of some plant extracts of mint (Mentha spp.) and geranium (Pelargonium graveolens) and soybean oil (Glycine max), mustard oil (Brassica spp.) and taramera oil (Eruca sativa) against whitefly, Bemisia tabaci on sesame crop. The data were recorded 24h before and 24h, 48h, 72h and 168h after application of each spray material. The results showed that whitefly population was significantly suppressed by both the botanical oils and <...
Muhammad Mudasar Aslam ⃰, Muhammad Jamil**, Ijaz Malook**, Amana Khatoon*, Ali Rehman*, Abdur Rahim***, Pirzada Khan*, Shakir Ullah Khan Shakir*, Shahid Irfan*, Faizan Ullah****, Khair Ul Bashar**, Mahideen Afridi** and Shafiq Ur Rehman*
PHYTOTOXIC EFFECTS OF CALOTROPIS PROCERA, TAMARIX APHYLLA AND PEGANUM HARMALA ON PLANT GROWTH OF WHEAT AND MUSTARD
...0% and 25%) of all plant extracts. Roots of both wheat and mustard were highly affected by plant aqueous extracts at all concentrations (5%, 10%, 15%, 20% and 25%) but shoots were inhibited by higher concentrations (20% and 25%). This study revealed that wheat is more sensitive to different plant extracts as compared to mustard. It is thus concluded that inhibitory effect increases with th...
Asif ullah Khan*, Faizan Ullah*, Sultan Mehmood*, Muhammad Irshad* and Farhat Ullah Khan**
ALLELOPATHIC EFFECTS OF JATROPHA CURCAS L. LEAF AQUEOUS EXTRACT ON EARLY SEEDLING GROWTH OF L. PARTHENIUM HYSTEROPHORUS
...ds were found in aqueous extracts and were higher in 25% extract (52.72 mg gallic acid eq. / gm of extract). Higher concentration of the extract (25%) significantly decreased seed germination (%), seed germination index, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, seedling relative water content and seed vigor index. It was inferred from findings of the present investigation that aqueous extract of exhibits inhibitory...
Felicia NkechiEkeh*, Ifeanyi Oscar Ndimkaoha Aguzie, Joy Ihuoma Nzei, Chinenye Maria-Goretti Ohanu, Godwin Ikechukwu Ngwu and ChukwudiebubeUgolo
...> was studied. Ethanolic extracts of P. guineense and C. annum fruits were prepared using Soxhlet extractor. In a split-plot design comprising the two extracts in different concentrations, a total of 63 sundried adult C. gariepinus of mean weight 111.47 ± 2.34g were randomized into three treatment groups A – C for each extract and a control D. The treatment groups were in triplicates with ea...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
... mutans. The natural extracts of Streptomyces strains significantly inhibited and delayed the biofilm formation by S. mutans and a profound effect was observed in case of the extracts of Streptomyces strains HU-09 and HU-10, which delayed biofilm maturation and even decreased biofilm formation with the passage of time. The micro well cytotoxicity assay against Artemia salina showed the cytotox...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1, Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
...e effectiveness of plant extracts to improve the persistence of Spodoptera littoralis multiple nucleopolyhedrovirus (SpliMNPV) against beet army worm, Spodoptera exigua(Hübner) under harsh sunny field conditions in Saudi Arabia. A preliminary test of SpliMNPV was performed to determine the lethal concentrations of this virus against the first and second larval instar of S. exigua. The potency of ten plant
Mudassar Javed1,Muhammad Zeeshan Majeed1,*, Muhammad Sufyan1, Sajjad Ali2 and Muhammad Afzal1
... and different botanical extracts were screened against these pests on okra variety ‘Pusa Sawani’ under field conditions during 2015 and 2016 in a randomized complete block design. The average number of lepidopterous larvae in plots treated with the synthetic insecticides was 1.87 and 2.12 larvae/plant in 2015 and 2016, respectively, compared with 6.5 larvae/plant in untreated control plots. For the botanicals, an average of 2.57 and 2.92 la...
Habib-ur-Rehman1*, Saima Mirza1*, Mansoor-ul-Hasan2, Qurban Ali3, Hafiz Abdullah Shakir4, Muhammad Yasir5
... concentrations of plant extracts and the exposure time. Overall results showed that methanolic extracts were more effective than other three solvents and extract of C. paradise was found comparatively more effective and R. communis was found effective than C. paradise. Repellency varied inversely with increase of exposure time. Hence, these findings underlined the potential repellent effects of both pla...
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
...>N. sativa)alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 µg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) assay. Plaque reduction assay was used to test the mode of action. Out of six...
Xiaqing Chen, Yü Huang and Zhen Huang*
...th and its ethyl acetate extracts caused high mycelial growth inhibition and germination inhibition of S. shiraiana, with the inhibition value up to 80% and 90%, respectively. An apparent increase in mycelial growth inhibition in a dose-dependent manner was observed when ethyl acetate extract and carbendazim were applied individually. The EC50 of T. hamatum ethylacetate extract or carbendazim in mycelial growth inhibition of S. ...
Dan Yü, Chuchu Li, Yü Huang and Zhen Huang*
...rnatant and ethylacetate extracts from T. hamatum inhibited mycelial growth of S. sclerotiorum, with EC50 value of 0.54 µl/cm2 and 2.99 µg/ml, respectively. The sclerotia production of S. sclerotiorum decreased when treated with ethylacetate extracts. The level of additive inhibition on S. sclerotiorum using both fungal ethylacetate extrac...
Bushra Qadir1, Muhammad Asim2 and Mohammad Akmal1*
...ed with overnight in bio-extracts solution made of perennial tree leaves. The study compares early growth performance of alfalfa varieties for forage biomass production. The seed was soaked overnight (14 h) in extracts made from dry powdered leaves of Bakayn (Azadir achtaindica), Ber (Ziziphus mauritiana) and water (H2O) and planted in pots on the next morning. The experiment was conducted in a completely randomized design a...
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
...>T. granarium. Plant extracts were obtained by Rotary Shaker apparatus by using acetone as solvent. Four different concentrations 5%, 10%, 15% and 20% were prepared by diluting in acetone. The data regarding mortality, growth regulation and repellency of T. granarium was observed. Repellency of plant extracts was tested using area preference method while for the growth regulatory potential of plant
Syed Atif Hasan Naqvi1*, Ummad-ud-Din Umar1, Ammarah Hasnain2, Ateeq ur Rehman1 and Rashida Perveen1
... of BLB of rice. Aqueous extracts of fifteen different plant parts either individually or in combination were tested at various concentrations under in vitro conditions by poison food and disk diffusion techniques while best effective seven were trialed in glass house and field experiments to determine their efficacy against Xanthomonas oryzae pv. oryzae. In vitro and in vivo studies under different conditions showed significant response of Mentha piperita, Az...
Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1
Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
... was obtained in aqueous extracts of 2% WM peels and the yeast cells attained up to 33% protein content on dry weight basis. In 2% aqueous extract of WM, the yeast strain yielded maximum biomass up to 6g/L with corresponding absorbance(600 nm) of 3.49±0.05 after 48 h of incubation at 37 ºC, pH 7.0 and under non aerating conditions. These results indicate important biochemical attributes of the abundantly produced fruit wastes and their efficiency f...
Muhammad Muddassir Ali1,2 and İbrahim Hakkı Ciğerci1,*
...the alcoholic and hexane extracts of T. turcica. Methanol extracts showed the highest DNA damage (20±1) at 200 µg/ml concentration and 11.67±2.52 at 50 µg/ml. Ethanol extracts showed the 2nd highest DNA damage (19±2)at 200 µg/ml concentration and 11 ±1at 50 µg/ml. While least was observed in the hexane extract (10.33&plu...
Mirza Imran Shahzad1, Hina Ashraf2,*, Muhammad Arshad3, Sabeeha Parveen4, Amna Aslam4, Nargis Naz4, Zahid Kamran1, Sumbul Gohar Khalid5, Sajid Hameed1, Muhammad Ashfaq6 and Muhammad Mukhtar7
... potential of methanolic extracts of eleven selected Cholistani plants against New Castle Disease Virus (NDV)-LaSota strain. All of these plants were reported for their different pharmacological activities but their antiviral potentials were not known before. The methanolic extracts were made and concentrated by rotary evaporator and finally dissolved in distilled water before taking their antiviral trials in 7-11 days old c...
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
...Piper nigrum leaf extracts were evaluated against S. litura by choice and no choice method under laboratory controlled conditions. In M. oleifera extract, the maximum antifeedant index was recorded in 125.00 mg/ml (64.93±2.61), while minimum antifeedant index was observed in 7.81 mg/ml i.e. 8.39 ± 1.85 by no choice method. In case of M. paniculata extract, the maximum antifeedant index was 98.90±0.67 in 1...
Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2
...onclusion, the ethanolic extracts of A. lebbeck L. and C. sinensis L. showed in vitro anthelmintic activity against H. contortus at concentrations tested.
... 
Hafiz Abdullah Akbar, Zafar Iqbal, Waqas Raza*, MuhammadUsman Ghazanfar and Salman Ahmad
...ffect of different plant extracts /allelochemicals and salicylic acid on the growth of Penicillium digitatum causes citrus green mould by using 7 plant extracts alone and in combination with wax and 3 concentrations of salicylic acid alone and in combination with wax. The study revealed that in case of plant extracts maximum growth inhibition (35.90 %) was shown by Anar followed by ...
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
...ng of aqueous methanolic extracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A. niger res...
Tahira Moeen Khan* and Amat ul Mateen
...gents. In this work leaf extracts of Melia azedarach (commonly known as dharaik) a species of family Meliaceae and Morus nigra (commonly known as Shahtoot) family Moraceae were used for bio reduction of copper ions to CuO nanoparticles in place of harmful and expensive chemical/physical methods. A cheaper, simple to perform method giving high yield without by products is employed here. The leaf extract act both as reducing and capping agent....
Bushra Siddique1,*, Muhammad Tariq1, Muhammad Naeem1 and Muhammad Ali2
...nt combinations of plant extracts and semiochemicals were used for natural enemypreference experiment. The experiment was conducted with seven treatments and five replications at Glass house situated in Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi field area during Feb-April, 2015. The Coccinella septempunctata were collected from wheat crop plants. They remained starved for two days before Olfactometer bioassays. For D. rapae, mummi...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...evaluated the methanolic extracts and essential oils of eight indigenous plant species for their insecticidal potential against 2nd instar D. mangiferae individuals. Standard twig-dip method was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individuals varied with plant materials and increased along with the extract concentration and exposure time. Botanical extracts
Sadaf Rahim and Mudassar Iqbal*
...re. Increased potency of extracts was observed with the increased exposure time. The LC50 value calculated for organic extract obtained from T. harzianum grown on PDB against Diuraphis noxia was 398.69 μg mL-1 after 3 hours with increased potency (237.46 μg mL-1) after 24 hours, whereas against Tribolium castaneum it was 213.85 μgmL-1 after 24 hours while its decreased potential was recorded (1153.5 μg mL-1) after 3 hours. Similar pattern of toxici...
Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed
...e toxicity of methanolic extracts of eight flowers i.e. Mexican marigold (Tagetes lucida), African marigold (Tegates erecta), tecoma (Tecoma stans), calendula (Calendula officinalis), basil (Ocimum basilicum), oxeye daisy (Leucanthemum vulgare), lily (Lilium longiflorum) and chrysanthemum (Glebionis segetum) against the worker individuals of subterranean termite Odontotermes obesus (Isoptera: Termitidae). Bioassays were conducted using filter paper disc method...
Syeda Naila, Muhammad Ibrar, Fazal Hadi* and Muhammad Nauman Khan
... screenings of different extracts of Oxalis pes-caprae L. showed the presence of carbohydrates, phenols, flavonoids, tannins, saponin, fats and oils ...
Ghulam Hussain1, Muhammad Asrar1, Dilbar Hussain2, Khurum Zia3, Abdul Rashid4*, Hina Anwar5, Muhammad Azeem1, Saddam Hussain1 and Sabeen Asghar1
...genously available plant extracts. The efficacy of following insecticides and plant extracts viz. Cascabela thevetia leaf extract @ 300ml/acre, Azadirachta indica @ 300ml/acre, Profenofos @600ml/acre., Crown 70WS @125gm/acre, Match 50EC@250ml/acre, Helmat 40EC@600ml/acre and Confidor 20SL@ 250ml/ acre were evaluated on cotton against 3rd instar cotton mealy bug. Data was collected after 3,6,12,24 and 48 hours. Maximum mortal...
Waseem Abbas1, Shakeel-ur-Rehman2, Abdul Rashid2, Muhammad Kamran3, Muhammad Atiq2 and Muhammad Ehetisham ul Haq3*
...isease incidence. Leaves extracts of four plant extracts i.e. Calotropis gigantea, Zingiber officinale, Allium cepa and Azadirachta indica were evaluated at 3 % concentration against eggs hatchability and adult emergence of the whitefly in lab condition. Two consecutive sprays were applied to assess the relative impact of different plant extracts against adult whitefly population and the d...
Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*
... potential of H. gilesii extracts in different solvents was assessed using agar well diffusion method against bacterial and fungal strains, while cytotoxic activity was studied in the methanolic extract using brine shrimp’s lethality assay. All the extracts showed significant biological activity against Gram positive, Gram negative bacteria and selected fungal strains. Acetone, chloroform and methanolic
Sumaira Akram1, Sajid Aleem Khan1*, Nazir Javed1 and Saeed Ahmad2
...control agents and plant extracts. Among chemicals, cartap was most effective with lowest egg hatching and among biocontrol agents Paecilomyces lilacinus (Thom) Samson (PL) produced highest egg hatching while, Trichoderma harzianum Rifai (TH) caused lowest egg hatching. In plant extracts the highest egg hatching was recorded in clove at S concentration and lowest by neem extracts...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...ects of methanolic crude extracts of Ipomoea stolonifera on 4th instars larvae of both laboratory-reared and field-collected Aedes aegypti were carried out. The 4th instars larvae of laboratory-reared and the field-collected A. aegypti mosquitoes were exposed to 5000mg/L, 2000 mg/L, 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L of the extract. The solvent for the extract, dimethyl sulphoxide (DMSO) was used as control. The r...
Ahmad-Ur-Rahman Saljoqi1, Muhammad Zubair Khan1, Ayesha Bibi2, Muhammad Shehzad Khan1*, Bashir Ahmad1
...nt doses of boron, plant extracts and a bactericide in different combinations on tomato plants. This research study was performed at Agricultural Research Institute, Tarnab, Peshawar-Pakistan. Different biochemical tests were conducted to confirm stem rot pathogen. Boron was applied at the rates of 1.97, 2.96 and 3.95 g seedbed-1. Plant extracts were neem (Azadirachta indica) and ghwaraskay (Dodonaea viscosa). Morphological ...
Bilal Atta1*, Muhammad Rizwan1, Arshed Makhdoom Sabir1, Muhammad Dildar Gogi2, Muhammad Sabar1, Bakhtawar3, Faizan Ali3 and Mehran Sarwar3
...icacy of different plant extracts viz. Ginger (Zingiber officinale), Neem (Azadirachta indica), Clove (Syzygium aromaticum) and Tobacco (Nicotiana tabacum) was compared against T. castaneum infesting stored wheat. The results have suggested that mortality and repellency in T. castaneum increased as the dose rate of crude plant extracts and exposure interval increased. The maximum mortality (86.67%) was achieved with the high...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
...yl acetate and ethanolic extracts of Z. mauritiana var. spontanea measured the highest zone of inhibition against Staphylococcus epidermidis, Escherichia coli and Salmonella typhi. The MIC values of ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea were the lowest for Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, Shigella flexneri ...
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
Muhammad Salim1*, Ayhan Gökçe1, Muhammad Nadir Naqqash1 and Orkun Ersoy2
...t is combined with plant extracts. ...
A. S. M. El-Nuby1† , S. A. Montasser2 and I. A. El-Khadrawy
Control of root-knot nematodes using wild plants colonized Sinai, Egypt
...ivity of some wild plant extracts located in North and South Sinai, Egypt was examined against root-knot nematode (RKN). The selected seven plants viz., Artemisia judaica, A. monosperma, Bassia muricata, Cornulaca monacantha, Salsola kali and Zygophyllum album with different dilutions (25%, 50%, 75% &100%). The potent nematicidal efficacy observed in the extract of A. judaica followed by A. monosperma. In vivo trial post inoculation treatments were effecti...
