Matheus Nunes Weber1, Simone Silveira1, André Felipe Streck1, Luís Gustavo Corbellini2, Cláudio Wageck Canal1*

Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf

E-mail | murtazhassan@yahoo.com

Bibi Tayyaba Batool1, Aemal Tareen1, Shahjahan Shabir Ahmed1, Hira Ejaz1, Mohammad Azam Kakar1, Syed Abdul Rehman2, Mohammed Saeed1, Zafar Ahmad3, Mohammad Masood Tariq3, Mohammad Arif Awan3 and Muhammad Naeem Shahwani1*

...ustify;">Brucellosis and bovine tuberculosis (TB) are a constraint to livestock production in Balochistan. The aim of this study was to determine the prevalence of brucellosis and bovine tuberculosis in cattle, goats and sheep in some primary dwelling districts of Balochistan. Our results showed that brucellosis was found 2.2% positive from Quetta district and 0.6% positive from Pishin district and b...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...sal patellar fixation in bovine.
...
Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2
...f commercially available bovine F. hepatica enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this test indicated significant (p<0.05) values for sh...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...Nucleotide sequencing of bovine osteopontin gene showed a novel DNA polymorphism 37421762 (C>A) in the 3’-untranslated region (3’-UTR) of the gene. Association test was performed to analyze its relation with milk composition but it was found non-significant in all analyzed traits. So, it was inferred that it might be due to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the ident...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...n levels were studied in bovine from three distinct temporal zones of Khyber Pakhtunkhwa province of Pakistan. Twelve hundreds ticks were collected from four hundreds animals comprising of two hundred and fifty cattle and one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamap...
Nosheen Rafiq1, Asmatullah Kakar2,*, Arshad Ghani3, Asim Iqbal2, Wali Mohammad Achakzai2, Shagufta Sadozai2, Mohammad Shafiq4 and Mohammad Alam Mengal5 
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...n mammals such as human, bovine, pig and rabbit, it also has major influence in the natural defense of the animal body. In this paper, by comparative study on BPI gene expression in various tissues between Rongchang pig and Landrace, we confirmed the expression of pig BPI gene in various tissues to determine the difference expression of antibacterial related gene among indigenous breed (Rongchang pig) and artificially cultivated breed (Landrace), The Rongchang...

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

...es used for diagnosis of bovine brucellosis on serological, bacteriological and molecular basis. A total of 141 cows from brucella infected farms under quarantine of the veterinary authorities were employed. Serological examination using BPAT, RBT and CFT in 141 cows revealed 109 (77. 3), 105 (74.47) and 104 (73.76) seropositive respectively. Relative sensitivity, relative specificity, positive predictive value and negative predictive value of BPAT, RBT and CF...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
.... coli isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...ion in the four domestic bovine species of Pakistan, which are normally less aggressive. Selected animal species were cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V158M is present in exon-8 of COMT gene. Each specie was subjected to blood sampling (n=20 each). DNA was extracted, purified, quantified and amplified through PCR by using specific primer set. Then Sanger&rsq...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...strength and mobility of bovine serum albumin (BSA) was different in all milk species.
...
Asad Ullah1, Umar Sadique Khattak2, Sultan Ayaz1, Muhammad Subhan Qureshi2, Imad Khan1, Ibad Ullah Jan2, Irfan Khattak1, Raheela Taj3, Sahar Nigar4,    Naimat Ullah Khan1,Mumtaz Ali Khan5 and Muhammad Luqman Sohail6,*
...sociated risk factors of bovine tuberculosis (bTB) among large ruminants of the five districts (Peshawar, Nowshera, Charsadda, Mardan and Swabi) located in the central zone of Khyber Pakhtunkhwa (KPK), Pakistan. A total of 2400 large ruminants were sampled by convenience sampling approach and tested by comparative cervical intradermal tuberculin test (CCIT) for detection of Mycobacterium bovis infection. Data regarding socio-demographic status, risk fac...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2

 

...R. Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more prone to disease. In the case of buffalo breeds, prevalence was more in Nili Ravi buffalo, i.e. 36.6% than Kundi breed, i.e. 33.3%. Prevalence of disease was found maximum in humid and plain area of Lahore, i.e. 56%. In dry, dusty and hot weather of...

Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1

Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...™) for controlling bovine mastitis through systemic dry period therapy,
present study was designed. A total of 20 dry pregnant cows were selected
randomly at the end of lactation and divided into two equal G1 and G2 groups.
Group G1 was treated with lincomycin@ 5mg/kg (IM) and spiramycin@ 10 mg/kg
(IM) (Inj. lispiracin®) at end of lactation and at 14th day pre calving while group G2
was kept as control. Samples of m...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...sporadically reported in bovines in Pakistan. During June 2014 to 2015, twenty five buffalos ranging in age between 7 to 9 years, were referred to surgery clinic at University of Veterinary and Animal Sciences, Lahore, Pakistan. The patients showed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure a...

Omer Baris Ince* 

...ically healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the disease-related status of the selected region, to investigate the active virus circulation and determine the antibodies formed against the nonstructural proteins (NSP...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ciola sp. infestated bovines (cattle and buffaloes) and their effect on various blood parameters (WBC, RBC, Hb, HCT and MCHC). For this purpose, 40 infected animals were selected. After dividing them randomly in four groups of 10 animals each, drug was administered to first three groups whereas last one was kept under positive control for comparison. Study revealed that Triclabendazole was most effective drug of all three in both cattle and buffaloes with ...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

...f the causative agent of bovine mastitis, Staphylococcus aureus. Ninety milk samples were collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (SFMT) was applied for screening subclinical mastitis ...
Muhammad Zain Saleem1,*, Raheela Akhtar1, Asim Aslam1, Muhammad Imran Rashid2, Zafar Iqbal Chaudhry1, Muhammad Adeel Manzoor1, Bilal Ahmed Shah3, Rais Ahmed4 and Muhammad Yasin5
... (causative agent of bovine) may cross the species barrier to infect the small ruminants which may complicate the control measures of brucellosis because most of the control programs rely on serological screening of brucellosis rather than molecular assay which could confirm the particular species circulating in ruminants. In this study 960 and 471 serum samples of goats and sheep were collected, respectively. After screening with Rose Bengal test (RBT), a...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

... dairy animals including bovines. However, only a little information is available about bacterial profile of the disease. A cross-sectional study was conducted to find the Etio-prevalence of bacterial species causing subclinical mastitis in a cohort of buffaloes at Khyber Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was ...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
 

Aftab Shaukat1,*, Khalid Medmood3, Irfan Shaukat2, Tauseef ur Rehman3, Muhammad Ahsan Naeem1,5, Ashar Mehfooz1, Muhammad Ijaz Saleem1, Zia-ud-Din Sindhu1, Shahid Ali Rajput4, Mubashar Hassan1,4, Saqib Umar1, Muhammad Ali Jamil1, Rao Zahid Abbas1 and Anas Sarwar Qureshi1

 

