El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...ishment of infections in vaccinated poultry. The Egyptian H5N1 viruses remain susceptible to oseltamivir, but genetic markers indicating resistance to amantadine have increased dramatically. In this mini-review, we discuss recent findings on the H5N1 viruses endemic in poultry and humans in Egypt, and suggest possible options to control the infection....
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...erentiating infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based vaccines, bivalent/multivalent vaccines, recombinant viral vectored vaccines, RNA interference as a therapy, suicidal DNAs, synthetic epit...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...was investigated in an unvaccinated mixed herd of sheep and goats. Goats in the herd showed characteristic signs of PPR including nasal and ocular discharges, high temperature, diarrhea and ulcerative lesions in the oral cavity. A total of eighteen goats from a herd of sixty, were affected and two goats succumbed within two weeks. Interestingly, the disease was exclusively observed in goats and all sheep kept in the same herd were serologically positive but di...
Ahmed Zein Mahmoud, Muaz Abdellatif and Ahmed Abdalla
...nd camels. Sera from non-vaccinated sheep (n=683), goats (n=624) and camels (n=155) of all ages and sexes were collected in a cross-sectional study in Hail, Bagaa, Shenan and Ghazalah. Saudi Arabia. The seroprevalece was determined by NP-epitopes based competitive ELISA. The overall prevalence was 59.9%, goats had a significantly higher sero-prevalence of 75.3% compared to 59.4% obtained from sheep, whereas camels were seronegative. The prevalence of PPR was i...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
...lular processes. Between vaccinated and unvaccinated groups, there were 13 and 17 up- and down-regulated DEGs (2-fold; FDR < 0.05; P < 0.001), respectively, in PBMC of PEDV. Most of significant DEGs were involved in multiple cellular processes of cell growth and immune system. Thus, results from our current study indicate that DEG analysis of RNA-seq data help us better understand the precise mechanisms of PEDV ...
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
...ded A, B, C, D, E and F; vaccinated with various combinations of IBD through different routes. Results showed that the birds of different groups received different maternal antibodies did not induce any antibodies as recorded in all groups received vaccines at days 1, 9 or 12 exhibited similar pattern antibody profiles, however, an increase in the mean titer of antibodies was recorded from 17 and onwards. Furthermore, on day 39 of age, the birds of all groups ...
Uzma Farid Durrani1,*,Sagar Mal Goyal2, Asim Khalid Mahmood1,Cathleen Hanlon3, Susan Moore3, Muhammad Waqas1 and Raheela Akhtar4
...helped by dams regularly vaccinated with high quality inactivated rabies vaccines. Rapid Fluorescence Focus Inhibition Technique (RFFIT) revealed mRVNA titer (>0.5 IU/mL) in 29 puppies while 48 puppies showed mRVNA titer less than minimum protective level (0.2-0.4 IU/mL). RFFIT did not reveal mRVNA in 23 puppies that were declared lacking maternal rabies immunity. One basis of findings of this study it was concluded that puppies population lacking maternal ...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...icated that all chickens vaccinated with pCA-Egy-H9 were fully protected without clinical signs and virus shedding. Our results revealed that the pCA-Egy-H9 plasmid DNA vaccine could induce complete protection in chickens against H9N2 virus challenge and may propose an alternative strategy to control virus infections in the poultry industry....
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...cial broiler chicks were vaccinated with LaSota. A group of chicks (n=40) were immunized using baculovirus (H5 and ND) inactivated vaccine via subcutaneous route. Birds (n=20) were then either challenged with 106 EID50 using Egyptian avian influenza H5N1 and Newcastle disease genotype VII. Comparative blood profiling in vaccinated and challenged, non-vaccinated and non-challenged and non-<...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
...eneous appearance. Birds vaccinated with vaccine A showed mean ELISA antibody S/P ratios from 0.099 to 0.995 between weeks 2 and 6 post-vaccination against IBV antigen, while for vaccine B group mean S/P ratio dropped to 0.550 at week 6. Virus neutralization test showed that birds vaccinated with vaccine A showed neutralizing antibodies against IBV Massachusetts, 4/91, D274 and Qx types, while titers for vaccine B were not d...
Nukhbat-Ullah Awan1*, Muhammad Sohail2, Zeeshan Sarwar3, Muhammad Mustahsen Bashir4, Muhammad Abbas Khokhar5 and Asad Aizaz Chatha6
...) of patients were never vaccinated. Incubation period noted was 7±1 days. Tracheostomy was performed in 16±3 hours of admission and tracheostomy decannulation was performed in 23±3 days. Patients were discharged in 27±3 days.Weconcludethat early control of airway with tracheostomy in grade II tetanus patients helped in better patient management with early recovery and decreased hospital stay with minimal morbidity. ...
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...nger than 3 months and unvaccinated. Distance genealogy using CCoV sequences from GenBank for M gene showed that eight strains were CCoV-II twenty-six were CCoV-I. These findings show some genetic features of CCoV in Brazil and may require future studies to elucidate full genome sequences of these isolates to better assess the disease transmission dynamics and future control strategies. ...
Shahid Ali1* and Salamat Ali2
...farmers also need to buy vaccinated day old chicks from standard hatcheries for fostering boiler productivity and net revenue. Moreover, use of high quality feed for highest possible output is a good option for open shed farmers.
...
...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...IV H7 and AIV H5) in non-vaccinated wild and domesticated bird species across Pakistan. During January-December 2013, in total 700 samples were collected from different species of non-vaccinated birds inhabiting diverse ecological zones of Pakistan. Altogether, 507 tissue and swab samples were screened for the presence of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR). Same samples were further process...
Fatma Abdallah1*, Ola Hassnain2, Elsayed Attar3, Haytham Ali3,5, Mohamed Megahed1 and Venugopal Nair4
...as conducted. Twenty one vaccinated chicken flocks suffering from different form of lymphomas, representing different governorates in the Egyptian Delta, were screened for MDV infection. Pathological findings in the visceral tumours and presence of viral DNAs with variable mitotic figures were observed in the examined visceral organs. Eight samples were screened positive for the major MDV-encoded oncogene Meq which was subjected for alignment and phylogenetic ...