Syeda Farzana Bibi* and Siraj-ud-Din
Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
... present study ethanolic extracts of leaf and bark parts of C. adansonii were evaluated through cytotoxic activity. Brine shrimp larvae assay was conducted for this purpose. Extractive values of leaf and bark parts were estimated using n-hexane, chloroform, ethanol, methanol, water, and ether as a solvent. 10g of plant powder was dissolved in 200 ml solvent during various extract preparations Fluorescence study was conducted after treating ...
Gregory Ejikeme Odo1,*, Juliana Ekenma Agwu1, Nkechi Nweze2, Clara Ikegbunam2,Felicia Ekeh N1, Vincent Ejere1, Ngozi Ezenwaji1, Reginald Njokuocha2, Godwin Ngwu1 and Fabian Okafor1
...ffects of ethanolic leaf extracts of Duranta erecta (L) in 48 albino wistar rats, Rattus norvegicususingbiochemical and haematological indicesof toxici
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2, Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
Iram Bilqees1, Yasir Iftikhar1, Mustansar Mubeen1,2*, Qaiser Shakeel3, Ashara Sajid1, Zahoor Hussain4, Aqleem Abbas2, Muhammad Aamir Sohail2, Judith Jeruto Kiptoo5 and Shehzad Iqbal2
Use of Nutrients and Plant Extract to Manage Okra Yellow Vein Mosaic Disease (OYVMD) in Sargodha, Punjab, Pakistan
...nts along with the plant extracts to control these diseases to enhance the okra production....
Rabab Rafaqat1, Habib Ahmed Rathore1, Tariq Masud2, Imran Hayat1* and Imtiaz Hussain1
Determination of Different Chemical Constituents of Fruit, Leaves and Oil of Olea cuspidata (Wild Olive) Grown at Rawalakot, Azad Jammu and Kashmir
...TFC) of fruit and leaves extracts in these solvents were determined. Oil was extracted from wild olive fruit by the process of solvent extraction and was studied for physicochemical properties like refractive index, specific gravity, pH, free fatty acid, peroxide value, iodine value, saponification number and unsaponifiable matter. Results indicated that fruit and leaves of wild olive grown at Rawalakot Azad Jammu and Kashmir had moisture content (48.33±...
Tahseen Ullah* and Noor ul Amin
In Vitro Antibacterial Activity of Medicinal Plant Extracts
...s (low, 5 mg/ml and high extracts, 300 mg/ml) of these plants were evaluate against two different bacterial strains i.e. Bacillus subtilis and E. coli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for i...
Malik Abdul Rehman1, Shafqat Ali1, Muhammad Babar Shahzad Afzal1, Muhammad Nawaz Khan1 and Mujahid Ali2*
Efficacy of Chemicals and Botanical Extracts to Control Citrus Canker on Kinnow in Sargodha Region
...hemicals and plant-based extracts to find suitable chemicals against the canker in Kinnow. The study was carried out at the citrus farm of the Citrus Research Institute, Sargodha, Punjab-Pakistan during 2011-13 on ten-year-old bearing trees of Kinnow plants. There were nine treatments, control (no chemical was applied), Neemsol @ 5 ml, copper oxychloride @ 3 g, streptomycin sulphate @ 1 g, potassium acetyl benzoic acid @ 10 ml, difenoconazole @ 0.5 ml,
Muhammad Imran1,2*, Shahbaz Talib Sahi1, Muhammad Atiq1 and Amer Rasul3
Brown Leaf Spot: An Exacerbated Embryonic Disease of Rice: A Review
.... While, the use of phytoextracts and antagonist are considered to be safe, eco-friendly, cost-effective economically and biodegradable. The use of plant activators as another new strategy that activates the defense system of plants and reduces the disease. The plants which are scarce nutrients are more prone to disease as compared to nutrient deficient. The pathogen damage is compensating by a specific nutrient that reduces the disease through tolerance. Good...
Abida Parveen1, Sajid Aleem Khan1, Nazir Javed1, Anam Moosa1*, Khushboo Javaid1, Hina Safdar1 and Asma Safdar2
...nga oleifera L. (MO) extracts and Steinernema kraussei Steiner (SK) as stand-alone treatments and in combination was assessed to suppress the reproduction and development of MI. Highly susceptible variety of tomato cv. ‘Money Maker’ was tested for the invasion and development of MI. Aqueous and ethanolic MO extracts were tested in combination with SK at three different concentrations and time intervals...
Syeda Farzana Bibi* and Siraj ud Din
Unraveling the Bioherbicidal Potential, Elemental Analysis and Nutritional Evaluation of Crataeva adansonii Dc Leaf and Bark
...ts of the proposed plant extracts were determined, including percentages of carbohydrates, proteins, fats, fiber, ash, and moisture. The bioherbicidal potential of the proposed plant was evaluated through the Lemna minor model of phytotoxicity. C. adansonii is a source of several essential macro and micronutrients. A nutritional value analysis of this plant offers its use as a food source. Bioherbicidal/phytotoxic activity revealed significant results in the f...
Ayesha Talib1,2, Khanzadi Nazneen Manzoor1, Wajahat Ali1, Maria Saeed1, Muhammad Asif Gondal1, Malik Badshah3 and Abid Ali Khan1*
...d from the aqueous fruit extracts of Ficus sycomorus and their physico-chemical properties as well as antimicrobial activity against Multidrug Resistant (MDR) bacteria is evaluated. The total formation of the Cu nanoparticles was observed visually with a color change and confirmed by the appearance of peak through UV-Vis spectroscopy analysis. The electron microscopy imaging revealed that the biogenic Cu nanoparticles were spherical in shape and had a d...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...size: small;">Methanolic extracts and essential oils of ten indigenous plant species were evaluated for their insecticidal and repellency potential against the subterranean termite Odontotermes obesus Ramb, a destructive insect pest of wooden infrastructures, agricultural crops, orchards and forest plantations. Standard filter paper disc method was used for both toxicity and repellency bioassays according to completely randomized design. The response of...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...nge of Pakistan. Acetone extracts (10%) of forty plant species were evaluated against Asian citrus psyllid (Diaphorina citri), armyworm (Spodoptera litura), house mosquito (Culex quinquefasciatus) and subterranean termite (Odontotermes obesus) using twig-dip, leaf-dip, aqueous exposure and filter paper-dip bioassay methods, respectively. Results revealed that the extracts of Mentha longifolia
Muhammad Shahzad Akbar, Farrukh Sajjad, Muhammad Afzal, Muhammad Luqman, Muhammad Asam Riaz and Muhammad Zeeshan Majeed*
Field Evaluation of Promising Botanical Extracts, Plant Essential Oils and Differential Chemistry Insecticides against Subterranean Termites Odontotermes obesus (Isoptera: Termitidae)
...some promising botanical extracts (Dodonaea viscosa, Gardenia jasminoides and Nerium indicum), plant essential oils (Allium sativum, Citrus aurantium and Cymbopogon citratus) and differential-chemistry insecticides (chlorantraniliprole, chlorfenapyr, emamectin, indoxacarb, pyriproxyfen and triflumuron) against subterranean termites (Odontotermes obesus) attacking sugarcane crop. Setts of sugarcane variety HSF555 were sown on the ridges under RCB design after t...
Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3 and Waqas Raza1*
Influence of Resistance Inducers on Nitrogen, Phosphorus and Potassium Contents of Susceptible Chickpea Cultivars after Inoculation with Ascochyta rabiei
...hydroxide (KOH) or plant extracts of neem (A. indica A. Juss.), datura (D. metel L.) and garlic (A. sativum L.) were investigated 7 and 14 days after inoculation with Ascochyta rabiei. Elevated nitrogen (2.95%), potassium (1.05%) and potassium (533.00 ppm) contents in the tissues decreased disease severity (79.3%) in cv. C-44. These increases were significant (P ≤ 0.05) after 14 days of inoculation with plant pathogen in plants pretreated with chemicals. Th...
Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Mushtaq Ahmad1, Muhammad Saleem2, Ali Aziz2, Irfan Rasool2, Zeshan Ali3 and Muhammad Amin2
An Update on Biology, Extent of Damage and Effective Management Strategies of Chickpea Pod Borer (Helicoverpa armigera)
...l measures (use of plant extracts, virus/bacteria based insecticides). In case of pod borer outbreaks, chemical insecticides remain as last option for farmers. However, management of chickpea pod borer through use of resistant cultivars, adopting recommended cultural practices and biological control measures have been found more effective, economical, sustainable and eco-friendly....
Muhammad Shahid1*, Unsar Naeem-Ullah1, Waheed S. Khan2, Shafqat Saeed1 and Kashif Razzaq3
Application of Nanotechnology for Insect Pests Management: A Review
...bacteria, viruses, plant extracts, fungi, and protein. This review article climaxes the latest mileposts accomplished for the production and imminent perspective of nanoparticles for the controlling of insect pests. ...
Javeria Afzal, Muhammad Abid, Faisal Hussain* and Alia Abbas
...reas of Karachi. Aqueous extracts (2, 6 and 10%) of these five plant species were made for the experiment. All selected plant species inhibited egg hatching and caused larval mortality. Maximum reduction (24.3%) in egg hatching was recorded in C. album stem extract at 2% concentration. The minimum reduction (0.33%) was observed in the leaf extract of A. viridis at 10% concentration after 48 hours of exposure time. In larval mortality test, maximu...
Muhammad Ayub1*, Munir Hussain2, Sher Ahmad2, Syed Arif Hussain Rizvi3, Shahid Hussain1, Muhammad Qasim2, Muhammad Din2, Muhammad Ishaque Mastoi4 and Tajudin2
...of white grub. Fermented extracts of four herbs Sophora alopecuroides, Artemisia sieversiana, Peganum harmalla and Tagetes minuta were evaluated against white grub under field conditions for two consecutive growing seasons during 2018 and 2019. The minimum per cent infestation, number of white grubs per plot, yield loss and maximum tuber yield was recorded in plots treated with S. alopecuroides fermented extract and Refree.&n...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...ernatants and the EthOAc extracts derived from the MlUV-40b mutant were 1.2-3 times more potent than that of the wild type. A simple approach coupling mutagenesis and growth & second metabolites were used to isolate strains with increased stress resistance and virulence. Increased virulence in some of the mutants correlated with increased insecticidal activity in cell-free extracts that could potentially be used directly...
Muneer Abbas1*, Dilbar Hussain2, Muhammad Saleem2, Abdul Ghaffar2, Sohail Abbas3, Niaz Hussain1 and Abdul Ghaffar1
...in based baits, D= plant extracts and E= A+B+C+D were used during 2015-16. Data of % infested fallen fruits, total flies/trap, total flies captured/year, % fruit punctures, pupal population, % fruit infestation and market value of fruits was collected at regular intervals. Results indicated that when all the components were applied in a combined way they gave significant reduction of fruit losses. As a result of continuous sanitation practices the fruit fall r...
Ahmad Ur Rahman Saljoqi, Muhammad Salim* and Iftikhar Ahmad
...tin along with botanical extracts are effective in controlling T. tabaci infestation, and hence may be used in garlic crop for managing this serious pest.
...
Hagar E. Mohammed1, Ranwa A. Elrayess2 and Heba N. Gad El-Hak2*
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
Safdar Hussain1, Muhammad Naeem Mushtaq5, Ali Bakhsh3, Muhammad Mudassar Maqbool1, Muhammad Sarwar1, Muhammad Jan4*, Muhammad Abdul Qayyum2 and Arif Husain2
...wth regulators and plant extracts. Wheat genotypes (Triple dwarf-1, Aas-2011; Faisalabad- 2008) were exposed to critical drought stage. The plants were exposed to normal irrigation, application of bio stimulants like 2µM ABA, 10 mM SA, 15% MLE and 10% MBLE were applied at grain filling stage but skipping irrigation. Maximum growth related parameters were observed by applying full irrigation. Comparing the bio stimulants application, the application of AB...
Muhammad Usman Ghazanfar1, Waqas Wakil2, Shahbaz Talib Sahi3, Waqas Raza1* and Mahmood Ahmad Randhawa4
...trend was shown by plant extracts where in neem extract induced higher amino acid contents. The cultivar C-44 responds efficiently as compared with other two cultivars. The results of the current study showed that amino acids play significant role in resistance and susceptibility of chickpea cultivars against A. rabiei....
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
Bushra Baig and Saeeda Yousaf*
...nd aphid).The methanolic extracts of Black pepper and Neem were analyzed for identification of phytochemicals by using Gas Chromatography-Mass Spectrometry. The result showed that Alkaloids, Flavonoids, Tannins, Terpenoids and Phenolic compounds were found both in Black pepper and Neem.Quantitative analysis indicated 21 active componentsin Black pepper which were dominated byCaryophyllene (32.3%), Delta-3-carene (18.7%) and Cinene(15.5%). Neem showed 17 active...
Qaiser Shakeel1*, Rabia Tahir Bajwa1, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4
...tion consisting of crude extracts of different plants and fungicides were performed for determination of antifungal activity against Penicillium digitatum, a plant pathogenic fungi responsible for ginger soft rot. Petri dish were used to conduct bioassay through poisoned food technique with triplicates. The crude extract of all the plants showed a better inhibitory effects on the pathogen’s (P. digitatum) mycelial growth rather than fungicides. Among all...
Khan Tamoor1*, Hafsa1 and Hanif Maryam2
...chistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum mortality of 76% by Z. multiflora and F. oopoda at highest dose of 3 g/ liter which was followed by 72% of N. cateria and 49% by A. santolina at the same dose. However,...
Muhammad Irfan Ullah1*, Sana Majeed1, Muhammad Arshad1, Nimra Altaf1, Muhammad Luqman2, Asad Abdullah1 and Muhammad Afzal3
Samra Aftab1, Saleem Ullah1 and Farida Anjum2*
...ultures and their enzyme extracts to check their effect on cellulose hydrolysis. Microbes were isolated from soil by using serial dilution method and were selected on efficiency basis (screening of microbes on the basis of their activities as cellulose degrading agents). The cellulose and related constituent of saw dust and sugarcane bagasse showed that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7....
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...fects of ethanolic crude extracts of cumin (Cuminum cyminum) and jir (Artemisia scoparia) on the performance of broiler chickens. The study used 120 unsexed Hubbard one day old broiler chicks randomly divided into three groups of 40 chicks that were each further divided into four replicates of ten chicks. Extracts were introduced at 200 mg/Kg as feed supplement to two groups of broilers and the third group was ...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...ize: small;">Since plant extracts have long been known to be chitin inhibitor and agents for developmental arrest, chitinase genes and genes that are vital for starting the differentiation program of cells at discrete steps during development are major targets for biocide plant extracts. The current study evaluates the in vitro toxic effects of golden piller (Cupressus macrocarpa) and galangal (Alpinia officinarum<...
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
...The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2). The results of monitoring TLC suggest that callus obtained from Schenk and Hildebrandt (SH) media can proceed to the suspension culture stage because it showed brighter fluorescence spot than other callus and plant extracts, especially for rosmarinic acid....
Sallam A.A.A.1, Hoda M.A.Waziri2,E. K .F. Elbeshehy1, SamiaI.Massoud,1 and Abeer M. Abo El-Wafa2.
...ion.The effectiveness of extracts from garlic
cloves (GE) and onion (OE) as an antiviral against BBMV infection in vivo has been
evaluated. The percentage of virus inhibition induced by GE and OE varied according
to the time of treatment (1, 2 and 3 days).GE was more effective in reducing the
percentage of infection produced by BBMV on faba bean plantsthan did OE.
...
Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2
...ayed by ELISA on protein extracts isolated from same transfected plant tissue samples as for the RT-PCR assays. Results showed that the HSV-gD2 construct was expressed at both the RNA and protein levels in the transgenic tomato plants. Finally, we report here, the use of stable transformation to develop transgenic tomatoes expressing the biologically active HSV-2 gD antigen (gD2). Thus, our results may have implications for the development of plant based vacci...
