Hu Zenglei1 and Xiufan Liu1, 2*
...justify; ">Virulence and pathogenicity of Newcastle disease virus (NDV) is one of the major determinants for the clinicopathological manifestations in infected hosts. Recent studies showed that the host innate immune responses to infection play important role in the pathologenesis of NDV, and correlate with the severity of the clinical disease. Virulent strains of different genotypes induce distinct pathological manifestations in chicken organs, especially in ...
Leonard Izzard1, 2, John Stambas1, 2*
...being used to manipulate pathogenicity and immunity and as biomarkers for disease severity....
Shen Yang1, Guang-Zhi Tong1, 2*
... life cycles. The highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) is a major concern for the swine industry and causes high degrees of morbidity and mortality. The intracellular signalling cascades of the innate immune system triggered by HP-PRRSV often lead to the elimination of infection. However, HP-PRRSV induces pathological changes and can cause aberrant immune responses in the host, resulting in acute lung injury and immu...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: [email protected]; [email protected]
...bat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different condi...
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
...V) is generally a noncytopathogenic virus in cell cultures. The development of reporter CSFV has provided an excellent option to directly detect viral replication without the use of secondary labeling. This mini-review discusses the applications of reporter CSFV in the study of CSFV biology, including efficiently screening the antivirals against CSFV and viral receptors, conveniently tracking the viral proteins in live cells as well as improving diagnostic met...
Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4
...gs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that the isolate clustered with the PPMV-1 strains recently reported from Israel within subgenotype VIb. Our findings report the emergence of PPMV-1 in Egyptian pigeons and the potential role the...
Neha Singh and Anjana Pandey
...erous cells, detect live pathogenic organisms, and to discover novel disease biomarkers to facilitate early detection of the disease and for therapeutic purposes....
John G. Bruno and Jeffery C. Sivils
...e surfaces of general nonpathogenic Escherichia coli (ATCC No. 8739) as well as alpha and gamma intimins and Shiga-like toxin 2 (SLT-2) B subunit verified that the aptamers bound proteins of the correct molecular weights for intact outer membrane proteins (OMPs), intimins and SLT-2. Some bands on the aptamer Western blots were shared between the “Big 6” non-O157 Shiga toxin-producing E. coli (STEC), E. coli O157 and related Gram negative bacteria. ...
Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5
...and application of entomopathogenic nematodes (EPN) depend on the use of host insects for in vivo production. As there is little information regarding relationship between nematode dosage and production of infective juveniles (IJ) of Steinernema feltiae and ability to locate hosts, therefore, in the present studies investigations were done on these aspects. A significantly greater emergence of IJ of S. feltiae from Galleria mellonella was observed with White t...
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...e a slum household-based pathogenic Leptospira population dynamics simulation model. The model will be analyzed to assess the role of slum-based biotic (human and animal) and abiotic (soil, water and weather) habitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have designed a pseudo-longitudinal survey to be undertaken for two years in Kibera slums in Nairobi city Kenya. Human and anima...
Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4
...malgam of beneficial and pathogenic microbes and its pH (6.7 to 7.4), high concentration of phosphorus (4.50% and 4.33%) and nitrogen (3.26% and 2.37%) favor seed germination, enhance root growth and soil fertility....
Muhammad Raheel1,*, Nazir Javed2, Sajid Aleem Khan2, Hafiz Muhammad Aatif3 and Sohail Ahmed4
... exotic species of entomopathogenic nematodes (EPNs) was checked on Galleria mellonella larvae. The native species included Steinernema asiaticum and Heterorhabditis indica whereas exotic species were S. feltiae and H. bacteriophora. G. mellonella larvae were exposed to 300 IJs of each species. After inoculation at different temperatures, the reproductive potential of EPNs increased with increasing temperature and was found to be the best at 25oC. No EPNs spec...
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...ccount the genotypic and pathogenic characterization of H9N2 in avian species. However little is known about the impact of different strains on the innate immune response. In the present study, using quantitative real-time PCR, cytokines gene expression were examined in response to infection with two strains of Egyptian H9N2 (namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains revea...
Waheed Anwar*, Muhammad S. Haider, Ahmad A. Shahid, Hamid Mushtaq, Usman Hameed, Muhammad Zia Ur Rehman and Muhammad Javed Iqbal
...d isolates. Additionally pathogenicity of isolated Fusarium species was evaluated against nymph and adult of Bemisia tabaci. Under controlled conditions different species of Fusarium restrained the growth of B. tabaci as compared with control.
...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...isolates indicating very pathogenic nature of infection in the area. Risk factors determinants were found significantly (P<0.05) associated with mastitis occurrence except frequency of milking per day. Antibiogram of Staph. aureus indicated very strong resistance to oxacillin, ticarcillin, ampicilline, amoxicillin, azlocine, chloramphenicol, mupirocin, vancomycin, cefixinme, cefuroxime, and cefotaxime. In contrast to this sulphaphenazole, gentamicin,...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
...a (PED) was triggered by pathogenic coronavirus causing watery diarrhea and high mortality in piglets. This post-viral syndrome results in severe economic losses in the pig industry. However, limited information on the porcine epidemic diarrhea virus-infected pigs is available. Thus, the objective of this study was to identify transcriptomes with RNA sequencing (RNA-seq) in peripheral blood mononucleated cell (PBMC) of piglets, Large White x Landrace crossbred...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...align: justify;">The low pathogenic avian influenza viruses (LPAIV) subtype H9N2 are highly prevalent in the Middle East and are causing economic losses in the commercial poultry production sectors. Due to potential of H9N2 infections in human, LPAI H9N2 poses serious public health risk. Therefore, development of new potent and cost-effective influenza vaccines is urgently needed to safeguard poultry and to contain zoonosis. The primary goal of this study was ...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
...rofuse root galling. The pathogenic potential of M. incognita on passion fruit was determined; the minimum 10 second stage juvenile (J2)/200 cc soil can cause reduction of plant growth parameters and highest reproduction (R=87.27).
