Mudasir Irfan Dar*, Fareed Ahmad Khan and Farha Rehman

E-mail | irfanmudasir@gmail.com

...gments and antioxidative enzymes in Brassica juncea L. cv. alankar was investigated. Forty-day-old pot plants were exposed to different concentrations of the insecticide, ranging from 0 to 40 grams active ingredient per hectare (g.a.i ha-1) through foliar spray. Analyses were done at days 3, 7, and 15 after treatment. Lipid peroxidation rates and contents of proline, ascorbate (ASC), glutathione (GLU), antioxidative enzymes;...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...activity of antioxidants enzymes because of polymorphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A total of 400 indiv...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...ge and diverse family of enzymes with a wide phylogenetic distribution. They are multifunctional enzymes that play a crucial role in cellular detoxification and oxidative stress tolerance. Comparing with that in mammals, investigation of GSTs is more complicated in teleosts because of the greater pressure they suffer in aquatic environment. In this study, we identified a set of 27 GSTs including 8 classes of members in commo...

Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3

...reserved for antioxidant enzymes assay analysis. The data showed that as the citrus waste percentage in the feed of the broiler was increased, its feed intake and the body weight decreased but the citrus waste has non-significant effect on the feed conversion ratio of the broiler birds. The results also showed that citrus waste in the feed have positive effect on the antioxidant status of the broiler birds. The activity of the superoxide dismutase, catalase an...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

...d effects on antioxidant enzymes i.e. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in vital organs (gills, liver and kidney) of fish, Cirrhina mrigala. The 3 different representative water samples and 60 fish samples (20 fish from each site) were collected from three sites i.e. fish-farm, hatchery and Indus River of Punjab, Pakistan. The results showed differences in physico-chemical parameters of water samples collected...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
... other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology with the porcine GSTpi enzyme. Gene expression analysis in five camel tissues was examined employing real-time PCR. The highest level of transcripts was found in the camel testis,...
Tanzeela Riaz1, Farah Rauf Shakoori2,* and Syed Shahid Ali3
... phosphine on microsomal enzymes (NADPH-Cytochrome P450 reductase (NADPH-CPR), ethylmorphine-N demethylase and aniline 4-hydroxylase) and soluble protein contents of a wheat grain pest, Trogoderma granarium collected from godowns of Punjab, Pakistan. Six populations of khpara beetle with different levels of susceptibility to phosphine were used in this study. Based on LC50, five populations (previously exposed to phosphine for 15 y...

Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3 

...berine toxicity on liver enzymes, renal function, serum uric acid and blood cell counts of Sprague-Dawley rats. For this purpose, twenty Sprague-Dawley rats were divided in two groups. Group A (n=10) was given distilled water and Group B (n=10) was supplemented berberine hydrochloride with dose of 75 mg/kg body weight/day. 24 weeks post-treatment, blood samples were collected by cardiac puncture from both groups and tested for Hb (%), WBC, RBC and platelet cou...
Saba Rafique1,2,*, Khalid Naeem1, Naila Siddique1, Muhammad Athar Abbas1, Aamer Ali Shah2, Akbar Ali1, Abdul Rahim1 and Farooq Rashid1
...racterized by RFLP using enzymes Alu I, Hae III, BstYI and XcmI. On the basis of its distinct RFLP pattern from other known IBV vaccine strains, the isolate was named as KU145467_NARC/786_Pakistan_2013 (also named as Pak-786) was subjected to Spike gene sequencing covering the amino-terminus region of 01 to 974 base pairs. The S protein sequence was submitted to the GenBank with accession number KU145467. Phylogenetic grouping and maximum nucleotide sequence i...
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2

 

...ta indica on the CNS enzymes of a stored grain pest, Tribolium castaneum and a socioeconomic insect, Apis mellifera. A comparative study was designed to identify the role of saponins on insect acetylcholinesterase (AChEs). The enzyme activities were tested for the effect of saponins. The AChE activity of T. castaneum was inhibited by the saponins and follows competitive inhibition kinetics. In case of A. mellifera enzyme activit...