Hanaa H. A. Gomaa1; Entsar A. A. Nassar2; K. A. El-Dougdoug3
... milk thistle methanolic extracts in Vero cells did not show any morphological difference compared with control. Antiviral affects of milk the most potent methanolic solutions of milk thistle that have antiviral activity with different degrees, line 32 with (HAV), line 9 with (COXB4) and, line 28 with (HSV1)....
Shahida Sabir1, Anwar Ali Shad1, Tariq Masood1*, Zafar Ali Shah1, Nasiruddin2, Yaseen Ahmad1 and Syed Sartaj Alam3
...ycosides were present in extracts at moderate to high concentrations. Methanolic fraction of mushroom proved to be bactericidal with highest % inhibition (65, 36 and 75 %) recorded at 25 mg/L concentration against all the three bacterial strains i.e., E. coli, Staphylococcus aureus and Xanthomonas sp., respectively. Maximum % inhibition (about 50%) was recorded at 25 mg/L concentration against all the three fungal species (Penicillium claviforme, Aspergillus f...
Hassan Shahzad Khan1, Muhammad Tariq1*, Tariq Mukhtar2 and Asim Gulzar1
... toxicity of eight plant extracts and their green synthesized nanoparticles was evaluated against A. albopictus. The maximum mortality of 88% of 3rd instar larvae of A. albopictus was caused by datura extract followed by neem and clove. On the other hand, neem and clove extracts caused the maximum mortality (80%) of 4th instar larvae followed by datura extract (78%). Similarly, the ...
Haroon Ur Rashid1*, Nazia Tahir2, Muhammad Zamin3, Naveed Shehzad4, Aman Ullah2, Bibi Zainub5 and Farooq Azam6
... mulches and their water extracts integrated @ 15L each +atrazine @ 0.125 kg a.i ha-1(1/4th of the recommended dose) was assigned to sub plots (Factor B). Data were recorded and analyzed for dry biomass (g) of total weeds 60 DAS, leaf area (cm2), leaf area Index (%) and Stover yield (Kg.ha-1). Integration of tillage regimes and various allelopathic treatments had significant effect on tested parameters. However, the efficacy of surface mulches was more pronoun...
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
...ults showed that the two extracts of O. aristatus resulted from in vitro culture containing rosmarinic acid and sinensetin. When compared to the wild type, the chromatogram had a larger area than the wild type. This study provides new information regarding the secondary metabolite content of two O. aristatus from in vitro culture....
Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2
...efficacy of desert plant extracts against basal rot of onion in the laboratory, greenhouse and field. Completely randomized design (CRD) was used for in-vitro study and randomized complete block design (RCBD) for greenhouse and field experiments. Three desert plants i.e. Gossypium thurberi, Calotropis procera and Suaeda fruticose were used at 50 ppm, 100 ppm and 150 ppm concentrations. Maximum basal rot disease reduction (78.6%) was recorded at 150 ppm concent...
Tanmoy Mandal1 and Sunil Kr. Ghosh2*
...tin/polygonam/spilanthes extracts will be environmentally sound and eco-friendly and is recommended for leaf miner control to promote organic farming.
...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...tial toxicity of acetone extracts of 40 indigenous plant species of Soon valley and surrounding salt range (Punjab, Pakistan) against D. citri and A. gossypii using standard twig-dip and leaf-dip bioassay methods, respectively. Results of initial screening bioassay showed the highest mortality of D. citri by 10% extracts of Mentha longifolia (L.) Huds. (93%), Melilotus officinalis (L.) Pall. (91%), Nerium indicum Mill. (89%)...
Dilek Şahin1*, Meryem ÖZ2, Orhan Aral2, Mehmet Bahtiyar2 and Selda Taşçi2
...amount of purslane plant extracts (3%, T3; 6%, T6; 9%, T9) and each group had 3 replicates. The fish with an average live weight of 2.55±0.08 g were selected randomly from a fish stock of 250 fish and were 13 fish were placed in each aquarium (15 L). The growth, survival and coloring parameters were determined at the end of 60th day of the study. The best growth parameters were recorded in the T9 group. The best weight gain, specific growth r...
Salma Javed1* and Daniyal Siddiq2
...us, ethanol and methanol extracts were investigated for managing the population of Meloidogyne javanica, in tomato. In laboratory conditions second-stage juveniles (J2) mortality was recorded after 24, 48 and 72 h of incubation in concentrations of 0.25, 0.5 or 1%. Maximum mortality was achieved with ethanol extracts followed by aqueous and methanol extracts. In the greenhouse, leaf
Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1
... acetonic and methanolic extracts of Solanum nigrum, Atropa belladonna, Hyoscyamus muticus, Capsicum frutescens, Datura innoxia and Withania somnifera were tested against root-knot nematode, Meloidogyne javanica. All the tested materials affected the survival of the nematode juveniles and egg-masses hatched depending on materials property, concentrations and solvents used in extraction. The aqueous extract had the best percentage of mortality and inhibition of...
Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah
...n 50 EC) and the aqueous extracts of three local plant species (i.e. Eucalyptus camaldulensis Dehn., Citrus limon L. and Azadirachta indica A. Juss) against the grubs and adults of E. vigintioctopunctata. Three concentrations of each synthetic insecticide (i.e. 2.5, 2 and 1.5%) and botanical extract (i.e. 10, 7.5 and 5%) were bioassayed using standard leaf-dip method. Results revealed that spinosad and 10% A. indica aqueous extract exhibited maximum mean cumul...
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
...>-1) of red clover extracts and 50 µg L-1 17 β-Estradiol serving as positive control were applied by immersion method on C. gariepinus larvae for 30 days, and the effects of red clover extract on sex reversal and gonadal development were also examined at the end of 120 days.At the end of experiment, highest feminization (89%) was observed at 50 mg L-1 red clover group. Morphological and histological examinations of...
Hailemariam Assefa1*, Tesfahun Dagnaw2
...ected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested fungal species. The oil was obtained by soxhlet extraction method. The maximum oil yield using petroleum ether was found 18.4% at a temperature of 70oC and a minimum yield of 13.5% at 30oC while at the same operating condition ethyl acetate and methanol resulted a maximum yield of 17.0% and 16.5% and a minimum of 12.0% and 13...
Aftab Ahmad Sheikh1, Khalil Ahmed1*, Belqees Akhter1, Ghulam Qadir1, Muhammad Qaisar Nawaz1, Hafeezullah Rafa1, Abdul Wakeel1, Abdul Manan Saeed2
...der yield, nitrogen free extracts (NFE), crude fat, crude protein, crude fiber, phosphorus, and calcium contents were envaulted at the physiological maturity of crop, four months after the sowing of crop. Results revealed that water salinity adversely affected the quantitative and qualitative attributes of sorghum crop and negative effects were more pronounced with higher level of salinity ECiw (10 dS m-1). Irrigation with higher level of salinity ECiw (10 dS ...
Pham Tan Nha*, Le Thu Thuy
...shrimp soluble hydrolyte extracts of Hoa Lan ducks. It was a complete randomized design with 5 treatments corresponding to 5 diets and 3 replications. Ten ducks balancing in sex were used in one experimental unit. The treatment 0 (basic diet, 0%). Treatment 1, 2, 3 and 4 were mixed basic and shrimp soluble hydrolysate with 1%, 2%, 3% and 4%, respectively (DM). The treatments were: SH0, SH1, SH2, SH3 and SH4, respectively. The results shown that daily weight ga...
Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3
...the efficacy of n-hexane extracts of two medicinal plants, Artemisia scoparia and Anisomeles indica against larvae, pupae and adults of Culex quinquefasciatus. The study also evaluated the predatory effects of the diving beetle, Agabus cybister, against various instar larvae of Cx. quinquefasciatus. Bioassay of whole-plant extracts was performed following WHO methods, with slight modifications. LC50 values for A. scoparia an...
Lethal and Sub Lethal Effects of Plant Extracts and Green Silver Nanoparticles against Culex pipiens
Abdul Jabbar1, Muhammad Tariq1*, Asim Gulzar1, Tariq Mukhtar2 and Tayyaba Zainab3
...pesticides such as plant extracts and green synthesized nanoproducts have received much attention as potentially useful bioactive compounds against mosquitoes. In the present study, the effects of extracts of four plants i.e. Azadirachta indica, Zingiber officinale, Syzygium aromaticum and Datura stramonium and their green synthesized silver nanoparticles (AgNPs) were evaluated against 3rd and 4th instar larvae of C. pipiens...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
... cultured with scaffolds extracts. Also, the antitumor activity of the scaffolds was studied against cancer osteosarcoma cell lines using Sulforhodamine B (SRB) assay. The results showed that addition of cerium-doped nanobioactive glass to the composite scaffolds was triggered an increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO2 innanobioacti...
Afsheen Noman Saddar1, Anila Naz Soomro2*, Sadaf Tabasum Qureshi1, Syeda Saleha Hassaney1 and Mukhtiar Ahmed Mahar2
... µg/ml) of aqueous extracts of five fertility enhancing plants were tested for 24 h incubation to check mortality of brine shrimp nauplii. Mean comparison of brine shrimp cytotoxicity assay revealed noticeable deviations from the both positive and negative controls. Alive percentage of nauplii observed in the aqueous extract of sweet flag root and radish seed ranged from 0 to 10%, land-calotrops root extract from 0 to 22.5%, peppermint leaves extract fro...
Hafiza Mehwish Iqbal1, Qurrat Ul Ain Akbar1, Saqib Arif1, Shahid Yousaf2*, Salman Khurshid1, Saqib Jabbar2, Neelofar Hamid3 and Uzma Sitara1
... in methanol and acetone extracts. It was concluded from the results that ripening stage has effects on the nutritional values which point out that the ideal maturity stage to maintain optimal postharvest storage ability and nutritional value is breaking stage of fresh tomato which is the most suitable for storage....
Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2
...tial of four local plant extracts and two promising microbial formulations against 5th instar larvae of T. granarium. Toxicity bioassays revealed that the extracts of Citrus reticulata L. and Solanum nigrum L. were most effective against T. granarium causing significantly higher larval mortality (35 – 40%) than other botanical treatments. Similarly, the highest concentration (15%) of C. reticulata extract exhibited max...
Doaa Khairy1, Mohamed Ali Osman2 and Fatma Abdel Mohsen Mostafa1*
...otential of aqueous leaf extracts of moringa (Moringa oleifera Lam), or neem (Azadirachta indica A. Juss) singly or in integration with different parts of canola (Brassica napus L.) extracts to alleviate the deleterious effect of Meloidogyne incognita as well as ameliorate tomato growth in vivo. A mixture of moringa or neem aqueous leaf extracts with different parts of canola viz. leaf, st...
A. Samy1*, H.M.A. Hassan1, Fatma T.F. Abd-El Ghany2, Shama H. Morsy2
...ding curcumin and garlic extracts to the diet of growing rabbits affected productive performance, carcass characteristics and antioxidant status. Sixty growing rabbits (APRI) were divided into five groups of 12 rabbits each at the age of six weeks. Five diets containing the same proportions of all nutrients were made. The first control diet (without any additives); curcumin (CE) or garlic extract (GE) at concentrations of 200 or 400 ppm were added to the other...
Rabia Bibi1, R. Muhammad Tariq2, Samir A.M. Abdelgaleil3 and Munawwer Rasheed1, 4*
...nt concentrations of the extracts were subjected to the toxic assessment against two species of stored grain pests (Sitophilus oryzae and Callosobruchus maculatus). Results obtained from experimental trail proved that the C. maculatus were susceptible with higher adult mortality than S. oryzae. The highest toxic effect was induced by dichloromethane obtained extract from A. taxiformis (DA) against C. maculatus (LC50 1.15 mg/cm2) after 24 h exposure and S. oryz...
Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*
...Geranium thunbergii (GT) extracts and liquid mineral (LM) in drinking water, on the growth performance, breast and thigh meat yield, digestive organ weights and meat quality characteristics in broilers. A total of 210 broilers were divided into the following treatment groups: (i) control, (ii) T1 (0.05% 4 MA: 1 GT + 0.01% LM), (iii) T2 (0.05% 4 MA: 1 GT + 0.05% LM), (iv) T3 (0.05% 4 MA: 1 GT + 0.1% LM), (v) T4 (0.1% 4 MA: 1 GT + 0.01% LM), (vi) T5 (0.1% 4 MA: ...
Ahmed Alazzouni1, Ashraf Al-Brakati2,*, Sherif Rabie1, Mohamed Gabry1 and Basmaa Hassan1
... curcumin, and green tea extracts against benzene chromasolv-induced leukaemia in rats and their ability to alleviate the histopathological alterations in the mesenteric lymph nodes (MLNs) and spleen. In this study 70 rats were divided into seven groups as follow: control, benzene (0.2 ml twice/week), Moringa oleifera (100 mg/kg), curcumin (300 mg/kg), green tea (350 mg/kg), combined green tea and curcumin, and cyclophosphamide (7.5 mg/kg) groups. All groups w...
Rasha Ali Taha Hamza1, Atef Saad Osheba1, Hassan Mohamed Sobhy2, Sahar Hussein Abdalla Hekal2*
...both water and ethanolic extracts of galangal and sumac extracts. Also, the effect of previous extracts on quality attributes of beef burger was evaluated. The ethanolic extracts of both galangal and sumac were shown to have significantly higher total phenolic and flavonoid contents than the aqueous extract, in particular 90% Ethanol Extract, where the 9...
Jiang Wu*
...perties of T. officinale extracts against foodborne pathogens were evaluated by agar diffusion method. Sub-inhibitory concentration (SIC) value, minimum inhibitory concentration (MIC) value and minimum bactericidal concentration (MBC) value were measured. The virulence gene expression of the four foodborne pathogens was analyzed by quantitative real-time PCR (qRT-PCR). A microplate reader was used to monitor cell growth at 600 nm with 1 h interval. Environment...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
... EDTA) and various plant extracts on the enzyme activity were also tested. Cellulase was further purified and characterized through chromatographic techniques and electrophoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa....
Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1
...rmulations and botanical extracts against C. quinquefasciatus larvae. Among the n-hexane extracts of 40 indigenous plant species collected from Soon Valley and surrounding salt range of Pakistan bioassayed against C. quinquefasciatus larvae, eighteen botanicals exhibited more than 50% larval mortality in 48 h exposure. The most effective botanical extracts were Maerua arenaria Forsk, Neriu...
Muhammad Ismail1,*, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1 and Umair Abbas1 and Riaz Hussain2
...ntrol. Insecticidal phytoextracts are biorational alternates to hazardous synthetic insecticides. This study evaluated the efficacy of aqueous extracts of neem (Azadirachta indica A. Juss), garlic (Allium sativum L.), ginger (Zingiber officinale Roscoe) and lime citrus (Citrus aurantifolia (Christm.) Swingle) on the fruits of five citrus cultivars (i.e. bitter orange (Citrus aurantium L.), grapefruit (Citrus paradisi Macfad)...
Asmaa Khamis1, Osama Abdalla1, Mohamed Hashem2, Noha Abdelnaeim1*
... of both medicinal plant extracts on rats intoxicated with carbon tetrachloride (CCl4). A total of 60 male albino rats were equally distributed in six groups. The first group received purified water and was kept as a control. The second and third groups were given oral PN and PM (500 mg/kg/day) for 31 days, respectively. The fourth group was intraperitoneally injected with CCl4 (2 ml/kg/day) on days 15 and 16 of the experiment. The fifth and sixth groups recei...
Iram Liaqat1*, Umaima Bibi2, Muhammad Arshad3 and Najma Arshad2*
...nd, H. officinalis plant extracts exhibited low free radical scavenging ability and antioxidant potential. Obtained knowledge via present study have been extended further for isolation of potential bioactive components with natural and inherent antimicrobial and antioxidant properties....
Oluwatoyin Adenike Fabiyi
...ato was evaluated. Crude extracts of M. indica bark was fractionated on silica-gel (120-150 mesh) using glass column. The resulting fractions were investigated in screenhouse and field trials. The fractions were equated with carbofuran a synthetic nematicide. Each experimental pot containing 40 kg of pasteurized soil was inoculated with 1000 eggs of M. incognita in the screenhouse, while 2500 eggs was introduced to the base of each tomato plant on the field. S...