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...ne titer in animal group vaccinated with toxoid vaccine prepared from pathogenic isolates and provide best protection against challenge infection.
...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2
...ccount it was found that vaccinated animals showed very low prevalence, i.e. 4.39% as compared to non-vaccinated animals having 66.53%. Another risk factor age suggested that younger animals were more affected (60.17%) as compared to older ones (29.41%). It was also observed that animals in closed housing system were more affected as compared to open housed animals. When comparative efficacy of diagnostic tests was do...
Omer Baris Ince*
...t were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the disease-related status of the selected region, to investigate the active virus circulation and determine the antibodies formed against the nonstructural proteins (NSP) of Foot and Mouth Dise...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
...ks have occurred even in vaccinated flocks. Commercial Newcastle disease virus vaccines can provide various levels of protection against challenge with different NDV genotypes, raising the importance of the relationship between vaccines and field strains. The aim of the current work is to evaluate the effect of moisture percentage and pH on the persistence of velogenic NDV genotype VII in the poultry manure which considers a major threat to the Egyptian poultr...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...e groups and were orally vaccinated, respectively SS-14 (Group T1) and SS-28 (Group T2) DNA vaccines fused tPA signal peptide and CpG adjuvant and delivered by attenuated Salmonella choleraesuis at weeks 0, 3 and 6 of the study, and rats in control group (Group C) was orally given empty vector vaccine. Blood samples were collected before primary immunization and at weeks 3, 5 and 7 after primary immunization and body w...
Rana Waqas Arshad1, Asim Aslam1, Muhammad Saeed Imran1, Kamran Ashraf2 and Raheela Akhtar1,*
...on was observed in birds vaccinated with immune complex or intermediate plus vaccines. Consistently, histopathological lesions of IBD were less evident in live vector vaccine group in comparison to other groups. In addition live vector vaccine improved the feed conversion ratio (FCR) by keeping the bird healthy and by decreasing the immunosuppression. These results indicated that live vector vaccine has overall positive impact in terms of immunity, histopathol...
Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan
...dustry in Egypt, even in vaccinated flocks. A total of 8121 different samples (organs and swabs) that represented 588 NDV suspected flocks were collected from poultry farms showing respiratory manifestation and/or drop in egg production during 2015-2018. Out of 588 NDV outbreaks 95 flocks were confirmed positive NDV by rRT-PCR using genotyping primers, with a prevalence rate of 16.2%. NDV outbreaks were recorded in 16 governorates, from total of18 investigated...
Mumtaz Ali Khan1,*, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Ikramul Haq4, Kashif Prince1, Asad Ullah5, Muhammad Shoaib2, Shahid Zaman6, Amjad Islam Aqib7, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4,Imad Khan5, Naimat Ullah4 and Muhammad Shahid8
...mals of both groups were vaccinated with enterotoxaemia vaccine. Group C was kept as negative control in both rabbits and goats. The immune titer against C. perfringens type D was evaluated on 0, 7, 14, 28th and 60th day of vaccination with indirect haemagglutination test (IHA). The results showed significantly higher (P< 0.05) immune titer in vitamin E supplemented animals groups against C. perfringens type D epsilon tox...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...cally characterized from vaccinated birds collected between 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99.8% with similar viruses in this clade. Analysis of the hemagglutinin (HA) gene sequences of the two AIV was performed and phylogenetic analysis reveals genetically closely-related to H9N2 viruses classified into Mideast group-B and sub-lineage B2. The two strains classified ...
Biomarkers for Pathogenic Clostridium perfringens in Small Ruminants of Khyber Pakhtunkhwa, Pakistan
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Naimat Ullah Khan3, Muhammad Asfandyar Khan2, Kashif Prince1,*, Mahboob Ali1, Ghazunfar Rashid1 and Azmat Ulah Khan2
...kids population from non-vaccinated dams of selected districts of Khyber Pakhtunkhwa province, Pakistan. Samples were obtained from non-vaccinated lambs and kids with the signs of enterotoxaemia. The isolates were initially identified by colony characteristics, Gram staining, biochemical tests and CFU/g. Clostridium perfringens was isolated from 43.45% of lambs and 50.59% of the kids. Obtained isolates were further an...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...respectively. Birds were vaccinated against Newcastle disease virus and infectious bursal disease. Relative lymphoid organ weights were recorded on day 42. Hemagglutination inhibition (HI) assay was performed against Newcastle disease virus on a weekly basis, whilst a hemagglutination (HA) assay was performed to determine immunity against total sheep red blood cell (SRBC) , mercaptoethanol-2 resistant (IgG) and mercaptoethanol-2 sensitive (IgM) antibodies on w...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1, Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
... 57.01 % respectively in vaccinated group as compared to positive control at 3rd, 4th and 5th week of immunization. Impact of the HcCP on haemoglobin level was also evaluated. After second week of infection significant difference (P<0.05) was found between the negative and positive control. However no significant difference (P>0.05) were found between vaccinated and control groups....
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...commercial chickens were vaccinated with La Sota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (NDV+IBV-challe...
Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5*
... Moroccan children fully vaccinated with Rotarix™. After RNA virus extraction, the rotavirus VP7 and VP4 genes were amplified. The DNA was purified, sequenced and genotypes were determined using the RotaC online classification tool. A phylogenetic tree was constructed using the Maximum Likelihood method applying the Tamura 3-parameter model in MEGA 6.06 package and statistically supported by bootstrapping with 1000 replicates. The amino acid sequences of...
