Sayeda Sarah and Muhammad Ibrar
...ollowed by Glomus, Sclerocystis and Gigaspora. The average AMF spore density ranged from 56-260 spores/100g soil while root colonization ranged from 32-100%. Mycorrhizal enhancement regarding AMF spores density and root colonization ranked asRP0>RP1>RP2>RP3 in all hybrids i.e 0%>25%>50%>100%....
Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...on to control sugar beet cyst nematodes Heterodera schachtii. Lecanicillium muscarium decreased nematode population significantly (P=0.05 and caused reductions in final numbers of cysts per 250 g of soil, eggs and juveniles per cyst. Nematicides effects were not quite significant among each other but had tremendous effects in reducing nematode population compared to that of untreated contr...
Felwa A. Thagfan1, Mohamed A. Dkhil1,2* and Saleh Al-Quraishy1
...f mice with sporulated oocysts. A dose of 300 mg/kg reduced the number of oocyst output in mice faeces by about 56.8 % and also improved the body weight. In addition, the root extract decreased the number of parasitic stages and the number of goblet cells in the jejunal villi in the infected mice. Moreover, the expression of the goblet cell mucin gene, muc2 was upregulated after treatment with the root extract. Based on our ...
Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
... hepatotoxicity. N-acetylcysteine (NAC) is the best antidote for APAP but may induce a variety of side effects. This study was designed to compare the potential impact of NAC with that of Thymoquinone (THQ), and/or Curcumin (CUR) either alone or in combination on liver injury induced by inflammation, oxidative stress in response to APAP toxicity in rats. Serum aminotransferases (ALT and AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total prote...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
...ong other crops. It is a cyst-forming nematode that affects plant growth and yield. We report on studies aimed to evaluate the effects of five nematophagous fungi on the population dynamics of this pest in sugar beets in laboratory and greenhouse trials. The fungi chosen were Arthrobotrys oligospora, Dactylella oviparasitica, Clonostachys rosea, Stropharia rugosoannulata, and Lecanicillium muscarium. In the laboratory experiment, S. rugosoannulata proved to be...
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...ts showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the BMI....
Wali Khan*, Ghazal Mumtaz, Saima Bibi and Salma Afzal
...nd Entamoeba histolytica cyst 28.5% were detected. The highest prevalence was recorded for Entamoeba histolytica while Trichuris trichura was detected as the least parasitic infection. Coriandrum annum (Coriander) was found highly contaminated 14.2% with parasitic infection while Zingiber officinale (Ginger) with least 1.78%. The vegetable collected from lower Dir were highly infected 41(73.2) than the upper Dir district 15(26.7). Based on the results of the p...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...y in major root knot and cyst nematode species of India to assist the breeding program for developing resistant varieties and other purposes like regulatory and nematode diagnostics. Attempts have also been to made to understand the molecular signaling events during compatible plant nematode interactions by identifying the genes during the transition from preparasitic to parasitic stage in ear cockle nematode and molya disease of wheat. Currently attempts are ...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
...
Alaeldein M. Abudabos1,*, Abdullah H. Alyemni2, Elsayeid O. Swilam1 and Mu’ath Q. Al-Ghadi3
...irds. Lesion score and oocysts secretion increased significantly in the challenged birds, however, Elancoban had no significant difference with the natural products. It was concluded from the present study that natural products could be effective in comparison with anticoccidial drug to control coccidiosis in broiler chicken.
...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...on period, addition of L-cysteine in co-cultivation medium, heat shock to immature embryos prior to infection, resting stage and Agrobacterium strains that deliver T-DNA into plant cell from which whole plant can be regenerated. The results indicated that optimal embryo physiological age is 15 and 18 days after pollination and transient transformation efficiency decreased at 22 days after pollination. Enhanced transient beta glucuronidase (GUS) expression with...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...lt in the development of cysts in the ovaries with resultant difficulties in reproduction. The present study was done on 75 rats divided randomly in 5 equal groups fed with rat chow, caged chicken meat, uncaged chicken meat, raw spinach and soybean for a period of six weeks. The levels of plasma cholesterol, progestril, estradiol, and androgens were estimated at the end of the experiment. The ovaries of the rats were collected, weighed and histopathologically ...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...ase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover, S. dysenteriae IM showed elevated alkaline-phosphatase, leucine a...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...ficant increase in blastocyst development when compared with other groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was significant...
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
...adenoma (FA), 81 of fibrocystic disease (FCD), 64 of breast abscess (BA), 12 of granulomatous mastitis (GM), 7 of lipoma, 5 of phylloides tumor (PL), 4 of fibrosis (F), 2 of intraductal papilloma (IDP) and 3 of accessory breast with Fibrocystic (FC) changes. The relation between the ages and frequency of the different types of lesions was also analyzed to understand the association between predisposing factors and the nature...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
... it with low levels of L-cysteine HCl to protect it from instability. It was observed that under optimum conditions at 30°C temperature, pH 7, and incubation period 64 h, the lipase activity was increased up to 124±2.48 U/g. The crude enzyme was purified by ammonium sulfate precipitation method at 40 %. The molecular weight of the enzyme was found to be 33 kDa. The results showed that the enzyme produced from mutant strain gave significantly higher ...
Madeeha Arshad1, Asmatullah1, Chaman Ara1, Shagufta Andleeb2 and Naveed Ahmad3,*
...d fluid filled abdominal cysts. Significant increase in intrauterine growth retardation and resorptions of fetuses, with increasing dose concentration, was also observed. Morphometric observations of fetal body parts like head circumference, eye circumference, forelimb and hindlimb size and tail length demonstrated a significant (P<0.005) decrease with increasing dose concentrations in comparison with control group. The fetal body weight and CR length reduc...
Mian Azhar Ahmad1†*, Nawab Mohammad Khan2, Asmatullah3
Anatomical variations of hepatobiliary triangle in patients operated laparoscopically for gallbladder diseases from Lahore and Sahiwal
...st history of acute cholecystitis and obesity were subjected to Laparoscopic cholecystectomy. Patients’ age was between 18-70 years, gender ratio was 1:3.5 with female dominance, mean operative time 99.6 minutes and patients remained admitted for 1–3days. The procedure was performed successfully in 91% of the cases. In 09% cases, a conversion to standard open cholecystectomy w...