Saima Mehar1 and Salma Javed2*
...the current study. Crude extracts were tested against Meloidogyne incognitain vitro conditions. The second stage juveniles (J2) were exposed at 24, 48 and 72 hours in different concentrations of plant extracts. During in-vitro condition inhibition of egg hatchability and J2 juveniles’ mortality varied according to the concentration of plant extract. WC=1 showed 100% mortality after 48 hrs of exposure at 1% with the let...
Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5
...activities of methanolic extracts of T. camphoratum leaves. The methanolic extract was quantitatively evaluated for alkaloids, flavonoids and terpenoids contents. The antibacterial activity of methanolic leaves extract of T. camphoratum was carried out against Gram-positive and Gram-negative bacterial strains through agar well diffusion assay (small wells were made with cork borer and 6μl of plant extract was added to the wells). The antioxidant activity of...
Muhammad Rizwan, Abu Bakar Muhammad Raza*, Muhammad Zeeshan Majeed and Muhammad Arshad
...would be the use of phytoextracts having insecticidal activity. The main objective of this study was to find out the toxic effect of various plant extracts against D. mangiferae. Three concentrations (i.e. 6.25, 12.5 and 25.0%) of leaf extracts of neem, Azadirachta indica A. Juss (Meliaceae), eucalyptus Eucalyptus camaldulensis Dehnh. (Myrtaceae), datura Datura stramonium L. (Solanaceae), ...
Haitham Al Masruri1, Rashid Al Zeidi2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Ahmed Al-Madani4, Al-Hassan Mohammed5*
...ifera leaves, seeds, and extracts are administered to animals for therapeutic and nutritional purposes. The effects of using M. oleifera and its extracts on animals’ production and health have shown beneficial effects in several studies. The roles of M. oleifera and its extracts on nutrient digestibility, rumen fermentation and enzyme activities, growth and reproductive performances,...
Ghalib Ayaz Kachelo1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Shahbaz Talib Sahi1, Nasir Ahmad Khan1, Akhtar Hameed2, Noor Muhammad1 and Muhammad Saqib Mushtaq1
... of fungicides and plant extracts against leaf spot disease of spinach caused by Alternaria alternata five plant extracts and five fungicides were evaluated under in vitro and field conditions by using complete randomized design (CRD) and randomized complete block design (RCBD). In plant extracts moringa (Moringa oleifera) showed minimum fungal growth (20.790) mm followed by ginger (Zingib...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...n bovine. However, Plant extracts has lately appeared as a low-cost, it considers a natural source of chemicals for preserving, also enhancing activity of sperm during different period storage. Current paper gives an apprise on the usage of garlic (Allium sativum) extract as an alternative additive for sperm preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as...
Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov*
...ith a protein present in extracts from cow placenta. The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.
Keywords | Bovine pregnancy-associated glycoprotein 1, Fusion protein, Recombinant antigen, Monoclonal antibodies, Placental antigens.
...
Muhammad Younas1*, Khalid Hussain1, Abdul Ghaffar1, Muhammad Atiq2, Niaz Hussain1, Wasim Abbas3, Muhammad Azeem Khan4, Muhammad Nadeem1, Muhammad Irshad1, Nasir Ahmad Khan2 and Muhammad Zubair5
...ome fungicides and plant extracts was determined for disease suppression on a susceptible variety of onion. During both years of study, eight accessions (Phulkara, Texas Early, Cylon, Sunset, Red Gystal, Rubi F1, Red Flame, ON-14133) exhibited resistant response with minimum percent disease index (PDI) which ranged between 5-10%, followed by Mirpurkhas, Nasarpuri, HON-1069, Pania, Rubi F2, GSL-132, Red Moon, PK-1032, F-1122, Red Imposta, Early Red, Desi Large ...
Oluwatoyin Adenike Fabiyi
...nd organic solvent crude extracts (40, 60, 80g/kg soil) in M. incognita infested lettuce and carrot plants as treatments. Results showed that lettuce and carrot plants grown in pots amended with the highest quantity of plant materials (800g) had commendatory vegetative growth all through the study weeks as against the plants treated with organic solvent extracts and the untreated control plants. Vegetative parameters differ ...
Riaz Hussain1*, Adnan Ihsan2, Azaz Ali Shah2, Najeeb Ullah2, Hamza Iftikhar3 and Ranra Jalal4
...t-align: justify;">Plant extracts appear as promising biorational alternatives to hazardous synthetic insecticides. This research was carried in a farmer’s field in Malam Jaba (Swat, KPK) during 2019-2020 Rabi season to evaluate the efficiency of some selected botanical extracts against the key insect pests of green pea (Pisum sativum L.) and their effect on yield/cost benefit ratio. The randomized complete block desig...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
...tube. The hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum, and their active ingredients including carvacrol and hypericin, were prepared, and then TiO2N.Ps. were prepared from them using isopropoxide. The antibiotic methicillin and calcium alginate pad was also loaded as control of nanoparticles embedded in the calcium alginate pad and then the restorative effect on the wound was studied. On days 0, 3, 7, 14, and 21, hematoxylin-eosin slides ...
Abdul Latif1, Shahid Sattar1, Fazal Maula2, Imtiaz Khan4*, Asim Iqbal3 and Said Hussain Shah1*
Fawziah M. Albarakaty*
...runus domestica L.) peel extracts on certain crucial аnimal microbes. Methodology: The antibacterial activity of plum (Prunus domestica L.) peel extracts in hоt and cold aqueous and ethanol extracts against specific medically significant pathogens isolated from cows and poultry farms were assessed. Additionally, the phytochemical composition of the aqueous and ethanol peel
Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2
...detected by DAS-ELISA in extracts from inoculated host leaves. The virus was transmitted by grafting on fig plants and sap inoculation on a very restricted host range of herbaceous plants where showing latent symptom. The virus with flexible rod particle ca. 650 nm long denoted FLV in fig trees in Egypt orchards. The viral Coat protein gene structure resembles that of members of the genus Trichovirusin the family Flexiviridae. In this study, the virus isolated...
Ayesha Saeed1*, Farzana Kishwar2, Ahsan Nazir3, Sharjeel Abid4, Muhammad Abiodullah5, and Danial Ali6
...er mask loaded with leaf extracts of MA and HRS as antibacterial agents in SA/PVA nanofibers through electrospinning method, which was just wet before its application. Leaf extracts were used at three concentration levels (0.5, 1, and 1.5 %). Scanning Electron Microscope (SEM) images confirmed the formation of optimized nanofibers with diameter range from ±49 to ±545 nm. It was observed that addition of leaf ex...
Shahid Iqbal, Muhammad Aslam Khan, Muhammad Atiq*, Nasir Ahmed Rajput, Muhammad Usman, Ahmad Nawaz, Ghalib Ayaz Kachelo, Azeem Akram and Hadeed Ahmad
...biocontrol, use of Phyto-extracts and nanotechnological approaches have been introduced to combat this disease. The synthetic fungicides are used to curb the disease incidence. Pathogen have developed resistance against various chemicals that are generally utilized to overcome this disease. Because of its antifungal potential against mango anthracnose, among these strategies, nanotechnology is a rapidly evolving discipline that is gaining the attention of rese...
Shahid Iqbal1,2, Zulha Javed1, Mushtaq Hussain Zahid3, Qurat ul Ane Gillani4 and Furhan Iqbal1,*
...ves effects of ethanolic extracts of leaves of Pongamia pinnata (L.) Pierre and Eucalyptus camaldulensis Dehnh on hematological parameters and biochemical components of blood serum of male albino mice. The extracts were administrated orally (100 mg/ ml solvent/ Kg body weight) for 15 days and it was observed that the complete blood count (CBC) remained unaffected while cholesterol (P = 0.03) and triglyceride (P = 0.008) leve...
Mehwish Saleem1* and Amina Atiq2
...0.01g, 0.025g, and 0.05g extracts of both aqueous and ethanol to create 0.01%, 0.025%, and 0.05% extract solutions, respectively. All of these extracts were then mixed with silver nitrate to create a 0.01M solution of silver nitrate nanoparticles. UV-VIS was done for the characterization of the nanoparticles. Various bacterial strains were successfully combatted by garlic nanoparticles’ strong antibacterial properties ...
Nosheen Jehajo*, Nasreen Memon, Mansoor Ali Shah and Naheed Shah
...gly recommended that oil extracts from seeds of neem and castor plants can be used to manage the stored grain pest up to the tolerable limits in integrated pest management. Moreover, the present study suggests further work on the efficacy of some other local plant extractions as an alternative to chemical pesticides....
Hafiz Husnain Nawaz1*, Ayesha Ahsan2, Amir Afzal1, Muhammad Ashraf Sumrah1, Muhammad Jan1, Kashif Ali1, Muhammad Arsalan1 and Rizwan Latif3
... content of oil and leaf extracts. Bioactive components included in plant extracts, such as tocopherol, chlorophyll, carotenoids, phenolics, flavonoids, and other scavenging components, make them more potent. Thus, the 50µg/ml concentration plant extract solution has the lowest radical scavenging percentage, whereas the 1000µg/ml concentration plant extract solution has the highest DPPH percentage 80%. The result...
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
...ounds, and natural plant extracts. The aim of this approach is to lower the population size of pests by reducing the natality rather than by increasing mortality. Male (20) and female (40) albino mice were orally administered testosterone at 25 mg/kg for 35 days. The males were allowed to mate with females to estimate the fertility index. After the end of treatment, mice were sacrificed, blood was collected for biochemical analysis, and sex organs were dissect...
Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8
... justify;">Crude solvent extracts of traditional medicinal plants have been used for thousands of years in different region of the globe for the treatment of various diseases. In developing countries, traditional medicines are used as source of primary health care. Keeping in view the importance of Camellia sinensis L., present investigation was aimed to evaluate the phytochemical and pharmacological potential of different morphological parts of C. sinensis L....
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
...t of different botanical extracts on the olfactory response of B. zonata and on the post-harvest quality parameters of banana was evaluated. Extracts of Moringa olifera, Mentha pipertia, Zingiber officinale, and Cinnamomum spp. were prepared in methanol, ethanol, and distilled water. The olfactory response of B. zonata was studied in Y-tube olfactometer and effect on the quality parameters (colour, taste, weight loss, pH, an...
Amal G. Abdelrahman1, Nabil A. Yassien1, Hussein M.H. Mohamed1, Khaled S. Tolba2, Heba H.S. Abdel-Naeem1*
... with a mixture of those extracts (5% kiwi + 5 % pineapple) in addition to the control group. All groups were kept in frozen storage at ‒18 °C for 3 months and subjected to proximate chemical analysis, measurement of deterioration criteria, and sensory quality. The results revealed that all enzyme-treated samples significantly increase protein content and significantly decrease the fat content, pH, and thiobarbituric acid value with a significant improve...
M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1
...ffect of medicinal plant extracts viz., marigold (Tagetes spp.), rosemary (Rosmarinus
officinalis L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000
juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments
reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the
S. Feroza1, A. G. Arijo1† and I. R. Zahid2
...000 eggs/bird. Ethanolic extracts of papaya and neem were applied to Group B and C, respectively while Group A was left untreated that served as control. The fecal egg count (FEC) was conducted on weekly basis. The pre-treatment values of FEC in all three groups found negative from day 0 to 14 after artificially inducing infection. On day 21, the FEC pretreatment values in group A, B and C were recorded as 1424.5, 1346.3 and 1806.4, respect...
A. M. Khan1,†, S. Naz1 and M. Abid2
...its powder and different extracts
including n-hexane, DMSO and water extract against naturally occurring soil nematode namely Meloidogyne
javanica anti fungal activity against Fusarium moniliforme and Rhizoctonia solani and fertilizing potential on okra
Abelmoschus esculentus. It was observed that the soil sample which was mixed with algal powder supported the
growth of the okra plant and proved that the alga und...
K. Taimoor1† and F. Shahina2
...ation were recorded from extracts of Matricaria lesiocarpa
which was 3532.5 at dose 3g/liter. Similarly minimum root-knot index (1.0) was recorded from extracts of fourteen
medicinal and aromatic plants at dose of 3.0g/liter and reproductive factor was also minimum 0.56 at dose of
3.0g/liter from extracts of Zatoria multiflora, Ferula oopoda and Matricar...
A.A. Tanimola and B. Fawole1
...trations of Aloe species extracts inhibited egg-hatching and second-stage juveniles mortality
was observed significantly when compared with water control. The AE of AKY leaves at 50,000 mg/kg was the
most effective in egg-hatching inhibited (95.4±1.7%), followed by AVR (94 ± 0.8%) and AST (88 ± 1.4%). Water
extracts of leaves of AKY, AVR and AST inhibited egg-hatching ...
I. Umar† and A. Mamman*
...g both crude and diluted extracts of fruit powder (5, 10, 15 and 20 ml) except the control,
which received only distilled water. Petri dishes were treated to the same concentrations of the extract in Petri
dishes containing 1000 second stage juveniles of M. javanica. Egg hatch inhibition and larval mortality was
observed over a period of 72 hrs. Sterilized loamy soil (4 kg) contained in 36 perforated plastic pots (30 cm dia....
O. A. Fabiyi
...cetone. The concentrated extracts were
subjected to chromatography on a silica gel packed column. The nematicidal activity of the fractions were
evaluated in the laboratory using a 4x4x3 factorial experiment, while the crude extracts were tested on
Meloidogyne incognita infecting Abelmoschus esculentus in the field. Three hundred juveniles were used for the
assessment. Acetone ...
T.S. Rizvi and F. Shahina†
... pulp, fruit and creeper extracts were evaluated against rootknot
nematodes (Meloidogyne spp.) at 28 ± 2 C in laboratory. Gas chromatograph interfaced to a Mass
spectrometer (GC-MS) results indicated that C. colocynthis contains various bioactive compounds. The mentholic
extracts of seed had the highest mortality (100%) at highest exposure time (72 hrs) while, the pulp and fruit
A. Khan†, Saifullah, M. Iqbal* and S. Hussain
...the application of crude extracts of Pleurotus species never recovered to life after
placing in simple water. P. citronopileatus was found more effective than other species and it killed 100%
nematodes after 24 hrs followed by fruiting body extracts of P. florida 99% and waste of P. ostreatus 77%.
...
W.M.A. El-Nagdi†, M.M.A. Youssef and M.G. Dawood*
...m melongena with aqueous extracts of garlic Allium sativum mashed clove and oil. The plant materials were
diluted with distilled water at concentrations of 2.5, 1.25 and 0.625% and drenched in each plot. Results showed that
the plant extracts showed nematicidal and nematode-hatching inhibitory activity reduced nematode criteria including
number of galls, egg-masses and hatched juveniles on...
Jax Vincent Gamulo, Maye Pearl Bolina, Jessica Serena Brion, Via Crishiela Nicole Dela Rosa, Roxanne Francesca Maglaya, Carl Lexter Tan, Aimee Caye Chang*
...e between tropical plant extracts and commercially available drugs posed a significant difference (P < 0.05), while ten (10) among the plant species differed in fasciolocidal activity (P < 0.05). Albendazole (62.5%) was the most used reference drug, along with Piperazine Citrate, and Triclabendazole, wherein ANOVA showed no significant difference in mortality time among them (P > 0.05). Notably, plant parts and extraction techniques used affected the ...
Rukhsana Anwar1*, Ammara Abd-Us-Sattar1, Afifa Noor2, Kanwal Ashiq1,3 and Shah Jahan4
...dy, 200 mg/kg BW of both extracts were orally administered once daily for 19 days to different groups of rats. CYP modulators were administered to respective groups for last 5 consecutive days. Real-time PCR analysis of hepatic CYP450 subtypes, CYP1A2, CYP2C9, and CYP3A4 was carried out. Moreover, phase II drug-metabolizing enzymes; GST-specific activity, total glutathione, superoxide dismutase, catalase, and malondialdehyde were determined in liver homogenate...
Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4
...tential of various plant extracts was evaluated using well diffusion and disc diffusion assay against various disease-causing microorganisms. In the case of well diffusion assay, the plant exhibited a maximum 16mm inhibition zone against B. subtilis a minimum 10 mm inhibition zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K. pneumoniae as compared to control. When tested on S. aureus, chloroform showed a 10 mm inhibit...
Mutia Rizkia Shaffira, Nurhayati, Dwi Desmiyeni Putri*
...red ginger and brotowali extracts in drinking water both single or in combined application improved on body weight gain, conversion ratio, mortality, carcass percentage, abdominal fat, and cooking loss significantly (p<0.05). Administration of a combination of red ginger and brotowali extract in drinking water resulted in the best body weight gain (1100.28 g/head), Feed conversion ratio (1.37), carcass percentage (83.58%), abdominal fat percentage (0.56%) a...
B. Archana and R. Saxena
...showed that out of these extracts O. sanctum found to be the most effective. In vivo experiment was conducted to study the effect of root extract of O. sanctum on M. incognita infecting French bean. Different concentrations viz., 10000 ppm (S), 5000 ppm ( S/2), 2500 ppm (S/4), 1250 ppm(S/8), 625 ppm (S/16) and 312.5 ppm (S/32) of aqueous extract of O. sanctum roots was applied by seed soaking and foliar spray methods....
O. A. Fabiyi, G. A. Olatunji and O. Atolani
...from the resulting crude extracts were further subjected to open column chromatography on silica gel (100-120 mesh grades) using glass column. The chromatographic fractions were tested in vitro along with their crudes and carbofuran on Meloidogyne incognita
Saleem Hussain1, Muhammad Tayyab1, Tauqir Anwar1, Talha Nazir2*, Muhammad Zeeshan Majeed3, Zohaib Asad2,4, Muhammad Adnan3 and Tajwar Alam5
...ppm) of methanolic plant extracts under controlled laboratory conditions, while three different concentrations (25, 50 and 75 g/L) of each botanical extract was tested in the field trial. Results showed a significant mortality and suppression of S. graminum individuals at 72 h after treatment in the laboratory and at 10th day post-treatment in the field. In laboratory bioassay, maximum aphid mortality was caused by the extracts<...
TAHIR MEHMOOD*, ANUM AFZAL, ABDUL KARIM, MUHAMMAD ASHRAF SHAHEEN &
HINA ARSHAD
...ty) of different solvent extracts, from Citrus limon (unripe and ripened) peels. In unripe peels the total flavonoid contents, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power ranged from 18.23-57.56 mg/g CE (catechin equivalent), 21.69-65.82%, 38.96-84.83% and 0.01-1.8 mg /mL, respectively. For ripened lemon peels all these indicators ranged from 10.53-47.88 mg/g CE, 37.99-81.40%, 42.96-95.93% and 0.07-1.47 mg /mL respecti...
SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2*
... sugar contents of apple extracts into ethanol. Different parameters like incubation time, temperature, pH and Inoculum size were optimized to get the maximum ethanol production that can be increased after further studies and proved to be a beneficial candidate for different applications. Maximum ethanol (13.9 %) was produced after inoculation was performed by S. cerevisiae (inoculum size 4%). The optimized conditions of apple e...
SAIMA AFTAB1, ATTIA HAMID1, MAHJBEEN2, JAVERIA TARIQ2, ZULQURNAIN ALI2, ASMA ZAFAR2*
... sugar contents of apple extracts into ethanol. Different parameters like incubation time, temperature, pH and Inoculum size were optimized to get the maximum ethanol production that can be increased after further studies and proved to be a beneficial candidate for different applications. Maximum ethanol (13.9 %) was produced after inoculation was performed by S. cerevisiae (inoculum size 4%). The optimized conditions of apple e...
SYED ALI RAZA1, MUHAMMAD WASEEM MUMTAZ2*, AYOUB RASHID CH1, ABDUL WAHEED1, JAMES WILLIAM3 & MUHAMMAD ARSHAD1
...rent hydroethanolic leaf extracts of Conocarpus lancifolius. Freeze drying assisted ultra-sonication was adopted for extract preparation. The results showed that highest total antioxidant power of 291 ± 0.50 mg ASE/g DE was exhibited by 60% ethanolic extract which was slightly lower than antioxidant power of BHA (309.16 ± 3.90 mg ASE/g DE). The β-carotene bleaching inhibition of 87.44 ± 1.20% was computed for 60% ethanolic leaf...
Arief1, Roni Pazla2*, Rizqan1, Novirman Jamarun2
...fiber, and nitrogen-free extracts (NFE), and the correlations between fat intake and milk fat contents, crude fat intake, and milk fat content and between NFE intake and milk lactose contents. The results showed that the treatment had no significant effect on production, fat content, milk lactose, intake, and digestibility of fat, crude fiber, and NFE but there was a relationship between fat intake with milk fat contents, crude fat intake with milk fat content...
Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3
...achta indica (A. indica) extracts on expression of NS3 and NS5A nonstructural proteins of HCV 3a genotype. Owing to the fact that HCV patients can develop HCC, plant extract has also been tested for cytotoxic activity. Colorimetric analysis was done to determine the vitality of HepG 2 cells for 24 h after treatment with methanolic seed extract and minimum inhibitory concentration was calculated. HepG2 cell were transfected stably to express nonstructural prote...
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
...er officinale methanolic extracts on eggs of Fasciola spp. to both positive (Albendazole) and negative (chlorine-free water) control groups in vitro. Microscopic examination of the treated Fasciola spp. eggs with ginger methanolic extract revealed that the various concentrations of Z. officinale methanolic extract used in our study were highly sensitive to it and that their activities differed noticeably from those of the positive (albendazole) and negative (c...
Hasanain Jihad Neamah
Usama Saleem1, Muhammad Asrar1*, Dilbar Hussain2, Saddam Hussain1, Abdul Ghaffar2, Hassan Usman1, Muhammad Imran1, Mubshar Saleem3 and Maryam Hamid1
...fectiveness of botanical extracts and synthetic insecticides in combating wheat aphids. Data regarding the aphid population was recorded 24, 48, 72, and 168 hours after the application of plant extracts and insecticides. Maximum mortality recorded after 168 hours was, 85.06% and 79.29% by Neem and Eucalyptus leaf extract followed by 72 hours 72.29% and 71.69%, 48 hours 63.35% and 62.45% and 24 hours 48.91% and 48.68% respect...
E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2
...phoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe....
Muhammad Afzal Naeem1*, Muhammad Hassaan Khan2, Tahmina Nazish3, Uzma Bashir4 and Midrarullah5
...and treated with aqueous extracts from various parts of G. hirsutum (root, stem and leaves) at concentration (0-100% v/v). Among the various test extracts of G. hirsutum, leaf extract were found to impart maximum inhibitory effect on the germination indices of the wheat (T. aestivum) followed by the stem extracts, as the highest concentration of ACWE (i.e. 100%) caused the reduction of 45....
Riaz Hussain2*, Adnan Ihsan1*, Waqar Khan1, Shiraz Malik1, Hamza Jameel1, Hidayat Ullah3, Shahab Ahmad4, Jawad Anwar5 and Murad Ali6
...fficacy of various plant extracts viz., (Green Chili (Capsicum spp.), Garlic Fresh Cloves (A. sativum), Chinaberry Fresh leaves (M. azedarach), Eucalpytus leaves against pea aphid Acyrthosiphon pisum. The research experiment was design randomize complete block (RCBD) with three replications and two sprays were applied at 14 days interval. After 7 and 14 days of 1st and 2nd application the minimum means density of aphids were recorded in plot treated with Eucal...
Saima Mehar1, Amtul Sami2 and Erum Iqbal3*
...t was determined for the extracts of Cyminum spp. (Cuminum cyminum and Nigella sativa) in methanol, chloroform, ether, butanol, and hexane on egg hatching and mortality of the juveniles of root-knot nematode Meloidogyne javanica. After 72 hours, C. cyminum (MeOH) demonstrated the maximum nematicidal activity in egg hatching (15%±2.10) of M. javanica, followed by N. sativa (Ether), 22.33±6.86%, at the lowest administered dose of 0.25 % concentrati...
Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Summer, Ali Haidar Gormani, Saira Nawaz and Ayesha Muzamil
... potentials of hot water extracts of mulberry leaves (mulberry tea) of two species i.e., Morus alba and Morus nigra. Mice were fed orally with mulberry leaves extract (200mg/kg, 100mg/kg and 50mg/kg) mixed in distilled water. Mice paw edema was induced by carrageenan injection while formalin was injected to mice paw for induction of pain. These extracts significantly decreased edema in mice paw. The level of TLC and RBCs was...
Indri Juliyarsi1*, Sri Melia1, Rizki Dwi Setiawan1, Deni Novia1, Lailatul Anshor2, Tomi Candra2
...ferent levels of rosella extracts (0, 0.2, 0.4, and 0.6%). The addition of rosella calyx extract led to a significant difference (P<0.05) in color (redness), antioxidant activity, and color sensory attributes, but did not affect the moisture content, aroma, and flavor (P>0.05). The greater the amount of rosella extract added, the higher the a* value of the color attribute (redness), which gives a deeper red color to the whey edible film. Increasing the c...
Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1
...hat almost all the plant extracts showed potential antibacterial, insecticidal and antifungal activities against different. The research has focused on the potential therapeutic efficacy of AliumSativum, NigelaSativa, and Plantago Ovata in the treatment of microbial infections due to their significant antibacterial activity. Additionally, Different fractions of the A. sativum, P. ovata, N. sativa were prepared anad analyzed for antibacterial, insecticidal and ...
Dina A. Yousif, Mohamed M.T. Emara, Marwa R.S. Abdallah*
...r officinale crude water extracts might be utilized to increase the sensory acceptability of old mutton, particularly the flavor. Three groups of mutton flesh were used in the study; one group had a plain marinade, while the other two groups received 5% fresh water extracts of either Mentha piperita folium or Zingiber officinale added to the plain marinade. All samples were marinated for 24 h at 4 °C before being cooked ...
Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova*
...positories obtained from extracts for intrauterine administration, with 20% and 40% content of C. leucocladum. Previously, phytopreparations were tested for antimicrobial (diffusion in agar by serial double dilutions) and antiradical activity (comparison with Butylhydroxyanisole (BHA), as well as for toxicity and the ability to cause irritation or an allergic reaction from the mucous membranes. In cows that used a suppository with a 40% content of C. leucoclad...
Abdur Rauf1*, Farooq Jan1, Muhammad Qayash2, Zia-ul-Islam3, Rizwanullah1, Ikramullah Khan1, Muhammad Shuaib4, Muhammad Khalid5 and Samrin Gul6
... various ethanolic crude extracts of H. eichwaldii revealed the highest potential (15.82±0.40), (18.12±0.56), and (17.41±0.51) against S. aureus, a moderate activity (13.31±0.64), (15.43±0.52), (14.35±0.64), (11.34±0.50), (13.47±0.55), (14.21±0.67), (9.53±0.51), (15.32±0.53), and (2.15±0.65) against E. coli, B. subtalus, and M. leutues. A 15mg/ml crude extract of H. eichwaldii ...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...ning using ethyl acetate extracts also showed significant ZI in the range of 06 – 14.0 mm (P ≤ 0.05) against these pathogens thus confirming their bioactive potential. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of screened Actinomycetes was in the range of 1.3 - 3.5 mgmL-1 and 1.9 – 4.0 mgmL-1 of bacterial biomass, respectively. Three isolates, S. monticola, S. septentrionalis and S. polaris showed 10...
Sherein H. Mohamed1, Soad El Naggar2*, Ayman A. Hassan3, Mohamed A.M. Mousa3, Mohamed M. Basyony3, Mohamed F. Sadek3, Mohamed A. A. Ahmed4, Saadia M. Hashem5
...influence of five herbal extracts in rabbit diets on productive performance, digestibility, cecal fermentation, antioxidant enzyme activities, immunity and meat quality. One hundred twenty six male growing New Zealand White rabbits aged eight weeks old with an average body weight of 675±47.95 g were divided into six equal groups 21 rabbits each, control diet group, C fed the basal diet and the other five groups (GE, ClE, ThE, TE, and CE) fed on basal di...
Mahmoud Mohamed Ahmed Youssef and Wafaa Mohamed Abd - El-Hameed El-Nagdi
...itro conditions, aqueous extracts of Khella (Ammi majus) mashed fresh leaves and flowers at concentrations of 2.5, 5.0 and 10.0 % were bioassayed against root-knot nematode, Meloidogyne incognita second stage juveniles (J2s). The obtained results revealed that the tested extracts at 10% caused 100% nematode mortality at 72 h of exposure followed by the other concentrations with the percentages of net mortality ranging from 4...
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
...leaves, roots and floral extracts i.e. methanol,
chloroform, and n-hexane of Ranunculus laetus (R. laetus) were tested to
find out their bioactivities. The yield obtained from the methanolic extracts
was 15.12%, 20.5% and 31.3%, while that of chloroform extracts 4.15%,
16.15% and 29.6% and that of n-hexane extract 4.52%, 10.55% and
SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN
...edicinal plants or their extracts orally taken by the
marginal communities. Primary and secondary metabolites of Misopates
orontium (L.) belongs to Plantaginaceae family were screened out and
tested for therapeutic values through in-vitro biological assays.
Quantitative analysis was done on powder of Misopates orontium by
using standard methods for estimating the primary and secondary
meta...
Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1
...aled that the used juice extracts were variously bacteriostatic and bactericidal against the examined bacteria depending on concentration. Phytochemicals analysis indicated the presence of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) and MBC/MIC ratios (2.0-4.0). Furthermore, a time killing curve of e...
Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Farooq Bhatti, Muhammad Summer and Ali Haidar Gormani
...kaloids. Mulberry leaves extracts have also shown significant pharmacological activities including anti-inflammatory, antioxidant, antibacterial, anticancer, anti-diabetic and antidepressant activities. Further studies should explore the mulberry leaves potentials as these plants have got the attention of both the pharmacological and commercial industries.
Novelty Statement | The mulberry leaves posse...
Imtiaz Khan1*, Abdullah1, Muhammad Ibrahim1, Muhammad Ishfaq Khan1, Saima Hashim1, Shomayela Afzal2 and Khalid Nawab3
...s) and allelopathic weed extracts (Asphodelus tenuifolius Cav., Convolvulus arvensis L. and Lathyrus sp.) compared with control treatment. The study parameters include; weed density (m-2) before and after application, fresh and dry weight (m-2) of A. tenuifolius Cav., plant height (cm), number of nodes and branches plant-1, leaf area index (LAI), number of pods plant-1, number of grains pod-1, 500 seed weight (g), biological yield (kgha-1), grain yield (kgha-1...
Afsheen Khattak1, Shahida Naveed1*, Naila Khalid1, Inayat Ullah Khan2 and Tamana Bakht3
...d, powdered, and aqueous extracts were prepared at room temperature. Randomized Complete Design (RCD) with four treatments and one control were used in the experiment. Data was recorded for seed germination rate (%), shoot length (cm), radical length (cm), fresh biomass weight (g), dry weight (g), and moisture content (%) of wheat and maize seedling. Result revealed that V. encelioides extracts significantly affected all the...
Fatemeh Beheshti1, Maliheh Safavi2, Mohammad Reza Akbari Eidgahi1,3, Parviz Kokhaei4, Mehdi Vazirian5 and Ali Akbar Shabani1,3*
...y of organic and aqueous extracts of I. purpurea leaves was assessed. Further, the ethanol and chloroform extracts of I. purpurea leaves were analyzed using gas chromatography-mass spectrometry (GC-MS). Concentration-dependent scavenging of 2, 2-diphenyl-2-picryl hydrazil (DPPH) was seen across all three extract types (ethanol, water, and chloroform) with the IC50 values of 42.30 ± 2.35, 54.11 ± 3.62, and 85.71...
Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7
...on method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxidant activity in O. basilicum whereas, in S. asoca and M. koenigii 20% aqueous showed maximum activity. Total phenolic con...
Sabi Ur Rehman1*, Laiba Arshad1, Saman Ali1, Shazma Massey2, Safeer Khan3, Abdul Samad4 and Fazal-Ur-Rehman5
...cles utilizing botanical extracts. Silver nanoparticles are particularly noteworthy due to their potent antibacterial properties and relevance in nanomedicine. This study presents a green synthesis method using Sarcococca saligna leaf extracts to successfully produce both silver and gold nanoparticles. The formation and stability of the nanoparticles were verified through a change in physical colour and characterized using U...