Rabia Riaz1, Khushi Muhammad1*, Masood Rabbani1, Muhammad Amjad Iqbal2, Aamir Riaz Khan2, Sadia Sarfaraz1, Mudassar Naseer3 and Khalid Majeed1
... A, B, C and D (n=8) was vaccinated (subcutaneous: 0.3ml) with G-MG, O-MG, G-MG+NDV (New castle Disease Virus) and O-MG vaccines, respectively. Serum samples were collected on 21, 28 and 35 days post vaccination for anti-MG-ELISA antibody titer. Cumulative mean anti-MG-ELISA antibody titer of birds to G-MG, O-MG, G-MG+NDV, O-MG+NDV and control was 80.30, 137.49, 91.59, 169.76 and 0.15 units respectively on 35 days post-vaccination. It is concluded that NDV has...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...ruses were isolated from vaccinated commercial broiler flocks showing respiratory manifestation in Menofia governorate, Egypt during 2019. Those viruses showing deaths and haemorages of inoculated SPF ECE eggs, harvested allantoic fluids showed haemagglutination activity by using 1% RBCs and also haemagglutination inhibition in case of using NDV reference antiserum, As well as realtime polymerase chain reaction by using standardized NDV specific primers and fi...
Hiam M. Fakhry and Assem A.A.
...ps ; the first group was vaccinated intramuscularly (I/M) with trivalent foot and mouth disease zeolite (1 μg/dose) vaccine, The second group was vaccinated with foot and mouth disease oil vaccine and Third group was vaccinated with foot and mouth disease ( oil + zeolite ) vaccine. The cellular immune responses were monitored in different tested groups that revealed the immune responses...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being significantly lower prior to challenge, it provided good protection (73.3%) against the lethal A/chicken/Egypt/SHAH-1403/2011 (H5N1) AIV challenge, with no evidence of virus shedding. Keeping in mind that each bird was potentially challenged with 105 EID50, which may not be available to all birds under field condition. The foremost vaccine regimen can provide ...
Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5
1Mona A. El-Manzalawy, 2Abeer A. Boseila , 3Hanan M. El Zahed
...ization test (SNT). This vaccinated group of sheep was the highest while the group of sheep vaccinated with RVF vaccine adjuvanted with Alum gel only show the least antibody titer. The group of sheep, which was vaccinated with RVF, inactivated PMO without gel vaccine show intermediate level of antibodies. Moreover, the values of ED50 of the developed vaccines as well as the Alum vaccine al...
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
...y based on evaluation of vaccinated animals by detection of antibodies
against serotypes of FMDV (A),(O) and (SAT-2) by (SNT) as well as by analysis of negative
Non Structural Protein sera by solid phase competitive ELISA for differentiation between natural
infected and vaccinated animals by detection of Non-Structral protein of FMDV by priocheck
test. Five hundred sera were co...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...ating antibody either in vaccinated or latent infected horses....
Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1
...roups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 days all groups were challenged with a dose of 105.5 EID50/bird of Variant 2 genotype (1494) IBV through oculo-nasal rout. Blood samples were collected random...
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar
...ld Hubbard chickens were vaccinated with two different commercial live attenuated Chicken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux strain vaccine through drinking water (DW). Then the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV v...
Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed
...m apparently healthy non vaccinated cattle against LSDV and (100) serum samples were drawn from cattle 4 weeks post vaccination with local attenuated sheep pox virus vaccine located in Sharkia and Fayoum governorates. Methods: Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected cattle. The virus was isolated on MDBK cell line and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody te...
Lamya A. F. Ateya1 , Said. A .Ahmed2, Khamees K.S. Ashraf3, Heba A. Abdel-Hady4
...fication of infected and vaccinated animals. Our study was designed for assessment of vaccination by local and imported FMD vaccines in cattle sera in Kalubeya Governorate. Methods: 220 serum samples were tested for FMD NSP by 3ABC Trapping ELISA.20 serum samples were used as control (no vaccination),100 serum samples collected from animals received local vaccine and 100 serum samples from animals received imported vaccine. Results: 34/220(15.5%) were +ve and ...
Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.
...mean of 2 logs 10 in the vaccinated group
compared with control group.
Conclusion: the developed evaluation protocol could depend mainly on q PCR for identity and
titration of H5 insert gene in addition to vaccine safety and efficacy.
...
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
...enge experiment for H120 vaccinated broiler chickens were also performed. Results: Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide similarities and 96.8% and 91.4% amino acid similarities with the commonly used IBV vaccine Massachusetts strains H120 and M41 respectively. Egypt/Qal/014p had a unique amino a...
Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2
...) have been occurring in vaccinated chickens with great economic losses. Objective: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of NDV was amplified using Reverse Transcription-Polymerase Chain ...
Naglaa F.S. Awad1, Gamelat K.F. Kotb2
...e was collected from non-vaccinated young rabbit (45-day old) and designed here as SHAH
2015, whereas the second sample was collected from non-vaccinated adult dam (nine-month old) and
designed as SHMK2016. Hemagglutination analysis using human red blood cells type O revealed
that both isolates were non-hemagglutinating. Sequence analysis revealed that both isolates shared
amin...
Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden
...v>shedding. Challenge of vaccinated birds with AI vaccines under strictly controlled condition with
virulent HPAI virus may also be used to predict flock response to AI infection. The reduction in
challenge virus replication can be quantified using classical virus isolation (VI) and titration methods
in (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration...
Abdou Nagy1, Fatma Abdallah1, Kareem Sweed2, Ahmed Salama3, Mohamed Omar4
...evalence of aMPV for non-vaccinated chicken and duck
flocks from six provinces of Egypt (Ash Sharqiyah, Ismailia, Qalyubia, Menufia, Sohaq and North-
Sinai) and determine its economic effect.
Methods: Serum samples were collected from non-aMPV vaccinated apparently healthy 40 chicken
(23 broilers and 17 layers) and 8 duck (6 Pekin and 2 Muscovy) flocks and then, tested with
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
.... Groups 1, 2 and 3 were vaccinated with the prepared vaccine; group 4, 5 and 6 were
vaccinated with one of the imported reassortant H5N1 containing 300 HAU at 1, 5 and 10 days of age;
respectively. Groups 7 and 8 were positive and negative groups for the challenge trial. Blood samples
were collected weekly for 4 weeks of age and tested by HI test. Post challenge, tracheal and cloacal swab...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported reassortant vaccine with 500HAU antigen content of H5N1 at 1, 5 and 10
days of age; respectively. A group 7 was positive challenged control and group 8 negative challenged
groups. All chicken groups were maintained at isolators alon...
Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2
...ases of LSD in sheep pox vaccinated cattle in Egypt.
Objective: In this study LSDV was isolated from sheep pox vaccinated cattle showing LSD signs to
confirm LSD viral infection even in sheep pox vaccinated cattle.
Methods: 130 Nodular samples were collected from 150 vaccinated cattle showing signs of LSD,
...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...egy for EHV-1 viruses in vaccinated
and non-vaccinated animals needs to be addressed.
...
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...nd group-4 served as non-vaccinated control. The humoral immune response evaluated
serologically by using hemagglutination inhibition test (HI), serum neutralization test (SNT), and
indirect ELISA.
Results: There was detectable TRT antibody titers by SNT in the first week post vaccination as 4 log2
in group-1 and 2 log2 in group-3. Both groups showed peak SNT titer (256 log2) of TRT virus by the
third...
Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2
...zed in flocks which were vaccinated with classical IBDV
vaccines and demonstrated IBD characteristic lesions.
Objective: In present study the aim was to molecular characterize IBDV field strains detected in flocks
located in 4 governorates. Also, a phylogenetic analysis based on the sequence of the hyper variable
region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected fro...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2
Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein
...ibody response of calves vaccinated with conventional inactivated trivalent FMD vaccine. Expression of Th1 and Th2 was determined using quantitative ELISA. Results reveled peaks of IL-4 at 7th day, IFN-γ at 14th day and IL-2 at 21st day post vaccination. Lymphocytic proliferation assays demonstrated the continuous response of lymphocytes started at the 3rd day till reaches peak at 28th day post vaccination then start to decline. Whereas the peak titers f...
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...incomplete protection in vaccinated cattle, since that it was a
must to produce a homologues vaccine for LSDV.
Methods: A local isolate of LSDV strain was propagated on MDBK cell line and CPE was appeared
after 72 hours as cell rounding, cell aggregation and cell degeneration with titer of 105.5TCID50/ml,
DNA extraction and PCR for the candidate strain was performed using universal and specific primers
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...v>
were previously vaccinated using the Egyptian sheep pox (SPPV) vaccine, apparently healthy cattle in
contact with the diseased cattle, and one healthy animal located in a previously infected zone. LSDVspecific
PCR was used to detect LSDV in sampled ticks. PCR-positive tick samples were subject to a
modified RT-PCR assay to detect the polyadenylated LSDV DNA polymerase mRNA. Metagenomics
analysis using Mi...
Soad, E. Elsayed 1, Mohammed A. Shalaby2, Ausama A. Yousef2, Abu bakr A. Agor1,
Sherouk E. Aly1
...ed 90% of the Guinea pig vaccinated by nano emulsion.
Conclusion: Our findings suggest that we aimed to enhance immunity through nano emulsion of
Montanide ISA 206 is well achieved.
...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
...ity. Animals
vaccinated using the enhanced formula were completely protected against RVF challenge virus and
produced higher RVFV neutralizing antibody titers.
Conclusion: The vaccine developed based on small particle vaccine formulation is an economic
solution that can be added inline to existing production platforms to enhance immunogenicity.
...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
... contact
non vaccinated chickens using qRT-PCR test.The genotype VII (GVII) vaccine group
induced higher HI titer by using GVII monospecific antigen and decreased virus shedding
than other group. The vaccinated group with genotype VII significantly reduced the virus
shedding at 3, 5, 7 and 10 days post challenge unlike the genotype II vaccinated<...
Arsalan Khan1*, Navid Anjum2, Muhammad Shuaib Khan3, Shakeeb Ullah3,
Ali Zaman3, Kamran Safdar3, Khalid Muhammad3, Ambrina Tariq4,
Muhammad Inam Malik3, Muhammad Kamal Shah3, Atiq ur Rehman3
and Hamza Maris3
...r of ticks infesting the vaccinated and control group over 01-month period, post vaccination. The results revealed that physically the number of ticks were significantly reduced (P<0.05%) using crude larval extract vaccine (78.4%) than standard acaricidal drugs, ivermectin (63.24%) being conventionally used.
...
Daniel Stewart Robertson
...vidual has ideally to be vaccinated with attenuated versions of the virus particles produced by his or her own cells. Observations supporting the proposals are presented....
Lungile Gumede1,2, Thobela L. Tyasi1, Teedzai Chitura1*, Khanyisile R. Mbatha3
... experimental goats were vaccinated with 2 millilitres of blanthrax vaccine per goat via the subcutaneous route. Three experimental diets were formulated by replacing a conventional supplement of Lucerne with M. oleifera. The inclusion levels varied from 0% to 50%. Data on the growth and haematological parameters of the animals used in the study were analysed using a general linear model (GLM) procedure in a completely randomized design. The results indicated ...
Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1*
...m samples from eight non-vaccinated dairy herds. The collected serum samples (n=56) were examined for detection of leptospiral antibodies using the Microscopic Agglutination Test (MAT), the gold standard serological test. The Chi-square and odds ratio analyses were employed to identify the important risk factors for leptospirosis in dairy farms. The study revealed that the seroprevalence of bovine leptospirosis at an individual animal and farm level was 39.8% ...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...e collected from two IBD vaccinated native broiler chicken flocks showing clinical signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly characterized using reverse transcription polymerase chain reaction (RT-PCR) technique and gene sequencing of partial portion of VP2 gene. The two isolates (Genbank accession numbers MW925051 and MW92505...
Safaa M. Barghash1*, Amani A. Hafez2
...duces poor protection in vaccinated rats against P. multocida infection.
Keywords | Trypanosoma evansi, Pasteurella multocida, Vaccine, Rats, Egypt
... 
Tri Untari1*, Sitarina Widyarini2, Michael Haryadi Wibowo1, Marla Anggita1
...imens. Group I - IV were vaccinated NDV while group V-VII were unvaccinated. Each group was treated with either red ginger powder (group I and V), ethanolic extract (group II and VI), essential oil (group III and VII). Group IV served as the untreated control. The analyses were done by measuring the NDV antibody titers using hemagglutination inhibition test and histological studies to observe the toxic effect of red ginger t...
