Muhammad Nawaz Kandhro*, Habib-Ur-Rehman Memon, Muhammad Ali Ansari, Ahmed Naqi Shah
...orted to contain several allelochemicals. Utilization of these allelochemicals is considered low-cost and natural sources for weed suppression. Field trials were undertaken at Cotton Section, Agriculture Research Institute, Tandojam Pakistan during summer 2010 and repeated during summer 2011 for validating the previous results. The experiments were laid out in three replicated randomized complete block de...
Qaizar Ahmed1, Fida Mohammad2, Hidayat-ur-Rahman2, Sheraz Ahmed2* and Fakharuddin2
...ions to generate 7 x 7 diallel crosses. In 2008 and 2009, all F1 hybrids along with their parent cultivars were planted in randomized complete block (RCB) design with four replicates at Tobacco Research Station Mardan (plain) and Tobacco Research Substation Mansehra (hilly). Data for all traits were analysed in pooled analysis and individual environments. Heterosis over better parent was determined for all traits across environments. Heterobeltiosis...
Muhammad Jurial Baloch1*, Ghulam Murtaza Channa2, Wajid Ali Jatoi2, Abdul Wahid Baloch1, Imdad Hussain Rind1, Muhammad Ahmed Arain1 and Ayaz Ali Keerio1
...yield traits from half diallel crosses was carried-out. Mean squares due to GCA and SCA were significant for days to 75% maturity, tillers plant-1, spike length, spike density, grains spike-1, grain yield plant-1, seed index and harvest index. Significance of GCA and SCA variances suggested that both additive and non-additive genes were controlling these characters. The magnitude of SCA variances were higher than GCA indicating...
Muhammad Ishaq and Raziuddin
...ined in an 8 × 8 diallel intra specific F1 crosses in rapeseed (Brassica napus L.) during 2011-2013 to scrutinized potential lines, hybrids, and nature of gene action involved in the inheritance of maturity and plant architecture traits. Analyzed data revealed highly significant differences among the genotypes for days to flowering, maturity, plant height, primary branches plant-1 and main raceme length. Analysis of combining ability revealed highly sign...
Muhammad Nawaz Kandhro, Qamaruddin Jogi, Mahmooda Buriro, Aijaz Ahmed Soomro, Ghulam Mustafa Laghari and Ali Nawaz Khaskheli
... The utilization of allelopathic properties of plants is thought to be an effective and environment-friendly approach for weed management. A pot experiment to evaluate the allelopathic potential of leaves of Eucalyptus camaldulensis (L.) against Convolvulus arvensis (L.) and Cyperus rotundus (L.) was undertaken during summer 2011. The study was conducted at Department of Agronomy, Sindh Agriculture University, Tandojam,...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...e mutation. Genotype and allele frequency analysis showed that SNP G62976A and SNP G50831C of the HTT gene in chicken had some effects on reproduction traits, and the genotype GG was the advantageous genotype. Additionally, four HTT haplotypes (H1: GG; H2: GC; H3: AG; H4: AC) and their frequency distributions were estimated using the phase program. Haplotypes combinations constructed on these two SNPs of HTT gene were associated with some reproduction traits. ...
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...ed. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method. This study was conducted to determine three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed. In TGC samples, C allele freq...
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
...sm was carried out using allele specific PCR based amplification strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequencies were not deviant f...
Wazha Mugabe1,2, Lawrence Akanyang1, Mackenzie Nsinamwa1, Batanani Moatswi1, Naledi Matthews1, Kealeboga Dipheko1, Imtiaz Ahmed Ujjan3 and Assar Ali Shah2*
... each study area, two parallel transects measuring 1000 m radiating from the water points (borehole) were established. Sampling plots of sizes 10m x 10m were systematically placed along each transect at intervals of 50m for the first 500m and the last 500m the spacing interval was increased by 100m. The botanical composition (P<0.05) of fodder trees along grazing gradients was composed of 20 and 12 species for fenced and unfenced areas, respectively. The gr...
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...eatest capability for parallel yet specific testing and can be used to detect individual or combinations of viruses with sensitivity comparable to ELISA. Methods based on PCR provide the greatest sensitivity among the listed techniques but are limited in parallel detection capability even in “multiplexed” applications. Better, easier and cheaper than polymerase chain reaction or antibody detection, rolling circle...
Jakub Jan Skorupski
...e gene genotypes has tri-allelic polymorphism g.1219C>G (PIC = 0.3039), and three di-allelic polymorphisms - g.616G>C, g.846A>G and g.931C>T (PIC>0.2400). The recognized SNPs enrich the database of single nucleotide polymorphisms for American mink.
...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...eported. Heterozygosity, allelic and genotypic frequencies of identified variants were also determined. Chi2 test was performed to evaluate the Hardy-Weinberg Equilibrium of each polymorphic site. Two loci were found to be in HWE. These identified alleles will be useful for animal selection at molecular level i.e. an ideal tool for marker-assisted selection of animals for future breeding programs.
...
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...ction in body weights parallel to increase in dose concentration, as compared to control and vehicle control. Morphometric studies showed significant differences in nervous, integumentary and skeletal systems against control. Exencephaly, Omphalocoel, hygroma and limb abnormalities were recorded. It is shown that Cr VI causes teratogenic effects in mice embryos.
Süleyman Kök*
...lt;0.001). The effective allele number and allele density are other criteria to show the spatial heterogeneity in a population. The average effective allele number (ne) per loci for TGC samples from the smallholders in the villages, TGC ex-situ conservation herd and the total population sample were calculated as 1.7379±0.3559, 1.6026±0.4422 and 1.7103±...
