Usman Ijaz1, Muhammad Sajid Tahir2, Khalid Abdul Majeed3*, Shahid Iqbal4, Iffat Huma5, S. Firyal6, Ijaz Ahmed7, Shahid Chohan8 and Aamir Riaz Khan9 

...ed for single nucleotide polymorphisms (SNPs). SNPs were observed on three loci of the COI gene as compared to reference sequence of Panthera pardus pardus. The specimens found in Pakistan were found to be closely related to Panthera pardus orientalis (Amur Leopard in South eastern Russia) and Panthera pardus japonensis (North Chinese Leopard), with all of these three speculated to be evolved from Panthera pardus pardus in not-so-fa...

Misbah Riaz1, Qaiser Mansoor2, Maleeha Akram1, Muhammad Ismail2, Parveen Akhtar3, Shakeel Mirza4, Mazhar Qayyum1, Afzaal Ahmed Naseem1, Faheem Tahir5 and Syed Shakeel Raza Rizvi1*

...le-stranded conformation polymorphism (SSCP) and/or sequencing. No mutation was identified in GPR54 gene, while two mutations in GNRHR gene were observed in one sporadic case of isolated HH. The first was a synonymous substitution mutation of T to G at nucleotide position 123, which did not replace valine with any other amino acid. The other mutation determined at nucleotide position 101, was a missense mutation, which substituted serine with phenylalanine at ...

Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2 

...were to identify genetic polymorphisms of the huntingtin (HTT) gene and to further investigate its association with production performances in two Anhui local chicken breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible asso...

Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim

...Cytochrome b (Cytb) gene polymorphism. Mitochondrial DNA of 24 unrelated Pakistani wild Ring-rose Parakeets was isolated and utilized for amplification and DNA sequencing of Cytb gene. The Phylogenetic analysis of the Cytb gene indicated that the Pakistani wild Rose ringed Parakeet was mono-phyletically claded with P. k. manillensis with a sequence similarity of 99.37%. Comparative analysis indicated 4 Single Nucleotide Polymorphism

Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3

...CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0.1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas in TGC were calculated as 0.365, 0.012 and 0.320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpain and Calpastatin genes and all of their genotype combinations, were also found to be carried by ...

Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1

...dants enzymes because of polymorphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A t...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...triction fragment length polymorphism (PCR-RFLP) was applied for identification of Candida species, Cryptococcus albidus, Saccharomyces, Trichosporn, Torulopsis and Rhodotorula. Using universal primers ITS1-5.8S-ITS4 amplified ITS region. And it yielded a unique PCR size of approximately 376-930 bp. PCR amplicons were digested with enzyme MspI and the generated bans corresponded to the predicted size.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...tasis. The effect of the polymorphisms in the gene have varying influences on lipid levels and development of cardiovascular disease (CVD) in different populations. In this study, we investigated the association of rs5888 polymorphism with serum lipid levels and CVD risk in an Indian population. A total of 412 samples which included 148 myocardial infarction survivors, 162 Normolipidemic healthy controls and 102 patients wit...
Jakub Jan Skorupski
...deletion and one ins/del polymorphism. The assessment of informativeness and conclusiveness of the identified variable sitesshows that the highest ability to differentiate the American mink growth hormone gene genotypes has tri-allelic polymorphism g.1219C>G (PIC = 0.3039), and three di-allelic polymorphisms - g.616G>C, g.846A>G and g.931C>T (PIC>0.2400). The recognized SNPs...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1 
...t of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fragments were aligned with the help of BLAST for SNPs identification. This is a first report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorph...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...ozygous carrier for this polymorphism. Healthy siblings of the carrier individuals and control group were screened and found as negative for this polymorphism. In-silico analysis through mutation taster, exon splicing enhancer and polyphin2 software predicted this polymorphism as a site broken and possibly damaging. These findings suggest that BDNF polymorphism
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
... gene showed a novel DNA polymorphism 37421762 (C>A) in the 3’-untranslated region (3’-UTR) of the gene. Association test was performed to analyze its relation with milk composition but it was found non-significant in all analyzed traits. So, it was inferred that it might be due to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...ed and single nucleotide polymorphisms were identified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Rabia Arif*, Shahar Bano, Muhammad Ishfaq and Muhammad Saleem
...nment while V9 showed no polymorphism. Sequences were submitted to NCBI GenBank under the accession numbers; KY001568; KY000838; KY000839 for S3, N5 and N6 strains. 1% cellulose was found effective to enhance the mycelial and perithecial growth.
...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
... association of Ala54Thr polymorphism with MetS and its risk components was studied in a local Pakistani population. MetS subjects and controls were recruited from out-patient departments of Pakistani hospitals. Ala54Thr genotyping of 200 MetS patients and 200 healthy controls was performed using a PCR-RFLP. Genotype and allele frequencies were calculated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to dete...
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...e the dependency between polymorphism in the PIT-1and fertility rates, body weight of cows and calves, the course of parturition and viability of calves in Polish Black-and-White Holstein-Friesian cattle. The analysis included 1024 cows and 3663 calves of the Polish Black-and-White Holstein-Friesian breed (phf). The study included an analysis of one polymorphic place PIT-1 (c.1178G>A). The source data used to calculate the indicators of reproduction were co...

 Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari* 

ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was observed. On the whole, 87.50% genetic identity was found in material under investigation and it ranged from 68.07% to 96.88%. The polymorphism detected among the genotypes was employed as marker for hybrid identification. Comparing the banding patterns of RAP...
Selçuk Kaplan1,* and Sertaç Atalay2
...udy was to determine the polymorphism of insulin-like growth factor 1 (IGF1) and leptin (LEP) genes in Kivircik crossbred ewes. Therefore, IGF1/BfoI and Lep/BcnI polymorphisms were examined by polymerase chain reaction- restriction fragment length polymorphism method. The single nucleotide polymorphism (SNP) in the regulatory ...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...heep worldwide. LEP gene polymorphism and associations have not been extensively reported in Lohi sheep of Pakistan, so this study aimed to find any variations in gene sequence and possible association with growth rate to explore meat potential of this breed. A total of 18 Lohi animals were selected from the flock. After the DNA extraction from blood samples, a 1486 bp fragment of exon three was amplified and sequenced. Sequence analysis revealed single nucleo...

Nadia Faqir1, Aish Muhammad2*, Ghulam Muhammad Ali2, Armghan Shehzad2, Hafeez Ur Rahman3, Farhatullah4 and Muhammad Zeeshan Hyder

...nymous single nucleotide polymorphism in Qantar, Hamin wali, Kupra and Shakri, suggesting a lack of divergence in these genes in the studied cultivars. Tree plots generated showed inconsistencies in grouping of the cultivars thus showing no significant relationship of the plant morphology with the chemical composition of fruit. 

...
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...P > 0.05). The polymorphism information content (PIC) value ranged from 0.340 to 0.921 with an average of 0.751. These markers will help the further studies, provide more useful tools to choose for researching genetic diversity and population structure and provide more in-depth understanding of Mytilus coruscus.
...

Xiangbin Meng1,Tianxiang Gao2, Chunhou Li3, Na Song1, Lu Liu1, Liqin Liu2 and Xiumei Zhang1,*

 

...llite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectively. The polymorphism information content (PIC) ranged from 0.566 to 0.823. These markers will be useful in further studies and will giv...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
...ing 54 single-nucleotide polymorphisms and 3 single-nucleotide copy number variants, and 59 haplotypes were detected. The number of haplotypes within the population ranged from 17 (SN and JL) to 28 (DX). The haplotype diversity ranged from 0.9420 (SN) to 0.9770 (NR). The highest nucleotide diversity was found in the JL population (0.01479), whereas the lowest was found in the SN (0.00894) population. Phylogenetic analysis revealed that these four populations s...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...i>Coa and spa polymorphism and association analysis indicated that spa negative isolates possess Coa of 1200 and 900bp, whereas spa positive isolates contain Coa of 650bp and 750bp. The most prevalent genes, spa, CPs8 and sea may be considered as molecular targets in designing treatment and control strategies.
...
Saba Irshad*, Aruba Muhammad, Ammara Muazzam, Farah Sarfraz Anmol and Rehman Shahzad
...ed for securitizing Xmn1 polymorphism among thalassemia (Major) patients. Out of total 206 screened patients, sole Xmn1 homozygous (+/+) and heterozygous (-/+) case was reported with a band size of 418 bp, 230 bp and 641 bp, 418 bp, 230 bp respectively. Xmn1 restriction site was present at 158 bp upstream of the Gamma globin gene on chromosome 11 of positive patients (GenBank KY927385). Fetal hemoglobin level in Xmn1 (-/+) and (+/+) was 59.1% and 19% respectiv...

Sadaf Niaz1, Masroor Ellahi Babar2, Tanveer Hussain2, Asif Nadeem1, Misbah Hussain1, Riffat Mehboob3*, Fridoon Jawad Ahmad3

Mutation analysis of RING1 domain of Parkin in early onset of ParkinsonÔÇÖs disease in Pakistani patients-a pilot study
Jun Yan Bai1,*, Yong Gang Zhao2 and Yu Qin Wang1
...n black goat, GHRL-6 has polymorphism and its mutation site is C105T. The gene frequencies of C and T at C105T in black goat were detected 0.698 and 0.302, respectively. T is the dominant allele at C105T in black goat. These research results lay a theoretical foundation for further correlation analysis with growth indexes of goats. 
...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...tase (MTHFR) gene polymorphisms and CHD and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched controls by allelic discrimination. Of 99 patients with CHD, 26 had DS. MTHFR rs1801133 and rs1801131genotypes and alleles were not significantly different between controls and C...
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...g four single-nucleotide polymorphisms and 59 parsimony-informative sites, and 18 haplotypes were detected. The number of haplotypes within populations ranged from one (WS and DJ) to six (JK). Haplotype diversity ranged from 0.000 (DJ and WS) to 0.793 (JK). The largest amount of nucleotide diversity was found in the SQ population (0.03111 ), whereas the smallest amount was in the DJ and WS (0.00000) populations. Phylogenetic analysis revealed that these 10 pop...

 Amna Younus1,3, Mariam Faiz2 and Abida Yasmeen1,*

...qI single nucleotide polymorphisms (SNPs) genotypic frequency among different clinico-pathological features in Pakistani breast cancer patients. Newly diagnosed 300 breast cancer women from different areas of Punjab visiting Institute of Nuclear Medicine and Oncology Lahore were included in this study. Different clinico-pathological features were described. Vitamin D levels were determined and VDR FokI and TaqI SNPs were analyzed. Among diffe...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
...f which 14 (40.00%) were polymorphism. The observed and expected heterozygosity in the test population ranged from 0.02 to 0.92 and from 0.02 to 0.62, respectively. High transferability rates were detected in M. butleri (37.14%), M. heymonsi (45.71%), M. pulchra (57.14%)and M. mixture (71.43%).These results indicate that the Illumina paired-end sequencing system is of great value for identifying massive numbers of genic microsatelli...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
... to assess the effect of polymorphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinical (n=50) mastitis along with non-mastitic (n=50) cows from different dairy farms of Punjab. TNF-α gene was amplified with specific primer and sequenced to get the f...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...dentification of genetic polymorphisms in genes enable us to estimate biological similarities and obtain a better perspective of quantitative traits that play key roles in development and regulating growth. The present study was conducted to characterize genetic variability in the growth hormone (GH) and growth hormone receptor (GHR) of Camelus dromedarius. GH is secreted and synthesized from somatotroph cells of the anterior pituitary lobe and plays si...
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
..., 25 primer pairs showed polymorphism among S. japonica individuals. Number of alleles, observed and expected heterozygosity per locus ranged from 2 to 8, 0.083 to 0.922 and 0.042 to 0.866, respectively. The PIC ranged from 0.04 to 0.829. These markers will be useful in evaluation germplasm and genetic composition in the further research.
...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1 
...occurrence of a T→C polymorphism in exon 5 that causes a substitution of an amino acid from Cysteine to Arginine.That Cys/Arg polymorphism may serve as authoritative genetic marker for selective improvement of growth traits in camel. These results suggest that Marecha camel has genetic variability in the IGF-1 and may be helpful for preservation strategies and future selection programs.  