Mohamed A. Hussein1, Bahgat A. Abd El-Rehman2, Kareem A. Eldin2, Mohamed Fouad Mansour1*
...and 3th months in groups vaccinated with Ch-NPsV, AlP-NPsV and AlHV respectively. Conversely, neutrophil count decreased in vaccinated groups. No change was detected between groups in serum kidney function enzymes. Cytokine profile including IL-2 was markedly increased from 1st day after inoculation and elevated moderately till arrived to the peak at the 5th, 7th and 10th days post-vaccination in groups
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...oups (n=4), control, non-vaccinated and vaccinated in each season. After acclimatization and synchronization procedure, the vaccinated group was administered with 2 mL of alum-precipitated pasteurellosis vaccine while non-vaccinated and control groups were administered with 2 mL of phosphate-buffered saline (PBS) via the intramuscular route. At week 2, b...
Effects of Antibiotics on Growth Performance, Immune Response, and Intestinal Microflora of Broilers
Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5
...eriod. The chickens were vaccinated for NDV and AIV. None of the antibiotics adversely affected the development of NDV or AIV hemagglutination inhibition geometric mean titers. Flavomycin and zinc bacitracin did not adversely affect the mean splenic (1.27+0.20 g and 1.21+0.15 g, respectively), thymic (3.42+0.26 g and 3.78+0.48 g, respectively), hepatic (21.78+0.83 g and 23.15+0.37 g, respectively), or bursal (1.55+0.79 g and 1.63+0.21 g, respectively) body wei...
Tarek A. Wrshana1, Yousry A. Dowidar1, Bahgat A. El-Fiky2, Ali M. El-Rify1, Walaa A. El-Sayed3, Basem M. Ahmed4*
...uently emerges in highly vaccinated flocks causing high economic losses without specific treatment. Mesenchymal stem cells (MSCs) are a group of pluripotent cells with multiple biotechnology applications, including but not limited to tissue genesis, tissue repair, hematopoiesis, and immune modulation. Therapeutic strategies based on the usage of stem cells includes the cells either themselves or their secretions (secretome), which has recently shown ability to...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2
... higher titers in groups vaccinated with inactivated NDV genotype VII vaccines when compared with other groups. Regarding broiler performance parameters it was noticed that, there is no significant difference in body weight (BW) as well as feed conversion rate (FCR) among all chickens in different vaccinated groups. From these findings, we concluded that vaccination programs include ND vaccines genotypically- matched to chal...
Abdullah Iqbal1, Muhammad Abubakar2, Shumaila Manzoor3, Muhammad Kamran Ameen4 and Rani Faryal1*
...controls. All bucks were vaccinated with the Pestivac vaccine. Antibodies detection was done using competitive ELISA from serum samples. Antigen shedding by vaccinated animals via nasal and fecal route was checked by the haemagglutination test and RT-PCR. All group 2 bucks, developed humoral protection against PPR within the 1st week. Only, 50 percent bucks of group 1 developed humoral protection against PPR after one week o...
Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1
...culated. All chicks were vaccinated with live attenuated Newcastle Disease Virus LaSota strain on day 2 via eye drop vaccination, along with other basic immunization schemes. After 14 days of ND vaccination, post-vaccination sera samples were collected and subjected to an HI test to determine antibody titers. The Haemagglutination inhibition test was done using Newcastle Disease Virus LaSota strain NLT 106.0 EID50 antigen. Post-hatch feed and water deprivation...
Ayesha Bakhtiar1, Sardar Azhar Mehmood2, Abdul Rauf Bhatti3*, Shabir Ahmad2, Naqash Khalid2, Javed Iqbal5, Azra Nadeem4 and Waqas Ahmad1
...the broilers between the vaccinated groups, and the unvaccinated broilers (control group) performed better in terms of weight growth and FCR than the vaccinated groups. According to the data, group C of broilers given Lasota vaccine-2 proved to be more effective in terms of producing antibodies in broilers, while unvaccinated broilers performed better in...
Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie
..., died 10.269 heads, and vaccinated 5.847.113 heads. This article is carried out using literature study and secondary data exploration methods. There are three factors that can be attributed to the re-emergence of FMD outbreaks in Indonesia. First, the loss of the principle of maximum security. Indonesia’s success in freeing itself from FMD after 100 years is thanks to the implementation of maximum security policy by enacting a country-based system, whic...
M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4
...als from a safe farm not vaccinated against brucellosis. Determination of the sensitivity of the tested diagnostic tools was carried out using 46 blood serum samples from cows immunized with a vaccine from the B. abortus 82 strain from a farm that is unfavorable for brucellosis. Studies have shown that RNGA is one of the most sensitive tests, which allows to identify in a dysfunctional economy a high percentage of animals with brucellosis (39.1% of those studi...
Nazish Huma Khan1*, Muhammad Sufyan Khan1, Tooba Saeed2, Mohammad Ilyas3 and Hazrat Husaain4
...out 80% people have been vaccinated in the country till date. How much these vaccines are effective against COVID-19 disease is a question mark and is important to check its efficiency as it effects varies from person to person. Therefore, it is important to know the effectiveness of vaccines against COVID-19. For this, both medical and conventional methods need to be adopted depending on the severity of viral attack. Moreover, vaccination is also referred to ...
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
...). The first group was unvaccinated and kept as a control group all over the study while the animals in the second group were immunized with the prepared vaccine. Vaccinated cows received two vaccinal doses (5ml) at two week interval. The vaccine was injected subcutaneously in the brachiocephalic muscle. In experiment No. 2, the therapeutic potential of the prepared vaccine was tested in four groups of lactating cows sufferi...
Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3
... significantly higher in vaccinated bovine group as compared to non-vaccinated bovine with (12.93%), (9.45%) and (5.22%) respectively across the district. More specifically the cow population was carrying maximum seroprevalence of type O with (60.55%) compared to buffalo with (36.58%). It was concluded that serotype O was more prevalent in district Bahawalpur followed by serotype Asia 1 and serotype A and cow population was ...
Navjot Hothi
... getting their livestock vaccinated. Trends suggest that this disease could resurface in the summer of 2023 and could also spread across the nation, with the fear of becoming endemic. The virus can survive for months in dark enclosures. The main culprit behind the spread of the disease is indirect transmission via vector movement, which peaks during the summer and monsoon months. Direct transmission of the virus is found to be effectively low. Appropriate prec...
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...ositive and negative non-vaccinated controls. At 30 days of age groups A, B and C were challenged with VNDV genotype VII1.1 isolate, where the clinical manifestations, gross post-mortem lesions, Humoral immune response and also quantification of virus shed post-challenge (PC) via real-time QRT-PCR were all screened and precisely recorded. The results revealed that, group A chickens developed the highest humoral antibody titers throughout the vaccination schedu...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...es were collected from 8 vaccinated broiler flocks exhibited high mortality rates and bursal lesions in al-El-Fayoum province, Egypt during 2019 and 2020. Reverse transcription PCR was used to detect IBDV in clinical samples by amplification of the hyper variable region (HVR) of VP2 gene. Genetic analysis of the PCR amplicons has revealed that 3 samples belonged to very virulent IBDV (vvIBDV). The genotyping of Egyptian vvIBDv indicate progressive evolution co...
Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*
... higher survival rate in vaccinated mice and rabbits compared to placebo groups. None of the vaccines when streaked onto culture media showed any growth indicating sterility of the vaccines. Repeated measures ANOVA was applied to compare mean O.D values of treatment groups for evaluation of humoral immunity. Serum ELISA O.D values against S. aureus (tst), Str. uberis (cpn-60 STUB) and E. coli (aggR) in vaccinated groups rand...
Morcos Ibrahim Yanni1* and Eid Elsaid Abdelaziz2
...sures on imported rabies vaccinated pets in addition to prospective studies on the pathogenicity of local circulating isolates regarding the N and P genes are strongly recommended in the control strategy.
...
Hassanein, Suzan A.; Abd El-Wahab, Wafaa; Eweis, Moustafa and Mahmoud, Mervat M.
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
... disease virus (FMDV) in vaccinated animals. An indirect ELISA was established, for detection of the antibody response to FMDV NSP 3ABC using commercial ELISA kit (Prio-check) for l065 serum samples were collected (735) from Sharkia and (330) from Kafr ElSheikh Governorates during 2009 from cattle and buffaloes. The higher percentage of positive was detected in Sharkia (31.7%) while Kafr Elsheikh was (27.3%). The positive result of detection of antibodies agai...
Rizk, Sonia A.; El-garf, Eman M. and Talaat, Abeer A.
... and Mouth Disease (FMD) vaccinated calves. This study conducted in two calve groups; goup (A) vaccinated subcutaneously with bivalent Alhydragel adjuvanted (30) % FMD vaccine, while group (B) vaccinated subcutanously with bivalent FMD vaccine adjuvanted with both Alhydragel and GSLS (10 mg/dose). The humeral and cellular immunoresponses were monitored in different tested groups that recei...
Madbouly*, H.M.; Saif**, M.A. and Hussein* , A.S.
...) The Gl(control neither vaccinated nor treated), G2 vaccinated with NDV, G3 (vaccinated with MDV Rispen strain), G4 (vaccinated with MDV and NDV) , Group 5 vaccinated with MDV vaccine and treated with Curcuma Longa , and G 6 vaccinated with MDV, NDV and treated with Curcuma Longa . ...
Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.
...young turkey poults of unvaccinated flocks by passage on SPF-ECE of 5-7 days old embryos via yolk sac route. Characterization of isolated virus was done by using RT-PCR where the 5 isolates were positive for APV and produced a specific 315 bp products. Serological survey for APV antibodies in serum and egg yolk samples collected from non-vaccinated flocks (608 serum samples were obtained from broiler chickens from El-Fayoum,...
Mansour, Abeer E.M.; Khodeir, M.H. and Hussein, A.M.H.•
...n the immune response of vaccinated animals with FMD vaccine including such materials and their effect on the vaccine stability....
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K and Mona M.Aly
...the immune states of the vaccinated birds after application of H5Nl vaccine for the first time one month after the introduction of early outbreaks of 2006. Viral diagnosis was based on direct detection of viral RNA by real time PCR. Two positive cases from turkey and Grocer duck were processed for viral isolation and characterization. The level of antibodies was detected in vaccinated birds by HI test and the results were di...
Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi
...ples were collected from vaccinated chickens including some broiler breeders and commercial layers from the period Of December 2006 to February 2008 and tested for the immune response to the multiple vaccination with different H5Nl and H5N2 avian influenza vaccines (Reassortant H5N1 and 6 types of H5N2 vaccines) by hemaggtutination inhibition (HI) test using heterologous H5 antigen which represent non of the vaccine antigens. The samples were collected from 57...
Abeer, E.Mansour and Hegazi, A.Z.
...accine were estimated in vaccinated cattle, buffaloes, sheep, goats and camels. Five animals of each species were vaccinated using a dose of I ml for sheep and goats and 2 ml for cattle, buffaloes and camels inoculated subcutaneously. It was found that both of the immune parameters were generally increased gradually to reach the highest value of cellular immunity by the 2e day, while that of neutralizing antibodies was reach...
Attyat, M. Kotb; Abeer, E. Mansour; Naglaa, I. Aly; Zeinab, T.Salama and Azab, A. Mohamed
...ves, six month old, were vaccinated singly and simultaneously and two calves were left as none vaccinated control. The serum neutralizing antibody titer expressed in log10 of FMD (strain O and strain A) in calves vaccinated with bivalent FMD vaccine only were increased from (0.9 log10 and 0.9 log10) consequently at the first week post vaccination (WPV) till it reached (2.7 log10 and 2.85 l...
