Muhammad Shah Zaman1, Ghulam Muhammad Ali2, Aish Muhammad1, Khalid Farooq2, Iqbal Hussain1*
...miarid regions. In vitro screening of eight potato varieties (Asterix, Cardinal, Challenger, Desiree, Hermis, Kroda, Sh-5 and Sante) was investigated for salt tolerance at 0.0, 10, 20, 40, 60, 80, 100 mM NaCl supplemented with MS medium. Kroda emerged as the most salt tolerant variety with the highest plant height (6.5 cm), number of nodes (8.8), fresh shoot weight plant-1 (0.166 g), number of roots (4.6) and root length (2.5 cm) at 60 mM NaCl followed by Sh-...
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
...y, including efficiently screening the antivirals against CSFV and viral receptors, conveniently tracking the viral proteins in live cells as well as improving diagnostic methods and vaccines for classical swine fever....
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...an ELISA-like microplate-screening assay (ELASA). Top three candidate aptamers were then paired in a matrix of all possible capture and reporter aptamer combinations and tested in a fluorescent peroxidase-linked Amplex® UltraRed (AUR; a resazurin-like substrate) version of a rapid (< 1 h) aptamer magnetic bead sandwich assay. The optimal sandwich aptamer combination utilized the same aptamer sequence (designated Rt-18R) for both capture and reporter fun...
Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren
...ody levels even when the screening extends over a couple of weeks. For the long term consistency, a significantly higher mean ADV antibody level was recorded in February compared to in October. Therefore, if the VP2 ELISA should be used as a tool for selection of breeding females in a control program for AD, the optimal sampling time at which the ADV antibody levels best can predict the breeding performance of the female needs to be further evaluated.
Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1
Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1
...A (c-ELISA) were used as screening tests, and overall seroprevalence of brucellosis was found to be as 21%, 21% and 13% by RBPT, SAT and c-ELISA tests, respectively. Seroprevalence was higher in females (26%) than in males (16%) by RBPT and SAT tests, and also in camels less than 9 years of age (13.33%). Milk samples were collected to detect the antibodies against the disease, however, none of the sample was found to be positive for brucellosis. It was conclud...
John G. Bruno
... aptamers from the ELASA screening were then attached to streptavidin-coated magnetic beads (SAv-MBs) and used to capture their cognate and related E. coli serovars. Capture was assessed by fluorescence microplate assessment following acridine orange staining. While there was significant cross-reactivity between the various captured serotypes, each of the anti-LPS aptamer candidates showed a preference for its cognate E. coli serotype. Results suggest that the...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
...t with the aim to assist screening of various fungal species for antimicrobial natural products. During this study five different fungi, including Alternaria alternata, Acremonium sp., Alternaria brassicicola, Pythium sp. and Aspergillus flavus were screened against Wilt causing pathogens using the dual culture assay. Among them, Aspergillus flavus was found active fungus against the selected vascular Wilt causing fungus and bacteria, i.e. Fusarium oxysporum (...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
...digenous cattle. Routine screening and strict biosecurity measures are recommended for prevention of infection in animals. Humans must consume milk after pasteurization.
...
John G. Bruno
...er sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies when captured by their Fc tails on the surface of Protein A-conjugated magnetic beads. While no clear similarities in the partial or complete primary DNA sequences or secondary stem-loop structures of these aptamers were observed, 3-dimensional computer models of these top ten anti-idiotypic aptamer candidates showed some topological similarities which suggest common bi...
Syed Ishfaq Ali Shah1,*, Tassawar Hussain Malik2, Imran Rafi Khan1 and Zahid Hussain3
...nt to the disease. After screening, the most resistant accessions of USDA were USG-14-2464 and USG-14-2478. However, USG-14-2481 and USG-14-2484 were recorded as partially resistant to CLCuV disease. Among the local germplasms, all accessions screened were found extremely susceptible. Disease index increased with day after planting (DAP) and high infestation level of whiteflies as it was positive and non-significantly correlated with DAP and whitefly on both U...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...st report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorphisms (SNPs) were reported. Heterozygosity, allelic and genotypic frequencies of identified variants were also determined. Chi2 test was performed to evaluate the Hardy-Weinberg Equilibrium of each polymorphic site. Two loci were found to be i...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
Bushra Mazhar1, Nusrat Jahan1, Nazish Mazhar Ali1, Saiqa andleeb2, Shaukat Ali2*
...rice bran oil. After the screening of different bacterial isolates, a novel strain Enterobacter hormaechei showed significant production of vanillin when inoculated in waste residues of rice bran based media. E. hormaechei produced 5.2 g/l of vanillin in culture media containing ferulic acid as a substrate. Optimum pH for E. hormaechei was 7 and the temperature was 30°C. Using optimum growth conditions and suitable fermentation medium, E. hormaechei can be...
Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz
Aqsa Waqar1, Sahir Hameed Khattak2, Sania Begum2*, Tayyaba Rehman1, Rabia1, Armghan Shehzad2, Wajya Ajmal2, Syeda Shahdana Zia2, Iqra Siddiqi2 and Ghulam Muhammad Ali2*
...rs is efficient tool for screening diversity of rust genes in wheat germplasm and can facilitate the integration of multiple genes into wheat by pyramiding and transformation. This review discusses information regarding rust disease and resistance in wheat to tackle the disease through resistance breeding. ...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
... blood clot lysis led to screening of mutants UV 2, UV 6, UV 7, UV 9, UV 11, UV 12, UV 15 and EB 3, EB 4, EB 11, EB 13 with hyperactivity as compared to wild bacterial strain. Out of the total 15 isolates of EtBr-treated strains, EB4 gave the maximum enzyme activity (7.3 ± 0.005 U ml-1) with a zone diameter of 6 mm. While UV6 was found to be the best UV-induced mutant with an enzyme activity of 20.333 ± 0.006A U ml-1
Ayesha Saeed*, Mobina Naqvi and Ahsan Javed
...t in neonates. Antenatal screening of food insecurity and timely provision food supplements through social welfare can help reduce neonatal morbidity and mortality. ...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of clinical mastitis was 10.2% (106/1036 animals) as a whole. However, the prevalence of clinical mastitis in district Lahore was 11.8% (71/598 animals) whereas it was 8% (35/438 animals) in district Bhimber. The overall prevalence of subclinical mastitis was 38.8%...
Fouzia Tabssum1* and Syed Shahid Ali2
...getables wastes. Initial screening of pectinase producing bacteria was carried out on pectin containing agar plates of pH 7 incubated at 37ºC. Among isolated thirty-seven strains, a total of thirteen strains showed satisfactory pectinase productivity. Pectinase production of the thirteen strains was then determined qualitatively as well as quantitatively. Zones of hydrolysis produced by the bacterial strains were ranged from 0.9-2.6 mm2 in diam...
Fouzia Tabssum1* and Syed Shahid Ali2
...getables wastes. Initial screening of pectinase producing bacteria was carried out on pectin containing agar plates of pH 7 incubated at 37ºC. Among isolated thirty-seven strains, a total of thirteen strains showed satisfactory pectinase productivity. Pectinase production of the thirteen strains was then determined qualitatively as well as quantitatively. Zones of hydrolysis produced by the bacterial strains were ranged from 0.9-2.6 mm2 in diam...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...ervals. The confirmatory screening for HBV and HCV was performed through Chemiluminescent Immunoassay (CLIA). Of the total 1,440 patients studied, 930 (64.6%) were males and 510 (35.4%) were females. The patient age ranged from 1 to 30 years were with mean age of 7.9±4.5 years. Among 1,440 patients, 44 patients were positive for HBV (3.05%) while 295 were positive for HCV (20.4%). This study showed that β-thalassaemia patients are at a higher risk ...
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...The ICT resulted in poor screening for HCV infections. The main route of HCV transmission in males is the sharing razors (33.33%), while in females, is the reuse of contaminated syringes (42%) at maternity homes respectively. These alarming results call for initiation of an emergency based awareness campaign about HCV among the public of district Shangla to prevent the impending epidemics of HCV. Additionally, for proper and actual results, the ELISA and RT-PC...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...ochin. The antimicrobial screening of Streptomyces against S. mutans showed that these strain are the prolific producers of active agents to inhibit the growth of caries causing S. mutans. The natural extracts of Streptomyces strains significantly inhibited and delayed the biofilm formation by S. mutans and a profound effect was observed in case of the extracts of Streptomyces strains HU-09 and HU-10, which delayed bio...
