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Matheus Nunes Weber1, Simone Silveira1, André Felipe Streck1, Luís Gustavo Corbellini2, Cláudio Wageck Canal1*

... the near future.

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Bibi Tayyaba Batool1, Aemal Tareen1, Shahjahan Shabir Ahmed1, Hira Ejaz1, Mohammad Azam Kakar1, Syed Abdul Rehman2, Mohammed Saeed1, Zafar Ahmad3, Mohammad Masood Tariq3, Mohammad Arif Awan3 and Muhammad Naeem Shahwani1*

...stify;">Brucellosis and bovine tuberculosis (TB) are a constraint to livestock production in Balochistan. The aim of this study was to determine the prevalence of brucellosis and bovine tuberculosis in cattle, goats and sheep in some primary dwelling districts of Balochistan. Our results showed that brucellosis was found 2.2% positive from Quetta district and 0.6% positive from Pishin district and bo...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...al patellar fixation in bovine.
...
Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2
... commercially available bovine F. hepatica enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this test indicated significant (p<0.05) values for she...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...ucleotide sequencing of bovine osteopontin gene showed a novel DNA polymorphism 37421762 (C>A) in the 3’-untranslated region (3’-UTR) of the gene. Association test was performed to analyze its relation with milk composition but it was found non-significant in all analyzed traits. So, it was inferred that it might be due to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identi...

Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3

...were positive (n=6) when ovine sera were tested. The study revealed that PPR-FMD viruses were circulating in the region and warrants proper control measures. 
...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
... levels were studied in bovine from three distinct temporal zones of Khyber Pakhtunkhwa province of Pakistan. Twelve hundreds ticks were collected from four hundreds animals comprising of two hundred and fifty cattle and one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamaph...
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5 
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
... mammals such as human, bovine, pig and rabbit, it also has major influence in the natural defense of the animal body. In this paper, by comparative study on BPI gene expression in various tissues between Rongchang pig and Landrace, we confirmed the expression of pig BPI gene in various tissues to determine the difference expression of antibacterial related gene among indigenous breed (Rongchang pig) and artificially cultivated breed (Landrace), The Rongchang ...

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

...s used for diagnosis of bovine brucellosis on serological, bacteriological and molecular basis. A total of 141 cows from brucella infected farms under quarantine of the veterinary authorities were employed. Serological examination using BPAT, RBT and CFT in 141 cows revealed 109 (77. 3), 105 (74.47) and 104 (73.76) seropositive respectively. Relative sensitivity, relative specificity, positive predictive value and negative predictive value of BPAT, RBT and CFT...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...an overall prevalence of ovine theileriosis as 27.5%, 13% and 19.5% in districts of Dera Ismail Khan, Tank and Bannu, respectively. Sex, tick infestation, animal keeping conditions, herd size, season and feeding pattern were observed as significant factors associated with the disease occurrence. On the other hand, the effect of age and breed on the occurrence of disease was observed as non-significant. Biochemical examination revealed a significant increase in...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
... coli isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli<...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
... the prevalence of BVDV(Bovine Viral Diarrhea Virus) in yaks in Hongyuan, Sichuan province, a cold climate and high altitude area of China. Totally, 516 yak serum samples were randomly got during 2012 to 2014. BVDV was found highly prevalent among the adult yaks (51.1%; 95% CI: 46.5-55.6) and calves (60.5%; 95% CI: 43.4-76.0), respectively. The prevalence of BVDV infection in 2012 to 2014 was 45.3% (95% CI: 38.6%-52.1%); 56.0% (95% CI: 49.7-62.2) and 60.5% (95...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...on in the four domestic bovine species of Pakistan, which are normally less aggressive. Selected animal species were cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V158M is present in exon-8 of COMT gene. Each specie was subjected to blood sampling (n=20 each). DNA was extracted, purified, quantified and amplified through PCR by using specific primer set. Then Sanger&rsqu...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...trength and mobility of bovine serum albumin (BSA) was different in all milk species.
...
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4,    Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...ociated risk factors of bovine tuberculosis (bTB) among large ruminants of the five districts (Peshawar, Nowshera, Charsadda, Mardan and Swabi) located in the central zone of Khyber Pakhtunkhwa (KPK), Pakistan. A total of 2400 large ruminants were sampled by convenience sampling approach and tested by comparative cervical intradermal tuberculin test (CCIT) for detection of Mycobacterium bovis infection. Data regarding socio-demographic status, risk fact...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2

 

.... Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more prone to disease. In the case of buffalo breeds, prevalence was more in Nili Ravi buffalo, i.e. 36.6% than Kundi breed, i.e. 33.3%. Prevalence of disease was found maximum in humid and plain area of Lahore, i.e. 56%. In dry, dusty and hot weather of ...

Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1

Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...trade;) for controlling bovine mastitis through systemic dry period therapy,
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation and at 14th day pre calving while group G2
was kept as control. Samples of mi...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...poradically reported in bovines in Pakistan. During June 2014 to 2015, twenty five buffalos ranging in age between 7 to 9 years, were referred to surgery clinic at University of Veterinary and Animal Sciences, Lahore, Pakistan. The patients showed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure an...

Omer Baris Ince* 

...cally healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the disease-related status of the selected region, to investigate the active virus circulation and determine the antibodies formed against the nonstructural proteins (NSP)...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...iola sp. infestated bovines (cattle and buffaloes) and their effect on various blood parameters (WBC, RBC, Hb, HCT and MCHC). For this purpose, 40 infected animals were selected. After dividing them randomly in four groups of 10 animals each, drug was administered to first three groups whereas last one was kept under positive control for comparison. Study revealed that Triclabendazole was most effective drug of all three in both cattle and buffaloes with e...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

... the causative agent of bovine mastitis, Staphylococcus aureus. Ninety milk samples were collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (SFMT) was applied for screening subclinical mastitis f...
Muhammad Zain Saleem1,*, Raheela Akhtar1, Asim Aslam1, Muhammad Imran Rashid2, Zafar Iqbal Chaudhry1, Muhammad Adeel Manzoor1, Bilal Ahmed Shah3, Rais Ahmed4 and Muhammad Yasin5
.../i> (causative agent of bovine) may cross the species barrier to infect the small ruminants which may complicate the control measures of brucellosis because most of the control programs rely on serological screening of brucellosis rather than molecular assay which could confirm the particular species circulating in ruminants. In this study 960 and 471 serum samples of goats and sheep were collected, respectively. After screening with Rose Bengal test (RBT), al...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

...dairy animals including bovines. However, only a little information is available about bacterial profile of the disease. A cross-sectional study was conducted to find the Etio-prevalence of bacterial species causing subclinical mastitis in a cohort of buffaloes at Khyber Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was p...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
 

Aftab Shaukat1,*, Khalid Medmood3, Irfan Shaukat2, Tauseef ur Rehman3, Muhammad Ahsan Naeem1,5, Ashar Mehfooz1, Muhammad Ijaz Saleem1, Zia-ud-Din Sindhu1, Shahid Ali Rajput4, Mubashar Hassan1,4, Saqib Umar1, Muhammad Ali Jamil1, Rao Zahid Abbas1 and Anas Sarwar Qureshi1

 