*Hanan M.M. Elzahed, Abd EL-Moneim A.A, *Afaf A. Khedr, *Elham A. Elabiare, Waleed S. Abd El-Latif, Reda M.I.
...riod indicating that the vaccinated hens were immunopotent. No sign of immunosuppression in progeny chicks was detected by measuring (NDV) HI antibody titers pre and post lasota vaccination and by vaccination challenge experiment, more over no (CAV) shedding was observed in progeny chicks at early stage of breeding period. In this stage CAV shedding was recorded to be most using CAV VPI PCR assay. These results support the evidence that vaccination of br...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
...ups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by measuring both cell-mediated and humoral immunity. The results of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good p...
Ashraf M. Metwally*, Ausama A. Yousif*k , Iman B. Walaa A. Attia M. Samy * , and Ismail M. Reda *
...IBD related mortality in vaccinated farms prompted this investigation into the genetic character of the circulating IBD virus (IBDV). Bursa and proventriculus samples were RT-PCR tested using novel primers flanking VP2 region coding the two major and two minor hydrophilic peaks. IBDV was detected in tested samples. Phylogenetic analysis of the sequenced PCR product and deduced amino acid sequences of IBDV Giza2008 VP2 demonstrated the continued circulation of ...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...immunity to RVF virus in vaccinated sheep has been determined by using both serum neutralization test (SNT) and enzyme-linked immunosorbent assay (ELISA) on sera collected weekly up to 7 weeks post vaccination, where the antibody titer elevated from I st WPV till reach the protective level at 5th WPV. Effective doses in mice has been calculated in mice by inoculation of 5 groups of mice (10 each) with 2 doses of five fold diluted vaccine; inoculated mice ...
Laila,A. Sedeek; Fatma,S. Mohamed and Manal Abo El-Yazyed
...arate group was left non-vaccinated and served as control. All animals remained clinically normal throughout the whole experiment period. A satisfactory humoral immune response to both virus vaccines in vaccinated animals was identified by SNT and ELISA. It was found that there is no difference in the immune response among different vaccinated groups and there is no drawbacks noticed from ...
M.A. Farag*, Abeer, E. Mansour* and s.M.
...immune response of sheep vaccinated with bivalent Foot and mouth disease (FMD) vaccine were studied. Significant decrease in humoral immune response against the vaccine strains 01/3/93 and A/l/Egypt/2006 accompanied by protection percentage of 33% against challenged virus 01/3/93 with low values of AOD were recorded in sheep previously infected with BVDV one week before vaccination. Challenging the immunity of sheep both simultaneously infected with BVDV and <...
Afaf, A. Khedr*; Hyam Farouk* and Taradi, A.Said. **
...n the immune response of vaccinated animals and the results showed that no significance differences in antibodies titer in all sheep groups which vaccinated either with Rev-I and attenuated RVF alone or simultaneously....
H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3
...rsal disease (IBD) among vaccinated chicken flocks during the period 2002-2003 revealed that all IBDV outbreaks occurred at 18-33 days of age. 70% (21/30) of these outbreaks characterized by typical clinical signs and gross lesions. The mortality rates in these flocks ranged between 9.4-12.8 % (average 11.1%); 16.4-30 % (average 23.2 %) and 12-23.5 % (average 17.75 %) in commercial broilers. commercial layer replacement pullets and native Baladi varietie...
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
...eks. The third group was vaccinated by I/P with 0.1 ml oil adjuvant vaccine (OAV) of Pasteurella multocida in two doses separated by two weeks. The fourth group (G4) was assigned the role of negative control at this time, the 28th day after immunization for work, serum samples were taken for passive hemagglutination test (PHA); Immunoglobulin G(IgG) detection using a chemical immunosorbent assay test. the outcomes of PHA test revealed that Folcord inhibited hu...
Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil
...ype VII.1.1 epidemics in vaccinated and non-vaccinated flocks are still recorded, indicating an urgent need to generate a new vaccine candidate. In this study, the live Orthoavulavirus javaense (AOAV-1) genotype VII.1.1 strain (CH-EGY-GIZA-VVTNRC-2021) (Genbank accession number MW603772) was attenuated by combining passaging with limiting 10-fold dilutions in the allantoic cavity of embryonated hens eggs for 20 passages. The...
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1
...creted in body fluids of vaccinated animals. In the present study, we constructed a more attenuated B. abortus ΔpurD mutant by deleting the purD gene from the RB51 strain through site-directed mutagenesis. For virulence attenuation comparison with the parent RB51 strain, the constructed mutant was evaluated for attenuation estimation and clearance in BALB/c mice. The B. abortus ΔpurD mutant exhibited significant attenuation of virulence and cleared...
Minh Thi Le Bui1, Thanh Minh Le2, Thuan Khanh Nguyen1*
...weeks. All chickens were vaccinated against Newcastle Disease on days 7, 21, and 60. Sixteen blood samples were taken from each treatment on days 49, 74, and 81. The HI test measured the serum antibody level against the Newcastle disease virus. The results showed that A. fistulosum L. powder supplementation (10 g/kg) in the basal diet increased the antibody titer against, while chlortetracycline (10 mg/kg) in the basal diet had a low antibody titer against ND ...
Aya A. Saber1, Mohamed Fawzy2*, MokhtarM. EL-Tarabili2, Eman K. El Sayed2
...; immune response as all vaccinated calves augmented acceptable levels of BEF and RVF specific antibodies. Therefore, calves can be vaccinated simultaneously against BEF and RVF safely with protective titers of antibodies....
Quart ul Ain1, Arfan Ahmad1*, Aftab Ahmad Anjum1 and Mamoona Chaudhry2
.... Groups A, B and C were vaccinated on 6th day of age with imported (Ivac), local (Lvac) and self-prepared (Spvac) vaccines, respectively. Group D (virus challenged) and E (non- vaccinated/non-challenged) were kept as control groups. Virus shedding in cloacal swabs, taken on 15 consecutive days PCI from all the groups (25th day, 100 EID50, intranasal) were tested through Real-Time RT-PCR. Humoral immune response were also me...
