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Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*

...;The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed that highest titers were seen in animals of age > 2 years i.e. 1...

Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2

...alculated geometric mean titers of 5.37 revealed the infectivity of the flock with H9N2 Influenza virus. To investigate the presence of virus in the study population, trachea, lung and intestine tissue samples were processed for RNA isolation and subjected to molecular detection of H9 gene of H9N2 subtypes using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Out of 24 samples taken from each organ, 22, 21, and 18 samples from trachea, lungs...

Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5

... an increase in the mean titer of antibodies was recorded from 17 and onwards. Furthermore, on day 39 of age, the birds of all groups including control were challenged with field strain of IBD virus collected from bursa of clinically infected chicks, No morbidity and mortality were observed. During present study, some milder infection with field strain of IBDV almost a week ago recorded were also found in the control group when challenged with IBDV, IBD vaccin...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...us neutralizing antibody titer (mRVNA). All puppies were whelped by dams regularly vaccinated with high quality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declare...

Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...s shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in eggs. Data indicated that all chickens vaccinated with pCA-Egy-H9 were fully protected without clinical signs and virus shedding. Our results revealed that the pCA-Eg...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...of 0.1ml of EID50 (virus titer 105.50/100μl) on 2nd week of age through eye drops. Group B specific antibody negative (SAN) chicks were challenged with same dose at 2nd week of age. While the group C and D was unchallenged control containing commercial and SAN chicks. The Blood samples were collected from group A, B, C and D on 3rd, 5th and 7th day of post infection for biochemical analysis. ...

Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2

... age, the Geometric mean titers (GMT) for H5 were increased from the 7th day of age using both antigens (RE-5 antigen) and (S75/Egy/2015 antigen). Generally, GMT post-challenge was increased as the chicks overcome the infections. In conclusion, our results indicate that vaccination at 5th day old with baculovirus H5+ND inactivated vaccine can interfere with MDA of H5 vaccine and has negative impact on H5 vaccine titer. Howev...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...d detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA vaccine induced complete protection (100%) and there was no virus shedding in all chickens immunized with the Egy-H5 plasmid DNA vaccine. Taken together, the presented...
Naila Chand1,*, Shabana Naz2, Hamza Maris1, Rifat Ullhah Khan1, Sarzamin Khan1 and Muhammad Subhan Qureshi1
...cytes ratio and antibody titer was significantly (P<0.05) lower in the treated groups. Based on these results, it is concluded that supplementation of diets with betaine improved broiler feed intake, weight gain, FCR and increased immunity were under heat stress condition.
...

Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1 

 

...D274 and Qx types, while titers for vaccine B were not detected, suggesting a role of the poor emulsion quality on the performance of vaccine B. These results demonstrate that effective quality control and emulsion preparation are essential benchmarks of immunization and vaccine potency. 

...
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
...er parturition. Antibody titer was determined by Complement fixation test. Results showed that highest antibody titer was developed in cattle, colostrum and their calves in group G in which treatment was started 75 days prepartum and VESS was given. It was concluded that vitamin E and selenium supplementation improves both active and passive immune system if given before 75 days of parturition.
...
Zia-ur-Rehaman1, Naila Chand1, Sarzamin Khan1 and Rifat Ullah Khan2,*
... 42. Mean serum antibody titer against Newcastle disease (ND) and serum paraoxonase (PON1) were significantly higher (P< 0.05) in TN zone, while serum malondialdehyde (MDA) was significantly (P< 0.05) higher in HAT zone. No significant differences were recorded in serum antibody titer against ND, PON1 and MDA among different broiler strains in TN zone, while in HAT zone, Ross and Arber Acer showed significantly (P<0...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...ls had high IgG antibody titers to CPV prior to vaccination and vaccination did not result in increased titers. A decline in CPV antibody titer with increasing age was observed regardless of immunization status. Our results suggest that vaccination of African wild dogs with a live attenuated domestic dog vaccine may provide adequate protection against CDV, and could therefore be an importa...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) of the samples were positive for viral matrix gene. Sub-typing was performed by specific anti-sera. PCR amplification was done by using N2 specific primers that confirmed 100% of the matrix (M) gene positive isolates as subtype H9N2. This is the first report from Pakistan that confirms prevalence of AIV H9N2 among different bird sp...
Hazrat Nabi1, Irshad Hussain2, Muhammad Adil3, Amar Nasir3,*, Arbab Sikandar3, Saeed Khan1 and Nasrullah Khan3

 

...oxin binders on antibody titer against New Castle disease virus, feed consumption, weight gain, relative weights of lymphoid organs and feed conversion ratio (FCR) of broilers were investigated. Chicks fed aflatoxins (group B) alone manifested significantly reduced feed consumption, body weight gain and feed conversion ratio. However, no variation in weight gain and FCR was found between the controls (A, B and C) and mycotoxin binders fed groups (experimental ...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...ups (n=18). The antibody titers were evaluated with indirect haemagglutination test. The results indicated significantly higher (P< 0.05) immune titer in animal group vaccinated with toxoid vaccine prepared from pathogenic isolates and provide best protection against challenge infection.
...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...up>C as no drop in viral titer was witnesses in bacteriophage samples stored at 4oC for up to one year. The results of this research indicated, that a standard refrigeration is suitable and economical means of long term storage with no fall of phage activity.
...

Bata Shalangwa Ishaku1*, Maimadu Abdullahi1, Dakwang Nalong1, Rengkat Jonah1 and Olabode Mayowa

.... Serum samples with LAT titer >10 IU/ml were considered positive. The study showed that 176 of the 500 samples analyzed were positive for Toxoplasma gondii antibodies (IgG) giving an overall prevalence of 35.2%. There was statistically significant difference in the T. gondii seroprevalence based on the animal species (P=0.023; odds ratio=7.688) screened in the study. In pigs, T. gondii seropositivity was found to be higher (46.2%) and about five times more...

 Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1

Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...for H9. The
virus titer was ranged from 1:64 to 1:512. The body weight gain of FO
supplemented broilers was higher than broilers receiving cooking oil (CO) while the
FCR of FO treated broilers was significantly lower than broilers receiving diet with
CO before virus inoculation, furthermore a significant difference was observed in
FCR after challenged, with highest value of group A, followed by group D, B and C.
The Ge...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
... castle disease antibody titer were significantly higher in basil supplementary group. The weight gain and feed conversion ratio significantly improved in basil treated group, while ascorbic acid and basil significantly decreased the water intake. Body temperature and mortality percentage were significantly decreased in ascorbic acid and basil supplementary group as compared to control. Dressing percentage significantly increased in both supplementary groups. ...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...lus). The antibodies titer in rabbit after immunization in 100% pure serum sample was 0.736 which was 15 times more than that of pre immune control sera (0.049). It is concluded from the current study that purified HCV3a envelope glycoprotein E2 has significant antigenic activity and can potentially be used as recombinant vaccines against HCV infection.
...
Mumtaz Ali Khan1,*, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Ikramul Haq4, Kashif Prince1, Asad Ullah5, Muhammad Shoaib2, Shahid Zaman6, Amjad Islam Aqib7, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4,Imad Khan5, Naimat Ullah4 and Muhammad Shahid8
...ts and goats. The immune titer against C. perfringens type D was evaluated on 0, 7, 14, 28th and 60th day of vaccination with indirect haemagglutination test (IHA). The results showed significantly higher (P< 0.05) immune titer in vitamin E supplemented animals groups against C. perfringens type D epsilon toxin.
...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
... had highest HI antibody titers (7.22±0.25, 7.36±0.18 and 6.55±0.38). In experiments 1 and 2, supplementation with immune modulators had significant (P<0.05) effects on total SRBC, IgG and IgM titers. In conclusion, vitamin E showed better immuno-modulatory effect followed by vitamin C, nucleotides and DNA, respectively. Supplementation of immuno-modulators at early age (1 to 21 days) showed more prom...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (NDV+IBV-challenged), D (IBV+NDV challenged), E (NDV and IBV- challenged) and F (negative control). Mild to moderate clinical presentation...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
...on Based on IgY antibody titer, IgY thermostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was relatively...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...Brucella antibody titers. In conclusion, brucellosis is prevalent in Islamabad Capital Territory.
...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...agglutination inhibition titer of all experimental groups was found higher on day 21 as compared to day 0 and 7. Taken together, experimental data showed the susceptibility of Japanese quails and mynas to experimental intramuscular and oral inoculation with VVND virus. Moreover, clinical VVND does not occur in Japanese quails upon contact exposure to VVND virus infected chickens although there is sero-conversion.
...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
...r anti-MG-ELISA antibody titer. Cumulative mean anti-MG-ELISA antibody titer of birds to G-MG, O-MG, G-MG+NDV, O-MG+NDV and control was 80.30, 137.49, 91.59, 169.76 and 0.15 units respectively on 35 days post-vaccination. It is concluded that NDV has insignificant immunomodulatory effect on antibody response of birds to the MG vaccine.
...

Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1

... ethanol and lactic acid titer from wheat straw. Adaptation of the fermenting strains reduce fermentation time and enhance the product yield
...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...P<0.05). The antibody titer against Newcastle disease virus and sheep RBC showed no significant difference among treatment groups (P>0.05). Supplementation of herbal extracts as feed additives at 200 mg/Kg produced better growth performance of broiler chickens with no adverse effects.
...

Walaa ,S. Shabanaa;Bakr, A. Abdallaa; Eman, M. ELShalamia; I.M. Redab; M. A. Shalabyb

...>
mean of antibody titers continued with the protective level till the 32 to 36th week post vaccination,
In single vaccination either FMD or RFV alone while till to 40th WPV in combined FMD/RVF
vaccine.In the final we can conclude that the use of Montanide ISA 50 as an oil adjuvant in prepared
vaccines improve the immune response against FMD and RVF, giving high titer of antibodies
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...nt experiment. The virus titeration assay showed a marked decrease
from 105 TCID50/ml to 103 TCID50/ml in adsorption step simultaneouslly incubated
with Salvadora persica extract . In contrast, the plan extract didn’t show any activity
against BHV-1 neither in pre- nor post- treatment experiments. Indeed, the present study
confirms that ethanolic extract of Salvadora persica roots had an antiviral effect ag...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...test. The serum antibody titers were determined by serum neutralization test (SNT) and Enzyme linked immunosorbant assay (ELISA) in calves and also their dams. The results confirmed infection of calves with lumpy skin disease virus (LSDV) and showed that calves older than 3 months old age and those out of first calf heifers are more susceptible for infection. Some calves even with insufficient amount of maternal antibodies could be protected from infection. In...

Walaa Abd El-Fatah S. Metwally1, Ismail M. Reda2, Ahmed A. El-Sanousi2, Mohamed Abd El-Khalek Ali2

...se were high in antibody titer than vaccines containing 256 and 100 HA unit/dose. The prepared vaccines with Montanide ISA 70VG containing 256 HA unit/dose gave high antibody titer than vaccines containing 100 HA unit/dose. The ratio of 30% Chicken strain of AI to 70 % Duck strain of AI gave the best result of antibody titer followed by the ratio 40% to 60% followed by 50% to 50% respectiv...

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

...s shown in mean antibody titers against Avibacterium paragallinarum serovar A, B and C and infectious bronchitis. In addition, Montanide ISA-70 is lesser in viscosity that makes it easily injected, so, it is better to use for control of infectious coryza and infectious bronchitis diseases in chickens.

