Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...rilobular hepatocellular necrosis, CD68, and Bax expressions were also increased. Treatment with all the aforementioned antioxidants downregulated most of the elevated parameters compared to the APAP-treated group. Treatment with the combination of CUR and THQ was the most effective therapies in the attenuation of liver injury assessed by a decrease in ALT and ALP activities down-regulation of Bax and CD68 expressions. It was concluded that the combinat...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ytic depletion, follicle necrosis, leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Asmatullah1, Chaman Ara1,*, Shagufta Andleeb2, Maha Tahir1, Beenish Zahid1 and Madeeha Arshad1
...cerebellum, microcardia, necrosis in atrium, underdeveloped ventricles, hydropericardium and necrosis of the liver. Skeleton analysis of cadmium treated groups showed mostly un-ossified skeletons. The control mice showed normal ossification as compared to experimental groups. Morphological, histological and skeletal studies of fetuses treated cadmium chloride along with guava fruit extract revealed ameliorative effects on va...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...e concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Furthermore, correlation analysis showed a positive relations...
Farhat Ijaz1, Rana Khurram Aftab2*, Samia Jawed3
...ukins 6 (1L-6) and tumor necrosis factor alpha (TNF-α). Relation of TNF-α with obesity induced IR and T2DM is unclear as results obtained from different studies are very controversial.
Objective: This study was designed to compare TNF-α levels and insulin resistance in obese and non-obese type 2 diabetics.
Methodology: A cross sectional comparative study was conducted in diabetic clinic of Mayo Hospital, ...
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
...of serum level of tumour necrosis factor-α (TNF-a), interleukin-6 (IL-6), lipopolysaccharide (LPS) as well as colonic mucosal tissue TLR4/9 and NF-kB. We got the following results: (1) The rat IBS-D model was successfully prepared; (2) Wumei Pill could reduce the contents of serum TNF-α, IL-6 and LPS in rats(P<0.05), and alleviate the inflammatory reaction and accumulation of bacterial endotoxins; (3) Wumei Pill could reduce the transcriptional ...
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...istological changes like necrosis, cirrhosis and dehydration in liver evident by significant elevation of Serum Glutamic Pyruvic Transaminase (SGPT), Serum Glutamic Oxaloacetic Transaminase (SGOT), Alkaline Phosphatase (ALP), total protein, bilirubin, globulin, creatine and uric acid along with reduction of SGOT/SGPT, Blood Urea Nitrogen (BUN), urea and albumin as compared to control. Treatments with JFE after Cr+6 exposures significantly improved the hepatic ...
Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3
Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...ogy
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels of hepatic enzymes AST and ALT in blood plasma of exposed fish as compared to control fish.
These results suggested that deltamethrin is highly toxic for aquatic organism like silver carp...
revealed that necrosis, nuclear pycnosis, hypertrophy of hepatocytes, vacuolization, nuclear atrophy and congestion
of blood vessels were observed as compare to control group. This result was also supported by the significant
increase in levels of hepatic enzymes AST and ALT in blood plasma of exposed fish as compared to control fish.
These results suggested that deltamethrin is highly toxic for aquatic organism like silver carp...
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...f
thymic lobules, necrosis, disappearance of lymph follicles and interfollicular edema
were the prominent histopathological changes observed in challenged group. It is
concluded that fish oil improve the growth performance and immune response in
broilers chicken against avian influenza virus infection....
thymic lobules, necrosis, disappearance of lymph follicles and interfollicular edema
were the prominent histopathological changes observed in challenged group. It is
concluded that fish oil improve the growth performance and immune response in
broilers chicken against avian influenza virus infection....
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...morphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinical (n=50) mastitis along with non-mastitic (n=50) cows from different dairy farms of Punjab. TNF-α gene was amplified with specific primer and sequenced to get the full-length sequence of this g...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...is, presence of MMCs and necrosis in hepatocytes. It can be concludedthat methylmercury has harmful effects on histology of liver of fish. Therefore, the present work suggests that the exposure to the methylmercury should be prevented to avoid injurious health hazard risks to fish.
...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...tensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolation of two B. canis strains from pet d...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...ions exhibited pyknosis, necrosis, vacuolations, increased sinusoidal spaces, karyomegaly, glomerulosclerosis, glomerulonephritis, epithelium degeneration, spermatocytes exfoliation, tubular degeneration and aspermia in liver, kidney and testis respectively. Levofloxacin causes deleterious effects on liver, kidneys and testes of mice and alters normal functioning of vital organs.
Novelty Statement | The research highlighte...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...al defects observed were necrosis, pyknosis, increased sinosuidal spaces, portal hypertension, glomerulosclerosis, abnormal vascular pole in liver and kidney respectively. But when turmeric powder was coadministered with BP, these damages were reversed to a great extent. So, this study has proved the protective role of turmeric against toxicity induced by BP in female albino mice.