 

...
Memis Ozdemir
...es about Prl/RsaI polymorphism found in the exon 3 or exon 4 region of bovine prolactin gene in the literature. The aim of this study was to determine whether the DNA sequence of the Prl gene exon 3 or exon 4 in cattle breeds has the RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in Bos taurus cattle breeds, but have been not detected ...
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
Asima Rani1,*, Syed Kashif Nawaz2 and Muhammad Arshad3
...ne the role of rs8177400 polymorphism in TIR-domain-containing-adaptor-protein gene in malaria susceptibility and clinical outcomes upon P. falciparum and P. vivax exposure. Blood samples of 228 malaria patients and 226 healthy controls were selected from the local population. Malarial samples were divided in to complicated malaria (N=89) and mild malaria (N=139) groups according to WHO criteria. Malarial groups were further divided into P. vi...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...ssion. Single nucleotide polymorphisms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, includ...
Gasem Mohammad Abu-Taweel
...necrosis of hepatocytes, polymorphism of tissue, nuclei and vessel congestions when compared with control group. The development, behavioral, biochemical and histological disorders were observed due to exposure to mercury via placenta during pregnancy and / or during lactation through weaning period. Overall, mercury chloride administration had direct influence on the social behavioural and blood parameters in the mice offsprings.
...
Jun Yan Bai*, Hong Deng Fan, You Bing Yang, Xu Wang, Heng Cao, Xue Yan Fu and Yu Qin Wang
...evelopment of sheep. The polymorphism of GHSR gene in five sheep populations was analyzed by PCR and sequencing techniques. The results showed that large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep, hu sheep all have two mutation sites (C155T and C624T). For locus C155T, allele frequencies of C in large tailed han sheep, small tailed han sheep, yuxi fatty tailed sheep, dorper sheep and hu sheep were 0.73, 0.83, 0.54, 0.69 an...
Jun Yan Bai1*, Xiao Ping Jia2, Xiao Hong Wu1, Guang Lu Li1, Heng Cao1, Xue Yan Fu1 and Kun Peng Shi1
...and eggs. In this study, polymorphism of FMO3 genes in Beijing white quail, China yellow quail and Korean quail was tested by PCR technology and hybrid DNA pool sequencing technology. Results can provide references for further exploring the action mechanism of FMO3 on fishlike smell of quail eggs. Research results show that, One SNPs mutation site (A66G) was detected from the exon 2 of FMO3, the A allele frequencies of A66G in Beijing white quail, China yellow...
Jun Yan Bai*, Hong Deng Fan, Shuai Yang, Xue Yan Fu, Heng Cao, Xiao Hong Wu, You Zhi Pang and Kun Peng Shi
Muhammad Rizwan Ashraf1, Asif Nadeem1,2, Eric Nelson Smith3, Maryam Javed1, Utpal Smart3, Tahir Yaqub4, Abu Saeed Hashmi1 and Panupong Thammachoti3
...de data was used for DNA polymorphism analyses and homology was measured among different species of genus Echis. Using the concatenated nucleotide data, Maximum likelihood and Bayesian phylogenetic trees were constructed that divided all Echis species into four groups. Saw-scaled viper in this study from Pakistan showed similarity and close relationship with Western India and UAE while showing difference from South Indian. Saw-scaled viper from South India is ...
Wenping Hu1, Xiangyu Wang1, Xiaodi Sun2, Ran Di1, Qiuyue Liu1, Zhangyuan Pan1, Xiaofei Guo1, Xiaohan Cao1, Jinyu Wang2, Yingjie Zhang3, Mei Jin4, Yuze Yang5 and Mingxing Chu1*
...on of the expression and polymorphism of TAC3 gene with the estrus of sheep. TAC3 expression in uterus and pituitary gland increased in estrus of Small Tail Han sheep and Tan sheep. The TAC3 expression level in uterus might relate with estrus of sheep. Two mutations of TAC3 were found in intron 1 (C327T) and exon 2 (G1803A) separately. Although there were significant differences of C327T/G1803A genotype distributions in five differe...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1  
...re and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA7 explained the genetic relationships of A.chukar with related members of Phasianidae family. The phylogenetic analysis also showed the genetic positioning of A. chukar with respect to other different reported species as well. The study gave us useful genomic information about genetic diversity in A. chukar and its ph...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...3 (POPA) indicating high polymorphism (PIC > 0.5). The average observed heterozygosity (Ho) and expected heterozygosity (He) in the eight populations ranged from 0.504 (POPH) to 0.767 (POPG) and from 0.6 (POPD) to 0.781 (POPA), respectively. The results showed that the eight populations possess considerable genetic diversity. The largest genetic differentiation (FST) among the eight populations was 0.233 (between POPB and POP...
Guang-Hui Tan, Yi-Yu Zhang*, Yuan-Yu Qin, Lei Wu and Jie-Zhang Li
...tigate the effect of the polymorphisms of CYP7A1 gene on lipid traits in Tianzhu Black Muscovy (Cairina moschata). We detected two novel silent mutations, CDS 216 A>G and CDS 681 T>A in exon 2 and exon 3 of CYP7A1 gene, respectively, and both SNPs changed DNA single strand conformation. The A and T allele of CDS 216 A>G and CDS 681 T>A was dominant allele, and its frequency was 0.554 and 0.800, respectively. Each SNP resulted in three genoty...

