Tom Gilson & Carson Weitnauer (eds), True Reason: Confronting the Irrationality of the New Atheism (Grand Rapids, MI: Kregel Publications, 2013), 315pp, US$17.99 (paperback), ISBN 978-0-8254-4338-1
Reviewed by Gregory W. Dawes, Associate Professor of Philosophy
Science, Religion and Culture
...s posed by biblical endorsements of slavery and genocide. No review could do justice to so wide a range of issues, so my aim here is simply to offer some general reflections on the strategies employed....
Muhammad Zubair, Sajid Mahmood Sajid
... terms of improvement of semen....
Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
... quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen
YaQiu Liu and ZhiJian Wang*
... μm) and the basement membrance (0.27±0.05 μm) between them is similar to that of the respiratory organs in other vertebrates.Intestinal tract has digestion and respiration functions. Anterior intestine performs digestion/absorption. Posterior intestine performs gas exchange/accessory respiration. In middle intestine, digestion and respiration overlap.
...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...n the cryopreserved bull semen. Experiments were carried out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 a...
Ayşe Gül Harlıoğlu*
...ns of unfertilized eggs, semen, fertilized eggs, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the unfertilized eggs. On the other hand, PUFA was the highest followed by SFA and MUFA in the semen. The proportions of th...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...g standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60oC, Protocol III (concentration of dye in staining solution); slides were stained with 1, 5, 200...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...n-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straw...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...erienced in bulls during semen collection by electro ejaculation method. The normal electro ejaculation method of semen collection (Method I) was compared to a modified method involving three stages of graduated electrical stimulation (Method II) in four crossbred bulls. The results showed that intensive muscle spasm, bull struggling and arc back were reduced (p ˂ 0.05), as well as the time of penile protrusion (p = 0.0...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...Friesian) to compare the semen quality. Semen samples were individually diluted with egg yolk extender and tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed semen. Data were subjected to one way analysis of variance. High sperm individual motility (85.12 ± 0.51%), pro...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...from cows inseminated by semen of Black-and-White Holstein-Friesian bulls and giving birth to single calf. The following information was taken from each animal: age at first insemination, age at first calving, number of semen portions attributable to successful insemination, length of pregnancy and periods of: insemination service, postpartum resting period, interpregnancy period and intercalving period. The study also deter...
Effect of Post-coital intervals on DNA STR profiling of semen donor in the post-coital vaginal swabs
Manzoor Hussaina,b
...rate the DNA profile the semen donor in the post-coital vaginal swabs. Sixty two post coital vaginal swabs (x3) were collected from six volunteers at different TSI (time since intercourse). Out of these, 42 post coital vaginal swabs (up to TSI 5 days) were used to generate the autosomal DNA profile of the semen donor and twenty of the post coital vaginal swabs obtained at the TSI of 6, 7, 8, 9 and 10 days were not tested for...
Irfan1,*, Arshad Javid7, Muhammad Ashraf2, Athar Mahmud3, Muhammad Altaf4, Syed Makhdoom Hussain5, Muhammad Shahbaz4 and Khalid Javed Iqbal6
...>Meleagris gallopavo semen were recorded and hatchability percentages of the artificially inseminated hens were assessed. To study the effect of natural mating on hatchability 15 (3♂, 12♀) M. gallopavo were randomly selected, were kept separately and were allowed to mate through stud mating. For artificial insemination, male (n = 3) and female (n = 12) birds were kept separately. Semen of the male bird...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
...measured weekly, whereas semen quality, and histopathological profile were investigated at week 10.The obtained results reveal that under a long photoperiodthe sexual activity of the control lasted only 04 weeks, characterized by significant increase in the testicular volume, followed by spontaneous gonadal regressionup to week 10. Consequently, an azospermia and lack of germ cells in control birds was noticed,which confirm the testicular regression.In the tre...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...selenium addition to the semen extender on chilled and frozen thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ra...
M. Tariq Javed1, Nazir Ahmad2, Gulbeen Saleem3, Haleema Sadia4, Farkhanda Manzoor5 and Ghulam Hussain Dilbar
EFFECT OF AGE AND SEASON ON SEMEN CHARACTERISTICS OF BUFFALO BULL - A REVIEW
...re on various aspects of semen parameters in buffalo bull at different age and in different seasons has been published with conflicting reports. An effort is made to review the literature gathered from different sources on semen characteristics in buffalo bulls which will be helpful to researchers and allied professionals in future research or policy planning. The review is bifurcated in different sections for easy understan...
Zhongquan Zhao*, Tianyuan Yang, Lei Qiao, Qijie He and Zinuo Dai
...s does in the breed. The semen quality was high in different seasons. Dazu black goats might have shorter gestation period (147.9±1.7 days) and have longer kidding interval (237.8±4.6 days). The percentages of singletons, twins, triplets and quadruplets born were 1.98, 47.27, 40.02 and 10.29 %, respectively. The mean litter sizes (2.60±0.72) were the highest among the reported meat goat breeds in China. Dazu black goat reached the highest ...