...ological changes (due to bovine anaplasmosis) were studied in Sahiwal cattle and crossbred cattle of the district Faisalabad of Punjab province, Pakistan. Therapeutic efficiency of Oxytetracycline-Imidocarb dipropionate combination was compared with Enrofloxacin-Imidocarb dipropionate against Anaplasma marginale (A. marginale). For this study, the blood samples of three hundred and sixty nine cattle were collected. Giemsa stain of blood smears sh...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...ffect of polymorphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinical (n=50) mastitis along with non-mastitic (n=50) cows from different dairy farms of Punjab. TNF-α gene was amplified with specific primer and sequenced to get the full-length seque...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
Ambreen Leghari1,2, Asghar Ali Kamboh2,*, Shakeel Ahmed Lakho1, Faiz Muhammad Khand1, Kanwar Kumar Malhi2,3, Iqra Bano Chandio4, Seema Baloch5 and Jan Mohammad Shah4
...ion on the prevalence of bovine tuberculosis (BTB) in randomly selected cattle (n= 160) was carried out in Hyderabad and Tando Allahyar districts. The animals were first screened through single intradermal tuberculin test (SITT) and both positive and negative reactors of SITT were further investigated through rapid test. Attempts were also made to isolate the Mycobacterium bovis organism from the milk and nasal secretions of cattle using the Lowenstein-...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...endogenous gene ABCA1 in bovine mammary epithelial cells (BMECs) to analyse the effect on intracellular triglyceride (TG) content by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, cell TG Assay and RT2 Profiler PCR array. The results showed that ABCA1 knockdown reduced the TG content in BMECs. On the other hand, the RT2 Profiler PCR array demonstrated that the expression levels of 12 genes, including fatty aci...
Memis Ozdemir
...on 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in Bos taurus cattle breeds, but have been not detected the RsaI restriction enzyme digestion site in ...

Ankur Abrol, Madhumeet Singh, Akshay Sharma* and Pravesh Kumar 

Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2,  Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1Aneela Zameer Durrani4 and Muhammad Usman4*

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

...tigate the prevalence of bovine tuberculosis (bTB); detection of Mycobacterium bovis (M. bovis) infection in lactating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevale...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...quality of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL bus...

Md. Zubael Islam, Rukhsana Amin Runa and Md. Mahmudul Alam* 

...otential risk factors of bovine foot diseases in certain areas of Sirajgang district. It is recommended that further robust studies are required to address the foot disease burden in lactating cows. 

...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...onotic Moroccan goat and bovine strains. The amino acid sequences of the antigenic regions inside VP8* proteins showed high conservation between the amino acid sequences of human and animal strains bearing P[14] proteins, and revealed several changes with respect to the RotaTeq™ and Rotarix™ vaccine strains. This report suggests a possible reassortment event between human and domestic animal rotaviruses. Continuous surveillance in the post vaccine ...

Rafiullah Khan1, Waseem ur Rahman1, Misbah Ullah2, Kamran Afaq3, Muhammad Amjad1, Sakhi jan1 

AGE EFFECT ON THE MECHANICAL PROPERTIES OF HIP JOINT BONE: AN EXPERIMENTAL INVESTIGATION
...t bone specimens of male bovine of different age groups were tested for fracture toughness, young modulus
and tensile strength in the longitudinal and transverse directions of the collagen’s fibers. Compact tension specimen
was used for the fracture toughness, while for young modulus and tensile strength, rectangular flat specimens were
used. The test procedures were similar to ASTM (American standard for testing and measurements) te...
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
...k. In the present study, bovine skeletal muscle satellite cells (bSMSCs) were cultured and the Wnt/β-catenin signaling pathway was activated by LiCl. Investigation of the patterns of differentially-expressed miRNAs and analysis of bSMSCs target genes were then carried out using microarray and bioinformatics. The data revealed significant changes (>2-fold) in 29 out of 783 differentially expressed miRNAs, 12 of which were up-regulated and 17 were down-r...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
.../i> muscle (LM) of fetal bovine and induced adipogenesis. To optimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated c...

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
...t, caprine, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After reviewing 82 published articles, a total of 48 studies from 29 articles were eligible to be included in this meta-analysis study. The total seroprevalence of bluetongue in apparently healthy sheep, goat, cow and camel at animal level based on ELISA test was 50.4% (95% CI= 43.5–57.2), 79.2% (95% CI= 70.7–85.8), 3.3% ...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...viability of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult bovine serum (ABS) to be evaluated as alternative to FBS. ATFs were cultured in DMEM-HG and supplemented with 10% FBS, 10% AES and 10% ABS. At passage 4, each group showed same morphology and appearance, there w...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
...is the cause of enzootic bovine leukosis and has worldwide distribution. This is study reports presence and genetic diversity of BLV in cattle of two districts of Punjab, Pakistan. Blood samples (n=400) taken from local (n=200) and exotic (n=200) breeds of cattle were processed for complete blood count. Based on lymphocyte counts, samples were divided into two groups (A, lymphocytosis, B, normal lymphocytes) and tested through ELISA and nested PCR for serologi...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

...on of betacoronavirus 1 (bovine coronavirus) BCoV in newborn gastroenteritis calves in the Delta Region, Menofia Province, Egypt, also for isolation and identification of the local circulating BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections which leads to serious complication which may be fatal in young calves up to 3 months ...

Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*

...les on the prevalence of bovine surgical affections in Mymensingh and its surrounding localities.

...

Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*

...fy;">Buffaloes and other bovines suffer from a fatal disease, “Hemorrhagic septicemia” (HS), which is produced by a gram-negative bacterium “Pasteurella multocida.” Existing literature has established bacteria’s pathogenesis, but still, the literature about the role of bacteria and its immunogens on the demonstration of clinical signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...ork, the sequence of the bovine STAT5A gene was analyzed to identify single nucleotide polymorphisms and its effect on evolutionary divergence. Nine polymorphisms, six in intronic and three in exonic regions, were identified in STAT5A gene. One exonic polymorphism G→A, in exon 5, was significant that causes a non-synonymous amino acid change from Serine (S) to Asparagine (N). Further, 64 genetic variants were also identified in STAT5A...
Yawang Sun, Yongjiang Wu, Jingbo Chen, Zili Wang, Juncai Chen, You Yang and Guozhong Dong*
...opolysaccharide (LPS) in bovine mammary epithelial cell line MAC-T cells. Cells were cultured for 48 h with different LPS levels (0, 0.1, 0.5, 2.5, 12.5, and 100 ng/mL). The results showed that cell viability was negatively correlated with LPS concentrations. The production of reactive oxygen species increased in a dose-depend manner. Cellular concentrations of oxidative damage markers were positively correlated with applied LPS concentrations. Total antioxida...
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...oots was investigated on bovine herpes-1 (BHV-1) infection in vitro. An ethanolic
extract of Salvadora persica was used in two concentrations. MDBK cells grown on
Minimum Essential Medium (MEM) were used for virus propagation and titration. The
effects of two concentrations of Salvadora persica on viral growth in MDBK cells as
well as cytolytic activity of BHV-1 were evaluated at different times pretreatment
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...logous with the vaccinal bovine strain.

...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

... designed to investigate bovine rotavirus antigen in fecal samples of forty diarrheic new borne calves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotaviru...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...9-11 day and Madin Darby bovine kidney (MDBK) cell culture. Identification of virus by indirect immuno fluorescent antibody technique (IFA) which was considered a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by...

El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.

... report the isolation of bovine ephemeral fever virus (BEFV) field strains from clinically infected cattle and buffaloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutralization and i...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

...genome. Infection by the bovine ephemeral fever virus (BEFV) was noticed in Dakahlyia governorates during the summer season of 2015. Twelve samples of buffy coats were collected from dairy farms suspected from clinical investigations to be infected by bovine ephemeral fever virus. The virus was isolated intracerebrally in suckling mice then successfully identified by indirect immunofluorescence technique in the brain section...

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and M. A. Shalaby2

...y tests for detection of bovine viral diarrhea virus as possible extraneous virus contaminant using RT-PCR and detection of mycoplasma as possible bacterial contaminant using PCR technique. All of the four batches were positive when tested using specific primers and they were free from BVDV or mycoplasma contamination. Results in this study showed that molecular techniques could be used for rapid evaluation of PPR vaccine including RT-PCR for identity testing ...
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...iv>
on Madin-Derby bovine kidney cell line ( MDBK ); confirmation of LSD isolated virus was done by
using Electron microscopy (EM) on suspension of cell culture passage of the virus, Serological
identification was done by serum neutralization test ( SNT ) using reference antisera which confirm
presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR)
gene segment of th...

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*

Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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