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...ces in the activities of enzymes and quantities of total protein, soluble protein and FAA between strains and among concentrations. Abamectin, at LC and LC , changed the activities 10 20 /levels of TE, CE, AcP, total protein and FAA in the larvae of both the strains. The activities of alpha-amylase, glucoamylase and AkP remained non-significant at both doses in the two strains. In PAK strain larvae, the TE activity was inhibited with depletion of total protein...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...icrobial species utilize enzymes as virulence factors to cause diseases. The aim of the present study was to evaluate enzymatic activities of the bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, val...
Fouzia Tabssum1* and Syed Shahid Ali2
...Microbial pectinases are enzymes of industrial importance; they are being used commercially in a variety of ways such as clarification of the juices in beverage industries, tea making, and wastewater treatment among others. The present investigation was undertaken to isolate pectinase-producing bacteria from local habitats. To enrich the diversity of isolated bacteria that produce pectinase, samples were collected from the soil, water, fruits and vegetables wa...
Fouzia Tabssum1* and Syed Shahid Ali2
...Microbial pectinases are enzymes of industrial importance; they are being used commercially in a variety of ways such as clarification of the juices in beverage industries, tea making, and wastewater treatment among others. The present investigation was undertaken to isolate pectinase-producing bacteria from local habitats. To enrich the diversity of isolated bacteria that produce pectinase, samples were collected from the soil, water, fruits and vegetables wa...
Leila A. Kaimbayeva1,*, Elena S. Malysheva2, Rashit Kazikhanov3 and Saule R. Kazikhanova4
...er, activities of tissue enzymes and microstructure changes. The results of chemical analysis revealed the nature of protein substances, altered рН value of the meat and the intensity of glycolysis. All these parameters were indicative of autolysis process in the meat. The levels of water-binding capacity and structural-mechanical properties of red deer meat that occurred during the course of autolysis were further confirmed by the histological cha...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...arbohydrate metabolizing enzymes, the activities of amylase and invertase were significantly reduced while activity of trehalase were significantly increased after treatment with sub lethal dose of λ-cyhalothrin as compared to control. The metabolic derangements induced by sub-lethal dose of λ-cyhalothrin suggest that infestation caused by T. granarium in godowns could be overcome by calculating lethal dose of λ-cyhalothrin.
...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,* 
... in level of antioxidant enzymes and significantly increase in the MDA contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parameter and decrease in MDA contents significantly (P<0.05). SM treatment of TD-afflicted birds prevented lameness and reinstated antioxidant imbalance. SM may be...
Tayfun Karataş
...abolites and antioxidant enzymes, thiobarbituric acid reacting substance levels, endogenous reserves in liver and muscle tissues of rainbow trout (Oncorhynchus mykiss). Eight-days of fasting caused a significant decrease in glucose, total protein, triglyceride, cholesterol, high-density lipoprotein and low-density lipoprotein levels as well as protein and lipid reserves in liver and muscle tissue of fish (p<0.05). The fasting period had no significan...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...arbohydrate metabolizing enzymes, the activities of trehalase, amylase and invertase were significantly reduced after treatment with sub lethal dose of esfenvalerate as compared to control. The metabolic derangements induced by sub-lethal dose of esfenvalerate suggest that infestation caused by T. granarium in godowns could be overcome by calculating lethal dose of esfenvalerate.

 

...

Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil

Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
... increase cardiac marker enzymes. Treatment of rats with GFAE (45 mg/kg/day) prior to injection of ISO provide significant cardio-protective effects evidenced by an obvious reduction in the level of cardiac marker enzymes, inflammatory factors and lipid contents with marked improvement in the cardiac antioxidant status and reduction of lipid peroxidation relative to untreated infarcted group. The study concluded gamma...
Asima Bano1, Hafiz Muhammad Tahir2, Hira Sherawat3, Muhammad Mutlib4, Muhammad Arshad5, Muhammad Akram Qazi6, Sajida Naseem5, Rabia Ishaq1, Iram Liaqat2 
...ssociation of two stress enzymes i.e., monooxygenases and glucathione S-transferases with breast cancer. For enzyme estimation plasma samples from the patients suffering from breast cancer and age matched healthy individuals (control) were collected. The results revealed decline in the level of these enzymes in breast cancer patients as compared to healthy individuals because of this decline the overall level of protein was ...

 Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2

Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
...he activity of digestive enzymes was determined in Hypophthalmichthys molitrix, Catla catla and Cyprinus carpio fed
with different feed ingredients. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied significantly ...

 Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3

Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...ase in levels of hepatic enzymes AST and ALT in blood plasma of exposed fish as compared to control fish.
These results suggested that deltamethrin is highly toxic for aquatic organism like silver carp causing malfunctioning
of liver.

...

 Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1

Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...nges in the liver marker enzymes along with the change in the sera and tissue total
protein contents specifically albumin.

...
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...erum activities of liver enzymes, level of hepatic and testicular lipid peroxidation and plasma levels of caspase 3 associated with significant increase in the levels of luteinizing hormone, follicle stimulating hormone and testosterone in addition to marked improvement in the level of total antioxidant capacity and glutathione content and the activity of superoxide dismutase activity and catalase activity of the liver and testes of lead-intoxicated rats. In c...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
...pplementation of phytase enzymes affected the weight and length of small intestine and feed conversion ratio (FCR) and did not affect the other zoo technical variables like body weight, feed consumption, and weight gain during the whole experimental period. Application of phytasefailed to exert any influence on the weights of gizzard, provetriculus, heart, liver, spleen and empty intestine. The weights and lengths of the small intestine were highly significant...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
...of the activities of the enzymes such as ALAT (alanine aminotransferase), ASAT (aspartate aminotransferase) and LDH (lactate dehydrogenase) and biochemical components like glucose, urea, lipids, cholesterol and protein contents both in the liver and blood while DNA and RNA contents only in liver. The administration of CCl4 resulted in increase in plasma ALAT and decrease in LDH. Vitamin E pre-treatment abolished CCl4-induced changes in th...

Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1

Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
...in production of various enzymes and proteins in unconventional and economical substrates suitable for various industrial uses.
...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2, Wardah Hassan3, Moazama Batool4, Shakeela Perveen5, Sadia Maalik4 and Sajida Mushtaq4
... activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different organs (liver, gills, kidney, brain, heart and muscle) of fish, Labeo rohita. The LC50 of chlorpyrifos+endosulfan mixture was calculated as 1.95±0.02 μgL-1 for 96 h with the 95% confidence limits. The fish expose to the mixture (1:1) for 96-h. The results obtained from this study showed that superoxi...
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan, Wen Lu and Xialin Zheng*
...toxifying and protective enzymes in P. flammans larvae were also measured. Results showed that the LC50 value of beta-cypermethrin, abamectin, chlorpyrifos, thiamethoxam and bisultap against P. flammans larvae were related to the instar and pesticide. Control efficacy of beta-cypermethrin was the best among of these pesticides, and the LC50 value of which against 1st instar larvae in P. flammans reached 2.0...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...al parameters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily ration feed was 4% of the body weight. ...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
... activity of antioxidant enzymes in fish. T
hese parameters can be used as a good biomarker to evaluate the aquatic pollution.
...
Muhammad Naveed Sheas1, Haris Rasool2, Muhammad Nouman Rafique2, Muhammad Rizwan Tariq1*, Abid Muhammad1, Khubaib Ali1
...one in which antioxidant enzymes like SOD, Catalyze, Glutathione peroxidase were performed that shows significantly high levels in the blood than the group that was treated with nicotine and shows a positive correlation with the days at which the rat’s groups were given a supplemented feed. Total antioxidant capacity and antioxidative stress shows significant results by the selected treatment. 
...
Zeynep Soyer Sarıca1*, Meryem Eren2 and Meryem Senturk2
...ione peroxidase (GSH-Px) enzymes were estimated in the brain tissues of the Sprague Dawley rats. Compared to the control groups, a significant increase in the brain tissue MDA level of the group which received only 10 mg/kg K2Cr2O7 was observed, whereas no statistically significant change was detected in SOD, CAT and GSH-Px enzyme activities. It was further observed that after administration of Boron at 5 and 10 mg/kg the lipid...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...ction of three different enzymes (alpha-amylase, cellulase and pectinase) from aqueous system has been studied by applying novel concept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen m...

Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3

...on biomass and different enzymes viz; xylanase, CMCase and laccase production by Pleurotus ostreatus were optimized in shake flask cultures. The results indicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum l...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...ctivities of antioxidant enzymes. These enzymes are effective in protecting the bio-molecules against the reactive oxygen species (ROS) and their harmful effects. This study was aimed to evaluate the effects of sub-lethal concentrations of manganese on antioxidant activity in the fish, Labeo rohita. Fish were acclimatized for two weeks in the cemented tanks prior to the start of experiment. Four groups (n=10) of one y...

Nosheen1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1 

...cluded that antioxidants enzymes can be used as a valuable biomarker of oxidative stress in aquatic animals. 

...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
... to hydrolytic family of enzymes which occupies a great commercial position because of their high stability under alkaline and acidic conditions. The current research was planned to isolate, screen and optimize mannanase production from Bacillus megaterium from decayed fruits. Microorganisms were isolated from fruits, soil and water samples obtained from different localities of Lahore city, Pakistan. Locally isolated microorganisms were screened for the...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...creased. Levels of serum enzymes AST and ALT increased with ingestion of dietary AFB1. It is concluded that ingestion of very low level of AFB1 by lactating goats results in excretion of carcinogenic metabolite AFM1 in milk beyond the permissible level. Dietary AFB1 has role in sub-clinical mastitis and causes injurious effects on general health status of lactating goats.
...
Fariha Latif1*, Muhammad Javed2, Hammad Ahmad Khan2 and Khalil-Ur-Rehman3
...ctivities of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). The Pb exposure caused a significant time and dose dependent increase in the SOD and POD activities in selected tissues of C. mrigala while a significant decrease in the CAT activity was also observed. Liver showed a significantly higher activity of all the three enzymes that could be due to its actively metabolic na...
Saman Muhsin Abdulkareem* and Nadir Mustafa Nanakali
... oxidative stress, liver enzymes activity and the expression of cytochrome P450 1A1 (CYP1A1) against hepatotoxicity induced by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in male rat. Thirty adult male Wistar rats randomly divided into five equal groups. TCDD and QCT were orally administered at the dose of 10µg/kg /day and 20 mg/kg/day, respectively by gavage dissolved in corn oil for 90 days. At the end of the study, animals were sacrificed and...

Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2 

...induction of antioxidant enzymes. This study was planned to evaluate the organic elicitors and to determine the biochemical changes in citrus fruit. 

...
Derya Kocamaz* and Elif Oruc
... activity of antioxidant enzymes was highest and the level of hormones was lowered. The group of the mixture pesticide showed the highest lipid peroxidation and protein carbonylation than only thiamethoxam or λ cyhalothrin exposure. In conclusion, the mixture of the insecticides showed a more toxic effect on the gonad of tilapia compared to individual pesticide exposures. In addition, the depuration period of 7 days was not adequate to eliminate the tox...