...logical changes (due to bovine anaplasmosis) were studied in Sahiwal cattle and crossbred cattle of the district Faisalabad of Punjab province, Pakistan. Therapeutic efficiency of Oxytetracycline-Imidocarb dipropionate combination was compared with Enrofloxacin-Imidocarb dipropionate against Anaplasma marginale (A. marginale). For this study, the blood samples of three hundred and sixty nine cattle were collected. Giemsa stain of blood smears sho...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...fect of polymorphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinical (n=50) mastitis along with non-mastitic (n=50) cows from different dairy farms of Punjab. TNF-α gene was amplified with specific primer and sequenced to get the full-length sequen...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...cal isolates.
...
Ambreen Leghari1,2, Asghar Ali Kamboh2,*, Shakeel Ahmed Lakho1, Faiz Muhammad Khand1, Kanwar Kumar Malhi2,3, Iqra Bano Chandio4, Seema Baloch5 and Jan Mohammad Shah4
...on on the prevalence of bovine tuberculosis (BTB) in randomly selected cattle (n= 160) was carried out in Hyderabad and Tando Allahyar districts. The animals were first screened through single intradermal tuberculin test (SITT) and both positive and negative reactors of SITT were further investigated through rapid test. Attempts were also made to isolate the Mycobacterium bovis organism from the milk and nasal secretions of cattle using the Lowenstein-J...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...ndogenous gene ABCA1 in bovine mammary epithelial cells (BMECs) to analyse the effect on intracellular triglyceride (TG) content by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, cell TG Assay and RT2 Profiler PCR array. The results showed that ABCA1 knockdown reduced the TG content in BMECs. On the other hand, the RT2 Profiler PCR array demonstrated that the expression levels of 12 genes, including fatty acid...
Memis Ozdemir
...n 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in Bos taurus cattle breeds, but have been not detected the RsaI restriction enzyme digestion site in <...

Ankur Abrol, Madhumeet Singh, Akshay Sharma* and Pravesh Kumar 

Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2,  Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1Aneela Zameer Durrani4 and Muhammad Usman4*
...e.
...

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

...igate the prevalence of bovine tuberculosis (bTB); detection of Mycobacterium bovis (M. bovis) infection in lactating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalen...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...uality of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buse...

Asmaa Abdallah Darwish 

...participate in different ovine diseases pathogenesis and complications and their supplement for healthy and diseased animals is strongly recommended.  

...

Md. Zubael Islam, Rukhsana Amin Runa and Md. Mahmudul Alam* 

...tential risk factors of bovine foot diseases in certain areas of Sirajgang district. It is recommended that further robust studies are required to address the foot disease burden in lactating cows. 

...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...notic Moroccan goat and bovine strains. The amino acid sequences of the antigenic regions inside VP8* proteins showed high conservation between the amino acid sequences of human and animal strains bearing P[14] proteins, and revealed several changes with respect to the RotaTeq™ and Rotarix™ vaccine strains. This report suggests a possible reassortment event between human and domestic animal rotaviruses. Continuous surveillance in the post vaccine e...

Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1 

AGE EFFECT ON THE MECHANICAL PROPERTIES OF HIP JOINT BONE: AN EXPERIMENTAL INVESTIGATION
... bone specimens of male bovine of different age groups were tested for fracture toughness, young modulus
and tensile strength in the longitudinal and transverse directions of the collagen’s fibers. Compact tension specimen
was used for the fracture toughness, while for young modulus and tensile strength, rectangular flat specimens were
used. The test procedures were similar to ASTM (American standard for testing and measurements) tec...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
.... In the present study, bovine skeletal muscle satellite cells (bSMSCs) were cultured and the Wnt/β-catenin signaling pathway was activated by LiCl. Investigation of the patterns of differentially-expressed miRNAs and analysis of bSMSCs target genes were then carried out using microarray and bioinformatics. The data revealed significant changes (>2-fold) in 29 out of 783 differentially expressed miRNAs, 12 of which were up-regulated and 17 were down-re...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
...i> muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated ce...

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
...uding bluetongue, sheep, ovine, goat, caprine, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After reviewing 82 published articles, a total of 48 studies from 29 articles were eligible to be included in this meta-analysis study. The total seroprevalence of bluetongue in apparently healthy sheep, goat, cow and camel at animal level based on ELISA test was ...
Muhammad Riaz1*, Zahida Tasawar1, Muhammad Zaka Ullah2 and Zawar Hussain3
Parasitological and Molecular Survey on Theileriosis of Sheep and Goats and the Related Risk Factors in Musa Pak Shaheed Town, Multan, Pakistan
...plification to determine ovine and caprine theileriosis. Through a questionnaire, the data regarding animals as well as herds characteristics were gathered to define the risk factors favors the spread of Theileria spp. infection. 24% and 8.5% Theileria infection identified through PCR and microscopic examination during present investigation. The PCR detected 14.5% and 9.5% Theileria piroplasms infection in sheep and goats, respectively. Among Theileria species...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...iability of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult bovine serum (ABS) to be evaluated as alternative to FBS. ATFs were cultured in DMEM-HG and supplemented with 10% FBS, 10% AES and 10% ABS. At passage 4, each group showed same morphology and appearance, there wa...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...yle="font-size: small;">Bovine luteinizing hormone receptor (bLHR) gene in mammals is important for several reproduction function such as steroidogenesis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasunda...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
...yle="font-size: small;">Bovine leukemia virus (BLV) is the cause of enzootic bovine leukosis and has worldwide distribution. This is study reports presence and genetic diversity of BLV in cattle of two districts of Punjab, Pakistan. Blood samples (n=400) taken from local (n=200) and exotic (n=200) breeds of cattle were processed for complete blood count. Based on lymphocyte counts, samples were divided into two groups (A, ly...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...n of betacoronavirus 1 (bovine coronavirus) BCoV in newborn gastroenteritis calves in the Delta Region, Menofia Province, Egypt, also for isolation and identification of the local circulating BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections which leads to serious complication which may be fatal in young calves up to 3 months o...

Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*

...es on the prevalence of bovine surgical affections in Mymensingh and its surrounding localities.

...

Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*

...y;">Buffaloes and other bovines suffer from a fatal disease, “Hemorrhagic septicemia” (HS), which is produced by a gram-negative bacterium “Pasteurella multocida.” Existing literature has established bacteria’s pathogenesis, but still, the literature about the role of bacteria and its immunogens on the demonstration of clinical signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study ...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...rk, the sequence of the bovine STAT5A gene was analyzed to identify single nucleotide polymorphisms and its effect on evolutionary divergence. Nine polymorphisms, six in intronic and three in exonic regions, were identified in STAT5A gene. One exonic polymorphism G→A, in exon 5, was significant that causes a non-synonymous amino acid change from Serine (S) to Asparagine (N). Further, 64 genetic variants were also identified in STAT5A ...

Muhammad Yousif Jakhrai1, Ahmed Nawaz Tunio2, Ali Mujtaba Shah1*, Tarique Ahmed Khokhar1, Muhammad Mohsen Rahimoon1

...minants like caprine and ovine. The information was obtained from published papers of last twenty years via research gateways such as; PubMed, Scopus and Google Scholar. Small ruminant anesthesia, local anesthesia in ruminants and high epidural analgesia in sheep and goats were key points for this review. However, these research papers were included in this study, were published between from 1999 to 2017. The bibliographies of these references were also used f...
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
...polysaccharide (LPS) in bovine mammary epithelial cell line MAC-T cells. Cells were cultured for 48 h with different LPS levels (0, 0.1, 0.5, 2.5, 12.5, and 100 ng/mL). The results showed that cell viability was negatively correlated with LPS concentrations. The production of reactive oxygen species increased in a dose-depend manner. Cellular concentrations of oxidative damage markers were positively correlated with applied LPS concentrations. Total antioxidan...
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...ots was investigated on bovine herpes-1 (BHV-1) infection in vitro. An ethanolic
extract of Salvadora persica was used in two concentrations. MDBK cells grown on
Minimum Essential Medium (MEM) were used for virus propagation and titration. The
effects of two concentrations of Salvadora persica on viral growth in MDBK cells as
well as cytolytic activity of BHV-1 were evaluated at different times pretreatment
...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...ogous with the vaccinal bovine strain.