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
...d supplementation on the semen quality of breeding bulls. A total of 8 adult (≥2 year) bulls (2 each from Jersey, Friesian, Sahiwal and cross bred) were selected with body condition score (BSC) at a scale of (0-5). The results showed that mean semen volume was significantly (P<0.05) high in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey bull while live sperm percentage was...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...rent antioxidants on the semen cryopreservation in Guangzhong donkey by testing indices of sperm motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen w...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...ductive performance, and semen quality of Friesian Sahiwal crossbred bulls during summer season. A total of 24 Friesian Sahiwal crossbred bulls at nearly similar age and body weight were selected and allotted erratically into 3 treatment groups, denoted as A, B and C for a period of 2 months (May and June). Each group was assigned 8 bulls. Bulls in group A were not fed with any dietary fat (control group) while bulls in group B and C were offered 100 and 200 g...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...ect post-thaw quality of semen. In the current study, we endeavored to ascertain the most favorable application of L-cysteine for cryopreservation of Achai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental materia...
Sameh S. Tawfik, Ahmed A. Elkady* and Ehab T. Mohamed
...;s space and thickened basement membrane, while in TSE-treated and irradiated-group, renal-tissues showed minimum-damage with or without few degenerative changes. TSE acts as a potent antioxidant and anti-inflammatory drug to prevent and/ or ameliorates the hazardous-effects of γ-rays
...
Yanyan Yu1,2,3, Yizhong Hu1, Qipeng Zhang1, Rongquan Zheng1,4,*, Bing Shen1, Shenshen Kong1 and Ke Li1
...al.
...
Yun-Feng Liu, Yong-Mei Guan, Dan Chen, Jia-Le Chen, Chen Jin, Wei-Feng Zhu, Lu Wu and Li-Hua Chen*
Amit Sharma1* and Pankaj Sood2
...tors affect affects the semen cryopreservation viz. species, breed, semen collection technique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...ng the quality of frozen semen due to the use of GnRH analogue additive (buserelin acetate) for bovine semen and its low cost of use in routine semen production will allow manufacturers of insemination doses to improve the quality of their product.
...
Paul Oludare Adetunji* and Safiriyu Idowu Ola
...from 16-22 weeks of age, semen quality parameters (semen volume, semen concentration, sperm output, sperm motility and sperm abnormalities) from 26-52 weeks of age and sexual ornaments’ morphometry (Comb Length-CL, Comb Height-CH, Wattle Length-WL, Wattle Height-WH and Inter Pubic Distance-IPD) from 16-24 weeks of age. Data on onset of puberty and semen
Muhammad Qasim Khan1*, Syed Athar Masood1 and Shahzad Naeem Qureshi2
REFORMULATION OF PRODUCTIVITY IMPROVEMENT MODEL FOR PUBLIC SECTOR AUTOMOBILE REBUILD ORGANIZATIONS
...ite huge budgetary disbursements. On the contrary, private sector organizations achieve high productivity and output as these organizations work to maximize their profit. This paper attempts to identify reasons for low productivity and reduced output of public sector automobile rebuild organizations of Pakistan. Input in this research was sought primarily from top, middle and lower management of public sector automobile rebuilt organizations. Data from a ...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...f Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender suppl...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...ion of arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion p...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
... h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. S...
Qianbao Wang, Zhengyang Huang, Shoufeng Li, Huayun Huang, Chunmiao Li and Zhenhua Zhao*
...ody weight and improving semen quantity and quality in broiler breeder males. A total of 60 F line broiler breeder males from 20 maternal families were averagely distributed into three groups received treatments of 135.5, 125.5 and 115.5 g/d diet respectively with ME 2770 kcal/kg and CP 15.4% from 23 wk of age. Fertility trials were conducted in another three groups of F line hens with 20 hens in each group. A significantly positive interaction between dietary...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...m cells located on the basement of the seminiferous tubules of the testes, are the only kind of adult stem cells that can pass their genetic material to offspring and are also capable of proliferation and differentiation, ensuring the efficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary...
Sohail Akbar1*, Muhammad Shafiq2, Muhammad Yaqoob2, Muhammad Farooq Iqbal2, Kashif Ishaq2, Muhammad Kamran2, Shazia Shamas3, Arbab Sikander4 and Muhammad Hashim5
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
...years old) were used for semen collection, and the ejaculates were pooled and divided into five equal aliquots. The samples were diluted with a solution having different additives at 37 °C. The first two groups contained L-Cystine as an additive (2 and 4 mM), the next two groups had methionine as an additive (2 and 4 mM), and the last group had no additives (control). After dilution, the semen samples were cooled at 5 &d...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...tamin E) were added into semen diluent and assessed the freeze-thawed semen quality parameters. The ejaculates were collected from two crossbred groups (Sahiwal × Holstein-Friesian), and (Achai × Jersey) and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concentrations (1.2, 2.4 and 4.8 mmol) of vita...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...e OS level in the frozen semen. This study was to determine the addition of different concentrations of antioxidants to evaluate oxidative stress and fertility of frozen semen in horses. Seven stallion were used for semen collection in this study. A total of twenty ejaculates was collected from three healthy stallions twice a week for the experiments 1 and 2. All ejaculates were divided in...
Ahmed F. Afify1, Mohamed A. Shalaby2, Ahmed A. El-Sanousi2 and Amal S. Gaber1
...Ablood
and 4 semen samples. Serologically, indirect ELISA was performed on 540
serum samples. 130 samples were clearly strong positive at a percentage of 24%.
Molecular detection of EAV genome was performed only on 8 selected semen and
EDTA-blood samples which were giving highly distinct positive reaction in the
indirect ELISA. All samples were found negative to the...
