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Qin Zhao1,2, Yani Sun1,2, Gaiping Zhang1,3, Frederik Widen4, En-Min Zhou1,2,*

zhouem@nwsuaf.edu.cn

...plication in serological diagnosis of HEV.

...

James Ayukepi Ayukekbong1,2

E-mail | james.ayukekbong@microbio.gu.se

...reas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to initiate the appropriate clinical care. Despite the endemicity of dengue in Nigeria, the disease is not a reportable disease and routine diagnosis is ...

Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4

... minute. Taken together, diagnosis based on five minute standing using the Romberg test appear to be sensitive and accurate than one minute.

...

Moustafa Kardjadj

...nerated in epidemiology, diagnosis and surveillance of the disease in the Maghreb countries.

...

 Zahir Shah, Shakoor Ahamd, Mian Saeed Sarwar, Murad Ali Khan and Javid Ali

...ilical region. Tentative diagnosis was made through clinical sign and physical examination; the area was incised under local anesthesia. After surgical procedure site was closed by applying mattress sutures. Adjunct therapy of broad-spectrum antibiotic was administered intramuscularly for five days as a post-operative measure. Catamnesis revealed that the healing was complete in 14 days with no recurrence and untoward consequences.

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Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A

...y PCR. This confirms the diagnosis of concurrent infection of CIA and IBD in the affected flock. This is the first case report of a concurrent and natural field outbreak of CIA and IBD in a commercial poultry farm in Nigeria.

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Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...ign: justify;">Effective diagnosis and control measures for Newcastle disease virus (NDV) requires continuous disease surveillance and understanding of field NDV strains. The NDVs, a prototype of Avian Paramyxoviruses (APMV-1), have been reported previously both in chicken and feral birds (natural reservoirs) in Pakistan; however, their molecular characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular ...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...used as complementary in diagnosis of amphistome infections in ruminants. 
...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...A) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, histopathological examinations and PCR analysis. The results showed, 20 chickens as positive for ALV-A (2.73%, 95% CI 1.7-4.2) and these chickens were found to be positive for ALV-A with the further distribution of 2.91% (95% CI 1.0-6.7), 3.70% (95% CI 1.5-7.5), 2.48% (95% CI 0.5-7.1), 3.13% (95% CI 0.6-8.9) and 1.30% (95% CI 0.2-4.6) in Anhui pro...

Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*

...l for early and accurate diagnosis of Mycoplasma infections from samples collected from clinically sick and apparently healthy birds. In this way, current approach could be contributed well towards elimination and reduction of losses due to Mycoplasma infections in commercial poultry.

...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
... have revolutionized the diagnosis of plant viruses. While the technologies available to virologists have obviously become more diverse and improved, the challenges have also changed greatly.Detection of plant viruses is becoming more critical as globalization of trade,particularly in horticultural commodities increase. The potential effects of climate change have further aggravated the movement of viruses and their vectors, transforming the diagnostic landsca...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
...ided a precise molecular diagnosis. Theidentification of the same variant in CTSK as identified in other families from Pakistan suggests that it is common due to a founder effect.
...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
... has its pivotal role in diagnosis, prognosis and comparative studies. c-Myc gene was selected to study the mutation and expression profiling of different canine and feline tumors due to its predetermined role in tumor pathogenesis. A total of 52 tumor and normal samples were examined, among these, 40 canine and 12 feline samples were analyzed using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The ...

H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2 

...stic procedures used for diagnosis of bovine brucellosis on serological, bacteriological and molecular basis. A total of 141 cows from brucella infected farms under quarantine of the veterinary authorities were employed. Serological examination using BPAT, RBT and CFT in 141 cows revealed 109 (77. 3), 105 (74.47) and 104 (73.76) seropositive respectively. Relative sensitivity, relative specificity, positive predictive value and negative predictive value of BPA...

Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2

 

...est is available for its diagnosis. It is considered one of the most costlydisease in terms of diagnosis and treatment. Its heritability content is considered to be up to 80% but still it isunknown whether it’s a pure genetic disease. It is considered a polygenic multifactorial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizophre...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
... serological methods for diagnosis, we found a 24.13% (168/696) overall seroprevalence, where the seroprevalence for H3N8 was 14.51% (101/696), for H7N7 6.03% (42/696), and 3.59% (25/696) as mixed infections, suggesting EIV current and active circulation in equine population of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in ponds and rivers”, and “geogr...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...o. So rapid and accurate diagnosis of FMD is uttermost need of this time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known ser...

Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar

... was conducted to make a diagnosis of endometriosis. Females were divided into two groups. Females in group A received treatment with Letrozole tablets (2.5 mg/day) and in group B received Danazol tablets (600 mg/day) for 3 months. Pelvic pain was assessed using VAS score and the mean decrease in pain score was assessed between two groups using independent sample student t-test. Results of this study demonstrate that the mean age of the patients was 29.99&plus...
Wazha Mugabe1,3,Gaolebale Segolame Mpapho1, John Kamau1, Wameotsile Mahabile2, Shalaulani James Nsoso1, Kealeboga Dipheko1 and Assar Ali Shah3,*
...agnose the disease. Most diagnosis was based on clinical observations (68) with the low utility of California Mastitis Test (CMT) (3%). Milk production was at subsistence scale and a secondary product from meat type breeds. With a commercial mindset, improved goat management and cooperative pooling and marketing of milk the current farming setup of using meat breed for milk could prove beneficial to both the meat and dairy sectors.
...
Sadaf Noveen1*, Shaista Habib Ullah2, Babar Alam3
... important for etiologic diagnosis and treatment planning among children without any disability or ignored craniofacial deformities. Studies highlighting this relationship are rare to none in Pakistan. Therefore, this study aims to detect the type and extent of relationship between these two factors.
Methods: A descriptive cross sectional study was conducted on a sample of 140, which were 4-8 years old children with speech sound disorders, w...
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
...which facilitates prompt diagnosis of multidrug resistant TB. These results propose that effective treatment can be initiated at an early stage, which will greatly help in reducing multidrug resistant TB.
...
Matiyar Rahaman Khan1,*, Sabayasachi Pal2, Amit Singh2, Ashok Dhananjaybhai Patel3, Bhagubhai Ambaram Patel3Tushar Manohar Ghule2 and Victor Phani1
... for its easy detection, diagnosis, dimension of damages on citrus, and Bt-cotton under Indian conditions.
...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
...case report presents the diagnosis and screening of suspected rabies samples of cow and mule. Clear behavioral changes along with paralysis of tail and hind legs were noticed in the mice of both groups between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT). Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying th...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
... techniques used for the diagnosis of MCD were visual Acuity test by Snellen chart, phoropter, slit lamp biomicroscopy, topography, keratometer and pachymetry. In this study, 50 patients of MCD were identified among which 40 were males and 10 were females, including 9 cases with family history. Main complaint was drop in visual acuity and loss of vision in all patients (100%) due to damage and accumulation ofkeratan sulfate (KS) in macular layer of corneal str...
Asima Bano1, Hafiz Muhammad Tahir2, Hira Sherawat3, Muhammad Mutlib4, Muhammad Arshad5, Muhammad Akram Qazi6, Sajida Naseem5, Rabia Ishaq1, Iram Liaqat2 
...used as biomarker in the diagnosis of breast cancer. 
...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
...olled in this study. The diagnosis of canine visceral leishmaniasis was performed by the immune fluorescence antibody test with antibody titter ≥1:128 and lymph node smear examination. In blood samples of dogs with visceral leishmaniasis, levels of malondialdehyde, total antioxidant capacity, glutathione, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines (TNF-alpha, IL-1β) were determined and compared with the healthy controls. Significa...