Ahmad Naeem Shahzad1, Muhammad Kamran Qureshi2, Samee Ullah1, Muhammad Latif3, Shakeel Ahmad1 and Syed Asad Hussain Bukhari1* 

...ctivities of antioxidant enzymes (superoxide dismutase, peroxidase and catalase) in plant leaves were increased in response to salt stress. Treatment of stressed plants with trehalose considerably improved the growth attributes and reduced the activities of antioxidant enzymes, indicating its ameliorative role in salt stress alleviation. However, no improvement was noted in K+ and Na+ dynamics by trehalose application. In co...

Peer Sikandar Shah1, Ghulam Nabi1, Maqsood Khan*1,2, Saddam Hussain1 and Jawad Ali Jan1

...arbohydrates metabolism, enzymes establishment and osmotic regulation This experiment was conducted at Agriculture Research Institute Tarnab, Peshawar during spring season (March-june) 2016. Randomized Complete Block Design was used with split plot arrangement having three replications. The sponge gourd Luffa acutangula cultivars (Jaipuri and Dharidhar) were allotted to the main plot while organic and inorganic regimes (i.e. Control, NPK (Nitrogen, Phosphorus...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
... degraded readily by gut-enzymes so that it doesn’t reach the circulation in biologically-active form so there seems to be less or no harm in consumption of milk from OT-treated-animals. But OT has remarkable effects on milk quality, composition and reproductive health of the dairy animals so it’s use in dairy industry should be discouraged.
...

Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat

...ases. Among fibrinolytic enzymes streptokinase (SK) is commonly used in the world to cure heart diseases because its low cost and efficacy. It’s a microbial origin enzyme produced especially by Streptococcus bacterial spp. Therefore, it is antigenic in nature due to microbial source which restricts its use. However, it is necessary to truncate antigenic regions present in the SK gene for the removal of streptokinase antigenicity. The basic goal of this r...

Muhammad Usman Ghazanfar1, Waqas Raza1*, Waqas Wakil2,3, Imtiaz Hussain4 and Misbah Iqbal Qamar

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

...very unique encoding the enzymes for replication. Our objective is to describe the relatedness of these HIV-1 pol genes with each other and its antiviral drug susceptibility which will provide more information on its sensitivity and resistivity to each antiviral drug. Seventeen partial genome sequences of HIV-1 Pol gene from some West African countries were retrieved from NCBI database. Evolutionary relationship was determined by Multiple sequence alignment us...

 K. Abbasi 1 , R. Wick2† and D. Zafari1

Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...solates, three chitinase enzymes were evaluated using three substrates. Based on the results of our chitinase assay, Beauveria bassiana, Lecanicillium muscarium, Paecilomyces sp. and Trichoderma atroviridae had the highest activity. We selected these four isolates to determine the optimum pH, temperature, and reaction time. Zymography was also used to demonstrate the chitinase activity of the four isolates. The biocontrol potential of 10 selected isolates was ...

Parsa Riaz and Muhammad Naeem*

Digestive Enzymes Activity with Gut Morphometric Parameter of Carnivorous Fish Wallago attu (Siluridae, Siluriformes)
...els of various digestive enzymes, such as lipases, proteases and amylases in the digestive tract of a wild, freshwater, carnivorous Wallago attu. Wallago attu is a fast growing catfish that belongs to family siluridae under the order siluriformes. It has good market demand as a food fish having high nutritional value, and high protein content in its flesh, Carnivorous in nature. Descriptive data of the studied traits included total body weight, total body leng...
Sumaira, Ali Mujtaba Shah*, Ghulam Asghar Solangi, Ifra Anwar, Qudratullah Kalwar 
...numerous fatty acids and enzymes. Hence camel milk has many beneficial effects, such as antiviral, antibacterial, anti-diabetic, anti-carcinogenic and anti-ageing. Besides, camel milk contains abundant proteins which are conductive to improve the immunity functions. Thus, it is necessary to illuminate the beneficial impact of camel milk and its composition. 
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
...tinine, bilirubin, liver enzymes, total, glucose and urea in blood significantly (P<0.05) increased. Fluctuations in mean erythrocyte counts (RBC), hemoglobin, PCV and total bilirubin were beyond the limits while others were within normal ranges in sheep infected with Clostridium perfringens type A. The total bilirubin, Liver enzymes, creatinine level, glucose and urea levels in blood were abnormal while others wer...

