Shakeel Ahmed* and Sarwat Jahan
...Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant (P>0.05) change in adiponectin levels was observed after NMS injection in both normal and metabolically stressed conditions. NMS administration induced a significant (P<0.01) increase in resistin levels, while suppressed leptin (P<...
Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*
...nked immunosorbent assay kits and an indirect hemagglutination test. The results showed that seroprevalence in Tibetan pigs were 25.6% by ELISA. On gender basis, the prevalence was found 25.1% in males and 27.8% in females. By the method of IHA, the seroprevalence in Tibetan pigs were 21.6%, and the prevalence was found 23.1% and 21.4% in males and females, respectively. The results indicates that the Tibetan gigs were exposed to T. gondii in Tibet area, which...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...ts also indicated that c-kit expression was higher in the white cashmere goat than in the black goat, and this significant difference was detected by q-PCR and western blotting. All cashmere goats of different colors examined by immunohistochemical analysis showed either weak (the black cashmere goat) or strong (the white cashmere goat) expression of the KIT protein. These findings ...
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...ting purpose while black kite Milvus migrans utilized semal tree Bombax ceiba. The weaver bird Ploceus philippinus also utilized only Acacia nilotica for nesting. The common crow, common myna, black kite, black drongo, red turtle dove Streptopelia tranquebarica and Indian robin Saxicoloides fulicatus successfully bred in particular nests whereas red wattled lapwing Vanellus vanell...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...ence of EIV. Using ELISA kit and HI as serological methods for diagnosis, we found a 24.13% (168/696) overall seroprevalence, where the seroprevalence for H3N8 was 14.51% (101/696), for H7N7 6.03% (42/696), and 3.59% (25/696) as mixed infections, suggesting EIV current and active circulation in equine population of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in pon...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...racted using R Neasy Minikit (Qiagen) according to instruction manual. After extraction RNAs were eluted in 60 ul elution buffer and stored at -70C. Each strain of FMD virus was serially diluted to -5dilution in order to check the efficacy of test. Reaction was completed in 1 hr and 10 min at temperatures 600C. It was also tested in hot water bath on same temperature. Results were same in both techniques. Results showed that LAMP was more sensitive ...
Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain
...g commercially available kit i.e. protinase-K (sigma). 27 out of 34 isolates were confirmed as Cmm having 614bp band. Black nightshade (Solanum nigrum L) was the only weed from which pathogen was consistently isolated. From Northern KP, a total of nine weeds including; Solanum nigrum L., Solanum americanum Mill., Solanum Sarrachoides Sedntner, Amaranthus blitoides S. Wats, Amaranthus albus L., Lactuca serriola L., Amaranthus retroflexus L., Malva parviflora L....
Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1
...LISA using Human pro BNP kit. Patients’ history and recorded blood pressure was documented on a questionnaire. The results were analyzed using SPSS 21. One way ANOVA and Independent sample T test were applied to compare the proBNP levels. A P-value of < 0.05 was taken as significant. Serum pro BNP levels were raised in migraine patients and also in hypertensive patients with migraine (p-value 0.00). Serum pro BNP levels were found to be raised in both...
Hamayun Khan1,*, Abdul Wahab1, Navaid Kazi2, Younas Muhammad1,Summaya Kazi3, Salman Kazi4, Azmatullah Khan1, Ikramullah Khan5 and Shakoor Ahmad1
... commercially accessible kits for biochemical markers that includes glucose, total protein, albumin, creatinine, triglycerides, blood urea nitrogen (BUN), alanine transaminase (ALT) and aspartate transaminase (AST). The current study demonstrated the ascertained value in amniotic fluid for glucose, total protein and albumin at day 18, 25 and 28 was 42.9±0.80, 37.4±0.90, 36.5±0.88mg/dl; 17.9±0.30, 16.1±0.22, 13.9±0.34g/...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
...unosorbent assay (ELISA) kit to test the prevalence of BVDV(Bovine Viral Diarrhea Virus) in yaks in Hongyuan, Sichuan province, a cold climate and high altitude area of China. Totally, 516 yak serum samples were randomly got during 2012 to 2014. BVDV was found highly prevalent among the adult yaks (51.1%; 95% CI: 46.5-55.6) and calves (60.5%; 95% CI: 43.4-76.0), respectively. The prevalence of BVDV infection in 2012 to 2014 was 45.3% (95% CI: 38.6%-52.1%); 56....
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
... 
Sana Rafique1, Hina Saqib1, Khushi Muhammad2, Nazia Akbar2, Abdul Rauf3, Muzafar Shah4,*
...n using Rapid Diagnostic kit for Dengue virus (DENV) i.e. SD BIOLINE Dengue IgG/IgM. Data was obtained from patient’s record, filled forms and through questionnaire. Total 1332 blood samples were collected from 3 tehsils of District Mansehra. Out of which, 725 were found positive for dengue fever infection. Out of 725 positive cases, 410 (56.5%) were males and 315 (43%) were female’s patients. The high rate of DENV infection was found in the...
Qudsia Yousafi1,*, Muhammad Afzal2, Muhammad Aslam3 and Allah Ditta Abid4
...uring 2013 in Sahiwal, Pakitan using brinjal variety, Nirala. The experiment was laid out in Randomized Complete Block Design with four treatments each repeated three times. The treatments were T1= removal of infested shoots, T2= Use of light trap, T3= T1+T2 and T4=control. The seedlings were transplanted on April 21, 2013 keeping plant to plant distance of 30cm. Kerosene oil lantern, hung over metallic tray filled with water, was used as light trap. Data for ...
Ocheme Julius Okojokwu1*, Maryam Bisola Adebayo2, Bashiru Shafa Abubakar3, Ibrahim Abubakar Yusuf2 and Joseph Aje Anejo-Okopi1
...c Parvovirus B19 IgG/IgM kits. A total of 171 (52.5%) of women had IgG antibody to Parvovirus B19 while 31 (9.5%) were positive for IgM antibody. Demographics including age group, educational status, occupation, type of marriage, number of children, history of blood transfusion and trimester of pregnancy were statistically associated with prevalence of IgG antibody (p ≤ 0.05). There was a significant association (χ2 = 12.571; p = 0.028) between age grou...
Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq2
...cial latex agglutination kit (Antec Diagnostic products, UK). The overall prevalence was 26.76%. The significant (P<0.05) higher seroprevalence was observed in males (34.28%) than in females (19.44%). For analysis of hematological parameters blood samples were collected. The mean values for hemoglobin (Hb), Packed Cell Volume (PCV), Red Blood Cells Count (RBC), Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Con...
Saima Naz*, Adil Ali Rajpar, Abdul Hameed Chandio
New Records of some Phthiraptera (chewing lice) of birds from urban areas of Hyderabad, Sindh, Pakistan
... 1866) from black
kite. All were new records from Hyderabad. Bank myna was recorded as new host for M. eurysternus and black kite
was reported first time harbouring Degeeriella regalis from Pakistan....
kite. All were new records from Hyderabad. Bank myna was recorded as new host for M. eurysternus and black kite
was reported first time harbouring Degeeriella regalis from Pakistan....
Nabila Roohi, Mehjabeen, Samina Ashraf*
Effects of cigarette smoking on serum proteins profile in male active and passive smokers
...rcially
available kits by using automated clinical chemistry analyzer. Serum total proteins
have shown a significant decrease in smokers in comparison with controls. A
significant depression in serum albumin was observed in smokers due to increased
excretion of albumin in bound state with the chemicals, whereas, total globulins,
gamma globulins and non-gamma globulin serum proteins have shown significant
increase in sm...
available kits by using automated clinical chemistry analyzer. Serum total proteins
have shown a significant decrease in smokers in comparison with controls. A
significant depression in serum albumin was observed in smokers due to increased
excretion of albumin in bound state with the chemicals, whereas, total globulins,
gamma globulins and non-gamma globulin serum proteins have shown significant
increase in sm...
Haniya Mazhar1, Naaz Abbas2, Tehseen Zamir3, Zahid Hussain1, Syed Shahid Ali4*
...le oil filling areas and kitchen wastages were screened on tributyrin agar and rhodamine agar plates. The strain PCSIR NL-37 was identified as Bacillus cereus following morphological, biochemical and molecular characterization. Best medium for lipase production was olive oil and mustard oil cake and the best carbon sources were fructose and maltose whereas yeast extract was found to be the best nitrogen source, showing 55U/mL enzyme activity. The best i...
Muhammad Amir Iqbal1, Nabila Roohi1*, Ahmad Qureshy2
...lly available diagnostic kits were used to determine the serum levels of FT4, FT3, TSH, Creatinine, AST and ALT. Prominent reduction (P<0.001) of creatinine was observed in overt hyperthyroid state, when compared with controls. However, significant (P<0.05) improvement was noticed in it in post treatment group. Pronounced elevation (P<0.001) of ALT was observed in overt state when compared with controls. Subc...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
... API® 20E kit. Antibiogram was determined by disc diffusion method and isolates were screened for ESBL production using double-disc synergy test (DDST). Molecular detection for the presence of bla-SHV and bla-TEM was carried out by polymerase chain reaction (PCR). Of 392 blood and urine samples, 120 were found positive for microbial growth. Antibiotic susceptibility testing of K. pneumoniae isolates illustrated high resistan...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
Zafar Abbas1*, Muhammad Mubashir1, Umair Riaz1, Zeenat Javid1, Muhammad Ashraf1, Saeed ur Rehman1, Muhammad Javid Qamar1, Syed Ali Zulqadar1 and Shahzada Munawar Mehdi2
...form of fertilizers like kitchen waste, poultry manure and compost were used while the inorganic sources were NPK fertilizer. This experiment was laid out in the field area of soil and water testing laboratory for research, Bahawalpur in growing seasons from July to October of 2017. Treatments were arranged in Two factorial Completely Randomized Design CRBD fashion with three replications and six treatments. Treatments are arranged as control T1 (Without ferti...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...ercially available ELISA kit for the detection of antibodies against Mycobacterium avium subsp. paratuberculosis (MAP). Data were analyzed using chi square for ELISA and odds ratio for finding association of species, gender, body weight, age and body condition score (BCS) with prevalence of antibodies against MAP. Seropositivity against MAP was significantly (P<0.05) detected. Male and female animals were equally susceptible to the disease (OR...
Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3
...munochromatographic test kit. Subsequently, they were passaged in HEp-2 cell line. All isolates were subjected to RNA extraction, cDNA synthesis, three (5l-UTR, VP1 and EV Species C [EV-C]) different PCR assays and sequencing of amplicons. EVs were further subjected to [‘]Illumina sequencing. All CCS were negative for EAVs and GARVs. Four CCS produced R-CPE in HEp-2 cell line, three of which were positive for the 5l-UTR assay. Of the three isolates, two ...
Sameera Arshad1*, Arshad Mahmood Malik2 and Iftikhar Hussain3
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*
...tate dehydrogenase assay kit; superoxide dismutase (SOD) activity and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via the methylation-specific PCR (M...
Muhammad Nisar* and Muhammad Idress
...me gardening also called kitchen or homestead gardening. Home gardening study is necessary not only due to its social, food security and economic contribution, but its consideration’s negligence in development agendas. Home gardening data provide complete agricultural input in a nation’s domestic treasure. Therefore, the current study was carried out with the objective to assess small farmers’ involvement in home gardening at district Tank, K...
NaseemZahra1,2,*, Nadia Jamil1, Sajid Rashid Ahmad1, Imran Kalim2, MuhammadKhalid Saeed2, Ijaz Ahmed2, Qurat-ul-ain Syed2 and Soniya Munir1
... using competitive ELISA kit method. Branded samples (n=40) were collected from different shops in Lahore city of Punjab Province while non-branded samples (n=40) collected from four selected towns of Lahore. Overall 58% and 100% of aflatoxin contamination in branded and non-branded milk samples were found, respectively. The quantities of AFM1 in 58% of branded milk samples and 95% of non-branded milk samples were found beyond permissible limits of 50 ppt as s...
Olufisayo Bamidele* and Safiriyu Idowu Ola
...ls, kindling rate, total kit born, total born alive, still born, weight of kits at birth and gestation period (days) and were analyzed using Analysis of variance of the General Linear Model procedure and t-test where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. Kindling rates were 10%, 10%, 60% and 50% respectively for the groups. There was no significant differenc...