Aamer Abbas1, Jabbar Khan1*, Mir Abid Hassan2, Asif Qayyum3 and Hamed Shafiq4
...togenesis and quality of semen. Moreover, metals can lead to decreased or no production of prostaglandin, seminal fluid and endocrinology of reproductive process and thus results in male infertility. It was also here attempted to characterize the drastic effects of heavy metals on male infertility, the hormone analysis and genetic determination of SRY gene as some of infertile human had smaller sized testis. Blood and semen
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
Nacira Djabali1*, Souad Dougat2, Wahida Haddadi2 and Lamis Fadhli2
...he gonadotropic axis and semen biology in the rabbit, Oryctolagus cuniculus. Thirty adult male rabbits were divided into three groups (n = 10): a control group and 2 groups treated with EGME. The solvent was applied at two increasing doses: 50 ppm and 150 ppm for five successive weeks, orally. The level of the circulating gonadotropin hormones luteinizing hormone (LH) and follicle stimulating hormone (FSH), the level of testosterone, the biological char...
Tabinda Urooj1,*, Bushra Wasim1, Shamim Mushtaq2, Syed Nudrat Nawaid Shah1, Lubna Faisal3, Moazzam Ali2, Nabeela Rasheed1 and Syed Faizan Ali Rizvi4
Mohammed Sirajul Islam1,2*, Nurhusien Yimer1*, Wahid Haron1 , Faez Firdaus Abdullah Jesse1, Mark Hiew Wen Han1, Mamat-Hamidi Kamalludin3, Wan-Nor Fitri1, Ubedullah Kaka4, Abdul Quddus1,5
...etermined by the time of semen ejaculation and quality. The results revealed significantly (p<0.05) greater body weight in KK × Brangus bulls (230.50±9.3 kg) as compared to the pure-bred KK bulls (204.5 ±13.2 kg) at 24 months of ages. KK bulls attained sexual maturity at an earlier age of 21.5±0.4 months, while KK × Brangus bulls attained at 28.8±0.5 months. The g...
Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra
...animal history, means of semen supply or the methods used for its introduction can give clues about these findings....
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...usion of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control where no Gal...
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
...24-25 months of age) and semen samples from bulls. Semen was collected once a week from all bulls which were divided according to semen quality to high and low fertile bulls (5 for each). Reaction time and semen characteristics were determined. Blood from bulls and calves, and seminal plasma of bulls were separated by centrifugation, and protein analysis...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...ed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0.10 versus 3.61 ± 0.11, P <0.001; total sperm (× 109): 2.56 ± 0.06 versus 5.12 ± 0.09, P <0.001; motility (1–10) 7.42 ± 0.55 versus 9.08 ± 0.57, P <0.05; relative survival: 35.85 ± 2.94 h versus 67.4 ± 2.77 h, P <0.001). The results...
Tatyana Mamontova1*, Konstantin Katkov1, Natalia Kizilova2 and Ali-Magomet Aybazov1
...ed in the autumn period (semen volume: 0.96 ± 0.14 ml versus 1.42 ± 0.15 ml, P <0.05; sperm concentration (× 109 ml-1 ): 2.66 ± 0.10 versus 3.61 ± 0.11, P <0.001; total sperm (× 109): 2.56 ± 0.06 versus 5.12 ± 0.09, P <0.001; motility (1–10) 7.42 ± 0.55 versus 9.08 ± 0.57, P <0.05; relative survival: 35.85 ± 2.94 h versus 67.4 ± 2.77 h, P <0.001). The results...
Luke Chukwudi Ali*, Nnanna Ephraim Ikeh, Bright Chigozie Amaefule, Amarachi Linda Obinna, Ndubuisi Samuel Machebe
..., respectively. Prior to semen collection, rabbit bucks were trained three times per week for semen collection with an artificial vagina (AV). At the end of the 10 weeks, semen collection and evaluation were carried out. Thirty six (36) rabbit bucks were slaughtered and testes collected and dissected for testicular morphometry and histomorphology evaluation. Semen<...
Gamal Shawki1, Tawfek Mohamed Barakat1, Attia Mohamed Samy2, Ahmed Alhamed AL-Mejren2, Ayman Mesalam1*
...ry, sexual behaviors and semen parameters of purebred Arabian stallions in Kuwait. Nine fertile stallions aged 4–22 years were used for assessment of testicle biometry, sexual behaviors and semen quality collected using artificial vagina throughout the year. Our results showed a clear monthly differences with higher semen quality (P < 0.05) especially in s...
Abd El-Moniem Ali S. Mahgoub, Ahmed Mohamed Abd El-Hafeez, Mahmoud Yassin Mohamed*, Al-Moataz Bellah Mahfouz Shaarawy, Mohamed Ibrahim Nassar
... growth performance, and semen quality. Animals were randomly divided into four groups and fed four different rations. Ration 1 (R1) was the control (60% concentrate feed mixture + 40% clover hay). The remaining rations were supplemented with sesban hay by 10% (R2), 20% (R3), and 30% (R4) as a replacement from the clover hay ratio in the control ration. Results showed that supplementation of sesban hay increased the nutritive value of digestible crude protein ...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM CYS, 1mM GLU + 5mM CYS, 1mM GLU + 10mM CYS and Control). ...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...ods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63±16.54% and 52.76± 8.88%, and 53...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
...n artificial vagina. The semen was proceeded until the frozen semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by using CASA with the Sperms Vision Version 3.7.5 program. The results in this study showed that sperms motility in Bali-horned bull was significant...