Dan Song1,2, Ming-Juan Ge3, Jie Li1,2, Yi Jiang1, Xiu-Mei Kong1, Jiao-Jiao Xu1, Xu Ji1,2, Rui-Xin Shi1 and Qin Zhao1,2*
...ree G. straminea ethanol extracts dose groups (10, 5 and 2.5 g/kg respectively + hypoxia), administered intragastrically once a day for 14 consecutive days. After that, multiple hypoxia experiments were conducted including soda lime normobaric hypoxia test, sodium nitrite poisoning test, isoproterenol poisoning test, and acute cerebral ischemic hypoxia test. Subsequently, the content of superoxide dismutase (SOD), malondialdehyde (MDA), and activity of total a...
Rana Mahmood Ahmad*, Orooba Mohammed Maeed Ibrahim
...alities. Medicinal plant extracts are non-toxic, safe, and have no harmful side effects. Available in abundance, researchers have turned their attention to using these plants as anti-inflammatory. This study was designed to highlight and compare the effects of Syzygium aromaticum extracts oil and Piroxicam on inflammation circumstances. Extraction of clove bud with petroleum ether revealed a bright yellow extract with a typi...
Mahdyl , A.M.M.; Hafezl , M.A.; EL-Dougdoug2, Kh. A.; Fawzyl , R.N. and Shahwanl , Eman S.M.•
...body specific CMV. Water extracts of Mirabilis jalapa, Clerodendrum inerme and Kombucha as antiviral to control the virus infection and detection systemic acquired resistance (SAR) Were Studied. SAR was detected by determination of salicylic acid (SA) resulted induction of protein related to biotic inducers, virus concentration and disease severity (DS). The obtained results from quantification of total SA in induced tomato plants (after 7 days of spraying ind...
Alhudaib, Khalid
...till used. 8-10gl of TNA extracts were mixed with Igl random hexamer primer, (Boehringer Mannheim, GbmH) (0.5 g/gl). RT-PCR assay of leaves extracts of infected fig accession using specific primers gave positive results and non with FLMaV-2. Mixed infection of FLMaV-1 and FMV were found. To our knowledge this is the first record and identification of FLMaV-1 and FMV in Saudi Arabia. Further studies are needed to investigate ...
Xiaojun Li
...t was found that acetone extracts of Salviami ltiorrhiza Bunge (SIB) represented by cryptotanshinone and dihydrotanshinone had lethal effect on diamondback moth larvae, and the correlation coefficient was 0.972. Through further antifeedant test, it was found that diamondback moth larvae had obvious antifeedant behavior to acetone extracts of SIB represented by cryptotanshinone and dihydrotanshinone, and the correlation coeff...
Tabinda Nowsheen1, Sayed Wadood Ali Shah2, Ali Hazrat1*, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar4 and Muhammad Ajmal Khan3
...ay, the crude methanolic extracts showed significant inhibition against all tested bacterial strains at25, 50 and 100 µg/ml. The methanolic crude extract of A. maritime various parts showed MIC of 37.5µg/ml for S. aureus which is gram-positive bacteria followed by 75µg/ml for P. aeruginosa, (gram negative), and B. subtilis (gram positive) that is nearly similar to the activity of ciprofloxacin (standard). The methanolic leaves extract of A. m...
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim,
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
Nasir Shah1,4, Muhammad Ibrahim1*, Zarnosh Habib2, Kalsoom3 and Zahir Shah4
...peach fruit fly to plant extracts having insecticidal properties. Extract of three native plant species (Azadirachta indica, Zataria multiflora, Achillea santolina) and their various concentration (2, 1, and 0.5%) were used for the purpose. Firstly, the artificial diet of fruit flies was subjected to these treatments, while on the other hand chikoo fruits which were used for flies to settle on, were dipped in same concentrations of these plant
Muhammad Akram1, Anirban Mandal2*, Mehwish Iqbal3, Arindam Mukherjee4, Ritika Bandyopadhyay5, Surendar Rangasamy6, Rida Zainab1, Muhammad Talha Khalil1, Pragnesh Parmar7 and Umme Laila1
...ional antibiotics. Plant extracts can be good alternative in day-to-day illness instead of antibiotics misuse. Complementary and alternative medicines are good option for treatment of routine ailments. Complexes and constituents with antiviral activity exists in numerous plants, for instance, rutin, a flavonoid glycoside generally found in a range of botanicals, is efficient against herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), and ...
Noor A. Namaa*, Hayder A.N. Al-Zamely
...ificantly in the Ginseng extracts and Ginseng NPs groups. However, there was no discernible difference in the values of the Ginseng extract or Ginseng NP groups. When compared to the control group, the Ginseng extract and Ginseng NP groups showed increased mitochondrial expression of the SDHA protein. Comparison of the values of the therapeutic groups showed that the Ginseng NP group had higher values than the Ginseng extract group. In conclusion, Western blot...
SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2
..., methanolic and aqueous extracts of Aloe vera L. against six bacterial strains (Staphylococcus aureus; Streptococcus pyogenes; Shigella sonnei; Escherichia coli (1) (4C11); Escherichia coli (2) (ATCC 13048) and Neisseria gonorrhoeae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their antibacterial activities were associated with typical chemical like gentamicine (antibiotic...
ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1
...idal potential and their extracts have anthelmintic activity against Haemonchosis contortus....
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...e aqueous and methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 μl (1250 μg)...
UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR
...ntioxidant potentials of extracts were determined in terms of total phenolics, total flavonoids and ascorbic acid contents. In addition, radicals (DPPH• & ABTS•+) scavenging potentials were also determined. Results revealed that drying methods have no significant influence on recovery of antioxidants from sample matrix. However, methanolic extracts exhibited highest antioxidant potential that confirmed superior...
MUHAMMAD NADEEM1, MUHAMMAD WASEEM MUMTAZ1&MUHAMMAD DANISH1
...l of hydroethanolic leaf extracts of Vitexnegundo. Freeze drying assisted ultrasonicated extraction revealed highest level of total phenolic contents (258.29±2.52 mgGAE/g DE) and total flavonoid content (155.91±1.75 mgRE/g DE) in 60% hydro-ethanolic V. negundo leaf extract. Furthermore, total antioxidant power (254.35±1.56 mg AAE/g DE) and antidiabetic effectbased on α-amylase inhibitory assay (IC50=42.91±1.24 μg/mL) was al...
Olaolu Fadeyi1,2, Oluwatoyin Fabiyi3*, Tesleem Bello4 and Gabriel Olatunji5
...e use of different plant extracts with known pesticidal properties. This current research was conducted to determine the nematicidal status of Anacardium occidental fractions. Analysis of ethanol extract of A. occidentale using gas chromatography spectrometry revealed the presence of several phyto constituents such as phenol, triterpenoids, flavonoids and other bio-active compounds. The array of fatty acid esters revealed in the GCMS result of the fractions es...
Bilal Saeed Khan1*, Muhammad Aneeb Shahzad1, Muhammad Irfan Ashraf2, Zahid Mahmood Sarwar3, Muhammad Farooq4 and Awias Rasool5
...pellency of leaf aqueous extracts i.e., Melia azedarach (Bakain), Nicotiana tabacum L. (Tobacco), Eucalyptus globulus Labill (Eucalyptus), Moringa oleifera L. (Moringa) and Mentha piperita L. (Peppermint) against two major sucking pests i.e. aphid (Sitobion avenae F.) and two-spotted spider mites (Tetranychus urticae Koch) and their natural enemies such as ladybird beetle (Coccinella septempunctata Linnaeus) and predatory mite (Amblyseius andersoni Chant). The...
Tianhoun Denté Fidèle1,2*, Meda Nãg-Tiéro Roland2, Zabré Geneviève3, Koama Benjamin2, Kaboré Adama1, Tamboura H. Hamidou1, Bélem Adrien Marie Gaston4
...aqueous and hydroacetone extracts of Combretum micranthum leaves on the parasite Haemonchus contortus. Following phytochemical assay, five increasing concentrations (0.6; 1.2; 2.4; 4.8 and 9.6 mg/ml) of the two extracts were prepared and tested for egg hatching and larval development in the presence of positive (Albendazole) and negative (PBS) controls. Aqueous and hydroacetonic extracts o...
Iqtidar Hussain1*, Muhammad Inam Ullah Qaisrani1, Abdul Aziz Khakwani1, Zuhair Hasnian2, Umar Khitab Saddozai1, Muhammad Naeem4, Hadia Gul5 and Moneeza Abbass3
...ng effects of parthenium extracts at low concentrations have also been reported in certain crops. A laboratory experiment was carried out to evaluate the germination and germination indices of maize with estimation of maize growth infested with parthenium allelopathy. This trial was conducted Completely Randomized Design with 3 replications. Parthenium dry powder (5, 10, 15, and 20 %) mixed with soil produced variable responses regarding seed germination and s...
Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1
...ctively. In case of stem extracts, CS exhibited strongest antibacterial activity against E. coli, S. aurous and S. flexneri with inhibition zone of 24±0.23, 22±0.67 and 20±0.32 mm respectively while the same fraction showed maximum inhibition of linear growth (22±0.76 and 19±0.66 mm) of Candida albicans and A. niger, respectively. We conclude that V. negundo (L.) has potential antimicrobial and antifungal capacities which mak...
Arshad Javaid1* and Iqra Haider Khan
...t and flower
extracts (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0%) of the weed was checked on germination
and growth of the target weed. Leaf and stem extracts showed the best herbicidal activity
and a 2.5% extract of the each plant part completely inhibited germination of parthenium
seeds. The lowest extract concentration (0.5%) of leaf and stem reduced germination by 56
...
Rahamdad Khan1, Abdul Basit, Syed Majid Rasheed* and Syed Salim Shah
...tor B consisted of water extracts from root, stem and leaf of invasive
weeds; Parthenium hysterophorus and Xanthium strumarium. Distilled water was used as
control for comparison. Data were recorded for germination (%), seedlings length (cm),
shoot weight (g) and root weight (g). It was evident from the results that maximum
germination (100 %), Seedling length (7.6 cm), shoot weight (164.88 g) and root weight
Nimra Javaid1, Mukhtar Hussain Shah1, Iqra Haider Khan2, *Arshad Javaid2 and Syed Muhammad Waleed1
...6, 8 and 10%) of aqueous extracts of various parts of the tested weed were used in laboratory bioassays. ANOVA revealed the significant effect of plant part bioassay and extract concentration on germination as well as various root and shoot growth parameters. Leaf extract was found as the most herbicidal where a 10% extract completely inhibited the germination and growth of parthenium. Root and inflorescence extracts were al...
Muhammad Ather Nadeem1, Bilal Ahmad Khan 1, Sadia Afzal2, Muhammad Azim
Khan3, Tasawer Abbas4, Muhammad Mansoor Javaid1, Muhammad Mohsin Amin1,
Naila Farooq5 Amir Aziz5
...n system. Use of aqueous extracts of crops is getting scientific
attention as eco-friendly alternative to synthetic herbicides especially under scenario of fast
increasing herbicide resistance in weeds. To investigate the herbicidal potential of aqueous
extract of winter crop Carthamus tinctorius L. (safflower) on summer weed Oryza punctata
L. (red rice) seed emergence and initial seedling growth, a study was pla...
Muhammad Ather Nadeem1, Bilal Ahmad Khan*1, Sadia Afzal2, Ahsan Aziz1,
Rizwan Maqbool3, Muhammad Mohsin Amin1, Amir Aziz4, Amjed Ali1, Muhammad
Adnan1, Durrishahwar5
...e
of aqueous extracts of leaves, stem and fruit of Carthamus tinctorius L. at different
concentrations(0, 0.25, 0.50, 1, 2, 4, and 8%)on Echinochloa cruss-galli L. Results revealed
that 8%concentration of aqueous extracts of all tested parts of C. tinctorius was most
phytotoxic against germination as well growth of E. cruss-galli. Maximum mean emergence
time (5.60 d...
Muhammad Ather Nadeem1, Bilal Ahmad Khan1, Sadia Afzal2, Hasnain Abbas3, Muhammad Khuram Dar3, Muhammad Ehsan Safdar1, Ishtiaq Hassan4, Muhammad Asif1, Muhammad Adnan1, Amir Aziz5
... as control. The aqueous extracts of leaves, stem and flower of P. somniferum significantly inhibited the emergence, seedling growth as well as root length (cm), shoot length (cm), fresh weight (g) and dry weight (g) of O. punctata. Maximum mean emergence time (9.18 days) and minimum shoot length (1.13 cm) emergence index (0.89) and emergence percentage (6.67%) was observed under fruit extract at 8% concentration. P. somniferum aqueous extract of stem at 8% co...
Syeda Fakehha Naqvi1, Iqra Haider Khan1 and Arshad Javaid1
... Studies have shown that extracts of this weed possess antioxidant, antimicrobial, larvicidal and nematicidal activities. In the present study, n-hexane soluble fraction of methanolic stem extract of this weed was subjected to GC-MS analysis to identify various biologically active constituents. There were 28 compounds in this fraction. Major compounds included oleic acid (16.55%), palmitic acid (11.22%), β-sitosterol (9.63%), hexadecanoic acid (7.71%) and...
Muhammad Ather Nadeem1, Bilal Ahmad Khan*1, Saima Anwar2, Hasnain Abbas3, Muhammad Yasin1, Rizwan Maqbool3, Muhammad Mohsin Amin1, Amir Aziz5, Muhammad Sikander Hayyat1, Muhammad Shozib Javed5
...ent comprised of aqueous extracts of leaf, roots and fruits applied at 0.25, 0.5, 1, 2, 4 and 8% (w/v) along with a control (0%). Data regarding seed emergence, emergence index, emergence percentage, root length, shoot length and seedling dry weight were recorded. All the tested concentrations of all the parts of S. oleraceus significantly inhibited the seed emergence, emergence index, emergence percentage (%), growth and delayed mean emergence time and time t...
Muhammad Ather Nadeem1 , Bilal Ahmad Khan*1, Saima Anwar2, Ahsan Aziz1, Rizwan Maqbool3, Muhammad Kashif Sohail4, Muhammad Ehsan Safdar1, Muhammad Mansoor Javaid1, Muhammad Mohsin Amin1, Muhammad Kashif Iqbal1, Amir Aziz4
...>weed.
...
Farrukh Avais1, Anser Ali1, Hamza Javed2, Muhammad Saleem3, Tehreem Tahir4,
Muhammad Rafiq5,*
...ing 2018-2019 to isolate extracts from selected
plants (Garlic and Peppermint) to explore their tyrosinase inhibition activity. Tyrosinase is
a key enzyme of melanogenesis which determines the mammalian skin, hair and eye
colour. Hyper-pigmentation leads to various skin disorders like melasma, sunspots, age
spots and freckles. Moreover, abnormal skin pigmentation is a serious aesthetic concern
which l...
Barkatullah1, Sumayya Noreen1.Khushnood Ur Rehman1, Zahid Ali Butt2, Tabassum
Yaseen3, Kamran Akbar3, and Salma Noreen3
...e allelopathic effect of extracts is
directly related to the duration of soaking i.e. 48 h extracts were more effective than 24 h.
among all parts the highest activity was shown by leaves followed by fruit, bark, Rainwater,
and then mulching. From the above experiment, it is strongly suggested that the tested
portions of Z. mauritania have significant negative allelopathic effe...
Shabnam Javed1, Amna Shoaib2 and Zaid Mehmood1
...medicinal flora or their extracts taken orally by the trivial communities. In the
current study, different proximate parameters like carbohydrate, ash, protein, moisture
content and fat, along with carbon, hydrogen, nitrogen and sulphur were analyzed in whole
plant of S. tomentosa. The results revealed the occurrence of considerable proportion of
carbohydrates (52%) and protein (23.80%). Moisture, fat and ash con...