Novelty Statement | Curcumin, an active co...
Sameh S. Tawfik, Ahmed A. Elkady* and Ehab T. Mohamed
...elevation in serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels when compared to irradiated rat group. The histopathological-findings of renal tissues in the irradiated-group, showed nephrotoxicity; the renal cortex showed massive necrotic changes, the convoluted tubules showed distinctive pattern of ischemic renal injury and the cuboidal epithelium cells of proximal and distal renal tubules show...
Ahsan Anjum1, Asim Aslam1, Raheela Akhtar1, Tahir Yaqub2, Muti-ur-Rehman Khan1, Rizwana Sultan3, Saba Usman1, Aneela Zameer Durrani4 and Muhammad Usman4*
...congestion, hemorrhages, necrosis and other degenerative changes were observed in tissue samples of infected cattle. Significantly higher (P<0.05) micro-pathological lesion scores were given to lungs, kidney and lymph node samples from infected cattle as compared to those of non-infected ones. In conclusion, CBPP, being a major factor affecting cattle production and associated economics, warrants the implementation of control measures to mitigate the econom...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
.... Plasma levels of tumor necrosis factor alpha (TNF-α) and alpha-fetoprotein (AFP) were estimated before and after the administration of different meals. After the experiment, the liver samples were weighed and histopathologically evaluated using a knodell score to assess portal hypertension and liver damage. Animals fed with commercial chicken meat and feed for six weeks showed development of inflammation, necrosis, a...
Gasem Mohammad Abu-Taweel
...lasm of the hepatocytes, necrosis of hepatocytes, polymorphism of tissue, nuclei and vessel congestions when compared with control group. The development, behavioral, biochemical and histological disorders were observed due to exposure to mercury via placenta during pregnancy and / or during lactation through weaning period. Overall, mercury chloride administration had direct influence on the social behavioural and blood parameters in the mice offsprings.
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...(Reg3γ), and tumor necrosis factor (TNF) were significantly downregulated. The mRNA expression levels of mucosal β-defensin, regenerating islet-derived protein 3 alpha (Reg3a), regenerating islet-derived protein 3 beta (Reg3β) and secretory immunoglobulin A (sIgA) levels were significantly increased. The interleukin-1beta (IL-1β) level was decreased. 16s RNA sequencing further indicated that the colonic microflora had changed, especially t...
Ahmad Naeem Shahzad1, Muhammad Kamran Qureshi2, Samee Ullah1, Muhammad Latif3, Shakeel Ahmad1 and Syed Asad Hussain Bukhari1*
...t stress. The absence of necrosis in cotton leaves under salt treatments indicated that growth was primarily restricted by the osmotic component of salt stress. Activities of antioxidant enzymes (superoxide dismutase, peroxidase and catalase) in plant leaves were increased in response to salt stress. Treatment of stressed plants with trehalose considerably improved the growth attributes and reduced the activities of antioxidant enzymes, indicating its ameliora...
Turgay Şişman1,*, Muhammet Çağrı Keskin1, Hatice Dane1, Şeymanur Adil1, Fatime Geyikoğlu1, Suat Çolak2 and Esra Canpolat1
...gestion, dilatation, and necrosis in proximal and distal tubules were recorded; moreover, expansions of Bowman’s space, infiltrations of lymphocytes, and glomerulonephritis were observed. Also, the frequency of nuclear abnormalities in erythrocytes showed significant differences between the sites. Quantitative analyzes of histopathological and nuclear alterations indicated that the highest effects of pollutants in frogs were at the agricultural sites in ...
Ghulam Murtaza1*, Muhammad Ramzan2, Amna Razzaq4, Muhammad Numan3, Rukhsar Beanish4, Ayesha Zafar4, Muhammad Shan Latif3 and Muhammad Adnan3
Screening of Brinjal (Solanum melongena) Varieties against Jassid, Amrasca bigutulla bigutulla
...nto leaves which results necrosis. An experimental study was conducted by using randomized complete block design (RCBD) during 2018 to check the resistant brinjal varieties (Nirala, Dilnasheen and Bemisal) against jassid (A. bigutulla bigutulla). The highest population of jassid was recorded on 5 November while lowest on 2 December on all tested varieties. The population density was lowest in December on all varieties such as Nirala (1.0), Dilnasheen (2.1) and...