Bamigboye Olutoyin Omolara1, Fapohunda Funmilayo Omotoyosi1, Oladipo Elijah Kolawole1,2, Oni Mattthew Olujenyo1, Ajibade Oluwatosin Akinola1, Kaka Mary Oluwatosin1*
...a third generation ELISA kit for screening. The mean CD4+ count and age were 479.12 ± 23.48 cells/µl and 48.56 ± 1.27 years, respectively. Only six samples out of ninety-three HIV-positive samples were positive to HBsAg while fifty-four samples (58.1%) were positive for HBcAb however, HBeAg marker was negative among all samples. Interestingly, the prevalence of the HBV infection was more in females (66.6%) compared to the male counterparts ...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*
... a marketable ELISA test kit for assessment of Pregnancy Associated Glycoproteins (PAGs) in peripheral blood for early pregnancy identification in cattle, buffalo, goats and sheep. A total of 120 blood were taken from jugular vein of different breeds of cattle (Achai, Achai x Jersey, Holstein Friesian and Jersey), buffalo (Nilli Ravi, Aza Kheli and non-descript), goats (Beetal, Teddy and non-descript) and sheep (Bulkhi, Karri and non-descript). In cattle, the ...
Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5
...CV screening through ICT kit method. All ICT positive samples were further confirmed through ELISA. Individuals 25(8.2%) were found positive for both hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. All the ICT positive individuals were further conf...
Yousaf Ali1, Muhammad Zamin1*, Ibadullah Jan1, Shahen Shah2, Muhammad Mazhar Hussain3, Fazli Rabbi4 and Muhammad Amin5
...s (Nadir, Money Maker, Pakit, Nagina, Roma and Rio grande) with three replications. The results revealed that the combination of compost and mixture has significant effect on shoot height, number of leaves, stem and root length, however stem diameter was significantly affected by compost and non significantly affected by genotypes. As far as the genotypes performance is concerned, genotype money maker showed the best performance for all the agronomic character...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
Syed Basit Rasheed*, Ahmad Yar, Farrah Zaidi and Qaisar Jamal
...omly from indoor (rooms, kitchen and washrooms) and outdoor (streets, crop fields, barren ground and grave yards) sites by using buccal aspirator, pitfall traps and hand pick method. A total of 19 species representing 11 genera and three subfamilies namely Formicinae, Myrmicinae and Dolichoderinae were identified during the present study. Myrmicinae was the most diverse subfamily with five genera (Crematogaster, Meranoplus, Messor, Monomorium and Phe...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...using a commercial ELISA kit. Twenty seven (5.4%) and thirty five (7%) samples were IgM and IgG positive, respectively. All antibody positive samples and a subset of negative samples (195) were further subjected to PCR for confirmatory purposes. Two different sets of primers were used for the identification of the viral genome. Thirty samples were found PCR positive and a subset of ten samples was direct sequenced. Sequence analysis showed 94% similarity to
Cornelius Arome Omatola*, Samson Daniel Iyeh, Shedrach James Abuh, Charles Kehinde Mofolorunsho, Martin-Luther Oseni Okolo and Phoebe Queen Akoh
...unochromatographic based kits and for syphilis using non-treponemal and treponemal antibody test. Socio-demographic and obstetric parameters were obtained using structured questionnaires. Sixty-four (25.6%) out of 250 women had at least one STI. The overall prevalence of syphilis, HIV, HBV, HCV and HSV-2 were 28(11.2%), 21(8.4%), 10(4.0%), 5(2.0%) and 0% respectively. Women who were married, unemployed, aged 15-35 years and of low-income earners had a higher p...
Behram Khan1, Amir Nawaz Khan2, Said Qasim3, Atta-ur Rahman4
ANALYSIS OF HUMAN PERCEPTIONS AND ADAPTATIONS TO FLASH FLOODS IN KHYBER AGENCY, FATA
...lood ties with the land, kith and kinship. In the study area, people had adopted various flood mitigation strategies to reduce the devastating impacts of flash floods. However, the resident had greater motivations and concern on socio-economic factors rather than adjustments to frequent flash flood....
Nadeem Abbas Shah1*, Ejaz Ashraf1, Hafiz Khurram Shurjeel2, Zaheer ud Din Mirani3, Usman Rafique1 and Raees Abbas Shah4
Assessing Perceptions of the Households-heads Regarding Food Security Status in Drought-hit Areas of District Tharparkar, Sindh, Pakistan: A Case Study for Agricultural Extension
...ls’ food products, kitchen gardening, reuse of preserved food and other products, migration to food secure areas and reduced number of meals eaten in a day. It is recommended that government should focus on education, employment opportunities, development of natural resources for producing food secure crops and start awareness movements among the inhabitants of Tharparkar to revitalize the status of food security in the study area....
Joseph Anejo-Okopi1*, Dorcas Yilger Gotom1, Noble Allison Chiehiura1, Julius Ocheme Okojokwu1, David Ochola Amanyi2, John Otumala Egbere1, Joshua Adetunji1, Otobo Innocent Ujah3 and Onyemocho Audu4
The Seroprevalence of Zika Virus Infection among HIV Positive and HIV Negative Pregnant Women in Jos, Nigeria
...unosorbent assay (ELISA) kit to qualitatively analyze Human Zika virus IgG (ZV-IgG) in human serum. A total of 90 pregnant women was recruited, and the overall seroprevalence was (14.4%) for Zika virus (20.0% HIV positive and 8.9% negative) IgG antibodies. A significant association was found with age category among the HIV negative pregnant women p=0.012, and in the history of blood transfusion among the HIV positive participants, p=0.013. No association with ...
Shakeel Ahmed* and Sarwat Jahan
...Enzyme Immunoassay (EIA) kits. 48 h fasting significantly (P<0.001) decreased plasma T levels but it did not cause any significant (P>0.05) change in plasma GH levels compared to normal fed monkeys. NMS injection induced a significant increase (P<0.05) in T and GH concentrations compared to saline treated animals suggesting the possible involvement of GH in NMS induced secretion of testosterone. In summary our results suggest that NMS is a positive mo...