Idrissa Sacko1, Madou Dao1, Souleymane Sanogo1,2*, B. Michel Orounladji2, Kaly Diakité1, Diakaridia Traore3, Mamadou D. Coulibaly1, Amadou B. Cisse1
...n crossbred goat’s semen in Mali. From January to August 2020, a total of 45 ejaculates have been collected from 03 bucks (5/8 Anglo-Nubian, 3/8 Sahelian Goat) using artificial vagina. Semen volume, mass motility and live and dead sperm counts (spz) were measured by direct reading and electronic microscopy with a direct image transfer system on screen, respectively. Sperm concentration and total sperm count in ejaculat...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...s is expected to improve semen quality. This study aims to determine the effect of giving the hormone GnRH in various doses on the quality of semen in Ongole Crossbreed (OC) bulls. The study used 18 OC bulls with three GnRH treatments, A (one dose/5ml/animal), B (half-dose/2.5ml/animal), and C without GnRH. The GnRH was administered once by intramuscular (IM) injection. Semen collection wa...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
..., 1.5, and 2.0 % for ram semen extender during freezing steps. Semen was collected by an artificial vagina from five adult Rahmani rams twice-weekly for five weeks. Semen was pooled, split into five replicates, and prepared to be frozen. In 1st replicate, semen was extended in Tris egg yolk (TEY) is contained synthetic sources of energy (fructose), antib...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a signi...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyze...
Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4
...ondition score, the best semen quality, general health, and an efficient estrus detection technique....
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...tics of fresh and frozen semen quality of Aceh cattle at different ages. Three Aceh bulls were involved in this study. The semen was collected from each bull aged 6 to 10 years and the fresh and frozen semen quality was evaluated directly. The results showed that the bull age significantly affects semen volume, pH, sperm concentration, total sperm, total...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
... spermatozoa. Qualifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled ...
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...quality fresh and frozen semen of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...ryopreserved rabbit buck semen under HS conditions. A total of 40 APRI line rabbit bucks (7months old and 3.00±0.20 kg live body weight) were divided into four treatments (10/treatment). The 1st, 2nd, 3rd, and 4th treatments were orally administrated with 2 ml distilled water with 0,100, 200 and 300 mg of PEE/buck, respectively, for 40 days, as a treatment period, and then semen was collected twice/week for 12 weeks. ...
Ibrahim Samir Abd El-Hamid1, Alaa Emara Rabee2, Moustafa Mohamed M. A. Ghandour2, Rasha Salah Mohammed3, Ahmed Mohamed. Sallam4
...hytochemicals mixture on semen quality, blood metabolism, oxidative status and testicular structures. Three groups of fourteen Damascus bucks were formed. The first group (control, n=4) served as the control group. The second group (n=5) supplemented with 10 g/head/day of plant herb mixtures (PHM), along with turmeric (Curcuma longa), ginger (Zingiber officinaleor), and garlic (Allium sativum). The third group (n=5) supplemented with 5 g/head/day of commercial...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2
...tty acid compositions of semen seminal plasma in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) were analyzed in fresh semen by gas chromatography (GC) and investigated the relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus myk...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
... was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were sequentially incubated for 15 minutes at 37 ºC and cooled at 5 ºC for 2 hour than packed in 0.25...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
... oxidative stress during semen preservation can cause a decrease in motility, viability, and damage to plasma membranes that can reduce fertility rates. The use of antioxidants in semen preservation is very important to suppress lipid peroxidation so it can maintain the quality of the sperm. This study aimed to determine additional honey to the quality of KUB rooster sperm during cold storage. The se...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
... physiology and site for semen deposition or method of embryo transfer. Therefore, this review was conducted to collect and consolidate the current knowledge of assisted reproductive techniques of males and females in addition to their importance for oocyte maturation, artificial oocyte reprogramming, embryo development and their resulting newborns.
Keywords | In vitro, Oocyte, Embryo production, Embryo transfer, Micromanipulatio...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
...dy aimed to evaluate the semen quality and the proportion of spermatozoa X and Y from sexed semen using the Percoll Density Gradient Centrifugation method. The quality observed included individual motility, viability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexed semen
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...ity and proteomic plasma semen as fertility performance in Bali-polled bull. The utilization of Bali-horned bulls as a point of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm mo...
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
...uality of Pesisir cattle semen stored at 5oC. The study was conducted on freshly collected Pesisir cattle semen diluted in egg yolk citrate with or without tomato juice viz., P0 (100% egg yolk citrate), P1 (90% egg yolk citrate + 10% tomato juice) and P2 (80% egg yolk citrate + 20% tomato juice). After diluting the semen samples were stored at 5oC for 4 hours. The observed variables includ...
Doaa H. Assar1, Rasha A. Al-Wakeel2, Zizy I. Elbialy3, Mahmoud M. El-Maghraby4, Helmy K. Zaghlool5, Adel A. El-Badawy4, Abdel-Khalek E. Abdel-Khalek6*
... antioxidant status, and semen quality of cross-bred lambs (½ Finnish × ½ Ossime). Twenty-four lambs aged 10 months with 32.93±0.48 kg LBW were distributed into three groups (8/group). Lambs in G1 (controls) were fed a basal diet without treatment. In G2 and G3, the daily diet was supplied with 1 and 5 g SPL /kg from 10 to 15 months of age. Dietary SPL supplementation significantly increased body weight, count of red and white blood ...