Klaus Euler and Zulfiqar Ali Qazi
...10% of the livestock was vaccinated. This came to a net saving of about 4.2 million rupees. While comparing it to the net income of Rs.730,000 - that the people of the Valley receive as revenue from timber-they are well off by a respectable margin of Rs.3.470 million rupees. If somehow, this message is made clear to the local people, a possibility exists that they might opt for better animal husbandry practices, than to go for timber....
Sundus Oun Ali Al-Zaini1, Mohanad A. Al-Bayati3*, Khazaal Abbas Khazaal2, Salma Talib Salih1
...viral and bacterial) was vaccinated once daily for fish along five days, dosed: 0.63g/fish/day and 1×109cfu/fish/day, respectively. The first two groups of fish were challenged by mingling them with KHV-infected fish at a ratio of two infected fish to ten vaccinated fish under simulated acclemtized normal condition. In contrast, fish from the second group were challenged with live Aeromonas hydrophila at a dose of (3&t...
Ibrahim Ali1,2, Hanan Mohamed Fathy Abdien1*, Wael Kamel Elfeil1, Mohsen Mohamed Zaky ElDemerdash1, Mohamed Ali Zain El-Abideen2,3, Walid Hamdy Kilany2,3
...ere treated as G1 and G2 vaccinated with (H5N8+Gel 01), G3 and G4 with (H5N8+IMS1313) by intranasal (IN) and intramuscular (IM) for each treatment respectively. Each group (G1-G4) was vaccinated twice at day 7 and 21 of age. Meanwhile, G5 treated with (H5N8+ISA70) received the primary vaccination dose intramuscularly only on day 7 of age. Group G6 served as a positive control (untreated and challenged), and G7 served as a ne...
Md Imran Hossen1, Shakhe Reju Ana Boishakhe1 and Mohammad Enamul Hoque Kayesh2*
...equently recorded in non-vaccinated young local male domestic cats. Anorectic condition, frequent vomiting, diarrhea, and different level of dehydration were recorded as the prominent clinical signs in FPV infection. Fluid therapy (5% dextrose saline and 0.9% saline), antiemetic (ondansetron), proton pump inhibitor (pantoprazole/esomeprazole), antibiotics (ceftriaxone or metronidazole and ceftriaxone) and multivitamins were suggested as the supportive treatmen...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
...ferentiate infected from vaccinated animals (DIVA) strategies. Therefore, the aim of this study was to detect LSDV in suspected clinically diseased cows from 6 herds in Al-Sharqia governorate, Egypt, between May 2021-April 2022. Moreover, to detect whether this infection is due to a field strain or vaccine strain based on partial sequence of the EEV Glycoprotein gene that firstly used in Egypt, for LSDV detection from 2 infected cows and three types of live at...
Hend E.M. Elsheikh1*, Mamdouh F. El-Mekkawi1, A.A. Abou-Zaid1 and Amal M. Abd El-Raof2
...ferentiate infected from vaccinated animals (DIVA) strategies. Therefore, the aim of this study was to detect LSDV in suspected clinically diseased cows from 6 herds in Al-Sharqia governorate, Egypt, between May 2021-April 2022. Moreover, to detect whether this infection is due to a field strain or vaccine strain based on partial sequence of the EEV Glycoprotein gene that firstly used in Egypt, for LSDV detection from 2 infected cows and three types of live at...
Tran Ngoc Bich1*, Vo Tuan Khai Huyen2, Nguyen Tran Phuoc Chien1, Dang Thi Tham1, Nguyen Vinh Trung1, Truong Van Hieu3, Thai Quoc Hieu4, Huynh Truong Giang1, Le Quang Trung1
...analysis revealed that unvaccinated animals had the highest incidence rate at 17.94% (P<0.05), with an OR of 5.52 (95%CI: 3.62–8.44). Additionally, calves under six months old have a significantly higher risk of LSD compared to cows aged 6–24 months (OR 1.84, 95%CI: 1.14–3.00) and compared to animals older than 24 months (OR 4.82, 95% CI: 2.73-8.51). Phylogenetic analysis of the P32 gene of LSDV (PP934191-PP934205) indicated 100% similarit...
Dalia Hamid Mansour1, Mohamed Elshabrawy Ghanem2, Marwa Hassan3, Yousry Ibrahim4, Ibrahim M. Hegab5*
... which chicks (n=35) was vaccinated and taken Biocid® for the entire experimental period (31 days) in both feed and drinking water. Group B, in which chicks (n=35) was vaccinated with no Biocid® supplementation. Group C, in which chicks (n=35) was not vaccinated but taken Biocid® only in feed. Birds were weekly weighted. Ten birds per group were slaughtered then blood samples w...
Nguyen Phi Bang1,2*, Nguyen Thi Hanh Chi1,2, Ngo Thuy Bao Tran1,2, Nguyen Thi Bich Hanh1,2, Nguyen Ba Trung1,2, Le Thi Thuy Hang1,2
.... The study surveyed 343 vaccinated dogs of different ages to determine the presence of antibodies in the serum and compare factors related to the dogs’ natural characteristics (age, gender) and other factors, such as dog management, regional characteristics, and vaccination timing. The study assessed the effectiveness of rabies vaccination in dogs by analyzing data from owners, health records, and serum samples. ELISA tests measured antibody levels, and...
Eslam Arafa1,2, Hanan M.F. Abdien1*, Mohamed Ali Zain El-Abideen3, Emad Diab2,4, Mahmoud Assad2,5, Mohamed Tarek3, Mohsen M.Z. El-Dimerdash1, Wael K. Elfeil1
...cks that originated from vaccinated breeders and were experiencing dwarfism, viral arthritis, and abnormal feathering. The full sequencing of the sigma C gene “partial S1 segment” from two samples indicated that they belong to variant ARV cluster 5, distinct from the vaccine strain, that showed only 41-47% amino acid similarity. The virus was isolated in specific pathogen-free embryonated chicken eggs (SPF-ECE) via the yolk sac and chorioallantoic ...
