Sayeda Sarah and Muhammad Ibrar

...ollowed by Glomus, Sclerocystis and Gigaspora. The average AMF spore density ranged from 56-260 spores/100g soil while root colonization ranged from 32-100%. Mycorrhizal enhancement regarding AMF spores density and root colonization ranked asRP0>RP1>RP2>RP3 in all hybrids i.e 0%>25%>50%>100%.

...

Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek

...on to control sugar beet cyst nematodes Heterodera schachtii. Lecanicillium muscarium decreased nematode population significantly (P=0.05 and caused reductions in final numbers of cysts per 250 g of soil, eggs and juveniles per cyst. Nematicides effects were not quite significant among each other but had tremendous effects in reducing nematode population compared to that of untreated contr...

Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1

...f mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stages and the number of goblet cells in the jejunal villi in the infected mice. Moreover, the expression of the goblet cell mucin gene, muc2 was upregulated after treatment with the root extract. Based on our ...

Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1

... hepatotoxicity. N-acetylcysteine (NAC) is the best antidote for APAP but may induce a variety of side effects. This study was designed to compare the potential impact of NAC with that of Thymoquinone (THQ), and/or Curcumin (CUR) either alone or in combination on liver injury induced by inflammation, oxidative stress in response to APAP toxicity in rats. Serum aminotransferases (ALT and AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total prote...

Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek

...ong other crops. It is a cyst-forming nematode that affects plant growth and yield. We report on studies aimed to evaluate the effects of five nematophagous fungi on the population dynamics of this pest in sugar beets in laboratory and greenhouse trials. The fungi chosen were Arthrobotrys oligospora, Dactylella oviparasitica, Clonostachys rosea, Stropharia rugosoannulata, and Lecanicillium muscarium. In the laboratory experiment, S. rugosoannulata proved to be...

Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2

...ts showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the BMI.

...
Wali Khan*, Ghazal Mumtaz, Saima Bibi and Salma Afzal
...nd Entamoeba histolytica cyst 28.5% were detected. The highest prevalence was recorded for Entamoeba histolytica while Trichuris trichura was detected as the least parasitic infection. Coriandrum annum (Coriander) was found highly contaminated 14.2% with parasitic infection while Zingiber officinale (Ginger) with least 1.78%. The vegetable collected from lower Dir were highly infected 41(73.2) than the upper Dir district 15(26.7). Based on the results of the p...

A. K. Ganguly and Uma Rao

Decades of researches in biochemical and molecular nematology at IARI
...y in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode interactions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya disease of wheat. Currently attempts are ...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Alaeldein M. Abudabos1,*, Abdullah H. Alyemni2, Elsayeid O. Swilam1 and Mu’ath Q. Al-Ghadi3
...irds. Lesion score and oocysts secretion increased significantly in the challenged birds, however, Elancoban had no significant difference with the natural products. It was concluded from the present study that natural products could be effective in comparison with anticoccidial drug to control coccidiosis in broiler chicken. 
...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali

...on period, addition of L-cysteine in co-cultivation medium, heat shock to immature embryos prior to infection, resting stage and Agrobacterium strains that deliver T-DNA into plant cell from which whole plant can be regenerated. The results indicated that optimal embryo physiological age is 15 and 18 days after pollination and transient transformation efficiency decreased at 22 days after pollination. Enhanced transient beta glucuronidase (GUS) expression with...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...lt in the development of cysts in the ovaries with resultant difficulties in reproduction. The present study was done on 75 rats divided randomly in 5 equal groups fed with rat chow, caged chicken meat, uncaged chicken meat, raw spinach and soybean for a period of six weeks. The levels of plasma cholesterol, progestril, estradiol, and androgens were estimated at the end of the experiment. The ovaries of the rats were collected, weighed and histopathologically ...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...ase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dysenteriae IM showed elevated alkaline-phosphatase, leucine a...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...ficant increase in blastocyst development when compared with other groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was significant...
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
...adenoma (FA), 81 of fibrocystic disease (FCD), 64 of breast abscess (BA), 12 of granulomatous mastitis (GM), 7 of lipoma, 5 of phylloides tumor (PL), 4 of fibrosis (F), 2 of intraductal papilloma (IDP) and 3 of accessory breast with Fibrocystic (FC) changes. The relation between the ages and frequency of the different types of lesions was also analyzed to understand the association between predisposing factors and the nature...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
... it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The crude enzyme was purified by ammonium sulfate precipitation method at 40 %. The molecular weight of the enzyme was found to be 33 kDa. The results showed that the enzyme produced from mutant strain gave significantly higher ...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
...d fluid filled abdominal cysts. Significant increase in intrauterine growth retardation and resorptions of fetuses, with increasing dose concentration, was also observed. Morphometric observations of fetal body parts like head circumference, eye circumference, forelimb and hindlimb size and tail length demonstrated a significant (P<0.005) decrease with increasing dose concentrations in comparison with control group. The fetal body weight and CR length reduc...

