Robert E. Pollack
Science, Religion and Culture
...h book of daily prayers, codified in Hebrew for the most part a few hundred years ago but including some passages from the Hebrew bible’s Torah, Prophets and Writings that are millennia old.
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Souvik Ghosh, Nobumichi Kobayashi
British Journal of Virology
... VP7- and VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, providing a plethora of conclusive and vital data on th...
Jonathan Jong
...oups, obedience to moral codes, and so forth—that variously co-occur in packages we intuitively label as particular religions. Furthermore, these distinct phenomena are also present among ostensibly nonreligious (or secular) individuals and groups. Scholars of religion and nonreligion should therefore all but abandon the terms “religion” and “nonreligion”, and with them the clichéd definitional handwringing that typically c...
Ali Muhammad*, Muhammad Ayub
... lye solution which were coded as RW1, RW2 and RW3 respectively. During treatment process the bitter glucoside level such as oleourepean leached out from the fruits to the solvents by osmosis process which alters the composition of olive fruit. Phenolic compounds, flavonoids and anthocyanins which are the major components of olive fruits degrades significantly during four weeks of treatment process. Results showed that during brine treatmen...
Shubhada K Chothe, Bhushan M Jayarao and Suresh V Kuchipudi
...justify;"> Long non coding RNAs (lncRNA) contribute to a large proportion of the cellular RNA and are involved in regulating a plethora of biological and pathological processes. There is a strong evidence to suggest the association of differential regulation of non-coding RNAs (ncRNAs) to a number of disease processes including viral infections. Cellular and virus encoded lncRNAs are ...
Zuhao Huang1, Feiyun Tu2 and Dianhua Ke1*
... comprises of 13 protein-coding genes, 22 tRNA genes, two rRNA genes and two control regions CR and CCR, which was first reported in the order Coraciiformes. The overall A+T content for the mitogenome is 52%, and the GC and AT skews are -0.400 and 0.108. Unlike to many other birds, no extra base is inserted at certain position relative to ND3. Interestingly, a 484bp repeated sequence appears in both CR and CCR. Genetic distance shows that the differences betwe...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*
...ization, arrangement and codon usage analyzed. Phylogenetic Bayesian and ML analyses were conducted, using a concatenated set of 12 protein-coding genes, and adding 16 other species of gobies (Gobiidae). The mitogenome sequences of O. lacepedii,
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
...equenced. This SS cDNA encodes a protein with 114 amino acids that contains the SS14 sequence at its C-terminus. This putative peptide is identical to that generated by the SS1 gene in other vertebrates. Tissue distribution of C. nasus SS1 mRNA was analyzed by real-time polymerase chain reaction (PCR), which demonstrated high expression level in the brain. During embryogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during su...
Giselle R.R. Ayres* and Brandão P.E
... called replicase gene), code for non-structural proteins (nsps) that are enrolled in viral transcription, replication and pathogenesis. The last 3’ one-third of the genome codes the four structural and accessory proteins. Nsps act in a complex replication process, made possible by the special characteristics of AvCoV genome. This manuscript aims to present an overview of the main aspects of the current knowledge on th...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...to 24.79%. Such large barcoding gaps between intra- and inter-specific distances indicate that the COI is a suitable gene marker for molecular identification of polydorid species. Our results demonstrate that not only did all COI sequences from the larvae show greater than 99% sequence identity to those from adults, but some larvae share the same haplotypes as adults. These findings clearly indicate that the larvae collected from sea waters around an oyster fa...
Jun Cui, Xiaoxu Zhou, Zhicheng Wang, Derong Kong, Xuemei Qiu, Hongdi Wang and Xiuli Wang*
...equence is 1338bp that encodes a polypeptide of 445 amino acids. The calculated molecular weight of crucian carp Wap65 protein (CcWap65) is 50.8kDa, containing a signal peptide cleavage site between amino acids 18 and 19. The CcWap65 is a hydrophilic protein and no trans-membrane topological proteins. The SMART analysis revealed that CcWap65 contains three hemopexin-like repeats (E-value < 0.05). The crucian carp Wap65 was mainly expressed in liver, ...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
...n reading frame (ORF), encoding 164 amino acids with an estimated molecular mass of 17.6 kDa. The LvCypA has four β- strands. Tissue distribution of the LvCypA after real time analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease.
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Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
... (20 birds in each) were coded A, B, C, D, E and F; vaccinated with various combinations of IBD through different routes. Results showed that the birds of different groups received different maternal antibodies did not induce any antibodies as recorded in all groups received vaccines at days 1, 9 or 12 exhibited similar pattern antibody profiles, however, an increase in the mean titer of antibodies was recorded from 17 and onwards. Furthermore, on day 39 of ag...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... sequence of HA gene was codon optimized to the chicken biased codons and subcloned into the pCAGGS plasmid vector under the control of the chicken β-actin promoter. The in vitro expression of recombinant plasmid DNA was performed by transfection of 293T (HEK) cell line. The HA protein was analyzed using SDS-PAGE followed by Western blot and immunofluorescence assays. The pCAGG-optiH9 vaccine efficacy was evaluated by i...
