Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...increasing incidences of antimicrobial contamination, especially in food, dairy products, agriculture and environment, their regular monitoring is on prime interest. Aptamers are synthetic short sequences of single stranded (ss) oligonucleotides (ss-RNA or DNA), which are developed by an in-vitro selection process known as “Systematic Evolution of Ligands by Exponential Enrichment (SELEX)” technique. Among the receptors available for biosensing, ap...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
... was used to monitor the antimicrobial activity of these strains both in mono culture and mixed culture. Streptomycin sulphate (10 μgml-1) and Amphotericin B (5 mgml-1) were used as positive controls. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts was also determined. Syzygium aromaticum was proven to have excellent antimicrobial activity against all tested microorgan...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
...rious fungal species for antimicrobial natural products. During this study five different fungi, including Alternaria alternata, Acremonium sp., Alternaria brassicicola, Pythium sp. and Aspergillus flavus were screened against Wilt causing pathogens using the dual culture assay. Among them, Aspergillus flavus was found active fungus against the selected vascular Wilt causing fungus and bacteria, i.e. Fusarium oxysporum (87.50 ± 0.11% inhibition), Xantho...
Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...s and subsequently their antimicrobial susceptibility.The milk samples were screened with surf field mastitis test and furthered to biochemical analysis. Pearson’ Chi-square test at nighty five percent confidence interval was used to analyze the collected data. Antibiotic sensitivity was checked with twenty four antibiotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on o...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...ng the light on emerging antimicrobial resistance that represent major concern for public health. A total one hundred swabs and 500 organ samples were collected from apparently healthy and freshly dead quails respectively. Bacterial isolation and characterization were performed in accordance with the clinical laboratory standards and confirmed by PCR. In life birds, only E.coli and Salmonella could be recovered from Cloacal swabs, while in freshly dead birds a...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
...es on the occurrence and antimicrobial resistance profile of Salmonella in poultry slaughter slab (processing plants) in Nigeria. The relatively high prevalence rate documented in this study may be attributed to the generally poor infrastructure, lack of well-equipped poultry slaughter houses, lack or inadequate water supply at these markets which hampers the ability of handlers to maintain good sanitary and hygiene conditions of the carcass, environment and t...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
...stify;">Mankind is using antimicrobial agents in various forms since last 2000 years. The present study was designed to evaluate the antimicrobial activities of different culture microbes against each other. A total six bacteria including Salmonella typhi, Escherichia coli, Pasteuralla multocida, Lactobacillus bulgaricus, Micrococcus luteus and Staphylococcus aureius were used against each other to evaluate the...
Anil Sehajpal, Saroj Arora and Parminder Kaur
Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
... showed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity even at the lowest concentration, i.e. 100 ppm, with relative magnitude of...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...mandisplays a variety of antimicrobial, antioxidant and immunomodulatory as well as a number of other biological functions. Its potential can be exploited in several food applications. Keeping in view the increased demand of natural antimicrobial agents to control prevalence of food borne pathogens in final packaged food products as well as for food preservation/ biocontrol, this study was designed to isolate lactoferrin fro...
Saba Irshad1,*, Ayesha Ashfaq1, Ammara Muazzam1 and Abida Yasmeen2
...e been used as an active antimicrobial compound since long. The present study was intended to validate the antimicrobial, antidiarrheal and anticancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.), used in Pakistani cuisine to enrich flavour and to treat infectious wounds. Their crude ethanol and methanol extracts were tested against five important bacterial and fungal strains. Both...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...ntific evidences for its antimicrobial effects particularly against NDM-1 producing bacteria are lacking. In addition to antibiotic profiling, the present study aimed at determining the in vitro anti- bacterial activity of Manuka honey against NDM-1 producing clinical isolates of Pakistan. A total of five carbapenem resistant bacterial species; E. coli, K. pneumoniae, E. cloacae, A. baumannii and P. aeruginosa, were isolated and identified...
Hafiz Muhammad Tahir1, Khanum Zahra2, Arooj Zaheer2, Khizar Samiullah3
...ssed. Its antifungal and antimicrobial potentials are also explored. Researchers are trying to introduce advanced methods to manufacture spider silk artificially to enhance silk production. Silk fiber can be designed after determining type and sequence of constituent amino acid or by inserting silk producing gene in another suitable animal....
Rahila Nazli, Tehmina Sohail, Bushra Nawab and Zahra Yaqeen*
...> Quart for the new antimicrobial agent is still there and the present work is an attempt in this regard, Ethanolic extract of Gliricidia sepium in different concentration was used to investigate its antimicrobial activity against gram +ve and gram-ve bacteria. The study was also extended to some species of fungi. Results showed that the activity was more pronounced against gram +ve organisms and fungi. Maximum inhibiti...
Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...s aimed to determine the antimicrobial resistance of E. coli isolates from bovine mastitis and identification of antimicrobial resistance and virulence genes associated with them. Antimicrobial susceptibility testing was performed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% ...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...istance to optochin. The antimicrobial screening of Streptomyces against S. mutans showed that these strain are the prolific producers of active agents to inhibit the growth of caries causing S. mutans. The natural extracts of Streptomyces strains significantly inhibited and delayed the biofilm formation by S. mutans and a profound effect was observed in case of the extracts of Streptomyces strains HU-09 and HU-10, whi...
Nina Bao1,*, Yongguang Yin2 and Panpan Wang3
...leum ether to extract an antimicrobial cerebroside from the liposoluble constituent of cervus nippon antler velvet layer was studied in this paper. Single-factor experiment was used to research the effect of liquid-to-solid ratio, standing time and centrifugation time on the extraction of crude cerebroside. The optimal extraction conditions for the cerebroside as follows: liquid-to-solid ratio 15, standing time 45 min, and centrifugation time 6 min. The extrac...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...as further processed for antimicrobial susceptibility testing by modified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative).
Results:...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...nanoparticles to enhance antimicrobial activity against these pathogens.
...
