Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for...
Imed Ben Salem*, Aymen Ben Ibrahim, M’barek Chetoui and Said Nouira
...d length of the tympanic bulla, than the other species. This is probably related to a phenotypic evolution due to selective pressure, as is also the case in the genus Gerbillus....
Hamayun Khan1,*, Momen Khan2, Muhammad Subhan Qureshi3, Shakoor Ahmad1, Ali Gohar4, Hameed Ullah5, FarmanUllah6, Arab Hussain7, Pershotam Khatri8, Said Sajjad Ali Shah1, Hamid Rehman1 and Azmatullah Khan1
...act on the cryopreserved bull semen. Experiments were carried out on Achai breeding bulls of similar age at the Semen Production Unit, Harichand, District Charsadda, Khyber Pakhtunkhwa. Collection of semen was done using artificial vagina (42°C) for three weeks. Semen was extended in egg yolk extender at various inclusion levels (0.0%, 0.25%, 0.5%, 0.75 and 1.0%) of green tea extract. Testing was consummated for quality ...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
... from two mature buffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml ...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...mfortness experienced in bulls during semen collection by electro ejaculation method. The normal electro ejaculation method of semen collection (Method I) was compared to a modified method involving three stages of graduated electrical stimulation (Method II) in four crossbred bulls. The results showed that intensive muscle spasm, bull struggling and arc back were reduced (p ˂ 0.05), ...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
...
Nine healthy mature bulls having average age 3 ± 0.14 years, were selected in three different groups i.e., Sahiwal, Holstein-Friesian and crossbred (Sahiwal x Holstein-Friesian) to compare the semen quality. Semen samples were individually diluted with egg yolk extender and tested for individual motility, progressive motility, plasma membrane integrity, acrosomal and DNA integrity, seminal pH and dead sperm percentage in fresh and thawed sem...
Relationship of PIT-1 Gene Polymorphism with Breeding Parameters and Body Weights of Cows and Calves
Jarosław Pytlewski1, Ireneusz Antkowiak1 and Ewa Czerniawska-Piątkowska2,*
...-White Holstein-Friesian bulls and giving birth to single calf. The following information was taken from each animal: age at first insemination, age at first calving, number of semen portions attributable to successful insemination, length of pregnancy and periods of: insemination service, postpartum resting period, interpregnancy period and intercalving period. The study also determined weight of the cow and weight of the calf at calving (including gender). S...
İbrahim Aytekin1,*, Ecevit Eyduran2, Koksal Karadas3, Rifat Akşahan4 and İsmail Keskin1
...im, a total of 103 young bulls were used. In the prediction of fattening final live weight as an output variable, several continuous predictors evaluated in the current study were: withers height (WH), back height (BH), front rump height (FRH), back rump height (BRH), body length (BL), back rump width (BRW), chest depth (CD) and chest circumference (CC). Also, the breed factor was considered as a nominal predictor and fattening period (FP) was accepted as an o...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...sion levels in different bull testes tissues (1-day, 12-months-old and 24-months-old). In addition, genes mRNA and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay was performed to observe the cells proliferation. The results showed that miR-122 an...
Ewa Czerniawska-Piątkowska1,*, Magdalena Skibicka1, Barbara Cioch-Szklarz1, Jolanta Karakulska2, Karol Fijałkowski2 and Sonia Hiller3
... and traditional rearing bulls had a larger mass than heifer calves. In traditional rearing, results of analysis of the swabs from the nasal cavities revealed the presence of pathogenic or opportunistic bacteria at the level of the average number of micro-organisms (11-50 CFU) or high (> 50 CFU), which negatively affected the health of the calves. However, in cold rearing there was recorded absence or presence of microorganisms in the nasal cavity in a smal...
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
.../i>; Gyrodactylus turnbulli,); one digenean (Cryptocotyle sp.), two nematode (Capillaria sp., Camallanus sp.); two crustaceans (Argulus foliaceus and Lernaea cyprinacea,), one mollusca (Glochdium). The pathogenic fungal genera isolated were: Aspergillus, Alternaria, Penicillium, Mucor. Blastomyces, Rhizopus and Fusarium. Ten bacterial genera were also isolated; Aeromona...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...uality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination, pH was ...