Zafar Iqbal*, Hafiza Madhia Imtiaz
Parasites of double tail goldfish, Carassius auratus L. imported to Pakistan
...genean were also found encysted on gill filaments (infection 32%, mean intensity 3.87). Ichthyophthirius
multifiliis infection on fins was found 20% and mean intensity was found 2.4. Trichodina sp. infection on
fins was 12% (with mean intensity 13). Low infection by Piscinoodinium pillulare on fins (8%) and of
Tetrahymena sp. on fins and gills (4%) was also observed. This study has shown that imported double tail
goldfish was not he...
multifiliis infection on fins was found 20% and mean intensity was found 2.4. Trichodina sp. infection on
fins was 12% (with mean intensity 13). Low infection by Piscinoodinium pillulare on fins (8%) and of
Tetrahymena sp. on fins and gills (4%) was also observed. This study has shown that imported double tail
goldfish was not he...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...d, and maternal hyperhomocysteinemia is an independent risk factor for CHD. In Saudi Arabia CHD represents one of the most important health problems. Here we examined the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and CHD and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
...te at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 ± 3.5% vs. 64.6 ± 1.8%, and 24.8 ± 2.2% vs. 15.9 ± 4.3%, respectively). However, blastocyst cell number was not different between groups. Furt...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspiration egg cytoplasm (P<0.05) on the cleavage rate, and was extremely significantly higher than Group III on the blastocyst rate (P<0.001). Raman spectroscopy of the spent culture media of these parthenogene...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...ate conditions, it forms cyst with high levels of resistance to disinfection methods. The aim of this study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that,
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...vorable application of L-cysteine for cryopreservation of Achai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 4...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
...erproduce high levels of cysteine and glutathione for enhanced stress tolerance especially against heavy metals. ...
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
...ryptocaryon irritans cyst wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes and Genomes (...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...tated from tryptophan to cysteine. The results of this study have important reference significance for the future correlation analysis between MC1R gene and Nubian goat wool color traits and the color genetic mechanism of Nubian goats.
...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
... the presence of N-acetylcysteine (NAC). The renal mass of Wistar albino rats was reduced at a rate of 5/6. Captopril, Valsartan and NAC were applied intra-peritoneal alone or in combination. Blood pressure and heart rate were monitored at weekly intervals over a period of six weeks. Serum creatinine, blood urea nitrogen (BUN), lactate dehydrogenase (LDH) activity, cytokines (TNF-α, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both s...
Aouicha Djebroune1*, Aissa Mokabli1, Miloud Hammache2 and Gahdab Chakali2
...size: small;">The potato cyst nematodes (PCN) Globodera rostochiensis and Globodera pallida are considered as being redoubtable pests of potato crop and are subject to strict quarantine regulations in several countries. In Algeria, the potato crop is the most important food crop widely cultivated in different agricultural areas. In this investigation, the response of three potato varieties; Kondor, Spunta and Desiree to the evolution of populatio...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...2-cell embryos and blastocysts. The cytoplasmic maturation of MII oocytes and blastocysts in aged mice were significantly compromised to those of young mice (P < 0.05). Furthermore, GV oocytes, 2-cell embryos and blastocysts showed significantly higher relative intensities of pi-H2AX in aged mice (P <0.05). Taken together, our result indicate that aging disturbed oocyte maturation an...
Masroor Ellahi Babar*
...morphic regions includes cysteine nucleotide deletion, AT bases insertion and transvertions (C>G; G>T). Deletion of A bases at c.1-1075 and hetrozygosity (CT) at the c1-1046 were analyzed along with some other SNPs are very important. These evidences points towards the change of expression of those protein which are nearest to the region of gene regulation and enhancer region. After observing taxas several distinct clads of camel breeds it was found that...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...lopment of corpus-luteum cysts, follicular ovarian cysts, delayed age at puberty, repeated estrous cycles, dystocia, abortions, dead fetuses and retention of the fetal membranes. OT administered exogenously or secreted endogenously, establishes in minutes the desired effects and metabolized to inactive products rapidly in the body. Irrespective of whether OT is ingested with milk or secreted into the milk, it is degraded rea...
Tabassum Yaseen1*, Shehzad Ahmad1, Khushnood Ur Rehman2, Fayaz Asad1, Abdul Waheed1, Rani Gul3, Hussain Gulab4 and Naveed Akhtar2
Arbuscular Mycorrhizal Fungal Spore Density and Root Colonization in Weeds of Carrot field at Charsadda, Pakistan
...or Acaulospora and Sclerocystis in the rhizospheric soil of Sonchus asper and Fumaria indica respectively. The root infection was also investigated which showed 20-85% root infection. The highest root colonization was recorded in Parthenium hysterophorus (39.67±11.02) whereas the lowest was found in Fumaria indica and Taraxacum officinale (6.33±5.51), (8.00±4.00) respectively. It is not necessary that the plants having high spore density w...
Shahid Ahmed1,2*, Huiqing Su1, Xuqian Sun1, Peng Feng1, Yongpan Chen1, Liang Yu1, Qian Liu1 and Heng Jian1
Performance of Cereal Varieties against Cereal Cyst Nematode (Heterodera avenae)
...oilborne pathogen cereal cyst nematode (CCN) Heterodera avenae is one of the biotic stress factors that directly affects the crop physiology from seed germination to crop maturity and limits the cereal production in different agro-ecologies of the world. In China, this pathogen is widespread in more than 20 provinces and infects the major cereal varieties and germplasm being adopted for cereal crop production annually. We tested number of cereal accessions to ...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...site eggs, larvae, and oocysts by simple faecal floatation and formalin – ether sedimentation technique. All large felines in the zoo were infected with gastrointestinal parasites. A total of six species of gastrointestinal parasites were recovered including four nematodes (Toxocara cati, Ancylostoma spp., Toxascaris leonina, and Oxyuris sp.), a cestode (Spirometra sp.), and a protozoan (Isospora sp.). Half (n=5/...
Muhammad Ajmal1, Saima Mustafa1, Fizza Ibrahim Bajwa1, Cheng Zhou2, Guangdong Wen2, Soe Lwin Myint2, Syed Irfan Raza3, Ihtasham Bukhari4, Mubashir Hassan5, Muhammad Faisal6 and Furhan Iqbal1*
...esions of keratin-filled cysts over extensive areas of the body. Two consanguineous families were enrolled from Basti Mochi Wala, Mouza Gulab Shah in Muzaffargarh District of Punjab (Pakistan) having multiple siblings suffering from alopecia. The aim of this study was to find out the genetic mutation(s) in hairless (HR) gene, if any, in the enrolled subjects. A questionnaire was filled for each subject on the sampling site in order to collect epidemiolo...