I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*
... aimed to provide frozen semen of Kintamani dogs for rapid improvement of the quality and population of Kintamani dogs with the specific target of evaluating the fertilization capacity of frozen semen of Kintamani dogs using the artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. Bitches...
Hasanain Jihad Neamah
...ear extract (PPE) in ram semen on sperm parameters during cooling storage. Three levels of PPE were added to the diluted ram semen (PPE1=5mg +Tris extender, PPE2=10 mg +Tris extender, and PPE3=30 mg +Tris extender), and compared with control group (Tris extender without addition of PPE). Motility of sperm, hypo osmotic swelling test (HOS-Test), and dead sperm percentage were assessed after 2 and 48 hour at preservation. The ...
Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago
...characteristics of fresh semen of the Kokok Balenggek chicken (KBC) as well as fertility, fertile period, and sex ratio as a result of artificial insemination with variations in spermatozoa concentrations. Semen from 12 KBC was collected and evaluated to determine their characteristics (motility, viability, concentration, and abnormalities). Semen showing motility >70% was diluted into ...
Lalu Ahmad Zaenuri*, Rodiah Rodiah, Adji Santoso Dradjat, Oscar Yanuarianto, I Wayan Lanus Sumadiasa , Lukman Hy
...n in a basal diet on the semen quality of the Kacang Buck. This study was conducted on 10 mature bucks with good libido and an average age and body weight of 2.7±0.35 years and 28.4±2.4 kg, respectively. All experimental bucks received a daily diet of native grass equal to 10% of body weight from day 1 to day 20. From days 21 to 35, they received a diet the same as days 1 to 20, in addition to SGtab. Semen was ...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
Achmad Firman1*, Sondi Kuswaryan1, Lilis Nurlina1, Muhamad Hasan Hadiana1, Marina Sulistyati1, Unang Yunasaf1, Dwi Cipto Budinuryanto2, Iman Trisman3
... as the number of frozen semen distribution, the number of cow acceptors, the number of cow pregnancies, the service per conception rate, the number of calf’s birth, and the economic impact. Data collected by Directorate General of Livestock and Health (DGLH) was for five years. The Key Performance Indicators utilized to analyze the program performance. The incremental capital-output ratio (ICOR) model was used to determine the economic valuation. The re...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
... able to maintain better semen quality. ...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...e adding antioxidants to semen extender on canine sperm during 12 days of chilling storage. Twenty ejaculates from five American Bully dogs were used. The sperm quality was determined by system automatic sperm analyser for sperm motility parameters, and by confocal laser scanning microscope for sperm membrane integrity parameters. Sperm lipid peroxidation was also analysed through malondialdehyde production. The study found that there was a gradual decrease in...
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
...q. Bovine sex-controlled semen for artificial insemination were used to obtain different stage embryos: bovine 8 cell XX embryo, 8 cell XY embryo, 16 cell XX embryo, 16 cell XY embryo, morula XX embryo, morula XY embryo, blastocyst XX embryo, and blastocyst XY embryo. A sequencing library was constructed by the Smart-Seq2 amplification. The transcriptome was sequenced by Illumina HiSeqXten high-throughput sequence technology, and effective sequences were analy...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
...under 100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the sperm. The optimal dosage for maintaining the integrity of the dog sperm’s membrane and enhancing its property parameters was determined to be a supplementation of 100 µg/ml essential oils, which demonstrated a significant difference from the control...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...polis supplementation on semen extender of Egyptian buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5–5 years. The collected
Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4*
...tified with conventional semen quality assessments, but relative in vivo fertility varies significantly for camels considered fertile. Aim; Study the blood and seminal plasma aldosterone and testosterone levels of male dromedary camels and their outcome on semen parameters and fertility. Methods; From breeding history records, depending on fertility status, sixty camels had divided into two groups; control “fertile&rdq...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...fore fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen with -20 oC and growth factor concentrations were measured using the ELISA met...
Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1
...amination, vas different semen analysis and plasma FT4 level were measured at week 10. Results showed that under long photoperiod, the sexual activity of the control group lasted for 06 weeks, characterized by a significant growth in the testicular volume, followed by spontaneous gonadal regression up to week 10. Azoospermia in control birds was noticed, with a significant increase in FT4 level and a fall of flying feathers in the 8th and 10th weeks, which ass...
Muhammad Haseeb Malik2, Muhammad Moaeen-ud-Din1*, Ghazal Kaukab Raja2 and Feroza Hamid Wattoo2
...ublic as well as private semen production units. The present study was designed to develop standard molecular markers for Sahiwal to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for each Sahiwal and Crossbred cattle respectively. Candidate molecular markers present in Sahiwal but absent in Crossbred and vice versa were detected using amplified fragment length polymorphism method. Eleven markers were develope...