P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi
Botanicals for control of anthracnose of bell peppers
...d disease incidence is recoded when compared to untreated control. In pot experiments, there is an increase in height and weight of the plants by 22% and 44% respectively in seeds treated with C. aromaticus when compared to chemical fungicide Bavistin and also 13% reduction in disease incidence is observed when compared to untreated control. From the results it is evident that C. aromaticus were effective in not only controlling the disease but also increase t...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...d in all mammals. Pit1 encodes a pituitary-specific transcription factor, which is involved in pituitary development and regulating the hormone expression in animals. In current study, Pit1 gene was screened for polymorphic sites in Sahiwal cattle. Samples from Sahiwal cattle breeds were sequenced using six set of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fr...
Ayesha Zahid,Ammara Muazzam, Sidra Mustafa, Saba Irshad*,Malik Siddique Mahmood and Rehman Shahzad
...te which was involved in coding of ASF1 like histone chaperone. Further presumption based on structural and functional analysis of mutated protein was anticipated by bioinformatics tools, which manifest mild changes in overall charge but altered post translational modifications in a way which might have a deleterious effect on ion channels anatomy and on the whole, pave ways to the genesis of cataract.
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Amir Ismail1, Muhammad Riaz1,2*, Saeed Akhtar1, Amjad Farooq3, Muhammad Arif Shahzad1 and Ahmad Mujtaba4
Muhammad Imran Shabbir
...d using ternary genotype codes to determine genotype probabilities in a very quick and simple way. Also ternary genotype codes along with gender symbols and affection status are used to modify the Punnett squares. On applying these modified Punnett squares along polygonal shapes covers all possible parental combinations and their genotypic and phenotypic outcomes in offspring. In this way, simple illustrations incorporate a ...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...onses against antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating avian influenza virus H5N1 strain in Egypt targeting the HA gene. The HA gene of AIV Egyptian strain H5N1 was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vector under the control of chicken &...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
... causative mutation. All coding exons of CTSK gene were amplified and sequenced in the affected and unaffected individuals of a consanguineous Pakistani family consisting of five members, three affected brothers, one unaffected sister and the unaffected mother. We identified a known missense mutation c.136 C>T in the third exon of CTSK changing arginine to tryptophan (p.Arg46Trp) which segregated with the disorder. The clinical findingsimplica...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
...provide effective DNA barcoding for identification.
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Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...>Control region is a non-coding region of mitochondrial genome. To infer the organization and variation of Ardeidae mitochondrial DNA control region, the control region genes of 20 species were analyzed. All the Ardeidae species had duplicate control regions, except four species having only single region. The control region spans the region between the genes for tRNAGlu - tRNAPhe in the most Ardeidae species. The length of the control reg...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...f c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both species. While expression of c-Myc gene was found to be up regulated in different canine (62.5% in mammary adenocarcinoma, 75% in oral squamous cell carcinoma, 66.6% in peri-anal sac adenocarcinoma, 80% in mast cell tumor and 100% in soft tissue sarcoma) and feline (60% in mammary adenocarcinoma and 100% in soft tissue sarcoma) tumors studied. Th...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
... In this study, a gene encoding ARM-repeat protein was identified, which has an open reading fragment of 1923 bp, encoding a predicted 641 amino acid residues with a molecular weight of approximately 71.2 kDa. BmARM-like mRNA showed highest expression in hemolymph, significantly high expression levels in 4th instar, moth, and egg, stable high expression levels in the early embryonic development, relatively ...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
... (CdGSTP1-1). The coding sequence was cloned using RT-PCR. Sequence analysis demonstrated significant differences between amino acid sequence of C. dromedarius and other mammalian GSTP1-1 enzymes. Phylogenetic relationship was studied with different organisms belonging to animal kingdom and revealed that CdGSTP1-1 is grouped with the enzyme from S. scrofa. The 3D homology model of CdGSTP1-1 showed similar fold and topology wi...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...;
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Guang-Xin E1, Yong-Ju Zhao1, Yue-Hui Ma2, Ming-Xing Chu2, Jia-Hua Zhang1, Zhong-Quan Zhao1, Hui-Jiang Gao2, Huai-Zhi Jiang3, Di Liu4, Li Liu5, Yan-Bin Zhu6, Wang-Dui Basang6, Luo-Bu Danjiu7, Tian-Wu An8, Xiao-Lin Luo8, Shi-Cheng He7 and Yong-Fu Huang1,*
...ially expressed, long noncoding RNAs (lncRNAs) found, 419 were upregulated and 607 were downregulated. The DEGs related to 56 GO categories, and pathway analysis revealed that these DEGs were significantly enriched in 41 of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including signaling pathways regulating the pluripotency of stem cells, the p53 signaling pathway, and oxidative phosphorylation. The results of the present study confirmed that t...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
... are known as small, non-coding and single stranded RNAs which can regulate cell proliferation, differentiation, apoptosis, and involve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, m...