...

1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed

... show the least antibody titer. The group of sheep, which was vaccinated with RVF, inactivated PMO without gel vaccine show intermediate level of antibodies. Moreover, the values of ED50 of the developed vaccines as well as the Alum vaccine alone were not exceed 0.02 which lies within the permissible level.

...

Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4

...e last injection had the titer of 1:2048 and 1:1024, respectively by indirect-enzyem linked immunosorbent assay (I-ELISA) technique. The produced antisera were evaluated and compared with foreign antisera using tissue printing immunoassay (TPIA) and dot blot immunoassay (DBIA). The produced antisera were found more efficiency in development the purplish blue color than the positive reaction when the foreign antisera applied, therefore it should be use produced...

Eman A. H. Kattab1,3, A. H. Ebrahem2 , Om-Hashem M. El-Banna2 and Hanan F. EL-Kammar³

...gainst BBMV have maximum titer of 1:1024 from the first bleeding. IgG and IgG conjugate were 1.0 μg/ ml and 1:1000, respectively. The produced antiserum was evaluated by DBIA and TBIA using infected plant.

...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3 

Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3

...a samples to measure the titer of
antibody against strains A,O, SAT-2 with high titer of antibody against serotype (A) was 87 ,
serotype (O) was 72 and serotype (SAT-2) was 53 . Matching between SNT and ELISA was
applied for negative sera samples.
...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...ons for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune sera for each virus by virus neutrization test (VNT). As the Neutrization index (NI) for PPV and FPV isolates were 2 and 2.75 when neutrized by FPV hyperimmune sera (HIS) while it was 2.5 and 2 when neutrized by PPV (HIS). DNA was extracted from the produced pock l...

Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar

...ls of CAV ELISA antibody titers in all the vaccinated chicken subgroups at 4th WPV with either DW or S/C CAV vaccine indicating that the two CAV vaccines were immunogenic; Also, by measuring H5N1 HI titers within the 6 weeks post vaccination with AI vaccine in the sera of different chicken subgroups using HI test. It is clearly observed that AI HI antibody mean titers in S/C and in DW CAV ...

Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed

...give protective antibody titer, while 23/200 gave non protective titer and 87/200 have no antibody againest LSDV. The results of clinco-pathological revealed highly significant increase in ALT, AST, ALP, GGT, urea and uric acid, while the level of total protein, albumin and calcium showed significant decrease and non-significant reaction in creatinine and non-organic phosphorus in infected cattle. The results of antioxidant ...

Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.

...PCR in which the H5 gene titer was
7.364, 7.499, 7.767, 8.049, 8.000 logs 10 copies / ml respectively. The result of in- house ELISA was
not significant for H5 gene detection and titration using polyclonal H5 serum. The HVT titer in CEF
was 3045, 3200, 3400, 3750 & 4000 PFU/dose for batch A, B, C, D, and E respectively. The selected
batches safety was satisfactory by 10 fol...

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

.../div>
Results: the titer of original HPAI virus was 1010.3 EID50/ml. The AI titers after experimental
infection of chicken and viral re-isolation were 1010.3 EID50/ml in ECE and 1.6×107 copies/ml by
rRT-PCR to sample (1), 105.4 EID50/ml in ECE and 1.2 ×106 copies/ml by rRT-PCR to sample (2),
and 106.2 EID50/ml in ECE and 4×105 copies/ml by rRT-PCR to sample (3). The...

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...showed the highest virus titer (5.952 x
106 and 3.245 x 106) than Vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x
105) after both 48 and 72 hours respectively.
Conclusion: The chicken embryo kidney cell (CEK) is the most successful and sensitive monolayer
cell culture host system for isolation of the Egyptian variant 2 strain of infectious bronchitis virus
(IBV)
...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...each protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported reassortant vaccine with 500HAU antigen content of H5N1 at 1, 5 and 10
days of age; respectively. A group 7 was positive ...

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

... detectable TRT antibody titers by SNT in the first week post vaccination as 4 log2
in group-1 and 2 log2 in group-3. Both groups showed peak SNT titer (256 log2) of TRT virus by the
third-week post administration of the second dose and remain stable up to 24 weeks post vaccination.
Follow up avian influenza antibody titers using HI test, peak

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

...eased virus severity and titer compared with the
control. However, the potential of wind velocity lower or higher than 2.6 was less effective on CMV.
Additionally, the severity and titer of CMV were significantly reduced in GF19-1 treated plants in
comparison with the control. Quantitative real-time PCR results exhibited increased transcription
levels of pathogenesis related ge...

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

...sease severity and virus titer were estimated at 1, 2 and 3 weeks after virus inoculation. The transcription profile related to defense genes was evaluated at 1, 2 and 3 weeks after BYMV inoculation by quantitative real-time PCR analysis.
Results: Treatments with PGPF resulted in lower virus severity and titer than the control. GP17-2 treatment exhibited the lowest disease severity and virus

Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1

...sease severity and virus titer were evaluated in cucumber plants treated with barley grain (BGI) or culture filtrate (CF) of GU23-3 under greenhouse conditions. Additionally, the expression levels of defense genes were assessed by reverse transcription PCR (RT-PCR) analysis.
Results: Both of virus severity and titer were significantly reduced in treated plants in comparison with the control. All growth characters...

Youssef A. Elgharbawy1, Mervat M. Ali1, Ayman H. El-Deeb2, Mohamed A. Shalaby2

...) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37°c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD w...

Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein

...ecline. Whereas the peak titers for antibodies were reported at the 28th day post vaccination. Indeed, the study reports the role of ail adjuvant in stimulating the cellular response for inactivated vaccine.