Shamsher Ali1*, Sarmad Khan1, Muhammad Jamal Khan2, Naveed ullah4, Muhammad Rashid3 and Wiqar Ahmad1
Musrrat Fatima, Muhammad Saad Khan, Hamid Rashid, Asim Mehmood, Sumaira Kanwal, Muhammad Asif Rasheed and Farrukh Jamil*
... structure-based virtual screening. After screening 100,000 compounds, 14 compounds are selected, which fulfilled different already established drug likeliness parameters such as rule of five and nontoxic nature. These 14 compounds are used for pharmacophore modeling, and Zinc natural compounds database (ZND) is screened against the developed pharmacophore. The compounds with the highest pharmacophore fit scores are screened...
Sadaf Noveen1*, Shaista Habib Ullah2, Babar Alam3
...on Disorders. Oral motor screening of 140 children showed that (23%) had OMM defects among these 33% had substitution errors, 60% distortion errors, and 3.03% omission errors whereas no addition errors were found. The correlation of 0.763 positive relationships between two variables was noticed. High percentage of distortion errors among children having OMM defects shows strong connection between the two otherwise substitution is most prevalent error.
<... 
Rizwana Qamar1, Maria Ghias1, Fida Hussain1, Sajida Habib1, Muhammad Khuram Razzaq2*, Muhammad Aslam1 and Imran Habib3
...earch Faisalabad for the screening of genotypes against drought. Ten hybrids along with their 13 parental inbred lines were grown in polythene bags following factorial structured completely randomized design by three replications against three levels of water stress. Data was recorded on root length (cm), shoot length (cm), plant fresh weight (g), plant dry weight (g), chlorophyll fluorescence, leaf temperature (°C) and relative water contents (%). Results...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...sent study describes the screening and production of extracellular lipase (EC 3.1.1.3) from a Rhizopus oligosporus mutant strain developed by chemical mutagenesis. The wild-type was simultaneously treated with different concentration of EMS and MMS. Among 13 mutant variants, EM-7 showing maximum lipase activity of 71±3.55 U/g, was selected. Resistance in the hyper producing strain was developed by exposing it with low levels of L-cysteine ...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...dy focused mainly on the screening of genetic marker V158M located in COMT gene for its relation to physical aggression in the four domestic bovine species of Pakistan, which are normally less aggressive. Selected animal species were cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V158M is present in exon-8 of COMT gene. Each specie was subjected to blood sampling (n=20 ea...
Muhammad Sarfraz*, Shahida Hasnain
...E. coli; preliminary screening for AmpC beta-lactamase production was done by cefoxitin disc followed by inhibitor based confirmatory method. Antibiogram was performed following CLSI guidelines 2013. Of 11,725 clinical specimens, 29 % were culture-positive. 80% K. pneumoniae and 70% E. coli showed resistance to cefotaxime or ceftazidime. Only63% K. pneumoniae and 58% E. coli and were resistant (<18 mm) to FOX. Phenotyp...
Muhammad Haroon Hullio1,4, Abdul Ghani Lanjar2, Abdul Rahman Dhuyo3, Imran Khattri4 and Agha Mushtaque Ahmed4*
...). In last, the varietal screening of 30 local rice varieties in term of pest population was observed. The data were collected for two rice growing seasons 2012 to 2013. In results, the maximum mean population of 15.25 WBPH/25 hills at 38 0C with 50% relative humidity was observed in district Badin in the first week of August, 2012. The correlation results regarding mean population of WBPH also indicated effect of temperature and relative humidity (r= 0.51, 0....
Ghulam Abbas1, Khalid Pervez Akhtar1, Muhammad Ahsan2, Muhammad Jawad Asghar1, Fiaz Ahmad1 and Muhammad Rizwan3*
Dilara A. Bukhari1,*, Naureen Fatima1 and Abdul Rehman2
...esent study was aimed at screening the local Bacilllus thuringiensis (B.t.) isolates for Cry11 and assess their potential use for mosquito control. A total of 15 B.t. isolates were collected, of which 75% were from dry, leaf litter, garden soil samples, 15% were from animal waste and 10 % were from moist soil of the crop area. A 650bp of cry11 gene was amplified by PCR and seven isolateswere found positive for cry11 gene. The 16S rD...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
...esents the diagnosis and screening of suspected rabies samples of cow and mule. Clear behavioral changes along with paralysis of tail and hind legs were noticed in the mice of both groups between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT). Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying the highly conse...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...quencing. For biological screening different methods including cross streak, agar plug and well diffusion method were performed. Seven Streptomyces strains including NWMD-6, NWMD-7, NWMD-10, NWMD-11, NWMD-12, NWMD-15 and NWMD-16 were found to have significant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse metabolic compounds...