Wisit Ketpanyapong1, Kulisara Marupanthorn2*
...mpair boar fertility and semen quality. We investigated the impact of Moringa oleifera leaf extract (MOLE) supplementation on blood parameters, serum antioxidant status, and sperm quality in 24 Duroc boars at 1.0-1.5 years. The boars were randomly divided into four groups of three replicates each and their feed was supplemented with MOLE at concentrations of 0, 10, 50, and 250 mg/kg body weight (BW). Supplementing Duroc boras with MOLE increased leukocyte, ery...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...n chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4th day of observation showed that the addition of ascorbic acid as NEZ at a dose o...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
...is-buffer diluent on the semen quality of Kacang goats at 5°C storage. Semen samples were collected twice a week from a 2-year-old Kacang goat using an artificial vagina. The study design consisted of three treatments; T0, T1, T2 and T3, consisting of 0%, 10%, 15%, and 20 % of sugarcane juice, respectively. The measured parameters included progressive motility, viability, and abnormalities of spermatozoa. The data obtain...
Yi Zhao1,2,3, Lei Luo1,2, Liqi Tan4, Jianhua Huang1,2, Dongliang Liu1,2,5, Shigui Jiang1,2 and Lishi Yang1,2*
...partial rupture of the basement membrane (BM) in hepatopancreas, as well as the deformation of epithelial cells and respiratory epithelial cells of BM in gill tissues. While the temperature stress (30°C and 10°C treatment) caused the deformation of epithelial cells and respiratory epithelial cells, making rupture of the BM, comparing with the group at 25°C. The study provides the data about the response of crayfish to temperature stress at the mole...
Mohamedl , Shimaa D.; Ghareeb2, A.; El-Dougdoug , K. A. and Bekheitl , H.K.
...ritrophic membrane and basement membrane and red colour appeared due to presence of granules particles. The morphological structures of P. operculella infected larvae with granulosis virus as well as healthy ones were examined by SEM. It showed complete destruction of external larval body , abdominal segments overlapped in some , prolegs and thoracic legs reduced in size and also the circular shaped spiracles converted to oval shape with rupture and disrupted ...
El Sayed l , Yasmein A.; Ghareeb2 , A.; El-Dougdoug3, K. A. and Bekheetl , H. K.
...ritrophie membrane and basement membrane and scatter of viral polyhedra and small empty vacuoles were observed....
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...st-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in this investigation. Semen collection was conducted weekly a 12- week period using an artificial vagina. Lycopene was added to the skim milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly collected sem...
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
...conducted five times for semen collection and using treatments; T1 = Medium concentration of 10% and 30%, T2 = Medium concentration of 15% and 45%, T3 = Medium concentration of 20% and 60%. Parameters measured in this study were motility, viability, abnormality, membrane integrity, acrosome integrity, movement pattern and proportion of spermatozoa. The data obtained were analysed using one-way analysis of variance (ANOVA). A Descriptive Statistical test used t...
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
... collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2α (Lutalyse™, dinoprost, tromethamine) 1 hour before semen collection, while in treatment 2 (P2), the semen collection was carried out 2 hours after PGF2α administration. ...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
...ring liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated exper...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...ervation in gaga chicken semen. The research material was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically an...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...as high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to September 2023. Semen diluted to a minimum of 25 million/mL is packaged in 0.25 mL straws. Treat Andromed® as a comparison, P0 tris egg yolk (TYE), P1 TYE+MYE 1 mL, P...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...influence of selenium on semen cytological parameters, 48 ejaculations were collected from 4 Kamohri bucks using an artificial vagina twice a week. Following the semen collection, ejaculates were assessed through macroscopic and microscopic evaluation. Semen ejaculates having ≥ 80% motility, morphology, and live-dead ratio were pooled and diluted in Tris-based Egg Yolk (TEY) extender su...
Makruf Arif1, Asmarani Kusumawati2*, Kurniawan Dwi Prihantoko3, Agustina Dwi Wijayanti4, Sri Gustari2
...oxidation during rooster semen cryopreservation reduces sperm functioning and creates reproductive issues. Antioxidants are crucial in semen cryopreservation to inhibit lipid peroxidase, thereby preserving sperm quality. This study aimed to investigate the effects of genistein and glutathione supplementation on the quality of cryopreserved sperm. Semen was collected from six KUB roosters a...
Nittaya Thongtip1, Sukanya Poolthajit1, Sirikhan Thartrak1, Qiongxian Yan2, Suntorn Wittayakun1*
...rformance and changes in semen quality. ...
Habib Asshidiq Syah1, Aulia Puspita Anugra Yekti1, I Putu Arya Girinata1, Ahmad Fahrudin Husen1, Rizki Prafitri1, Nurul Isnaini1, Nanang Febrianto1, Putri Utami1, Muhaimin Rifa’i2, Trinil Susilawati1*
...a single dose of unsexed semen of 100 before being exposed to FMD (T1) and 115 cows affected by FMD (T2) in Pujon subdistrict, Malang Regency, with a Body Condition Score 2.5-3.5 and aged 1.5-8 years and showing signs of estrus. Using the method of descriptive statistics. The percentage of AI success of T1 and T2 based on NRR-1 values showed no significant difference (P>0.05) 77.00% vs 86.96%, NRR-2 values showed no significant difference (P>0.05) 62.00%...
