Sania Subhan Qureshi, Mian Saeed Sarwar and Zahir Shah
...agnosed a case of muscle tumor (myoma). A skin incision was given to expose and to remove tumor mass from the surrounding tissues. Peritoneum, muscle and subcutaneous tissues were sutured with absorbable suture (vicryl; polyglactin 910) and skin with a non-absorbable suture (silk). Owner was advised for daily check-up for any damage to the stitches and for regular dressing of the wound with antiseptic. Antibiotics and analge...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...to promote cell death in tumor cells when produced endogenously in the cells or applied exogenously. Ceramidases, which are main component of ceramide pathway catalyze metabolism of proapoptotic ceramide to anti-apoptotic sphingosine and other fatty acids. In this study we have evaluated the pro-apoptotic effect of ceranib-2, a human ceramidase inhibitor and of a short-chain C2 ceramide on human lung adenocarcinoma cells, A549 by determining cytotoxicity of th...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
...ed birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup...
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...cal conditions including tumor development. The most important factor implicated in the angiogenic processes is vascular endothelial growth factor (VEGF) protein and its family of ligands and receptors (VEGFRs). Survival, proliferation, migration and invasion of endothelial cells which give rise to the vascular network, is mediated through VEGF/VEGFR activation of the signal transduction pathways. Thus blocking VEGF is an efficient strategy for blocking
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
...ferent canine and feline tumors due to its predetermined role in tumor pathogenesis. A total of 52 tumor and normal samples were examined, among these, 40 canine and 12 feline samples were analyzed using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...sed the concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection (P<0.05). However, there were no apparent changes in organ index or the relative expression of TLR4 and IgM (P>0.05). Furthermore, correlation analysis showed a positive rel...
Riffat Mehboob1*, Shahida Perveen2, Naseer Ahmed3
... lipoma, 5 of phylloides tumor (PL), 4 of fibrosis (F), 2 of intraductal papilloma (IDP) and 3 of accessory breast with Fibrocystic (FC) changes. The relation between the ages and frequency of the different types of lesions was also analyzed to understand the association between predisposing factors and the nature of lesions.
Conclusions: These results demonstrated that the FA is the most frequent benign breast lesions and is common am...
Farhat Ijaz1, Rana Khurram Aftab2*, Samia Jawed3
...nterleukins 6 (1L-6) and tumor necrosis factor alpha (TNF-α). Relation of TNF-α with obesity induced IR and T2DM is unclear as results obtained from different studies are very controversial.
Objective: This study was designed to compare TNF-α levels and insulin resistance in obese and non-obese type 2 diabetics.
Methodology: A cross sectional comparative study was conducted in diabetic clinic of Mayo Hosp...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
...he most common malignant tumor in children and forms a major fraction of childhood malignancies in the developed countries. Numerous chromosomal aberrations have been observed in ALL including translocations which result in the production of fusion genes (BCR-ABL, TEL-AML1, and MLL-AF4). These chromosomal rearrangements have great prognostic and therapeutic significance. Amongst these, TEL-AML1 fusion gene represents a subgroup of ALL cases, which are a...
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
...s have reported the anti-tumoral properties of statins by exhibiting anti-proliferative properties in HMG-CoAR positive breast carcinomas. Increased efficacy has also been observed when these were used as combination therapy along with tamoxifen in estrogen receptor (ER) and HMG-COAR positive breast carcinomas. However, in our local population, expression of HMG-COAR is not investigated. The objective of our study was to examine the expression of HMG-CoAR in p...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
... and metastasis. ROS and tumor cell interaction could activate the signalling pathways, promoting cell proliferation, invasion, inducing angiogenesis, inflammation and cellular transformation in cancer. A better understanding into the mechanism of ROS in cancer progression might be useful for the development of biomarkers and therapeutic strategies. The objective of this review was to summarize the roles of ROS in different stages of cancer, cell invasion, ang...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...teral limbus of eye. The tumors were large and usually protruded through the palpebral fissure and infiltrate the entire eye ball. Diagnosis was made based upon the clinical and histopathological examination of the tumor sample. In all cases, tumor resection was performed followed by cauterization of the remaining surface with silver nitrate. No postoperative complications or local recurre...
Amna Younus1,3, Mariam Faiz2 and Abida Yasmeen1,*
...oma (IDC: 88%), grade II tumor type (47%), -ve estrogen receptor/ progesterone receptor (-ER/PR: 35%) and negative human epidermal growth factor receptor 2 (-ve Her2: 53%) hormonal status. Results also depicted that FokI and TaqI genotypic frequencies were significantly (P<0.001) distributed among women with different tumor grades and ER/PR status. FokI genotypic distribution was significantly associa...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
... line treatment for some tumors. The present study was aimed to explore the cellular targets and anticancer mechanism of piceatannol in human osteosarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase ar...
Huma Sattar1, Sehrish Firyal1,*, Ali Raza Awan1, Habib-Ur -Rehman2, Muhammad Sajid Hasni3 and Amjad Islam Aqib4*
...f polymorphism in bovine tumor necrosis factor alpha (TNF-α) gene on immune function and its role in mastitis susceptibility in Pakistani Sahiwal cows. In this study, a total of 150 Sahiwal cows were selected suffering from clinical (n=50), subclinical (n=50) mastitis along with non-mastitic (n=50) cows from different dairy farms of Punjab. TNF-α gene was amplified with specific primer and sequenced to get the full-length sequence of ...