Muhammad Jawad1*, Shahid Riaz Malik1, Rana Muhammad Atif2, Haris Ahmed2 and Muhammad Shahzad Afzal3

Species Identification of Gram Wilt Complex through ITS Region by PCR-RFLP Analysis
... analysis indicates high polymorphism within the collected wilt isolates. Primers ITS 1 and ITS 4 generated internal transcribed spacer fragments that were restricted four restriction enzymes (HhaeI, MboI, BsuRI, HinfI). PCR ITS-RFLP banding pattern identified two Fusarium species i.e. Fusarium oxysporum and Fusarium solani. In conclusion, PCR-ITS-RFLP proved to be an efficient and easy technique for the identification of wilt complex pathogens.

...
Sehrish Firyal1,*, Ali Raza Awan1, Muhammad Umair Latif1, Muhammad Tayyab1, Muhammad Wasim1, Shagufta Saeed1 and Imaad Rashid2

 

...mall;">Single nucleotide polymorphism (SNPs) analysis is a powerful tool for taxonomic and phylogenetic analysis. These are bi-allelic molecular markers, easy to interpret and uniformly distributed within genomes. In this study cytochrome b (Cytb) gene based SNPs analysis was employed to genetically characterize the Pakistani domestic breed Lathy pigeon. Cytb haplotype for local domestic breed was developed. Homology analysis of Cytb gene ...
Aatif Amin1,*, Zakia Latif2, Arslan Sarwar1, Basit Zeshan1 and Mushtaq A. Saleem1
...triction fragment length polymorphism (RFLP) analysis was applied to the amplification products of 16S rRNA and merA genes and a specific restriction patterns was successfully obtained after treatment with different endonucleases. A small scale reservoir of Luria Bertani (LB) medium supplemented with 30 μg/mL of HgCl2 was designed to check the detoxification ability of the selected strains. The results demonstrated 83% detoxification of me...
Inga Kowalewska-┼üuczak1 and Ewa Czerniawska-Pi─ůtkowska2,*
...he possible influence of polymorphism in the NCF4 and SAA2 genes on selected milk and reproductive traits in the Polish Holstein-Friesian cattle (black and white variety). Analysis of genotypes of individual species was carried out using the PCR-RFLP. The frequency of the mos...
Yifan Ni1, Jian Liu1, Fen Wu1, Jianfeng Cai1, Jinzhi Zhang1*, Jianqing Hua2 and Jiping Fu2
...te the single-nucleotide polymorphisms (SNP) of the Multiple Drug Resistance Gene 1(MDR1) and the Porcine Osteopotin (OPN) gene and their association with reproductive traits in Landrace, Large Yorkshire and Jinhua sows. A total of 316 sows of three different breeds were used to examine the genotypes, and DNA sequencing method was employed to detect the potential SNPs in this study. There were two SNP loci found in these genes total...
Jun Yan Bai1*, Ren Tao Di Wu2, Qiang Zhang2, Di Bao2, Le Ma Dao2 and Xing Hua Tian3
... 0.6205, and the average polymorphism information content was 0.5602. It showed that the population of Sonid bactrian camel had high genetic diversity. Among 17 microsatellite markers, 4 microsatellites were found to be associated with body size traits of Sonid bactrian camels. The body length of AB genotype labeled LCA33 was significantly higher than that of AA genotype (P<0.05), and the chest circumference, tube circumference and body weight of AB ...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...nt-size: small;">Genetic polymorphisms in the interleukin 6 gene (IL-6) and its promoter regions have extensively been studied because of their potential role in the pathogenesis of rheumatoid arthritis (RA). Due to the confounding results, reported in several studies, we performed the systematic literature search on IL-6 -572 G/C polymorphism (rs1800796 SNP) and assessed its association with RA in the overall world p...
Zhilong Tian1, 2, Yuqin Wang2, Jishun Tang1,3 and Mingxing Chu1*
... the association between polymorphism of SYNE2 and litter size in sheep and provide a new locus for marker-assisted selection of high fecundity traits in sheep. A total of 384 small tail han sheep (STH) were sampled to detect single nucleotide polymorphism (SNP), and Sequenom Mass ARRAY®SNP assay was applied to genotype SNP loci of the SYNE2 gene. In this study, four SNPs were identified and that...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
...ion of single nucleotide polymorphisms (SNPs) of sheep THRSP gene and their association with fat deposition were investigated using Altay and White Suffolk sheep. In addition, the messenger RNA expression profiling of THRSP in fat-tailed and thin-tailed breeds was compared among 8 different tissues. Four SNPs have been detected in position g. 205A>C, c. 52C>T, c. 364A>T and c. 1031C>T of THRSP gene. Genotyping method was used t...
Jun Yan Bai*, Zhi Hao Dong, Hui Rong Gong, Xiao Ning Lu and Zi Heng Li
...gen receptor gene (ESR1) polymorphism on the carcass traits of laying quail. The polymorphism of ESR1 gene exon 4 in three laying quail populations was detected by PCR product sequencing, and the correlation between ESR1 gene and carcass traits of laying quail was analyzed. The results show that, Exon 4 of ESR1 gene among Beijing white quail, the pectora weight and pectoral muscle rate of CC genotype was significantly higher...
Jun Yan Bai*, Kun Peng Shi, Xiao Ning Lu, Xiao Hong Wu, Xue Yan Fu, Heng Cao, Hong Deng Fan, Meng Ke Chen and Yong Kang Ma

 Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*

... PCR-RFLP methods, three polymorphisms (g.1500G>A, g.1694T>A and g.2213C>G) were identified and genotyped in our population (n=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A a...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...tudy was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/HhaI gene with T allele as the dominant allele (0.61). Observed (Ho) and expected (H...