...
Neenish Rana, Nosheen Ehsan, Awais Ihsan and Farrukh Jamil*
...genome sequence of human pathogenic bacteria Helicobacter pylori (H. pylori) strain 26695. Computational analysis of the genome showed 6 transcriptionally active pseudogenes that can produce stable mRNA secondary structure compared to their functional parents. Moreover it was observed that their putative protein products will be thermodynamically stable. The sequence-based predictions suggested that the pseudogenes-derived proteins may involve in...
Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram
Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies
...olates belonged to plant pathogenic genera like,Alternaria, Cercospora, Colletotrichum, Curvularia, Glomerella, Mycosphaerella, Phoma and Stemphylium and the other 24 isolates of fungi belonged to Aspergillus, Acremonium, Chaetomella,Cunninghamella, Geotrichum, Gliocladium, Monodictys, Mucor, Myrothecium, Penicillium, Periconia and Pithomyces. One-hundred and thirteen isolates of chilli microflora and 49 isolates of Trichoderma, 19 isolates of Bacillus sp., 34...
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...mber of soil-borne plant pathogenic fungi, suppressive effects on some root nematodes without adversely affecting beneficial microbes like Rhizobium and capable of promoting growth of certain crops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production ...
Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha
Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...ey, one isolate of entomopathogenic nematode from ginger rhizosphere was collected from Faizabad district of Uttar Pradesh (India). Morphological and morphometric studies identified the isolate as Stein-ernema carpocapsae. This was further confirmed by ITS-rDNA sequences analysis. Phylogenetic was constructed for studying relationship with known isolates. Pathogenic potential of the isolate of S. carpocapsae (IISR-EPN 06) on...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...l changes in response to pathogenic field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...tibodies production. The pathogenicity and etiology of the disease has yet to be elucidated. It is presently accepted that environmental factors trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a number of autoimmune diseases. We hypothesized that gluten containing diet would further exacerbate an already undergoing arbitrary immune reaction in SLE pa...
Gadeeyya G and Ratna Kumar P.K.
...relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed.
...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...>The incursion of highly pathogenic avian influenza virus (HPAIV) strain H5N1 in 2006 in Egypt caused severe economic losses for both commercial and backyard poultry production sectors. Owing to public health risk, effective control of the virus in imperative. DNA vaccination is a promising new approach for the prevention and treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...ify;">Brucella is pathogenic bacteria that cause animal and human brucellosis. Currently, the mechanism behind the pathogenesis of Brucella remains unclear. For this reason omp10 mutant was constructed to examine the impacts of the outer membrane protein 10 (Omp10) on bacterial survival, virulence, phagosome-lysosome fusion, and apoptosis induction, as assessed in appropriate in vitro (cell culture) and animal models. Results showed...
Jawad Anwar* and Zafar Iqbal
... against phyto and human pathogenic bacterial strains. T. harzianum was grown under different conditions and their extracts were evaluated, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Escheri...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...asma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 2...
Zahid Latif1,*, Kathrin Blasius2-4, Tufail Hussain Tahir7, Muhammad Nasim Khan1, Ghazanfar Ali5, Ansar Ahmed Abbasi6, Abdul Rauf1, Hao Hu8 and Angela M. Kaindl2,3,4
...B1 gene as an underlying pathogenic variant for non-syndromic autosomal recessive retinitis pigmentosa.
...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
...rmine the prevalence and pathogenic potential of Escherichia coli (E. coli) isolated from piglets having white score diarrhea as a result of outbreak occurred in Qinghai Tibetan Plateau in 2015. A total of 81 E.coli were isolated from 83 fecal samples. The organisms were inoculated on MacConkey agar and EMB agar and identified via biochemical tests. Polymerase chain reaction was used to detect representative virulence factors or genes, including ...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...revalence of potentially pathogenic intestinal protozoan parasitic infection in farmers, education concerned and shepherds of Swat, Khyber Pakhtunkhwa, Pakistan. A total of 1041 stool samples were examined from January 2006 to December 2008 using direct smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with singl...
Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
...binary mixtures of entomopathogenic fungi, Beauveria bassiana (isolates Bb-01, Bb-10), Metarhizium anisopliae var. anisopliae (isolate Ma-11.1, Ma-2.4)and Isaria fumosorosea (isolates If-2.3, If-02) and chemical insecticides i.e., bifenthrin, lambda cyhalothrin, imidacloprid, triazophos, spinosad, pyriproxyfen and nitrinpyrum, as larvicides against C. pipiens. Highest larval percent mortality (73.3 ± 4.7) was ob...
Ayesha Aihetasham*, Faiza Qayyum and Muhammad Xaaceph
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
... additive or synergistic pathogenic effect in the reproduction of respiratory disease if given simultaneously or three days apart.
...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
...of potentially important pathogenic parasitic infections particularly in remote parts of the country.
... 