Hafiz Muhammad Tahir*, Palwasha Jabeen, Chand Raza, Shaukat Ali
... been hailed to have antitumor activity and has also been widely used in different cosmetics. The major hindrance in using spider silk for different purposes is due to its small obtainable amount. Efforts are being made to overcome this problem by modern biotechnological techniques including of making transgenic organisms.
...
Hafiza Sadaf Zahra1, Asia Iqbal2, Sayyeda Hira Hassan1, Hafiz Abdullah Shakir1*, Muhammad Khan1*, Muhammad Irfan3, Chaman Ara1, Shaukat Ali4
...sults in inactivation of tumor suppressor genes (TSG), while in hypomethylation, demethyltransferase enzyme causes the activation of oncogenes by promoting transcriptional activity. Contrary to DNA methylation, histone acetylation and deacetylation results in chromatin opening and closing, respectively; leading to transcriptional activation and inactivation of genes. Histone acetylation has been frequently detected in oncogenes while histone deacetylation in T...
Rimsha Munir1, 2*
...es when cultivated as 3D tumor spheroids. The presented work aimed to study the interplay between cell proliferation rates and metabolic stress in 2D and 3D cell culture systems. Two cell line models (HepG2; liver cancer cell line and HCT-116; colorectal cancer cell line) were selected that showed inverse proliferation trends under 2D and 3D cell culture systems: HCT-116 displays decreased, while HepG2 displays increased proliferation rates under 3D system. Th...
Sameh S. Tawfik, Ahmed A. Elkady* and Ehab T. Mohamed
...d the elevation in serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels when compared to irradiated rat group. The histopathological-findings of renal tissues in the irradiated-group, showed nephrotoxicity; the renal cortex showed massive necrotic changes, the convoluted tubules showed distinctive pattern of ischemic renal injury and the cuboidal epithelium cells of proximal and distal renal tubule...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...period. Plasma levels of tumor necrosis factor alpha (TNF-α) and alpha-fetoprotein (AFP) were estimated before and after the administration of different meals. After the experiment, the liver samples were weighed and histopathologically evaluated using a knodell score to assess portal hypertension and liver damage. Animals fed with commercial chicken meat and feed for six weeks showed development of inflammation, necrosis, apoptosis and cirrhosis on hist...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...ce gene (MDR1) in common tumor malignancies, and evaluate the prognostic value of MDR1 expression in common malignancies. mRNA relative expression levels of MDR1 were detected byreal-time quantitative polymerase chain reaction (RT-qPCR) in tumor, adjacent, and non-cancerous tissues. The tumor markers were detected with COBAS 6000. The prognostic value of relative MDR1 expression level in m...
Jing Zhang1,2, Xin Wang2, Yun Zhao2, Yongcheng Jin2, Yongfeng Zhou2, Junmei Wang2, Yurong Fu2, Rui Wang2, Ruihua Li2, Hengtong Fang2 and Hao Yu2,*
...gamma (Reg3γ), and tumor necrosis factor (TNF) were significantly downregulated. The mRNA expression levels of mucosal β-defensin, regenerating islet-derived protein 3 alpha (Reg3a), regenerating islet-derived protein 3 beta (Reg3β) and secretory immunoglobulin A (sIgA) levels were significantly increased. The interleukin-1beta (IL-1β) level was decreased. 16s RNA sequencing further indicated that the colonic microflora had changed, especi...
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...ent of various malignant tumors, but it is accompanied by severe body toxic reactions, especially cardiac toxicity. This article explores the effects of pilose antler peptide (PAP) on DOX-induced myocardial injury and related mechanisms through in vitro experiments. Different concentrations of DOX were used to treat H9c2 cells for 6, 12, 24, and 48 h, respectively, to determine the IC50. Different concentrations of PAP were treated to H9c2 cells for...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
...its chemopreventive anti-tumor properties. Aspirin is considered to be partially mediated by the induction of apoptosis in cells. However, the underlying molecular mechanisms of aspirin in the prevention of cancer are yet to be fully elucidated. In the current study, the GSE115660 microarray dataset was downloaded from the Gene Expression Omnibus database to identify key genes in aspirin-damaged yeast cells following redox injury. Differentially expressed gene...
MA Liman1, QI Yongxiao1, Wang Wenji2* and Zhong Qianyi3*
...g promoting uncontrolled tumor growth. Inhibiting angiogenesis using natural compounds is an important focus in developing new cancer treatment strategies. Ginsenoside Rh2 (G-Rh2), a natural product derived from red ginseng, has been reported to have therapeutic effects on some tumors, but its antiangiogenic effects in zebrafish has not been evaluated. Here we investigated the antiangiogenic of G-Rh2 using the zebrafish angi...
Mehwish Saleem Khan
Evaluation of Changes in Zinc Levels in Patients Suffering from Cancer
..., blood cancer and brain tumor. In addition to serum zinc level of cancer patients many other parameters were also studied such as, age limit, weight of cancer patients, effect of smoking on cancer patients, effect of radiation on cancer patients, effect of chemicals on cancer patients, infections in cancer patients and physiological changes in some patients. The experimental work had been done in city lab and research center Lahore to evaluate the serum zinc ...