Riffat Mehboob1*, Sami Ullah Mumtaz2, Zoya Manzoor3, Sajid Abaidullah2, Fridoon Jawad Ahmad1

Deranged biochemical and hematological profile of septicemia patients in Mayo hospital, Lahore
... kidney and blood in the diagnosis of septicemia. 101 confirmed patients of septicemia from a tertiary care hospital in Lahore were included. Liver and renal function tests were performed for all patients. Patients were divided into 3 age groups on the basis of age: 30-50, 51-70 and 71-90 years. In Liver function Tests, ALT (37.62%), AST (50.49%) and ALP (99%) were elevated, Bilirubin was normal in majority of patients while total protein was in normal range i...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*

Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3 

...SA and Real-time PCR for diagnosis of Theileria parva at Veterinary Research Institute Peshawar. The overall prevalence for Theileriosis was 27.75% through Real-time PCR, followed by Indirect ELISA (24.62%) and Microscopy (11.37%). Transect wise high prevalence for Theileria parva was observed in Lakki Marwat with 33.5% for Real-time PCR, significantly different at P<0.05 from Peshawar. Cattle showed 100% more positive results as compared to Buffalo. All th...
Nasira Khatoon1, Aly Khan2,*, S.S. Shahid3, Z.S. Saify4, M. Arshad Azmi1 and Wali Khan5
... at the first visit. The diagnosis was based on detailed history and clinical examination which included scraping of papillae or burrow to confirm the diagnosis. To test the incidence in various age groups and body parts a chi-square (χ²) test of association (R × C contingency test) was performed. The association of body parts and age groups was significant. Most of the patients above the age of 10 were...
Rishen Liang*, Meng Zhou, Zhenxiang Lin, Guozhang Li, Yuan Chen, Xuan Lin and Zaohe Wu
...the threshold of species diagnosis divergence value of 2%. Inner individual divergence values of each species were less than 0.5%. In the molecular phylogenetic trees, the longer branch length also clearly distinguished the independent placement of the two species. These results revealed great genetic differences between P. orientalis and P. vittatus, and strongly suggested that they might be two distinct species and should not be placed as synon...
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...sup>th day and the diagnosis of EM were not individually reliable in every animal. However, it is thought that in between 28th day and 40th day, statistically significant difference (P<0.01; P <0.001 between the pregnant and EM animals can be predicted.
...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...analysis was used to get diagnosis outcome of selected miRNA panel. Upregulation of miRNA panel (mir-376c, mir-155, mir-17a and mir-10b) has been seen in the plasma samples of TNBC patient´s samples relative to non-TNBC control samples. The scatter plots and other statistical analysis have given differences in expression of four miRNA panel with significant p-value (p< 0.001), respectively. Kruskal Wallis test and Mann Whitney U test have clearly show...

Muhammad Rehan1, Asim Aslam1, Muti-ur-Rehman Khan1, Muhammad Abid2, Shakeel Hussain3, Javeria Umber4, Ahsan Anjum1 and Altaf Hussain2* 

...rains is limiting in its diagnosis and control. Pakistan is a developing country and poultry industry is the backbone of its economy. Recent outbreaks have caused huge losses to the poultry industry. This review details the possible role and potential of some common captive and non-captive wild bird species in the spread of NDV and also its economic impact on the commercial poultry industry of Pakistan.  

...

Mehboob Arslan* and Xin Yang 

... in order to improve the diagnosis and drug development of future antiviral therapeutics. In fact, metabolism is considered as a crucial player in the cascade of viral interactions, its modulation is essential to decide the final outcome of viral infection. Here, we concisely present how viruses alter the metabolic pathways for their own survival.  

...

Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3 

Shaukat Ali1,*, Sadia Batool2, Fatima Javed Butt2, Sundas Nasreen1, Hafiz Muhammad Tahir1
...e treatment strategy for diagnosis and treatment of cancer; it is a second chief cause of deaths in the world. Advances in chemotherapeutic agents, various radiological techniques and bio-nanoparticles treatments (i.e., green synthesized metallic nanoparticles) attracted many researchers to conclude a novel cancer treatment strategy. Unluckily every strategy had some working limitations as a result of which cancer remained a catastrophe for humanity. The prese...

Ankur Abrol, Madhumeet Singh, Akshay Sharma* and Pravesh Kumar 

...he correct and efficient diagnosis which can mainly be achieved by anamnesis, vaginal and rectal examination. However, prognosis of uterine torsion is mainly dependent on the degree and duration of condition and can be categorised into 3 successive stages i.e. stage of positive prognosis, stage of less positive prognosis and stage of negative prognosis. Body condition assessment of the dam is of paramount importance as it can provide a snapshot about the possi...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...ultry.
...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*
...div>...
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
...oup that may allow prior diagnosis and selection of effectual antifungal agents.
...

Ihsanullah Kakr1, Sarwar Khan2, Khalid Khan3* and Sajjad Ahmed

...e experienced in disease diagnosis and use of traditional veterinary practices. 

...

Shilong Chen1,2, Shifeng Xiao1,2, Shao Wang1,2, Fengqiang Lin1,2, Xiaoxia Cheng1,2, Xiaoli Zhu1,2, Dandan Jiang1,2, Shaoying Chen1,2* and Fusong Yu3* 

...cal symptoms, laboratory diagnosis, genomic organization and pathogenesis. However, the pathogenesis mechanisms of cross-species transmission, retardation and beak atrophy are still needed to be elucidated. 

...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found to be infected with malaria parasite. Data was also collected sfrom other labs of District Swat which accumulated to a total of 9255 patients, among which 932 (10.07%) patients were found positive for malaria parasite. Male infected were 558 (59.87%...
Muhammad Tariq Rasheed1, Imran Bodlah1*, Ammara Gull e Fareen1,2, Muhammad Shakeel Khokhar1
...f Pakistan. Differential diagnosis, morphometric and illustrations are provided with notes on distributional range.
 

Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2 

...e epidemic. However, the diagnosis of HCV infection is faced with several challenges, therefore there is a demand for development of easy to use and inexpensive molecular methods for accurate diagnosis. The sub-Saharan Africa HCV genotypes distribution are diverse, but genotype I is the most common. This review highlights the need for more robust surveillance studies with introduction of opt-in testing at all clinics for bet...