...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...passages in Madin Darby Bovine Kidney (MDBK) and identification of isolated virus was done by indirect immunofluorescence test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The results confirmed infection of calves with lumpy skin disease virus (LSDV) and showed that calves older than 3 months old age and those out of first calf heifers are more suscept...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...designed to investigate bovine rotavirus antigen in fecal samples of forty diarrheic new borne calves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...-11 day and Madin Darby bovine kidney (MDBK) cell culture. Identification of virus by indirect immuno fluorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by ...

El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.

...report the isolation of bovine ephemeral fever virus (BEFV) field strains from clinically infected cattle and buffaloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutralization and im...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

...isease diagnosis.

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Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2

... tests for detection of bovine viral diarrhea virus as possible extraneous virus contaminant using RT-PCR and detection of mycoplasma as possible bacterial contaminant using PCR technique. All of the four batches were positive when tested using specific primers and they were free from BVDV or mycoplasma contamination. Results in this study showed that molecular techniques could be used for rapid evaluation of PPR vaccine including RT-PCR for identity testing a...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...yle="font-size: small;">Bovine anaplasmosis is an infectious tick-borne disease caused by a rickettsial parasite Anaplasma marginale. This study investigated the prevalence of A. marginale in cattle on different commercial farms in subtropical conditions of Khyber Pakhtunkhwa (KP). A total of 120 cattle from different farms were randomly sampled and tested for the presence of A. marginale between May to August...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...v>
on Madin-Derby bovine kidney cell line ( MDBK ); confirmation of LSD isolated virus was done by
using Electron microscopy (EM) on suspension of cell culture passage of the virus, Serological
identification was done by serum neutralization test ( SNT ) using reference antisera which confirm
presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR)
gene segment of the...

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

...titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective d...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*

Yonis Abukar Mohamed1, Md. Nazrul Islam1, Md. Haydar Ali1, Mahfuza Akther1 and Abdiaziz Idiris Mohamud2*

Muhammad Zahid1, Aneela Zameer Durrani1, Muhammad Ijaz1, Khushi Muhammad2,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal3

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

...e: smaller;">Keywords | Bovine mastitis, Causal agents, CMT, Prevalence, Risk factors 

...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*

Asad Ullah1*, Umar Sadique2, Ibadullah Jan2, Imad Khan1, Raheela Taj3, Mumtaz Ali Khan4, Salah-ud-din4, Naimat Ullah Khan1

...ium tuberculosis (MTB). Bovine tuberculosis (bTB) is one amongst neglected zoonotic disease (NZD) caused by Mycobacterium bovis; has an economic importance with public health significance and life-threatening infection at the human-animal-ecosystem interface in low-resource settings and developing countries globally. Our study aimed to investigate the incidence of pulmonic tuberculosis caused by M. bovis in specific occupational groups; to study the socio-demo...
Muhammad Qasim Mazari1, Dildar Hussain Kalhoro1,*, Hasina Baloch1, Muhammad Saleem Kalhoro2, Shahid Hussain Abro1, Rehana Buriro1, Asmatullah Kaka1, Fahmida Parveen1, Mazhar Hussain Mangi3, Ghulam Murtaza Lochi1, Abdul Ahad Soomro4, Ali Gul Soomro1, Abdul Ghaffar Abbasi1 and Sarfraz Hussain Depar1
...ectively. Prevalence of bovine tuberculosis in Mirpurkhas district via cultural examination was recorded as 1.66%, 2.5% in milk and nasal samples, while in district Badin it was 1.66%, 5%, respectively. The overall prevalence of bovine tuberculosis was 5.5% and 7% in Mirpurkhas and Badin districts through tuberculin, Rapid BTB Ab, Culture examination and ELISA test. Different tests such as SITT, Rapid BTB Ab, culture examina...

Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra

...nimal productivity. The bovine GH has been studied quite thoroughly but very little information regarding Buffalo growth hormone is available in literature. Buffalo is an important source of milk in Asia and there is a need to identify variations in the genes of buffalo GH and its possible effects in milk production. The present research was carried out to explore DNA polymorphism in buffalo growth hormone gene. In this study we amplified a 5’ flanking r...

Xuelong Chen1, Honghai Wang2, Wenchao Li1, Weina Guo1 and Yanping Qi1*

...="text-align: justify;">Bovine herpesvirus 1 (BHV-1) is a virus capable of causing serious respiratory distress in yaks, and it can further result in reproductive diseases and late-term abortions. In this article, we conducted a systematic meta-analysis aimed at exploring BHV-1 prevalence in yaks of the Chinese Qinghai-Tibetan Plateau (QTP). To complete this analysis, we identified all relevant articles on this topic in both English and Chinese literature data...

Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2  

...use several diseases in bovine animals including mastitis. The prevalence of mycoplasma mastitis is reported from many parts of the world in farm animals. The response of antimicrobials against this infection is very poor that usually lead to culling of infected animals. In Pakistan, unfortunately no data is available on the occurrence of mycoplasma mastitis in farm animals. Therefore, current field investigation was conducted first time in centre-west Balochi...

Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1* 

...e the seropositivity of bovine leptospirosis in dairy animals and associated risk factors at the farm level. The semi-structured questionnaire was designed as per the literature and used to collect leptospirosis associated risk factors along with serum samples from eight non-vaccinated dairy herds. The collected serum samples (n=56) were examined for detection of leptospiral antibodies using the Microscopic Agglutination Test (MAT), the gold standard serologic...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Shaukat Ali1, Muhammad Farooq Bhatti2, Azizullah1, Shafaat Yar Khan3, Abdul Khaliq4

...e Amway protein, honey, bovine milk, sericin, probiotics (Bacillus cereus, B. subtilis, B. amyloliquefaciens, B. licheniformis, Lactobacillus casei, Saccharomyces cerevisiae and Spirulina), vitamins (C and E), royal jelly, ascorbic acid, cowpea seed powder, AgNPs, secondary metabolites (phenols flavonoids, phenolic amino acids and proline) and white hen’s egg at different larval instars. Economic parameters (pupal weight, shell ratio (%), cocoon weight, ...

Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*

...o analyze the effect of bovine pituitary extract (BPE) on increasing the number of offspring per birth and the concentration of steroids (estrogen and progesterone) in the local rabbits. In this study, we used 10 non pregnant rabbits that have given birth and were healthy clinically. All rabbits were randomly divided into two treatment groups, namely control group (K1) and treatment group (K2) where each group consist of five rabbits. The rabbits of K1 group w...

Muhammad Zahid Iqbal1, Aneela Zameer Durrani1, Jawaria Ali Khan1, Nisar Ahmad2, Muhammad Usman1,*, Abdul Jabbar1, Saba Usman3, Ahsan Anjum3, Muhammad Husnain1, Nadeem Raza1 and Anwar-ul-Haq4

...uses query (Q) fever in bovines. Its role has never been elucidated in bovines from Pakistan. The current study was designed to determine the incidence of coxiellosis in bovines, evaluate the association of various risk factors in the occurrence of Coxiella burnetii, determine phylogeny and genetic variability of various isolates identified during the study, and report hematology of the af...