 Mian Azhar Ahmad1†*, Nawab Mohammad Khan2, Asmatullah3

Anatomical variations of hepatobiliary triangle in patients operated laparoscopically for gallbladder diseases from Lahore and Sahiwal
...st history of acute cholecystitis and obesity were subjected to Laparoscopic cholecystectomy. Patients’ age was between 18-70 years, gender ratio was 1:3.5 with female dominance, mean operative time 99.6 minutes and patients remained admitted for 1–3days. The procedure was performed successfully in 91% of the cases. In 09% cases, a conversion to standard open cholecystectomy w...

 Zafar Iqbal*, Hafiza Madhia Imtiaz

Parasites of double tail goldfish, Carassius auratus L. imported to Pakistan
...genean were also found encysted on gill filaments (infection 32%, mean intensity 3.87). Ichthyophthirius
multifiliis infection on fins was found 20% and mean intensity was found 2.4. Trichodina sp. infection on
fins was 12% (with mean intensity 13). Low infection by Piscinoodinium pillulare on fins (8%) and of
Tetrahymena sp. on fins and gills (4%) was also observed. This study has shown that imported double tail
goldfish was not he...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...d, and maternal hyperhomocysteinemia is an independent risk factor for CHD. In Saudi Arabia CHD represents one of the most important health problems. Here we examined the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and CHD and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

...te at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 ± 3.5% vs. 64.6 ± 1.8%, and 24.8 ± 2.2% vs. 15.9 ± 4.3%, respectively). However, blastocyst cell number was not different between groups. Furt...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspiration egg cytoplasm (P<0.05) on the cleavage rate, and was extremely significantly higher than Group III on the blastocyst rate (P<0.001). Raman spectroscopy of the spent culture media of these parthenogene...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...ate conditions, it forms cyst with high levels of resistance to disinfection methods. The aim of this study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that,
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...vorable application of L-cysteine for cryopreservation of Achai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 4...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1 

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

...erproduce high levels of cysteine and glutathione for enhanced stress tolerance especially against heavy metals. 

...
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
...ryptocaryon irritans cyst wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes and Genomes (...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...tated from tryptophan to cysteine. The results of this study have important reference significance for the future correlation analysis between MC1R gene and Nubian goat wool color traits and the color genetic mechanism of Nubian goats.
...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
... the presence of N-acetylcysteine (NAC). The renal mass of Wistar albino rats was reduced at a rate of 5/6. Captopril, Valsartan and NAC were applied intra-peritoneal alone or in combination. Blood pressure and heart rate were monitored at weekly intervals over a period of six weeks. Serum creatinine, blood urea nitrogen (BUN), lactate dehydrogenase (LDH) activity, cytokines (TNF-α, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both s...
Aouicha Djebroune1*, Aissa Mokabli1, Miloud Hammache2 and Gahdab Chakali2
...size: small;">The potato cyst nematodes (PCN) Globodera rostochiensis and Globodera pallida are considered as being redoubtable pests of potato crop and are subject to strict quarantine regulations in several countries. In Algeria, the potato crop is the most important food crop widely cultivated in different agricultural areas. In this investigation, the response of three potato varieties; Kondor, Spunta and Desiree to the evolution of populatio...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...2-cell embryos and blastocysts. The cytoplasmic maturation of MII oocytes and blastocysts in aged mice were significantly compromised to those of young mice (P < 0.05). Furthermore, GV oocytes, 2-cell embryos and blastocysts showed significantly higher relative intensities of pi-H2AX in aged mice (P <0.05). Taken together, our result indicate that aging disturbed oocyte maturation an...
Masroor Ellahi Babar*
...morphic regions includes cysteine nucleotide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expression of those protein which are nearest to the region of gene regulation and enhancer region. After observing taxas several distinct clads of camel breeds it was found that...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...lopment of corpus-luteum cysts, follicular ovarian cysts, delayed age at puberty, repeated estrous cycles, dystocia, abortions, dead fetuses and retention of the fetal membranes. OT administered exogenously or secreted endogenously, establishes in minutes the desired effects and metabolized to inactive products rapidly in the body. Irrespective of whether OT is ingested with milk or secreted into the milk, it is degraded rea...
Tabassum Yaseen1*, Shehzad Ahmad1, Khushnood Ur Rehman2, Fayaz Asad1, Abdul Waheed1, Rani Gul3, Hussain Gulab4 and Naveed Akhtar2
Arbuscular Mycorrhizal Fungal Spore Density and Root Colonization in Weeds of Carrot field at Charsadda, Pakistan
...or Acaulospora and Sclerocystis in the rhizospheric soil of Sonchus asper and Fumaria indica respectively. The root infection was also investigated which showed 20-85% root infection. The highest root colonization was recorded in Parthenium hysterophorus (39.67±11.02) whereas the lowest was found in Fumaria indica and Taraxacum officinale (6.33±5.51), (8.00±4.00) respectively. It is not necessary that the plants having high spore density w...