Yun Wang1,*, Lei Guan1, Jiding Chen1, Yaping Kong1, Lang Si2 and Asif Shah3
...R) tracks are running parallel to each other with an in between distance of 0 to 2.5 km from Kunlunshankou to Wudaoliang sections which is about 107 km. An expressway will be built along these two lines in the near future to improve further accessibility between Qinghai and Tibet. Understanding the overlapping impact of QTH and QTR on ungulates will facilitates locating the route selection parameters for the proposed upcoming expressway. Using the procedures o...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
... and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency of HLA-DRB1*11 and HLA-DRB1*12 allele in aeroallergen sensitized individuals. Blood samples of aeroallergen sensitized individuals were collected at the Allergy Centre, NIH, Islamabad. Then samples were analysed at the Department of Microbiolo...
Muhammad Imran Shabbir
...of paternal and maternal alleles for a particular cross. Described here is a line-dot method using ternary genotype codes to determine genotype probabilities in a very quick and simple way. Also ternary genotype codes along with gender symbols and affection status are used to modify the Punnett squares. On applying these modified Punnett squares along polygonal shapes covers all possible parental combinations and their genotypic and phenotypic outcomes in offs...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...rated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.201 to 0.630, respectively. The polymorphic information content value ranged from 0.181 to 0.573. Null allele frequency varied from -0.0548 to 0.0563. The sets of loci proved to correctly discriminate populat...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...02). The prevalence of C allele was 13.5% in disease, 5.83% in controls and found significant with p-value 0.007. These findings suggest that c.G745C in COMT gene has a significant association and a possible role in the OCD development in Pakistani patients.
...
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...her frequency of the Ser allele compared to the controls (22.9% vs. 18.3%; OR: 1.32, CI: 0.95–1.83, p=0.013). The minor allele (Ser) showed a significant association with weight, BMI, fasting plasma glucose, total cholesterol level and LDLC had no effect on age, height, triglycerides, HDLC and leptin. In conclusion, Gly482Ser variant appears to be significantly associated with diabetes in Pakistani population. T...
Mian Ahmad Raza*, Ghulam Hassan and Naqib Ullah Khan
...important traits using diallel mating design in wheat at Peshawar Pakistan. Eight wheat genotypes viz. Janbaz (JNB), Fakhr-i-Sarhad (FS), Pirsabak 2005 (PS), AUP-5008 (AUP), Saleem 2000 (S2K), Tatara (TTR), Barsat (BST) and Siren (SRN) were crossed in all possible combinations during 2011-12 cropping season. The resultant F1 hybrids were planted following a randomized complete block design using three replications in the subsequent wheat growing season of 2012...
Shengyan Su, Zaijie Dong*, Wenbin Zhu, Lanmei Wang and Jianjun Fu
...ch-up like growth from diallel cross-breeding due to its similarity with high growth speed in the later period for common carp Cyprinus carpio L cross-breeding. A compensatory index matrix for 3×3 full diallel cross combinations used for selection was built to investigate compensatory like growth of each combination. Yes/No(Y/N) effect was determined for specific combination dyads, which there were significant d...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...a PCR-RFLP. Genotype and allele frequencies were calculated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to determine statistical significance. The frequency of 54Thr allele of FABP2 significantly associated with MetS risk in Pakistani population. Our results showed that frequency of the FABP2 54Thr allele was signi...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...equency of genotypes and alleles was respectively: AA=0.0469, AG=0.3154, GG=0.6377, A = 0.2046 and G = 0.7954. AA homozygotes had the highest age at first insemination and the lowest insemination index. Cows with this genotype after calving and calves (both gender) from AA homozygotes had the biggest weight after calving. In contrast GG homozygotes had the longest intercalving period. The results prove the existence of dependencies between genu <...
Meghan Griffith
...are in important ways parallel to a more recent (“Modern”) argument for the incompatibility of causal determinism and freedom to do otherwise” (1). Comparisons between foreknowledge and determinism appear throughout the book, typically when Fischer is defending incompatibilist arguments regarding foreknowledge and freedom to do otherwise (i.e., alternatives). But in an intriguing discussion in the introductory chapter, Fischer draws a compari...
Barkat Ali*, Muhammad Shahid Iqbal, Muhammad Kausar Nawaz Shah, Ghulam Shabbir** and Nasir Mahmood Cheema***
...nbsp;-A 6 x 6 complete diallel mating design was used to study the inheritance of different plant traits in upland cotton (Gossypium hirsutum L.). Six genotypes viz., BJA-592, ACALA-SJ-4, 4-F, CP-15/2, CIM-497 and PB-899 were included in diallel crosses. Results revealed that over-dominance type of gene action was controlling the inheritance of monopodial and sympodial branches plant height number of bolls and boll weight. T...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...ang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymorphic loci and 4 moderately polymorphic loci. The average expected heterozygosity (He) and observed (Ho) heterozygosity in four populations ranged from 0.6421-0.6980 and from 0.4841-0.6999. Significant deviations from Hardy–Weinberg were found in the fou...
Lin Wang1,2, Ye Gong3, Kelin Chen1, Haitao Wang3 and Xianguo Lyu1,*
...nd polymorphic with 2-10 alleles. The observed heterozygosities ranged from 0.481 to 0.827 and the polymorphic information content ranged from 0.373 to 0.775. Significant linkage disequilibrium was found only between two markers. These microsatellites could be used to enhance our understanding of genetic information and breeding biology of Relict Gull.
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