...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...ant (P<0.05) antibody titer increase from 1:16 - 1:1024 and mean
GMT 2.87 - 5.09 amongst 61.5 - 87.0% tested chickens. Questionnaire further showed V4
thermostable ND vaccination had significant effect on morbidity, mortality, hatchability, culling and
ultimately the flock productivity.
Conclusion: Corn-bran feed based vaccination demonstrated effective delivery and suitability of V4
thermostable N...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...d cell degeneration with titer of 105.5TCID50/ml,
DNA extraction and PCR for the candidate strain was performed using universal and specific primers
for virus identification and confirmation. The size of the produced amplicons was 192 pb for the
universal primer and 502pb, 1452pb for the specific primers respectively. Metagenomics is done to
evaluate a virus harvest for standardization by applying a suite of geno...

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

... both cultures, the LSDV titers were 5.2 in MDBK after 48hr and 5.67 in Vero
after 72hr. This rise in virus titer was followed by a sharp decrease after 48hr P.I. on MDBK unlike
Vero cells that continue its rise in virus titer until reaching its peak at 72hr P.I.
Conclusion: Comparison of growth kinetic of LSDV on MDBK and Vero cell line revealed that Ve...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
...FV neutralizing antibody titers.
Conclusion: The vaccine developed based on small particle vaccine formulation is an economic
solution that can be added inline to existing production platforms to enhance immunogenicity.
...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...
induced higher HI titer by using GVII monospecific antigen and decreased virus shedding
than other group. The vaccinated group with genotype VII significantly reduced the virus
shedding at 3, 5, 7 and 10 days post challenge unlike the genotype II vaccinated group that
did not reduce virus shedding and increased morbidity and mortality in contact birds.
Conclusion: The combined inactivated and live NDV vacc...

Muhammad Khubaib Hameed, Muhammad Aziz ur Rahman, Muhammad Ashraf, Safdar Hassan, Muhammad Riaz, Muhammad Qamar Bilal, Fawwad Ahmad, Muhammad Sharif*

...tion. Higher (P<0.05) titer against ND virus and IBD virus were recorded in birds fed diet containing arginine at 0.6%. It can be concluded that inclusion of arginine at 0.6% had improved growth performance and carcass yield.

Keywords | Arginine, Growth parameters, Carcass attributes, Immune functions  

...

Amjad Ali Channa1, Mansoor Tariq1*, Zaheer Ahmed Nizamani1, Nazeer Hussain Kalhoro2 

...ively. The mean antibody titer against H5, H7 and H9 virus was 4.87, 3.72, and 5.31 log2 respectively. AIV-ELISA revealed that overall prevalence of AIV viruses was 8.44% which was subtyped through RT-PCR that revealed the higher occurrence of H9 virus (5.33%) as compared to H7 (1.16%) and H5 (0.31%). However, the coinfections of H7/H9 (0.73%) was higher than H5/H9 (0.21%) and H5/H7/H9 (0.21%). In conclusion, the H5, H7, and H9 avian influenza viruses are cir...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...d to give significant HA titer up to 7 log2. During stability test antigen prepared by phospholipase C were found most stable up to six month by giving constant 7 log2 HA titer, while neuraminidase induced antigen were stable up to five months (7 log2). Trypsin treated antigen were readily lost its activity from 7 log2 to 2 log2 after two months of incubation. During specificity test all antigens showed specific effect on IB...

Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain

... anti-HA antibodies. The titer of antibody produced in immunogenic mice was determined through ELISA. The highest geometric mean titer of HA-specific serum IgG, which was greater as compared to the control.

...

Jun Zhang1*, Xiaocao Xu2 and Min Cao2

...% of patients the HBs-Ab titer was more than 10. Due to the progress of screening and selection of blood donors the prevalence of HBV infection in high-risk patients has sharply decreased in recent years. Vaccination is one of the most important protective factors in this group of the population. Therefore, administration of a reminder dose based on antibody titer is recommended in these individuals.

...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

...n (GM) log10 HI antibody titer (3.46 ± 0.06) at the 42nd day. Whereas calves in the single-dose immunized group reach the peak GM 1og10 HI titer (2.79 ± 0.13) at day 28th post immunization (p < 0.05). Challenged calves showed the lowest mean clinical respiratory sign score in booster-dose immunized calves (1.2 ± 0.35) than in the single-dose group (4.2 ± 0.51) but the highest score was noted in...

Sania Subhan Qureshi*,1, Baitullah Khan1, Shahid Khan1, Hanif Ur Rahman1 and Muhammad Subhan Qureshi2

...irus serotypes and virus titer producing CPEs on BHK-21 cells.
...

Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*

...d Newcastle disease (ND) titers. Accordingly, OAS’s as natural feed additives could improve broiler performance and immunological state. Chicks fed SF or SP had the best productive performance, immunological status, digestive parameters, and microbiota.
 
Keywords | Blood parameters, Broiler chicks, Gut health, Immune status, Organic acids salts, Productive performance
...

Tri Untari1*, Sitarina Widyarini2, Michael Haryadi Wibowo1, Marla Anggita1 

...asuring the NDV antibody titers using hemagglutination inhibition test and histological studies to observe the toxic effect of red ginger to the chicken livers and kidneys. Antibody titers in group I, II, III and IV were determined as HI (log)2 average and were recorded 3.81, 2.91, 3.13 and 0.95, while the geometric mean titers (GMT) were 13.9, 8.6, 7.5 and 1.9 respectively. The unvaccinat...

Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5

...nhibition geometric mean titers. Flavomycin and zinc bacitracin did not adversely affect the mean splenic (1.27+0.20 g and 1.21+0.15 g, respectively), thymic (3.42+0.26 g and 3.78+0.48 g, respectively), hepatic (21.78+0.83 g and 23.15+0.37 g, respectively), or bursal (1.55+0.79 g and 1.63+0.21 g, respectively) body weight ratios. However, lincomycin did adversely affect bursal (0.91+0.12 g), but not splenic (1.21+0.23 g), thymic (3.52+0.36 g), or hepatic (23.7...

Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy* 

...ck farms production at a titer of 3×106 CFU (Colony Forming Unit) /cm2 in the absence and presence of organic matter (O.M). The results showed that the efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000® was the most powerful disinfectant against field pathogens, followed by G7®, Pron-Tech®, and Alkadox®, while Biodine® was the weakest disinfectant at the same conditi...

Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2  

...vaccination with varying titers. Overall, the serological response at challenge day were significantly with higher titers in groups vaccinated with inactivated NDV genotype VII vaccines when compared with other groups. Regarding broiler performance parameters it was noticed that, there is no significant difference in body weight (BW) as well as feed conversion rate (FCR) among all chickens in different vaccinated groups. Fro...

Anum Syyam1,2, Nazish Saqlain1, Sidra Hareem1, Naghmana Mazhar1, Liaqat Ali3 and Samia Afzal2*

...nti-B antibodies and the titer of these antibodies among blood group O donors at a tertiary care hospital. This is a cross-sectional study conducted at the department of Hematology and Transfusion Medicine in Children’s Hospital and the Institute of Child Health, Lahore, Pakistan over a period of four months from Oct, 2019 to Feb, 2020. Blood samples were collected from 350 healthy and voluntary group “O” donors (males = 320 and females = 30,...

Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan 

...05) reduced the antibody titer against sheep red blood cells (SRBC), total protein, albumin values in broilers serum compared with ngative control. Aflatoxins supplementation significantly (P<0.05) increased malondialdehyde values in liver and significantly (P<0.05) diminished the reduced glutathione (GSH), glutathione S- transferase (GST). In addition to, dissemination of aflatoxin residue in broilers liver was detected. Nevertheless, dietary addition o...

Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2 

...noglobulin, and antibody titer, while reduced lysozyme. All PEE levels improved antioxidants capacity, while reduced malondialdehyde in blood serum and in seminal plasma. Advanced motility, membrane integrity, livability and sperm viability were improved, but sperm abnormality, apoptosis and necrosis were reduced in cryopreserved semen by all PEE levels. Total antioxidants capacity was increased, while malondialdehyde was decreased in post-thawed semen by all ...

Chanathip Thammakarn1*, Sawanya Umphonphison1, Paitoon Kaewhom2, Kanokrat Srikijkasemwat1 

...10.29% had high positive titer. On the other hand 8.54% of pig had no antibody to PCV2. The low positive antibody level was found in most population in all farm sizes as well as all types of pig as in age categories. The PCR result illustrated that there was no viral gene in seronegative serum. While 0.8% of total low positive serum samples displayed positive by PCR. In addition, 3.23% of total high positive serum samples showed positive by PCR. The present st...

Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1 

...st to determine antibody titers. The Haemagglutination inhibition test was done using Newcastle Disease Virus LaSota strain NLT 106.0 EID50 antigen. Post-hatch feed and water deprivation durations significantly affect the production parameters. Feed intake, body weight gain and average daily gain were significantly (p<0.01) lower in the T5 group. Significantly (p<0.01), a poor feed conversion ratio was observed in the T5 group. The lowest (91.66%) surviv...

Ayesha Bakhtiar1, Sardar Azhar Mehmood2, Abdul Rauf Bhatti3*, Shabir Ahmad2, Naqash Khalid2, Javed Iqbal5, Azra Nadeem4 and Waqas Ahmad1

... displayed high antibody titers throughout the experiment. In terms of geometric mean titers, there was a significant difference (p 0.05) between the groups. On days 7, 14, 21, and 35 of the experiment, data on other performance indicators, including as feed intake, water intake, total body weight, and feed conversion ratio (FCR), were gathered. Throughout the course of the trial, there were significant differences in these ...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
... best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV at 14-days of age and NDV GenotypeVII.1.1 at 21- days of age in broiler chickens proved that administration of Lector® and Orego® solution had positive effect on average body weight gain and feed conversion rates than control non medicated groups; where th...

Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4

...nce parameters, antibody titer against Newcastle disease virus (NDV), relative weight of lymphoid organs (bursa, spleen and thymus) and enumeration of gut microflora. Results showed that PPLP improved (P < 0.05) production performance, final body weight, carcass weight and relative weight of lymphoid organs. Anti-NDV titer was improved on both 21 (P > 0.05) and 35 (P < 0.05) day in PPLP supplemented groups as compar...

M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1

...e of BSMV had a specific titer of 1:2000. Positive reactions were obtained when purified IgG and IgG conjugate with alkaline phosphatase was 1:1000. The prepared antiserum was used detection of BSMV by serological methods i.e., Enzyme-linked immunosorbent assay (ELISA), Dot-blot and tissues-blot immunobinding assays (DBIA and TBIA). The presence of the virus was confirmed in mature seed parts and non-seed parts of the different five barley cultivars tested.

 Salwa N. Zeinl and Hanaa S. Zawam2

... of ACLSV had a specific titer of l: 8000. The concentration of IgG and IgG conjugate with alkaline phosphatase was l: 1000. The prepared antiserum was used for detection Of ACLSV using ELISA dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup).

...

Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3 

...s 22 and 34, HI antibody titer was determined against Newcastle disease (ND) and ELISA was performed for Infectious Bursal Disease (IBD). On day 35, birds were sacrificed to determine carcass yield and harvest intestinal contents for microbial and histomorphological examination. Live weights and live weight gains were higher (p = 0.05) in PB and AGP groups with the lowest feed intake in AGP (p =0.05). AGP-fed birds had better uniformity than the birds reared o...
Saiwa N. Zein
...te of TRV had a specific titer of I: 10000. The concentration of 12G and lgG conjugated with alkaline phosphatase was I : 1000. The prepared antiserum was used for detection of T RV using dot-blot immunobinding assay (DBIA). This is the first report for isolation of Tobacco rattle virus Tohrarirus (T R V) from D.Kaki under the Egyptian conditions.
...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...0.01). The highest ELISA titers against E. coli (O111), S. aureus, Str. agalactiae and Str. dysgalactiae were recorded 4 weeks post immunization in the serum and milk whey of immunized cows as compared to the non-immunized group. In cows suffering from subclinical mastitis the vaccination and /or antibiotic treatment induced significant decrease in the somatic cell counts (P<0.01). Also, significant reduction of the CFU/ml of the examined milk samples (P<...

Abdulaziz A. Alaqil

...red blood cells antibody titer, and more than 50% in T- and B-lymphocyte stimulation index, while recording a double increase in the heterophil to lymphocyte ratio. In contrast, the productive traits and immunological aspects were significantly (p < 0.05) augmented by PR supplementation to the broiler diets. Furthermore, PR supplementation successfully restored the broiler production and immune response after challenge with EC infection and elevated (p <...

Kiran Afshan1*, Sobia Baseer1, Shanza Kiran1, Ghulam Narjis2 and Sabika Firasat1

...f IgG and IgM antibodies titer was observed higher than >1.3 in pregnant women and in the 2nd and 3rd trimesters. The present study concluded a higher prevalence of T. gondii infection in women of childbearing age in KP, Pakistan, and requires preventive measures to reduce the abortion risks and congenital abnormalities in fetus.

...

Irtaza Hussain1, Muti ur Rehman Khan1*, Asim Aslam1, Masood Rabbani2 and Ahsan Anjum1

...gious ecthyma antibodies titers were found positive in sheep of Muzaffargarh only. The risk factors such as young age, female gender, grazing without stall feeding, presence of lesions on the lips, and injuries on the skin caused by prickly trees and cotton stubbles were found significantly (p<0.05) associated factors in goats; and age and injuries on the skin were important associated risk factors in sheep. The maximum occurrence of contagious ecthyma infe...

Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2

...highest humoral antibody titers throughout the vaccination schedule and conferred a complete protection against mortality with milder clinical signs, moreover displayed a significant decrease in the shedding of NDV with drastically blocked shedding 7 days PC compared to group B with little clinical protection, higher mortalities, lower antibody titers and longest viral shedding PC. In conclusion, the NDV genotype VII-based v...

Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and  Muhammad Idrees1

...and reduction of HBV DNA titer during HDV infections. Very few studies described the clampdown of HBV-related markers during HDV replication and the current study is the only report from Pakistani perspective. The objective of this study is to find the interference of HDV in HBV replication, both in HBV chronic active and inactive state patients from four different a Pakistani provinces. Blood samples along with patient history and demographic data were collec...

Saloua Ziani1*, Khalid Sohaib2 , Youssef Lhor3, Ikhlass El Berbri4, And Ouafaa Fassi Fihri4  

...r variations in antibody titers in dogs. Data from 848 dogs exported from Morocco was collected. The results revealed that too-old dogs (over six years) or too young (under four months) are also less likely to respond to vaccination than dogs that are four months to six years old. A dog receiving a booster dose is better immunized than a dog receiving a single dose. On the other hand, the time interval between the last vaccination and the time of sampling also...

Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3

... sex, breed) of antibody titers against RHDV from previous study is used for samples profiling. The results showed all samples negative for RHDV antigen with average value ±0.04 (positive cut off >0.334). Seroconversion of RHDV from all seven villages where most seroconversion are showed by samples from young rabbits compare to adult rabbits, female rabbits compare to male rabbits but insignificant among breeds group of Oryctolagus cuniculus species ...
Elamien***, Mohammed; Kotob**, Nahla S.; Elsayed*, Eltayeb A.; Ragab, Yasser M. and Mourad, Fathia E.
... and 3) samples showed a titer lower than the WHO normal limits although all samples have at least grade Il VVMs. These data indicate that type-3 poliovirus in case of bOPV needs more investigations in spite of cold chain and grade Il VVM appear to be efficient.

 
...

Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.

...0 group isolates. The HA titers of the above mentioned isolates were 6, 7, 8, 7 & 7 respectively. All the isolated Al viruses were titrated in ECE and examined for determination of their pathogenicity in specific pathogen free (SPF) 4-6 week old chicken. All the Al isolates proved to be HPAI viruses where the Intravenous Pathogenicity Index (IVPI) score for them were 2.1, 2.5, 2.3, 2.2 & 2.3 and their titers in ECE w...

Abd El-Razakl, A.G. and AboElkhair2, M.

...owing higher serological titer at the age of 35 day via ELISA test. Both two vaccines provide complete protection against mortality but not protection against bursal atrophy or histopathological changes after challenge. As well as RT-PCR-based viral IBD detection in bursa of Fabricius at day post challenge.

...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...the isolated virus had a titer 1\1024. 600 bp DNA fragments from the coat protein gene (CP) of TMV—Ch—EG was amplified with Rt-PCR technique. Phylogenetic analysis of the TMV-Ch-EG/CP- gene showed 89% nucleotide sequence homology with other published strains of TMV in GenBank and 81% amino acid sequence homology. Tissue culture approach was used to pemit the recovery of TMV-Ch-free micropropagated shoots via application of 20 mg/l virazole followed...

Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.

...thods of injections. The titer Ofthe prepared antiserum as determined by indirect ELISA with dilution of tissues 1/5 was 1/1024. Purification of the immunogamaglobulin G and conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus detection by using different se...

Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.