Megahed, A.A.1; Kh. A.El-Dougdoug2 and B.A. Othman2
... stunting, yellowing and necrosis. Depending on the external symptoms, the present study revealed that, the total of expected and recorded viruses was five sugar beet viruses (CMV, BtMV, BNYVV, BCTV and BYV). The highly incidence percentage was recorded to BCTV (53.3 and 47.2 %) for both seasons (2009/2010 and 2010/2011). The values of ELISA conformation of total tested 240 of 915 samples of naturally cultivated plants were detected serologically by DAS-ELISA ...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
...on of phytoplasma units; necrosis starts in companion cells; severe damage in chloroplasts with abnormal membrane and thylakoid system was absent. The xylem vesicles were characterized by deforming the secondary walls. Three methods were done towards the production of phytoplasma-free tomato plantlets through tissue culture using antibiotic compound (tetracycline hydrochloride in three concentrations 25mgL-1, 50mgL-1 and 75mgL-1), irradiation by three doses (3...
Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3
...y if a tuber
necrosis-causing strain is involved.
Objective: : The purpose of this study is to characterize biologically and serologically AMV infecting
potato (Solanum tuberosum L.) in Egypt. Moreover, the study described the histological and cytological
effects of AMV infection in potato leaf cells.
Methods: Leaf samples were collected on the basis of visual symptoms from potato plants with yellow
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
...
coagulative necrosis in addition to congested blood vessels. PCR was done on the positive real-time
PCR sample, which separate amplified band at 418 bp then the sequences was submitted to the
GenBank under accession number MH260568 for the VP1 gene. Our strain has 99% identity with
nucleotides of Indian and Japan strains, 97% amino acid identity with the same strains. Our circulating
strain has low n...
Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2
...toms of leaf
necrosis, bright yellow mosaic and malformation of leaves suggested viral infection of AMV on
basil plants grown in Beni Suef governorate.
Objectives: The present study aimed to characterize the virus at the molecular level and
described the ultrastructural changes or other histopathological alterations in basil cells
following infection by AMV.
Methods: Studies were conducted...
Sajida Batool1, Saira Batool1, Sitara Shameem1, Fatima Khalid1, Tahira Batool2, Summera Yasmeen1, Saima Batool3*
...ar degeneration, diffuse necrosis, congestion and degeneration of the hepatic portal vein. Micrometric data indicated a significant reduction in themean number of hepatocytes and oval cells per unit area in ATZ treated group as well as significantly increased size and nuclear diameter of hepatocytes compared to the Cnt group. These findings showed the sensitivity of all cell types in the liver to the toxic potentials of atrazine at the given dose level.
...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...ed degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two is...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...nd increased serum tumor necrosis factor alpha (TNF-α) concentration (P <0.01), but ACTH treatment has no significant effect on them. Neither ACTH nor LPS treatment had any significant effect on levels of pulmonary SOD, CAT and MDA secretion, as well as expression of IL-6, TNF-α, IL-10, COX-2 and TLR4 mRNAs (P >0.05). Pulmonary cortisol level was inhibited by LPS (P =0.034), with a similar trend of inhibition by ACTH (...
Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3
...s wheezing sounds. Tumor necrosis factor α polymorphism leads to inflammation and provoke asthma which lead to morbidity and mortality in the population. The objective of the study was to look into single nucleotide polymorphism in upstream region of -238TNF-α and -308 by ARMS PCR technique in asthmatic and control group. It was a prospective study constituting 17 clinical diagnosed asthmatic patients (Diseased) and 19 non-asthmatic patients (Contr...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
... (NF-κB) and tumor necrosis factor α (TNF-α). The elevated levels of Creatine Kinase - MB (CK-MB), Lactate Dehydrogenase (LDH), and Troponin-1 induced by doxorubicin injection were neutralized by almond oil supplementation. Almond oil ameliorated all the histological alterations caused by doxorubicin. The administration of almond oil with doxorubicin modulated the doxorubicin-induced changes in serum and cardiac tissue in mice due to its anti...
Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3
...d. Infectious pancreatic necrosis (IPN) is caused by infectious pancreatic necrosis virus (IPNV) and it is a viral, fatal and contagious disease of young salmonids and nonsalmonid fish with acute to peracute clinical forms. This study was designed to investigate the relationship between oxidative stress and cell damage due to IPNV in rainbow trout gonad cell line-2 (RTG-2). For this purpose, IPNV was inoculated in RTG-2. The...
Rana A. Ali1, Abd-Elraheim A. Elshater2, Heba A. Mohammed3*, Mahmoud Elshazly4
...topathology, STZ induced necrosis, and degenerative changes with vacuolation. Treated groups displayed moderate to mild vacuolation and congestion of the blood vessels. Conclusion: The experience showed the effectiveness of the laser and its few side effects in treating diabetic rats.
Keywords | Diabetes mellitus, Laser, Biochemical parameters, Histopathology.
...
Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*
...crease the apoptosis and necrosis rate of prostate cancer LNCaP cells by inhibiting the expression of Akt, GSK-3β and β-catenin proteins....