M. Tariq Javed1, Nazir Ahmad2, Gulbeen Saleem3, Haleema Sadia4, Farkhanda Manzoor5 and Ghulam Hussain Dilbar
EFFECT OF AGE AND SEASON ON SEMEN CHARACTERISTICS OF BUFFALO BULL - A REVIEW
...en parameters in buffalo bull at different age and in different seasons has been published with conflicting reports. An effort is made to review the literature gathered from different sources on semen characteristics in buffalo bulls which will be helpful to researchers and allied professionals in future research or policy planning. The review is bifurcated in different sections for easy understanding of the facts. The revie...
Marian Kuczaj1, Ewa Czerniawska-Piątkowska2,*, Alicja Mizera1, Renata Pilarczyk2, Bogumiła Pilarczyk3 and Patryk Barton1
...country of origin of the bull (calf’s father). It was found that the high vitality of the calves is statistically significantly (at p ≤ 0.01) affects: the height at the back (˃ 150 cm) and the width in the hips of cows (˃ 63 cm). The Dutch and German fathers’ heifers were significantly smaller than other peers (at p ≤ 0.01). The mother’s weight before birth was significantly influenced by the daily increase in calves at the age of 1 m...
Zhaochao Deng1, Jingchen Chen1, Na Song2, Yongzhen Li3 and Zhiqiang Han1,*
Muamber Shah1, Muhammad Subhan Qureshi1, Rifat Ullah Khan1, Muhammad Mobashar1, Mubashar Ali Khalique2, Irfan Khattak3, Ambrina Tariq4, Ijaz Ahmad1 and Shabana Naz5,*
...emen quality of breeding bulls. A total of 8 adult (≥2 year) bulls (2 each from Jersey, Friesian, Sahiwal and cross bred) were selected with body condition score (BSC) at a scale of (0-5). The results showed that mean semen volume was significantly (P<0.05) high in Friesian bull. Sperm concentration and motility were significantly (P<0.05) high in Jersey b...
Naimat Ullah1, Sarzamin Khan2, Muhammad Mobashar3, Shahbaz Javaid4*, Tarique Tonio1 and Asif Mahmood5
...iesian Sahiwal crossbred bulls during summer season. A total of 24 Friesian Sahiwal crossbred bulls at nearly similar age and body weight were selected and allotted erratically into 3 treatment groups, denoted as A, B and C for a period of 2 months (May and June). Each group was assigned 8 bulls. Bulls in group A were not fed with any dietary fat (contro...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...ai and Holstein Friesian bull semen under the subtropical conditions of Peshawar region of Pakistan. Semen of six adult bulls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 42°C temperature. Gros...
Daniel Cocan1, Vioara Mireşan1, Florentina Popescu1, Radu Constantinescu1, Aurelia Coroian1, Călin Laţiu1, Romulus Valeriu Flaviu Turcu3, Alexandru Ştefan Fărcăşanu3 and Cristian Martonos2,*
...venomous glands in Brown bullhead (Ameiurus nebulosus) through MRI investigations. The main reason of this investigation was to provide a better understanding of the defence mechanism of this species. The presence, topography and structure of venomous glands made brown bullhead one of the top invasive species spreading its habitat worldwide. The specimens were collected from Stejeriş Lake, Cluj County. MRI investigat...
Muhammad Shuaib Khan1, Shakeeb Ullah1, Nisha. A.R2, Muhammad Umar3, Muhammad Inamullah Malik1*, Syed Muhammad Kamal Shah1, A. Zaman1, Shakir Ullah1, Imdad Ullah1, Sumera Ali Khan4
...imals i.e. camel, bull, horses/ mules and donkey. Score from female respondents by different pack animals i.e. camel, bull, horses/ mules and donkey receive were 33.67±2.55, 29±1.00, 29.52±2.52 and 58.67±2.082 respectively (% means ± Standard deviation), while from male respondents with the % means ± Standard deviation of 34.67±6.43, 35.00±1.00, 36.00&plus...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8µg/mL buserelin acetate (Buserelin&re...