Humza Sami1, Mahnoor Sagheer1, Muhammad Amir Altaf1, Javaid Iqbal2 and Muhammad Zubair1,*
...reconstruction of breast tumor images. An UWB Vivaldi antenna is used in the bandwidth of 2-6 GHz to detect and localize the small tumors. The prototype hardware setup uses two Vivaldi antennas, one as transmitter and other as receiver for scanning. The distance between phantom and antennas is kept almost 20 cm. The scattered signals from the high contrast objects emulating the tumor chara...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...acellular space of solid tumor interstitial fluids. The intracellular AGR2 is critical in protein folding while secreted AGR2 function as a paracrine signal promoting tumor microenvironment formation. Beside cancer progression and drug resistance, the abnormal expression of AGR2 is also involved in diseases like asthma and inflammatory bowel disease. Studies on the role of AGR2 in tumor mi...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...ely) and increased serum tumor necrosis factor alpha (TNF-α) concentration (P <0.01), but ACTH treatment has no significant effect on them. Neither ACTH nor LPS treatment had any significant effect on levels of pulmonary SOD, CAT and MDA secretion, as well as expression of IL-6, TNF-α, IL-10, COX-2 and TLR4 mRNAs (P >0.05). Pulmonary cortisol level was inhibited by LPS (P =0.034), with a similar trend of inhibition by ACT...
Ho-Seong Cho1 and Yeonsu Oh2,*
...s severely affected by a tumor, and small tumor nodules were disseminated throughout the other lobes. The numerous tumor nodules were also found in the lung, stomach, kidney, heart and diaphragm, which were growing together, suspiciously metastatic, projecting, 5 to 40 mm in diameter and tawny to white in color. Histopathologically, the tumor was compose...
Muhammad Abdullah1, Sana Zahoor1*, Akhtar Ali2 and Masroor Ellahi Babar3
Furqan Awan1,2, Sadia Nawaz3, Muhammad Muddassir Ali3,*, Asia Iqbal4, Raheela Akhtar5, Sehrish Firyal3, Haleema Sadia6, Arshad Javid4, Muhammad Rashid7, Muhammad Sulman Ali Taseer8 and Zhenling Zeng1
...e transmissible venereal tumor (CTVT) in pet dogs; and identify the potential factors that are playing important role in disease spread. A questionnaire-based study was conducted to identify the conditions and circumstances of CTVT affected dogs that presented to veterinary practices over a 12-month period. The practices were convenience-sampled on the basis of voluntary participation. Forty practices were approached, and veterinarians from 24 practices agreed...
Jing Fu
...oup) in mucinous ovarian tumors and their correlation with tumor pathological classification and prognostic outcome. One hundred and sixty cases of ovarian mucinous tumors diagnosed from January 2018 and January 2020 were selected. They were divided into four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82&plu...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
...appa;B (NF-κB) and tumor necrosis factor α (TNF-α). The elevated levels of Creatine Kinase - MB (CK-MB), Lactate Dehydrogenase (LDH), and Troponin-1 induced by doxorubicin injection were neutralized by almond oil supplementation. Almond oil ameliorated all the histological alterations caused by doxorubicin. The administration of almond oil with doxorubicin modulated the doxorubicin-induced changes in serum and cardiac tissue in mice due to it...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
... extracts. Also, the antitumor activity of the scaffolds was studied against cancer osteosarcoma cell lines using Sulforhodamine B (SRB) assay. The results showed that addition of cerium-doped nanobioactive glass to the composite scaffolds was triggered an increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO2 innanobioactive glass (sample CL...
De Hu, Jia Wang, Huan Wang, Xiang-Yang Leng, Tian-Yi Zhao* and Shu-Min Wang*
...tissue and the levels of tumor necrosis factor (TNF–α) and interleukin (IL-6) in serum were measured through ELISA, and the contents of NLRP3, ASC, caspase-1 and IL-1β in gastric tissue were detected by Western blot. The results showed that FP of C. lilacina could effectively prevent the stress gastric ulcer induced by WIRS in mice by reducing the expression level of NLRP3/ASC/caspase-1....
Seun Eui Kim1,2*, Myoung-Hoon Lee1, Hye Myoung Jang2, Garam Park2, Wan-Taek Im3, Gwang Joo Jeon2,4*
...stoma is the most common tumor in children and is still a carcinoma, which requires discovery of new therapeutic agents as were in cases of other cancers. Although the anticancer activity of a rare ginsenoside compound K (CK) against various cancers has been reported, there are few studies on neuroblastoma. In this study, cell viability and cell migration assay were tested and the expression of 17 genes in related signaling pathways was analyzed to determine t...
Asmaa Khamis1, Osama Abdalla1, Mohamed Hashem2, Noha Abdelnaeim1*
...sed MDA, caspase-3, p53, tumor necrosis factor-α, interleukin (IL)-1β, IL-18 and, IL-10, and a significant decline in glutathione and superoxide dismutase in the CCl4 group. However, intoxicated groups treated with PN and PM showed marked improvement in the measured parameters. Therefore, PN and PM have antioxidant and anti-inflammatory effects, especially PM, which showed better improvement than PN against CCl4 hepatotoxicity in rats.