Ouedraogo Oumar1,2, Tianhoun Denté Fidèle2,4*, Séré Modou3, Kaboré Adama2, Tamboura H. Hamidou2 and Belem Adrien Marie Gaston4

...o-economic incidence of bovine tuberculosis. For this purpose, daily monitoring of cattle from various farms and slaughtered at the slaughter area was carried out from February to June 2019 during ante and post-mortem inspections. The results of the analysis of the data collected during these inspections revealed that 1,716 cattle of local breeds, consisting of 875 females and 841 males, were slaughtered and checked by the veterinary inspection officers. At po...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5

Sania Subhan Qureshi*,1, Baitullah Khan1, Shahid Khan1, Hanif Ur Rahman1 and Muhammad Subhan Qureshi2

...ooted animals including bovine, caprine and ovine. Globally, FMD production losses are up to 21 billion US dollars annually. Annual losses in Pakistan is 692 million dollars in terms of body weight loss, milk production, treatment cost and mortality. FMD is caused by 7 different serotypes of FMD virus having different virulence. To compare the virulence of various FMD serotypes the study was designed to propagate the virus o...

Zahra Jamilah Sabrina1, Adrian Pearl Gunawan2, Beryl Reinaldo Chandra2, Ilham Pangestu Harwoko3, Johannes Marulitua Nainggolan4,Widi Nugroho1* 

...-CHA) and nano-CHA from bovine bone combined with Platelet-Rich Plasma (PRP) was hypothesized to accelerate fracture healing. This study aimed to characterise micro-CHA and nano-CHA synthesized from bovine bone and analyze their effects as xenografts on fracture healing in Rattus norvegicus models. The micro-CHA was synthesized using thermal treatment at 720oC, and nano-CHA was made from the micro-CHA using nano-milling. The...

Duong Thanh Hai1*, Tomas J. Acosta2, Tran Thi Minh Tu3

...pha (PGF) secretions by bovine endometrium around the time of ovulation. In preliminary experiment, doses of PF915275 (PF, 11-β hydroxysteroid dehydrogenase type 1 inhibitor) was exanimated. PF at a dose of 1000 nM (PF1000) decreased significantly the absolute conversion of Cs to Cr (p<0.05). Thus, the PF1000 was chosen for the further studies. Tissues of endometrium were collected at the follicular stage (days 19-21), and exposed to Cs (300 nM) or Cs ...
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...ifestations to those of bovine viral diarrhea virus infections. Although, HoBi-like Pestivirus infection has been recorded in multiple countries as in South America, Europe, and Asia, no clinical cases were recorded in Egypt even now. Here, for the first time, we reported natural infection of HoBi-like Pestivirus in Egyptian cattle. Serum and ovarian samples were collected from cattle during the year of 2019. RT-PCR, viral isolation, sequencing, and phylogeny ...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...="text-align: justify;">Bovine alphaherpesvirus 1 (BoHV-1) is an enveloped double-stranded DNA virus of Herpesviridae family. Closely related alpha herpesviruses; BoHV-1 and Bovine herpes virus-5 (BoHV-5) have the same antigenic and genetic relatedness. BoHV-1 is one of the viruses that incriminated in respiratory disease; affects the genital tract and causes significant economic losses to domestic cattle in Egypt. Although ...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

Zohair S. Mulla1*, Mostafa M. Abdelhafeez2 

...logical status of three bovine meat products namely, ground beef, beef burger, and beef sausage retailed in Saudi Arabia. Bacteriological examinations involved the estimation of total bacterial count (TBC), total psychrophilic count (TPsC), most probable number (MPN) of coliforms, and MPN of E. coli. In addition, isolation, and identification of shiga toxin producing E. coli were further conducted. An experimental trial was done for improvement of the bacterio...

Muhammad Kashif Maan1*, Rohma Shabbir2 

Md. Sabuj Rahman and Md. Mahmudul Alam*

... A 2-day-old cross-bred bovine calf was brought to Veterinary Teaching Hospital, Bangladesh Agricultural University, Mymensingh, Bangladesh having atresia coli with atresia ani et recti. Bypass surgery was performed using incisional cecostomy techniques. After 10 days post-surgical administration of antibiotics and non-steroidal anti-inflammatory drugs, a good prognosis in the calf was noticed. After 1 month, a mild skin problem occurred which was minimized si...

Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1 

...cow, Uteri  

...

Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1 

...ductive technologies in bovine. However, Plant extracts has lately appeared as a low-cost, it considers a natural source of chemicals for preserving, also enhancing activity of sperm during different period storage. Current paper gives an apprise on the usage of garlic (Allium sativum) extract as an alternative additive for sperm preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental pr...

Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3

...gies for the control of bovine mastitis.

...

Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov* 

...of cow pregnancy is the bovine pregnancy-associated glycoprotein 1 (boPAG1) antigen. The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1. We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence. Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed. But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expresse...

Amoon Danial1, Shahan Azeem1*, Sohail Raza1 and Muhammad Hassan Mushtaq2

Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*

... in humans, horses, and bovines, but rarely in ducks. We isolated and cultured cartilage stem/progenitor cells (CPSCs) from the articular cartilage of 14-day-old Peking duck embryos using the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clonogenic effic...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...cal cases of infectious bovine keratoconjunctivitis (IBK), however, the exact role of M. bovoculi in this disease remains unclear. Experimental Moraxella bovis (M. bovis) and M. bovoculi vaccines to prevent IBK based on recombinant pathogenic factors have been evaluated as alternatives to whole cell vaccines. Pilin is one candidate antigen for M. bovoculi, and is a key pathogenic factor necessary for attachment of M. bovis to the surface of the eye epitheliu...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

...ic Mastitis 

...

Sahar M. Saber1*, Ahmed M. A. Albehwar2, Amani M. Abas3, Omaima A.A. Alshamandy2, Noha A. El said4 and Marwa Y. Hamad4

...f interferon-induced by bovine ephemeral fever virus (BEFV) in Madin-Darby Bovine Kidney (MDBK) and African green monkey kidney (Vero) cell cultures against RVFV. Such interferon was successfully obtained with high concentrations by both cell cultures up to a dilution of 10 -5 yielding positive antiviral effect against RVFV, especially when inoculated one hour post virus infection or simultaneously with the virus. So, we cou...

Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2

...="text-align: justify;">Bovine herpesvirus-1 (BoHV-1) is globally circulated bovine virus of genital and respiratory importance. The present study was planned to investigate the sero-prevalance of BoHV-1 and its associated risk factors in District Sheikhupura, Punjab, Pakistan. The sample size was calculated using Raosoft sample size calculator. A total of 250 animals were sampled from herds of different sizes for sampling t...

Aqsa Batool1, Masood Rabbani1*, Farhat Nazir Awan2, Sohail Raza1, Sehrish Firyal3 and Shahan Azeem1

...="text-align: justify;">Bovine herpesvirus 1 (BoHV-1) is an emerging disease responsible for high production losses in the dairy animals. A cross-sectional study was conducted to determine the seroprevalence of BoHV-1 in and associated risk factors with disease prevalence in various agro-ecological zones of Pakistan focusing on the Punjab Province. A total of 400 samples were randomly collected from cattle and buffalo of wheat rice Punjab, mixed Punjab, cotton...

Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5 

...e detected in 50 random bovine skin samples. Each sample was kept in a separate sterile plastic bag and transferred to the lab in an insulated icebox for detection of the presence of ivermectin residues using the high-performance liquid chromatography (HPLC) technique. Skin histopathology and immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them 15 contained high ivermectin levels (100 ppb). Chlorpyrifos, ...

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

...llected from indigenous bovines of district Rawalpindi, Pakistan. The milk samples were screened for S. aureus and the confirmed isolates were subjected to disc diffusion test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S. aureus and MRSA-associated subclinical mastitis. The results revealed an overall molecular prevalen...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...="text-align: justify;">Bovine tuberculosis is a worldwide disease that causes great economic losses to the dairy industry and constitutes a serious human public health hazard. Development of a wide range of serodiagnostic assays for mycobacterium has been an area of interest through the past two decades as a rapid, accurate, and easy-to-be-used and interpreted diagnostic kit as well as to be a competitive alternative to the conventionally established expert-d...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...hich were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature oocytes which subsequently...