Shabnam Javed1 and Amna Shoaib2
...mentosa using methanolic extracts and fractions (n-hexane, dichloromethane, ethyl
acetate and water) against three cancer cell lines (lung A-549, hepatocellular HepG2and
urinary bladder EI-138). Cytotoxic assays were carried out with five concentrations (0.05,
0.01, 0.05, 0.1 and 0.5 mg mL-1) of methanolic extract and its subfractions through MTT
assay [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromid...
Muhammad Sikander Hayyat1*, Muhammad Ehsan Safdar1, Muhammad Mansoor
Javaid1
...irst experiment, aqueous extracts from various
plant parts of red sprangletop (stem, root, leaves, flower and entire plant) at their 5% (w/v)
concentration were applied to germinating rice seeds. In second experiment, soildecomposed
red sprangletop plant residues of variable concentrations (2, 4 and 6% w/w)
were used as germination media for rice. Among plant parts, red sprangletop leaves showed
maxim...
Muhammad Ather Nadeem1, Bilal Ahmad Khan1*, Saima Anwar2, Rizwan Maqbool3, Mohsin Amin1, Amir Aziz4, Iram Batool3, Athar Mahmood3, Abdul Rehman1, Aafaq Ali5, Aneela Nijabat5
...n system. Use of aqueous extracts of weed is getting scientific attention as eco-friendly alternative to chemical herbicides especially under scenario of fast increasing herbicide resistance in weeds. To investigate the herbicidal potential of aqueous extract of winter weed of Sonchus oleraceus L. (sow thistle) on summer weed Oryza punctata L. (red rice) seed emergence and initial seedling growth, the present study was planned. In this study, seeds of O. punct...
Muhammad Ather Nadeem1, Bilal Ahmad Khan1* Sadia Afzal2, Rizwan Maqbool3, Hasnain Waheed1, Aneela Nijabat4, Muhammad Ikram5, Amir Aziz6, Muhammad Adnan1, Qaisar Mehmood7, Hasnain Umer3
...mong them use of aqueous extracts of crops is an imperative method. In this method allelochemicals present in crop plant help in suppressing or inhibiting weeds. Therefore, this study was planned to investigate the allopathic potential of aqueous extract of winter crop Papaver somniferum (opium) on summer weed Echinochloa cruss-galli (barnyard grass. The experiment comprised of seven concentrations (0, 0.25, 0.50, 1, 2, 4, and 8%) of different plant parts i.e....
Eman Ali Hussein1*, Majdy Faisal Majeed2
...al gavages of TFG herbal extracts (0.15 g/kg BW) in addition to being kept on an identical experimental protocol to that of the rats in groups 1 and 2, respectively. While group 5’s diabetic rats that received orally gavages 3.5 mg/kg of glucophage XR treatment served as positive controls. Treatment with TFG of groups 3 and 4 recorded significantly (p≥0.05) enhanced of renal functions during blood urea nitrogen (BUN), creatine (CT), and urea (UR) as w...
MUHAMMAD AZIM KHAN,Khan Bahadar Marwat
Munesh Kumar , Siiolyne Siangshai
Muhammad Shahid, Bashir Ahmad, Riaz Ahmad Khattak , Gul Hassan , Haroon Khan
Muhammad Numan,Shabeer Jan,Muhammad Luqman,Zafar Iqbal,MUDASSAR IQBAL,Saiqa Jabeen,Muhammad Amin,Sartaj Alam,Sana Irshad Khan
Yoshiharu Fujii,RAZA ULLAH,Zahir Ahmad Zahir,Qamar-uz-Zaman,Sairah Syed,Abdul Khaliq,Misbah-ul-Islam,Zubair Aslam,Muhammad Zahid Ihsan
Sairah Syed,EJAZ AHMAD KHAN,IQTIDAR HUSSAIN,ASGHAR ALI KHAN,MUHAMMAD MANSOOR
Muhammad Nabeel,Ali Ahsan Bajwa,Sehrish Sadia,Wahaj Nafees,SHAHIDA KHALID
Sobiya Shafique, Rukhsana Bajwa, Arshad Javaid and Shazia Shafique
SHABNAM JAVED,Amna Shoaib,Arshad Javaid
Ejaz Ahmad Khan, M. Ayyaz Khan, H. Khalil Ahmad , Fateh Ullah Khan
Salik Nawaz Khan,SHUMAILA FAROOQ,SUMERA NAZ,GHULAM MOHY UD DIN
UZMA BASHIR,Amna Ali,MUHAMMAD SALEEM HAIDER,Naureen Akhtar
SAIRA BANARAS,SHEIKH MUHAMMAD IQBAL,Arshad Javaid
RUIDAR ALI SHAH,IMTIAZ ALI KHAN
Zahid Hussain,SAJJAD AHMAD,ANEES AMIN,MUHAMMAD NAEEM,HUSSAIN ALI,GUL HASSAN
ABID SUHAIL,MUHAMMAD SALMAN,FAWAD AHMAD,FAQIR GUL,MIDRAR ULLAH,MUHAMMAD ANWAR KHAN
Z.A.CHEEMA,M.MUBEEN,A.KHALIQ
Irshad Ur Rehman,SAEED ULLAH KHATTAK,Muhammad Kamran,Jamshaid Ahmad,Abid Ali Khan,Ghosia Lutfullah
SAEED UL HAQ,MIAN INYATULLAH,IJAZ AHMAD KHAN,MAID ZAMAN,FAZAL SAID,SAJJAD AHMAD
MUHAMMAD AZIM KHAN,Subhan Uddin,MUHAMMAD ILYAS,Rahamdad Khan,Salim Shah,SAJJAD ALI
EJAZ AHMAD KHAN,Haji Khalil Ahmad,FATEH ULLAH KHAN,Muhammad Ayaz Khan,HAJI HIMYAT ULLAH KHAN
SHUMAILA FAROOQ,MAKSHOOF ATHAR,Salik Nawaz Khan,SUMERA NAZ
Muhammad Azim Khan, Khan Bahadar Marwat, Gul Hassan , Z. Hussain
Arshad Iqbal,ASIM MUHAMMAD,Zia Ur Rahman,Irum Noreen,Fazl-e-Rabbi,Zamarud Shah
Irum Mukhtar,Sobia Mushtaq,Amna Ali,Ibatsam Khokhar,MUHAMMAD SALEEM HAIDER,SHABNAM JAVED
Wirat Phuwiwat , Watcharin Wichittrakarn , Chamroon Laosinwattana , Montinee Teerarak
Mohammad Javad Seghatoleslami , Gholam Reza Mousavi , Hossein Akbari
Muhammad Kamran, Ali Raza, Qasim Ali, Hafiz Haider Ali and Muhammad Sohaib Chattha
Asim Iqbal, Amjad Usman, Maqsood shah and Syed Sartaj Alam
Muhammad Imran , Azhar Mehmood, Tasawer Abbas, Ali Zohaib, Naila Farooq, Irfan Rasool Nasir and Shahzad Ahmad Junaid
Shahid Sattar, Bisma Mumtaz, Bashir Ahmad and Zafar Iqbal
Chika Bright Ikele1*, Ugwu Venita Uju1, Chioma Faith Ikele2
.../ml (A-E) (for all plant extracts used) in a 96 micro-wells chambers, with 3 sub-replicates of approximately 160 theronts. Mortality occurred in all groups except in the control group during the exposure period. 24-h theront mortality was highest in bitter leaf extracts and least in cashew leaf extract. Mortality was also dependent on extract concentration and extraction solvent. The two highest mortalities of 54.2% and 53.1...
Marah Salim Hameed1, Raad Mahmood Hussein Al Zubaidi2, Ali Ibrahim Ali Al-Ezzy3*
...aqueous versus alcoholic extracts of Melia azedarach in amelioration of lipid profile, liver enzymes and innate Inflammatory indices for white New Zealand rabbits. Thirty adult white New Zealand male rabbits were classified equally in to 3 groups, G1 were administrated 28mg/day of aqueous extract of Melia azedarach for 14 days, G2, were administrated 28mg/day of ethanolic extract of Melia azedarach for 14 day, G3 administrated pellets and green food for 14 day...
Syed Shahinshah Gilani, S. M. Chaghtai and Umbrin Khan
...experiments. The aqueous extracts from different dried plant parts, soaked for 48 hours, inhibited radicle growth, plumule growth and seed germination of Pennisetum glaucum cv Bari-Hairy. However, no serious inhibition of seed germination was occurred. The toxicity varied from part to part and was related to concentration and soaking duration Root exudates were highly toxic to the radicle growth followed by leaves, stem and whole plant material For the ...
Mohammad Zubair Sulemani* Mohammad Safdar Baloch** and Khalid Abdullah**
...af and litter leachates (extracts) of kikar (Acacia nilotica) on the germination of wheat (Triticum aestivum) maize (Zea mays), sorghum (Sorghum bicolor), millet (Pennisetum typhodeum), sunflower (Helianthus annus), brassica (Brassica compestris) and taramira (Eruca sativa). Seed germination of all sevel crops was significantly and negatively affected due to the application of ext...
G.A. Bajwa and H. Gul
...ortality was heavy....
M. Ismail Chaudhry and Ghulam Ali Bajwa
... the present study water extracts of different parts
of four drug plants Azadirachata indica, Dhatoora alba,
Melia azedarach and Calotropis procera were used to
determine their efficacy against Plecoptera reflexa
larvae, an important defoliator of shisham plantations.
In case of A. indica and M. azedarach seed powder was
used for making 10% water extract and for D. alba and C.
procera leaves, branches and...
Safia Khanum, Farrukh Hussain and Himayat Hussain Naqvi
...f Chloris. Aqueous shoot extracts inhibited radicle growth of Chloris gayana, Panicum antidotale, Pennisetum americanum and Chtysopogon aucheri. Both the species exhibited self-toxicity. The toxicity of each species depended up to the amount of material soaked, soaking duration, freshness of the material assayed and the test species used....
Nasim Akhtar, Himayat Husssain Naqvi and Farrukh Hussain
...xic root exudates, water extracts continued substances which proved to be inhibitory not only to its own growth but also to other species used in the bioassays. The toxicity in both the cases increased with increasing concentration and soaking time. Cinhrus had more allopathic potentiality than Chrysopogon. The toxicity in both the cases was species slated. The possible effect of allelopathy of these grasses is discussed in relation to the vegetation dynamics....
Ammar Abdulhasan Aldhalemi1, Murtadha Abdulhasan Aldhalemi2, Ali Joodi3, Qais R. Lahhob4*
Ammar Abdulhasan Aldhalemi1, Murtadha Abdulhasan Aldhalemi2, Ali Joodi3, Qais R. Lahhob4*
Javairia Mehboob1, Syeda Hafsa Ali1*, Fahima Ashraf Kasi1, Syeda Ayesha Ali2, Safa
Farooqi3, Muneeza Arbab4,
...
three plant extracts and utilizing it in biosynthesis of silver nanoparticles. Green Silver
nanoparticles (AgNPs) were synthesized from 1mM Silver Nitrate (AgNO3) solution incubated
with leaf extracts of Cynara scolymus (Artichoke), Alkanna tinctoria (Alkanet), and
Lavandula angustifolia (Lavender), respectively. The synthesized nanoparticles were
characterized vis...
Muhammad Naeem Korejo1*, Muhammad Nawaz Kandhro1, Aijaz Ahmed Soomro1 and Niaz
Ahmed Wahocho2
...ss (Cynodon dactylon L.) extracts under various
irrigation levels on weed density and yield of mungbean cultivar AEM-96. The experiment
consisted of different weed control practices i.e. weedy check, various levels of sunflower /
bermuda grass extracts, herbicides and hand weeding under three irrigation frequencies (2,
3 and 4). The analysis variance showed that various weed co...
Rizwan Maqbool1, Bilal Ahmad Khan*2, Sumbal Parvez3, Muhammad Ather Nadeem2, Azhar
Hassan1, Jamshaid Qamar2, Ali Nawaz1, Muhammad Adnan2, Rafia Khalid3, Muhammad
Usman2
... The aqueous
extracts of A. officinalis L. were used on radish at different concentrations (2.5%, 5%, 10%,
20%, 40% and 80%). Data regarding seed germination and seedling growth (shoot length,
root length, shoot fresh weight, root dry weigh) of radish were recorded using standard
procedures. Results revealed that the aqueous extract of A. officinalis at higher
concentration (80%) act as bioherbicide a...
Rizwan Maqbool1, Bilal Ahmad Khan*2, Sumbal Parvez3, Muhammad Ather Nadeem2,
Muhammad Mughees Ud Din4, Jamshaid Qamar2, Muhammad Waqas1, Muhammad Mohsin
Amin2, Burhan Khalid1
...eplications. The aqueous extracts of C.
verum. were used on radish and wild pea seeds at different concentrations (2.5%, 5%,
10%, 20%, 40% and 80%). Data regarding seed germination and seedling growth (shoot
length, root length, shoot fresh weight, root dry weigh) of weed were recorded following
standard procedures. Results Reveals that the aqueous extract of A. officinalis at higher
concentration (80...
Hina Gul1, Amjad Usman1*, Karishma1 and Seema Zubair2
...al evaluation of aqueous extracts of indigenous plant extracts in
comparison with synthetic insecticide against Thrips (Scirotothrips dorsalis) on tomato was
conducted under natural field condition during spring 2019. Experiment consisted of 8
treatments (neem extract, tobacco extract, garlic extract, datura extract, lantana extract,
eucalyptus extract, flonicamid (synthetic in...
Hina Ilyas1, Umar Hanif2, Anser Ali1, Zahid Hassan Tarar3,Hamza Javed4, Tehreem Tahir5, Muhammad Rafiq6*
...study, methanolic (MeOH) extracts from leaves, fruit peel and pulp of Citrus bergamia (C. bergamia) and, leaves and fruit of Ficus carica (F. carica) were prepared which were further processed for fractional distillation using ethyl acetate (EA), n-hexane (n-Hx) and chloroform (CHCl3) preparing total 20 extracts aiming to test their anti-tyrosinase potential, in-vitro. Our results confirmed that all C. bergamia and F. carica...
Rizwan Maqbool1, Bilal Ahmad Khan2*, Muhammad Ather Nadeem2, Sumbal Parvez3, Muhammad Mohsin Amin2, Jamshahid Qamar2, Azhar Hassan1, Muhammd Alam Elahi1, Jamshed Haider1, Muhammad Irfan4 and Memuna Ghafoor Shahid5
...eplications. The aqueous extracts of C. verum was used on radish seeds at different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%). Data regarding seed germination and seedling growth (shoot length, root length, shoot fresh weight, root dry weigh) of radish were recorded following standard procedures. Results of our study revealed that the higher concentration acted as bioherbicide and exhibited inhibitory effects on R. sativus while the low concentration it...
Tariq Ahmed Keerio1*, Muhammad Nawaz Kandhro1, Ahmed Naqi Shah1, Muhammad Ibrahim Keerio2, Ghulam Murtaza Jamro3
...mmature and mature plant extracts under various seed rates on weeds density and yield
of m aize cultivar Dadu Maize I The experiment includes different we ed control practices
i.e., control (No weeding) weeding), various levels of sorghum immature and mature plant extracts
herbicides and hand weeding under various seed rates (20, 25, 30 and 35 kg per ha )).
The results showed t...
Siti Fatonah*, Herman and Dewi Yuni Safitri
...ne the effect of aqueous extracts from fresh leaves of C. citratus, M. bracteata and a mixture of both on the germination and growth of Paspalum conjugatum and Borreria alata weeds.Treatments consisted of extracts of M. bracteata, C. citratus and a mixture of both extracts at various concentrations (0% or control, 20% and 60%). Spraying of the extracts s...
Hafiz Muhammad Usama Shaheen1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Ghalib Ayaz Kachelo1, Hadeed Ahmad1, Muhammad Wahab1, Muhammad Faran Tahir1 and Abuzar Hasnain2
... commonly available phytoextracts were explored as potentially safer alternatives to harmful synthetic chemicals for the management of brown leaf spot of rice. For the management of BLS of rice, fourty-four phyto-extracts were screened for their potency in preventing B. oryzae under in-vitro, from where only 8 most effective phyto-extracts were further investigated at different concentrati...