Muhammad Masood Tahir1, Ayyaz Hussain2, Saeed Badshah3, Qaisar Javaid2 

RULE-BASED IDENTIFICATION OF BEARING FAULTS USING CENTRAL TENDENCY OF TIME DOMAIN FEATURES
...g classifier-based fault diagnosis, even if the vibration signals are
contaminated with considerable background noise. 

...

Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*

Hepatitis E Virus and Zoonosis
...mented. The conventional diagnosis is established by detection of HEV antigen or anti-HEV IgM in acute sera. However, the detection of HEV RNA is important to establish the diagnosis in immunosuppressed patients. In transplant patients, decrease in immunosuppression is usually done, however ribavirin therapy has also shown a definitive role. There is upcoming literature on the role of sofosbuvir also in these groups of patie...
Muhammad Zahid Mengal1, Hamida Ali2, Raheela Asmat3, Muhammad Naeem1,4, Ferhat Abbas1, Abdul Samad1, Mohammad Zahid Mustafa1, Jannat Raza2 and Tauseef M. Asmat1*
...ionized the tuberculosis diagnosis by simultaneous detection of Mycobacterium tuberculosis and resistance to RIF (rifampicin), a surrogate marker for multidrug-resistant TB in less than two hours. The RIF-resistance pattern in Balochistan, Pakistan, is not documented. This study was aimed to detect RIF-resistant TB and mutations in RNA polymerase beta (rpoB) gene of M. tuberculosis within 81-bp RRDR in Quetta, Pakistan using GeneXpert...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...to H9N2 infection, rapid diagnosis is an option to prevent further infections. Office International des Epizooties (OIE) suggested some methods for the detection of H9N2 infection including the immunofluorescence method that is commonly known as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity is influenced by antibody production methods. Therefore, t...

Zafar Ahmad Handoo1*, Mihail Radu Kantor1 and Ekramullah Khan2

Description of Seven New Species and One New Record of Plant-Parasitic Nematodes (Nematoda: Tylenchida) Associated with Economically Important Crops of Kashmir Valley, Jammu and Kashmir (Part-1 of the series)
...iption, characteristics, diagnosis and relationships of each new species with its closely related species are given.

...
Faryal Saad1,3, Zia Ur Rahman2, Aamir Khan3, Rabia Noushin4, Irfan Ullah5, Farman Ullah Dawar3,Shahid Naiz Khan3, Rafiqe Hussain6, Kenza Javed2Abid ur Rehman7, Amna Fayaz3, Zohra Saifullah3 and Kalim Ullah3*
...ectively. Proper in-time diagnosis of CMV is the most effective way to control this disease. Lack of health care facilities and proper screening of pregnant women for various congenital infections in the Sothern districts of Khyber Pakhtunkhwa, making the situation more worst. Therefore, this preliminary study was set to assess the seroprevalence and molecular detection of CMV infection among pregnant women of districts Lakki Marwat and Bannu of Khyber Pakhtun...

Sidra Shehzadi, Sher Bahadar Khan*, Umar Sadique and Saqib Nawaz

Isolation and Molecular Identification of Clostridium perfringens Type D in Goats in District Peshawar
...ens Type D for effective diagnosis, treatment and vaccination. A total of 100 fecal samples  were collected aseptically from four different zones of district Peshawar during the period of February, 2019 to April, 2019. C. perfringens  Type D was confirmed through bacterial culturing, Gram staining, biochemical tests and polymerase chain reaction (PCR) . Results revealed that 25 feacal samples collected from suspected goats were positive for C. perfri...
Muhammad Riaz1*, Zahida Tasawar1, Muhammad Zaka Ullah2 and Zawar Hussain3
Parasitological and Molecular Survey on Theileriosis of Sheep and Goats and the Related Risk Factors in Musa Pak Shaheed Town, Multan, Pakistan
... and sensitive assay for diagnosis of ovine and caprine theileriosis.

...
Mohsin Ali1, Nargis Haider Kakar2, Rida Dawood3,4, Qurat-ul-ain Fatima5, Zunera Tanveer2, Shoaib Ahmad Malik6, Tauseef Muhammad Asmat1, Asadullah1, Muhammad Masood Tariq Kiani1 and Mohammad Zahid Mustafa1*
...s recommended for timely diagnosis of thyroid abnormalities in diabetics to lessen the risk of development associated co-morbidities.
...
Abdul Hafeez1*, Akhtar Nawaz Khan2 and Zahid Ullah3
Faiz Muhammad Khand1,Allah Bux Kachiwal2, Zubair Ahmed Laghari2, Shakeel Ahmed Lakho1, Pershotam Khattri2, Saeed Ahmed Soomro2, Nazar Ali Korejo2 and Ambreen Leghari1*
...arliest day of pregnancy diagnosis was 19 day after mating, while trunk was best parameter for determination of gestational age throughout gestational period and fetal sexing can be diagnosed at 7th week of gestation period in teddy goats.
...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...who were recommended for diagnosis of malaria. About 1050 patients were examined and malaria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found infected with malaria parasite. Comparative Data was also collected from other labs of District Swat which accumulated to a total of 9255 patients, among which 932 (10.07%) patients were found positive for malaria parasite. Male infected were 558 (5...
Ahsan Numan1,4, Abdul Basit Qureshi2, Fehmeda Farrukh Khan3,Faisal Masud3,Ijaz Ahmad4, Kamran Ashraf5, Nisar Ahmad5, Muhammad Shahbaz Yousaf 4, Imtiaz Rabbani4, Hafsa Zaneb6 and Habib Rehman4*
...uropathy (DSP). Clinical diagnosis of DSP was assessed with the Michigan Neuropathy Screening Instrument. The diagnosed patients with DMT2 were randomly distributed into 2 groups. Group-I (n= 79) included the patients with DSP having <10 years of duration of DMT2, called short duration, while DSP patients of Group-II (n= 49) had duration of DMT2 for ≥10 years, thereafter called long duration. The patients in both groups were compared with their correspon...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

... BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections which leads to serious complication which may be fatal in young calves up to 3 months of age. A total of 20 fecal samples were collected in the winter season of 2018 and 2019, all samples were serologically screening by antigen-capture ELISA, then molecular confirmation by RT-PCR targeted to nucleocapsid protein ...
Fuhua Zhang, Yishuang Yu and Shibao Wu*
...-size: small;">Pregnancy diagnosis is critical to the management of captive breeding of wild animals, as accurate pregnancy diagnosis can improve the reproductive efficiency of captive animals. Pangolins do not show obvious morphological changes during pregnancy, which increases the difficulty of pregnancy diagnosis. In this study, pregnancies were diagnosed using B-ultrasonography in thre...
Maria Qibtia1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Sehrish Faryal1, Ahmed Mansouri2, Zeeshan Ahmad2, Muhammad Hamid3 and Ali Raza Awan1,*
...g of the pre-symptomatic diagnosis of LNP. Among the six novel mutations found, mutation XI was found in all of the LNP subjects and was absent in the lactose persistent group. This study, for the first time, focuses on molecular analysis of LCT-gene from Pakistani patients with LNP.
...