S.H.M. Faruk Siddiki1*, Md. Golam Morshed2, Md Robiul Karim1, Lutfun Naher1, Md. Sodrul Islam3

Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6

...cteria that involved in bovine respiratory infections. Its spreading problem is growing rapidly especially it could combine resistance with high-level of virulence traits that make it is more difficult to be treated. This study provides insight into the respiratory infections by Klebsiella spp. and its MDR profile in bovine cases, as well as the potential target genes that were involved in the links between virulence determi...

Asmaa A. Darwish*, Mona A. Mahmoud, Adel M. El-Kattan 

...erations associated with ovine and caprine brucellosis are a mirror for the immune response against the disease. Most of these changes were more prominent in goat than in sheep. TAC and Zn (separated or combined) are excellent biomarkers for ovine and caprine brucellosis.

Keywords | Brucellosis, Clinicopathological alterations, Matrix metalloproteinases, New biomarkers, Seroprevallance 

...

Safriyanto Dako1, Nibras Karnain Laya1, Syukri I Gubali1, Ari Ardiantoro2, V.M Ani Nurgiartiningsih3, Gatot Ciptadi3, Desinta Wulandari3, Suyadi Suyadi3*

...of 126 samples of local bovine blood were collected, which consisted of North Gorontalo (n=28), Gorontalo (n=52), Bonebolango (n=37), PO (n=3), and Bali (n=6), were used in this study. The Genomic DNA Mini Kits were used for DNA extraction for an analysis fragment in the microsatellite DNA region using ILSTS017, HEL13, and BM1818 primers. A total of 74 alleles were identified across entire populations. The expected heterozygosity ranged from 0.407±0.216...

Ahmed Abdel-Rady3*, Mohamed Karmi2, Menna_allah Youssef1, Aml M. Abdel-Ra’ouf1, Bahaaa Madkour1 

... and clinical status of bovine Theileriosis in Aswan governorate. During a 2-year study, 265 cattle were clinically suspected upon careful clinical examination as Theileria infected animals. Conventional diagnosis based on blood and lymph smears examinations showed that, the prevalence of Tropical Theileriosis in cattle in Aswan Governorate was 56 (21.13%). Giemsa stained blood smears showed presence of macro-schizont inside lymphocyte (Koch’s blue bodie...
Jameel Ahmed1,2*, Mohammad Masood Tariq1, Nadeem Rashid1, Asim Faraz3*, 
Majed Rafeeq1, Irfan Shehzad Sheikh1, Mudassar Jahan1,2, Abdul Fatih1,2
Masroor Ahmad Bajwa1, Ifrah Jameel1, Muhammad Ali1 and Saira Iftikhar4
...o genetic analysis using ovine molecular markers where fifteen sheep SSR genetic markers were employed for describing multiple-allelomorphism. The average observed number of alleles (Na), effective number of alleles (Ne) and Shannon’s Information Index were 2.07 ± 0.79, 1.86 ± 0.69 and 0.54 ± 0.29, respectively. Heterozygosity was described by the average, observed and expected heterozygosities whose values were found to be 0.56 &plu...

Fang Chen

...lemented with 10% fetal bovine serum, 100 µg/ml streptomycin and 100 U/ml penicillin and kept in humid air containing 5% CO2 at 37°C. The cell assay was performed when the cells were in the logarithmic growth phase. In the cultured H69/apatinib, the IC50 of apatinib was greater than 30nM, while it was about 13μM in H69 parental cells (P<0.05), indicating that the anti-apatinib SCLC cells were successfully constructed in vitro. The expression of...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3

...ypes Asia 1, O and A in bovines. A total of 838 bovine sera samples were tested through Solid phase competitive enzyme-linked immunosorbent assay (SPC-ELISA), based on structural proteins at the National Veterinary Laboratory (NVL), Islamabad from 10 January 2021 to 09 January 2022. The overall bovine population was carrying maximum seroprevalence of serotype O (28.52%) followed by serotyp...

Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*

...="text-align: justify;">Bovine Streptococcus are one of the main pathogens causing bacterial disease such as mastitis and endometritis in dairy farming. The virulence factors produced by Streptococcus are related to the occurrence of inflammation. To investigate the correlation between antimicrobial resistance and virulence traits of bovine Streptococcal isolates. Induced resistance was conducted for Streptococcus pneumonia ...

M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1

...) via TUNEL staining in bovine embryos produced in vitro from oocytes matured in stressful conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured under elevated levels of NEFAs for 24 h...

Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*

...l transcriptome between bovine embryos of different genders. In this study, we performed transcriptome analysis of cattle embryos based on a single Cell RNA-Seq. Bovine sex-controlled semen for artificial insemination were used to obtain different stage embryos: bovine 8 cell XX embryo, 8 cell XY embryo, 16 cell XX embryo, 16 cell XY embryo, morula XX embryo, morula XY embryo, blastocyst X...

Yakun Ge

...lemented with 10% fetal bovine serum, 50μunits/mL penicillin and 50μg/mL streptomycin, maintained at 37°C and 5% CO2 in a humid incubator environment. Quantitative analysis of ROS was performed using flow cytometry and median fluorescence intensity detection. Cell proliferation was detected by MTT method. Combined treatment promoted the production of ROS. The cell viability of the combined treatment group was lower than that of the control group at 2...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
...strains of S. aureus in bovine milk while metallic nanoparticles as effective alternatives.

...

Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1 and Iqra Nazir3

...="text-align: justify;">Bovine brucellosis is an important zoonotic and contagious disease targeting humans, domestic and wild animals all over the globe resulting in significant economic losses therefore, disease mitigation and control measures are of pronounced significance. The two available vaccines of Brucella abortus in Pakistan, RB51, and S19 have many limitations in the light of residual virulence, induction of abortion, diagnostic interference, and hu...

Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*

...tis vaccines containing bovine origin Staphylococcus aureus (tst), Streptococcus uberis (cpn-60 targeted STUB), and Escherichia coli (aggR) in a rabbit model. Four types of mastitis vaccines viz. formalin-inactivated plain polyvalent mastitis vaccine (FPPV), formalin-inactivated montanide adjuvanted polyvalent mastitis vaccine (FMPV), formalin-inactivated aluminum hydroxide adjuvanted polyvalent mastitis vaccine (FAPV), and formalin-inactivated montanide plus ...

Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li

...ious diarrhea caused by bovine viral diarrhea virus (BVDV), bovine rotavirus (BRV), and bovine coronavirus (BCV) have increased calves morbidity and mortality in Hebei Province. To detect these three pathogens simultaneously, we designed specific primers based on the conserved gene sequences of the three pathogens available in GenBank. After optimization of the reaction conditions and syst...

KHADIJA SUMMIA*1, ROHEELA YASMEEN*2, ALEEM UN NISA3, SAMIA DJEFFAL4 & TAHIRA JABEEN1

...ntify the aflatoxins in bovine feed provided to cattle found in and around Lahore. Total 50 samples were collected from two different localities of Lahore and categorized in two groups: Group A (Raiwind, 30 samples), Group B (Bedian Road, 20 samples). All samples were analyzed for estimation of aflatoxins level using thin layer chromatography (TLC) method. The results showed that out of 50 samples, 20 (40 %) were fit as no level of aflatoxins detected in these...

Putu Henrywaesa Sudipa1*, Hapsari Mahatmi1, Ketut Tono Pasek Gelgel1, I Gusti Ketut Suarjana1, I Nengah Kerta Besung1, Romy Muhammad Dary Mufa2 , I Wayan Sukernayasa3 

...tle. Earlier studies on bovine uterine microflora have mainly focused on bacteria; however, the presence of fungi has yet to be discussed in detail. This study aimed to provide data on the prevalence and identification of fungi by using morphological methods. The samples were vaginal swabs obtained from 22 healthy female Bali cattle from various locations in Bali, Indonesia. For isolation, vaginal swabs were cultured on Sabouraud’s dextrose agar (SDA) me...

Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2

...stance spectrum causing bovine respiratory tract infections in a large-scale cattle farm. Nasopharyngeal swabs were collected from animals (n=122) having clinical manifestations of respiratory tract diseases. Standard culture procedure followed by the Kirby-Bauer disc diffusion assay was applied to calculate the antimicrobial resistance profile of bacterial isolates. The survey results showed that bronchitis and bronchopneumonia were commonly occurring respira...

Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*

Arslan Muhammad Ali Khan1, Rao Zahid Abbas1*, Zia ud Din Sindhu1, Muhammad Shahid Mahmood2

...ainst Hyalomma ticks in bovines. Gas chromatography-flame ionization detection (GC-FID) was performed to identify the chemical components of A. subulatum essential oil. The acaricidal and repellent activities of the A. subulatum essential oil against Hyalomma spp. were observed via adult immersion test (AIT), the larval immersion test (LIT), egg hatchability test (EHT), and the tick repellency assay. GC-FID provided that the main compounds of A. subulatum esse...

Anas M. S. Al-Haj Afandi*, Ahmed H. F. AL-Bayati 

...e efficacy of acellular bovine tunica vaginalis and caprine acellular dermal matrix clinically and macroscopically, in reconstructing abdominal walls defects in Iraqi bucks. In 18 Iraqi bucks, ventro-lateral abdominal walls hernias measuring (6x6)cm were induced experimentally. Thirty days after creation of hernias, the animals were split randomly into two main equal groups (9 Bucks/ group). The hernias of group (A) were treated by sublay grafting of b

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

...="text-align: justify;">Bovine ephemeral fever (BEF) is an arthropode borne rhabdovirus, which causes a disabling febrile infection of cattle and water buffalo. A sever outbreak was recorded in Egypt during 2000 and 2001. Using RT PCR, a primer designed to amplify 438 nucleotide fragments from the glycoprotein G gene allowed specific amplification of BEFV-cell culture isolates from Egypt (Menoufia and Alexandria Governorates) and Japan. The PCR product was seq...

Ahmed Abd El Samie Hassan Ali

...="text-align: justify;">Bovine viral diarrhea virus (BVDV) is a world wide pathogen infects a wide variety of ruminants. A random 42 field pooled calf samples were collected and inoculated simultaneously intranasal, oral and intraperitoneal into 6 New Zeeland rabbits. The rabbits were observed for 25 day, slaughtered then tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for se...

El-Tarabili M. M., El-Shahidy M. S., Rifaat M. M., Kania S. A. and Abdelwahab Shahira A.

...oup of viruses including ovine herpesvirus-2 (OvHV-2), caprine herpesvirus 2 (CpHV-2) and white tailed dear strain (MCFV-WTD). Malignant catarrhal fever virus infection characterized by sporadic occurrence, fever and high mortality. In this study two real time PCR assays had been used to detect the MCF virus strains in both cattle and deer. The virus was detected in cattle samples by Ovine MCF RT-PCR while was detected in de...

Hassanein, S.A.*; Ibrahim, A.K**; Mervat, M.M.* and   Nahed,K.A.*

...sons, 3 main strains of bovine herpesvirus (BHV) were defined. These strains were (I) Abu-Hammad strain and Colorado strain, (Il) Cattle isolate and Goat isolate and (Ill) sheep isolate. In conclusion, virus neutralization test could not differentiate bovine herpesvirus wpe I (BHV -l) from other strains of herepesviruses; on the contrary, RFLP and RAPD assays could be used to differentiate the virus. Thus, both techniques ca...

JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.

...ully isolated from three ovine samples after three successive passages on MDBK•cells. The isolated viruses were then titerated and identified using different biological, serological and molecular assay.

...

Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.

...GIO serotype of group A bovine rotaviruses ranging from 90-93%. This is the first report on the circulation of G 10 serotype of group A rotaviruses in camel. 

...

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

...zation antigen (VP7) of bovine romvirus Nebraska calf diarrhea virus (NCDV) strain in insect cells. The full-length DNA copies of RNA segment 9 (coding for VP7 protein) of NCDV was inserted into a baculovirus transfer vector under the control of the polyhedrin promotor. A recombinant baculovirus carrying the VP7 gene was constucted through homologous recombination between the baculovirus transfer vector carrying the VP7 gene and Autographa californica Nuclear ...

Attyat, M. Kotb; Abeer, E. Mansour; Naglaa, I. Aly; Zeinab, T.Salama and Azab, A. Mohamed

...ation of cattle against bovine ephemeral fever (BEF) and bivalent foot and mouth disease (FMD) strain O and strain A. so fifteen calves, six month old, were vaccinated singly and simultaneously and two calves were left as none vaccinated control. The serum neutralizing antibody titer expressed in log10 of FMD (strain O and strain A) in calves vaccinated with bivalent FMD vaccine only were increased from (0.9 log10 and 0.9 log10) consequently at the first week ...

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

...g and expression of the bovine coronavirus (BCV) nucleocapsid protein was carried out in a baculovirus expression system. The specific RT-PCR product of N gene was cut and extracted from gel using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that ...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...showing signs suspected bovine ephemeral fever (BEF). Blood samples on anti-coagulant, nasal swabs and tissues (bovine liver, kidney, lymph nodes and lung) for BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on b

M.A. Farag*, Abeer, E. Mansour* and s.M. 

...justify;">The effect of bovine viral diarrhea virus (BVDV) infection and Aflatoxine BI (AFBI) on the immune response of sheep vaccinated with bivalent Foot and mouth disease (FMD) vaccine were studied. Significant decrease in humoral immune response against the vaccine strains 01/3/93 and A/l/Egypt/2006 accompanied by protection percentage of 33% against challenged virus 01/3/93 with low values of AOD were recorded in sheep previously infected with BVDV one we...

M.R. Abd-El Wahab l, H.A. Hussein2, T.M. Asfourl and M.A. Shalaby2

... from those reported in bovine and ovine populations. 

...

Ahmed Abd El-Samie H. Ali

...="text-align: justify;">Bovine viral diarrhea virus (BVDV) is a worldwide pathogen infecting cattle. Eleven Holstein calves between 6-8 months of age that were seronegative to BVDV were divided into three groups (n=4 for NY-1, n=4 for 890 and n=3 control). Fibroblast cells were prepared from calves' skin biopsies by trypsin digestion. Using immunofluorescence. the percentages of infected cells and MHC antigens expression were evaluated. fibroblast and peripher...

S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3

...="text-align: justify;">Bovine ephemeral fever virus (BEFV) is an arthropod born rhabdovirus which causes disabling febrile infection among cattle and water buffalo. The disease is characterized by inflammation of mesodermal tissue as achieved by muscular shivering, stiffness, lameness, and enlargement of the peripheral lymph nodes. Recently, the disease caused several outbreaks during 2000, 2001 and lastly 2004. The persistence of immunity after natural infec...

Musaad A. Al-Dubaib

...regions were tested for bovine leukemia virus (BLV) antibodies for different animal species. Agar gel immunodiffusion was carried out using commercial diagnostic reagents. All samples of herds having positive cases with immunodiffusion were tested by ELISA. Positive cases were recorded among samples of the Holstein cattle with both serological tests. Prevalence rates differed from one farm to another and with both serological tests. With agar gel immunodiffusi...

Arwa H. El -Naggar, Nirmeen G. Shafiek, and M. S. Wassel.

