Ghazalah Yasmin1*, Mir Ajab Khan2, Nighat Shaheen3, Umbreen Javed Khan4
E-mail | [email protected]
Sarhad Journal of Agriculture, Vol. 31, Iss. 1
... in Pakistan using light microscopy and scanning electron microscopy. Results showed that Aconogonon is characterized by tricolpate pollen with microspinulose type of exine ornamentation. One distinctive pollen type was recognized on the basis of aperture number and sculpturing of exine under SEM. Based on analyses in the present study, diagnostic keys to the three species were provided. ...
Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2
... using scanning electron microscopy. The physical parameters such as weight and swelling degree of composite were studied. Chitosan starch based composites were used in treatment of waste water to remove a toxic anionic dye Congo red. Congo red solution (0.015 mM) was treated with polymer composite at neutral pH overnight with 150 rpm orbital shaking at room temperature. The same procedure was performed for commercial dyes. The dye was removed due to the inter...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 ceramide in promoting apoptosis. The cytotoxic effects of these components cause ultrastructural changes in A549 cells.
...
Mahanama De Zoysa1, Si-yun Ryu1, Hyeon-cheol Kim2 and Bae Keun Park1*
...ht and scanning electron microscopy.
...
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...nd transmission electron microscopy. Numerous degenerated follicles with cytoplasmic vacuolization, mitotic catastrophe in mitochondria , chromatin condensation, mitochondrial vesiculation and dispersion at ooplasm were observed. In mitochondria, mitotic catastrophe , vesiculation, swelling and loss of organization of cristae were detected.Here we showed that TiO2 exposure trigger paraptotic type cell death in zebrafish ovary.
... 
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...sed on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molecular (nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies available to virologists have obviously become more diverse and improved, the challenges have also changed greatly.Detection of plant viruses is becoming more critical as globalization of trade,particularly in hor...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8, Naimat Ullah Malik1 and Abdul Razzaq9
...nd were screened through microscopy. Results yielded an overall prevalence of 11.11%, with significant difference (P<0.05) among different study areas. Prevalence was highest in district Bannu (11.66%), followed by district Lakki Marwat and Kohat with 10% in each district. Statistical analysis revealed significant difference (P<0.05) among children of different age groups with highest disease prevalence in children of less than 5 years of age (21.40%) an...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...cidian parasites through microscopy examination. DNA was extracted from positive fecal samples for coccidian parasites and a T. gondii PCR was performed. Five sets of primers were designed using PrimerSelect tool for PCR to amplify SAG2 gene 5’ and 3’ regions. Sequences of 5 fragments of SAG2 were annotated and analyzed using DNASTAR Lasergene. After phylogenetic analysis with 3 clonal types and atypical strains, and on the basis of restrict...
Pan Hong, Ruonan Li, Yumeng Li, Hainan Lan* and Xin Zheng*
...ording to immunoelectron microscopy, showed that pGH was localized in cytoplasmic organelles, e.g., the mitochondria and Golgi apparatus. The present study shows the first exploration of the behaviour of pGH in a somatic cell model of a domestic animal (pig), providing a basis for further studies on the physiological function of pGH based on its subcellular localization.
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Bashir Ahmad1, Muhammad Idrees1,*, Kafeel Ahmad1, Dawood Ahmad2, Sajid Ali3 and Shumaila Bashir4
...rculosis by fluorescence microscopy and GeneXpert system during the study period (2015-2016) at the Provincial TB Reference Laboratory, Khyber Pakhtunkhwa, Pakistan. Among the 794 patients studied, 170 samples gave positive results. Fifty-two Mycobacterium tuberculosis complex (MTBC) isolates were randomly selected for RAPD-PCR and PCR-restriction enzyme analysis. Mycobacterium bovis (M. bovis) and M. tuberculosis H37Rv were ...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...d screened through blood microscopy. The current study revealed an overall prevalence of ovine theileriosis as 27.5%, 13% and 19.5% in districts of Dera Ismail Khan, Tank and Bannu, respectively. Sex, tick infestation, animal keeping conditions, herd size, season and feeding pattern were observed as significant factors associated with the disease occurrence. On the other hand, the effect of age and breed on the occurrence of disease was observed as non-signifi...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
... was processed for smear microscopy and culture inoculation for the detection of Mycobacterium tuberculosis infection. Moreover, the suspected samples were further investigated by polymerase chain reaction (PCR). Three samples were found positive by PCR test while other detection methods were not that sensitive to detect the tuberculosis at that stage. Two patients were reported with history of tuberculosis but were effectively treated before imp...