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...he antioxidants agents(L-cysteine and vitamin E) were added into semen diluent and assessed the freeze-thawed semen quality parameters. The ejaculates were collected from two crossbred groups (Sahiwal × Holstein-Friesian), and (Achai × Jersey) and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concen...
Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*
...4.86% and 5.03%, dermoid cyst 2.47%, 2.78% and 2.66%, and knuckling of limb 2.33%, 2.36% and 2.10% in Fulbaria, Muktagacha, and Trishal Veterinary Hospitals, respectively. Different environmental factors and seasons influenced the prevalence of surgical affections. Heavy rainfall and high humidity have made this region a vulnerable place for the prevalence of surgical affections in calves. This study has correlated between different variables and the prevalenc...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...each, ten ejaculates for cysteine in experiment 1 and the other ten for ascorbic acid groups in experiment 2. Each ejaculate from an individual horse was divided into six equal parts of the study, and they were cryopreserved using the same procedure. Different concentrations of either cysteine (0, 0.25, 0.5, 1, 2, and 4mg/ml) or ascorbic acid (0, 0.25, 0.5, 1, 2, and 4mg/ml) were added to the semen of the first and second ex...
Naimat Ullah Khan1*, Muhammad Hassan Saleem2, Aneela Zameer Durrani2, Nisar Ahmad2, Muhammad Shafee3, Ayesha Hassan2, Mumtaz Ali Khan2 and Nadeem Rashid3
...ths. Identification of oocysts was done through conventional acid fast ZN staining. Prevalence in District Bannu was 48/480 (10%) followed by District Lakki Marwat12.08 % and in Kohat 19.16 %, respectively. Overall prevalence in three Districts was 13.75%. The highest month wise percent prevalence was recorded during July/August 25.83%, 26.66% with lowest in December 0.83%. On seasonal basis overall highest prevalence was during the summer season 21.87 %, foll...
Xuhai Wang1,2, Xin Li1, Fangyuan Yuan1, Chaocheng Li1, Bin Jia1* and Song Jiang1*
...y regulated IL2RB in the cystic echinococcosis intestinal tissues, and they were differentially expressed in the intestine tissue between the resistant sheep and non-resistant sheep, more widely distributed in the resistant group. These results illustrated that miR-216b and IL2RB activated stronger immune response in the resistant sheep, compared with the non-resistant sheep, and participating in the T cell immune response.
...
Sagir Hussain*, Qamar Abbas, Abdur Razzaq and Sher Wali Khan
...altistan, Pakistan, four cyst nematode species viz., Heterodera avenae Wollenweber, 1924; H. mani Mathews,1971; H. schachtii Schmidt, 1871 and H. zeae Koshy, Swarup and Sethi, 1971 were detected from all four districts....
Yanqiang Zhou1, Lixin Wang1, Chunxiao Hao1, Xiuyun Li2, Shakeel Hussain1, Dongdong Shen1, Zhiwei Peng1, Qi`an Zhai1 and Zhijun Hou1,*
...d on the morphology of oocysts, indicates that the present parasites are Capillaria sp. in blue wildebeest; Eimeria christenseni, E. alijevi, Trichuris sp. and one Strongy-type species in goats; Trichuris sp., Nematodirus sp., Moniezia sp., E. macusaniensis, another Eimeria sp. and another Strongy-type (different with goats) in alpacas. It was discovered that the infection rate was 45.74%, 38.97%, and 12....
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...nd elevated in the blastocysts stage. The following western blot analysis revealed a similar expression pattern with QRT-PCR and immunofluorescence. In conclusion, the expression pattern of Gas6 gene is closely related to oocytes maturation and embryo development. Gas6 may play a crucial role in the process of oocytes maturation and improve the development potential of early embryos by promoting the cytoplasmic maturation.
...
Oluwatoyin Adenike Fabiyi
...atodes most notably, the cyst nematode Heterodera sacchari. Broadly, synthetic nematicides are utilized in the suppression of soil nematode infestation of sugarcane, with positive outcome in yield. However, emergence of resistant nematode strains and health hazards are associated with the ceaseless use of the synthetics. The efficacy of furfural (2-furanaldehyde) from agricultural biomass waste was examined as a practicable replacement to the regular synthetic...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
..., 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony formation, flow cytometry to measure apoptosis, and quantitative real-time polymerase chain reaction (qRT-PCR) to assay LINC00857 and miR-340 expression. Bioinformatics and dual luciferase reports analyzed the targeting relationship be...
Mohammed Naji Ahmed Odhah1,2*, Dhary Alewy Almashhadany6, Abdullah Garallah Otaifah7, Bashiru Garba5, Najeeb Mohammed Salah2, Faez Firdaus Abdullah Jesse4*, Mohd Azam Khan G.K3
...the fertility of hydatid cysts among some ruminants (both local and imported breeds of cattle, sheep, and goats) slaughtered in the abattoirs in Dhamar Province-Yemen. The samples were collected from the Central Corporation Slaughterhouse in Dhamar-Yemen from January 2019 to November 2020. During this period, a total of 29,071 and 5,705 samples were collected from local and imported food animals respectively. The results of analysed samples revealed that the d...
Shireen S. Aboelwafa¹, Alsagher O. Ali², Rania Hamada³, Hassan Y.A.H. Mahmoud2*
... gondii and N. caninum oocysts. In addition, in developing countries such as Egypt, where sheep and goat meat is commonly consumed, T. gondii and N. caninum infection in small ruminants may also affect public health risks. So that we estimate the prevalence of T. gondii and N. caninum infections in Egyptian small ruminants, by using enzyme-linked immunosorbent assay (ELISA) to assess the seroprevalence in 92 sheep and 92 goats from four districts in Luxor gove...
Jing Fu
...to four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82±9.26, n=55), mucinous cystadenocarcinoma group (age 62.81±6.28, n=52) Group and normal ovarian group (age 62.71±7.97, n=160). All cases were examined by two gynecological pathologists. The expression levels of SATB2 and CK20 in differ...
Mian Abdul Hafeez1*, Adeel Sattar2, Faiza Aslam3, Muhammad Imran3, Kamran Ashraf1, Rashid Zia2 and M. Muntazir Mehdi1
...,000-70,000 sporulated oocysts of mixed species local isolates at 21st day of age. Groups A and B were medicated with Ibuprofen (100mg/kg body weight) orally and Ibuprofen (100mg/kg B.W) orally plus Clopidol (0.5g/kg) of feed respectively, post infection for five days. The group C was medicated with Clopidol (0.5g/kg) of feed. The group D served as infected unmedicated (negative) control and birds in group E were kept as noninfected and nonmedicated (positive)...