&...
Neeranoot Detcharoenyos1, Nakrob Pattanapon1, Soontaree Petchdee2*
...mmon type of benign bone tumor. We report here a rare case of osteochondroma in a 4-month-old male golden retriever dog presented to Kasetsart Veterinary Teaching Hospital, Kamphaeng Saen, with progressive paresis over two weeks. The dog was ultimately diagnosed with a spinal tumor by radiography and MRI. In this study, the dog was successfully treated by dorsal laminectomy of T8-T9 to excise an abnormal spinal mass, and a m...
Rawda S. Mohamed1,2, Mohammed Y. Matoock2*, Abeer H. Abdelrazek2
...of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), and glutathione (GSH) were measured. Mice were subjected to open field, plus maze, and dark light activity box tests. Swiss Multiple Acute concurrent Stress (SMAS) showed more responsiveness to MAS than Balb/c Multiple Acute concurrent Stress (BMAS) in most biochemical and behavioral parameters, with an elevated level of corticosterone and MDA IL-6, and TNF-α and r...
Tariq Mehmood1, Muhammad Arif Khan1, Ayesha Safder Chaudhry1, Kamran Ashraf2, Muhammad Mohsin Ali1,3, Muhammad Faisal Ayoob3*, Mudassir Hussain4, Farrukh Saleem3 and Muhammad Fayaz3
...s blockage, peritonitis, tumors and diverse local injuries and lesions are corrected by resection of the intestine. Twelve cats were subjected to 3 variable degrees of jejunal resection, i.e. group A (20%) and group B (40%), while group C (0%). Sampling was performed on days 0, 15, and 30 to test various clinical and biochemical parameters. Results showed weight loss in all groups until day 15 post-surgery while in group A and C gradual weight gain was noted. ...
Zhaojun Wang1, Yang Zhou2 and Xinghua Song3*
...interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were detected using ELISA method before treatment and 3 months after treatment; incidence of adverse prognostic reactions was compared between the two groups. After treatment, the observation group had 34 markedly effective cases and 21 effective cases, with total effective rate of treatment at 91.67%. The figure was significantly higher compared to 80.00% of the control group, showing...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...gulate the expression of tumor suppressor genes in a variety of cancerous cells. The present study explores the anti-cancer activity of UNC0642, a novel potent inhibitor of G9a in MCF-7 breast cancer cell line. Analysis of growth and proliferation parameters by various cell-based assays such as neutral red assay and BrdU cell proliferation assay showed that higher concentrations were lethal for the cancerous cells. IC50 was found to be 12.6 µM. Further c...
Qi Zhuo, Yuanchun Yao, Meisongzhu Yang*, Jinhua Chen and Miao Tian
...cer. MiR-216b-5p has antitumor effect on HDAC8 and can inhibit the proliferation and development of breast cancer....
Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3
... genes in the BC and FCM tumor tissues, as well in the uterus tissue and WBCs using qPCR. Results. In cats with MG tumours, signs of lymphopenia and thrombocytopenia were found, these being especially pronounced in animals with BC. In cats with BC and FCM, there were significant differences in the expression patterns of the spectrum of target IFN system genes and a number of key cell cycle control factors. These differences were expressed in the tumour tissue ...
Xiajun Zhang1, Jie Yang2, Wenjun Zhou1, Zhenshi Chen2, Weidong Wu3, Shaoru Zhang2 and Lihui Wang2*
... gynecological malignant tumor in many countries. TGF-β regulates cell proliferation, angiogenesis and metastasis, and is an inducer of epithelial-mesenchymal transformation. Tumor cells activate the TGF-β/Smad pathway, and inhibiting its signal transduction pathway is an important strategy for tumor therapy. First, we have found lncRNA CASC7 is a poor prognostic marker of endome...
Man Wang1 and Fengmei Yang2*
...OP2A gene and protein in tumor tissues were higher than those in normal tissues, and the differences were statistically significant (P<0.05); TOP2A gene expression was positively correlated with immune cells infiltration (P<0.05); the overall survival, disease-free survival and survival probability of patients with high expression of TOP2A gene were lower than those with low expression of TOP2A gene (P<0.05). In clinical specimens, the expression of T...
Mingqing Wang1 and Xiaojuan Chen2,*
...or these patients. Also, tumor necrosis factor-alpha (TNF-a) was assessed as an inflammatory factor to see the response to treatment. In this study, 180 AECOPD patients who had been treated in our hospital were enrolled as research objects, who were given routine respiratory stimulant therapy regimen and intravenous methylprednisolone. According to the dosage of glucocorticoid methylprednisolone, the patients were divided into three groups, each containing 60 ...
Xuan Liang, Rongfang Zhang, Yan Xu, Hui Yang, Li Guo, Feiyang Na and Donghai Liu*
Zongliang Li1, Rongxin Ma2,*, Yan Wei1 and Jianghua Zuo3
... 30 (75.00%) had a lower tumor growth rate (P<0.05) than the combination treatment group (92.50%), and the combined treatment group 32 (80.00%) had a significantly reduced tumor depth compared with the conventional treatment group 21 (52.50%). There was no difference in the rate of bronchospasm between the conventional treatment group and the combination treatment group (P>0.05). 32P application combined with timolol m...
Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1
... the Ehrlich inoculation tumor growth was confirmed: control, Cis (5 ml/kg), Pio low dose (LD) (17.5 mg/kg), Pio high dose (HD) (30 mg/kg), Cis+Pio LD, and Cis+Pio HD. Different parameters, including behavioral, biochemical such as malondialdehyde (MDA), reduced glutathione, superoxide dismutase (SOD), tumor growth factors, and apoptotic TUNEL assay, were evaluated after three weeks in addition to Immunohistochemical analysi...
Caiping Duan
...interleukin-6 (IL-6) and tumor necrosis factor-α (TNF -α) were detected by Western blotting (WB). The protein contents of B-cell lymphoma factor 2 (Bcl-2), apoptosis-related genes Bax and caspase-3 were detected by WB. The contents of lactic dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Results showed that compared with the control group, the low-d...
Yuhua Chen1 and Song Lu2*
... acupoint stimulation on tumor-related T cells. C57BL/6J mice used in the study were randomly divided into control and electrical stimulation group. The tumor model was established by subcutaneously injecting MC38 colorectal adenocarcinoma cells into mice. The mRNA expression of pro-inflammatory factors IL-10, IL-6 and 1L-1β of the mice was analyzed by RT-PCR. The numbers of CD8 + T cells and CD4 + T cells were analyzed...
Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1
...rkable inhibition in the tumor suppressor miRNA (let-7a) compared to DOX-induced expression. Moreover, miR-122, as negative regulator of DOX resistance, showed a dramatic induction in CSO/DOX group compared to DOX group. Conclusion, CSO is capable of effectively inhibit DOX resistance, induced hepatic, nephro and cardiac antioxidant status, inhibit lipid peroxidation, and restore hepatic function.
...
...
Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin
...ground: Sex cord-stromal tumors of the ovary are a rare and heterogeneous group of benign and malignant neoplasms with a wide range of histological characteristics. The current study investigated ovarian sex cord-stromal tumors in dromedary camels slaughtered at Egyptian slaughterhouses between January 2019 and February 2022. The study included 180 female single-humped camels in which all data on the animal’s age, loca...
Amr N. El-Shahat1*, A.M. Abdul Azeem1 and Mohamed H.M. Abd el Megid2
...ipoprotein-cholesterol), tumor necrotic factor-alpha and interleukin-6 as well as reduced activity of hepatic and cardiac enzymes. Also, feeding rats on HCD with BLE enhance the antioxidant status in liver and heart tissues with indicated inhibition of lipid peroxidation by reducing the level of malondialdehyde compared to HCD-fed rats. The results concluded that BLE may have an effective role in reducing high cholesterol levels....
Muhammad Nasir Bhaya1,2* and Hikmet Keles2
...reat different malignant tumors. Nephrotoxicity is one of the major side effects of it. Ellagic acid (EA) is a polyphenol, known for its antioxidant properties. In this study, the protective effect of EA on CP-induced renal damage was evaluated. For this purpose, 24 rats were divided into four groups, control, CP, CP+EA50, and CP+EA75. Histopathological examination of kidneys revealed hyperemia, epithelial degeneration, swelling, cystic dilatation and luminal ...
Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*
...and lethal primary brain tumors due to its high malignancy and heterogeneity. Despite recent advances in medical technology and concerted effort to improve therapeutic methods, the prognosis of GBM still remains very poor. The poor prognosis is associated with cancer stem cells (CSCs) which hold the potential of self-renewal, and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistanc...
Weijun Chen1*, Shiyang Gao1, Ping Sun1, Xiaohui Sun2, Yongbin Wei1, Jingyang Chen1 and Jialong Chen1
...d the mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and IL-6 in liver of common carp compared to control diet (P<0.05). However, diet NaBT significantly improved fish liver health by decreasing contents of TCHO and MDA, down-regulating mRNA expression of pro-inflammatory cytokines (e.g., TNF-α, IL-1β and IL-6), increasing the activity levels of antioxidant enzymes, and upregulating the mRNA expr...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...dant enzyme activities), tumor necrotic factor-α (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage and remarkable improvement in all the measured parameters when compared to BPA-rats. In this study it was concluded that the synthesis of selenium nanoparticles using a natural source such as grapefruit was effective in combining the antioxidant and anti-...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...dant enzyme activities), tumor necrotic factor-α (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage and remarkable improvement in all the measured parameters when compared to BPA-rats. In this study it was concluded that the synthesis of selenium nanoparticles using a natural source such as grapefruit was effective in combining the antioxidant and anti-...
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
...bserved. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH) and reduced glutathione peroxidase (GSH-Px) were detected by ELISA. The expression level of TLR4/NF-κB, and HandE staining was used to observe the renal tissue. In the results, compared with model group, the spleen index and whole blood viscosity was significantly dec...
