Rehmat Ullah1*, Muhammad Zafarullah Khan1 and Kalim Ullah2
...vailability of Inputs, unviability of crop specific machinery. It was recommended that MFSC may take initiative to compel private companies to sponsor schemes for low cost inputs. MFSC may take initiative for funds on account of pragmatic trainings arrangement, subsidized inputs and crop specific machinery....
Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...ty, acrosomal integrity, viability and chromatin integrity was higher (P<0.05) in extender containing melatonin 0.1 mM and 0.5 mM compared to control. In conclusion, the addition of melatonin 0.1-0.5 mM to extender improved the post-thaw quality of buffalo bull semen....
Muhammad Aslam Shahid, Muhammad Iqbal and Muhammad Niamatullah
...ollen grain/tree, pollen viability, fruit set, yield and physico-chemical characteristics. The results indicated that males were significantly differed from one another in all these characters during both years of study. Maximum spath length (107.21, 107.81cm), weight of pollen grain (150.07, 147.57g)/ tree and number of spath/tree (18.52, 19.24) were recorded in M6, whereas, maximum spath width (15.29, 16.58cm) was observed in M5. The highest number of strand...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...2 on the post-thaw viability of cryopreserved porcine adipose-derived stem cells. Four different combinations of cryoprotectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO ...
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...atozoa such as motility, viability and plasma membrane integrity. The data was statistically analyzed with one-way analysis of variance using SPSS. The highest significant (P<0.05) response was obtained in extenders containing 0.75% green tea extract for all studied parameters. Based on the findings of the current study, it can be concluded that supplementation of green tea extract in semen extender showed significant response on the post-thawed spermatozoa...
Sitansu Pan, Surojit Khalko and Amrita Das
Effect of some fungicides on seed mycoflora, germination, viability and their persistence in treated seeds
...flora, germination, seed viability and their persistence in treated seeds of mungbean, mustard and rice was studied in vitro. The highest appearance of pathogens viz.,Alternaria alternata was recorded in seeds of mungbean (Phaseolus aureus Roxb.), A. brassicae in mustard (Brassica campestris L.) and Drechslera oryzae in rice (Oryza sativa L.). Propineb 70WP and prochloraz 45EC considerably reduced the appearance of A. alternata and flusilazole 40EC reduced Fus...
Muhammad Tariq Saeed1*, Muhammad Ashfaq Wahid1, Muhammad Farrukh Saleem1, Mumtaz Akhtar Cheema2, Muhammad Shahid1, Abdul-Shakoor1, Abdul-Sattar3
...germination and low seed viability. The optimum yield and quality of this oil seed crop is noticeably affected by its lower germination rate. To address the problem of poor germination and low seed viability, a field study was conducted at Agronomic Research Area, University of Agriculture Faisalabad, during spring 2011. The experiment was composed of two soybean varieties (Faisal Soybean and 95-1) and five priming technique...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...rmed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sperm motility, viability, morphology, membrane integrity, and acrosome integrity were decreased in treated groups. DHA concentration was increased in all supplemented groups. Whereas ALA decreased in all groups except at 5ng/ml DHA was increased line...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...e, motility, morphology, viability, and concentration. In conclusion, gradation of electrical stimulation into three stages (our modified Method II) could help to ease the collection of semen samples from bulls with minimum discomfort signs. Furthermore, the modified method is also recommended to use for other animals, in particular, the wild animals.
...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...ourse of parturition and viability of calves in Polish Black-and-White Holstein-Friesian cattle. The analysis included 1024 cows and 3663 calves of the Polish Black-and-White Holstein-Friesian breed (phf). The study included an analysis of one polymorphic place PIT-1 (c.1178G>A). The source data used to calculate the indicators of reproduction were collected from cows inseminated by semen of Black-and-White Holstein-Friesian bulls and giving birth to single...
Qingying Jia1, Hui Wang1, Tianhong Wang1, Na Dong1, Shuguang Ren1,2, Xiaolong Yang1, Jingze Liu1,* and Zhijun Yu1,3,*
...caused no effects on egg viability, whereas 4°C and prolonged 37°C and 40°C treatments on engorged H. longicornis decreased the hatchment rate of their eggs. These findings suggest that the tick H. longicornis could adapt a wide range of climate conditions and showed high tolerance to cold and low tolerance to heat.
...
Muhammad Iqbal, Jalal-ud-Din*, Muhammad Munir** and Mohibullah Khan***
...ed significantly highest viability of 95.67%. Pollination with different males on Dhakki females resulted significant variation in fruit set. The highest fruit set occurred due to M5 pollens. Fruit yield per bunch was significantly affected due to different male palms. The highest fruit yield per bunch was obtained by fertilization of M5 pollens....
Muhammad Ali*, Ghulam Mustafa Sajid**, M. Faisal Anwar Malik*** and Kalimullah****
ESTABLISHMENT OF IN VITRO CULTURE OF GRAPES
...akh-1) exhibited highest viability (100%), shoot length (4.12 cm) -1 and nodes plant (3.8). Moreover, it was found that response of cultures to different treatments was dependent both on accession and treatment duration. In conclusion, this protocol proved to be useful in optimizing the dose and duration of the treatment of grape explants with the surface disinfectant....