Mohammed Bashar Danlami1*, Basiru Aliyu1, Abbas Bazata Yusuf1, Bashar Gulumbe Haruna1 and Asmau Nono Mahe1

...he hospital for possible diagnosis of cervical cancer. Descriptive and summary statistics and cross-tabulation were used to describe the data using the Statistical Package for Social Sciences. Out of the 92 women who had cervical cytology by Pap smear during the period under review, 22 had abnormal pap smears, a prevalence rate of (23.9%); 7/92 (7.6%) were low-grade squamous intraepithelial lesion while 12/92 (13.0%) were high-grade squamous intraepithelial le...

Mokbel, Samah A.1,Ahmed K. El Attar1; Azza G.Farag2.

...uencing was used for the diagnosis of the
witches‟ Broom infection. Total DNA was isolated from leaf tissues of infected hibiscus
plants. Nestedpolymerase chain reaction(PCR) was performed using the universal -
phytoplasma specific primers; P1/P7, R16F2n/R16R2.Witches‟ broom specific primers
SR1/ SR2, at the spacer region (SR), were used for the direct PCR. Amplicons of
expected size characteristi...

Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy

...wide. Rapid and accurate diagnosis of RV infection is crucial for appropriate patient management and infection control. The aim of our study was to compare the diagnostic performance of Lateral flow immuno-chromatography assay (LFICA) as a rapid test for detection of RV antigen in stool specimens collected from Egyptian infants and young children with ELISA, and nested reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequenc...

Hanaa A. Ahmed1 and Eman M. AboHatab2

Nashwa M. Helmy1 and Ahmed S. A.2

....
Definitive diagnosis of foot and mouth disease requires the detection of virus antigen or
genome. So, the vesicular epithelial tissues collected from infected cattle from Sharkia
(7) and Fayoum (5) governorates were subjected to antigen ELISA and RT-PCR. 8
epithelial tissues were identified by Indirect sandwich ELISA. The results were 5 and 3
positive from Sharkia and Fayoum respectively, while 12 s...

Amira A. Mazyad1; A. A. Kheder1; Ahmed K. El-Attar1; W. Amer.2; Mahasen, H. Ismail.2; Amal, A.F.1

...at room temperature. The diagnosis of SLRSV in the infected tissues of strawberry plants was performed using both serological (DAS-ELISA) and molecular assays. Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify 497 bp fragment using PCR primers specific for the viral coat protein gene as a tool for molecular diagnosis. The PCR detection was confirmed with direct DNA sequencing and phylogenetic analy...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

...n the identification and diagnosis of bovine ephemeral fever disease revealed that the virus isolation is a gold standard technique, as well, the immunofluorescence technique. RT-PCR proved to be a rapid, sensitive and specific tool for bovine ephemeral fever disease diagnosis.

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Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

...R. While results of sero-diagnosis showed that 45/100 from apparently healthy non vaccinated cattle and 68/100 from vaccinated cattle were positive by SNT respectively and in general 90/200 of tested cattle give protective antibody titer, while 23/200 gave non protective titer and 87/200 have no antibody againest LSDV. The results of clinco-pathological revealed highly significant increase in ALT, AST, ALP, GGT, urea and uric acid, while the level of total pro...

Aly M. Abdel-Salam

...severe problems in virus diagnosis. Objectives: The purpose of this study is to characterize biologically, serologically, and molecularly one Crinivirus viz. Cucurbit yellow stunting disorder virus (CYSDV) and one Ipomovirus viz. Cucumber vein yellowing virus (CVYV) from infected cucurbits. Further, the study is concerned with illustrating the adverse effect of mixed infection with these viruses on diagnosis and breeding for...

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

...ive: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of NDV was amplified using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) then subjected for nucleotide and amino acid sequence detecti...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

... golden standard for IBV diagnosis so the
aim of this study was comparing the different biological systems for isolation of infectious bronchitis
virus.
Methods: The Egyptian variant 2 IBV strain representative to the current circulating strain in Egypt
was isolated in specific pathogen free (SPF) embryonated chicken eggs ECE), chicken embryo kidney
cell (CEK) and in Vero cell line and was confirmed f...

Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3

...smission and serological diagnosis, in addition to the ultrastructural changes produced in potato
leaf cells infected with AMV .
Results: Reaction of thirteen plant species and cultivars belonging to four families (Amaranthaceae,
Solanaceae, Fabaceae and Laminaceae) to AMV infection was demonstrated. The presence or
absence of the virus was verified by back inoculation onto healthy indicator host plant and/or ELI...

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

... the rapid and sensitive diagnosis of FMD in epithelial samples.
...
Syed Maaz Nadeem1,*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh2, Arfan Ahmad2, Syed Nadeem Mansoor3 and Muhammad Anees4
... approach for supportive diagnosis of chicken anemia virus infection and therefore could be replicated for efficient disease diagnostics in resource-limited setting.
...
Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4
...issed
in the diagnosis because it is symptomless and its lesions can regress by time. On the other hand there
is no cell culture for Human papilloma viruses (HPVs).
Objective: Detection of HPV genotypes assocciated with FEH in childern aged three to fourteen years
old.
Methods: Fourty seven biopsies and oral smears were collected from Children (3 -14) years old, who
attended the pediatric ...

Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2

...ron microscope for rapid diagnosis, host reactions, serological
double-antibody sandwich (DAS)-ELISA, reverse transcription (RT)-PCR and nucleotide
sequence determination. Ultra-structural responses of basil leaf cells infected with a
morphologically distinct RNA virus, AMV, were studied. Molecular characterization and
phylogenetic analysis were performed for the AMV coat protein (CP) gene. An amplicon of the

Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2

...ed bio-security. Regular diagnosis and viral screening will be helpful in the eradication of the new strains to predict the newly coming waves of infection and to be able to develop the recommended vaccines. Objective: Our study aimed for Molecular Diagnosis of Avian influenza virus (AIV) from Different Avian Species that would be useful to detect the current strains present in Egypt to use it in vaccination program. Methods...

Fahima Khatun1, Abdullah-Al-Maruf2, Md. Mizanur Rahman2, Afroja Yasmin1, Mohammad Ali Zinnah3, Md. Aminul Islam4 and Mohammad Shah Alam5*

...t the clinicians for the diagnosis of such parasitic infections and necessary steps for prevention and control measures against them.