Yihong Tian, Yongmei Qi*, Sajid Naeem, Ke Gao and Yingmei Zhang*
...rmined using a LDH assay kit. Mitochondria membrane potential (MMP) was measured by flow cytometry. LC3 conversion and p62 expression were evaluated by western blot analysis. MTT assay revealed that spermidine decreased the viability of Hela cells in a dose-dependent manner in conjunction with disrupted morphology. Moreover, spermidine caused cell damage as evidenced by elevated LDH leakage and compromised MMP as demonstrated by flow cytometry. Concomitantly, ...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
... Cheaper eDNA extraction kit was a potential aspect for improvement to expand further application of eDNA analysis. In order to examine some cheaper kits’ effectiveness of extracting eDNA of macro-organisms and select an optional low-cost kit, a test among three kits was held. 12S rRNA bands of marine fishes on the agarose gel preliminarily reveale...
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
...e assayed using an ELISA kit. Pregnancy detection was performed by US on 23, 35, and 60 days post NM. Serum P4 concentration was significantly higher in pregnant than non-pregnant goats at 15, 23, 35, and 60 days post NM. Relative expression of mRNA of ISG15 and ISG17 was significantly higher in pregnant goats at 7, 15, and 35 days post NM. ISG15 and ISG17 were not significantly different compared to P4 and US in the pregnant and no...
Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6
Nilgun Dogan1,* and Hakan Adanacioglu2
... a survey was done in Kelkit County, in northeastern Turkey. The performances of the beekeeping farms were evaluated by cost, profitability and efficiency analysis. The cost of a unit of honey was US$3.16 and the average price paid to the beekeepers was US$6.78. The absolute and relative profits were calculated as US$3.62 and US$2.14, respectively. The efficiency of the beekeeping farms was measured using Data Envelopment Analysis. The results of output- and i...
Samina Tahir Kiani1, Abdul Rauf1, Syed Ayaz Kazmi1,*, Nuzhat Shafi1, Madiha Khalid2, Naeem Latif1, Samina Gul2, Saghir Yousaf3 and Muhammad Arshad4
...MTB antibodies using ICT kits. Sputum microscopy for MTB was done in MTB antibody positive samples. The samples positive in initial screening were confirmed by enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Nine samples were found positive in both ICT and ELISA test for HCV while PCR showed six positive cases for HCV. HBV were found positive in three samples after ICT and ELISA, but was found negative after PCR. MTB was found po...
Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.
...eroxide dis-mutase assay kit. Genotyping was performed by polymerase chain reaction and re-striction fragment length polymorphism (PCR-RFLP) method. The homozygous Ala/Ala (CC) was significantly more frequent in HCV-related HCC patients com-pared to HCV-infected patients and control group (67.5%, 12%, and 15% respective-ly, p ˂ 0.05). Moreover, the C allele was more often associated with HCV-related HCC patients compared to HCV-infected patients and control g...
Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3
...natural infection. ELISA kit used for FMDV antigen detection for the epithelial suspension from 42 field samples collected from various geographic locations 10 governorates are ( El-Sharkia,Giza, Port Said, Assuit, Suize, Kafre El-Shake, Qina, Al-Gahrbia, Domiatte and Alexandria) Serotyping and molecular characterization by polymerase chain reaction PCR for (31) samples as FMD Serotype O , (2) Serotype A and (3) serotype SAT2 the samples collected from outbrea...
Serag eldeen Sultana, M.W Abdel azeemb, Nahla Mohamed Eldamranyc
...screen AI Antibody ELISA kit coated with AIV nucleoprotein and hemagglutination inhibition (HI) test against H5 and H9 antigens. The overall results using both ELISA and HI tests revealed that the prevalence of AIV antibodies was 12.6% (18/143). The seropositive samples represented 5/143 and 15/108 for ELISA and HI, respectively. The detection of both H5 and H9 antibodies in (4) samples indicated that co-infection may occur, while (10) samples were only contai...
Coronavirus Disease (COVID-19) with Special Reference to Pakistan: A Review on its Different Aspects
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...nment has to get testing kits and primers from other countries. Only few quarantine centers are available and there is shortage of drugs, beds, trained doctors and paramedical staff. Unfortunately, no vaccine is available yet so the only management strategy is the prevention of infection by wearing masks and following social distancing. Cleanliness, hygiene and quarantine measures are the key to stop any epidemic to pandemic and is known to human being through...
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1,
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
...al rapid in-clinic ELISA kits. Ticks were collected from dogs and molecular detection methods were used to identify the tick species and CME by amplification of the cytochrome oxidase subunit I and disulfide oxidoreductase gene, respectively. The results indicated that 1.29% of the serum samples were positive for E. canis, and 5.50% of dogs were infested with ticks. The Wenzhou samples of R. sanguineus exhibited a high homology (99.7%–99.8%...
Asim Faraz1*, Abdul Waheed1, Nasir Ali Tauqir2 and Muhammad Shahid Nabeel3
...tudied by using standard kits through biochemistry analyzer. The glucose, cholesterol and triglyceride values (P<0.05) were found to be 136.68±5.46, 57.12±4.44, 35.18±3.26 and 142.62±4.38, 59.32±4.26, 36.97±3.63 mg/dl respectively in August and February, being elevated in rut-camels. The total protein, urea and creatinine concentrations varied non-significantly among animals. While the calcium and phosphorus concent...
Amjed Ali1, Muhammad Tayyab1,*, Sehrish Firyal1, Abu Saeed Hashmi2, Asif Nadeem3, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...-lactamase from Hi-media kit available in the market. The β-lactamase from current study was found suitable for its use as positive control in antibiotic susceptibility testing and this enzyme will be utilized for the development of antibiotic susceptibility testing kit at domestic level in near future.
...
Kemi Funmilayo Omotesho1*, Philip Akintunde Fatodu1 and Toyin Benedict Ajibade2
...ior of youth farmers in Ekiti State. Specifically, it assessed the level of record keeping among farmers; examined their attitude towards record keeping; assessed the farmers’ knowledge of record keeping; determined their intentions towards keeping farm records; and identified the constraints to farm record keeping. A three-stage random sampling technique was used to select 178 youth farmers on whom a structured interview schedule was administered. Data ...
Abdullah Abdo Albegali1, Tayyaba Aftab1, Atiq-ur-Rehman1*, Amir Rashid1 and Mayada Mohammad1,2
...analyzed using different kits of Roche Cobas C111 chemistry analyzer. Histopathological investigation of liver and heart was also done. The plant significantly (P < 0.05) and non-significantly (P > 0.05) decreased the levels of total cholesterol, triglycerides, high density lipoprotein, low density lipoprotein, very low density lipoprotein, asparate aminotransferase and alkaline aminotransferase. The plant maintains the level of HDL to a normal range. It...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
... 0.50% AZO and control + kitasamycin (as AGPs). The results indicate that LBGW, ADG and FCR was significantly (P<0.05) improved in AZO 0.25% and 0.50% than the control. Eviscerated (EV), breast muscle (BM) and leg muscle (LM) were significantly (P<0.05) higher in the AZO 0.50% treated groups than control. Digestibility of DM, CP, AME, Ca and P digestibility significant (P<0.05) higher in AZO 0.25% and 0.50% dietary treatments. Moreover, jejunal amylas...
Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6
Bikash Puri1, Anil K. Tiwary2, Bharata Regmi3,4, Dinesh K. Singh1, Doj R. Khanal5 and Manoj K. Shah3*
...s in sera using a cELISA kit. Out of 220 sera samples, 92 were positive for BTV, accounting for 41.8% prevalence in ruminants. Seroprevalence rate was the highest in Buffaloes (58.3%) followed by sheep and goats (each 40%), and cattle (37.5%). The BTV seropositivity varied significantly (p<0.001) among ruminants in different sampling areas with the highest prevalence in Gokuleshwor (66.6%). Interestingly, the BTV was detected higher (p<0.01) in females (...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
... rapid tetracycline test kit. Second aim of the study was fined a safe way to control the hazards of tetracycline concentrations through study the influence of simmering and frying on these residues. Quantitative determination of tetracycline residues was done using High Performance Liquid Chromatography. The results revealed that the incidence of tetracycline residues was 260 (87%) in all examined chicken meat. The average concentration values of oxytetracycl...
Mudassir Safdar, Aurang Zeb, Sidra Khalid, Shahid Bashir, Riffat Mehboob* and Muhammad Imran
...r was provided to hostel kitchen for the study subjects. Blood hemoglobin concentrations and anthropometric measurements of all participating hostel girls (n=219) were determined initially (Day-0) and at the end of 4 months feeding period. Mean age of the subjects was 21.88 years, with an average initial BMI of 24.71 kg/m2. Findings of the efficacy trial showed that the average hemoglobin level in anemic girls increased from 8.99 to 9.94g/dl. The prevalence of...
Amira M. Refaie1*, Manal S. Mohamed1, Enayat H. Abo El-Azayem1, Sherein H. Mohamed2
...ant period and nursering kits till weaning. The results indicate that doe rabbits fed diets supplied with 0.15% AA recorded significantly higher bunnies’ size and total weight at weaning compared to control. Both groups fed either 0.10% or 0.15% AA recorded significantly higher total milk yield (7.71%) during the whole lactation period (5 weeks) comparing to un-supplemented group. All tested groups recorded a gradual decrease in Malondialdehyde (MDA) lev...
Zunaira Noreen* and Khawar Sultan
... abundant species. Black kite, house crow, and common myna were found to be making nests on trees only. A clear pattern of habitat selection is present in the study area where poplar is a winner species in richness of birds, but dominant eucalyptus Eucalyptus camaldulensis could retain only half the number of species than poplar. It is recommended that if native trees are required to be replaced by exotic trees Eucalyptus should not be the first choice, but pr...
Asmaa S. Mohammed*, Ahmed F. Abou-Elnaga, Ahmed I. Ateya, Mohammed M. Fouda, Ragab A. Darwish, Usama A. Abou-Ismail
...reased mortality rate of kits. Furthermore, the exposure to 8 hours of UVB radiations in particular improved reproductive indices including litter weight at weaning and Kits’ weight gain from birth to weaning, and up-regulated the relative expression levels of FSHR and ER-α genes. Therefore, based on the multiple measures approach used to evaluate the effect of UVB light on behaviour, reproductive and health status of rabbits, it could be suggested...
Abida Shehzadi*, Muhammad Shafique and Ahmad Ali Shahid
...ilerTM PCR amplification kit. Evaluation of statistical parameters for forensic importance revealed in recognition of 124 unique haplotypes with diversity value of 0.996. Locus DYS385a/b demonstrated highest power of discrimination, polymorphism information content and gene diversity as 0.907, 0.854 and 0.807 respectively. Analysis of Molecular Variance (AMOVA) and multidimensional scaling plot (MDS) were also generated by using online tools at YHRD database. ...
Mubashar Ahmad, Roheela Yasmeen*, Aisha Waheed Qurashi, Muhammad Hassan Ali Shah
...y radioimmunoassay (RIA) kit to determine the faecal and urine cortisol levels. Higher concentrations of cortisol were recorded in both males and females of blackbuck in the Jallo Park and Lahore Zoo as compared to Safari Zoo. Moreover, higher cortisol levels were recorded in the blackbuck in summer season as compared to winter season. Results of ANOVA showed significant differences in cortisol levels among different sites at <0.05 p-value. Temperature was ...
Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5
Judith Kiptoo1*, Daniel Mutisya2, Paul Ndegwa1, Ruth Amata3, Lucy Irungu4 and Rotich Godfrey5
Jiang Wu*
...reus cells. ATP analysis kit and EnSpire microplate reader were used to detect the intracellular ATP concentration of 4 foodborne pathogens. According to our results of all the tested samples, 38 (28.57%) were found to have Staph. aureus, 27 (20.30%) had Salmonella, 46 (34.59%) had E. coli, and 22 (16.54%) had L. monocytogenes. Staph. aureus had MIC of 0.55±0.18μg/ml and MBC of 1.13±0.11μg/ml; Salmonella had MIC of 0.25±0.13μg/ml ...
Feng Xu, Weijing Fan and Guobin Liu*
...easured using commercial kit, and expression levels ofNEXN-AS1 and miR-410-3p were detected using real-time quantitative PCR analysis. The dual luciferase experiment was applied to verify the targeted relationship betweenNEXN-AS1 and miR-410-3p. HUVECs were transfected with NEXN-AS1 overexpression vector, and then treated with ox-LDL for 24 h before detecting the activity, apoptosis rate and oxidative stress level of HUVECs. The low concentrations (25, 50, 100...