...a virus — specific titer of 1:7500 in bled one and 1: 7000 in bled two. Incidence of the disease reached 75% in some apiaceae crops and many of the infected plants were unfit for marketing. It is suggested that control of the disease may be achieved by introducing a celery-free period to break the cycle of virus transmission between successive crops. This work records of the first time the prescience of this disease in Egypt.

...

Zein Salwa N; Abd El-khalik, Samaa; Khatab  , Eman A.A.H and Azzam4,Clara R.

Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi

...ested samples were of HI titers of 6 log 2 oc more which we propose such titer as a protective titer against H5Nl virus. In group 2 (samples collected between April and July, 2007), titers in 36.4% of the tested samples were protective. In group 3 (samples collected between August to November, 2007).

...

Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman

... to 10-4, the peak virus titer was obtained 72 hours post inoculation, the predilection sites of the virus multiplication were the allantoic fluid, the chorioallantoic membrane and the amniotic fluid in order, where the allantoic sac route is considered the best site for virus inoculation and the virus was completely inactivated using formalin in a final concentration Of 0.1% for 18 hours at 25c0. Evaluation of the prepared vaccine batch based mainly upon vacc...

JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.

...olated viruses were then titerated and identified using different biological, serological and molecular assay.

...

Attyat, M. Kotb; Abeer, E. Mansour; Naglaa, I. Aly; Zeinab, T.Salama and Azab, A. Mohamed

...um neutralizing antibody titer expressed in log10 of FMD (strain O and strain A) in calves vaccinated with bivalent FMD vaccine only were increased from (0.9 log10 and 0.9 log10) consequently at the first week post vaccination (WPV) till it reached (2.7 log10 and 2.85 log10) for type O and type A respectively by the week. These titers were reduced to (l .2 log10 — 1.35 log10) by the 16th WPV. The FMD neutralizing antib...

Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.

...d for generation of high-titer viral stocks. In vitro expression studies utilizing the recombinant baculoviruses were conducted and revealed high-level expression of N protein as indicated by its distinct reactivity in immunofluoresence, solid phase ELISA and Western blot.

...

*Hanan M.M. Elzahed, Abd EL-Moneim A.A, *Afaf A. Khedr, *Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I.

...suring (NDV) HI antibody titers pre and post lasota vaccination and by vaccination  challenge experiment, more over no (CAV) shedding was observed in progeny chicks at early stage of breeding period. In this stage CAV shedding was recorded to be most using CAV VPI PCR assay. These results support the evidence that vaccination of breeders flock with the live attenuated (CAV) vaccine could be an effective means of control of chicken anemia virus induced cli...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...tion, where the antibody titer elevated from I st WPV till reach the protective level at 5th WPV. Effective doses in mice has been calculated in mice by inoculation of 5 groups of mice (10 each) with 2 doses of five fold diluted vaccine; inoculated mice have been challenged I/P with virulent RVF virus 103 to 104 mouse I/P lethal dose50(MIPLD50), it was ranged from 0.006 - 0.0007. Using the Rt-PCR to confirm the efficacy of binary  ethyleneamine as in...

M.A. Farag*, Abeer, E. Mansour* and s.M. 

...3%, decrease in antibody titers and low values of AOD. The immunosuppressive effects of BVDV and AFBI on the immune response of sheep vaccinated with bivalent FMD vaccine were discussed.

...

Afaf, A. Khedr*; Hyam Farouk* and Taradi, A.Said. **

...ifferences in antibodies titer in all sheep groups which vaccinated either with Rev-I and attenuated RVF alone or simultaneously.

...

S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy* 

...s. The maximum antiserum titer against either BBSV or CABMV was I :512. The dilution end point of BBSV in faba bean sap was I :320 using indirect ELISA, whereas it was 1:280 for CABMV. Both of BBSV and CABMV were detected in stems and all flower parts (sepals, petals, pistils and anthers) in intact seeds and all seed parts (seed coats, cotyledons and embryos) of immature seeds obtained from infected faba been and cowpea plants. Both of the viruses were not det...

Amal Abo El-Ela Ahmed; Eman A.H. Khatab; and M.S. Shafie

...udy had a virus specific titer or 1:2000 

...

H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1

...rked difference in virus titer when compared with those passaged in BVDV infected cells. The present study. confirms that the cytopathogenicity of the local isolates of BVDV are directly related to the presence of a helper virus which could be NCP homologous or heterologous. After 12 passages in cell culture. the obtained BVDVs in the harvests of CP BVDV infected cells may represent naturally defecting interfering viruses as a result of recombination mechanism...

Ahmed Abd El-Samie H. Ali

...ow neutralizing antibody titers as type I (3, 12, 18 and 44) and type-2 (6, 16, 20 and 48) on days 5, 7, 10 and 15 post infection, respectively. There was a significant and minor diminution in the expression of the MHC-II and MHC-I molecules respectively in virus infected calves on days 3-, 5-, 7-, and 10-days post infection when compared to control calves. There was close correlation between both in vitro and in vivo of virus immunodown regulatory effects ass...

S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3

... 46.2. which gave a mean titer neutralizing antibodies (NA) of 28.4 in their colostrums. Calves fed on these colostrums had a mean SNA of 12.2. 6.9 and 2 after one week, one month, and 2 months, respectively. In the second group (n = 7) the mean SNA in naturally intucted cows was 12.6. which gave a mean neutralizing antibodies (NA) titer of 7.7 in their colostrums. Calves fed on these colostrums had a mean SNA of 3.7, 0.9, a...

Arwa H. El -Naggar, Nirmeen G. Shafiek, and M. S. Wassel.