...
Mona Mohammed EL-Derbawy1, Eman Naser Hafez2*, 
Mona Abdel Daym Abd Rabbo1, Nagwa Ibrahim Toaleb3,
 Saedia Abdel Hady Sayed El-Ahl1 and Bahaa Eldkeen Wade EL-Aswad4 
...s (SCA) by ELISA for serodiagnosis. Total IgG ELISA was assessed to evaluate the potency of two parts of Schistosoma mansoni ceracrial antigens (2000 each) one normal and other exposed to attenuating dose of 400 Gy gamma radiations. The study was conducted on fifty two individual sera samples divided as follows: Group І included 21 obtained from patients who were positive for schistosomiasis and negative for other parasitic infections; Group ІІ inclu...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
...be used as reference for diagnosis of C. parvum infection.
...
Shoaib ur Rehman1, Jabbar Khan2*, Raaza Malja Khan3, Maimoona Azam4 and Zeeshan Mutahir5
...prevention like prenatal diagnosis (PND), genetic counseling and carrier screening for controlling the affected births in the population. Consanguinity, lack of awareness and non-availability of health facilities have thus contributed to the high occurrence of β-thal and miserable life of the patients.
...

Md. Sanjid Hasan1, A K M Humayun Kober1, Eaftekhar Ahmed Rana2, Md Saiful Bari1,3,4* 

... (CMT) were used for the diagnosis of SCM. Milk samples from CMT positive cows and swab samples from cows’ udder surface with lesions were collected for laboratory analysis. Laboratory analysis including bacterial culture and biochemical tests were done to identify the causal agents of SCM and udder lesions. The overall prevalence of SCM and udder lesions were 56.8% and 32.1%, respectively. Most of the farms under study were small scale (94%) except a fe...

M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2* 

...lated from camel improve diagnosis, epidemiology and treatment of the organism causing diseases.

Keywords | Camel, Pasteurella multocida, Mannheimia haemolytica, Phenotypes, Genotyping, PCR 

...
Tabinda Urooj1,*, Bushra Wasim1, Shamim Mushtaq2, Syed Nudrat Nawaid Shah1, Lubna Faisal3, Moazzam Ali2, Nabeela Rasheed1 and Syed Faizan Ali Rizvi4
... by neglecting the early diagnosis of the disease. Also, higher risk was observed for married women, housewives with extended family that is 3 or more children. Breast feeding for extended duration (at least 2 years) and less age at first childbirth were observed as protective factors for this cancer. Also, majority of our study subjects were diagnosed at late stages of cancer (stage III and stage IV). Therefore, early detection will not only cure the breast c...

Mohammad Hussain Haidary1, Rozanaliza Radzi1*, Muhammad Waseem Aslam1, Seng Fong Lau1, Farina Mustaffa Kamal2, Ahmad Rasul Radzali1 

...utra Malaysia (UPM). The diagnosis of FCGS was made based on oral examination with the inflammation at the caudal oral cavity and lateral to the palatoglossal folds. Descriptive statistics were derived from the retrospective data using simple percentages. Fifty-seven cats were analyzed up to the final treatment with known survival status. The majority affected cats age between 3-6 years old (24/57) and male (41/57). Most affected cats came from indoor multicat...

Zheng Tan*, Li Li, Yuxin Song, Meirong Tian and Fan Jia

...ased during the clinical diagnosis of CHD, which indicates that these three indicators play a key role in the pathogenesis and can be used as indicators for clinical risk stratification of CHD.

...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ed to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practi...

Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ed to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practi...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...ld be prevented by rapid diagnosis either molecular or serological test. However, the later test is inexpensive such as heamagglutination inhibition test (HI), but IBV fail to give Heamagglutination (HA) reaction without pretreatment. Therefore, we designed this study for preparation of IBV antigen by treating with different enzymes for HA reaction. IBV local isolates were characterized by SDS-PAGE and RT-PCR. The indigenous isolate HA antigens were treated wi...

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...ic carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable and unacceptable for most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...a previously established diagnosis of «FeLV-infection and chronic leukaemia» (n = 10) and healthy control cats (n = 6), we examined the following: 1) Haematological parameters of the blood; 2) Biochemical blood indicators of the physiological characteristics of the kidneys and liver, whose disorders are most critical in the progression of the leukaemic process; 3) Replicative and expressive activity of provirus FeLV in blood white cells (WBC); 4) C...

Hong Liu and Rui Xu*

...ative basis for prenatal diagnosis of fetal renal blood perfusion in patients with gestational hypertension. It could also be used to detect risk pregnancy in early pregnancy.

...

Jocelín Selene Sánchez Cisneros1, Braulio Alejandro Fuantos Gámez1, Martin Herrik Ramón Kane1, Laura Miranda Contreras2, Camilo Romero Núñez2* 

...our characteristics. The diagnosis was cutaneous collagen dysplasia suggestive of cutaneous asthenia. This is the first report of cutaneous asthenia in a baby albino Burmese python in Latin America.

Keywords | Burmese python, Histopathology, Ehlers-Danlos syndrome, Wounds, Cutaneous asthenia 

...

Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2* 

...nal conditions. Mastitis diagnosis was performed 21 days after calving, then animals (n=90) were grouped to normal (n= 75), and clinical mastitis (n=15) groups. Results show that interval from calving to first estrus (31.60±0.86 vs. 33.10±0.98 d) or first service (55.20±0.97 vs. 61.00±1.96) was not affected (P≥0.05). Mastitis decreased (P<0.05) estrus/mating (93.3 vs. 80.0%) and pregnancy rates (86.66 vs. 53.33%), prolonged (P...

Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2* 

...ernative tools for early diagnosis of subclinical mastitis in goats. Also, there was a significant relationship between milk Hp and SCC, thus reflecting the intensity of the inflammatory response to mammary tissue.

Keywords | Acute phase proteins, Subclinical mastitis, Serum, Milk, Goats. 

...

A.J. Shoyombo1, O.O. Alabi, B.M. Falana1*, R.A. Animashahun1, S.O. Olawoye1, F.A. Okenniyi1, M.A. Popoola2, C.I. Ukim2, A.M. Ake, A.E. Jubril3 

Albina Gabdrakhimovna Darmenova1, Samat Ravkhatovich Yusupov2, Abzal Kenesovich Kereyev1*, Mirzabek Gashimovich Zukhrabov2, Yerbol Maratovich Sengaliyev1, Dosmukan Ermuhkanovich Gabdullin1, Berik Garipullievich Satybaev1 

...actions allows for early diagnosis of uterine abnormalities in the placenta stage and the postpartum period.

Keywords | Interval of uterine contractions, Postpartum endometritis, Retained placenta, Strength of uterine contractions, Uterine subinvolution, Device for determining uterine contractions. 

...
Neeranoot Detcharoenyos1, Nakrob Pattanapon1, Soontaree Petchdee2*
...n the clinical symptoms, diagnosis, and treatment of osteochondroma in a young dog.
 
Keywords | Cartilaginous exostosis, Dog, MRI, Osteochondroma, Total resection
...