Abdul Malik*, Abdul Sattar Shakir**, Muhammad Jamal Khan***, Muhammad Latif**, Zia-ul-Haq*
YIELD RESPONSE AND WATER USE EFFICIENCY OF SUGAR BEET IN SEMI-ARID ENVIRONMENT UNDER DIFFERENT DEFICIT IRRIGATION REGIMES
...ld was 32.02 tonees OO-J• Average sugar content for full irrigation was 14.14%, and for 60 % deficit irrigation sugar content was 16%. Where, the root water use efficiency was in the range of 8.13 for full irrigation to 10.87 kg m-3 for 60% deficit irrigation. The sugar water use efficiency was in the range of 1.15 for full irrigation to 1. 74 kg m-3 for 60% deficit irrigation. Yield response factor was in the range of 0.91 to 1.08. The relationship obtai...
Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2
A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...rs of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of buffalo milk was computed from 600 households, while 1,033 liters (43.7%) was used in households and 1,332 (56.3%) liters was sold. About 1,868 liters of cow milk were computed from 600 households, while 915 liters (49%) of milk used in households and 953 (51.0%) liters were sold. Majority of ...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...ion of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different concentr...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...ted from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion per mL conc., >60% motility) and cryopreserved using standard procedures. Percent sperm motility, liveability...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
...ize: small;">Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T...
Rao Muhammad Ramiz1, Arfan Ahmad2*, Aamir Ghafoor2, Muhammad Avais3, Qurat-ul-Ain1 and Muhammad Zahid Iqbal3
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...acteristics in crossbred bulls.
... 
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
... spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated thr...
Muhammad Waqas1*, Muhammad Shoaib2, Muhammad Saifullah1, Adila Naseem4, Sarfraz Hashim1, Farrukh Ehsan1, Irfan Ali3 and Alamgir Khan1
...ly observed at Garhi Habibullah station at 95% of the significance level. The flow duration curves (FDCs) of all basin stations showed that DTF performed better and is more effective than other AI techniques. R2, RMSE, and NSE assessed the performance evaluation. DTF was more efficient AI techniques with the average evaluation parameters R2, NSE, and RMSE are 0.998, 0.992, and 382 m3/sec. The assessment revealed that DTF has potential and may be considered as ...
Wen-Feng Li, Rong-Yue Zhang, Jiong Yin, Hong-Li Shan, Xiao-Yan Cang, Zhi-Ming Luo, Xiao-Yan Wang, Jie Li and Ying-Kun Huang*
...arcane borers, Abamectin•Bacillus thuringiensis and Tebufenozide were selected and applied for control of Sesamia inferens Walker and Argyroploce schistaceana (Snellen). The results showed that new sex pheromone trap of sugarcane borers in combination with Abamectin•Bacillus thuringiensis or Tebufenozide were the optimum mode for environmentally friendly control techniques of Sesamia ...
Ahmad Wahyudi1*, Sujono Sujono2, Listiari Hendraningsih2, Ari Prima2, Zane Vincēviča-Gaile3 and
Ivar Zekker4
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...cators of frozen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo
Tilahun Woretaw1, Netsanet Beyero2*, Yonatan Kassu3
..., heifers, calves, oxen, bull, sheep, goat, and equine, respectively. The drinking mixture of P. schimperiana in milk, and water was higher during the dry season and lowered during the wet season. The estimated biomass yields were 4.34±0.13 kg and 5.56±0.119 kg in the dry and wet seasons, respectively. Further feeding trial experiments should be conducted on live animals especially on a dairy animal for studying the response, for optimal producti...
Mohammed Sirajul Islam1,2*, Nurhusien Yimer1*, Wahid Haron1 , Faez Firdaus Abdullah Jesse1, Mark Hiew Wen Han1, Mamat-Hamidi Kamalludin3, Wan-Nor Fitri1, Ubedullah Kaka4, Abdul Quddus1,5
...K and KK × Brangus bulls. Ten (10) experimental bulls with similar ages were kept under the same paddock and fed with equal quality and quantity Guinea grasses and palm kernel cakes. Body weight was measured at monthly basis by using a digital animal weighing scale. Age at sexual maturity was determined by the time of semen ejaculation and quality. T...