De Hu, Jia Wang, Huan Wang, Xiang-Yang Leng, Tian-Yi Zhao* and Shu-Min Wang*
... and the levels of tumor necrosis factor (TNF–α) and interleukin (IL-6) in serum were measured through ELISA, and the contents of NLRP3, ASC, caspase-1 and IL-1β in gastric tissue were detected by Western blot. The results showed that FP of C. lilacina could effectively prevent the stress gastric ulcer induced by WIRS in mice by reducing the expression level of NLRP3/ASC/caspase-1....
Zhongqing Wang1, Qiuyue Wu1, Ruiling Ye1, Fazul Nabi1,4, Yangfei Shang1, Sarfaraz Ali4 and Juan Liu1,2,3*
...ithelial hyperplasia and necrosis. However, the intestinal histology of duodenal mucosa was reverted by AMP pre-treatment with normal structure and morphology with protective rate of 0 %, 70 %, 66.7 %, 40 % and 33.3 %, from IG, PG, HG, MG and LG groups respectively at 6 h. As compared to CG, the quantity of iIELs in IG was significantly (P<0.01) higher at 6 h and 4 d in duodenum and ileum of diarrheal mice; however, the iIELs quantity in IG was significantl...
Asmaa Khamis1, Osama Abdalla1, Mohamed Hashem2, Noha Abdelnaeim1*
...A, caspase-3, p53, tumor necrosis factor-α, interleukin (IL)-1β, IL-18 and, IL-10, and a significant decline in glutathione and superoxide dismutase in the CCl4 group. However, intoxicated groups treated with PN and PM showed marked improvement in the measured parameters. Therefore, PN and PM have antioxidant and anti-inflammatory effects, especially PM, which showed better improvement than PN against CCl4 hepatotoxicity in rats.
<...
Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2
...n, remarkable cirrhosis, necrosis and karyolysis was observed in live tissue of fish exposed to high Cd concentrations (0.6-1.0ppm). In conclusion, long term exposure of Cd at high concentrations showed toxic effects on intestine, kidney and liver of Rohu (Labeo rohita) and confirmed it harmful heavy metal for aquatic environments....
Rawda S. Mohamed1,2, Mohammed Y. Matoock2*, Abeer H. Abdelrazek2
...erleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), and glutathione (GSH) were measured. Mice were subjected to open field, plus maze, and dark light activity box tests. Swiss Multiple Acute concurrent Stress (SMAS) showed more responsiveness to MAS than Balb/c Multiple Acute concurrent Stress (BMAS) in most biochemical and behavioral parameters, with an elevated level of corticosterone and MDA IL-6, and TNF-α and reduced...
Rizwana Abdul Ghaffar* and Kamil Nadeem
...llular layers, fibrosis, necrosis and dilation and proliferation of blood vessels....
Peyman Mohammadzadeh1*, Aida Rasuli2, Bahman Noroozi Gorgani2, Sajjad Mohammadi3
...th hemorrhage and linear necrosis rather than only bulging. The corpus callosum and fornix had small areas of hemorrhage that indicated a sign of diffuse traumatic axonal injury. In histopathology, activated microglia and reactive astrogliosis were seen. The cause of death was TBI followed by eye blindness caused by toxoplasmosis, during which was the formation of a hematoma larger than 60 mL, a primary Glasgow coma scale less than 8, and basal nucleus ganglia...
Zhaojun Wang1, Yang Zhou2 and Xinghua Song3*
...eukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were detected using ELISA method before treatment and 3 months after treatment; incidence of adverse prognostic reactions was compared between the two groups. After treatment, the observation group had 34 markedly effective cases and 21 effective cases, with total effective rate of treatment at 91.67%. The figure was significantly higher compared to 80.00% of the control group, showing stati...
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
... were cell degeneration, necrosis, hemorrhage, congestion, and fibrosis. The findings revealed that the CD3 T cells were the most predominant inflammatory cell in hepatic mice tissue. Also, CD4 Helper T cells, CD8 Killer T cells, CD20 memory cells, and CD68 macrophages were detected in the infected mice. Most samples of mice expressed a different level of tissue necrosis where anti-caspase-3 was examined. Additionally, sever...
Mingqing Wang1 and Xiaojuan Chen2,*
...se patients. Also, tumor necrosis factor-alpha (TNF-a) was assessed as an inflammatory factor to see the response to treatment. In this study, 180 AECOPD patients who had been treated in our hospital were enrolled as research objects, who were given routine respiratory stimulant therapy regimen and intravenous methylprednisolone. According to the dosage of glucocorticoid methylprednisolone, the patients were divided into three groups, each containing 60 patien...