Muhammad Asim Khan1,*, Shahid Niaz Khan1, Sanaullah Khan2, Abdul Jabbar Khan3, Muhammad Adnan4, Nawab Ali5 and Ijaz Ali6
...e films were examined by microscopy for the detection of malarial parasites. In the Southern districts, the overall incidence of Plasmodium slides positivity was 52.47% of which 91.07% were identified as Plasmodium vivax and 6.23% as P. falciparum and 2.69 was 43.62%, of which Plasmodium vivax were 91.2%, Plasmodium falciparum were 6.09% and 2.68% were identified as mixed infections. Malaria was most prevalent in the age grou...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...> were observed by light microscopy. The germinal vesicle and the cytoplasmic organelles of the oocyte were disintegrated. The theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial cells, whereas the granulosa cells were regressed and disappeared. Erythrocytes and leukocytes were also detected at the advance stage of atresia. Apoptosis in the granulosa cells were clearly detected. The chromatin condensation against the nuclea...
Neelima Gupta
... each. Scanning electron microscopy on attachment structures revealed that the inner papillae had narrow bases and spine-like apices, the outer ones had wide bases and nipple like apices encircled by a conspicuous ring and embedded in the body wall. The body of the worm was striated and grooved assisting flexibility to the worm during movement. Attempts to develop the larvae in ducks Anas platyrynchas by feeding were unsuccessful up to 17 days post infe...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
... phoropter, slit lamp biomicroscopy, topography, keratometer and pachymetry. In this study, 50 patients of MCD were identified among which 40 were males and 10 were females, including 9 cases with family history. Main complaint was drop in visual acuity and loss of vision in all patients (100%) due to damage and accumulation ofkeratan sulfate (KS) in macular layer of corneal stroma. 87% cases have high vision loss and 13% patients have moderate vision loss. MC...
Hina Abrar1,2, S.N.H. Naqvi1,*, Muhammad Rashid Ahmed3, Asma Basharat Ali3 and Hina Yasin1,2
...ng and scanning electron microscopy (SEM) was carried at the end of dosing by using standard procedures. Serum level of ALT, ALP, γGT and bilirubin was significantly (p<0.05) increased in CBZ treated group as compared to control, whereas the hepatic parameters were significantly reduced in CBZ plus propranolol group. The histopathological examination reveals various features of hepatic architecture damage in CBZ treated group but the hepatic damage in...
Hao Gu*, Zhi Yang, Weiqiang Yu, Kan Xu and Yuan-fei Fu*
... confocal laser scanning microscopy (CLSM). In our study, capsaicin showed an outstanding performance in the inhibition of acid producing and biofilm of the four strains of cariogenic bacteria. Meanwhile, low values of MIC of capsaicin on four sectional cariogenic bacteria indicated high efficiency of antibacterial activity of capsaicin. Antibacterial activity of capsaicin on Streptococcus mutans, Actinomyces viscosus, Lactobacillus and Streptococcus sangui...
Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3
Muhammad Usman Ahmadand Ikram-ul-Haq*
...liminary on the basis of microscopy and biochemical testing. E. coli strain EC4 was screened as best oxyrase producer with activity of 0.41±0.008U/mL/min with 41% reduction in dissolved oxygen at pH 7.5, temperature 37°C, 25mM lactate as H+ donor after 20 min. This strain was later on confirmed by 16S ribotyping procedure. Oxyrase was used for improved cultivation of anaerobic bacteria by employing Hungate Technique where its antio...
Javed Asghar Tariq1*, Bashir Ahmed2, Manzoor Ali Abro2, Muhammad Ismail1, Muhammad Usman Asif1 and Raza Muhammad1
...haracterized using light microscopy and other biochemical tests. The bacteria were tentatively identified as Pseudomonas, Streptomyces, Bacillus and Micrococus. These bacteria were checked for their antagonistic activity against Fusarium oxysporum f.sp. alium cepa. They all were found to inhibit fungus showing inhibition zone 4.09 -74.97%. Hence, on the basis of these studies, it can be concluded that these bacteria can be used as a biocontrol agent to manage ...
Shamsudin Bojang1, Idris Abd Ghani2, Jugah Kadir1, Adamu Saidu Paiko1, Yasir Iftikhar3 and Muhammad Kamran3,4,*
...ected. Scanning electron microscopy (SEM) was used to identify the parasitic nematode. Both the field symptoms and SEM micrographs confirmed that the nematode isolate was M. graminis. Since this nematode has been known to damage the greens and other plants in other part of the world then the probability for the specie to adapt to other hosts other than the family of poaceae under Malaysian climate should not be discounted. Therefore, screening and restr...