Furqan Awan1,2, Sadia Nawaz3, Muhammad Muddassir Ali3,*, Asia Iqbal4, Raheela Akhtar5, Sehrish Firyal3, Haleema Sadia6, Arshad Javid4, Muhammad Rashid7, Muhammad Sulman Ali Taseer8 and Zhenling Zeng1
...ollowed by mongrel dogs (bully, n=23). In winter season, higher number of cases (n=34; from November to February) was recorded as compared to other seasons. To explore further important features, non-linear categorical PCA was employed. Prominently canine populations in the vicinity were found to be more varied. This is the first epidemiological study done in Pakistan that has identified some novel trends and circumstances regarding the CTVT....
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ealthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control where no Gallic acid was added. After the addition of extender, semen was cooled to 4°C, filled in 0.5mL straws for 4 h and frozen in liquid nitrogen at -196 °C. The parameters evaluated were pe...
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
...and high fertile buffalo-bulls. Also, the relationship between protein profile in BP of adult bulls and bull calves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected on...
Asim Faraz1*, Bernard Faye2, Cem Tirink3, Ayman Balla Mustafa4, Amal AlKharusi5, Morteza Bitaraf Sani6, Nasir Ali Tauqir7, Muhammad Arslan Akbar8, Muhammad Usman Saleem9, Rana Muhammad Bilal7, Abdul Waheed1, Muhammad Shahid Nabeel10
...se the available rutting bull in the seasonal breeder She-camels to shorten the age at puberty and calving interval and to get the offspring at desired time.
Novelty Statement | Due to climatic change, global warming issues and feed scarcity issues the camel will be the animal of future. But the camel production also faces some problems due to longer calving intervals, pregnancy periods, seasona...
Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy
...r weight of buffalo-male bulls.
Keywords | Buffalo-males, Blood, Testosterone, Leptin, Prolactin.
...
Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi
...im-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83± 13.57%, 53.63...
Nurul Isnaini*, Akhmad Kurniawan, Muhammad Nur Ihsan, Dedes Amertaningtyas, Artharini Irsyammawati, Faizal Andri
...0) and Brahman crossbred bulls (n=10) were raised under oil palm and cattle integrated farming system. Data were collected for cow and calf performance from two consequent calvings that were distributed into two groups according to the rainy days and rainfall for a year backward from the calving date. The rainy days were categorized as low (< 150 d) and high (≥ 150 d) whereas rainfall was categorized as low (< 2400 mm) and hig...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
...inematics of Bali-polled bull sperm to identify reproductive potential. Semen collection of Bali-polled bull was conducted once a week using an artificial vagina. The semen was proceeded until the frozen semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by usin...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...p>Administration GnRH to bulls is expected to improve semen quality. This study aims to determine the effect of giving the hormone GnRH in various doses on the quality of semen in Ongole Crossbreed (OC) bulls. The study used 18 OC bulls with three GnRH treatments, A (one dose/5ml/animal), B (half-dose/2.5ml/animal), and C without GnRH. The GnRH was administered once by intramuscular (IM) i...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
... preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as four groups (Control, G1, G2 and G3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progressive m...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...Polish Holstein-Friesian bulls. The research material was biological material (semen and blood) collected from 60 Holstein-Friesian bulls of the same age. The nucleotide substitutions analyzed are located in exon 4 of the AQP7 gene and are responsible for missense mutations. Individual genotypes were determined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viabi...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...roductive potency of the bull because it is related to the growth and development of livestock organs, including their reproductive organs. The purpose of the present study was to evaluate the characteristics of fresh and frozen semen quality of Aceh cattle at different ages. Three Aceh bulls were involved in this study. The semen was collected from each bull aged 6 to 10 years and the fre...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
...-thaw quality of Sahiwal bull spermatozoa. Qualifying semen ejaculates [motility (>60%) volume (>4ml) and concentration (>0.5 billion/ml)] collected from three Sahiwal bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C....
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...en of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were ...
Ahmad Zaman, Azhar Rafique*, Farhat Jabeen and Tayyaba Sultana
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
...tozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...quality of frozen-thawed bull sperm extended in Tris-buffered extender (TBE) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplementations were s...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
Saima Akter1, Md. Rasel Prank1, Shariful Islam1, Sharmin Akter1, Injamamul Hasnine1, Murshed Uddin Ahmed2, Md. Shohel Al Faruk3*
...s higher than in heifer, bull, and calf based on animal categories, and the animals within 2 years of age were more susceptible to parasitic infestation. As there are no previous studies on the parasitic infestation of cattle in Ullapara Upazila, therefore this study will provide a glimpse of present parasitic infestations in Upazila for future studies on this area.