Chandrakala Rana1*, Deepak Subedi1*, Shanti Kunwar1, Rajesh Neupane1, Birendra Shrestha2, Khan Sharun3, Dinesh Kumar Singh4 and Krishna Kaphle2
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
...
Parthenogenetic blastocysts as a source of parthenogenetic embryonic stem cells (pESC) has low level of blastocyst rate and quality. CHIR99021 is a material that can increase the formation and quality of blastocysts in buffalo through inhibition of glycogen synthase kinase 3. The purpose of this study was to increase the blastocyst rate and quality of...
Erum Iqbal*, Firoza Kazi and Saboohi Raza
...ode parasites are cereal cyst nematodes, root-knot nematodes, lesion nematodes, seed-gall nematodes and stem nematodes. In this review article status of global wheat production is presented and its associated parasitic nematodes have been discussed with reference to chronological research data related to Pakistan....
M. Fatmawati1, L.T. Suwanti2*, Mufasirin3
...y ingesting sporulated oocysts in the environment, while humans can be infected by T. gondii due to environmental contamination by T. gondii oocysts, consumption of foods and milk containing cysts or tachyzoites of T. gondii. Raw milk is a risk factor for toxoplasmosis in humans. This paper discusses the presence of T. gondii in raw milk from various animals in both artificial and natural ...
Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6
...productive disorders and cystic ovarian disease (COD) is one of the important disease which cause major economic losses to farmers. COD affects fertility of animal which is important to reproduce young ones, that occurs due to negative impact factors on hypothalamus-pituitary stalkand normal function of the ovarywhich leads to alteration in follicular development, ovulation, reduced reproductive performance, unsuccessful ovulation, increased interval between p...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
... Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM ...
Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2
...ows, Cytokines, N-acetyl-cysteine, Reproduction
...
Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*
...T. spiralis) and Hydatid cysts. The coefficients of the inter- and intraassay variations in the repeatability tests were lower than 10%. The established method and the indirect hemagglutination (IHA) test were used to detect 50 clinical samples at the same time. The positive coincidence rate of the two was 94.11%. Therefore, the newly established indirect ELISA method had high sensitivity, specificity, stability, and intra- and interbatch repeatability. It cou...
Muhammad Kashif Maan1*, Rohma Shabbir2
...bital involvement of the cyst is very rare accounts only 1% percent of the hydatidsis in veterinary patients. A 2-year ram was presented to Surgery teaching hospital of University of Veterinary and Animal Sciences, Lahore with the complaint of progressive exophthalmos with proptosis without pain, disturbance in ocular motility, visual deterioration, and chemosis. There was no evidence of presence of cyst in lungs and liver u...
Jiacheng Du and Yuping Cao*
...in patients with the polycystic ovarian syndrome (PCOS) who underwent Invitro fertilization-embryo transfer (IVF-ET). This study selected 200 patients with PCOS who took IVF-ET in our hospital from January 2016 to January 2020 as subjects. The study subjects were divided into group A (low-level group, n=90) and group B (high-level group, n=110) according to serum AMH levels; and group C (successful group, n= 88) and group D (failure group, n=112) according to ...
Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3
...cancer (BC; n = 4), fibrocystic mastopathy (FCM; n = 3) and healthy cats (n = 4), the following were examined: (1) haematological parameters; (2) biochemical blood indicators; (3) expression of ifnα7, ifnβ1, ifnγ, ifnλ1, p53, rb1, cdkn2a and gadd45g genes in the BC and FCM tumor tissues, as well in the uterus tissue and WBCs using qPCR. Results. In cats with MG tumours, signs of lymphopenia and thrombocytopenia were found, these being e...
Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1*
...hicken Eimeria species oocysts. The immature oocysts were exposed to 5, 10, 20, and 40 ppm, each for 1, 3, 6, 9, 12, 24, and 36 h. Percentage of sporulation inhibition in oocysts and comet assay were used to investigate the C-Oo.Nc destructive effects. The infectivity of sporulated oocysts exposed to LC50 & LC90 were evaluated by In vivo inoculation ...
Zheng-Yong Wen1, 2*, Chuan-Jie Qin1,2, Bin Li1,2, Rui Li1,2 and Xiao-Tao Shi3*
... a-helix domains and two cysteine residues were conserved in vertebrates. Three-dimensional (3D) structure modeling revealed that pvLeptin was highly conserved with that of other tetrapods. Genetic synteny analysis revealed that lepB had specifically lost in siluriformes teleosts. Phylogenetic analysis showed that fish lineage contained two clades of leptinA and leptinB, and the pvleptin was grouped into leptinA clade and shared a close relationship with its c...
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
...is expression in hepatic cystic echinococcosis. Experimentally hydatid cysts infection of BALB/c mice was established, and the manifestation of immune actions and apoptosis were detected histopathologically and by immunohistochemical markers staining. The infected livers with hydatid cysts from the mice were processed for routine paraffin block technique. The sections of infected livers th...
Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4
...s in SD rat model of polycystic ovary syndrome (PCOS) induced by DHEA, and to explore the possible mechanism. Forty rats were randomly divided into four group, the model groups each of 10 the control group, the DHEA group (low dose, 15 μg/kg/d) and the DHEA group (high dose, 30 μg/kg/d). DHEA subcutaneous injection was used to construct the PCOS model of the polycystic ovary syndrome (PCOS) model of rats. The general c...
Abdurakhim E. Kuchboev1*, Mehmonjon Kh. Egamberdiyev2
Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1
...rosis and sub-epithelial cyst formation. Histopathology in both treated groups showed the development of a complete layer of epithelium within 1week which increased in rows at 2nd and 3rd weeks of treatment and the stromal edema decreased with time unlike control groups that showed weak fragmented epithelium development, sub-epithelial hemorrhage and vascularization. These findings prove that EVs have the ability to heal corneal wounds even if in the periphera...
S. Ahmed†, Q. Liu and H. Jian
...fested Heterodera avenae cysts and screened against the second stage juvenile
(J2) mortality assay in vitro. About 40.16 % isolates showed 40.0% J2 mortality during 12 hours. Significantly
increased upto 86.0% mortality was observed in 24 hour exposures. Among them twenty six Bacillus strains were
identified by morphological, physiological and biochemical carbon sourc...