Nawal Abd-elhafez Hassanain1, Raafat Mohamed Shaapan1*, Ahmed Maher1, Ahmed Badwi Yousif2, Gomaa Desoky Emam2 

...  

...

Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7

...lated to early pregnancy diagnosis in lactating Marecha she-camels, a contribution towards modern approaches in research of camel reproduction. As the Marecha camel is the main breed of Pakistan, playing very important role in rural economy of desert areas of the country, this specific study will be used to build the country’s data base for future research.
...

A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3 

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...ssay, and to confirm the diagnosis of COD, mostly trans rectal palpation and trans rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines.

...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...It could be used for the diagnosis and epidemiological investigation of toxoplasmosis in cats, thus laying a good foundation for preparing ELISA kits for clinical detection.

...

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

...uated for its potency in diagnosis of S. aureus mastitis through indirect Enzyme Linked Immunosorbent Assay (ELISA). For purification of fraction, bacterial fresh cultures were centrifuged at 3000 rpm / 20 min and were homogenate in phosphate buffer saline and centrifuged at 15000 rpm / 20 min that known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fracti...

Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2

...done using the tentative diagnosis by rt-PCR. The presence of 16 liver and spleen samples out of 20 (80%) from aborted fetuses were positive for EHV-4, but all were negative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. The virus glycoprotein B (gB) fragment (580pb) was amplified in selected isolates using the nested PCR (n-PCR), sanger sequencing of gB from three isolates with phylogeneti...

Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5

...ternative markers for TB diagnosis, and their possible involvement in disease pathophysiology. In the present study, we investigated the genetic association and frequency of CXCL9 and CXCL10 genes polymorphisms in TB patients of Pakistani population. A total of 260 study participants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with ...

Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3 

...accuracy of differential diagnosis of benign and malignant neoplasms, in particular of the mammary gland (MG). Methods. Surgical material obtained after unilateral mastectomy with simultaneous hysterovariectomy, as well as peripheral blood samples of cats with MG tumours was used. Blood samples and surgical material obtained from healthy cats after a planned hysterovariectomy were used as control. In cats with established diagnosis

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

...s. A timely and accurate diagnosis is critical for treatment in this regard. Expression and purification of HDAg, which is the only protein of HDV of the local isolate is the primary objective of the study. This antigenic recombinant HDAg protein can be useful for both vaccine development and as a diagnostic marker of HDV. After determination of HDAg antigenic region of HDV and its amplification, the fragment was cloned in bacterial expression vector. The expr...

Pharaoh C. Sianangama1*, Brian Tembo2, Sylvia J. Harrison1, Rubaijaniza Abigaba1,3 

...d  

...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

Syeda Batool Zehra1, Abdullah G. Arijo2, Aly Khan3, Nasira Khatoon1* and Samina Waheed1

...ion. Traditional pinworm diagnosis via PCR for pinworm detection has been performed. The findings in this research were based on two key factors: molecular analysis and statistical analysis. When traditional morphological techniques used with, molecular biology technologies it has proven to be effective in distinguishing closely related species. Statistical analysis is used to determine the intensity of infection in relation to age and gender. This study will ...

S. M. Ashraful Karim1, Md. Shohel Al Faruk2* 

... suffering from CKD. The diagnosis and management of CKD at all stages requires the use of substantial evidence-based guidelines and principles.

Keywords | CKD, Polyuria, Ultrasonography, Biochemical Parameters, Ultrasonography. 

...

Humera Manzoor1,2,5 Norbert Brüggemann2,3, Hafiz Muhammad Jafar Hussain1, Tobias Bäumer2, Frauke Hinrichs2, Muhammad Wajid4, Alexander Münchau2, Katja Lohmann2* and Sadaf Naz1*

...to determine the genetic diagnosis of an inherited contracture disorder. Whole-exome sequencing was performed for four participants. Variants were filtered based on homozygosity in the three patients and heterozygosity in the obligate carrier (mother), predicted effect of variants on the encoded protein, and their frequencies in public databases. Sanger sequencing was performed to explore the segregation of the variant with the phenotype. All patients had cong...

Ayma Aftab, Samia Afzal* and Muhammad Idrees

... can be helpful in early diagnosis of COVID-19 instead of relying on real time polymerase chain reaction (RT-PCR) that can give false negative result. Nasopharyngeal samples from a 22 years old man were detected as negative for COVID-19 for consecutive three RT-PCR tests. Complete blood count (CBC), D-dimer, serum ferritin, eosinophil sedimentation rate (ESR) test, tuberculosis test, real time PCR and high-resolution computed tomography (HRCT) were done to rul...

Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1 

... allocated for HPAI H5N8 diagnosis using rRT-PCR and the results were 66 positive samples with the highest incidence in the Giza governorate. The winter season showed the highest incidence with 28 positive samples. For more characterization, the full HA gene was sequenced, followed by phylogenetic analysis according to the year examined, mortality rate, positive results of RT- qPCR with low Ct value (H5 and N8 were 15 and 20 respectively). The phylogenetic tre...

Muhammad Shahid Nadeem*, Jalaluddin Azam Khan and Firoz Anwar

...ical applications in the diagnosis of many diseases. In the present study we have produced a recombinant of ALT from Pyrococcus abyssi in BL21 (DE3) strain of E. coli. The recombinant enzyme was purified by anion exchange chromatography, it displayed a 45kDa band on SDS-PAGE, with 58.1% final recovery, 15.3 fold purification and 139 U/mg specific activity. Maximum enzyme activity was found at pH 8 and above 90C, its KM and Vmax values were found 25µM L-a...

Laveena Shekhawat, Sheethal S* 

...tient which revealed the diagnosis. Results: The patient underwent laparoscopic deroofing procedure and the cyst was sent to pathology and microbiology sections for laboratory diagnosis. Identification of sickle shaped hooklets on wet mount and ZN staining confirmed the diagnosis. The patient received post-operative oral albendazole which he failed to start immediately leading to recurrenc...

A. Yu. Aliev1*, A.A., Aliev1, A.M. Musaev1, M.Z. Magomedov2 

...test indicator for rapid diagnosis of mastitis in ewes. In laboratory studies using the Al-test diagnosticum, the main criterion for the presence of subclinical mastitis in animals was the formation of a well-formed purple jelly-like clot (moderate to dense) of in the milk samples, which was partially (+) or entirely (++) of thrown of out the from the hole in plate with stirring. On the plate, with a negative reaction (-), the mixture of milk and the ind...

Esam A Razin1, Hassan Sobhy2, Tarek R. AboElnaga1, Asmaa A. Darwish1*, Rasha S. Mohammed1

...e biomarkers for disease diagnosis and its treatment monitoring. 
 
Keywords | Anti-trypanosomal activity, Basil extract, Clinicopathological alterations, Histopathological alterations, Diminazene aceturate
...