Keywords | Fecal sample, Age, Trematode, Nematode, Protozoa.
...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...The development of local bull in many regions is currently leading to the development of local bulls and Indonesia is no exception, especially in South Sulawesi Province. One of local bulls in which developed for approximately last ten years is Bali-polled bull. Thus, the selection of polled bulls is crucial, mainly in...
Gulzhan Kalenovna Mussayeva1*, Alikhan Meldebekovich Meldebekov2, Nurgul Alikhanovna Meldebekova2, Gulshat Imanzhankyzy Shaykamal1, Batyrkhan Azimkhanovich Buralkhiyev2, Nurkuisa Mirzabekovich Rametov3 and Kanat Zhumanov4
... artificial insemination bulls from the line whose offspring are most productive and physiologically more adapted to the climatic, technological and forage conditions of the farm. When selecting, special attention should be paid to the strength of the hoofed horn and the duration of commercial use. For the given purposes it is recommended to use such bulls of the Wis Burke Ideal line whose average milk productivity exceeds t...
A.l. Abd El-Fattah; A.s. Saclik M.M. El-Kholi; I.A. Åbdcl-Hmnid and M.A. Madkour
...olecular studies of SCMV•E strain. a single protein band "ith a size of about 35-37 KDa was obtained after SDS-PAGE analysis of the purified virus preparation. The SCMV-E-RNA was isolated from virus-infected sugarcane tissues and then used as a template for reverse used transcription-polymerase to amplify the coat protein chain reaction (CP) gene (RT-PCR) Of SCMV-E to amplify strain. the ThecDNA that directl...
Gilyan V. Fedotova1,2, Marina I. Slozhenkina1, Ivan F. Gorlov1, Aizhan A. Kaidulina1, Alexander A. Mosolov1, Daria A. Mosolova1, Mohammadreza Poorghasemi3 and Alireza Seidavi3*
...Kazakh white-headed (IV) bulls were housed and fed under the same conditions. The bulls used were all classed as Elite. The feeding rate was intense. Steers in all groups were raised in the feedlot throughout the year. Growth rate of the crossbred steers was greater than the purebred steers. This was reflected in higher yields of the most valuable parts of the carcasses, the rump and saddle. Simmental crosses were distinguis...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...-Belgian-Blue-Crossbreed-bulls aged four years old were kept in a mooring cage and served with forage and concentrate (CP 16%) at 10% and 3% to the body weight, respectively. There were also added some egg yolk citrate diluent of 2.9% (v/v), Sodium citrate dihydrate buffer, 100 mL of distilled water, and an egg yolk mixture to a proportion of 20% egg yolk 8% (v/v) glycerol (Merck, Germany), penicillin (1,000 IU/mL), and streptomycin (1,000 mg/mL). Sperm was di...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
... weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with Tris-extender at each level of PEE (PEE0, PEE1, PEE2, PEE3, and PEE4). Semen was gradually diluted at 37 °C with each extender at a rate of 1: 10, equilibrated for 4 hours at 4 °C, packaged in French straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved p...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...ion using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen with -20 oC and growth factor concentrations were measured using the ELISA method. The result...
Annum Razzaq1*, Zia Ullah2, Arooj Naseer1 and Abdul Nasir Khalid1
El-Dougdoug2, Kh.A; Ghalyl, M.F. and Tahal , M.A.
...">An antiviral producing• Streptomyces species were isolated from soil rhizosphere in Zagazig province of Egypt. In order to identi$r the Streptomyces strains, morphological, physiological, biochemical and antagonism testes were performed. The Egyptian isolates of Streptomyces were found to be a species ofcalvus, canarius, vinaceusdrappus, nogalater and viridosporus. The Streptomyces spp were grown in glycerol asparagine broth medium and the culture super...
Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty
...ates are located in Euro•Asio-African lineage which contains Al H5Nl strains from Egypt, Turkey, Romania, Iraq, Mongolia, Iran, Korea and Nigeria and differed from those strains isolated in South-Eastern of Asia which contains Cambodia, Thailand, and Viet Nam Al H5Nl strains. The phylogenetic analysis of HA, NA, and Matrix (M) genes of Egyptian isolates of January 2007 from the same locality showed minor antigenic variation from those of 2006 and have bee...
Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman
...aster and more reliable • immune response against the H5N2 viral antigen after immunizing 3 weeks old chicken with a dose of 0.3 ml/bird, inoculated subcutaneously (s/c) in the nap of the neck, was fairly enough to evoke the required level of immunity. The approach through which this vaccine could be used as a possible potential vaccine candidate for protection of chickens against the highly pathogenic avian influenza (HPAI) virus has been discussed....
JEHAN A. M. GAFER.' HUSSEIN, H.A. and REDA I.M.
...cessive passages on MDBK•cells. The isolated viruses were then titerated and identified using different biological, serological and molecular assay....
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
...oplasmic small number of bullet shape particles.
Virological and pathological results were discussed.
...
Sahar A. Youssef I and A. A. Shalaby
...ible primer sets for one•step RT-PCR amplification used in a single closed tube to detect the presence of virus infection. Five fragments with different sizes corresponding to the different viral targets were amplified from infected samples, .i.e., 677, 172, 300, 270 and bp for ACLSV, PDV, PNRSV, PPV and ToRSV respectively, Results showed that multiplex PCR products of the expected sizes were obtained for all virus isolates assayed they were tested ...
Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar
...without ribavirin (RBV) •to examine its combination effect. With an electric shock treatments alone (5, 10 and 15 mA electric current), the replicates become PVY-free in rates53.8,72.7 and 87.5%, respectively. Simultaneous, RBV and electric shock had higher efficiency for PVY elimination, reaching rates of healthy plantlets of 66.7% with 5 mA, 90.0% with 10 mA, and 100.0% with 15 mA....
Rahmat Rahmat1, Muhammad Yusuf2*, Abdul Latief Toleng2, Herdis Herdis3, Athhar Manabi Diansyah2, Hasrin Hasrin4
...ovement patterns in Bali bull sexed sperm using freeze-dry albumin. This study was conducted five times for semen collection and using treatments; T1 = Medium concentration of 10% and 30%, T2 = Medium concentration of 15% and 45%, T3 = Medium concentration of 20% and 60%. Parameters measured in this study were motility, viability, abnormality, membrane integrity, acrosome integrity, movement pattern and proportion of spermatozoa. The data obtained were analyse...
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
...e concentrations in Aceh bull. Aceh bull was used in this study and allotted into two treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2α (Lutalyse™, dinoprost, tromethamine) 1 hour before semen collection, while in treatment 2 (P2), the semen collection was carried out 2 hours after PGF2α administration. The treatment was performed five times in alternate we...
UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR
...addition, radicals (DPPH• & ABTS•+) scavenging potentials were also determined. Results revealed that drying methods have no significant influence on recovery of antioxidants from sample matrix. However, methanolic extracts exhibited highest antioxidant potential that confirmed superiority of methanol as extraction media over the other solvents used. In addition, microwave-assisted extraction technique for the ...
Ebban Bagus Kuntadi1, and Amam Amam2*
...ing heifers, steers, and bulls. The observation involved 4,238 Brahman Cross cattle. The data were analyzed using descriptive analysis and independent sample t-test using SPSS 26.0 software. The results revealed that the weight loss of the imported cattle on the MV Diamantina ship was 12.92 ± 5.66 kg per cattle, Barkly Pearl was 9.98 ± 5.05 kg per cattle, and MV Gudali Ex was 14.32 ± 4 .20 kg per cattle, while the weight loss of Brahman Cr...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...maintain the motility of bull sperm during the cooling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to ...
Hilarius Yosef Sikone1*, Gomera Bouk2, Edelnia Kristina Bere2, Yohana Kamlasi2, Elisabeth Yulia Nugraha1
...70 (cow calf) and 16.21 (bull calf), for weaning weights of 107.43 (cow calf) and 109.37 (bull calf), and for the weight of mothers aged > 4-5 years, 248.11 the income of cattle breeders is greatest for breeders with the TDKT system reach IDR 7,137,737.00, followed by the SIMK system, which the breeder’s income amounting to IDR 5,377,735.00, the SLMK system provides an income of IDR 4,692,278.00, and finally, the TD...