...er immune serum against bovine Rota was prepared in guinea pigs through its inoculation with complete and incomplete Freund's adjuvant and without adjuvant in successive doses into susceptible guinea pigs. Serum neutralization (SNT), ELISA and indirect fluorescent assay (IFA) tests were used to evaluate the hyper immune serum Before conjugation with fluorescent. The antibody titer reach 32 by SNT and 5984 by ELISA and identified by IFA to be B

Ausama A. Yousif1,3, Sandy M. Watkins1, Lyle J. Braunl, David J. Hurley2 and Christopher C. L. Chase1

 

...and vaccinal strains of Bovine virus diarrhea virus (BVDV) indicate that mixed pestivirus infections occur in vivo and that bovine cells support replication of RNA from BVDV-I and BVDV-2 (Ridpath and Bolin. 1995: Becher et al. 2001). BVDV type-specific molecular beacons were used to estimate the effect of the presence of two BVDV RNA genotypes on the RNA relative replication efficiency of each genotype by comparing Ct values...

Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2 

...n.
 

...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...sma spp. infection in a bovine host using both microscopy and molecular techniques. A total of 59 samples were collected from Mingaladon township, Yangon region, and the presence of infection was assessed using a Giemsa-stained thin blood smear and PCR of 16S rRNA gene amplification. Both microscopic and PCR-positive samples were further analyzed for hematobiochemical alteration. As a results, Anaplasma spp. was detected in 3.38% (2/59) of sampled animals. Hem...

Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7

...anulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group withou...

María Julia Traversa1*, Mónica Saracco2, María Alejandra Colombatti Olivieri3, Fernando Paolicchi4, Edgardo Rodriguez5, Silvia Estein5, María Cristina Jorge5, William C. Davis6

...ows that reacted to the bovine purified protein derivatives (PPDb) caudal fold skin test and were transiting the early peripartum period (EPPp). Flow cytometry, interferon gamma (IFNγ) production, and metabolic activity assessed peripheral blood mononuclear cells (PBMC) stimulation. The study enrolled 19 Argentinian Holstein cows older than two years classified into four groups, one of PPDb reactors that transited the EPP period (PPDbEPPp) (n=5), another...

Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2

...dard 4869-1:2021 frozen bovine semen. 
 
Keywords | Cryopreservation, Myrmecodia extract, Tris egg yolk, PO bull, Tannin, Protection
...

Mercy Cuenca-Condoy1*, Lourdes Reinoso-García2, Juan González-Rojas2, Dionel García-Bracho3  

... that cause subclinical bovine mastitis and their frequency in the Biblián-Ecuador canton. One thousand four hundred and forty mammary quarters were analyzed, coming from 360 Holstein cows in milk production. The California Mastitis Test (CMT) was used to qualitatively verify the increase in somatic cell count (SCC) in milk to indicate the presence of microorganisms causing bovine mastitis. Of the 1440 quarters studi...

Ahmed Kareem Kadhim Al-Wasmee1, Waddah Salam Hassone2, Hawraa Talib Al-Janabi3, Hamed AH Al-Jabory1

...ropical theileriosis in bovine herds and causes significant economic losses in the cattle sector. We estimated and interpreted the frequency of theileriosis in the calf herds in Babylon Province. We carried out the study from July until the end of December 2022 in several regions of Babylon Province. Ninety jugular vein blood samples were collected. The calves ranged from <1 M to ≥4 M and both sexes were represented. Two ml of blood were placed in steril...

Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1

...rain for the control of bovine brucellosis but has several reported drawbacks including pathogenic potential for humans and excreted in body fluids of vaccinated animals. In the present study, we constructed a more attenuated B. abortus ΔpurD mutant by deleting the purD gene from the RB51 strain through site-directed mutagenesis. For virulence attenuation comparison with the parent RB51 strain, the constructed mutant was evaluated for attenuation estimat...

Aya A. Saber1, Mohamed Fawzy2*, MokhtarM. EL-Tarabili2, Eman K. El Sayed2

...tense formerly known as Bovine ephemeral fever (BEF) and Rift Valley fever (RVF), respectively, are insect-borne viral diseases that affect cattle, causing significant economic losses. The application of a promising vaccination strategy requires the utilization of safe and effective vaccines capable of successfully preventing and controlling these disastrous diseases. The current study explored the effect of simultaneous vaccination of cattle with BEF and RVF ...

Karin Alvåsen1*, Karin Maria Olofsson2, Raymond Kent Fenton3, Ulrika Rockström4, Lena-Mari Tamminen1, Nils Fall1

...m. Images are sent to a bovine practitioner, experienced in interpreting gross lesions, for analysis. In this pilot study, education and training sessions on the RDN method were administered to veterinarians. After the training sessions, the participants performed 15 RDNs. Subsequently, interviews were conducted with 10 participants to capture their experiences and views on using the RDN method. In many of the necropsied animals, the RDN diagnosis matched well...

Tebogo Letsukulo Percy Thepa, Thobela Louis Tyasi*

... the association between ovine growth traits and the SNPs of the MSTN gene. Four databases (PubMed, Web of Science, ScienceDirect and Google Scholar) were systematically evaluated using the keywords sheep, MSTN, polymorphism, genetic variation, growth traits, gene polymorphism, skeletal muscle growth, GDF-8, without any year of publication restrictions and 8 studies were found to be eligible. The results indicated that eight (n = 8) publications which were exa...

Ghalib Nadeem* and Muhammad Irfan Anis 

...="text-align: justify;">Bovine disease identification utilizing multiple information sources and methods is widely applicate in the field of bovine disease prevention and health monitoring. Bovine disease detection is an emerging subject today to accomplish the farms demands of individuals across the globe. This research delves into the realm of bovine d...

Safdar Abbas1, Jawaria Ali Khan1*, Syed Saleem Ahmed1 and Aftab Ahmed Anjum2

...="text-align: justify;">Bovine coronavirus (BCoV) is a major viral pathogen linked to respiratory and enteric problems in newborn calves. The goal of this study is to look into the molecular occurrence, haemato-biochemical changes, and risk factors associated with the occurrence of BCoV infection in cattle calves at different dairy farms and small households in the district of Jhelum, Pakistan. From July 2020 to June 2021, 200 faecal samples were collected fro...

Abdul Rahman Sesay1,2, Muhammad Saif-ur-Rehman1*, Faisal Ramzan1 and Faisal Saeed Awan3

...was conducted using the BovineHD140k BeadChip. After undergoing quality control measures, 87 samples as well as 74,070 SNPs located throughout 29 autosomes were selected as well as included in the study. The selection signatures were examined using the iHS and the Tajima D approach. The result reveals the current positive selections on BTA 1, 2, 6, 11, 12, 15, 17, 21, and 27 with the iHS test, while for the Tajima D test, the current positive selections were d...

Helal F. Al-Harthi1*, Essam H. Eldrehmy1,2, Afaf Eladl3, Marwa I Abd El-Hamid2*

...ples were obtained from bovine meat (n= 30) and meat products (n= 120) samples in Sharkia Governorate, Egypt. Conventional identification techniques detected 50 (33.3%) staphylococcal isolates (32.8%); 28 (18.7%) were classified as S. aureus and 22 (14.7%) were identified as coagulase-negative staphylococci (CoNS). All the examined S. aureus isolates were resistant to oxacillin and cefoxitin. Meanwhile, the lowest resistance patterns were detected against chlo...

Md. Nazrul Islam1*, Md. Saiful Islam Siddiqui2, Md. Rafiqul Islam3, Emdadul Haque Chowdhury3’ 

...egligible.  

...