Asim Faraz1, Muhammad Yaqoob2, Nasir Ali Tauqir3, Hafiz Muhammad Ishaq1, Ayman Balla Mustafa4, Amir Ismail5, Muhammad Arslan Akbar6, Abdul Waheed1* and Muhammad Shahid Nabeel7
...ercially available ELISA kits were used to measure serum progesterone and estradiol levels. Progesterone and estradiol levels were found significantly high (P<0.05) with the value of 3.46±0.25 ng/ml at day 14th and 4.45±0.34 ng/ml at day 21st in G2 (Pregnant animals), while in non-pregnant group serum P4 concentration was recorded to be 1.05±0.29 ng/ml which is considered as basal level of progesterone in dromedary camel. Mean serum est...
Sara Mohamed Hemeda1, R.H. Sayed2, Hani Hassan1, Sheima A.E.2, Hassan Aboul-Ella3, R. Soliman3*
...atographic lateral flow kit (LFK) has been developed for the detection of β-lactams antibiotic residues in dairy milk. The developed LFK is based on a competitive immunochromatographic format using anti-antibiotic-specific polyclonal antibodies. Specific artificially induced active acquired polyclonal antibodies production were performed in female New Zealand rabbits against the following β-lactam antibiotics; Amoxicillin (AMX)- Keyhole Limpet Hemoc...
Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6
...plasma and milk by using kits of progesterone assay, and to confirm the diagnosis of COD, mostly trans rectal palpation and trans rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines....
Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*
...tion for preparing ELISA kits for clinical detection....
Shambel Boki Dabi
...y on site. A simple test kit was used to evaluate nitrite and ammonia levels. The result revealed a high level of alkalinity (66.3±39. 1mg. L-1) at downstream and lowest concentration (58±40mg.L-1) was found in the middle stream. The upper stream has a high CO2 concentration (14±3.6mg.L-1) while the middle stream has a low CO2 concentration (9.4±0.77mg.L-1). The maximum chloride content (28.7±2.6 mg. L-1) was found at downstr...
Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6
...flow cytometry and ELISA kits. Apoptotic and neuronal markers were evaluated using quantitative real time PCR and protein blots. Results showed that PCB inhibited the infiltration of inflammatory cells and improved the myelin protective proteins. It also positively regulated the antioxidants and apoptotic markers including Caspase-3, TGF-β, SIRT-1, CCL-2 and MBP. Moreover, PCB modulated the levels of inflammatory mediators and TGF-β in splenic cells....
Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1
...d using commercial ELISA kit. The results revealed that AFM1 was statistically higher (P˂0.05) in district Karachi (0.7223 µg/L) as compared to Thatta and Hyderabad (0.5897 µg/L and 0.5697 µg/L, respectively) than FDA limit (0.5 μg/L) however, it was found relatively similar (P>0.05) in Hyderabad and Thatta districts of Southern zone. In central zone, numerically AFM1 load was high (P>0.05) in samples of district Mirpurkhas (0.5306 ...
Gudeta Nepir Gurmu, Tade Bitima Mulisa, Alemu Lenco Gemechu, Kegna Gadisa Amena and Gemechu Nedi Terfa*
...ting of eight (Bursa, Burkitu, Adi, Herena, Hortu, Letu, T/shaman, and Weyib) improved field pea varietiesand one local variety at west Showa zone Oromia region to identify high yielding varieties. The experiment was carried out using a randomized complete block design with two replications at Babich, Goda Hora, Chelia Rafiso Alenga, EjersaLafo, and Goromti locations. Data on yield and yield-related traits such as harvest index, above-ground biomass, number of...
Naheed Zahra1*, Saira Batool1, Mubbashira Nazir1, Ghulam Akbar Malik2 and Iffat Batool3
... at the household level, kitchen gardening program should be introduced which will improve their livelihood more effectively....
Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2
...ercially available ELISA kit. The supernatants from oro-pharyngeal and cloacal swabs of the seropositive samples were separated and divided into two segments; one was used directly to detect AI viral genome through RT-PCR while other segment was used for viral inoculation into the embryonated chicken eggs. The direct detection through RT-PCR confirmed H9 gene from cloacal swab samples in 6.9% of the seropositive sample while we could not confirm any of the oro...
Jie Li, Gaofu Wang, Xiaoyan Sun, Lin Fu, Peng Zhou* and Hangxing Ren*
Mansha Tahir1, Abdul Rauf2,3, Syed Ayaz Kazmi2,3*, Nuzhat Shafi2, Muhammad Hasnain Ali Gillani3, Sundus Khawaja4, Syeda Maria Fiaz Bukhari4, Tehniat Summer4 and Muhammad Majid Bashir2
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
...p>TM amplification kit with 21 autosomal STR loci in our laboratory for validation purpose and routine use. In this regard, swab samples from different regions of Turkey (n=350) were collected and studied. The validation of the GlobalFilerTM Amplification Kit was carried out in accordance with the guidelines published by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Our study was conducted to show the efficacy and performance ...
Hongfeng Guo
...act were detected by the kit; the expressions ofprotein kinase B (Akt), Bax and Bcl-2 were detected by Western blot. According to our findings after drug treatment, the body weight and respiratory ventilation of mice returned to normal level compared with the intermittent hypoxia group. Compared with the blank control group, after intermittent hypoxia, the contents of MDA, SOD and PGE in the lower respiratory tract of SD mice were significantly lower than thos...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...d interpreted diagnostic kit as well as to be a competitive alternative to the conventionally established expert-dependent techniques. The current work’s main objective is to evaluate the reliability of two widely distributed rapid kits for as a suitable substitution for the gold standard bovine tuberculosis diagnostic techniques. IQRT Anigen Rapid Bovine TB Ab kit and Ubio quick VET...
Alsaied Alnaimy Habeeb*, Amaal M. Kamal, Mostafa Abbas A. Atta, Ahmed K. Sharaf
...y adding or removing the kits on the third day, the quantity of each litter for each mother was consistent with six, ensuring an equal number of litters at the beginning of the experiment. One of the two groups of animals received a random distribution of female rabbits. Ten does in group one acted as controls and remained with their kits in a group during free lactation (doe-litter together, DLT). In group two, ten does wer...
Safriyanto Dako1, Nibras Karnain Laya1, Syukri I Gubali1, Ari Ardiantoro2, V.M Ani Nurgiartiningsih3, Gatot Ciptadi3, Desinta Wulandari3, Suyadi Suyadi3*
Omnia M. Khattab1, Morcos I. Yanni2, Hala K. Abdelmegeed2, Mahmoud Eliwa1, Naglaa M. Hagag1, Sara M. Elnomrosy1
... using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the same animals were subjected for hematological examination to investigate the severity of leukopenia. Thirteen out of nineteen samples were positive with a polymerase chain reaction assay (PCR) targeting VP2 gene. Three positive fecal samples in animal showing sever and moderate leukopenia were s...