Dilara Devranoglu, Ilksen Tavaci, Gonul Filoglu and Ozlem Bulbul*

...mall;">Single nucleotide polymorphisms (SNPs) are the most abundant genetic markers in the human genome. SNPs are used in forensics for predicting the externally visible characteristics of a given individual based on a sample of DNA alone. In this study, we tested degraded DNA samples to determine the eye color prediction accuracy of the IrisPlex system. We used five years old bloodstains and UVC exposed (30 and 60 min) DNA samples. PCR and electrophoresis wer...
Yongyun Zhang1, 2, Xinyang Fan1, Fangting Zhou1, Weizhen Li3, Yina Ouyang1,4 and Yongwang Miao1*
...in dairy cattle, but the polymorphisms of the CSN1S1 gene have not been fully understood in buffalo. In this study, the polymorphisms in coding sequence (CDS) of the CSN1S1 gene for river and swamp buffalo were detected using PCR product direct sequencing. The CDS for both types of buffalo was the same in length, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 singl...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...entify single nucleotide polymorphisms and its effect on evolutionary divergence. Nine polymorphisms, six in intronic and three in exonic regions, were identified in STAT5A gene. One exonic polymorphism G→A, in exon 5, was significant that causes a non-synonymous amino acid change from Serine (S) to Asparagine (N). Further, 64 genetic variants were also identified in STAT5A<...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
...d to analyze the genetic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The results indicated that the number of effective alleles of the four Bactrian camel varieties ranged from 2.7302 to 3.0524, and average theoretical heterozygosity and average polymorphic information content were 0.6283 and 0.5546, respectively. Observatio...
Amreen Zahra1, Mushtaq A. Saleem1*, Hasnain Javed2, Muhammad Azmat Ullah Khan3, Muhammad Naveed1 and Abdul Rauf Shakoori4
Zeynep Sonmez1 and Memis Ozdemir2,*
...udy was to determine new polymorphisms in GDF5 (growth differentiation factor 5 (CDMP-1)) and EPS8 (epidermal growth factor receptor substrate 8). After the PCR analysis of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in t...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

...l16Ala single nucleotide polymorphism (SNP) is one of the most widely investigated SNPs in the MnSOD gene, where C to T transition resulting in the con-version of valine to alanine at amino acid 16 within the mitochondrial signaling se-quence. This study was designed to investigate the association of MnSOD gene SNP (Val16Ala) with HCV and HCV-related HCC to elucidate its possible role in patho-genesis of HCC. The study included sixty individuals; they were cla...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

... specific bands with 72% polymorphism, commonly bands were percentage
24%. On the other hand, the unique band appeared only in infected plants of Valor cv. with MW
85 KDa. Total phenols and proline were increased in tolerant and resistant potato cultivars
compared to susceptible cultivars and healthy ones. Scavenging enzymes (SOD, CAT, POX and
PPO) were increased mostly in tolerant and resistant cultivars compare...
Ewa Czerniawska-Pi─ůtkowska1, Iwona Szatkowska1, Daniel Zaborski2*, Wilhelm Grzesiak2, Sara Tabor-Osi┼äska1, Ma┼égorzata Wasielewska1Witold S. Proskura1, Wojciech Kruszy┼äski3 and Edward Pawlina3
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1
Xiu-Juan Zhang1 and Jin-Ping Chen1*
... 10 alleles. The average polymorphism information content (PIC) was 0.62. The observed (HO) and expected average heterozygosity (HE) value was 0.56 and 0.67 tested in 39 individual samples. For parentage testing, the allele frequency date-based indicated that the combined exclusion probability values were over 99.99% when 9 SSRs were used. This study demonstrated that the microsatellite-based approach could be effectively ut...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...o understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shangh...
Junyan Bai1*, Rentaodi Wu2*, Qihang Hu1, Lema Dao2 and Xinghua Tian3
...en microsatellite marker polymorphism and wool fineness of sunite bactrian camel was analyzed. The results show that, the average effective allele number, heterozygosity and polymorphism information content of 17 microsatellite markers in sunite bactrian camel were 2.8913, 0.6205 and 0.5602, respectively. The wool fineness of AB genotype of CMS36 locus was significantly higher than that of BB genotype (P<0.05). The...
Jun Yan Bai*, Yu Chen, Qi Hang Hu, Zhi Hao Dong, Ying Lei, You Bing Yang, Shu Juan Zhao and You Zhi Pang
...ceptor gene (ESR) polymorphism on the growth traits of quails. PCR products were used to directly sequence the polymorphism of exon 4 of ESR gene in three quail populations (Chinese yellow quail, Korean quail, Beijing white quail) and analyze the association between ESR gene and growth traits of quails. The results show that three genotypes, CC, CT and TT, were detected in exon 4 of ESR1 gene in t...

Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1

...triction fragment length polymorphism (RFLP) was performed at the same reaction conditions for all species. RFLP analysis produced species specific restriction profiles. Comparing these profiles, we have identified the species origin of meat unambiguously. 