Asinamai Athliamai Bitrus1*, Olabode Mayowa Peter2, Muhammad Adamu Abbas3 and Mohammed Dauda Goni4
...stify;">The emergence of antimicrobial resistance in Staphylococcus aureus posed a major veterinary and public challenge worldwide. S. aureus being a highly versatile pathogen can quickly acquire resistance genes. The development of resistance in bacteria predates the era of antibiotic use. However, resistance developments in S. aureus have been reported since the early 1940-ties, when penicillin resistant S. aureus was first reported. Ever since, this pathoge...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...ound to have significant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse metabolic compounds. The study predicts that the seactinomycetes strains are a potential resource to cope up with evolving multi drug resistant pathogens. Furthermore, these secondary metabolites can be identified by comparative studies and these actinomycetes ...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
Asma Sohail1*, Aina Rafique2, Kashif Sarfraz Abbasi1 and Maryum Arif3
...dants and have potential antimicrobial activity. Effect of drying methods (sun-, oven- and freeze drying) on antioxidant and antimicrobial activities of date fruit powders from three varieties Zahidi, Aseel and Muzafati were investigated. The DPPH activity was significantly higher in Muzafati sundried date powder, whereas maximum phenolic and flavonoid contents were significantly higher in Muzafati freeze dried date powders....
Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1
Amjad Islam Aqib1,*, Shagufta Nighat2, Rais Ahmed3, Saba Sana3, Muhammad Ameen Jamal4, Muhammad Fakhar-e-Alam Kulyar2 , Naimat Ullah Khan5, Mian Saeed Sarwar5, Muhammad Asif Hussain5, Asadullah5, Attaur Rahman5 and Sadeeq ur Rahman5,*
...factors, involvement and antimicrobial susceptibility of Staphylococcus aureus in the development of SCM in District Faisalabad of Pakistan. For this purpose, a total of 384 goat milk samples were screened for SCM through surf field mastitis test (SFMT) and mastitis-positives cases were further investigated for isolation of S. aureus using standard procedures. Coagulase gene was PCR amplified from the clinical isolates to categorize them into Coa...
Dilara Abbas Bukhari1, Abdul Rehman2*
Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...mycelium was checked for antimicrobial activity against bacterial strain, Bacillus subtilus. Biochemical techniques, thin layer chromatography, high performance liquid chromatography and gas chromatography/mass spectrometry, were employed to study fungal exudate. A total of fifteen different metabolites were detected in the sample by GC/MS. The detected metabolites could be classified into chemical groups of 2 esters, 4 alcohols, 3 benzoic acids, ketone, aldeh...
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ermine the frequency and antimicrobial susceptibility patterns of MBL producing carbapenem-resistant Gram-negative rods (GNRs) isolated from clinical specimens. A clinical descriptive study was conducted in Faisalabad, Pakistan from June 2016 to January 2017. A total of 152 clinical samples were processed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics as per Clinical and Laborat...
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
...rce for discovery of new antimicrobial drugs. This study was carried out, include in-vitro antibacterial and antifungal screening of aqueous methanolic extracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.0...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
...d to be 39.46 mg/ml. The antimicrobial activity was assessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.
...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...his study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by disk diffusion method. Double-disk synergy test (DDST) was also performed for detection of Extended Spectrum β-Lact...
Sadaf Rahim and Mudassar Iqbal*
...condary metabolites with antimicrobial, herbicidal, insecticidal and other such beneficial activities. Production of such compounds can be increased or decreased depending on growth conditions. For these reasons a Trichoderma harzianum was selected for its growth optimization conditions and extraction of insecticidal compounds effective against two major insects pests including Diuraphis noxia and Tribolium castaneum. The pure fungal strain of Trichoderma was ...
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
...has exhibited remarkable antimicrobial activity against certain strains of bacteria and yeasts. In general, significant antimicrobial activity was recorded against gram positive bacteria (zone of inhibition: 16.3±0.35–30.6±0.11 mm; MICs: 1–4 μg/ml); while gram negative bacteria (zone of inhibition: 10.1±0.23–17.2±0.13 mm; MICs: 4–16 μg/ml) and yeasts (zone of inhibiti...
Muhammad Naeem Khan* and Asad Jan
...t fractions and in-vitro antimicrobial activities of whole plant of D. botrys. Qualitative phytochemical screening of MCE and solvent fractions of D. botrys showed the presence of alkaloids, phenols, flavonoids, saponins, tannins and sterols, however in n-hexane fraction (HxF) only flavonoids and saponins were detected. In quantitative analysis, among all the solvents, ethyl acetate fraction (EAF) had highest amount of phenol (27.4 mg/g), flavonoids (15.5 mg/g...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...otential in synthesizing antimicrobial agents. In the present research work, four fungal strains Rhizopus stonolifer, Aspergillus niger, Aspergillus fumigatus and Aspergillus terreus were tested for the production of antimicrobial agents. The produced antimicrobial metabolites included Aflatrem, Monacolin K, Fusarubin and Tautomycetin. Different fermentation par...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
... to perpetual increasing antimicrobial resistance, functionally substituted alicyclic compounds are envisioned as probable antimicrobial agents of future. From screening of in house library of compounds, here we report the antimicrobial properties of three novel functionally substituted monocyclic and spirocyclic cyclohexane derivatives involving ethyl-3 (allylamino)-9,9-dimethyl- 7,11-dio...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...s proven anti-fungal and antimicrobial activities also detoxify Paraben (suspected car-cinogen and endocrine disruptor) instigated damages.
...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...iv>...
Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*
...s aimed to enlighten the antimicrobial and cytotoxic activity of Haplophyllum gilesii (Hemsl.) C.C. a narrow endemic plants of northern areas of Pakistan. The antimicrobial potential of H. gilesii extracts in different solvents was assessed using agar well diffusion method against bacterial and fungal strains, while cytotoxic activity was studied in the methanolic extract using brine shrimp’s lethality assay. All the e...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...igate the occurrence and antimicrobial resistance of this pathogen, a total of 360 chicken cloacal swabs and 15 water samples were gathered from different localities in Giza and Cairo Governorates. An additional 50 stool specimens were collected from individuals in contact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (P...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...sis of their preliminary antimicrobial activities. The isolated actinobacterial strains showed an optimum growth at alkaline pH (9.0-9.5). The taxonomic analysis revealed that these isolates were closely affiliated with the genus Streptomyces (62.5%), Lentzae (6.25%), Pseudonocardia (6.25%), Lechevalieria (6.25%) and Nocardiopsis (12.5%). Most of the isolated strains exhibited strong inhibition against a broad range of pathog...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
...solates were studied for antimicrobial resistance. Presence of bla NDM was determined by polymerase chain reaction. Among 240 strains, prevalence of MBL in K. pneumoniae, P. aeruginosa, E. coli and Acinetobacter Species was 26%, 52%, 27% and 5% respectively. Prevalence of blaNDM-1 gene was 51% identified in 14 strains of P. aeruginosa, 2 strains of A. Baumanni, 29 strains of E. coli and 18 strains of K. pneumoniae. In conclusion, high prevalence of blaNDM-1 in...