Rafa Almeer1,*, A. Alqarni1,2, S. Alqattan3, S. Abdi4,*, S. Alarifi1, Z.Hassan5 and A. Semlali6
...phology evaluation. Cell viability was examined by MTT assay and gene expression of three tissue inhibitors of metalloproteinase (TIMPs) and two matrix metalloproteinase (MMPs) were measured by real-time PCR. All subgroups exhibited altered morphology with accelerated detachment compared to untreated cells. The MTT assay after 48 h revealed that treatment with H1 and H2, reduced cell viability by 48% and 91% respectively, co...
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
...g/ml) could increase the viability of infected cells, and curtail the cytopathic effects of PPRV. N. sativa seed extracts should be explored in field conditions for the control of PPR.
... 
Akshay Sharma*, Mohit Mahajan, Madhumeet Singh and Pravesh Kumar
...ly interval to judge the viability of embryo (up to 42 days), fetus (after 42 days) and late embryonic mortality, if any. Sexing of the twins was done on 56th and 70th day post artificial insemination and two male twins were confirmed on the basis of genital tubercle, median raphe, scrotal and penis impression. With the aid of ultrasonography and color Doppler technique, it is possible to determine the twins’ zygosity and viabili...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...e the role of TA in cell viability and apoptosis in glioma cells (T-98G) and androgen-sensitive human prostate cancer cell line (LNCaP). In our study, 1, 5, 10, 25, 50, and 100 μM doses of TA were applied to T-98G and LNCaP cell lines for periods of 24 and 48 h. Subsequent cell vitality was tested with MTT Atest. The effective doses were analysed by flow cytometry and real-time PCR techniques. MTT spectrophotometric tests showed no significant effects on th...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
...HS could disturb growth, viability and immunity in commercial broilers. The main aim of the present study was to detect and characterize FAdVs. Liver tissues were collected from poultry flocks showing clinical signs of HHS and investigated using PCR assay for FAdVs. The FAdVs were detected in 8 samples collected from different flocks. Positive PCR amplicons were sequenced to genetically identify the FAdVs. Phylogenetic analysis revealed two distinct clusters a...
Zubaria Malik, Zahir Shah* and Muhammad Tariq
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...Progressive motility and viability of spermatozoa were significantly higher in 1.5 mM and 1.0 mM L-cysteine supplemented groups in comparison with 0.5 mM or 0 mM. The number of supra-vital hypo-osmotic swelling test positive spermatozoa at 1.5 mM and 1.0 mM was greater in comparison with lower concentration of 0.5 or 0 mM L-cysteine. Furthermore, significant improvement for acrosomal integrity was recorded in semen samples treated with 1.5 mM L-cysteine follow...
Zia-ud-Din1, Muhammad Nauman Aftab2, Tanveer Hussain3,*, Irfana Iqbal4 and Asma Zafar5
...ould be of high economic viability for such flocks.
...
Muhammad Khuram Razzaq1,2*, Saeed Rauf1, Mohsin Khurshid3, Shahid Iqbal1,4, Javaid Akhter Bhat2, Ayaz Farzand5, Adeel Riaz1,6, Guangnan Xing2 and Junyi Gai2
...n associated with pollen viability also important in improving the trait and breeding procedures to be adapted for the improvement of trait per se. Genetic studies results under heat stress in sunflower showed additive to partial dominance towards higher pollen viability in the inheritance showing that trait can be improved through selection in segregating generations. In this review, we have mainly considered the effect of ...
Sohaib Afzaal1,4*, Usman Hameed2, Nasir Ahmad3, Klas Udekwu4, Paulina Pastuszek4 and Muhammad Saleem Haider1
... LTLT treatment, maximum viability was found in the BSM-43 (42.84%) while minimum viability was shown by CYG-362 (26.10%). In case of HTST treatment maximum viability was shown by BSM-43 (41.67%) while minimally viable strain was CYG-362 (19.83%). Concludingly, Ln. mesenteroides strains tested in current study were more susceptible to LTLT pasteurization rather than HTST. Probiotic potenti...
Qaisar Jamal*, Akram Shah, Syed Basit Rasheed and Muhammad Adnan
Amit Sharma1* and Pankaj Sood2
...ing to reduced motility, viability, impaired transport and fertility. Caprine sperms are extremely sensitive to cryopreservation compared to other species which is mainly attributed to the compositional variation of the sperm plasma membrane. Different factors affect affects the semen cryopreservation viz. species, breed, semen collection technique, collection season, extender composition, type of cryoprotectant used, cooling rate, equilibration time, freezin...