M. Ismail Chaudhry and Wali-ur-Rehman
...ee localities. Semanotus semenovi Okun, Anthaxia sp., Phloeosinus jubatus and Phloeosinus sp. were recorded feeding in bark and sapwood of dead and dying trees to various extent. Infestation of these was mostly restricted to Susnamana where the trees were weakened and killed by the attack of dwarf mistletoe (Arceuthobium oxycedri M. Bieb.), a plant parasite. Trichodes sp. Healthy trees in Sasnamana, Ziarat and Chautair were foun...
Mariana Isti Dwiningsih1, I Ketut Suatha2 and I Ketut Puja3*
...and viability of chilled semen on Kintamani dogs. The study used a completely randomized design, with four treatments and six replications. In experiment 1, sample examined immediately after added extender, experiment 2, 3 and 4 were. The results of the study found that the average motility percentages for semen transported at 0 hours, 1 hour, 2 hours, and 3 hours were 91.00% ± 0.89, 90.83% ± 0.75, 90.17% &plus...
Rwaida Adnan Ali1, Rahman Hussain Hamza¹, Hayder Mohammed Hassan Habeeb1, Ali J. Al-Nuaimi2
...onducted to evaluate the semen characteristics, testicular parameters, and blood parameters of three genetic groups of goat bucks. In this investigation, twelve mature bucks (average weight= 25 kg, average age= 2 years) were used: Cyprus (n=4), local black (n=4), and crossbreed (Cyprus X local black) (n=4). Semen and blood samples were collected from all groups every 2 weeks for 3 months. Semen
Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*
...mentation of post-thawed semen of Bali bull. This research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada. Seventy-five semen straws produced from one bull were divided into 3 groups; each group consisted of 25 semen straws as long as 3, 6, and 8 months of storage in liquid nitrogen. The quality of thawed
Husnurrizal Husnurrizal1,2, Hafizuddin Hafizuddin2*, Sri Wahyuni3, Cut Nila Thasmi2, Tongku Nizwan Siregar2,4
...lpha; administration and semen collection on the enhancement of spermatozoa quality, testosterone, cyclic adenosine monophosphate (cAMP), and adenosine triphosphate (ATP) levels. A total of 15 rats (Rattus norvegicus) categorized into five treatment groups (n=3) were used in this study. In the control group (P0), the semen and blood samples were collected 30 minutes after a 0.5 ml NaCL injection. In groups P1, P2, P3, and P4...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...lity, abnormalities, and semen membrane plasma were analyzed after thawing (%). C5 and E15 extenders resulted in higher total motility (p 0.05) than the other extenders, with the exception of C10 and C15, which were lower than that of the control. The extender E15 (P <0.05) exhibited the highest motility. Furthermore, compared with the other extenders, the C5 and E15 extenders led to better vitality and membrane plasma in post-thawed spermatozoa (p 0.05). F...
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
...f Tharparkar cattle bull semen. The research was carried out at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University Tandojam. In this study four fertile Tharparkar bulls (A, B, C and D) having age of 4-5 years were used. A total of 52 (n=13) ejaculates were collected with the help of artificial vagina, after the collection macroscopic (color, volume and pH) and microscopic (motility, morphology,...
Kusuma Adhianto*, Tina Rahmawati, Rio Ramanda, Muhtarudin Muhtarudin, Sri Suharyati
...ration) quality of sheep semen. The research involved an experimental study using a Randomized Complete Block Design (RCBD) consisting of 3 treatments, with each treatment being replicated 5 times. The treatments applied were as follows: P0; Complete Feed 60% + silage 40% (without the addition of natural and synthetic vitamin E), P1; Complete Feed 60% + Silage 40% + Natural Vitamin E 50 IU (equivalent to 223 grams of green bean sprouts), P2; Complete Feed 60% ...
Md. Zahid Hossain1, Md. Mahfuzul Haque2, Md. Masud Parvej3, Hemayet Hossain3, Faija Sadia Pory4, Dipsana K.C.5 and Md. Imranuzzaman5*
...among the others whereas semen percentages displayed not significant (p<0.05) among three groups. In addition, age of cow and variety of breed are also representing statistically significance (p<0.05) difference among the others as well as interrupted NRR. As the consistent growing livestock economy is grossly dependent on the success rate of AI, the Government should take much more research works to improve the non-return rate. Except for percentages of...
Mohammed Abdulameer Rashid Al-Sarray1, Hasanain Jihad Neamah2*, Sarah Gatea Fayyadh AL-Omairi1
...xtract (O.M.) in the ram semen at cooling, the experimental groups were divided into four groups (P1=1 mg O.M. raw powder and extender, P2= 2 mg O.M. raw powder and extender, O1 group= 1 mg O.M. oil and extender, O2 group= 2 mg O.M. oil and extender and the control only extender. The sperm motility, pH level, malonaldehyde level (MDA), and total antioxidant capacity (TAC) were investigated post-preservation (5ᵒ C) at 0, 72 hours of cooling, and after thirty ...
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
...native additives for the semen preservation process in different farm animal species to improve semen properties such as viability, motility, and membrane integrity, which thereby increase sperm fertility. The current study was conducted to investigate the effect of adding an aqueous extract of Aloe vera leaf gel (AEAV) to the epididymal spermatozoa of local goats during the cooling process for different periods. The AEAV wa...