Ibrahim El-Ratel1*, Mostafa El-Moghazy2, Amira El-Gaml2, Ibrahim Abu El-Naser2
...normality, apoptosis and necrosis were reduced in cryopreserved semen by all PEE levels. Total antioxidants capacity was increased, while malondialdehyde was decreased in post-thawed semen by all PEE levels. Conception rate of doe rabbits was improved by artificial insemination with fresh and thawed buck semen with all PEE levels, being the highest with PEE (300 mg/buck) and slightly higher for fresh than thawed semen. In conclusion, PEE oral administration (3...
S. Feroza 1, A. G. Arijo1†, F. M. Bilqees2* and M. S. Phulan1
...lung were seen, whereas, necrosis and atrophy in infected heart were notable observations....
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
...tal area and coagulative necrosis of hepatocytes with large numbers of inflammatory cell infiltration such as eosinophil, Kupffer cell and scattered lymphocytes in the area previously migrated by young flukes as well hepatocytes displayed a fatty change with dilated hepatic sinusoids. Furthermore, our findings show that the bile ducts have hyperplasia, which is associated with fibrous cholangitis and an abundance of inflammatory cells with accumulated bile pig...
Caiping Duan
...eukin-6 (IL-6) and tumor necrosis factor-α (TNF -α) were detected by Western blotting (WB). The protein contents of B-cell lymphoma factor 2 (Bcl-2), apoptosis-related genes Bax and caspase-3 were detected by WB. The contents of lactic dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Results showed that compared with the control group, the low-dose pr...
Characterization and Molecular Detection of Tomato spotted wilt Tospovirus Infecting Tomato in Egypt
Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2
...mptoms varied from local necrosis, systemic necrosis, systemic mosaic yellowing, bronzing, or stunting. The virus transmitted by mechanical means as well as by Thrips tabaci. Its TIP was between 45-50oC, DEP was 10-5 and LIV was between 5-6 hrs. Local lesion technique for biological purification and electron microscopic analysis confirmed the production of mature infectious virus particles underlining the conclusion that a f...
A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1
...ed local lesions and top necrosis on Capsicum annuum L. cvs. California Wonder & Godion as well as Lycopersicon esculentum Mill. cvs. Streen B and Casel Rock. Solanum tuherosum L cv. Cara. Nicotiana glutinosa L. Nicotiana benthamiana gave systemic infection only. On the other hand. systemic symptomless infection had occurred with N. tabacum L. cvs. White Burley and Xanthine. Whereas PAMV didn't infect Ch. umaranticolor Cost. Reyn and D. metel L. The virus ...
Rahma Boukarine*, Leila Hamdi and Kamel Khelili
..., hemorrhage, hepatocyte necrosis and glomerular degeneration, the effect of ESAE was effective in modified these damages into semi normal.To conclude, xylene administration induced hepatotoxicity and nephrotoxicity in wistar rats, while the combination of this solvent with rocket aqueous extract (Eruca sativa) attenuated this toxicity thanks to its antioxidant property....
Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2*
...Severe damage comprising necrosis of the hepatic cells, renal tubules, congestion, and infiltration of inflammatory cells was seen in the chemotherapy-treated group and mice suffering from cancer without treatment. This study also revealed variations in serum liver enzyme values between treatment groups. The values of ALT, AST, and ALP (U/L) evaluation in control groups were ˃ 29.01±1.8, 87.55±2.9 and ˃ 98.12±8.8, respectively. In conclu...
Munir Ahmad1, Abdul Ghaffar1, Riaz Hussain2* and Rifat Ullah Khan3*
...Bowman’s space and necrosis in kidneys. Different histopathological lesions in liver of treated test specimens including edema, hemorrhages, atrophy of hepatocyte and hepatocyte having eccentric nuclei. In the brain, different histological alterations like necrosis and degeneration of neurons, and microgliosis were seen, whereas the heart of treated fish showed hemorrhages, edema, and neutrophilic myocarditis. In concl...
Lianci He1, Dingxi Bai2, Chenxi Wu2, Yizhu Zhong2, Shi Chen2, Xinru Bao2, Jing Gao2*, Chaoming Hou2*
...eta (IL-1β), tumour necrosis factor alpha (TNF-α), transforming growth factor β-activated kinase 1 (TAK1), TAB, IκB kinase (IKKα), IκBα, NF-κBp65 and cyclooxygenase-2 in the serum were measured. Compared with the CON group, the CFS group had decreased weight and decreased activity and mobility (P < 0.05). Compared with the CFS group, the three SXC groups and the FLU group all had varying levels of increased body...