I. K.A. Ibrahim1, Z.A. Handoo2† and A. B. A. Basyony1
...ted for the detection of cyst nematodes. The results showed
the prevalence of nine cyst nematode species associated with different crop plants: Heterodera avenae on wheat, H.
daverti and H. trifolii on Egyptian clover, H. leuceilyma on Bermuda grass, H. lespedezae on lentil, H. goldeni on
qasabagrass, H. schachtii on cabbage and sugar beet, H. zeae on corn and wheat and Globodera rostochie...
Öznur Özil*, Öznur Diler, Mevlüt Nazıroğlu, Aşkın Atabay
... the infection was 27.14 cysts per host, varying between 1-75 cysts. The parasites were determined encysted in the base of the fins, muscle, inner wall of the operculum, gill arches, lips, upper jaw, body cavity and palate, forming small 2-3 mm diameter white-yellowish nodules, easy to detect in macroscopical observation. The highest prevalence of the metacercariae was in C. complanatum wi...
I. K. A. Ibrahim1 and Z. A. Handoo2†
...ernorate, the sugar beet cyst nematode
(Heterodera schachtii) was very common on sugar beet while the root-knot nematodes Meloidogyne incognita and
M. javanica were very common on guava, olive trees and sugar beet. Helicotylenchus pseudorobustus, M. incognita,
Pratylenchus sp., Rotylenchulus reniformis and Xiphinema sp. were observed in spearmint soil samples. The dagger
nematode Xiph...
I. K. A. Ibrahim1, M. A. EL-Saedy2, S. F. A. Awd-Allah3 and Z. A. Handoo4, †
...>The interactions of the cyst nematodes Heterodera daverti, H. goldeni and H. zeae with the root-knot nematode
Meloidogyne incognita on rice (Oryza sativa) cultivars 'Giza 178' and Sakha 101' were studied in the greenhouse.
Inoculation with H. goldeni alone or one week before inoculation with M. incognita on rice cv. Sakha 101
resulted in a significant increase in the number of cysts of H....
Y. I. Erum and F. Shahina†
... response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA
(RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum
percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and
molecular characterization that the most diverse and resistant genotype against CCN was Moomal-200...
S. Fatemy†1 and H. Ghasemi2
...nvestigated. Disinfected cysts of G. rostochiensis were placed in root diffusates and the numbers of hatched second stage juveniles (J2) were counted weekly over a 6-week period. Pots were planted with a single sprout of each cv. and the soil was inoculated with a suspension of 400 freshly hatched J2. There was a significant difference in the numbers of J2 that had entered the roots as well as a delay of 1-2 weeks in the rate of development of juveniles in the...
S. Ahmed†, Q. Liu and H. Jian
...tage juveniles of cereal cyst nematode, Heterodera avenae. The isolate XZ33-3 showed significant results against juveniles (J2s) mortality followed by the isolate of XZ 17-1, XZ 24-2-1, MH 58-60-10, MH 58-60-04 and isolate MH 01-04-01, respectively. Among them four Bacillus isolates were identified on molecular basis using 16S rDNA, physiologically and biochemically and grouped as plant growth promoting bacteria (PGPB). In the green house experiments identifie...
A.E. Ismail† and A.M. Kheir*
...ynamics of juveniles and cysts of corn cyst nematode, Heterodera zeae under stress of a cropping
sequence regime revealed that the average numbers in each stage greatly fluctuated. The density of juveniles or
cysts gradually increased with the favorable host (Giza 2 corn) to reach its peak at the crop maturity, while the
nema...
Laveena Shekhawat, Sheethal S*
...
Introduction: Hydatid cyst is a parasitic infection caused by Echinococcus granulosus commonly termed as dog tapeworm. However, man is an accidental host and develops symptoms of the disease in organs where the eggs get lodged, the most common being the liver. Case report: A 29-year-old male presented with pain in the right upper quadrant with radiation towards the back along with abdominal fullness for 5-7 days. Physical examination and imaging investigati...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...earch study investigated cysteine supplements and their effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml ...
Seyedmohammad Karimi1, Mohammad Darvishi2*, Mohammad Barati3, Ramin Hamidi Frahani4, Mohammad Hassan Kazemi5
...r. Melatonin and N-acetylcysteine, two drugs that have previously been shown to protect the liver with their antioxidant properties, may reduce the hepatic toxicity induced by Remdesivir. Given that few studies have been performed on the role of oral melatonin and N-acetylcysteine on reducing the hepatic adverse effects of Remdesivir, we decided to conduct this clinical trial study. In this double-blind, randomized clinical ...
Anatoly Murashevich Bittirov1*, Sadrutdin Shamshitovich Kabardiev1, Ayub Yusupovich Aliev1, Boris Kazievich Laipanov2, Svetlana Aleksandrovna Shemyakova2, Ismail Anatolyevich Bittirov1
Saima Akter1, Md. Rasel Prank1, Shariful Islam1, Sharmin Akter1, Injamamul Hasnine1, Murshed Uddin Ahmed2, Md. Shohel Al Faruk3*
...resence of the egg and oocyst of parasites. The overall prevalence of cattle parasitic infestation (mixed and single) was 80.17% (N=97/121). The overall prevalence of trematode was 49.58%, nematode was 57.86%, and protozoa was 9.1%. The infestation rate of the Strongylys group (45.4%) was dominant followed by Fasciola gigantica (24.79%), Paramphistomum spp. (24.79%), Neoascaris spp. (12.4%) and Eimeria (9.09 %). The parasitic infestation in cows was higher tha...
Zubair Ali, Muhammad Sohail*, Yasir Ameen, Hamidullah, Ishtiaq Ahmed and Mehwish Malik
... nymphomenia, follicular cysts, Luteal cysts, Buserelin 0.0105 mg/lecirelin 50µg and PGF2α Analogue100µg Intramuscular (IM) were used. Further research on the efficacy of protocols that integrate ultrasonography with timed AI protocols for re-synchronzation of ovulation, differential management strategies for cows carrying twin fetuses conducted....
Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi
...in Baghdad city, Iraq. Oocysts were isolated from naturally infected quails identified as Cryptosporidium meleagridis and used as a strain in experimental infection, about fifty birds were divided into five groups (G1: control negative group (con-), G2: control positive group (con+), G3: infected and treated with paromomycin, G4: infected and treated with curcumin and G5: uninfected and received curcumin). The oocyst sheddin...