ASIMA BANO1, HAFIZ MUHAMMAD TAHIR*2, AROOSA RASHEED3, MUHAMMAD ARSHAD4, SHAFAAT YAR KHAN1 & RABIA YAQOOB5

...used as a marker for the diagnosis of breast cancer.
 
Key Words: Esterases, breast cancer, risk factors, diagnosis
...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

... used for identification/diagnosis of Xanthomonas axonopodis. Bacterial culture used as template in PCR and colony PCR gave better results as compared with extracted DNA from infected leaf.
 
Key Words: Xanthomonas axonopodis citri, Citrus canker, Molecular diagnostics
...
 
 MUHAMMAD MUNEEB SOHAIL1, FATIMA AMEER2, GUR CHARN SINGH2, MUHAMMAD ZAID1* 
...the characterization and diagnosis of diseases but it can prove helpful in assessing their severity and type. 
...
 
 ASMAT MEHMOOD, ZAEEMA KHAN AND NAILA MALKANI* 
.... The most common age of diagnosis for oral cancer was 45-65 years, and the standardized cancer incidence rate was 633.411 i.e. 8/1000000 with a confidence interval of 95%. The study concluded that oral cancer is significantly increasing in Pakistan, and there is a dire need to address this problem. In addition, we recommend the development of an appropriate nationwide system of cancer registry. 

Ahmed Abdel-Rady3*, Mohamed Karmi2, Menna_allah Youssef1, Aml M. Abdel-Ra’ouf1, Bahaaa Madkour1 

...ed animals. Conventional diagnosis based on blood and lymph smears examinations showed that, the prevalence of Tropical Theileriosis in cattle in Aswan Governorate was 56 (21.13%). Giemsa stained blood smears showed presence of macro-schizont inside lymphocyte (Koch’s blue bodies), micro-schizonts inside lymphocyte, raptured schizont and intraerythrocytic stages of Theileria annulata piroplasms inside RBCs..Polymerase chain reactions of T. annulata meroz...

Imran Khan1*, Shahid Amin2, Safiullah3, Shah Zeb Khan3, Abidullah3, Amjad Ali3, Saghir Imdad Hassan3 and Israr Ud Din3

...od samples were used for diagnosis. After analyses, 5.67% (17/300) samples were found positive for Trypanosoma evansi using thick and thin blood smears techniques as per method described by (Adam et al. 1971). A higher infection rate was found in females (6.67%) as compared to males (4.67%). Highest (9.00%) infection was noted in camels above 4 years of age group followed by (5.00%) in camel under 2 years age group while lowest (3.00%) in 3-4 years age group c...

Soedarmanto Indarjulianto1*, Alfarisa Nururrozi1, Yanuartono1, Sitarina Widyarini2, Dhasia Ramandani3 

...phadenopathy. The proper diagnosis and early treatment were needed to increase the chances of successful therapy.

...

Hala A. Amin1; A. Barakat2; A.A. Abou Zeid1

...t-align: justify;">Early diagnosis based on immunoenzymatic techniques requires significant amounts of immunoreagents. Hence, using directional cloning technology, a 38-KDa fusion protein containing a complete coat protein (CP) gene or Citrus tristeza virus (CTV) encoded by the expression vector plasmid (Pinpoint™ Xa3, Promega) with a fragment of biotin binding protein (BBP-rCTV-CP) was highly expressed and purified from E. coli cell culture. Antiserum o...

Sahar. A. Shoman1 and B. A. Othman2

...that will be helpful for diagnosis in mixed infection. In addition, it will be helpful in further molecular characterization of this strain.

...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...of our findings, routine diagnosis of STEC and improvement of hygienic measures must be considered as a critical concern for public health.

...

Sar Zamin Khan1*, Muhammad Waqas1 and Sagar M. Goyal2

...g with its pathogenesis, diagnosis, and control. In summary, we have discovered that measures to combat infectious bronchitis is not fully effective and the continued attention of the scientific community and funding agencies can help protect the global poultry industry from this challenge.

...

Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin 

...kers in the differential diagnosis of sex cord-stromal tumors in the ovary of dromedary camels. 

...

Kinza Javed1, Muhammad Ali1, Waqas Farooq2, Majeeda Rasheed1*, Farkhanda Yasmin3, Samiya Rehman3, Sadaf ul Hira1 and Jawad Ali3

...ogy, host vulnerability, diagnosis, preventiand therapeutic approaches.  Lumpy skin disease virus belongs to Poxviridae family and genus, Capripoxvirus. It cause acute to fatal infection in cattle. Lumpy skin disease results in loss of weight, infertility, abortion in cows and reduction in milk production. In this way this disease has a serious threat to economy of Middle East, European and African countries due to loss of meat and dairy products. LSD is ...

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

.... albicans isolates. The diagnosis was confirmed using 25 of the most virulent C. albicans isolates, by polymers chains reaction (PCR) to detect the KER1 gene revealed that all 25 samples were positive with the product size (658bp). Phospholipases B1 (PLB1) and the secretion of aspartyl proteinase 1 (SAP1) gene were used to detect C. albicans virulence with 100% and 80% accuracy, respectively, while Hyphal Wall Protein 1 (HWP1) gene was not detected. It is exp...

Moustafa M. Zeitoun 1,3; Mohamed M. Mansour 2,4* 

...a testosterone for early diagnosis of sub-fertility in male camels. 

...

Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2* 

...purpose of isolation and diagnosis resist.E. coli 0157:H7 two hundred urine samples were collected from adult women patients suffering from UTIs during a period from February 2022 to July 2022 in Diwaniyah city Iraq . All urine samples were incubated aerobically at 37°C for 24-48 hrs on blood agar, MacConkey agar as well as biochemical tests identified special media and the isolates. The isolates were confirmed and diagnosed by Vitek assay. In this study,...

Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar

...ods that allow for early diagnosis and care for better outcomes is critical.

...

Khadija Begum, Azizunnessa and Md Ahaduzzaman*

...anding the epidemiology, diagnosis, and clinical management of reproductive disorders in goats. 

...

Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li

...ction method for disease diagnosis, clinical monitoring, and treatment of BVDV, BRV, and BCV infections.

...

Swagata Das Gupta1, Majharul Islam1, Towhida Kamal2,Md. Rayhan Faruque3,Md. Shohel Al Faruk4* 

Asmaa A Darwish* 

...kers for urine retention diagnosis and IL-1α, TNF-α, Fb, and ferritin as the best predictors for the urethral process amputation results. The study concluded that urine retention in rams resulted in a strong immune response with subsequent hemato-biochemical changes. TAC and IL-1β are reliable biomarkers for urine retention in sheep while, IL-1α, TNF-α, Fb, and ferritin are better for urethral process resection outcomes prediction....