Muhammad Jan1*, Tanveer ul Haq2, Hina Sattar3, Madiha Butt4, Abdul Khaliq5, Muhammad Arif6 and Abdul Rauf

...tion the activity of all enzymes increased among all genotypes. The chick pea genotypes, GGP-1260, PGP-1426 and PB-1 were observed as drought-tolerant ones on the basis of biochemical, morphological and physiological parameters and these can be recommended for rain-fed areas crop selection. 

...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
...tricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding pattern identified two Fusarium species i.e. Fusarium oxysporum and Fusarium solani. In conclusion, PCR-ITS-RFLP proved to be an efficient and easy technique for the identification of wilt complex pathogens.

...
Yasir Sharif1, Saba Irshad1*, Ammara Muazzam1, Muhammad Hamza Tariq1, Ambreen Kanwal1, Sana Rasheed1, Mabel Baxter Dalrymple2 and Anam Tariq1
...ts’ CBCs and liver enzymes were: red blood cells 3.07x 1012±0.769x 1012/L, white blood cells 8.89x 109±2.849x 109/L, hemoglobin 8.01± 1.027 g/dL, platelets 321.68x 109±1.027x 109/L, serum ferritin 2773.3±1071.9ng/mL, alanine transaminase 117.12±32.001U/L, aspartate transaminase 84.77±18.223U/L and bilirubin 1.02±0.139mg/dL. CBC of con...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...iology such as digestive enzymes or structural proteins of the intestine are considered.

...

Md. Gulam Rabbany Rassel1, Kazi Afsana Homayra Orchy1, Md. Moshiur Rahman Khan1, Marzia Rahman2 and Md. Mahmudul Alam1*

Hematological and Serum Biochemical Indices in Calves with Navel Ill
...thy calves. Some leading enzymes such as ALT, AST, ALP, LDH, CK were elevated in affected calves than their healthy counterparts. Creatinine, BUN, LDL, HDL, cholesterol and triglyceride were also elevated remarkably. Mild alterations of electrolytes such as Na+, K+, Cl- and Ca+2 were also observed. The results demonstrated that navel ill may compromise vital organs which should be promptly treated once diagnosed. To prevent infection, navel area should be dipp...
Abdul Nabi Jatt1,2*
...extracellular hydrolytic enzymes and are considered as key components in global carbon cycle. The aim of this study was to screen marine snow associated bacterium, Citrobacter freundii B1 for the production of QS-signals particularly acyl-homoserine-lactones (AHLs) and their influence on the production of extracellular cellulase enzyme. Mainly, two different reporter ...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...ffect the level of liver enzymes. All the tested liver enzymes were observed to be present in the normal range in all the treated groups A, B, C and D. Feed conversion ratio was not affected in the treated groups and an average FCR of 1.6 was observed in all groups. From the present study it is concluded that indigenous S. cerevisiae can be isolated from different sources and can be used as a source of SCMP in broiler...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
... This bacterium produces enzymes known as extended spectrum β-lactamases which render broad spectrum cephalosporins and penicillins inactive. This study reports antibiotic susceptibility pattern, multiple antibiotic resistance (MAR) index and prevalence of extended spectrum β-lactamases among clinical isolates of P. aeruginosa collected from tertiary care hospitals of Peshawar, Pakistan.A total of 187 P. aeruginosa isolates were collect...
Ye Ge1, Chunmiao Zhao1, Weitian Xie2, Ying Liu2,* and Chunhou Xu1,*
...ing spores and digestive enzymes, we produced the protoplast as the parent strain of B. subtilis and Rhamnose lactobacillus and analyzed the production rate of spores, the ability to resist high temperature and hereditary stability. The results showed that RH-3 was a perfect protoplast from which 48 strains were obtained through regeneration, with a sporogenic rate of 69.1% and a colony number of 2.7×105 CFU/mL after an 80 °C...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...the level of antioxidant enzymes in the liver and pancreas tissue at dose dependent manner. Our finding clearly supported the beneficial effect of caffeic acid on not only the diabetes mellitus, but also alleviating Gestational diabetes mellitus.
...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
...ect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantification assays such as test tubes revealed significantly (p<0.001) strong biofilm after 5 days with significantly increased planktonic cells (after 3 days) and increased loosely bound cells (after 5 days). Similar...