Tanveer Hussain1, Abdul Wajid1, Jabbar Khan2, Asif Nadeem1, Misbah Hussain1, Qurat ul-Ain1 and Masroor Babar1*

...lified by using reported ovine IL-2 primers. Amplified products of 4 breeds of goat were bidirectionally sequenced to decipher polymorphisms. Only a single substitution (T→A) was found in non-coding region of IL-2 gene. Comparison of IL-2 gene sequence of all four breeds with other goat breeds showed high similarity in sequence. Phylogenetic analysis of our local breeds with other mammals showed that IL-2 was highly variable. This high substitution rate c...

Hafizuddin1, Sri Wahyuni2, Tongku Nizwan Siregar1*, Amalia Sutriana3, Ririn Triyuliani4, Siti Hanyyah Herviani4, Muhammad Farhan Syuhada4, Nur Salimah4, Muharni Mardiah Eriswandi4, Ratu Permata Ayu4, Putroe Hayatun Nufus4

... recommended the use of bovine pituitary extract (BPE) as an effective alternative in improving animal reproduction. Therefore, this study aims to determine the ovarian and hormonal responses of local rabbits after superovulation induction with BPE. The sample comprised of 1 local male rabbit and 9 local female rabbits with a history of giving birth and weighing between 1.8-2.2 kg. The animals were adapted for 30 days in separate cages and provided with feed a...

Alain Kouam Simo1, Roland Nankam Chimi1*, Stevine Ngoueni Megouo1, Ariane Megne Fossouo1, Félicité Flore Djuikwo-Teukeng2, Mamoudou Abdoulmoumini3

...ce and risks factors of bovine schistosomiasis in the North, Adamawa and west, regions of Cameroon. A total of 700 cattle were examined in slaughterhouses in the Adamawa, North and West regions from February to September 2023.Screening methods by visual exploration of the mesenteric veins in search of adult worms and coproscopic analysis using the filtration technique were used. This last technique, although being the technique of choice because it is done on ...

Israa M. Essa*, Ghazi Y. Azzal, Alaa Tariq Abdulwahid

...phylogenetic analysis of ovine liver flukes indicated worthily that F. hepatica was the more prevalent Fasciola species in sheep of Basra province. In addition, our results provided an initial basic data for monitoring this potentially important parasite in field. However, the main limitations of the present study include the low number of examined animals, short period of study, and disapproving of some owners to examine their slaughtered animals. Therefore, ...

Mokammel Hossain Tito, Most Hoor E Jannat, Marzia Afrose, S.M. Jubayer Ahmed, Shah Md Maruf, Md. Arafat Hossain, Safiullah Samani, Ruksana Jahan Mira, Barshon Saha, Asraful Islam Jihad and Tonmoy Kumar Das

... dataset comprising 266 bovine sera samples collected from Diga, Guto Gida, and Nekemte districts, employed machine learning (ML) algorithms to predict FMD outbreaks and assess attribute correlations. The dataset is taken from mendely with prevalence ranges from 4.8% to 72.1% in cattle. In this paper we have used total of five algorithms including Naïve Bayes, MLP (Multilayer Perceptron), SMO (Sequential Minimal Optimization), AdaBoostM1, and REP Tree. Ea...

Eman Mamdouh Qenawy1, Mohamed Abou-Ellail1, Fatma Abdel-Motaal2, Mohammed O. Alshaharni3, Nady Khairy Elbarbary4*

...cterial count than pure bovine meat. P. aeruginosa and E. coli were identified in 41.1% and 30% of the examined samples, with a high occurrence rate in minced beef samples. All P. aeruginosa isolates examined by PCR carried the rpoB gene, and 70% and 40% showed positive lasB and exoS virulence genes. Meanwhile, E. coli isolates showed hylA, stx1, and stx2 virulence genes in 50%, 80%, and 20% of them. Furthermore, this investigation’s PCR technique detect...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
... a highly transmissible bovine disease caused by a virus belongs to Poxviridae family (genus Capripox). The disease was originally isolated from cattle in Egypt in 1988 and it later became widespread in the most of governorates. Because of the appearance of vaccine-associated disease recently, it is critical to differentiate infected from vaccinated animals (DIVA) strategies. Therefore, the aim of this study was to detect LSDV in suspected clinically diseased ...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
... a highly transmissible bovine disease caused by a virus belongs to Poxviridae family (genus Capripox). The disease was originally isolated from cattle in Egypt in 1988 and it later became widespread in the most of governorates. Because of the appearance of vaccine-associated disease recently, it is critical to differentiate infected from vaccinated animals (DIVA) strategies. Therefore, the aim of this study was to detect LSDV in suspected clinically diseased ...

Imtiaz Khan1, Arsalan Khan2*, Umme Aimen2, Waseem Ullah2, Akhtar Ali2, Qamar Ullah3, Muhammad Faimullah Khan4, Abdul Wadood Jan5, Salma Zaman6, Abdul Qadoos1, Tayyeb Ullah7 

Hind Abdulzahra Abdulkadhim Alshaibani1*, Abdul Jaleel Aziz Karim Alqaraghli2, Mohammed Azeez Yasir Alzaidi3 

...s, as the prevalence of bovine fasciolosis in females and males reached 15% and 9.09%, respectively. The infection rate in slaughtered buffalo for males and females was 13.33% and 20%, respectively. The results of the current research also showed that the infection rate in the age group <1.5 reached 10.34%, while the infection rate in the age group 1-2.5 was about 13.79%, and the infection rate in the age group 2-2.5 was about 15. 51%. In the age group betw...

Faheen Riaz1, Sarfraz Mehmmod1, Aatka Jamil1, Khansa Jamil1, Imran Riaz Malik2, Muhammad Naeem Riaz1* and Ghulam Muhammad Ali1

...protein produced by the bovine CYP11B1 gene, which has nine exons and eight introns and is located on BTA14q12, is linked to the mitochondrial epithelium. The CYP11B1 gene is located close to the marker ILSTS039, this marker is connected to milk characteristics. This makes the CYP11B1 gene, along with milk yield and milk component yield, the functional and positional candidate gene for the milk production traits. 

...

Hayder A.H. Al-Mutar*, Baqer J. Hasan, Khawla Abbas Hussein, Souhayla Oneeis Hussain

...st bacterial present in bovine vagina were Streptococcus thermophilus and Streptococcus uberis (92.65%) while the lowest percent of bacteria present in bovine vagina was Corynebacterium frankenforstense (78.8%). The other saprophytic bacteria were Streptococcus lutetiensis, Klebsiells pneumoniae, Escherichia coli and Staphylococcus aurreus were present in a percentage from 94.61%, 93.25%, 80.5% and 78.69%. respectively. The ...

Oula E. Hadi*, E.H. Altaee

...) is being evaluated in bovine kidneys, a tissue in which the toxic effects of this metal have been demonstrated. The study was conducted on several groups of grass-fed cows in different locations in Basra/Iraq. Histopathological examination of the kidneys revealed hemangiomatous transformation in cows with U in the kidney. Uranium levels were measured using a sodium iodide device, and samples were taken from the cows’ kidneys, as they are the organs mos...

Mohammed A. Hamad1*, Murtadha Abdule Mahdi Al-Mudhafar2, Alaa Fayeq Habeeb3, Firas R. Jameel1, Ismael Raheem Al-Muhana2, Najemaddin A. Hamad5

...="text-align: justify;">Bovine pneumonia is a complex disease which results in significant financial losses. The aims of this investigation were to identify the respiratory syncytial virus, the prevalence of infection that has spread throughout Al-Anbar region, and the factors that contributed to the infection in Calves also by detecting the virus using a molecular technique. In this study, fifty-two (n=52) nasal swabs were collected from male and females in y...

Journal of Animal Health and Production

November

Vol. 12, Sp. Iss. 1

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