Azza M. El-Kattawy1, Tarek Abou Zed1, Randa Megahed1 and Mohammed El-Magd2*
...lammation-related genes [tumor necrosis factor alpha (TNFα) and interleukin 1 beta (IL1β)], 3) declined the expression of matrix metalloproteinase-3 (MMP3), transforming growth factor beta (TGFβ), and oxidized-LDL receptor (OLR1), 4) upregulated the expression of the anti-inflammatory gene IL10, 5) reduced the levels of the oxidative markers [lipid peroxide marker malondialdehyde (MDA) and nitric oxide (NO)], 6) increased the levels of antioxid...
Wei Wang1, Meifeng Zhou2, Yuebo Liang1, Fan Zhang1, Zhong Wu3, Shaowei Mo4* and Yi Qing Wang1*
...icantly increased in the tumor tissues of HER2-positive breast cancer patients. Consistently, the expression of YAP and TAZ were both dramatically upregulated in SK-BR-3-TR cells. The cell viability was increased, while cell apoptosis was inhibited in SK-BR-3-TS cells compared with SK-BR-3-TS. The depletion of YAP by si-YAP reversed the YAP/TAZ expression, cell viability and cell apoptosis in SK-BR-3-TR cells. YAP/TAZ pathway might induce the trastuzumab resis...
Mahmoud Abdelhady Metwally1*, Mona Abdelftah Ali1, Farouk Abdelmohdy1, Mohamed Kassab1, Tarek Kamal Abouzed2 and Khalil Fathy Abou-Easa1
...targets of BMMSCs-CM for tumor cell suppression....
Qiangsheng Wu1, Yumei Yin2, Hui Gao1, Baoding Chen3, Jianjun Yu2, Jian Zhang4, Donglai Zhu5, Fang Liu6, Guohong Ge7 and Ya-Mei Dang8*
...arcinoma patients’ tumor tissues were compared to 8 normal liver tissues to screen out the significant abnormal expressed genes. The potential interactions and mechanisms of these genes in promoting hepatocellular carcinoma were analyzed through bioinformatics analysis. The results showed that the functions of these 1495 differently expressed genes enriched in cell cycle, oxidation-reduction and drug metabolic process. Additionally, the top 10 core genes...
Sidra Mumtaz1, Sidra Arshad1, Rizwan Ullah Khan1, Muhammad Usman Rashid2 and Naila Malkani1*
...ion in various stages of tumor formation in breast tissue. However, their use as a prognostic factor is not very clear. Therefore, a meta-analysis was performed to analyze their aberrant expression in breast tumorigenesis. A thorough literature search was performed and 73 relevant studies were identified which were further scrutinized and finally 26 studies covering 4553 subjects were included for analysis. A random effect m...
Qi Jiayi1, Huang XuMiao2, Lin Wei3, Ao YaHui3, Zhao Wenting2* and Cao Chunhao3*
...lay an essential role in tumorigenesis and progression, and sought to develop an immune-related lncRNA signature to assess the value of immune-related lncRNAs to discover their prognostic values. RNA-seq and clinical information were obtained from the Cancer Genome Atlas (TCGA) database, which aims to screen for immune-associated lncRNAs based on constructing a risk scoring formula. Besides, in virtue of univariate and multivariate Cox regression, immune-assoc...
Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2
...(VLDL)-c concentrations, tumor necrosis factor-alpha (TNF-), and interleukin 10 (IL-10) concentrations. The results revealed the development of signs of incitement such as increased TC and reduced IL-10, decreased HDL-C, and increased TC, TAG, VLDL-C, and LDL-C, along with an increase in inflammatory markers. CPNPs reduced dyslipidemia and inflammation in female rats. We implied the protective impact of CPNPs, which may be seen in the anti-inflammatory and hyp...
Zhi Qiang Gao*, Dan Dan Zhang, Qin Gmei Qin, Xiu Xiu Li, Li Li, Yan Zhong Xue and Shifeng Guo
...gnosed with glioneuronal tumors (GNTs), including 25 cases of gangliogliomas (GGs) and 15 cases of dysembryoplastic neuroepithelial tumors (DNTs). Then, the DNA of GNTs tissues was extracted, and the PSEN1 E280A mutation was detected by sequencing. Finally, the correlation of PSEN1 E280A mutation with clinical characteristics was analyzed. PSEN1 E280A mutation was detected in 16 GNTs patients (44% in GGs (11/25) and 33.3% in...
Qingsen Ran1,2, Manjing Li3, Jiayin Han3, Lifang Wang3, Han Wang3,4, Shaobo Liu5* and Yanping Wang1*
...nvasive gastrointestinal tumor. In this study, clinical and gene data were collected to identify glycolytic genes that significantly associated with overall survival (OS) rate of CC patients through gene set enrichment analysis (GSEA) and Cox regression models. The K-M method was used to determine the difference of OS rate with high and low risk scores. The accuracy of risk scores were determined based on receiver operating characteristic (ROC) curve. Hierarch...
Muhammad Javed Iqbal1, Farah Rauf Shakoori1*, Bushra Muneer2 and Abdul Rauf Shakoori3
...t cancer patients. These tumors were classified into G1, G2 and G3 grades with an incidence of 12.5%, 42.5% and 45%, and grouped into the anatomic stages IIIA, IIIB and IIIC with 50%, 32.5% and 17.5% respectively. Further, molecular subtyping was done on the bases of cellular expression of hormone receptors by IHC and found 80% Luminal A, 12.5% Luminal B, 5% HER2 enriched and 2.5% Basal-like. Screening of mutations in PIK3CA, AKT1, MTOR and PTEN genes was carr...