Khalid M. Al-Syaad1,2
...cluding atrophy, tubular necrosis, enlargement of the glomeruli, dilatation and congestion of the glomerular capillaries (G) and the blood vessels around the renal tubules, atrophy in some renal glomeruli, hemorrhage, and around edema interstitial the blood vessels. It is concluded that utilizing propolis with CC considerably reduced the biochemical and histological damage produced by CC, and it is suggested that propolis could be utilized as a cytoprotective ...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...ptosis, early apoptosis, necrosis, and malondialdehyde level; most favorable results were recorded with PEE2. The foregoing results indicated that supplementation of Tris-semen extender with propolis ethanolic extender at a level of 8 mg/ml increased the functional activity of spermatozoa, decreased sperm apoptosis, and necrosis, and improved the antioxidant status of sperm medium in cryopreserved semen of Egyptian buffaloes...
Weijun Chen1*, Shiyang Gao1, Ping Sun1, Xiaohui Sun2, Yongbin Wei1, Jingyang Chen1 and Jialong Chen1
...mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in liver of common carp compared to control diet (P<0.05). However, diet NaBT significantly improved fish liver health by decreasing contents of TCHO and MDA, down-regulating mRNA expression of pro-inflammatory cytokines (e.g., TNF-α, IL-1β and IL-6), increasing the activity levels of antioxidant enzymes, and upregulating the mRNA expression...
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
...d. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH) and reduced glutathione peroxidase (GSH-Px) were detected by ELISA. The expression level of TLR4/NF-κB, and HandE staining was used to observe the renal tissue. In the results, compared with model group, the spleen index and whole blood viscosity was significantly decreased...
Azza M. El-Kattawy1, Tarek Abou Zed1, Randa Megahed1 and Mohammed El-Magd2*
...ion-related genes [tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL1β)], 3) declined the expression of matrix metalloproteinase-3 (MMP3), transforming growth factor beta (TGFβ), and oxidized-LDL receptor (OLR1), 4) upregulated the expression of the anti-inflammatory gene IL10, 5) reduced the levels of the oxidative markers [lipid peroxide marker malondialdehyde (MDA) and nitric oxide (NO)], 6) increased the levels of antioxidant ma...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
..., that results in bursal necrosis and severe damage of the immune system. IBDV remarkably persist to affect the poultry industry in Egypt. Hence this study was done to identify and characterize very virulent Infectious Bursal Disease virus in Egypt during the period 2019-2020. bursal samples were collected from 8 vaccinated broiler flocks exhibited high mortality rates and bursal lesions in al-El-Fayoum province, Egypt during 2019 and 2020. Reverse transcripti...
Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*
...us infected with nervous necrosis virus (NNV), this research initially explored the main genes and key pathways related to anti-NNV. This study used the Illumina HiSeq 4000 sequencing platform to sequence the transcriptome of E. fuscoguttatus, the transcriptome data clustered by TGICL to obtain Unigene, and bioinformatics methods were then adopted to perform functional annotation classification, metabolic pathways and SSR feature analysis. The results showed t...
Fariha Qahar and Muhammad Sayyar Khan*
...lines showed only 15-20% necrosis. The data suggested that overexpression of NtSAT4 is a promising strategy for improved stress tolerance against these xenobiotics in B. napus....
Ihab G. AL-Shemmari1*, Ali Hussein Fadhil1, Mohammed Assad S. Alkabi1, Eman Jawad Jabber2
...l alterations, including necrosis, mononuclear cell infiltrations (MNC), calcification, fibrosis, and severely constricted blood vessels. Conclusion. The current study concluded that detecting brucellosis in ewes using a combination of molecular techniques and culture yielded the highest reliable results, suggesting that this method might be employed as a rapid regular screening test. ...
Nusrat Habiullah1*, Shah Nawaz Kumbar1, Fahmida Parveen Samo1, Shamusuddin Bughio2, Asghar Ali Kamboh3, Burirah Rehman Talpur1
...d bowman’s spaces, necrosis and inflammation. Whereas, liver shows severe dilation of central vein and sinusoids, congestion and necrosis. Group E showed no major changes in liver and kidney while group F has less severity than groups C and D. The present study concluded that selenium has a protective effect against toxicity produced by lead acetate as evident from reduced morbidity, amelioration of liver and kidney fu...
Haneen Imad Al-Sultani1*, Ahmed Obaid Hussain1, Hazar Shakir Saleh2
...te, and severe injuries: necrosis, congestion, hemorrhage, glomerular degeneration, fatty changes, cysts and vacuoles in the renal tubule cells and lymphocytic infiltration in the kidney tissue with gradual increase in rosuvastatin concentrations 10, 20 and 40 mg/kg, respectively. Conclusion: It was concluded in the current study that the use of rosuvastatin in high doses causes nephrotoxicity. ...