Pengliang Xie1, Lufang Zheng2*, Mingxia Dong3, Huaiqiang Zhang1 and Fang Chen1
...r edema, and 14 cases of cystoid macular edema. Forty-five healthy people in physical examination were recruited as control. We found that the diseased group had higher proportion of hypertension (P<0.05) compared with the control group, and there was no difference in other general information (gender, age, BMI) (P>0.05). Compared with the control group, the diseased group had higher expression of sVEGFR-1 and sVEGFR-2 mRNA (P<0.05), and higher conten...
Kétomon Pierre Challaton1*, Coovi Guénolé Akouedegni1, Kadoéito Cyrille Boko2, Goué Géorcelin Alowanou1,3, Pascal Venant Houndonougbo4, Aboudou Habirou Kifouly1, Mawulé Sylvie Hounzangbé-Adoté1
...Gram of feces (EPG) or Oocysts Per Gram of feces (OPG) were higher in the wet season than in the dry season. During the wet season, infestations were severe for strongyles, Moniezia spp., coccidian, and moderate for Strongyloides spp. and light during the dry season except for coccidia, where they were intense. Infestation rates and egg excretion of gastrointestinal parasites were shaped by age, sex, breed, and study areas. This knowledge of gastrointestinal ...
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...CS for eight days. Blastocyst stages from each treatment group were assessed and evaluated for their quality by determining the ACR by means of TUNEL staining. The presence of palmitic or stearic acid at high concentrations during oocyte maturation increased ACR, whereas oleic acid had no significant effects. The results of the present study concluded that compromised oocyte microenvironment by metabolic stressor such as high NEFAs concentrations which is the ...
Ahmed El-Sayed Ismail
...he virulence of the corn cyst nematode, Heterodera zeae such as corn genotype, corn age, corn nutritional status, and cropping sequence regime. For the first factor, when two local corn cultivars, namely, Giza 2 (susceptible) and Kahera 1 (resistant), were subjected to the infection by H. zeae to determine the biotic effect of the host susceptibility on development and reproduction of the nematode, the final population and rate of build-up of the nematode were...
Amna Mushtaq, Asia Bibi* and Nahid Kausar
...al manifestations of polycystic ovarian syndrome (PCOS) have been linked with psychotic morbidity and infertility in the women of fertile age. Considering limited research data available about the psychological aspects in these patients, we conducted current study to assess psychotic distress and infertility in PCOS patients of southern Punjab, Pakistan. In this study 204 PCOS women and 150 controls were enrolled. Data of recruited patients was collected from ...
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
... morula XY embryo, blastocyst XX embryo, and blastocyst XY embryo. A sequencing library was constructed by the Smart-Seq2 amplification. The transcriptome was sequenced by Illumina HiSeqXten high-throughput sequence technology, and effective sequences were analyzed by functional annotation and related bioinformatics analysis. We found that Q30 percentage range of eight samples was 91.79-92.37%. The filtration sequence was 44...
Muhammad Nasir Bhaya1,2* and Hikmet Keles2
... degeneration, swelling, cystic dilatation and luminal proteinaceous material in tubuli, congested and hypercellular glomeruli and also inflammatory cells infiltration in the cortical and medullary area in CP group. Significant decrease of pathological lesions was found in CP+EA groups especially in the CP+EA75 group. In immunohistochemical sections, 8-hydroxy-2-deoxyguanosine (8-OHdG) and hypoxia-inducible factor-1 alpha (HIF-1α) revealed less positivit...
Nida Sadaqat1, Sheheyar Ahmed Khan1, Amna Bibi1, Sadaf Zahra1, Muhammad faisal Salamt1, Noreen Latief2 and Fatima Ali1*
...e the effect of N-acetyl cysteine (NAC) on biochemical alterations during the post-burn stage in scalded rats. Twenty-four male Sprague-Dawley rats were anesthetized and dorsum was shaved. Second-degree skin burn was induced by immersing an iron mold (1.0 cm2 in diameter) in boiling (100°C) water for 5 min and placed on the back of the rats for 20 sec without applying pressure. Rats were divided into three groups; control group, burn injury group, burn inj...
Fatima Ali1*, Rimsha Tahir1 and Tahir Rehman Samiullah2
...ombination with N-acetyl cysteine (NAC) for the treatment of excisional wounds in rats. Sprague Dawley (SD) rats were inflicted with excisional wounds. Wounds were treated with honey as control group and in combinations with 1%, 3% and 5% NAC. Wound area was measured after 3rd, 7th, 10th and 14th day. Improvement in wound healing was also confirmed by analyzing angiogenesis, apoptosis and chemotaxis genes. A significant increase was observed in the percentage ...
Waqas Alam1, Sar Zamin Khan1 and Rifat Ullah Khan2*
Fariha Qahar and Muhammad Sayyar Khan*
...rate-limiting enzyme for cysteine (Cys), and GSH biosynthesis, were subjected to H2O2, metolachlor, and atrazine-induced oxidative stress. The overexpression of the NtSAT4 gene from Nicotiana tobacco under 35S promoters in various compartments of the cell, which includes cytosol, plastids, and mitochondria in transgenic lines, resulted in enhanced tolerance in terms of lesser wilting and pigment discoloration to induced stress compared to non-transformed plant...
Syed Shakeel Shah1, Ayesha Jameel1, Sabila Afzal1*, Muhammad Zubair2, Iram Fatima Bokhari1
... recover parasites eggs, cysts and larvae. A high prevalence of about 47.58% was described in this study. Coriander was the highest contaminated vegetable (51.42%), followed by mint (48.57%). The carrot was the minimum contaminated vegetable with 40% contamination. Examination of vegetables revealed 12 genera of parasites along with aquatic mites (5.74%). Taenia was the most prevalent parasite (25.28%) followed by Ascaris (21.83%), Toxocara (9.19%) and Hymenol...
Hui Fang*, Kai Xue and Teng Yang
...o autosomal dominant polycystic kidney disease (ADPKD). Fifty RNA samples from Gene Expression Omnibus (GEO) database were obtained to identify differentially expressed miRNAs (DEMs) and genes (DEGs) in the progression of ADPKD. Functional annotation and enrichment analysis of DEG were performed. iRegulon was used to construct the miRNA-mRNA network and predict related transcription factors (TFs) associated with ADPKD. The function of miRNA network was explore...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...in is a thiol-containing cysteine protease isolated from the stem, peel, and leaf parts of Ananas comosus. Protease accounts for 60% of the total enzyme market. Being a plant-based cysteine protease, it has various pharmaceutical and biotechnological applications. The isolation and purification cost of the enzyme is highest, so there is a need to develop cost-effective purification methods. This research work aims to purify ...