Kanwal Nisa1, Sadia Roshan1, Shazia Shamas1,2*, Raheela Atta Mustafa3, Shamaila Irum1, Kalsoom Sughra4 and Memoona Iqbal1
...s by the evaluation of a tumor marker Prostate-specific antigen (PSA). Prevalence was recorded in Gujrat, Punjab, Pakistan. Blood samples of normal and prostate cancer males were taken from Gujrat, Punjab, Pakistan. Information including age, marital status, smoking, Prostate-specific antigen (PSA), testosterone, and any previous record was obtained with the patient’s consent. Exclusion and inclusion criteria were defined. The samples of control and pati...
Faisal Alzahani1* and Mohammed Abu El-Magd2*
...NPs) were used. The anti-tumor effects of CAPE and chitosan CNPs on cancer cells have been separately studied but the precise epigenetic molecular mechanisms for the combined therapy are still unclear. This study aimed to investigate the epigenetic mechanism of CAPE and/or CNPs on human HepG2 cells. The results revealed a significantly higher cytotoxic effect for CAPE on HepG2 cells than CNPs. The combined therapy with CAPE and CNPs exhibited significantly hig...
Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...ainst the expressions of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in the pulmonary section of mice induced with canine coronavirus (CCoV) vaccine. The research utilized male mice (Mus musculus) aged two to three months, divided into three groups: a control group administered with normal saline (D0), a CCoV-induced group (D1), and a group induced with CCoV along with 1 mL/kg body weight of Lactobacillus acidophilus for seven...
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
...t score (3), whereas the tumor necrosis factor alpha (TNF α) expression showed highest score (4) in the testes and epididymis tissue. The conclusion of the recent investigation found that tetrodotoxin has a harmful toxic effect on rats that indicated the effects of oxidative stress and increase interleukin1 (IL 1) and tumor necrosis factor alpha (TNF α) expression also induce hypospermatogenisis in testes and spe...
ASMA ZAFAR*, MONIBA KHAWAR, MAHNOOR CHAUDHRY, SAYYEDA SANA BATOOL, AMJAD HUSSAIN** & MUSHTAQ AHMAD SALEEM
...tion of azurin with P53 (tumor suppressor protein), Eph/ephrin receptor and Cadherin enhances the activity of azurin by inducing apoptosis in cancerous cells, disrupting signaling among cancerous cells and inhibiting the growth of cancerous cells as well as suppressing the activity of tumor cells. This review article focuses on the activity of the bacterial protein azurin as an anti-cancer agent and also the properties of az...
UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1
Aliya Irshad Sani1, Shumaila Usman2*, Zile Rubab1, Sufyan Ahmed3, Sana Iqbal3 and Zehra Ahmed4
...t recurrent and advanced tumors. Many immune markers are being evaluated in Oral squamous cell carcinoma (OSCC) including costimulatory molecules OX40 (Tumor necrosis factor receptor superfamily, member 4, TNFSRF4) and OX40L (tumor necrosis factor superfamily, member 4; TNFSF4). They function to potentiate T cells function to accentuate anti-tumor activi...
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
...-2 and enhanced the anti-tumor activity of HepG-2 as compared to MCF-7 cells. These conclusions suggest the potential use of the refolded cytokine as immunotherapeutic agent for treatment of hepatocellular carcinoma....
Yan Wang1,2*, Lin Liu3, Na Li4, Yijun Zong1, Wei Liu1, Wenhua Xu1, Qi Wang1, Peijuan Zhang1 and Huiling Feng2,5*
...lt; 0.05), and inhibited tumor formation in nude mice (p < 0.01). The protein expression levels of GLUT1, HK2, PFKL, ALDOA, PGK-1, PKM2 and LDHA were down-regulated (all p < 0.05), and the glucose consumption and the contents of lactic acid and ATP were decreased (all p<0.05). To conclusion by regulating the production of enzymes involved in aerobic glycolysis, LY6K may encourage the proliferation and energy metabolism of A549 cells....
Kang Cheng1*, Zhihua Song2, Jingyi Niu1, Jin Huang1, Laiting Liu1, Jinrong Wang1 and Yong Zhang1*
...ro-inflammatory cytokine tumor necrosis factor alpha at translational and transcriptional level, and the increased gene expression of interleukin 10 in the jejunum. In conclusion, fisetin alleviated the intestinal injury in rats caused by heat stress through inhibiting of oxidative stress and inflammation. This may offer a useful nutritional strategy for improving the health status of individuals exposed to heat stress....
Hafiz Muhammad Farooq Yaqub1, Sehrish Firyal1, Ali Raza Awan1, Muhammad Tayyab1, Rashid Saif2, Muti ur Rehman3, Muhammad Wasim1*
... ultimately resulting in tumor. Like human cancers, dogs have relatively high incidence of cancers, relatively large body size and responses to cytotoxic and other therapeutics. Small noncoding RNA having length of 22 base pair (bp) are called micro-RNA (miRNA), that are processed by Dicer from precursors with a characteristic hairpin secondary structure. miRNAs can regulate gene expression at the post-transcriptional level by binding to the 3′ untransla...