Jasir Hakim Hidayah1,2, Yos Adi Prakoso2, Sitarina Widyarini3*
...stopathological score of necrosis neuronal, microgliosis, neuronal oedema, secondary haemorrhage, and perivascular oedema compared to the others. This study proved that calabash can be an alternative therapy against ischemic stroke. In advance, the experimental intoxication of this fruit and its processing product must be elucidated. ...
Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2
...-c concentrations, tumor necrosis factor-alpha (TNF-), and interleukin 10 (IL-10) concentrations. The results revealed the development of signs of incitement such as increased TC and reduced IL-10, decreased HDL-C, and increased TC, TAG, VLDL-C, and LDL-C, along with an increase in inflammatory markers. CPNPs reduced dyslipidemia and inflammation in female rats. We implied the protective impact of CPNPs, which may be seen in the anti-inflammatory and hypolipid...
Rasema Majeed, Alaa Kamil Mahmood*
...t showed destruction and necrosis of islet cells, congestion and edema between their acini. Third group was administered 81.7 mg/kg BW of ginger ethanolic extract orally for 45 days showed normal section of islet cells shows moderate necrosis and destructions of islet cells. congested blood vessels, regeneration of islet cells. fourth group was administered 81.7 mg/kg BW of ginger ethanolic extract loaded with chitosan nanop...
Ahmed, Amal A. and Fath-Allah, Mervat M.
... mottling, marginal leaf necrosis deformation and abortive flowers grown at Kalubia and el-Fayoum Governorates, Egypt. The identities of the two viruses were confirmed serologically using DASELISA. Graft inoculation of diseased apricot scion onto healthy one showed the same symptoms as those seen in naturally infected trees in the stock shoots. Similar symptoms were caused by slashing inoculation with surgical knife onto the stem of young healthy apricot seedl...
Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*
...actor), and TRAF6 (Tumor necrosis factor receptor-associated factor 6) in chicken erythrocytes infected with M. synoviae using quantitative real-time PCR (qRT-PCR) at four different time intervals (0, 2, 6 and 10 h) post-infection and compared to uninfected controls. The results indicated that M.synoviae interacted efficiently in chicken erythrocytes, which strongly induced the up-regulation of NF-κB pathway and other immune system genes in response to e...
Anisa Muthia Fakhira1, Madihah Madihah1, Susi Susanah2, Erick Khristian3, Ratu Safitri1*
...udy showed that both the necrosis score in pancreatic beta cells and fasting blood glucose levels demonstrated a dose-response pattern, with higher values corresponding to increasing dosages. The ID dose starting from 20 mg/kg BW showed a significant increase in pancreatic beta cell necrosis of 1.6 ± 0.55 and a fasting blood glucose level of 167.40±11.45 mg/dL, classifying it as a prediabetic state, that signif...
Qamer J. Jadoaa, Raffal A. Omar*
...aline to prevent thermal necrosis. The experimental animals were divided randomly into three equal groups, each group include fifteen rabbits (n=15). control without any additive. group 1(lidocaine hcl). Which applied daily single dose of 2% lidocaine Hcl 2 mg/Kg. B. W. for five days post-operation (P. O.), while the group 2(diclofenac) applied 3.75% of 20mg/Kg. B. W. histopathological specimens were taken at the end of the 7th,14th, and 21th day p.o. The resu...
Tayfun Karatas1*, Betul Apaydın Yıldırım2 and Serkan Yıldırım3
...neration, steatosis, and necrosis in liver hepatocytes as well as hyperemia and inflammation in the liver and caused degeneration, desquamation, necrosis and adhesion in the gill epithelium. Additionally, expression levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) and Caspase 3 were severe in the liver and gill tissues. However, both 50 and 100 mg doses of TLE had protective effects against CMN-induced toxicity in all the above...
Hongsen Xu*, Xiaoni Wang, Tie Tian, Changyu Zhao, Denghang Yu and Jun Liu
...as cell degeneration and necrosis, hemorrhage and inflammatory cell infiltration, were found in diseased crayfish. In conclusion, this study confirmed the pathogen of whitish muscle disease in farmed P. clarkii, and preliminarily investigated the biological characteristic, pathogenicity and drug sensitivity of this bacterium. The results of this study will provide scientific guidance for the effective prevention and control of muscle turbidity of P. clarkii.
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...the expressions of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in the pulmonary section of mice induced with canine coronavirus (CCoV) vaccine. The research utilized male mice (Mus musculus) aged two to three months, divided into three groups: a control group administered with normal saline (D0), a CCoV-induced group (D1), and a group induced with CCoV along with 1 mL/kg body weight of Lactobacillus acidophilus for seven days ...
Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee
...neralized hepatocellular necrosis, which revealed congestion, hemorrhage, and degeneration of hepatic cells. Some sections of the kidneys show vacuolation of mesangial cells in the glomerular tuft. Muscles of treated rats revealed severe histopathological changes under stress from both doses of toxic cholecalciferol, also showing the widespread mineralization of muscle fibers. From this study concluded that both doses of vitamin D can be toxic to organisms at ...
