Effect of Cortisone and Cortisol on Prostaglandins Production by Bovine Endometrium Around the Time of Ovulation
Effect of Cortisone and Cortisol on Prostaglandins Production by Bovine Endometrium Around the Time of Ovulation
Duong Thanh Hai1*, Tomas J. Acosta2, Tran Thi Minh Tu3
ABSTRACT
This study was conducted to examine whether cortisone (Cs) has the capacity to regulate prostaglandin E2 (PGE) and prostaglandin F2alpha (PGF) secretions by bovine endometrium around the time of ovulation. In preliminary experiment, doses of PF915275 (PF, 11-β hydroxysteroid dehydrogenase type 1 inhibitor) was exanimated. PF at a dose of 1000 nM (PF1000) decreased significantly the absolute conversion of Cs to Cr (p<0.05). Thus, the PF1000 was chosen for the further studies. Tissues of endometrium were collected at the follicular stage (days 19-21), and exposed to Cs (300 nM) or Cs (300 nM) combined with oxytocin (OT, 100 nM) in the presence or absence of PF1000 in 4 h. The PGE and PGF secretion in cultured endometrial tissue did not affected by Cs and Cr converted from Cs. PGE and PGF production regulated by OT was higher in the presence than in the absence of PF1000 indicating that PGE and PGF production stimulated by OT was suppressed by Cr converted from Cs. The overall results suggest that Cr converted from Cs, but not Cs, has the capacity to regulate PGE and PGF production in bovine endometrium at the follicular stage.
Keywords | Cattle, Cortisone, Cortisol, Ovulation, Uterine function
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July 2022
Vol. 10, Iss. 7, Pages 1423-1658