Ferry Lismanto Syaiful1*, Jaswandi Jaswandi2, Mangku Mundana2, Ilham Ilham2, Novirman Jamarun1, Efrizal Efrizal3

Abdul Kabir1*, Anees Ur Rahman2, Inzamam Ali Shah2, Ibad ur Rahman2 

...mics, clinical features, diagnosis, prevention, and control of buffalopox, with emphasis on the interplay between buffaloes, humans, and vectors. The review also highlights the gaps in research and the need for improved surveillance, molecular characterization, and vaccine development for buffalopox.
 

...

Zhimin Wu1,2, Yaping Yang1, Arshad Zahoor1, Aftab Shaukat1 and Ganzhen Deng1,2*

...plied widely in clinical diagnosis of canine pulmonary diseases for the sensitive and direct perception to the abnormal changes. Due to few of relevant reports about different measurement methods and indexes, the simple radiographic findings are difficult to be used in accurate clinical evaluation. This test aimed to develop a radiographic method of comparing canine posterior lung field angles include vertebrophrenic angle (cranial), vertebrophrenic angle (cau...

El-Kenawy, A. A. and El-Tholoth, M. S.

... are useful in the quick diagnosis of the disease in naturally infected cattle. PCR could be used for rapid and specific detection of LSDV nucleic acid in crude skin and internal organs samples. Also, LSDV could be detected in CAMs of ECEs  using PCR at first day post-inoculation (PI) and by IF and IP at second day post-inoculation before appearance of characteristic pock lesions on CAM.

...

Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

...ridization technique for diagnosis of bovine ephemeral fever virus.

...

Abo-Elmagdl , Enas K;El-Mougy2, Hala M. T. and Abd El-Salam 3, Manal

...sted. Rapid and accurate diagnosis of RSV infection is crucial for appropriate patient management and infection control. The aim of our study was to compare the diagnostic performance of Binax NOW RSV (BN) as a rapid test for detection of RSV antigen in nasal wash specimens collected from Egyptian infants and young children with the detection of RSV-RNA by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RSV infe...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K  and  Mona M.Aly 
 
...outbreaks of 2006. Viral diagnosis was based on direct detection of viral RNA by real time PCR. Two positive cases from turkey and Grocer duck were processed for viral isolation and characterization. The level of antibodies was detected in vaccinated birds by HI test and the results were discussed to evaluate the role of vaccination in controlling the disease in these valuable zoo birds.

...

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

...e candidate for improved diagnosis and control rotavirus infection in young calves.
...

A. A. Kheder1; I. A. M. Ibrahim2; H. M. Mazyadl

... molecular procedure for diagnosis. Electron microscopy of purified preparation of PRMV showed presence of isometric particles 28 nm in diameter. Ultrathin section for electron microscopy examination of infected peach leaves shows virus particles in vacuoles. Tubules structure scattered in the cytoplasm or associated with plasmodesmata was shown. Extensive severe degeneration of chloroplast and mitochondria structure as well as development of cell wall protrus...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...es for the detection and diagnosis of trypanosomosis. To create a recombinant antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence, after which the gene was synthesized using solid-phase method. The gene fragment, encoding the GM6 protein, was inserted or cloned into the pET28 expression vector after synthesis. The obtained ...
Asad Ullah1, Hayat Ullah2, Muhammad Lateef3, Imrana Niaz Sultan4, Afrasiab Khan Tareen4 and Muhammad Waseem Khan4,5*
... techniques used for the diagnosis of TB. Our study is one of the first ones from Pakistan investigating and assessing the performance of GeneXpert. We recruited eight hundred clinically TB suspects initially and included seven hundred and sixteen clinically TB suspects in the final analysis. The results of GeneXpert were compared with Mycobacteria Growth Indicator Tube (MGIT) and Ziehl-Neelsen (ZN) staining. In comparison to MGIT and ZN staining the sensitivi...

Kadhim Kh. K. Al-Khayat1*, Athmar K. A. Al-Azawi2

...of cysts. To confirm the diagnosis of C. pisiformis (scolex and part of membrane) from these cysts were put in a 70% ethanol for further used in conventional molecular diagnosis.  The results  revealed that an overall infection rate was 23.33% (14/60). The females infection rate was 27.90% (12/43) and in the males 11.76% (2/17) without significant differences (P≥0.05 ). Fifty-five cysts  were isolated from ...

Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3

...psy techniques for early diagnosis in order to proceed with the appropriate treatment.
 
Keywords | Buffaloes, Uterus, Infertility
...
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
...her for the conventional diagnosis procedure. All isolates were identified as Avibacterium paragallinarum and required nicotinamide adenine dinucleotide phosphate (NAD) for their growth. The isolates were typical for Av. paragallinarum as they were not able to ferment neither galactose nor trehalose. Performing PCR of direct swab samples provided higher detection level as compared to the PCR from isolated colonies following conventional diagnostic procedure. I...
Nusrat Bano1, Muhammad Tayyab1*, Bushra Muneer2, Sehrish Firyal1, Abu Saeed Hashmi3, Muhammad Wasim1 and Ali Raza Awan1
...e patients for the quick diagnosis and detection of dengue stage for the proper treatment of infection. This cross-sectional study was conducted at Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore and Jinnah Hospital Lahore (JHL) from January to December 2013. Total 345 clinically suspected patients reporting to JHL were serologically diagnosed for dengue specific antigen and antibodies. In this study 20 patient...

Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain

...nd on a clear and prompt diagnosis. For the diagnosis of Rabies, conventional techniques such as Direct Fluorescent Antibody Test (dFAT) and histopathology are still employed. The OIE and WHO both frequently propose the gold standard test, the Direct Fluorescent Antibody Test (dFAT), for the diagnosis of Rabies in fresh canine brain tissues. The preferred methods for routine

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

... is the timely and rapid diagnosis of the disease, which will allow the timely implementation of preventive measures. The purpose of the study was to develop an express test to identify cases of animal morbidity for emphysematous carbuncle in the field. For this purpose, a serological method was used using the latex agglutination test (LAT). When preparing the diagnostic, antibodies to surface proteins associated with the flagellar antigen Clostridium chauvoei...

Thuong Thi Nguyen1*, Nhu Quynh Ho1, Thuan Khanh Nguyen2, Tuan Phong Vu Anh Vo3, Hai Thanh Duong4

...orders and support early diagnosis of mastitis, ketosis, and abomasal displacement in the dairy cow farm.
 
Keywords | Alerts, Dairy cows, Milk yield, Rumination, SCR systems
...

Karin Alvåsen1*, Karin Maria Olofsson2, Raymond Kent Fenton3, Ulrika Rockström4, Lena-Mari Tamminen1, Nils Fall1

...ropsied animals, the RDN diagnosis matched well with the on-site veterinarian’s observations. The participants expressed a great interest in developing and obtaining more skills in performing on-farm necropsies. This pilot study identified several challenges with the RDN and these need to be considered before implementing this service for Swedish cattle farms. The pilot study also showed that the RDN method could be a good complement to the regular post-...

Advances in Animal and Veterinary Sciences

June

Vol. 12, Iss. 6, pp. 994-1205

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