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
...e (3), whereas the tumor necrosis factor alpha (TNF α) expression showed highest score (4) in the testes and epididymis tissue. The conclusion of the recent investigation found that tetrodotoxin has a harmful toxic effect on rats that indicated the effects of oxidative stress and increase interleukin1 (IL 1) and tumor necrosis factor alpha (TNF α) expression also induce hypospermatogenisis in testes and sperm sta...
SHAGUFTA ANDLEEB1*, ASMATULLAH2, SHABANA SHAUKAT1 & SIDRA NAWAZ2
...ormations like pyknosis, necrosis, and steatosis....
NOREEN RAZA1*, TASNEEM A. SAQIB2, SYED NAEEMUL HASSAN NAQVI3, MUHAMMAD ZAHEER
KHAN2 & MAZHAR HIJAZI3
...rophy along with hepatic necrosis; reduction in thickness of muscular layer of gizzard and marked alterations in loop of Henle of kidney. While, low concentration of Lead nitrate showed slight alteration in loop of Henle in kidney; disruption and thinning of epithelium of gizzard and mild disruption of hepatic cords in liver. No laceration was observed in the mentioned body tissues of Larus argentatus of control group....
ADEEBA SYED1, DILAWAR HUSSAIN2, UZMA RAFI1 & SUMAIRA MAZHAR1*
...istological changes like necrosis, infiltration, atrophy, shrinkage and degeneration of intestine was observed for different CPF concentrations. Kidney sections of Labeo rohita under different CPF concentrations exhibited nuclear hypertrophy, vacuolar degeneration of glomeruli, and occlusion of tubular lumen, cloudy swelling degeneration and hyaline droplets degeneration. In the liver tissue prominent histological changes observed including hepatic cell degene...
UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1
...t-align: justify;">Tumor necrosis factor alpha is known as multifunctional cytokine component of apoptosis and cell survival. Preterm delivery is a confused prenatal unfavorable occasion. This examination work was planned to associate relationship of G308A and -238 polymorphisms in Tumor necrosis factor alpha. We collected blood samples of 70 patients out of which 50 ladies gave untimely births and 20 ladies gave typical bir...
Aliya Irshad Sani1, Shumaila Usman2*, Zile Rubab1, Sufyan Ahmed3, Sana Iqbal3 and Zehra Ahmed4
...ry molecules OX40 (Tumor necrosis factor receptor superfamily, member 4, TNFSRF4) and OX40L (tumor necrosis factor superfamily, member 4; TNFSF4). They function to potentiate T cells function to accentuate anti-tumor activity. The results of OX40 and OX40L in cancer immunotherapy are still limited and most of the studies have evaluated their expression in tumor biopsies. In this study we aimed to find out gene expression of ...
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
... Various form of nuclear necrosis with number apoptotic hepatocyte, nuclear pyknosis with evidence of councilman body in liver tissue, The Immunohistochemistry study revealed an increase in the scoring of IL1 expression in liver that showed score (3) compared to control group, TNF alpha expression showed score (1) in liver tissue when compared to untreated control group . According to the findings of the current study, tetrodotoxin has adverse toxic effects on...
Abeer Alahmari1,2*
...ding shrunken glomeruli, necrosis, inflammatory cell infiltration, congestion, and edema. They also exhibited significantly elevated lipid peroxidation and oxidant radicals with decreased antioxidant parameters in renal tissue. In contrast, AME alone did not cause renal injury, and it was able to notably improve the toxic impacts of ethanol in the kidney when administered together. In conclusion, AME provided preservation against ethanol toxicity by scavenging...
Marah Salim Hameed1, Ali Ibrahim Ali Al-Ezzy2*
...luted tubules and tubule necrosis with dilation and vacuolar degeneration of proximal and distal convoluted tubules. Nitro blue tetrazolium activity for monocytes and macrophages do not correlate with blood urea and creatinine concentrations. Vitamin E -sodium selenite and vitamin C have important role in enhancement for renal functional activity and clearance of urea and creatinine; They considered potent antioxidant that ameliorates the histopathological eff...
Hayder S. Rwayyih*, Tahani S.S. Al-Azawi
...d aorta. There was clear necrosis of cardiac muscle fibers with fatty change in endocardium or papillary muscles due to estrogen deficiency, irregular myocardial endothelial degeneration and congested blood vessels. The aorta of these groups has enlargement and thickening of endothelial cells with infiltration of fatty cells in tunica intimae (The structure of intima changes in postmenopausal and it becomes multilayered due to migration of smooth muscle cells ...