Masood Sadiq Butt1, Sadia Aslam1,2, Rizwan Shukat1,*, Syed Qamar Abbas1, Muhammad Issa Khan1, Shadab Shaukat3 and Muhammad Shahid4

...able amount of proteins, enzymes and fats. It was considered as garbage of no financial value and disposed of without any attempt of recovery. The aim of this study was to optimize the hydrolysis conditions for production of protein hydrolysate with enhanced functionality. Minced rohu (Labeo rohita) waste mainly comprising of head, tail, fins and skin was considered as raw material to prepare protein hydrolysate using flavorzyme enzyme. The results show...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...healthy ones. Scavenging enzymes (SOD, CAT, POX and
PPO) were increased mostly in tolerant and resistant cultivars compared with susceptible
cultivars and healthy ones. The total number of phenol oxidizing isozymes (POX and PPO)
bands in infected plants of eleven cultivars was varied considerably by increasing, decreasing or
no changes comparing with healthy plants. The presence of Ryadg gene has been observed in...

Mohamed I. Azzam1, Safaa M. Ezzat1, K. A. El-Dougdoug2, Badawi A. Othman2

...
family. Restriction enzymes by EcoRI, HindIII and BamHI showed the presence of
double stranded (ds) DNA as well as heterogeneity among these phages. The results
showed that EcoRI produced 7, 6, 2, 9, 10, 8, 10, 7 unique fragments and HindIII
produced 2, 3, 0, 1, 2, 5, 4, 3 unique fragments while BamHI produced only 4, 0, 1, 1, 0,
4, 2, 1 unique fragments, for the eight phage isolates, respectively. Out of fi...

Aliya Munir1, Sajid Abdullah1*, Huma Naz2*, Khalid Abbas1, Tanveer Ahmed3 and Zahid Farooq2

...arkers like antioxidants enzymes have been successfully used in ecotoxicology field for evaluating the stress response of organisms exposed to pollutants. Therefore, this work was designed to evaluate the peroxidase (POx) level in kiney, gills and liver of fish Channa striata exposed to LC50 concentration (1.374µgL-1) of deltamethrin+endosulfan (DM+ES) mixture for 4-day. Sampling was performed after a 24-hr period. Fish were sacrificed and organs were co...

Dilan Ozmen Ozgun1, Gonca Alak2, Arzu Ucar2, Sinan Bilginer3, Halise Inci Gul3, Mahmut Kocaman2, Cem Yamalı3, Veysel Parlak2, Muhammed Atamanalp2, Ahmad Maslat4 and Telat Yanık2*

...cts of B4 on antioxidant enzymes of gill and liver, and changes in hematological parameters were determined in rainbow trout. For this purpose, rainbow trout alevins were exposed to 0, 0.25, 0.5, 1 and 2.5 mg/L of B4 and 1 ml of DMSO for 96 hours. Blood samples were collected for hematological analyzes and mortalities were recorded daily. Exposure to B4 caused significant changes in antioxidant enzymes of gill and liver of a...
Jia Li and Hong Yang*
...uantity, the activity of enzymes and functional of bacteria, were also increased due to the APG and alkaline treatments. Overall, the instantaneous enhancement of microbial activity and BaA bioavailability increased biodegradation efficiency.
...

Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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