Haneen Imad Al-Sultani1*, Ahmed Obaid Hussain1, Hazar Shakir Saleh2
...dney function, including cystatin C and vitamin D3. Then, the rats were dissected, and kidney tissues were taken for histological study. Male rats treated with rosuvastatin showed an elevated cystatin C level and increased vitamin D3. At the same time, the results of the microscopic examination showed significant histopathological changes in the kidneys of the groups treated with rosuvastatin compared to the control gro...
Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*
...ext-align: justify;">Polycystic ovarian syndrome (PCOS) is among the common endocrine disorders in childbearing aged females. This study is designed to compare a few anthropometric, hematological, and biochemical parameters in PCOS patients with that of normal females including correlation between interleukin-3 and blood cell count. Patients with PCOS and normal healthy females (n=60) of same age group were included in this study following Rotterdam ESHRE/ASRM...
S.Y.M. Mahmoud1 and M. Hashem2
...oating with viruliferous cystosori. the thick-walled resting spores of P. betae. as well as by root vortexing in virus inoculum containing carborundum. The virus is not adsorbed to the exterior of the fungal vector but is internalized. Our results indicated that BNYVV is a widespread in sugar beet in Kafr EI-Sheikh Governorate. thus, precaution methods must be taken for reducing virus transmitting to other free-virus regions. ...
Fadime Eroglu1*, Altan Akineden2, Cemal Cicek3, Selcuk Turkel2
... protozoan such as Blastocystis spp., C. parvum, E. histolytica and G. duodenalis. While intestinal protozoans were detected 22.3% (33/148) of COVID-19 patients, intestinal protozoans were not detected 77.7% (115/148) in this study. Blastocystis spp. was frequently detected 39.4% (13/33) followed by C. parvum 27.3% (9/33) then G. duodenalis 21.2% (7/33) and E. histolytica 12.1% (4/33) among COVID-19 patients. While all of th...
Kadhim Kh. K. Al-Khayat1*, Athmar K. A. Al-Azawi2
...nally to looking for any cyst may be founded grossly in the peritoneal cavity or on the visceral organs, all cysts were examined for their structures scolex, membrane and the size measurements. The fluid was collected from each cyst for measurement the volume, and some cysts were stained with a cetoacetic acid carmine stain to seen the structure of
Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3
...ls (2.94 %). Perimetrial cysts in 9 animals (2.65%). Furthermore, many bacteria were isolated and the most common was Klebsiella oxytoca, Proteus vulgaris, Proteus vulgaris and Enterobacter aerogenes. The conclusion is that uterine disorders play a crucial role in female buffaloes’ infertility. It is highly recommended to use specific clinical parameters and biopsy techniques for early diagnosis in order to proceed with the appropriate treatment.
...
ASMA WAHEED QURESHI1* & SHUMAILA ALAM2
...ction of parasitic eggs, cysts and larvae. Seven vegetables including cabbage (Brassica oleracea), lettuce (Lactuca sativa), carrot (Daucus carota), mint (Mentha spicata), chili (Capsicum frutescens), cucumber (Cucumis sativus) and corriander (Coriandrum sativum) were assessed using standard methods. Overall parasitic contamination of 30.7% was observed. Highest contamination was detected in Mardan (36%) followed by Katlang (30%) while the lowest contamination...
Habibeh Jabbari
...align: justify;">Cabbage cyst nematode, Heterodera cruciferae Franklin, 1945 causing serious damage on different cabbage cultivars and other host plants. High incidence of this nematode revealed by soil and root samples in the vegetable growing farmlands in Tabriz, Iran. Different populations of H. cruciferae were collected from the region and characterized based on morphological, morphometric and molecular features, four out of those populations isolated from...
Shangmei Peng1 and Changhe Yu2*
...T group decreased, while cystatin C and eGFR increased after the examination, with statistical significance (P<0.05). But there was no significant difference in creatinine, urea, urea nitrogen, cystatin C and eGFR between the two groups at the same time point (P>0.05). The incidence of eGFR 5 patients in the two groups was higher than that in other stages, and the difference was statistically significant (P<0.05). E...
Tabassum yaseen1*, Muzamil shah1, Khushnood Ur Rehman2 Ali Mujtaba Shah3, Gul
Nawaz1,Rani Gul4
...by Aculospora, and Sclerocystis. The lowest Glomus spore
density is present in Fumaria parviflora (10.67±15.89). The spore density of AMF had a
strong positive correlation with soil pH and a negative correlation with the Phosphors
content of the soil. The highest vesicles are investigated Euphorbia heliscopia (14.33±9.5)
and the lowest in Cirsium arvense (3.33±1.53). Arbuscules were high in E...
Kiran Rani1*, R. Aliya1, Barkat Ali Solangi2, M. Kashif Pervez2, Nasrullah Akhtar3 and Farman Ahmed4*
Iqra Haider Khan1, *Arshad Javaid1 and Nadeem Shad1
...es
sterigmatocystin, a carcinogenic and hepatotoxic mycotoxin. This study reports the
usefulness of polar and non-polar fractions of methanolic extract of hemp (Cannabis sativa
L.) leaves against A. versicolor. Dried leaves of hemp were soaked in methanol for two
weeks. The material was filtered and the methanol was evaporated on a rotary evaporator.
Thereafter, water was added to the residues and the...
Sri Purwanti1*, Wempie Pakiding1, Marhamah Nadir1, Nurhayu2, Kusumandari Indah Prahesti1, Sitti Nurani Sirajuddin1, Jasmal Ahmari Syamsu1,4, Andi Mushawwir3
...id-binding protein), homocysteine, and Gamma-glutamyl transpeptidase found higher (p<0.05) in R0 than R1 and R2. Moreover, the level of adiponectin, apolipoproteins, HDL (high-density lipoprotein) cholesterol, LDL (low density lipoprotein) cholesterol, triglycerides and NEFA (non-esterified fatty acid) was found significantlyr (p<0.05) better in R0 compared to the R1 and R2 groups. These results suggested that inclusion of maggot, indigofera and turmeric...