Mohammad Ejaz1, Salma Javed2, Muhammad Hamza1, Sadia Tabassum2, Muhammad Abubakar3, Irfan Ullah4*
...molecules can be used as antimicrobial, antibacterial and anticancer agents. Fungal endophytes like Taxomyces andreanae isolated from Yew plant have the potential to produce an anti-cancer compound called Paclitaxel. Other different fungal endophytic species produce various other types of anticancer compounds like camptothecin, podophyllotoxin, torreyanic acid, vincristine, and vinblastine. This review is organized to describe the general study of natur...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
...esent study investigates antimicrobial activities of different solvent extracted samples of Ziziphus mauritiana var. spontanea and Oenothera biennis against different microbes. Ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea measured the highest zone of inhibition against Staphylococcus epidermidis, Escherichia coli and Salmonella typhi. The MIC values of ethyl acetate and ethanolic extracts...
Sana Khan1, Raheela Taj1, Noor Rehman2, Asad Ullah3, Imad Khan3 and Sadeeq ur Rahman3,*
...beta;-lactamases and antimicrobial susceptibility profile. Our results indicated that a total of 130(4.40%;130/2950) samples were positive for C. freundii comprised of urine 58.46%, pus 33.85% and wounds 7.69%, respectively. More samples from females (n=70) than males (n=60) with majority (43.08%) from age group 21-40 years were found positive for C. freundii. Of the 130 isolates, 28 (21.53%) were ESBL producers comprised of 10 (35.71%...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2, Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...tion of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX (27%), CIP (25%), LFX (13%) and AZM (11%) while the leas...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3, Muhammad Ijaz Saleem1, Muhammad Shoaib4, Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...and heightened change in antimicrobial resistance by all isolates which demands immediate action plans to be taken.
...
Ayesha Talib1,2, Khanzadi Nazneen Manzoor1, Wajahat Ali1, Maria Saeed1, Muhammad Asif Gondal1, Malik Badshah3 and Abid Ali Khan1*
...al properties as well as antimicrobial activity against Multidrug Resistant (MDR) bacteria is evaluated. The total formation of the Cu nanoparticles was observed visually with a color change and confirmed by the appearance of peak through UV-Vis spectroscopy analysis. The electron microscopy imaging revealed that the biogenic Cu nanoparticles were spherical in shape and had a diameter of 30-40 nm. The synthesized nanoparticles showed promising antibacterial ac...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3
... laboratory. Alarmingly, antimicrobials in animals are inappropriately using and resultantly leading to antimicrobial resistance (AMR) including mycoplasmas, which are causing serious infections in ruminants. Since the growth curve of our local strain and its susceptibility to commonly using antimicrobials is unknown. Therefore, this study was aimed to evaluate the growth curve and suscept...
Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5
Preparation and Quality Evaluation of Soymilk Carrot Blend
...ds, protein contents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and carrot supplemented soymilks remained acceptable up to 7 days at refrigerated temperature. Plain soymilk had lower TPC at both temperatures than carrot supplemented milk. pH, TSS, carotenoids and protein contents in samples were decreased with increased time at both the temperatures. Highest protein and carotenoid content was detected in 6% carrot...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
...nown as biofilm. Various antimicrobial approaches used to treat gastrointestinal infections are usually ineffective due to biofilm formation. The purpose of this study was to (1) compare biofilm formation of three S. Gallinarum strains isolated from commercial broiler chicken by three different methods i.e., congo red, test tube and air liquid interface coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm ...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
... efficacy of the various antimicrobials (9 types) on the isolated M. bovis by using different antimicrobial sensitivity plate method. A total number of 100 samples of mastitic milk from native lactating buffaloes’ breed were collected aseptically from 4 private farms in Cairo-Alexandria desert road in the north part of the Egypt and subjected to bacteriological and molecular examination for M. bovis follo...
Waseem Khalid1, Ali Ikram1*, Muhammad Rehan2, Farukh Adeem Afzal1, Saadia Ambreen1, Marryam Ahmad3, Afifa Aziz1 and Anam Sadiq1
...nts, hypoglycemic agent, antimicrobial, anti-genic and antioxidant potential.
...
Basharat Ahmed1, Muhammad Hamza Azhar2, Farah R. Shakoori1 and Abdul Rauf Shakoori1,3,*
...="font-size: small;">The antimicrobial susceptibility patterns of 146 Campylobacter spp.isolated from diarrheal patients admitted to hospitals in Azad Kashmir Pakistan were analyzed to determine their changing trends in response to fifteen antibiotics. Campylobacter isolates were identified as C. jejuni (66.4%) and C. coli (33.6%). An over whelming majority of isolates were recovered from children (72%), 52 % were from male patients...
Muhamamd Rizwan1*, Muhammad Arshad2, Muhammad Kashif3, Aneela Zameer Durrani4, Asghar Abbas5, Tanveer Ahmad6, Muhammad Nadeem7 , Kinza Khan8
...ibit the permeability of antimicrobials from host body into the bacterial cells. CRISPR Cas system also help bacteria to evade from the host immune system by suppressing the activity of their immune receptors e.g. TLR. CRISPR Cas system also help bacteria to attach with the host body and replicate with in host body. It can develop antibiotic resistance in pathogenic bacteria, enhance their pathogenicity and can survival in host body.
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...ried out by API 20E. The antimicrobial susceptibility profiling and phenotypic confirmation of ESBL producers were performed as per CLSI 2018 guidelines. The minimum inhibitory concentration of ESBL producing isolates was determined by the broth dilution method. Phenotypically confirmed ESBL producing strains were further subjected to molecular characterization for the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL posit...
Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski
...ion is the change in the antimicrobial resistance of this microorganism towards some critical antimicrobials used in the human and veterinary medicine. In this study samples were taken from three points in the broiler meat production (farm, slaughter line and cold storage of the meat before shipping to the market). A total of 283 samples (cloacal swabs, caeca and carcass swabs) were analyzed for the presence of C. jejuni...