Yun Zhang*
...solated using commercial kit. The exosomes were observed by transmission electron microscope. Western blotting was done to detect the protein expression. Transmission electron microscope analysis revealed that exosomes could be significantly observed in the PE group. The expression of surface markers that included CD63 and CD81 were dramatically upregulated in PE group. The protein level of ET-1 and Ang-II were significantly increased in PE group, and the upre...
Tajul Islam Mamun, Jillur Rahman, Mahmudul Hasan, Md. Jamal Hossain, Md Wakil Mahmud and Kamruz Zaman
...xamination, FIP antibody kit test, blood test and Rivalta’s test. The prevalence of young cats was 6.94%, sexually intact cats 5.29% and 20% of cats in poor health conditions were significantly associated with the occurrence of FIP (p ≤ 0.05). Sex and breed were not statistically associated with FIP but had a comparatively high prevalence. Most of the suspected FIP clinical feature was effusive (66.66%). This outcome provides an empirical analysis of ...
Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1
..., and bacteria isolation kits were used for the isolation and identification of bacteria. The genus Vibrio, Aeromonas and Pseudomonas were identified in different components of the larval production line in the hatchery. V. mimicus, V. parahaemolyticus, V. harveyi and V. alginolyticus were the major species identified from the Vibrio genus. The average concentrations of Vibrio spp. in brood, brood’s tank water, eggs, larvae, larval tank water, and larval...
Ni Putu Sarini1*, I Gde Suranjaya1, I Gusti Agung Arta Putra1, I Wayan Suarna1, Lindawati Doloksaribu1, I Ketut Puja2, I Ketut Gde Natakesuma3, I Gusti Ngurah Bagus Rai Mulyawan4
...acted using a commercial kit and the MC1R gene was then amplified by using a forward primer 5’ AGT TGA GCA GGA CCC TGA GA 3’, and a reverse primer 5’ CCA GTC ACC ACA GAG CGT TA 3’. The PCR products were further sequenced and analyzed to see variations of the MC1R gene of all cattle. The results indicated that the sequence of MC1R gene in the 10 Bali cattle has a nucleotide variation of 0.31% generated from Bos taurus and 0.21% generate...
Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3
...from the samples using a kit , and PCR amplification was done using ITS4 and ITS1F primers designed from the conserved regions as described in the previous studies. The amplified PCR products were sequenced from Macrogen Korea, via worldwide scientific Pakistan. Phylogenetic analysis of the obtained sequences was done based on the maximum similarity method using Mega 6.0. The spore was smaller as compared to Marasmiellus scandens (6.2-8.7 µm)...
Irtaza Hussain1, Muti ur Rehman Khan1*, Asim Aslam1, Masood Rabbani2 and Ahsan Anjum1
...s were detected by ELISA kits up to 50th day post-infection in the current study. This is the first study that reported the seroprevalence of contagious ecthyma in Punjab, Pakistan. Comprehension of risk variables will not only be helpful to develop awareness amongst farmers but will also provide guidelines to government officials about the prevention and control of the disease....
Kandiah Pakeerathan*, Konesalingam Jeyavithuyan, Aruchchunan Nirosha and Gunasingham Mikunthan
...ze leaves), sawdust, and kitchen waste were used as mushroom substrate and then mushroom grown waste was converted into bio-fortified vermicompost using exotic earthworm Eisenia foetida+ Trichoderma viride + Pseudomonas fluorescens. Onion growth parameters, yield, and DSI were recorded from the experiment conducted in a complete randomized design and subjected to ANOVA using SAS 9.1. Turkey’s HSD multiple comparison test and Person-correlation analysis w...
Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3
Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8
...h commercially available kits. The current study resulted that selenium supplementation improved(P≤0.05) the catalase level in the liver and muscles which fight against the free radical and reduce oxidative stress. In the blood serum the catalase and Malondialdehyde (MDA) both shows significant (P≤0.05). In conclusion I assume that 0.3mg/kg of selenium supplementation in redox status and thyroid hormone show better results in comparison to the control gr...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...evelopment of diagnostic kit against hemorrhagic septicemia (HS) because of its immunogenic nature. Pasteurella multocida has economic importance because of endemic and epizootic diseases in domestic and wild animals. This bacterium is an opportunistic pathogen of upper respiratory tracts of wild and domestic animals and birds as well as in livestock . The ompH gene was amplified with in-house designed primers, the amplified gene (1002 bp) was cloned into pET ...
Iko-Ojo Charity Ikwe Agada, James Agbo Ameh, Olatunde H. Olabode and Martha Echioda-Ogbole*
...ovirus antigen detection kit (IDEXX Laboratories, United State). Other disease conditions include helminthiasis 25 (12.7%), canine babesiosis 3 (1.5%), while 37 (18.78%) cases were recorded as canine distemper and other bacterial enteric diseases. Monthly distribution of CPE cases in this study showed significant difference (p<0.05) characterized by high prevalence in the dry season with peak of infections in the month of January. Breed disposition to CPE s...
Morcos Ibrahim Yanni1* and Eid Elsaid Abdelaziz2
...ccinal strains (Evelyn-Rokitnicki-Abelseth, Pasteur, the China human rabies vaccine strain AG and others at this study) due to the presence of mutation sites. As the P gene plays a role in viral pathogenicity, the detected variance in nucleotide and amino acid identities of the P gene in our local circulating isolates over the years confirmed the anticipation of distinction in the pathogenicity of such isolates. All vaccinal strains have a genetic distance fro...
Ibrahim l , Madiha Salah and Ikuta 2, Kazuyoshi
...r Sysmex avian influenza kit and 86% and 33% for Sysmex influenza MB kit. Thus, Sysmex avian influenza kit was shown to be a potentially useful tool for the direct and rapid detection of H5N1 in clinical specimens due to its high specificity. Additionally, an advantage over the other kit is specifically reacting with viruses of avian origin but not human...
Hassanein, Suzan A.; Abd El-Wahab, Wafaa; Eweis, Moustafa and Mahmoud, Mervat M.