...luorescent. The antibody titer reach 32 by SNT and 5984 by ELISA and identified by IFA to be Bovine rota. Separation of anti B. Rota immunoglobulins IgG "were done using activated Sepharose 4B followed by conjugation with fluorescein isothiocyanate at PH 9.6. The conjugated IgG was tested against reference B. Rota antigen using direct and indirect fluorescein assay (FA. IFA). Both assay gave good result as the working dilution of the fluorescein isothiocy...

Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2* 

...ned by the antibody (AB) titers and the phytohemagglutinin-wattle reaction (PHA-WR), respectively. It was observed that most of these measures showed a linear trend response to the rise in FS levels up to 30 g/kg of broiler diets. The FS30 group presented the lowest values of CORT and MDA by approximately 21% and 15%, respectively, and the maximum levels of AB titer and PHA-WR by 38% and 3-fold, respectively. These findings ...

Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin

...derable rise in antibody titer in the third and second groups, but decreased serum antibody levels in the first group. Furthermore, the (IgG) titer in serum is measured using the Enzyme linked immunosorbent assay (ELISA) method. G1 revealed a significant decrease (P≤0.05) in the first group when compared to G2, G3, and G4. Histopathological examination of the testes revealed testicular degeneration, together with the abse...

Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil

...120 hours and infectvity titer was 7 log10 EID50/ml compared to 1.97 ICPI, 36 hours MDT, and 9.5 log10 EID50/ml of the original strain. The attenuated AOAV-1 was protective in vaccinated chicks by means of HI antibody response and protection from heterologus virulent challenge while showing little to no residual pathogencity. Histopathological inspection showed less severe pathological lesions in vaccinated challenged chickens than in non-vaccinated challenged...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
...and a specific antiserum titer (1: 3200) was obtained that had immune recognition effects for A. hydrophila and P. fluorescens. Passive immunization of Carassius auratus with Slp mouse serum and challenging with bacteria showed a passive protection rate of Slp serum against A. hydrophila infection of 42.5 % (p < 0.05) and a passive cross-protection rate against P. fluorescens of 18.6 %; the immune-related factors of LZM, AKP and ACP and the leukocyte phagoc...

Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3

...say (ELISA) and antibody titer against Newcastle disease virus (AbNDV) methods using Hemaglutination Inhibition (HI) tests. Descriptive analysis was performed on production performance data and humoral immunity to estimate phenotypic correlation of the statistical method of Pearson correlation analysis. The results of IgY examination in men aged 27 weeks were 9.49 ± 1.37 mg/mL and IgY in women 9.36 ± 2.89 mg/mL. AbNDV ages 0 and 2 weeks at 1.34&p...

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than broilers (9.38%). However, a chi-square (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chick’s house, flock size, bird health status, and practices for dead bird disposal showed no significant ...

Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4  

...for determination of the titer of the antibodies against NDV. The test results showed that the overall seroprevalence was 13.84% (n = 58/419). Layer chickens (17.62%) were more prevalent for ND than broilers (9.38%). However, a chi-square (χ2) statistical analysis revealed that factors such as the age of chickens, seasons, floor condition of the chick’s house, flock size, bird health status, and practices for dead bird disposal showed no significant ...

Minh Thi Le Bui1, Thanh Minh Le2, Thuan Khanh Nguyen1*

...t increased the antibody titer against, while chlortetracycline (10 mg/kg) in the basal diet had a low antibody titer against ND virus in chickens on 49 days of age. Feed intake and feed conversion ratio were similar among the five treatments. Weight gain was significantly different among the five treatments. Weight gain was significantly higher in T2, T3, and T5 treatments than in control treatment T1. The supplementation o...

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...period, and the antibody titers were measured using IHA test. Geometric mean titers (GMT) were computed and subsequently analyzed statistically. The results indicated that the E. coli (aggR) isolate exhibited immunogenicity in rabbits, with the antigen concentration of 10^10 cells/mL yielding highest antibody titers. Vaccines demonstrated sterility and safety, exhibiting no adverse effects...

Aya A. Saber1, Mohamed Fawzy2*, MokhtarM. EL-Tarabili2, Eman K. El Sayed2

... and ELISA. BEF antibody titers were detected by SNT in the 1st-week post first vaccination as 6 (by detecting the endpoint of the serum that inhibit completely 100 TCID50) in the group 1 and 5.2 in a group 3. Group 1 and 3 SNT showed elevated antibody titers that reached the peak (128) at the 4th-week post-second vaccination and the 3rd-month post-second vaccination respectively, Moreover, antibody ...

Quart ul Ain1, Arfan Ahmad1*, Aftab Ahmad Anjum1 and Mamoona Chaudhry2

... present study, antibody titers (geometric mean titer-GMT) showed increasing trends (48.5 to 55.5) in all vaccinated groups (p>0.05) until challenge infection followed by decrease in antibody titer (22.6 to 27.9) for 5 days PCI. This study indicated, all the vaccines found effective in reducing virus shedding and induction of humoral immunity however local vaccines presented robust redu...
Hanif Gul, M.I. Chaudhry M. Farooq and Rahmatullah Jan
...iferous timbers showed antitermitic properties at Peshawar, National Park Lalsoharna (Bahawalpur) and Changa Manga so far.

Wood extractives of Cedrud deodara (Roxb Ex. Lanb) G.Don inner and outer wood portions, Abies webbiana, Lindl., P. roxburghii.Sargent inner and outer portion and Platanus orientalis. L. heart woods were extracted with Acetone solvent in the soxhelt apparatus. The laboratory trails of the wood extractives rev...

Tarek Mahmoud Mousa-Balabel* and Karima Mohamed Abdo Abofarag

...e disease virus antibody titer and low heterophil/lymphocyte ratio in comparing with WLC. Poultry producers can use blue light color or vitamin E and Se supplementation shortly before and after the vaccination to reduce the stress of vaccination and the post vaccinal reactions in IR broilers.

...

Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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