Khalid P. Loneand Irfana Liaqat*
...tine histology and light microscopy. The annual maturity stages can easily be divided into five gross stages namely, immature and developing, maturing and developed, ripe and running, post spawning and spent-regressed. The testis in Labeo rohita were unrestricted and lobular. The maximum GSI (2.89±0.25) was seen in June when the photoperiod and temperatures were highest, while the lowest GSI was seen in October (0.09±0.01). The start of th...
Sadreddin Tusun*
...ime by scanning electron microscopy (SEM). The females were collected from Batman, Diyarbakır and Mardin Provinces in South Eastern Anatolian Region of Turkey and maintained in the laboratory. The different parts of the third instar larvae such as antennae, head, mandibles, abdomen and types of sensory receptors are described and illustrated.
...
Syeda Naila, Muhammad Ibrar, Fazal Hadi* and Muhammad Nauman Khan
...hloem cells. Powder drug microscopy, ash analysis, extractive values and fluorescence analysis were also studied. Preliminary phytochemical screenings of different extracts of Oxalis pes-caprae L. showed the presence of carbohydrates, phenols, flavonoids, tannins, saponin, fats and oils ...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...ht and scanning electron microscopy methods. Six chicks (6-7 weeks), six layer hens (9-13 weeks) and six studs guinea fowl were used to study and determine the histological features of the tongue of the guinea fowl. Histological structures were examined using a light microscope the tissues and papillae of the tongue were examined with a the scanning electron microscope, and photos of the general histologic structures were taken. The tongue was triangular shape...
Hina Habib Syed1, Muzafar Shah2*, Shahid Sherzada3 and Masroor Elahi Babar1
...ria was detected through microscopy of thick and thin blood smears and rapid diagnostic test of which 118 (11.24%) were found to be infected with malaria parasite. Data was also collected sfrom other labs of District Swat which accumulated to a total of 9255 patients, among which 932 (10.07%) patients were found positive for malaria parasite. Male infected were 558 (59.87%) while 374 (40.13%) were female. The collective data showed majority of the infected pat...
Muhammad Aamir1*, Riaz Muhammad1, Naseer Ahmed1, Muhammad Sadiq2, Muhammad Waqas3, Izhar1
MECHANICAL PROPERTIES OF LEAD FREE SOLDER ALLOY FOR GREEN ELECTRONICS UNDER HIGH STRAIN RATE AND THERMAL AGING
... using scanning electron microscopy (SEM) followed by confirmation
of chemical composition with energy dispersive X-ray (EDX). The microstructure is further analyzed using ImageJ
to investigate the Intermetallic compounds (IMCs) particle average size at different aging temperature. Mechanical
properties including Yield strength (YS) and Ultimate tensile strength (UTS) are examined before and after thermal
aging and at different high...
of chemical composition with energy dispersive X-ray (EDX). The microstructure is further analyzed using ImageJ
to investigate the Intermetallic compounds (IMCs) particle average size at different aging temperature. Mechanical
properties including Yield strength (YS) and Ultimate tensile strength (UTS) are examined before and after thermal
aging and at different high...
Nabeel Maqsood1,2*, Afzal Khan1, Muhammad Khalid Alamgir2, Shaukat Ali Shah1, Muhammad Fahad3
PTFE THIN FILM COATING ON 316L STAINLESS STEEL FOR CORROSION PROTECTION IN ACIDIC ENVIRONMENT
...scanning electron
microscopy (SEM) while the compositional analysis performed through energy dispersive x-ray spectroscopy (EDX).
The morphology of the coated and uncoated substrates were also studied before and after electrochemical corrosion
test and then compared. The thickness of the coating was also examined well. The result shows the remarkable
improvement in the corrosion resistance of PTFE coating by decreasing the corrosion...
microscopy (SEM) while the compositional analysis performed through energy dispersive x-ray spectroscopy (EDX).
The morphology of the coated and uncoated substrates were also studied before and after electrochemical corrosion
test and then compared. The thickness of the coating was also examined well. The result shows the remarkable
improvement in the corrosion resistance of PTFE coating by decreasing the corrosion...
Muhammad Amjad1,*, Saeed Badshah1, Muhammad Adil Khattak2, Rafi Ullah Khan1, Muhammad Mujahid1
CHARACTERIZATION OF NICKLE FREE TITANIUM ALLOY TI-27NB FOR BIOMEDICAL APPLICATIONS
...27Nb. Tensile, hardness, microscopy and scanning
electron microscope tests were conducted on TI-27Nb specimens. The tensile strength and young modulus of TI-27Nb
were lower than commonly used Titanium alloy i.e Ti-6Al-4V. The SEM results show mixed brittle-ductile surfaces.
It is concluded that TI-27Nb has lower stress shielding/bone restoration because of low modulus than commonly
used implant materials.
... 