Truong Thanh Trung1, Nguyen Thi Kim Dong2, Tran Long Hai1
...protein levels on rabbit semen characteristics. Thirty crossbred male rabbits (New Zealand White x local), with an average weight of 2.59 ± 0.14 kg, were completely randomized design consisted of five treatments and six replications, with one buck rabbit as an experimental unit. The five experimental treatments were diets containing 14%, 16%, 18%, 20%, and 22% crude protein corresponding CP14, CP16, CP18, CP20 and CP22 treatment, respectively. Feedstuff...
Sumaya Loay Mohamed Shams Al-Dean1*, Sura Shakir Hammoud2, Mohammedali J. Ghafil3
... on inferior-quality Ram semen. In this investigation, Four Ghezel-Merinose rams, who were roughly two years old, were employed .They were kept in settings of uniform lighting, housing, and food. The farm’s feeding programme was applied to the experimental animals as well. Ejaculate of the rams is divided into seven groups. For the investigation, only ejaculates with a weak motile function were chosen. Divided into four equal aliquots, each ejaculate was...
Khaeruddin1,2, Gatot Ciptadi1, Muhammad Yusuf3, Hermawansyah2, Sahiruddin3 and Sri Wahjuningsih1*
...cose, and fructose). The semen was diluted at a ratio of 1:10 and stored at 5 ºC for 48 h. The results showed that the type of diluent treatment affected abnormal morphology, progressive motility, local motility, total motility, immotil, linearity and straightness. In general, Ringer’s acetate-yolk glucose diluent is better in maintaining the quality of Gaga’s chicken spermatozoa during storage....
Al-Moataz Bellah Mahfouz Shaarawy1, Mahmoud Yassin Mohamed1*, Mahmoud Sayed Sayah2,1, Ashraf Ali Mehany1, Ezzat Arafa Ahmed El-Beltagi1, Shimaa M. Ali1
.... Also, sexual activity, semen characteristics and testosterone levels for bulls developed earlier than the 1st group. Heifers in the same group were superior in reproductive performance such as reaching puberty, and fertility compared to other groups. The 3rd group showed the highest concentrations of serum proteins, glucose, and lipid profiles. The 2nd group came next, and 1st group registered the lowest concentrations. In conclusion, data on GH and LP level...
Aulia Puspita Anugra Yekti, Ani Atul Arif, Putri Utami, Habib Asshidiq Syah, Anggita Dian Pramudhita, Rr. Ani Rizqianti, Trinil Susilawati*
...arch material used was a semen sample of Belgian Blue cross bull with progressive motility of fresh semen of≥ 70%. The research used a Completely Randomized Design (CRD) with four treatments and ten replications. Data were analyzed using the Tukey test. The treatment in the study was T0: sexing PDGC 10 fractions using Andromed® diluent. T1: sexing PDGC 10 fractions using tris aminomethane diluent + 20% egg yolk. T2: s...
Shimaa R. Hamam, Reham A. El-shafei, Nani N. Abdelaziz, Magdy S. Amer
...al analysis, analyze the semen profile and measure the activities of the enzymes glutathione-s-transferase and nitric oxide in the testicles. Testicular GST and NO activities, as well as the level of testosterone and sperm profile, were all significantly reduced while taking ibuprofen. Significant changes were also brought about in the testicular, epididymal, and seminal vesicle histological architectures. Important components of male fertility, such as testos...
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...e as a cryoprotectant in semen extenders is still underexplored. This study investigates the effects of antibiotic- and sugar-free Av-based extenders on cryopreserved tilapia sperm. Semen samples were collected and divided into four treatment groups: Control (C, 0% Av), Treatment 1 (T1, 10% Av, antibiotic: sugar), Treatment 2 (T2, 10% Av, antibiotic: sugar-free), and Treatment 3 (T3, 10% Av, antibiotic-free: sugar) mixed wit...
Adi Tiya Warman1, Panjono1*, Tri Satya Mastuti Widi1, Sigit Bintara1, Bayu Andri Atmoko2, Endang Baliarti3
...farmers in choosing bull semen for artificial insemination (AI) in Lombok Tengah Regency, West Nusa Tenggara, Indonesia. The bull semen choice was classified into three categories: Bali cattle, exotic cattle, and both. Respondent selection was carried out using a purposive sampling method, with 95 respondents. Data were collected through interviews using a structured questionnaire that had been tested for validity and reliab...
Abrar ul Haq1, Shusheng Yin2, Amara Maryam1, Muhammad Khan1*, Hafiz Abdullah Shakir1, Muhammad Akhtar Ali3, Muhammad Irfan4, Muhammad Faisal Maqbool1 and Yongming Li2*
Lalu Ahmad Zaenuri*, Rodiah Rodiah
...er buck spermatozoa. The semen used in this study was collected from a 3-year-old Boer buck weighing 85 kg, which was well-trained, fertile, and free of both internal and external parasites. Ten semen samples were collected using an artificial vagina every four days over a period of ten consecutive days. The collected semen was divided into four tubes based on to the treatment extenders: F...
Dheyab D. Radhi1*, Rasheed H.A. Al-Aidi2, Ali Ahmed Khalaf 3
...udy aimed to improve the semen quality of aged Awassi rams (local Iraqi sheep) by injecting L-carnitine directly into the venous blood. For this purpose, twelve rams with ages ranged between 5 and 6 years were used. The animals were distributed into three groups, each group consisted of four rams. The first group was given an intravenous injection of 5 ml of distilled water as a control group. The second group was given an intravenous injection of L-carnitine ...
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