Md. Sahidur Rahman1*, Tishita Ape1, Md. Islam1 and Sharmin Chowdhury2
...ds to the development of antimicrobial-resistant microorganisms. We conducted a cross-sectional study to assess the perspective of dairy farmers regarding antibiotic use as well as its residual and resistance effect. We collected data from 101 dairy farmers in Chattogram, Bangladesh. The results revealed that ceftriaxone, gentamicin, streptopenicillin, amoxicillin, and sulfur drugs were the most frequently used antibiotics in the study area. Among the particip...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...x;">and characterize the antimicrobial profile of Salmonella spp.beef meat samples were collected from the local butcher shops (n=75) of Karachi, Pakistan. Three types of meat samples were collected viz., muscles, lymph nodes and minced meat (n=25 each). The samples were packaged aseptically and transported to laboratory within 4-6 hs of collection in cooling conditions. The samples were examined for Salmo...
Phytochemical and Antimicrobial Potential of the Roots of Berberis brevissima Jafri. (Berberidaceae)
Zain Ullah1*, Anwar Ali Shad1 and Saqib Ali2
Mona Kadry, Sara Mohamed Nader*, Esraa A. Elshafiee and Zeinab S. Ahmed
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...usceptibility to various antimicrobial agents including the aminoglycosides was determined by the Kirby-Bauer method and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sanger sequencing. The isolates showed a high rate of resistance to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were...
Murat Onur Yazlık1, İnci Başak Müştak2*, Gültekin Ünal3 and Ufuk Kaya4
Manar M. Farouk1*, Amal El-Molla1, Fayez A. Salib1 and Yousef A. Soliman2
... over the counter use of antimicrobials, lack of disinfection of feeding utensils and water troughs, and presence of rodents in feed storage area. Enterotoxin (stn) gene was detected in all recovered salmonellae, and the phylogenic analysis of stn gene of the selected strains and the retrieved sequences from GenBank showed the relatedness of the isolated strains to the other strains isolated from different sources. Accordingly, the analysis of di...
Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3*
...nosa is harbor multidrug antimicrobial resistance of camel has serious consequences for human health, so, this study aimed to characterized of P. aeruginosa especially extended spectrum β-lactamases (ESBL) producing one; phenotypic and genotypic. The isolates of P. aeruginosa were confirmed biochemically by a Vitek 2 compact system (bioMe´rieux). Suspected P. aeruginosa colonies carry ESBL were 56.6% by the double disc synergy test (DDST). Detection...
Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2*
Hailemariam Assefa1*, Tesfahun Dagnaw2
...o determine the in vitro antimicrobial activities of Neem seed oil by r disk diffusion method. Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petrole...
Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*
... in combating the global antimicrobial resistance threat....
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
...pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Salmonella with the acquisition of shish-tawook (14%). On the other hand, 100 hand swabs that were assembled from food handlers working in the same places yielded 13 Salmonella isolates, at the time 4 isolates were only obtained from 50 h...
Xiaoli Cai1,2, Saeed Ahmed3, Zhixin Lei1,*, Jianping Wu1,* and Taolei Sun1,2,*
... targeting bacteria with antimicrobial drugs and resistance to multidrug (MDR). The present study delivers a possible elucidation to this problem via Nano Drug Delivery. Nanomaterials are relatively small as compared to ordinary antibiotics. The nanoparticle has unique physical and chemical properties, a large surface area, and an electric charge. Additionally, its antibacterial drug encapsulation can make it easier to enter the cell and improve the concentrat...
Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3
...ositive for E. coli. The antimicrobial susceptibility testing of 72 confirmed E. coli isolates against seven quinolones and fluoroquinolones antibiotics revealed that 62 (86.1%) were resistant to at least one antibiotic, with highest resistance rates observed against first generation (86.1% for nalidixic acid, and 81.9% for flumequine), and lowest rate against levofloxacin (33.3%). A total of 24 isolates, dsplaying high resistance to at least 5 fluroquinolone ...
Hams M.A. Mohamed1, Mona A. El-Zamkan2*
...ptation to new hosts and antimicrobial resistance, resulting in the spread of infection all over the world, leading to huge health loss. A total of 100 raw milk samples collected from small scale producers, farmers and markets at Qena city, Egypt, were examined for the presence of Streptococcus species. The preliminary identification was confirmed by using VITEK®2 system which revealed the presence of four isolates of S. thoraltensis. The
Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1*
Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*
...s antibiotic residue and antimicrobial resistance. Alternative feed additives should be developed to replace the use of AGP in poultry production. Hence, the study aimed to evaluate the effects of combined dietary probiotics L. lactis D1813 and B. licheniformis D3270 on broiler performance, energy utilization, intestinal morphology, and microbial population. A total of 600 one-d-old Lohmann broiler chicks of 300 males and 300 females were used and reared for 3...
Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2
...animals. The response of antimicrobials against this infection is very poor that usually lead to culling of infected animals. In Pakistan, unfortunately no data is available on the occurrence of mycoplasma mastitis in farm animals. Therefore, current field investigation was conducted first time in centre-west Balochistan to get the preliminary data on mycoplasma mastitis. Milk samples (n = 579) were collected from three districts viz., Jaffar abad, Sohbat pur ...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...ndlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of chicken and beef meat and human. Bacteriological culture and PCR were used for S. aureus detection in 150 meat products (50 grilled chickens, 50 grilled beef kofta, and 50 cooked beef meat) as well as 92 food handlers. 23S rRNA gene sequencing was done. Disk diffusion method was used for
Hafiza Mehwish Iqbal1, Qurrat Ul Ain Akbar1, Saqib Arif1, Shahid Yousaf2*, Salman Khurshid1, Saqib Jabbar2, Neelofar Hamid3 and Uzma Sitara1
...cryl hydrazyl (DPPH) and antimicrobial activity in Roma VF tomato variety was assessed within three different mature levels viz., green, pink and red. Outcomes from recent study pointed out that maturity levels involved to change the quality of tomato. Moisture and ash increased with the maturity ranged from 89.38±0.54 to 93.34±0.48 and 0.35±0.02 to 0.41±0.02% respectively. pH increases 4.23±0.15 to 4.77±0.15 and % Tit...