...s using commercial ELISA kit (Priocheck). The overall percentage of positive was 38.9 %. The higher percentage of positive detected in Behaira (48%), then Mounofya (45.3%) while Kafer El-sheikh was the lowest (23.7%). The positive results of detection of antibodies against non structured proteins of FMDV indicate that these samples come from natural infected animals....
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
...C using commercial ELISA kit (Prio-check) for l065 serum samples were collected (735) from Sharkia and (330) from Kafr ElSheikh Governorates during 2009 from cattle and buffaloes. The higher percentage of positive was detected in Sharkia (31.7%) while Kafr Elsheikh was (27.3%). The positive result of detection of antibodies against non structured proteins of FMDV indicates that these samples come from natural infected animals. The further work is required to e...
Abo Elmagd l , Enas K.; Abdel-Wahab l , Kouka S.; Alrasheedy l , Zeinab E. and Khalifa2, Ahmed S.
...ission. Commercial ELISA kits for IgG, IgM, IgA anti-HCV epitopes and RT-PCR kits were used according to kit instructions. Dot ELISA for detection of HCV antigen in infants PBNINC lysates was an in house test. Previous exposure to HCV infection indicated by antibodies to the HCV six epitopes was detected in 8/61 (13%) mothers. Active HCV infection by anti-core and HCV-RNA detection was 5/8...
Amer, H.M.; Hussein, H. A.; El-Sabagh, I. M.; El-Sanousi, A. A. Saber,M.S. and Shalaby, M. A.
...using DNA gel extraction kit (Millipore). Eluted DNA was successfully cloned in pBlueBac4.5N5-His TOPO TA baculovirus transfer vector and transformed in chemically competent E. coli. A modified colony PCR assay was utilized to identify the positive bacterial colonies that harbor the recombinant plasmids carrying N gene in correct orientation. Generation of recombinant baculoviruses was achieved by co-transfection of Spodopetra frugiperda (Sf-9) insect cells wi...
M. E. Shawky and A. M. Daoud
...bent assay (ELISA) based kit for detecting foot and mouth disease (FMD) antibodies using a native FMDV strain (O1. Aga 93). Results were compared to those of serum neutralization test (SNT) and 3CD ELISA Kit. Sensitivities of the ELISA Kit and 3CD ELISA were 94% and 91%, respectively. Rapid confirmation of the presence of FMDV antibodies through the developed ELISA Kit may have a significant impact on initiating emergency control of FMD outbreaks. ...
Wisnu Jaka Dewa1,2, Ekowati Handharyani3*, Sri Purwaningsih4, Silmi Mariya5
...cells using a commercial kit. The mRNA concentration was then measured with a UV-Vis microvolume spectrophotometer at 260/28 nm. Relative expression of bax and bcl-2 to house keeping gene GAPDH was measured on a real time PCR machine. Relative gene expression was calculated using the ΔΔCt method. The result showed that bax gene expression increases 4.36, 4.77 and 4.63 times in 125, 62.5 and 31.25 ppm concentration, respectively. The expression of b...
Joseph Oyiguh Abraham1, Cornelius Arome Omatola1*, Martin-Luther Oseni Okolo1, Ruth Foluke Aminu1, Emmanuel Edegbo1, Olubunmi Marvelous Emurotu1, Danjuma Muhammed2, Jesse Joseph Chock3, Helen Ojomachenwu Ocean4 and Yahaya Ocholi5
...mercial immunoassay test kits. Relevant demographic information was obtained from each participant with a structured questionnaire. Of the 1000 participants, HBV, HCV, and HIV were detected in 28 (2.8%), 2 (0.2%) and 3 (0.3%), respectively. However, no participant was simultaneously infected with any two or three viruses. The age group 21-40 years demonstrated greater exposure to HBV (7.57%), HCV (0.37%), and HIV (1.17%) infection than the others. More male th...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Vincentia Trisna Yoelinda1, Sarmin1, Yuda Heru Fibrianto1, Pudji Astuti1
... assessed using an ELISA kit, and the acquired data were tested for normal distribution and homogeneity, followed by analysis through the T-test method. The results indicated an increment of testosterone and corticosterone hormones among experienced Stone Magpie birds after the contest; however, these changes did not reach statistical significance. The mean values, both before and after the contests, of testosterone levels in the amateur group did not show a s...
Thobela Louis Tyasi*, Lebo Ngorima, Victoria Rankotsane Hlokoe
Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati
Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9
...sured through commercial kits. The result of the current study showed that the values of Alanine transaminase (ALT), Aspartate aminotransferase (AST) and Alkaline phosphatase (ALP) were higher significantly (P≤0.05) in negative control group (B) as compared to the group C, D and E. The level of uric acid, creatinine and blood urea were significantly higher (P≤0.05) in group A (negative control) (P≤0.05) as compared to the group B (positive control) C,...
ZARYAB KHALID SIAL & FARAH KHAN
Waseem Abbas, Imtiaz Ahmed* and Imran Khan
...by Megazyme starch assay kit. A nine-point hedonic scale assessed bread samples for consumer acceptance. The findings of proximate composition showed a significant increase in ash at 2 and 3 % incorporation. The fiber content also significantly increased at the incorporation ratio of 1, 2 and 3%. In CWB, fat concentration at 2 and 3% incorporation remained almost equal to that of CB. The protein content increased “significantly in a dose-dependent manner...
Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4
...CS using CD34+ selection kit. CD34+ cells were differentiated in to EPCs by using erythroid expansion supplement. Purity of the CD34+-derived EPCs was assessed through immune fluorescence microscopy using monoclonal antibodies against erythroid specific surface antigens CD34, CD235a and CD71. The purity of CD34+-derived EPCs was determined to be 95%-98%. Cord blood samples contain immature cells, which can result in contamination of PBMNCs layer. Thus, obtaini...
Ahmed Kareem Kadhim Al-Wasmee1, Waddah Salam Hassone2, Hawraa Talib Al-Janabi3, Hamed AH Al-Jabory1
...ructions provided in the kit to extract the parasite DNA. The results revealed that the rates of infection of calves with T. anullata in Babylon Province were 36/90. The infection rate of T. anullata was recorded for females (47.5%) and males (34 %). Results showed that the highest infection rate (62.5%) was recorded at the <1 M, (39.1%), at 1-2 M, (20%) at 2-3 M, (22.7%) at the 3-4 M, and the lowest infection rate (14.3%) was recorded at the ≥4 M. The i...