Rauf Khan1, Shazia Mansuri1,2*, Muhammad Abid1, Muneeba Khan2, Anjum Perveen2, Afshan Rehman3 and Rubina Noureen1
...led that all the solvent extracts contained anti-nematodal properties; however, variations in mortality rates were observed. Specifically, the chloroform extract of the leaves of P. boissieri exhibited 84% inhibitory activity against nematodes after 72 hours of exposure. The results indicated that exposure of nematodes for 72 hours resulted in the maximum nematicidal activity compared to exposures of 24 and 48 hours. It was concluded that leaf and stem
Tahani Ahmad Al-Matrafi1, Zuhair M. Mohammedsaleh2, Mamdoh S. Moawadh2, Waheeb S. Aggad3, Rawabi Mohamed almuhimed4, Zamzam Alhuwaymil5, Aishah E Albalawi6, Ifat Alsharif7, Hailah M. Almohaimeed8, Fatima S. Alaryani9 and Mona H. Soliman10,11*
...ethanolic, and ethanolic extracts of Tamarix dioica leaves against paracetamol-induced toxicity. In this study, 36 albino mice were randomly grouped into six groups, each consisting of six mice: Group I (normal control), Group II (paracetamol-toxified), Group III (positive control with Silymarin @ 200 mg/Kg), Group IV (aqueous T. dioica extract @ 400 mg/Kg), Group V (methanolic T. dioica extract @ 400 mg/Kg), and Group VI (ethanolic T. dioica extract @ 400 mg/...
Noor Rahmawati*, Dea Indriani Astuti and Pingkan Aditiwati
.... Endophytic fungi crude extracts have been shown to be able to inhibit DPPH radicals by up to 86 percent by A26 and A19 and by 80 percent by B3 in spectrophotometric studies on cultures with antioxidant activity. This is the first report on endophytic fungus isolates from branches and leaves of T. sinensis that had antioxidant potential. The fungi were identified as Giberrella sp. and Fusarium sp. Future research on these microbes is highly encouraged because...
Suhair Sh. Al-Siraj1, Jihan M. Badr2 and Dalia M.A. El-Masry3*
... (laurusnobilis) aqueous extracts and nano-emulsion against gram-positive (Staphylococcus aureus) and gram-negative (E. coli and Pseudomonas aeruginosa) bacteria and their effects on virulence and antibiotic resistance genes. The MIC) minimum inhibitory concentration (of both was determined using thiazolyl blue tetrazolium bromide (MTT) micro dilution to assess antibacterial activity. Bay leaf (laurusnobilis) extracts and na...
Barkat Ali Jatoi1*, Amjad Hussain Mirani1, Abdul Latif Bhutto1, Ambreen Laghari2, Abdul Samad Magsi3, Ahmed Sultan Jatoi4, Aneela Sultan Jatoi5, Muhammad Mohsen Rahimoon6, Aarab Khan Lund7, Om Parkash1, Atif Ali Malak8
...tus camaldulensis) plant extracts against Pasteurella multocida. A total of 10 farms of each taluka of Shaheed Benazirabad district (i.e. Nawabshah, Qazi Ahmed, Daur and Sakrand) were visited to examine animals suspected of being infected with HS. A total of 2244 buffaloes and 576 buffalo calves were investigated. Among the suspected animals 100 (blood samples) were cultured, and P. multocida isolates were biochemically characterized. The overall prevalence ra...
Waqas Ahmad Shams1*, Gauhar Rehman2, Muhammad Ismail3, Rahmul Kabir4 and Abdul Qahar1
...antioxidant potential of extracts obtained from Crab Himalayapotamon emphysetum using various in vitro assays. Different solvents of varying polarities including ethanol, methanol, ethyl acetate, and water were employed for extraction. The results demonstrated significant antioxidant activity across different extracts in different in vitro assays. Particularly, the aqueous extract exhibited potent 1,1-diphenyl-2-picryl-hydra...
Mukondwa Olivia1, Rugare Joyful Tatenda1, Mabasa Stanford1 and Mandumbu Ronald2*
...nst several weeds. Water extracts of the stems root and leaves of twelve sorghum and pearl millet cultivars were screened for their ability to suppress germination and dry weight of blackjack (Bidens pilosa L.), upright starbur (Acanthospermum hispidium L.) and goose grass [Eleusine indica (L.) Gaertn)] and by the addition of their plant material to soil under laboratory and glasshouse conditions. A randomized complete block design with three replicates was us...
Umm-e-Kulsoom1, Saima Hashim2, Fazli Wahid3, Haseena Gulzar4 and Tamana Bakht5
...et two different aqueous extracts. The objectives of the studies were to investigate the allelopathic effect of extracts of different parts of donor species on weed seed germination and growth of potato. Weed seed germination test was conducted in petri-dishes while potato related studies were conducted in pots. Our data revealed that all the aqueous extracts of all the three donor plants ...
Iqtidar Hussain1*, Mohammad Safdar Baloch, Ejaz Ahmad Khan and Asghar Ali Khan
...ponse of different plant extracts on the morpho-physiological traits of maize crop. The experiment was carried out in a completely randomized design having three repetitions. Water extracts of ten different plants viz., neem (Azadirachta indica L.), eucalyptus (Eucalyptus camaldulensis L.), tobacco (Nicotiana tobacum L.), Indian lilac (Melia azadirach L.), acacia (Acacia nilotica L.), poplar (Populous deltoids L.), sunflower...
Rafaqat Hussain Shah1, Mohammad Safdar Baloch1*, Muhammad Zubair2, Khalid Naveed3, Muhammad Amjad Nadim1 and Aminullah Khan4
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
...t-align: justify;">Plant extracts have been used as alternative additives for the semen preservation process in different farm animal species to improve semen properties such as viability, motility, and membrane integrity, which thereby increase sperm fertility. The current study was conducted to investigate the effect of adding an aqueous extract of Aloe vera leaf gel (AEAV) to the epididymal spermatozoa of local goats during the cooling process for different...
Afnan Ahmed Allhyani1, Mohamed Baeshen1, Nagwa Thabet Elsharawy2,3*
...examine Moringa oleifera extracts as natural preservatives by examining its antibacterial effect by (aquas & ethanolic) extracts by different concentrations (1.25, 2.5, 5, 7.5, 10, 12.5 & 15) cc against six foodborne microorganisms (Listeria monocytogenes, Staphylococcus aureus, Salmonella typhi, Escherichia coli, salmonella enteritidis and Vibrio parahaemolyticus). Finally, the different concretions of each extract ...
Abdel Fattah A. Khalaf1, Karam T. Hussein1, Shehta A. Ali2, Doaa K. Barakat2*, Mohamed I. Gad1
...Fixed oils and ethanolic extracts affected the levels of total protein, fat, and total body carbohydrate. The obtained results concluded that the two fixed oils (Croton tiglum and Simmondsia chinensis) and the two ethanolic extracts (Urtica dioica and Hyoscyamus muticus) can be used as safe alternatives to the chemical insecticides.
Keywords: Plant extracts...
Saima Maher1*, Salma javed2*, Gul mina1, Rizwana Salam1, Farah Mukhttar1, Noureen Khan3 and Fakhra Batool3
...rom which five different extracts methanol, ethanol, ethyl acetate, hexane, and chloroform were prepared from various plant. Preliminary phytochemical analysis of these extracts revealed the existence of main phytochemical constitutes, including alkaloids, glycosides, flavonoids, tannins and phenolic compounds. These compounds were found to contribute to the observed anti-nematicidal activity of the plant
Elham M. Hosny1*, Saad Mahmoud Saad1, Marionette Zaghloul Nassif2, Amani Mohamed Salem1
...s and propolis ethanolic extracts work as natural antifungal agents against Candida albicans, both in vivo and in vitro. The broth microdilution assay was used for testing how well Spirulina platensis and propolis extracts inhibit fungal growth. The minimum inhibitory concentrations (MIC) of Spirulina platensis (SPP) and propolis extracts (PEE) against Candida albicans were determined to b...
Hina Yasin1,2*, Shaukat Mahmud2, Hina Abrar1, Kaneez Fatima3, Rabia Bushra1 and Saima Zahid2
...nistering various leaves extracts in differential dose pattern (81, 54 and 27 mg/kg). Cytotoxic activity was assessed by the Brine shrimp bioassay method. Results showed that extracts significantly responded as analgesic drug comparable to standard drug using different methods of evaluation for analgesic activity. Brine shrimp bioassay in different leaves extracts also showed similar effec...
Kouakou Yadom Yao François-Regis*, Kra Kouamé Daniel and Atta Diallo Hortense
...-knot nematodes. Aqueous extracts of five types of castor leaves were prepared. These were immature (ILV) and mature (MLV) leaves from vegetative plants and immature (ILF) and mature (MLF) leaves from flowering plants. The fifth type (ATL), a mixture of all leaf types, was used as a control. Second-stage juveniles and eggs of M. incognita were exposed to extracts for 72 hours and 10 days, respectively. Egg-hatching inhibitio...
Estrelle Anne Tacbas1, Neil Pep Dave Sumaya2 and Nanette Hope Sumaya1,3*
...nins in both plant crude extracts. Different developmental stages of C. elegans (i.e., 1st to 4th larval stages (L1-L4), young adult (YA) and adult nematodes) were used for in vitro anthelmintic assay, mortality, development of life stages, and reproduction of the nematode. Both plant extracts caused high mortality in the L4 stage for their LC50 and LC90 values. Notably, the crude extracts...
Muhmmad Bilal Islam1* and Sar Zamin Khan2
Emad M. Gad1, Haidy G. Abdel-Rahman2, Mohy Eldin Abd-El-Fattah1, Merna M. Kamal1, Ahmed Shaker Eltahan3, Amina A. Dessouki3*
...ulberry fruit and leaves extracts and their combination on the kidney of diabetic rats. To induce diabetic nephropathy, experimental rats were supplied with 10% fructose (Fr) in drinking water for the first two weeks. Each Fr-fed animal received an intraperitoneal injection of a low single dose of STZ (40 mg/kg) after being fasted for the whole night. Sixty albino rats were separated into six equivalent groups. Group I control rats administrated 0.5ml distille...
Sana Eijaz, Asmat Salim* and Mohammad Anwar Waqar
...cin and treated with the extracts. Glucose level and body weight was checked throughout the study. The expression of insulin signaling genes was observed in skeletal muscle and adipose tissue by performing RT-PCR. Moreover, the level of adipokines was measured by using ELISA. Data was analyzed by t-test and One-way ANOVA using SPSS. Significant improvement was observed in the blood glucose level and body weight of diabetic rats that received the herbal combina...
Nur Anis Hashim1, Nor Hasima Mahmod1*, Abubakar Abdullahi Lema2, Lee-Hoon Ho3 and Mohammad Moneruzzaman Khandaker1
...roselle calyx, and these extracts were used to determine antioxidant activities and contents by assessing DPPH scavenging activity, total phenolic content (TPC) and total flavonoid content (TFC). Next, the samples were subjected to qualitative phytochemical screenings. DPPH scavenging activity was the highest in an FC sample extracted by methanol with 81.697 mg/mL. The highest TPC was measured in an OD sample extracted by ethanol (172.04 ±0.43) and the ...
Aamir Khan Awan1, Nighat Sultana1*, Rahmat Ali Khan2, Rifhat Sultana3,
Umm-e-Kalsoom1, Fayaz Ahmed Sahibzada4, Rifat Ullah Khan5, Naimat Ullah Khan6, Nazir Ahmad Khan7, Syed Haider Zaman8, Mir Sadiq Shah9 and Assar Ali Shah10*
...als of P. granatum peels extracts in alloxan-induced diabetic albino rats. The diabetes was induced in albino rats using alloxan at the concentration of 150 mg kg-1 body weight. After 15 days, the treated groups with glabinclemide and different varieties of methanolic and aqueous extracts showed a significant (P<0.05) decrease in the blood glucose level as compared to diabetic control while slight differences were seen in...
Muhammad Salim1,2*, Muhammad Zakria2, Ahmad Ur Rahman Saljoqi2, Shahid Sattar2, Ayhan Gökçe1, Amjad Usman3 and Hayat Badshah4
...thenium hysterophorus L. extracts against wheat aphid, Sitobion avenae (Hemiptera: Aphididae) and lady bird beetle under field conditions was conducted at Agricultural Research Institute (ARI) Tarnab, Peshawar, Pakistan from December 2019 to June 2020. The experiment was consisted of five aqueous extracts of different parts of P. hysterophorus (whole plant, stem, leaves and flower extracts...
Asfand Yar Khan1, Syed Saleem Ahmad1*, Muhammad Avais1 and Kamran Ashraf2
...ifferent medicinal plant extracts against Shiga toxin (E. coli O157:H7) based on the in-vitro and in-vivo trial. A total of twenty-one (n=21) PCR-confirmed isolates were selected for detection of virulent genes in-vitro trial (zone of inhibition). For in-vivo trial, forty-two (n=42) day-old chicks were divided into seven equal groups randomly (n=6 birds/group), and were classified as: G1 (Azadirachta indica @ 18.25 mg/ml), G2 (Melia azedarach @ 15.75 mg/ml), G...
Mohamed E. Enany1, Ahmed M. Hamouda2, Reem M. Khashaba3*
...ation with natural plant extracts. Turkey poults were assayed for the presence of Enterobacteriaceae species with prevalence 40% (40/100) of the tested samples. Escherichia coli has the highest prevalence rate with 77.5% followed by klebsiella spp and Salmonella spp. with7.5%and 5% respectively.Resistance of the collected Enterobacteriaceae strains was determined by the Kirby-Bauer disk diffusion test against a panel of antibiotics.. The isolates were highly r...
Rand Kamil Abbas, Zahra Sadoon Hadi*, Ahmad Hassan Sahib
... mg/kg of aqueous garlic extracts for seven days). The impact of parasites on various blood parameters were investigated and died animals were examined for gross and histological changes. The results revealed that trichomonas had affected the blood picture, decreased Hb and PCV concentrations while dramatically increasing WBC with no discernible effect on RBCs. On the other hand, garlic possessed beneficial effects on blood parameters and bird health. Gross cl...
Oluwatoyin Adenike Fabiyi* and Abigail Abosede Olojede
...age plants using aqueous extracts from Bixa orellena as reductant. Analysis of variance (ANOVA), statistical method was explored using GenStat 5.32 to achieve this. Formation of nanoparticles (NPs) was confirmed by reaction solution colour change, Fourier Transform Infra-red and Scanning Electron Microscopy (FTIR and SEM). Strong peaks on the FTIR spectrum were observed at 3356, 3360 and 1637 cm-1, indicating that amines are the reducing and stabilising factor...
Saima Bibi* and Nazir Ahmad
...luated the impact of bio-extracts (Phenolic compounds) and commercial tannic acid on the nutrient composition and in sacco digestibility of berseem fodder in three non-lactating ruminally cannulated Holstein cows at the University of Agriculture Peshawar, Pakistan. The cows were fed a diet of 90% fodder and 10% concentrate on a dry matter basis. Five Plant-derived phenolic compounds and commercial tannic acid at concentration of 1.5%, 3.0% and 4.5% were used. ...
Beenish Khanzada1*, Shagufta Jabeen2, Asif Ali1, Amna Fatimah1, Ishfa Zahid1, Esha Israr1, Arifa Iqbal1 and Ashas Waheed1
...s. The traditional plant extracts known for their antimicrobial or antioxidant properties can be incorporated in biopolymer based film forming solution to make active packaging films. This study contributes to comparative analysis of synthesis of biodegradable films from two biopolymers (i.e., starch and chitosan) with the additive effect of two medicinal plant extracts i.e., Pithecellobium dulce (PD) and Cassia senna alata ...
Heba Saleh Shaheed1, Zainab J. Malik2*, Ghusoon A.A. Alneamah1, Salih T.H.2
Abdulameer Jawad Zayier*
...prepare several types of extracts (aqueous, hot, cold, alcoholic, and nano) from the colocynth plant to determine the inhibitory effect of these extracts on the number and vitality of the Leishmania tropica promastigotes in vitro ,The results shown that even at low concentrations (0.5 mg), the colocynth plant’s green-produced silver nanoparticles demonstrated strong antileishmanial activity against Leishmania tro...
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