Asmaa Sh. Fayed1*, Safaa M. Abo El-Soud2
...lm as an antioxidant and antimicrobial food packaging material, focusing on the quality, shelf life, and antioxidant properties of veal meat chunks during refrigerated storage. After coating meat samples, we analyzed their microbial, chemical, and sensory properties after 0, 3, 5, 7, 9, and 11 days of chilled storage. GA/ zein wax sheets significantly reduced (p < 0.05) the abundance levels of total bacteria, and psychrotrophic bacteria, as well as pH, thio...
Samah Samir, Amal Awad*, Gamal Younis
...n was used to test their antimicrobial susceptibility, and PCR was used to screen E. tarda isolates for the presence of four virulence-related genes (cds1, edwI, qseC, pvsA). In addition, the ability of E.tarda to form biofilm was tested by tube test. E.tarda was detected in 15 fish (6%) and a total of 40 isolates were recovered from organ tissue samples and confirmed based on phenotypic and molecular characterization. The frequency of edw1, cds1, qseC and pvs...
Stephen Chijioke Emencheta1, Anthony Amaechi Attama2, Ezinwanne Nneoma Ezeibe1, Adaora Angela Agubata1 and Ebele Benedette Onuigbo1*
Nabila Khan1, Imran Ahmad2 and Muhammad Bilal Sadiq1*
...mond hull extract showed antimicrobial effect against Escherichia coli, Staphylococcus aureus and Salmonella typhimurium. β-sitosterol (44 %), was identified as major phytoconstituent in almond hull extract. Due to antioxidant and antimicrobial potential, almond hull extract can be utilized as a functional food ingredient, and natural preservative.
...
Rasha Ali Taha Hamza1, Atef Saad Osheba1, Hassan Mohamed Sobhy2, Sahar Hussein Abdalla Hekal2*
...mine the antioxidant and antimicrobial activities as well as total phenolic and flavonoids contents in both water and ethanolic extracts of galangal and sumac extracts. Also, the effect of previous extracts on quality attributes of beef burger was evaluated. The ethanolic extracts of both galangal and sumac were shown to have significantly higher total phenolic and flavonoid contents than the aqueous extract, in particular 90% Ethanol Extract, where the 90% Et...
Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5
...tion of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63.75%), AMP (58.75%), SXT (50%), CHL (48.7%), CLR (36.25%), STR (25%), GEN (17.5%), OFX (15%), LFX (12.5%), AZM (...
Mohammad Belal Shaker1, Waleed Rizk El-Ghareeb2,3*, Marwa Magdy Seliem4, Wageh Sobhy Darwish3, Bassam Abdulla Alhawas2, Ahmed E. Tharwat3
... using PCR. Furthermore, antimicrobial resistance profiling was examined among the recovered A. hydrophila isolates. The obtained results of the present study revealed overall isolation rates of Aeromonas spp. at 48.33%, and 21.66% in the examined crustacean samples collected from Egypt and Saudi Arabia, respectively. Crab samples had the highest prevalence rates, followed by lobster, and shrimp in samples collected from Egypt and Saudi Arabia, respectively. F...
Emtenan M. Hanafi1*, Fouad M. Tawfeek2, Walid S. El Nattat1, Moetazza M. Alshafei3, Seham S. Kassem3, Kawkab A. Ahmed4, Enas N. Danial5, Hagar Elbakry3, Hoda S. El-Sayed2
...henolics, DPPH, HPLC and antimicrobial test against different types of bacteria were done for SP extract. BL has been microencapsulated in association with SP. Twentiy eight. adult male rats were divided into 4 equal groups. G1 received high fat diet (HFD), G2 received HFD and microencapsulated BL, G3 was supplemented by HFD plus encapsulated SP and BL. While G4 received basic diet as a control. After 2 months, animals were sacrificed. Results showed that SP h...
Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby*
...nsidered the lastly used antimicrobial drug for the treatment of many infections. As the extensive use of colistin, resistance has been increasing rapidly, thus, 200 different chicken samples were examined for isolation of E. coli and for colistin resistance gene. Eighty-three selected samples (chicken breast) were subjected to detection of colistin residues by HPLC (High-performance liquid chromatography). In this research, we used polymerase chain reaction (...
Iram Liaqat1*, Umaima Bibi2, Muhammad Arshad3 and Najma Arshad2*
...ith natural and inherent antimicrobial and antioxidant properties....
Sri Melia1*, Indri Juliyarsi1, Yulianti Fitri Kurnia1, Evy Rossi2, Hurriya Alzahra3
....3% bile salt media, and antimicrobial activity against Escherichia coli: O157, Pseudomonas, Listeria inokua, and Klebsiella pneumonia; and (3) Molecular identification of LAB by analysis of the base sequence of the 16S RNA gene. The whey for this study was collected in Lasi Farm in Agam Regency, West Sumatra, Indonesia. The data was subsequently subJected to descriptive analysis. The bacteria obtained were rod-shaped that were catalase negative and homofermen...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
Ali Wahdan1*, Mahmoud Ezzat1, Fatma Youssef2, Manal Munier2, Elsayyad M. Ahmed3, Marwa Abo Hashem1
...ity against varieties of antimicrobials. Three multi-drug resistant isolates carried ica from human abscess (A9), sputum of dog (A12), and cow mastitic milk (A13) were sequenced with other genome sequences retrieved from the GenBank. Ten samples (9.17%) were S. aureus from human, while 3 samples (1.24%) were S. aureus and 2 samples (0.83%) were coagulase negative staphylococcus (CONS) from animal sources. The multiple sequences alignments (MSA) and phylogeneti...
Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3
...is, and to determine the antimicrobial resistance. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical mastitis (SCM). Mastitic milk samples (n=541) were further used to investigate the prevalence of bacteria associated with mastitis and their antimicrobial susceptibilities by standard disc diffusion assay. The results showed that the prevalenc...
H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1
...norate, Egypt, and their antimicrobial resistance. A total number of 2160 samples included litter, pen litter, stored feed, feed from the feeders, water, drinkers, droppings, dust, swabs from walls, birds’ cloaca, worker’s hands, and wheels of vehicles. The results showed that there was a negative relationship between the prevalence of Enterobacteriaceae and the hygienic measures enforced in the poultry farms under study. The highest prevalence of ...
Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3
Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1*
...ectively. Data regarding antimicrobial susceptibility exhibited that among eight antibiotics E. coli, S. enteritidis, S. aureus and B. cereus were observed sensitive to gentamycin, norfloxacin and ciprofloxacin. K. pneumoniae showed sensitivity against gentamycin, norfloxacin, ciprofloxacin, erythromycin, tetracycline and streptomycin; whereas S. dysenteriae was observed sensitive to gentamycin, norfloxacin, tetracycline and ampicillin. In conclusion, raw beef...
Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti
...tes were also tested for antimicrobial and hemolysis activities. Five isolates from BSF larvae reared on chicken manure substrate and three isolates from BSF larvae reared on palm kernel substrate showed a gram-positive bacteria characteristic. The LAB isolates from BSF larvae reared on chicken manure and palm kernel meal substrate showed no different in inhibition zones against pathogenic bacteria (E. coli and S. typhimurium). All isolates showed no hemolysis...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
... in diabetic rats due to antimicrobial and anti-inflammatory activities. They can therefore be used as an effective remedy for healing wound infections in diabetics.
Keywords | Nanoparticle, Herbal plants, Diabetes, Staphylococcus aureus, Treatment
...
Fawziah M. Albarakaty*
...iseases. Finding a novel antimicrobial chemical with few side consequences is one of the most crucial phases in microbiological research because microbial resistance to chemical antimicrobial is the prevalent crisis in the therapeutic community. Objective: Therefore, this work aims to ascertain which phytochemical components can be recycled and added to the diet of animals while also examining the antibacterial, antifungal ...
Hira Jabbin1, Muhammad Arshad1*, Naunain Mehmood1, Wardah Hassan1, Syed Zakir Hussain Shah2 and Farzana Siddique3
...ural preservative having antimicrobial properties. In present study, the effectiveness of chitosan coatings as natural preservative was assessed on rancidity development and quality changes in mori fillets during 28 days of storage. The control and chitosan coated samples were analyzed periodically at intervals of 7 days, for determination of pH, water holding capacity, water extractable proteins, salt extractable proteins, thiobarbituric acid reactive substan...
Hams M.A. Mohamed1*, Katreen K.G.2, M.W. Abd Al-Azeem1, Faysal A. Wasel2, Ahmed M. Abd-Eldayem3
...pacity to form biofilms, antimicrobial resistance profile and virulence determinants, the purpose of this study is to discuss the presence of Listeria species in raw milk. In total, 150 samples of raw milk from vendors and nearby dairy farms in Sohag, Egypt, were collected. The samples underwent a bacteriological investigation. The results showed that 22 out of 150 samples had Listeria spp. contamination. Polymerase chain reaction (PCR) using the Listeria iap ...
Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2
...to applying eco-friendly antimicrobial finish on cotton fabric and checked mechanical property by pre and post-test and durability of this finish was checked up to 25 washes. The eco-friendly antimicrobial finish was extracted from leaves of Azadirachata indica, Butea monosperma and Litchi chinensis plants and applied on 100% cotton. Before and after applying antimicrobial finish mechanica...
Sayed H. Al Habty1, Dina N.Ali2*
...ogurt due to its highest antimicrobial activity and acceptable sensorial properties.
Keywords | MDR, Antibiotic resistance, Sensory evaluation, Yogurt, Ice cream, Damietta cheese
...
Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8
...loyed for evaluating the antimicrobial potential against 11 Gram positive bacteria, 9 Gram negative bacteria, 2 yeasts, 2 dermatophytes and 7 saprophytes. The petroleum ether seed extract and methanolic leaf extract was evaluated for the comparative anti-inflammatory and analgesic potential using different parameters like licking - biting response, inflammation of hind paw and writhing effect. The results showed that different pharmacological activities were d...
Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4
A. Yu. Aliev1*, A.A., Aliev1, A.M. Musaev1, M.Z. Magomedov2
...group, where the complex antimicrobial preparation Dioxinor was used, the effectiveness of treating serous mastitis in sheep was 96,3%....
Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5
...nclusion, CNE has better antimicrobial, pupicidal and adulticidal activity against C. pipiens than ordinary and binary mixtures with sesame oil.
Keywords | Culex pipiens, Nanoemulsion, Cinnamon, Sesame, Binary mixture
...
Noor Rahmawati, Alfi Rumidatul* and Sopandi Sunarya
...-negative For testing of antimicrobials, Escherichia coli and Pseudomonas aeruginosa were used. The experiment showed that the addition of yeast and sengon wood to PDB medium increased the growth and production of bioactive compounds extracted from fungal cultures. A fungal endophyte extract in ethyl acetate from all media used produced antioxidant compounds with high activity. Antibacterial activity was obtained from the extract of fungal cultures grown on PD...
Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7*
... well as improper use of antimicrobial therapy causes the treatment failure. Different types of antibiotics are used to treat it and different research use antibiotics of aminoglycoside group. For preventing the flock from infection separates the infected birds from healthy ones. Different treatments show average growth and good performance with the recovery of clinical signs, histopathological changes, mortality, and morbidity percentage. In this study, we re...
TAHIR MEHMOOD*, ANUM AFZAL, ABDUL KARIM, MUHAMMAD ASHRAF SHAHEEN &
HINA ARSHAD
... extracts exhibited good antimicrobial activity against Escherichia coli, Bacillus subtilus, Staphylococcus aureus and Salmonella typhimurium. Overall ripened lemon peel extracts showed higher antioxidant activity than unripe peels. The data presented in present study is an important factor to select ripened lemon peels as high potential values for nutraceutical, pharmaceutical and cosmetic industries.
Key Words: Citrus limon, Ex...
SYED ZULFIQAR ALI ABIDI, HAFIZ ABDULLAH SHAKIR, SYED SHAHID ALI, & JAVED IQBAL QAZI*
...ome of them are used for antimicrobial, fungicidal, herbicidal, piscicidal and molluscicidal. The present study was aimed to find out metabolites of piscicidal potential. Total thirty plants were collected on the basis of their availability and abundance around the year from different locations of Punjab, Pakistan. The aqueous and alcoholic extract of fresh and dried parts of each plant were used for qualitative estimation of thirteen metabolites (alkaloids, c...
SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM3
...s to use and develop the antimicrobial agents derived from some natural resources. Among various naturally occurring medicinal plants, Cinnamaldehyde, owing to its acid generation, acid tolerance and virulence gene expression has gained a considerable attention. Keeping in view its beneficial aspects, the present study has been conducted to investigate the contents and compare the nutritional and
Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*
...bited resistance to most antimicrobials classes. Meanwhile, the minimal inhibitory concentration (MIC) for amikacin and norfloxacin is significantly reduced from 64 µg/mL to 1 µg/mL and from 256 µg/mL to 8µg/mL respectively with frictional inhibitory concentration (FIC) index 0.25. Therefore, the FIC index recognized a synergistic activity between amikacin and norfloxacin against all P. areuginosa isolates. The mPCR was an efficient too...
Mansoor Ayoob1, Atta Hussain Shah1, Zaheer Ahmed Nizamani2, Muhammad Faisal Ayoob2,3*, Deepesh Kumar Bhuptani1,5 and Abdul Sattar Baloch4
...ucted to investigate the antimicrobial and antioxidant effects of honey marination on beef meat (M. longissimus dorsi). The samples were analyzed for moisture, fat, protein, glycogen, peroxide, thiobarbituric acid, total viable count and total coliform count after marination with 0, 10, 20, 30 and 100% concentrations of honey and after 1, 4, 8 and 12 days of storage (4 ̊C). The moisture, fat and protein contents were moderately decreased on the 4, 8 and 12th ...
Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*
... the correlation between antimicrobial resistance and virulence traits of bovine Streptococcal isolates. Induced resistance was conducted for Streptococcus pneumonia ATCC49619 and erythromycin-sensitive strains by gradually increasing the antimicrobial concentration. Plasmid conjugation test was carried out by membrane filtration method. The correlation between antimicrobial resistance and...
Meng Wang, Dongliang Zhou, Huixia Li, Chunqin Wu,Yan Zhao and Houqiang Luo*
...he target of alternative antimicrobial strategies based on small molecules and antibodies. This review mainly introduces the discovery history of the bacterial secretion system, structure, and function of T5SS, and summarizes its potential applications and existing issues. Presently we have not elucidated the specific pathogenic mechanism of this system, thus the intensive study is significant....
Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2
...ng of Campylobacter spp. antimicrobial resistance, selection and research of 2,120 samples of cecal contents from cattle, pigs, and poultry. The samples were analyzed by a microbiological method for the detection of Campylobacter according to the scheme of accumulation, isolation, isolation and identification according to DSTU ISO 10272-1:2007. As a result, 33 isolates of Campylobacter spp. were isolated, which is 1.6% of the total number of the researche...
Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1
...fectious pathogens, many antimicrobial and antifungal substances were previously found from synthetic and natural sources. Numerous investigations have demonstrated that the compounds coumarins, flavonoids, phenolics, alkaloids, terpenoids, tannins, essential oils, lectin, polypeptides, and polyacetylenes are present in medicinal plants. These bioactive chemicals serve as a foundation for the creation of antibiotics that are utilized to cure infectious illness...
Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova*
...arations were tested for antimicrobial (diffusion in agar by serial double dilutions) and antiradical activity (comparison with Butylhydroxyanisole (BHA), as well as for toxicity and the ability to cause irritation or an allergic reaction from the mucous membranes. In cows that used a suppository with a 40% content of C. leucocladum, the earliest recovery periods were revealed (6 ± 0.5 days), the number of cured animals was 80%. The use of intrauterine ...
Arshad Javaid1*, Iqra Haider Khan1, Malik F. H. Ferdosi2, Aneela Anwar3 and Mujahid Manzoor4
...nea possess antioxidant, antimicrobial, anticancer, anti-inflammatory, cytotoxicity, insecticidal and insect repellant properties as reported in the literature. ...
Tabark F Ahmed, Nibras Z. Yahya*
Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1
...plore the prevalence and antimicrobial susceptibility profile of bacterial organisms isolated from fish samples. A total of 120 fish samples were collected from fish retail market and ponds (60 from each source) comprising of three fish species i.e., Labeo rohita (LR; local name Rohu), Catla catla (CC; local name Thaila) and Cirrhinus mrigala (CM; local name Morakhi). The samples were processed for isolation of bacterial organisms using conventional culture me...
Eman M. Abdelhady1, Nader Y. Mostafa1, Mohammed A. El-Magd2* and Ghada A. Kirrella1
...o treatments. With these antimicrobial and antioxidant properties, CP and CNPs, with a better effect for CNPs, could be used as preservatives for the minced meat to extend its shelf life....
Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*
...antly reduces the use of antimicrobial agents. This study aimed at preparing and evaluating inactivated polyvalent mastitis vaccines containing bovine origin Staphylococcus aureus (tst), Streptococcus uberis (cpn-60 targeted STUB), and Escherichia coli (aggR) in a rabbit model. Four types of mastitis vaccines viz. formalin-inactivated plain polyvalent mastitis vaccine (FPPV), formalin-inactivated montanide adjuvanted polyvalent mastitis vaccine (FMPV), formali...
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
...xtracts showed promising antimicrobial activity
in terms of zone of inhibition against selected bacterial strains, both Gram
positive and Gram negative i.e. from 1.33 mm to 25.66 mm and against
fungal strains i.e. from 1.33 mm to 24.33 mm. These findings suggest that
the different parts of R. laetus exhibiting different biological activities and
could be used as a promising source of novel drugs.
MALIK ASIF HUSSAIN
...film communities against antimicrobials.
The layers of biofilm provide a protective cover around bacteria and
hinder penetration of these agents deep. Furthermore, this tolerance also
helps them to survive against immune system, as immune system cells
and other components cannot penetrate through layers of biofilm. This
review paper discusses important aspects of biofilm formation, clinical
Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1
...
...
...
Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3
Waseef Ullah Khan*, Yasser Durrani and Muhammad Ayub
... natural antioxidant and antimicrobial preservative for refrigerated mango OLE blend....
Muhammad Shahid Nisar1*, Ahmad Kamran Khan1, Ghulam Fatima2, Irfan Ahmed4, Khizar Sami Ullah2 and Syeda Amber Hameed2,5
...s, natural antioxidants, antimicrobial compounds and substantially reduce the amount of waste....
Yunxia Guo1, Xia Zhou1*, Xin Huang2 and Fagang Zhong2
...applied to calculate the antimicrobial resistance profile of bacterial isolates. The survey results showed that bronchitis and bronchopneumonia were commonly occurring respiratory diseases in cattle. The highest incidence of bacterial organism Staphylococcus aureus (83.6%), followed by Streptococcus pneumoniae (80.3%) and Klebsiella pneumoniae (77.0%) were recorded in the respiratory tract samples. Pasteurella multocida, Bacillus obstructivus and Mycoplasma al...