Farhatul-Ain Arshad1, Asia Bibi1*, Amna Mushtaq1, Rubaida Mehmood2 and Nahid Kausar1
...align: justify;">The polycystic ovarian syndrome (PCOS) is an endocrine disease in reproductively aged women and is charactered bydyslipidemia, hyper-androgenism, acne, hirsutism, menstrual irregularities, and ovarian dysfunction resulting in infertility. A balanced body mass index, physical fitness, biochemical and endocrine ratios play vital role in proper functioning of reproductive system and guard against PCOS development. This study was conducted to inve...
Mohammed Mijbas Mohammed Alomari1, Nawar Jasim Alsalih2*, Samir Sabaa Raheem2, Mohenned Alsaadawi2, Ali Mosa Rashid Al-Yasari3
...Puppies with Diarrhea, oocysts of Cystoisospora canis were identified. The suspected cases were diagnosed by using a rapid test for parvovirus. The effectiveness of the method of therapy for dogs with parvovirus enteritis complicated by associations of coccidia in a severe form of the course of the disease was evaluated in two stages. The first stage was carried out at the time of the appointment of treatment, and the second...
Hind A.Norii1, Adel M.Hassen ALZobidy2*
...f female rats....
Roy Hendroko Setyobudi1, Shazma Anwar2, Mohammed Ali Wedyan3, Damat Damat1*,
Yogo Adhi Nugroho4, Tony Liwang4, Praptiningsih Gamawati Adinurani5, Satriyo Krido Wahono6,
Evika Sandi Savitri7, Bayu Agung Prahardika7, Irma Rahmaita Utarid8, Iswahyudi Iswahyudi9 and
Hemalia Agustin Rachmawati1
...hreonine, isoleucine and cysteine were noted higher in CCF from La Boitê (0.80±0.01), (1.03 ± 0.01), (1.05 ± 0.02), (0.72 ± 0.00) and (0.61 ± 0.04) while lower in Mengani Farm (0.16 ± 0.00), (0.02 ± 0.01), (0.27 ± 0.15) and (0.43± 0.08) respectively. Glutamic acid and proline were found more in Karang Ploso Farm (1.83 ± 0.01) and (0.92 ± 0.00) while less in Mengani Farm (1.09 &p...
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
...loes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro maturation (IVM) conditions for buffalo blastocysts. The tissue culture media included a control culture medium and other three LC supplemented media (2, 3 or, 4 mM LC). All LC treatments had higher C...
Hasan Ali Al-Sailawi1, Araa Ali Hadi1, Hussein A. Raheem2, Mustafa Mudhafar3,4, Sahi J Dhahi5, Qais R. Lahhob6*
...male rabbits by N-acetyl cysteine (NAC) and Serratiopeptidase and then compare their effect on the skin grafting of rabbits. Nine rabbits were divided into three groups. Each group had three animals: Group 1 (G1) was used as a control (skin grafting without any drug), and the skin was taken from the shoulder to the femoral area. In group 2 (G2), the skin graft was taken from the same area in the G1 using Serratiopeptidase, while in group 3 (G3), the skin graft...
Samah Abou Asa1, Omnia Tag2 and Walid Elmonir2*
...l techniques to detect encysted metacercariae (EMC) of Heterophyids in mullet (n= 50) and tilapia (n= 50) fish and describe their pathological effect on fish meat quality. Also, knowledge, attitude and practices (KAPs) related to fish-borne parasitic zoonoses among 100 residents in Kafrelsheikh Governorate were also investigated. The overall prevalence of EMC infection in examined fish was 32% (32/100): 44% (22/50) in mullet and 20% (10/50) in tilapia fish sam...
Mustafa Abd El Raouf*, Mostafa Abdelazim, Abdel Basit M. Abd El-Aal, Shebl E. Salem
...ically treated with tube cystostomy technique and divided into two groups; Group I (n=10): orally administered ammonium chloride 200mg/kg and Group II (n=10): Local introducing of Walpole’s solution 100mL/animal. The time of resuming normal urination and urine pH intraoperatively and after recovery was measured. In Group I, 30%, 50% and 20% of animals resumed their normal urination within 3, 7 and 14 days postoperatively respectively meanwhile in Group I...
Walid Elmonir1*, Ahmed Abdel-Fattah Tayel2, Suzan Abdelbaky Kotb1 and Wael Fawzy El-Tras2
...samples had Toxoplasma oocysts. T. gondii was detected in 14% of carrots, 12% of radishes, 4% of lettuces, and 6% of strawberries. There was no significant difference in prevalence of T. gondii between different fresh produce type (P value= 0.1 – 0.6). T. gondii was not detected in any fish samples. Two agriculture produce (1 carrot and 1 strawberry) samples were misdiagnosed by microscopic analysis, which emphasized the importance of molecular detection...
Kamal Ahmed El-Shazly1, Amira Abd El-Latif1, Nagwa Elhawary2, Asmaa F. Alswaf1, Foad Farrag3, Samy Sayed4, Mohamed El-Sharnouby5 and Mustafa Shukry6*
...water. On the 15th the oocysts of the Eimeria tenella parasite were implanted orally into all but the negative control groups. According to the current findings, G2 demonstrated a significant increase in feed conversion rate, oocysts count, lesion score, mortality rate, count total leucocytic, ALP, ALT, AST, total bilirubin, creatinine, urea, uric acid, and MDA level, the body weight significally reduction, weight gain in th...
Duaa Jasim Mohammad
...parenchymal degradation, cystic dilatation filled with blood, granuloma, lipid accumulations, and inflammatory cell aggregation. After exposure to a 2% and 3% concentration of halothane, tissue sections of the liver revealed extensive aggregation of Kupffer’s cells, granuloma, lipidosis, bile buildup, and extremely broad sinusoidal dilatation in different parts of the liver parenchyma, as well as a high number of inflammatory cells present around the aff...
Muhammad Noman Tariq1, Saeed Ahmed*1, Ghazanfar Ali Chishti1, Muhammad Shahbaz Zafar1, Muhammad Saadullah2, Muhammad Usman1, Mohsin Ali1 and Ghulam Qadir1
Mohammed Jawad Kadhim*, Qasim Jawad Amer
...he existence of parasite cysts and trophozoites in both human and cattle fecal samples. The PCR results indicated that the genus Entamoeba was detected in 43% (43/100) of the human fecal samples (27 in male and 16 in female). Moreover, the results showed that the high frequency of E. histolytica was in children less than 5 years old (48.6%) as compared to patient from 5-10 years and patient more than 10 years where a rate of 38.2% and 41.4% was observed, respe...