Zahid Farooq1, Sufi Muhammad Akram2, Muhammad Saleem Khan3,* and Muhammad Wajid4
...orthern wolf snake (Lycodon striatus striatus), Sind long-nose sand snake (Lytorhynchus paradoxus)and Afghan awl-head snake(L. ridgewayi)(0.38%; N = 2) were the least abundant. Snakes were more frequent in croplands and least frequent in barren areas.
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Syed Kashif Nawaz*, Faiza Zubair and Sidra Kanwal
...her be differentiated as coding vs. coding, coding vs. non-coding and mixture of both types on the basis of overlapping gene structure. Our analyses revealed that human genome has various types of overlapping genes. The occurrence of these overlapping genes is not solely dependent on the size of the chromosomes.
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RuiHua Zhang, YongFang Jia, TingTing Liang, QianWen Yang, QiYan Du and ZhongJie Chang*
... two to four exons and encode a 780, 784 and 602 amino acid protein, respectively. Chromosome synteny analyses revealed that CcSox5 and CcSox13 weretightly linked with the etnk gene, which was conserved in all species; however, there were no conserved regions flanking CcSox6. Numerous essential transcription factor binding sites (TFs) were predicted within the 2000 bp upstream of the 5’ end of these genes. These TFs include BSX, BRN4...
Yunjun Yan, Zhenming Lü, Tianming Wang, Yongjiu Chen, Jingwen Yang, Baoying Guo, Lihua Jiang, Changwen Wu and Liqin Liu*
...3 nucleotide pairs and encodes 13 proteins, 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and a major long noncoding region (LNCR) of the mitochondrion’s own protein synthesizing system. Seven of thirteen proteins, eight tRNAs are encoded by the plus strand, while the other proteins and tRNAs, as well as two rRNAs are encoded by the minus stra...
Muhammad Luqman1,3, Raheel Saqib2*, Xu Shiwei3 and Yu Wen3
...data thus collected were coded on SPSS for analysis and interpretation. Results showed that there is significant difference in age and educational status of male heads and their spouses. Majority of the farm families (39.3%) earn income for their livelihoods both from farming and non-farming sources. Intensity of participation of female respondents in different crops and livestock activities was comparatively high as compared to their male counterparts. It was...
Yanling Xia1,2, Heping Li2, Yuntao Liang2, Jichen Zhao2, Binshan Lu2 and Di Liu1,*
...DNA sequence of the full coding region of the Vps26A gene was successfully cloned from antler tip of the Sika deer (Cervus nippon hortulorum). The Vps26A cDNA contains an open reading frame of 984bp encoding a polypeptide with 327 amino acids. The deduced molecular mass and isoelectric point of Vps26A protein were 38.2 kDa and 6.13, respectively. Glutamic acid had the largest proportion (10.4%) in the primary s...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...v>...
Hongwei Liang1,2,*, Miaomiao Tong1, Lihuan Cao1,2, Xiang Li3, Zhong Li1 and Guiwei Zou1,*
...t concentration of pharmacodynamic amino acids and the highest concentration (P < 0.05) of arachidonic acid (females: 109.83 mg/ 100g, males: 110.72 mg/100 g). Concentrations of eicosapentaenoic acid and docosahexaenoic were higher in male muscle than in female muscle. Opposite results were obtained with calipash. The n-3-to-n-6 polyunsaturated fatty acid ratios were within the recommended range in all three strains. Chinese soft-shelled turtle is a ...
Sabah Mansoor1, Muhammad Tayyab1,*, Amna Jawad1, Bushra Munir2, Sehrish Firyal1, Ali Raza Awan1, Naeem Rashid3 and Muhammad Wasim1
Ligia Neves Scuarcialupi, Laila Andreia Rodrigues Beserra, Julia Rosas Hochheim, Rodrigo Martins Soares, Fabio Gregori*
... a fragment of viral VP6-coding nucleotide sequence of each viral group. Amplicons were further submitted to genetic sequencing and analyzed by phylogenetic analysis. There were 5 RVF positive samples (5.15%) in 2 broilers, 1 laying hen and 2 breeders. Only one RVG sample (1.03%) was detected in laying hen, in coinfection with RVF. This data contributes to a better understanding of rotavirus distribution in Brazil.
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