Hafiz Muhammad Farooq Yaqub1, Sehrish Firyal1, Ali Raza Awan1, Muhammad Tayyab1, Rashid Saif2, Muti ur Rehman3, Muhammad Wasim1*
... ultimately resulting in tumor. Like human cancers, dogs have relatively high incidence of cancers, relatively large body size and responses to cytotoxic and other therapeutics. Small noncoding RNA having length of 22 base pair (bp) are called micro-RNA (miRNA), that are processed by Dicer from precursors with a characteristic hairpin secondary structure. miRNAs can regulate gene expression at the post-transcriptional level by binding to the 3′ untransla...
*Arshad Javaid1, Iqra Haider Khan1 and Malik F. H. Ferdosi2
...ical activities viz. antitumor, antifungal, antibacterial, antioxidant,
anticancer, anti-inflammatory, antidiabetic, and analgesic.
...
Arshad Javaid1*, Malik F. H. Ferdosi2, Iqra Haider Khan1, Amna Shoaib1, Hafiz Muhammad Saeed2, Muhammad Abrar Ul Hassan1
...idant, antidiabetic, antitumor, etc....
Riam Yousfe Muttair
...nd the production of the tumor microenvironment in the fight against cancer. ...
Lei Chu1, Jie Yang2, Zhenshi Chen2, Xiajun Zhang1, Weidong Wu3*, Shaoru Zhang2 and Lihui Wang2*
...he most common malignant tumor in women, and its prognosis is poor. The key genes and pathways of CC need to be further discovered. GEO2R was used to identify differentially expressed genes (DEG), GO and KEGG enrichment were analyzed by DAVID. Then, the PPI network is constructed with strings. The HUB gene and module of DEGS were obtained by Cytoscape. Finally, GEPIA also analyzed the differential expression and survival of key genes. 234 DEG were extracted fr...
Xiaowei Zhang*
...e and CEA, CA125, CA19-9 tumor markers was 0.611, 0.603, 0.598, respectively, and all were significant. It was concluded that the combined treatment group of apatinib and trastuzumab can effectively intervene gastric cancer with ascites disease, reduce postoperative adverse reactions, promote cell apoptosis, especially the survival rate of long-term and short-term patients....
Islam S Alani*, Huda Sadoon Al-Biaty
...on to the advancement of tumors. The objective of this research was to evaluate the expression of PN in canine mammary gland tumors. The study material consists of 44 carcinomas and 6 benign tumors, together with 10 normal samples. Antibodies targeting the antigen POSTN have been used to conduct immunohistochemistry procedures. In normal biopsies, the expression of periostin was absent in ...
Quratulain Amjad1,2 and Abdul Rauf Shakoori1,2*
...in signaling cascades of tumor metastasis. In previous reports it is stated that the interactions between Slit2 and Robo1 are facilitated by heparan sulphate that is abundantly expressed on cell surface and extracellular matrix. Slit2 reduces tumor proliferation in lung cancer and glioblastoma cells whereas TGF-β is a well-described tumor inducer. The present study was aimed at deciph...
Shaza N. Alkhatib1, Rasha M. Ghoneim2, Hend M. Tag2,3, Hekmat M. Tantawy2, Heba M.A. Abdelrazek4*
...es transformation (LTT), tumor necrosis factor-alpha (TNF-α) and interleukins were assayed. Moreover, resistin, nitric oxide (NO), C-reactive protein (CRP), antinuclear antibodies (ANA) levels and cyclooxygenase-2 (COX-2) were estimated. Soy phytoestrogens diminished body weight gain (P<0.05), promoted total leukocytes count (P<0.05), abridged (P<0.05) the levels of interleukins, NO, CRP, resisting, ANA and COX-2 than control. Withdrawal of phyt...
Sherine Abbas1, Hadeer M. Shosha2, Hala M. Ebaid2, Heba Nageh Gad El-Hak2, Heba M. A. Abdelrazek3*
...tochemical expression of tumor necrosis factor (TNF) in liver and caspase-3 in kidney tissues were observed when the pregnant rats exposed to CO especially the high dose that were significantly altered than the low dose CO. These findings suggested that a higher dose treatment of CO to the mother can lead to adverse effects on the liver and kidneys. Nevertheless, the small dose treatment of CO to the mother resulted in less harm to them than the high dose.
Sherine Abbas1, Heba M.A. Abdelrazek2*, Eman M. Abouelhassan3, Nayrouz A. Attia4, Haneen M. Abdelnabi4, Seif El-Eslam E. Salah4, Abdelrahman M. Zaki4, Mohamed A. Abo- Zaid4, Nadia A. El-Fahla5
...actor kappa B (NFKB) and tumor necrosis factor-alpha (TNF-α) and histopathology for spleen and thymus were performed. Oral administration of neem extract to pregnant female rats caused significant (P<0.05) declines in birth and final body weights of male offspring and their thymus absolute weight (P<0.05). It also caused significant neutrophil (%) promotion (P<0.05) and lymphocyte (%) reduction (P<0.05), reduced serum TAC levels statistically...