Frah R Kbyeh1*, Ahmed N. Abedsalih2
...aimed to demonstrate the antimicrobial effect of diclofenac, Ciprofloxacin, and a combination (diclofenac +ciprofloxacin) against clinical resistance E coli o157 H7 isolates by using Polymerase chain reaction (PCR). The presented study, all rabbits, forty-eighth adult female rabbit aged (6-8 weeks) and weighing between (1500 and 2000) gm., was randomly divided into six equal groups(8/each) groups injection 0.1 ml in 1 x 109 CFU Escherichia col...
Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7
... was carried out for the antimicrobial potential of leaves Tulsi, Curry and Ashoka, targeting two Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) as well as two Gram-negative bacteria (Vibrio parahaemolyticus and Escherichia coli) and fungi viz., Aspergillus niger, Alternaria alternate, Fusarium solani and Aspergillus flavus were studied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,1...
Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1*
...Caralluma possesses high antimicrobial activity against E. coli. and Bacillus causing 16mm±0.53 and 12.6±0.43 while ethyl acetate solvent of plant extract against E. coli. and Bacillus inhibited 08±0.0.03 and 9.6±0.32mm respectively. Ethyl acetate extract showed the best inhibition against Pseudomonas and Staphylococcus 15.6±0.54 and 9.8±0.41mm respectively and the same extract exhibited the zone inhibition 0.68±...
Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1
... antibiotics and as food antimicrobial agents to potentially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population on chicken breast cuts in the form of a phage cocktail. Out of 17 isolated bacteriophages, three bacteriophages (SEPL01, SEPL13, SEPL20) exhibited maximum lytic po...
Tarek Refaay, E.A. Elshafiee, Hayam A. Mansour and Maha A. Sabry*
Haseeb Zafar1, Asghar Ali Kamboh1*, Zain-Ul- Abideen1, Muhammad Kamran Taj2, Sibtain Ahmad3 , Umbreen Zafar2
...li isolates to different antimicrobial agents. A total of 150 samples from a variety of sources (cecum, feed, bedding, water, air, and flies; n = 25 each) were collected from five distinct broiler farms (n=30/farm) in the district of Quetta. Samples were streaked on selective (Xylose Lysine Deoxycholate agar) and differential medium (MacConkey’s agar) plates and incubated at 37°C for 24 hours. After isolation, organisms were further identified throug...
Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati
...g global significance of antimicrobial resistance (AMR) genes within diverse microbiota components has garnered increasing attention due to its profound consequences. Recent investigations have revealed the presence of antibiotic-resistant bacteria among wild bird species in remote habitats, suggesting the potential for these avian populations to serve as reservoirs and sources for emergent AMR profiles. Recognizing the critical role of wildlife in AMR dynamic...
Shanti Fitriani*, Usman Pato, Yusmarini Yusuf, Emma Riftyan, Evy Rossi
SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2
...ays an important role in antimicrobial action. The current study was aimed to assess of antibacterial activity of ethanolic, methanolic and aqueous extracts of Aloe vera L. against six bacterial strains (Staphylococcus aureus; Streptococcus pyogenes; Shigella sonnei; Escherichia coli (1) (4C11); Escherichia coli (2) (ATCC 13048) and Neisseria gonorrhoeae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their anti...
IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3
...h aimed at assessing the antimicrobial properties of Dalbergia, Brassica and Trifolium honey samples against microorganisms isolated from infected burned skin of patients in children hospital, Lahore, Pakistan. The isolated microorganisms were identified as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were...
SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1
...erial associated UTI and antimicrobial susceptibility pattern was determined by Kirby Bauer’s disc diffusion method. Samples (n=85) were analyzed to determine the bacterial strains involved in infectious cases and relationship with age, gender and social status of patients was determined. Results of the study revealed that 61% of samples were positive for Escherichia coli, other contributing pathogens were Staphylococcus spp. (24%), Klebsiella spp. (9%),...
HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
...ext-align: justify;">New antimicrobials agents are needed to combat drugs resistance in microorganisms. The aqueous and methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of ext...
ZUBIA RASHID1, ZULFIQAR ALI MIRANI2, SITWAT ZEHRA1, SYED MUDDASSAR HUSSAIN GILANI3, ARIF ALI CHISHTI1, ABID AZHAR1 & SADDIA GALANI1*
...ry, development of novel antimicrobial strategies for drug-resistant pathogens is desired. Here, Phytogenic Feed Additives (PFA) were administered in chicken diet to decipher their impact on animal performance and gut microbiota composition. One-hundred-fifty-day-old chicks were randomly divided into three treatment groups with five replicates/group as (i) Control diets (CON); (ii) Control diets + 0.01% w/v enramycin (antibiotic growth promoter, AB); (iii) Con...
SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3
...nts in effective and neo-antimicrobials research, one wonders why not a single infectious agent has been completely knocked out. Our group has been focusing on ascertaining the basis of antibiotic deferring processes acquired by (indigenous) clinical strains. Accordingly, sub-lethal doses of the drug result in development of hyper-resistances. The bacteria have evolved the molecular genetical basis (and other parameters) for acquiring the resistance. For the c...
Teedzai Chitura*
... the main contributor of antimicrobial resistance. As a result, there have been growing calls by consumers and international health organizations for reduced application of antibiotic growth promoters in livestock production. To address this problem, several animal feed commissioners and summits have legislated and banned the use of antibiotic growth promoters in livestock feeds. Given these scenarios, animal nutritionists embarked on the quest for the search ...
Muhammad Atif Raza1, Muhammad Tariq Javed2, Muhammad Fiaz1*, Muhammad Shakeel3, Muhammad Shahbaz Ul Haq2, Amna Kanwal2, Syeda Maryam Hussain1 and Muhammad Zubair Siddiqi4
...ding to the induction of antimicrobial resistance in microorganism. Main aim of this study was to find out substitute of antibiotic against Fowl Typhoid in broiler in terms of immunological, serum biochemistry and lipid profile parameters. Broiler chicks (Age = 1 d and n = 90) were kept under uniform management conditions. Chicks were divided randomly into six groups on day ten of their age with 15 replicates in each; control negative (CN), control positive (C...