...
Nida Sadaqat1, Abdul Wajid2, Quratul Ain2, Muhammad Zia Akbar2Farkhanda Manzoor4, Muhammad Sarwar Khan1, Nasir Ali3,  Masroor Ellahi Babar3 and Tanveer Hussain3*
...ence of PRNP gene polymorphisms. We analyzed the polymorphisms in PRNP gene sequences in 49 wild Punjab urial (Ovis vignei punjabiensis). Four novel amino acids polymorphisms (p.Q149E, p.Q155E, p.Y228L and p.L253F) were detected in PRNP gene. The urial was monomorphic at codon 149 and 155 and polymorphic at codon 228 and 253. These amino acid
Zhi Hao Dong1, Jun Yan Bai1*, Xiao Ping Jia2, Ying Lei1, Hui Cao1, You Bing Yang1 and Guang Lu Li1
...ceptor (ESR) gene polymorphism and growth traits in two populations of quail. The results showed that three genotypes, CC, CT and TT, were detected in exon 1, exon 4 and exon 8 of ESR gene. The frequencies of CC genotype in expressed exon 1 of ESR gene were the highest in french giant quail and savimit quail (0.531 and 0.778, respectively). The frequencies of TT genotype in expressed exon 4 of ESR gene were the highest in french gia...
Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1
...mall;">Single nucleotide polymorphisms (SNPs) are one of the most common and abundant class of molecular markers present in the genome of many organisms. The current study represents the first attempt to investigate the natural variations in the RK-MTases genes; Ribosomal N-lysine methyltransferase1 (RKM1) and Ribosomal N-lysine methyltransferase4 (RKM4) in Sordaria fimicola using SNP markers. A total seven SNPs in the RKM1 g...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
...ls were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and 0.27, respectively. The association of DRD2
Jaros┼éaw Pytlewski1, Ireneusz R. Antkowiak1 and Ewa Czerniawska-Pi─ůtkowska2*
...study was to analyse the polymorphism of the PIT-1 gene withe introns 4 and 5 and exon 6 in the Limousine cattle breed and its relation to selected rearing traits. Relationships were determined between the genetic variants of the three polymorphic sites of the PIT-1 gene and the following rearing parameters: body weight at birth, body weight at weaning on day 270, daily weight gain from birth to weaning, ag...
Xiaoyun He1, Yongfu La2, Jinxin Wang1, Ran Di1, Qiuyue Liu1, Xiangyu Wang1, Wenping Hu1 and Mingxing Chu1*

... the correlation between polymorphism with fecundity. The results show that it is highly possible that the promoter of FSHR gene is in the region of 2045-2295bp and 2038-2288bp, TATA box was also found there. The expression differences were found in oviduct during every stage of estrous cycle (P<0.05) between Small Tail Han (STH) sheep and Tan (T) sheep. There were significant differences in genotype distribution between STH sheep and T sheep ...

Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*

... in tail is unclear. The polymorphisms of candidate sheep CEBPA, PRKAG3 and SREBF1 genes and their relationship with fat deposition between fat-tailed (rumped) and thin-tailed breeds were investigated. Two and one SNPs were identified for PRKAG3 and SREBF1 respectively. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin-tailed breed) by Sequenom MassArray. For PRKAG3 gene, a c.1744C>T SNP and a c.184...

Shaokun Lu1,2, Ronghua Li1,2*, Chunlin Wang1,2, Changkao Mu1,2 and Weiwei Song1

...n SNP (single nucleotide polymorphism) markers were developed from EST (expressed sequence tag) database of Portunus trituberculatus. Polymorphism evaluation was tested on 30 wild individuals of P.trituberculatus collected from Xiangshan, Zhejiang province, China. The minor allele frequency ranged between 0.292 and 0.500, with an average of 0.384. The expected and observed heterozygosities (He and Ho) ranged from 0.422 to 0....

Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3

... necrosis factor α polymorphism leads to inflammation and provoke asthma which lead to morbidity and mortality in the population. The objective of the study was to look into single nucleotide polymorphism in upstream region of -238TNF-α and -308 by ARMS PCR technique in asthmatic and control group. It was a prospective study constituting 17 clinical diagnosed asthmatic patients (Diseased) and 19 non-asthmatic pat...

Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra

...rried out to explore DNA polymorphism in buffalo growth hormone gene. In this study we amplified a 5’ flanking region covering exon 1 from local specie of Bubalus bubalis. As the 5’ region of the GH is very important in controlling the expression of the gene and minor changes in this sequence can affect its expression in blood. The PCR amplicon was sequenced and analyzed for homology with the help of BLAST search. Surprisingly, along with various p...

Shakila Mumtaz1, Khalid Javed1, Muhammad Dawood1*, Muhammad Imran2, Asad Ali1 and Nazia Ramzan1

...anned to investigate the polymorphism in the beta-casein gene (CSN2) in Sahiwal (40), American Holstein Friesian (40) and the crossbred (Sahiwal × HF) (50). PCR-RFLP and conformational sequencing were performed to investigate the beta-casein polymorphism. Results of the present study showed that there was not any mutated genotype (A1A1) available in all of the three breeds. All three breeds possess dominant genotype A2...
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...nd determine whether the polymorphism that appears in the CD18 gene by the PCR-RFLP method is related to the BLAD carrier allele in Friesian Holstein crossbreed dairy cows. A total of 60 samples of cow’s whole blood were collected and extracted and DNA isolated. DNA amplification was carried out using primer Forward (BLAD_F) 5’-TCAACGTGACCTTCCGGAGG-3’ and Reverse (BLAD_R) 5’-CCCAGATTCTTGACGTTGAC-3’. Amplification of the CD18 gene ...

Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3* 

...determine how ApoB2 gene polymorphisms are related to growth-related traits and carcass characteristics of Noi chickens. A total of 183 birds were examined in the study. Body weight was recorded individually at days 1, 35, 63, and 91, and carcass parameters were determined at 91 days for all chickens. For determining single nucleotide polymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A pol...

Pan Ziyi1, Ambreen Iqbal1, Gao Zhen1, Liu Juan1, Fang Xibi2, Jiang Ping1* and Zhao Zhihui1

...e that single nucleotide polymorphisms might be used as a molecular marker for marker-assisted selection in beef cattle breeding.

...

Amira M. Nowier1, Hassan R. Darwish2, Sherif I. Ramadan3, Nadia A. Abo El-Maaty2 and Othman E. Othman2*

...s study were to identify polymorphisms in exon III of the α-lactalbumin gene among three Egyptian goat breeds (Zaraibi, Damascus and Barki) and to investigate the effect of α-lactalbumin gene polymorphism, goat breed, type of birth, season of kidding, litter size and lactation stage on body weight and milk yield traits in Egyptian goats. One hundred and sixty blood samples were collected for DNA extraction; 74 fr...

Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*

...error, single nucleotide polymorphism (SNPs) are considered to be preferable molecular markers for genetic population analysis. SNP markers that are suitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diversity and population structure of thr...

Junyan Bai*, Jingyun Li, Yu Chen, Zhihao Dong, Youbing Yang and Ying Lei

... investigate the genetic polymorphism of sheep GH gene and to analyze the correlation of genetic polymorphic sites with growth traits. The results showed that AA, AB and BB genotypes were detected in exon II of GH gene in the six sheep populations. The highest frequencies of AB genotype detected in Mongolia sheep, Small-tailed han sheep, Tong sheep, Lanzhou large-tailed sheep and Henan large-tailed Han sheep were 0.375, 0.531, 0.545, 0.350 and 0.596, respectiv...

Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1 

...ngle-strand conformation polymorphism (SSCP-PCR), and then validated by sequence analysis. The results of SSCP-PCR showed a monomorphic pattern for the β-LG gene and a polymorphic pattern for the LEP gene. The sequence analysis showed that the β-LG gene has one genotype without any single nucleotide polymorphism (SNP) while the LEP gene has two genotypes (CC and CT) with one SNP at position 194 C>T. The statisti...

Abida Shehzadi*, Muhammad Shafique and Ahmad Ali Shahid

...power of discrimination, polymorphism information content and gene diversity as 0.907, 0.854 and 0.807 respectively. Analysis of Molecular Variance (AMOVA) and multidimensional scaling plot (MDS) were also generated by using online tools at YHRD database. The data from this study could have significant importance in forensic applications, population studies and in strengthening the Y-STR database.

...

Maria Qibtia1, Sehrish Faryal1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Ahmed Mansovuri2, Zeeshan Ahmed2, Muhammad Hamid3 and Ali Raza Awan1,*

...d for the association of polymorphism. In the current study 20 out of 30 LP subjects were presented with the following SNPs: C/T-13910 (intron 13) and G/A-22018 (intron 9), representing a frequency level of 0.67 for association. Similar to this frequency of 0.22 and 0.44 was also found in LNP patients. All the SNPs were heterozygous in LP subjects, and for the particular SNP: G/A-22018 (intron 9) 22% heterozygosity was also shown in LNP subjects. This is the f...

Noora Hassan Hezam Al-Aqmer1*, Zain Aamir2, Muhammad Farooq Hanif3, Soumble Zulfiqar4, Sibgha Zulfiqar1, Mateen Izhar5 and Abdul Rauf Shakoori4*

...vitamin D receptor (VDR) polymorphism and the response to hepatitis C directly acting antiviral treatment. This study which is a case control study included 66 hepatitis C patients (genotype 3) who responded to the directly acting antiviral treatment and achieved negative HCV-RNA three months after completing the treatment (sustained virologic response (SVR)) and 66 hepatitis C patients (genotype 3) who did not achieve SVR three months after completing the sam...

Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1 

...and Random amplification polymorphism DNA (RAPD), for genotyping the Brucella isolates, as alternative rapid tools for epidemiological tracing and investigation of brucellosis in Egypt. We recovered Brucella isolates (n=29) from different host species and governorates. The isolates were identified by the bacteriological and molecular techniques (AMOS-PCR) as Brucella (B) melitensis biovar 3 (n=24) and B. abortus bv1 (n=5). The ERIC and RAPD primers (ERIC2...

Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*

...uanzhong dairy goats.The polymorphisms sites in PIS region of horned and polled Guanzhong dairy goats were identified, and a total of 30 polymorphisms sites were confirmed (A648T, T652C, G1107C, T2594C, G2820A, G3556A, A3573G, T3621C, A5329T, T5392G, G5878A, C5996T, A6187G, C6228A, G6969T, G7045T, T7057C, C7266G, C7417T, T8327G, T9029G, C10034A, A10846G, del 10861-10862TT, G11842A, G12157A, G12104A, del 12431-12432AT, G12665...

Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5

... the powerful diagnostic polymorphism detection tools at specific loci and the genome level. This review focuses on exploring the application, recent advancements and future implications of DNA-based molecular markers with the context of their role in the genetic improvement of fruit germplasm that is widely grown in Pakistan like citrus, apples, mangoes, guavas, bananas, date palms and peaches, etc. Furthermore, by offering a piece of scientific information a...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
...udy to highlight genetic polymorphism. The PCR was utilized for amplification of individual DNA samples. All of the 15 SSR markers were amplified. After gel documentation, a total number of 97 alleles were recognized having 1 to 5 polymorphic forms (OARFCB193, OARJMP29, MAF33) to 4 (OARHH47, DYMS1, SRCRSP5). For total loci, number of alleles averaged 2.1162±0.3769. Shannon’s Information index (I) 0.6184±0.2447 and the effective number of al...
Tri Eko Susilorini1*, Ahmad Furqon2, Wike Andre Septian1, Desinta Wulandari3, Suyadi Suyadi3
...med to analyse CSN3 gene polymorphism and its association with milk production and composition on Senduro goat. A total of 42 lactating Senduro goat were used in study from parity 1 to 4. Blood and milk samples were collected in Senduro district, Lumajang. The traits observed were milk yield, fat, lactose, solid non-fat, total-solid, and protein. The CSN3|Alw44I polymorphism was analysed using Polymerase Chain Reaction-Restr...

Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*

...coding single nucleotide polymorphisms (SNPs) of APOB retrieved from dbSNP using 17 different computational tools and further evaluated the structural impact of these convergent deleterious SNPs on APOB through HOPE. We found 9 missenses, 15 intronic or regulatory region SNPs and 2 were found in miRNA target sites of APOB. Out of these variant, the rs13306194 (Arg532Trp) was found in the conserved region of protein domain, which can potentially disrupt overall...

Heba Abdo Basha1, Walaa Slouma Hamouda Abd El Naby2*, Samya Erian Ibrahim3, Abeer Fikry Elnahas2 

...th the single nucleotide polymorphism in the coding region of the MSTN gene and IGF-2 (3’-UTR), using DNA sequencing. Fifty turkeys were categorized into high and low body weight. Blood samples were collected from each bird for DNA extraction. Four birds were selected randomly/group to be slaughtered for carcass traits. In MSTN, only six non-synonymous SNPs were recorded leading to four amino acid substitution in exon 1 and 2 amino acids substitution in ...
Genevieve R. Tabon1,2, Noraine P. Medina2, Mary Rose D. Uy-De Guia3, Claro N. Mingala2,3*

Ngoc Tan Nguyen1*, Minh Thanh Tram1, Thi Thu Pham1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Nguyen Khang Duong4 

...ng to analyze nucleotide polymorphism, haplotype diversity and genetic distance to construct a phylogram. Results showed that the fragment of 687 bp was successfully amplified. Examination of 49 sequence variants in 599 bp of the control region revealed the nucleotide composition was Adenine (A) = 32.55%, Thymine (T) = 28.33%, Guanine (G) = 14.67%, Cytosine (C) = 24.87% and G+C content was 39.12%. Out of a total of 50 nucleotide polymorphic sites, 48 were tran...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
...traits of sheep. Genetic polymorphism of candidate genes is associated with economic traits. Follistatin (FST) gene is one of the most notable potential candidate genes linked to economic traits. This study aimed to assess the genetic variation of the FST gene and reproductive hormones in Awassi sheep. Genomic DNA was isolated from 232 sheep (123 with a single pregnancy and 109 with twins); genotyping and sequencing were used to confirm the variants in the amp...

Muhammad Akram1*, Muhammad Imtiaz Shafiq2, Amber Malik3, Farmanullah Khan4, Munir Ahmad Bhinder5 and Muhammad Sajjad6

...ar role of GST genotypic polymorphism involved in the development of CVD. For this case-control study, a total of 504 participants including 261 CVD patients and 243 healthy individuals were enrolled after taking informed consent. The analysis of the three allelic variants GSTM1, GSTT1, and GSTP1 was carried out through PCR-based amplification. Amplification of GSTM1 and GSTT1 was performed using the specific primers designed by Primer-3 software. GSTT1 and GS...

Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5

...f CXCL9 and CXCL10 genes polymorphisms in TB patients of Pakistani population. A total of 260 study participants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with EPTB (n = 40). Tri and Tetra- ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant assoc...

Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1

...akistan for any possible polymorphism in the estrogen receptor (ESR1) gene. Blood samples in this regard were collected from clinically diagnosed eighteen breast cancer patients, all possessing invasive ductal carcinoma. DNA was amplified through PCR for 2 different sequences of ESR1 gene. Twelve normal individuals of different ages were also characterized. Amplified products were digested with Xbal and Pvull restriction enzymes and were electrophoresed to vis...
Alicja Kowalczyk1*, Inga Kowalewska-┼üuczak2, Ewa Czerniawska-Pi─ůtkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7

Xin Xing Dong1*, Gai Cui Bai2, Ming Li Li1, Guo Xiang Lan1, Da Wei Yan1, Xiao Yi Wang1, Qiang Chen1 and Shao Xiong Lu1*

... novel single nucleotide polymorphisms (SNPs) at GHRL gene in three different pig breeds, including Duroc, Yorkshire and Landrace, and the association of the novel SNPs with growth traits. PCR-SSCP and sequencing were used to detect the GHRL gene polymorphisms in the present study. Three SNPs were detected, one in the exon 1 (g.3734 G>T), which was synonymous, and two SNPs in intron 4 (g.7691 A>C and g.8523 T>C). Th...

Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana  

...entify single nucleotide polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in t...

Asma Basharat1, Abdul Wajid2*, Andleeb Batool1, Tayyeba Batool3, Abdul Basit4, Kamran Abbas5, Aziz Ullah1 and Mahmood Shaukat6

...dings suggest that TBX22 polymorphisms may responsible for a significant proportion of non-syndromic CP cases in Pakistani population and confirming its importance as a frequent cause of non-syndromic CP across various populations. The robustness of the association between TBX22 and CP is worth further examination in the future across different populations.

...

Pakistan Journal of Zoology

February

Vol. 55, Iss. 1, Pages 1-500

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