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...were used to detect cell viability, flow cytometry was used to detect cell cycle and apoptosis, and immunofluorescence was used to detect Bax and Bcl-2 expression. The protein levels of TGF-β/ Smad / ERK signaling pathway were detected by Western blot. We found that PAP significantly increased cell viability after DOX-induced injury, reduced LDH, CK-MB levels, up-regulated Bcl-2 expression and down-regulated Bax express...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...han 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin acetate (Buserelin® aniMedica, Poland). After equilibration, the straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were thawed in a water bath at 38ºC for 20 sec and then were examined. Semen analysis ...
Mahak Fatima1, Memoona Syed1, Rabia Zeeshan2, Farah Rauf Shakoori3, Naveed Shahzad4, Moazzam Ali1 and Zeeshan Mutahir1*
...estern blotting and cell viability assay. As a result of siRNA transfection, MTH1 protein was knockdown to approximately 77% in the transfected sample and resulted in a 1.75-fold increase in sensitivity of MCF7-R cells to gemcitabine. Moreover, higher expression of p21 protein was also observed in transfected MCF7-R cells that may indicate induced cell death. This study highlights the effect of MTH1 gene silencing in drug-resistant cancer cells as a mea...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...lasmalemma integrity and viability were increased in extender (P<0.05) having 20.0 ng/mL of arachidic acid compared to 25.0 ng/mL, 30.0 ng/mL and control. However, sperm chromatin integrity was equally improved in experimental extenders having arachidic acid compared to control. Sperm abnormalities were reduced in experimental extender with 20 ng/mL of arachidic acid compared to other experimental extenders containing arachidic acid and control. In experime...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...ll line as a model. Cell viability was assessed by MTT assay. LDH leakage was determined using a LDH assay kit. Mitochondria membrane potential (MMP) was measured by flow cytometry. LC3 conversion and p62 expression were evaluated by western blot analysis. MTT assay revealed that spermidine decreased the viability of Hela cells in a dose-dependent manner in conjunction with disrupted morphology. Moreover, spermidine caused c...
Yaqiu Liu1, Zhijian Wang 2, Xinhui Li 1*, Jianrong Zhao2 and Weitao Chen 1
...groups. There was a high viability of P. dabryanus under the air-exposed condition (in anaerobic conditions), while the level of oxidative stress in the gill, anterior, and middle gut clearly decreased. When recovering under normoxia conditions, the physical level of oxidative stress quickly returned to normal levels.
...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...table growth factors for viability of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult bovine serum (ABS) to be evaluated as alternative to FBS. ATFs were cultured in DMEM-HG and supplemented with 10% FBS, 10% AES and 10% ABS. At passage 4, each group showed same morphology and appearance, there was also no ...
Eman A. Al-Shahari1,2, Abdelhalim A. Alkhazendar3, Eman R. ElBealy4 and Abeer A. Alm-Eldeen3*
...evel and sperm count and viability. Seminiferous tubules diameters were 84.3 ± 5.6 µm and their epithelium heights were 21.2 ± 3.4 µm. Only spermatogonia that were positively reacted with proliferating cell nuclear antigen (PCNA). After quercetin administration, they showed an improvement in the previous biochemical parameters. Seminiferous tubule diameters were 105.2 ± 2.1 µm and their epithelium heights were 26 ±...
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
...8 ±3.6%) and viability (47.5 ±2.9) percentages compared to the control group (46.68 ±2.9; 40 ±4.08%, p<0.05). All the groups with antioxidants showed decreased levels of TOS in all sonication replicates compared to the control group (p<0.05). The methionine group showed statistical differences in the TAS level measurements between the 4 and 8-repeat sonication compared to the control group (p<0.05). Upon...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...acrosomal integrity, and viability percentage were significantly higher (P < 0.05) in the diluent groups treated with 7.5 mmol of L-cysteine or 4.8 mmol of vitamin E. Correspondingly the DNA integrity and mitochondrial membrane potential percentage were found significantly higher (P < 0.05) in diluent groups treated with 7.5 mmol L-cysteine or 4.8 mmol vitamin E. The juvenile results indicated that L-cysteine and vitamin E improves the free...
Manzoor Hussain Memon1, Khalid Khan2, Anjum Parvez3, Sarfaraz Ahmed Shaikh4, Khan Mir Khan2, Guo Xiangyu5*, Mohammad Nasrullah6 and Saima Liaqat7
...d to assess the economic viability of the poultry farmers in district Mardan, Khyber Pakhtunkhwa by evaluating profitability of various actors involved in the poultry industry. The total of 200 farmers, commission agents and consumers were surveyed using multistage sampling technique. Building on quantitative industry figures the study also highlighted various qualitative problems and factors which are responsible for the production, and profitability of the p...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
... membrane integrity, and viability. Nonetheless, those with cysteine and ascorbic acid recorded better sperm morphology and acrosome integrity than those from the control group. Furthermore, the fertility of frozen semen was better with the cysteine groups than with the ascorbic acid groups. The addition of antioxidant needs further adjustment on frozen stallion semen due to changes in pH, osmolality and cryoprotectants capacity.
... 
