The Quality of Bali Bull Sexed Sperms Using Freeze Dry Albumin at Different Concentrations of Sexing Medium
The Quality of Bali Bull Sexed Sperms Using Freeze Dry Albumin at Different Concentrations of Sexing Medium
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
ABSTRACT
Sexing spermatozoa using freeze dry albumin with different concentrations is expected to separate spermatozoa. This study aims to identify the quality and movement patterns in Bali bull sexed sperm using freeze-dry albumin. This study was conducted five times for semen collection and using treatments; T1 = Medium concentration of 10% and 30%, T2 = Medium concentration of 15% and 45%, T3 = Medium concentration of 20% and 60%. Parameters measured in this study were motility, viability, abnormality, membrane integrity, acrosome integrity, movement pattern and proportion of spermatozoa. The data obtained were analysed using one-way analysis of variance (ANOVA). A Descriptive Statistical test used to analyze the proportion of sperms. The results showed that different concentrations of sexing media showed no significant difference (P>0.05) on motility, viability, abnormality, membrane integrity, acrosome integrity, and movement patterns of spermatozoa in treatment between T1, T2 and T3 treatments both in the upper and lower fraction. In the movement pattern of sexed sperms, There was no significant difference in the kinematics parameters (P>0.05) in the T1, T2 and T3 treatments. The proportion of X:Y with freeze-dried albumin on the upper layer showed T1 (57.00:39.13) significantly different (P<0.05) from T2 (69.61:25.49) and T3 (75.00:24.00), while the lower layer showed T1 (33.17:64.36) significantly different (P<0.05) from T2 (23.27:72.77) and T3 (22.17:75.73). The proportion showed that X sperms in the upper fraction were higher than the lower fraction in all treatments, while Y sperm in the lower fraction was higher than X sperm. Based on the study’s results, it can be concluded that freeze dry albumin could be used as a sexing medium and maintain characteristics sperms with any concentrations, and can change the proportion of spermatozoa effectively from the natural proportion of spermatozoa.
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