Tahira Jamil*1, Mohammad Asghar1, Fatma Husain1, Haq Nawaz Bhatti2
Farah Mansha, Abdul Rehman*
Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3
Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1
Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
Shamsher Ali1*, Muhammad Jamal Khan2, Zahir Shah2, Naveedullah3 and Abdul Jalal1
Ajmal Khan Kassi1*, Humayun Javed1 and Tariq Mukhtar2
Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3*
...; standardized germplasm screening protocols as well as optimized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculatio...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...and fusidic acid. Proper screening of MBLs producing GNRs should be done from clinical specimens along with antimicrobial susceptibility testing for developing an effective treatment strategy and thus better control of infections caused by these bugs in future.
...
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
...bacterial and antifungal screening of aqueous methanolic extracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against...
Arjumand Nizami1, Jawad Ali1 and Muhammad Zulfiqar2*
Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1
...t (SFMT) was applied for screening subclinical mastitis followed by bacteriological techniques on positive milk samples for confirmatory isolation of Staph. aureus as a bovine mastitis causing agent. These Staph. aureus isolates were further tested for antibiotic susceptibility. Epidemiological data was analyzed by chi-square methods at 95% confidence interval. The overall prevalence of subclinical mastitis as a result of surf field mastitis test...
Bata Shalangwa Ishaku1, Batim Turdam1, Maimadu Abdullahi1, Waziri Ibrahim Anjili2 and Mayowa Olabode2
...control measures such as screening and treatment of infected animals are therefore recommended. ...
Muhammad Zain Saleem1,*, Raheela Akhtar1, Asim Aslam1, Muhammad Imran Rashid2, Zafar Iqbal Chaudhry1, Muhammad Adeel Manzoor1, Bilal Ahmed Shah3, Rais Ahmed4 and Muhammad Yasin5
...rams rely on serological screening of brucellosis rather than molecular assay which could confirm the particular species circulating in ruminants. In this study 960 and 471 serum samples of goats and sheep were collected, respectively. After screening with Rose Bengal test (RBT), all seropositive samples were subjected to real-time PCR assay. RBT confirmed the seroprevalences of 19.32% ± 0.289 and 12.29% ± 0.01...
Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3
...s Test was performed for screening of positive samples. Afterward, the bacterial profile was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within this, contribution of Gram-negative bacteria was 68% and that of Gram-positive bacteria was 32%. Among 30 positive samples, percentage prevalence of different bacterial species was: E.coli (37%), S. aureus (23%), Pseudomonas (20%), Streptococcus (10%), Proteus (7%...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
...n for the future genetic screening of the breast cancer patients of Balochistan.
...
Shamsudin Bojang1, Idris Abd Ghani2, Jugah Kadir1, Adamu Saidu Paiko1, Yasir Iftikhar3 and Muhammad Kamran3,4,*
...e discounted. Therefore, screening and restriction of movement of planting materials be observed critically.
...
Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain1
...nd secondary qualitative screening, One isolate (PAK-01) exhibited maximum proteolysis on 1 % skim milk while another isolate (PAK-03) demonstrated maximum lipolysis on Tributyrin Plate Assay. On the basis of morpho-physiology, biochemistry and 16s rRNA sequence, the PAK-01 was identified as Stenotrophomonas sp. MG662181 and PAK-03 was identified and tagged as Pseudomonas sp. MG687270. Based upon genetic signatures and physiological parameters, our strain pres...
Qudrat Ullah1,8,*, Huma Jamil1, Laeeq Akbar Lodhi1, Zafar Iqbal Qureshi1, Shakeeb Ullah1, Tariq Jamil2, Iahtasham Khan3, Shahbaz Bashir4, Qudratullah5, Inamullah Wazir6, M. Abdus Sallam1 and Muhammad Zubair7
...associated. Routine herd screening, consumtion of pasteurized milk and public awareness programmes would be helpful for control and prevention of zoonotic transmission.
...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...s a molecular marker for screening of mastitis resistant and susceptible Sahiwal cows.
...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...ta will not only help in screening of animals but will also be instrumental in future for isolation of MAP to make vaccine against paratuberculosis using local isolates.
... 