Shahid Ahmed1,2*, Huiqing Su1, Xuqian Sun1, Peng Feng1, Yongpan Chen1, Liang Yu1, Qian Liu1 and Heng Jian1

Performance of Cereal Varieties against Cereal Cyst Nematode (Heterodera avenae)
...oilborne pathogen cereal cyst nematode (CCN) Heterodera avenae is one of the biotic stress factors that directly affects the crop physiology from seed germination to crop maturity and limits the cereal production in different agro-ecologies of the world. In China, this pathogen is widespread in more than 20 provinces and infects the major cereal varieties and germplasm being adopted for cereal crop production annually. We tested number of cereal accessions to ...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...site eggs, larvae, and oocysts by simple faecal floatation and formalin – ether sedimentation technique. All large felines in the zoo were infected with gastrointestinal parasites. A total of six species of gastrointestinal parasites were recovered including four nematodes (Toxocara cati, Ancylostoma spp., Toxascaris leonina, and Oxyuris sp.), a cestode (Spirometra sp.), and a protozoan (Isospora sp.). Half (n=5/...

 Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*

...esions of keratin-filled cysts over extensive areas of the body. Two consanguineous families were enrolled from Basti Mochi Wala, Mouza Gulab Shah in Muzaffargarh District of Punjab (Pakistan) having multiple siblings suffering from alopecia. The aim of this study was to find out the genetic mutation(s) in hairless (HR) gene, if any, in the enrolled subjects. A questionnaire was filled for each subject on the sampling site in order to collect epidemiolo...

 Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2

Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2,  Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...he antioxidants agents(L-cysteine and vitamin E) were added into semen diluent and assessed the freeze-thawed semen quality parameters. The ejaculates were collected from two crossbred groups (Sahiwal × Holstein-Friesian), and (Achai × Jersey) and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concen...

Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*

...4.86% and 5.03%, dermoid cyst 2.47%, 2.78% and 2.66%, and knuckling of limb 2.33%, 2.36% and 2.10% in Fulbaria, Muktagacha, and Trishal Veterinary Hospitals, respectively. Different environmental factors and seasons influenced the prevalence of surgical affections. Heavy rainfall and high humidity have made this region a vulnerable place for the prevalence of surgical affections in calves. This study has correlated between different variables and the prevalenc...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2
Mark Wen Han Hiew1 and Lawan Adamu1
...each, ten ejaculates for cysteine in experiment 1 and the other ten for ascorbic acid groups in experiment 2. Each ejaculate from an individual horse was divided into six equal parts of the study, and they were cryopreserved using the same procedure. Different concentrations of either cysteine (0, 0.25, 0.5, 1, 2, and 4mg/ml) or ascorbic acid (0, 0.25, 0.5, 1, 2, and 4mg/ml) were added to the semen of the first and second ex...
Naimat Ullah Khan1*, Muhammad Hassan Saleem2, Aneela Zameer Durrani2, Nisar Ahmad2, Muhammad Shafee3, Ayesha Hassan2, Mumtaz Ali Khan2 and Nadeem Rashid3
...ths. Identification of oocysts was done through conventional acid fast ZN staining. Prevalence in District Bannu was 48/480 (10%) followed by District Lakki Marwat12.08 % and in Kohat 19.16 %, respectively. Overall prevalence in three Districts was 13.75%. The highest month wise percent prevalence was recorded during July/August 25.83%, 26.66% with lowest in December 0.83%. On seasonal basis overall highest prevalence was during the summer season 21.87 %, foll...
Xuhai Wang1,2, Xin Li1, Fangyuan Yuan1, Chaocheng Li1, Bin Jia1* and Song Jiang1*
...y regulated IL2RB in the cystic echinococcosis intestinal tissues, and they were differentially expressed in the intestine tissue between the resistant sheep and non-resistant sheep, more widely distributed in the resistant group. These results illustrated that miR-216b and IL2RB activated stronger immune response in the resistant sheep, compared with the non-resistant sheep, and participating in the T cell immune response.
...

Sagir Hussain*, Qamar Abbas, Abdur Razzaq and Sher Wali Khan

...altistan, Pakistan, four cyst nematode species viz., Heterodera avenae Wollenweber, 1924; H. mani Mathews,1971; H. schachtii Schmidt, 1871 and H. zeae Koshy, Swarup and Sethi, 1971 were detected from all four districts.

...

 Yanqiang Zhou1, Lixin Wang1, Chunxiao Hao1, Xiuyun Li2, Shakeel Hussain1, Dongdong Shen1, Zhiwei Peng1, Qi`an Zhai1 and Zhijun Hou1,*

...d on the morphology of oocysts, indicates that the present parasites are Capillaria sp. in blue wildebeest; Eimeria christenseni, E. alijevi, Trichuris sp. and one Strongy-type species in goats; Trichuris sp., Nematodirus sp., Moniezia sp., E. macusaniensis, another Eimeria sp. and another Strongy-type (different with goats) in alpacas. It was discovered that the infection rate was 45.74%, 38.97%, and 12....

Pakistan Journal of Zoology

October

Vol. 53, Iss. 5, Pages 1603-2000

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