Abdul Rahman Sesay1,2, Muhammad Saif-ur-Rehman1*, Faisal Ramzan1 and Faisal Saeed Awan3
...ample size of 98 Sahiwal bulls. The genotyping of all animals was conducted using the BovineHD140k BeadChip. After undergoing quality control measures, 87 samples as well as 74,070 SNPs located throughout 29 autosomes were selected as well as included in the study. The selection signatures were examined using the iHS and the Tajima D approach. The result reveals the current positive selections on BTA 1, 2, 6, 11, 12, 15, 17, 21, and 27 with the iHS test, while...
Hanif Gul and G. A. Bajwa
Mahmood Iqbal Sheikh
...ed. Heavy machinery like bulldozers was put to
the maximum use to level the land for agriculture and
afforesation. Hither to unemployed young and the old got
job opportunities in mills, factories, construction and
afforestation works. Land lying waste for ages was
turned into highly productive agricultural fields, fruit
orchards and tree plantations. Education and medical
facilities were brought at the door step of villagers.
Although this programme gr...
Editor
...ituary: Late Khawaja Habibullah, DFO, Azad Kashmir Forest Department and Late Sir Harry G. Champion, C.I.E....
Bashir Hussain Shah and M. Ismail Chaudhry
...roject area at Garhi Habibullah, Distt. Hazara. Nine genera of ectoparasitic nematodes and some free living nematodes were also recovered. The percentage of the plant parasitic nematodes of the genera, Rotylenchus Paratylenchus Pratylenchus, Helicotylenchus and Tylenchorhynchus was quite high amongist the parasitic nematodes extracted from the soil and samples of Eucalyptus saplings at Chichawatni and Garhi Habibullah. Other...
Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*
...ost-thawed semen of Bali bull. This research was conducted at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada. Seventy-five semen straws produced from one bull were divided into 3 groups; each group consisted of 25 semen straws as long as 3, 6, and 8 months of storage in liquid nitrogen. The quality of thawed semen included motility, viability, abnormality, plasma memb...
Nasir Ali Tauqir1*, Asim Faraz2, Muhammad Imran Babar3 and Irfan Shahzad Sheikh3
...der in Nili Ravi buffalo bulls. Four cannulated buffalo bulls were used for in situ screening of oat fodder harvested at 100 days of age sown using different levels of phosphorous and phosphorous plus nitrogen fertilizers. Dry matter (DM) and crude protein (CP) content were higher (p<0.05) in oat fodder supplemented with P and NP fertilizer as compared to control. The supplementation of P fertilizer resulted in decreased ...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...-thawed Tropical Brahman bull sperm. Sperm samples were obtained and pooled from eight sexually mature Tropical Brahman Bull, separated into seven equal proportions, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each α-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membrane plasma were analyz...
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
...ity of Tharparkar cattle bull semen. The research was carried out at Department of Animal Reproduction, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agricultural University Tandojam. In this study four fertile Tharparkar bulls (A, B, C and D) having age of 4-5 years were used. A total of 52 (n=13) ejaculates were collected with the help of artificial vagina, after the collection macroscopic (color, volume and p...
Al-Moataz Bellah Mahfouz Shaarawy1, Mahmoud Yassin Mohamed1*, Mahmoud Sayed Sayah2,1, Ashraf Ali Mehany1, Ezzat Arafa Ahmed El-Beltagi1, Shimaa M. Ali1
... testosterone levels for bulls developed earlier than the 1st group. Heifers in the same group were superior in reproductive performance such as reaching puberty, and fertility compared to other groups. The 3rd group showed the highest concentrations of serum proteins, glucose, and lipid profiles. The 2nd group came next, and 1st group registered the lowest concentrations. In conclusion, data on GH and LP levels from birth to weaning could be a useful early-li...
Aulia Puspita Anugra Yekti, Ani Atul Arif, Putri Utami, Habib Asshidiq Syah, Anggita Dian Pramudhita, Rr. Ani Rizqianti, Trinil Susilawati*
...diluents in Belgian Blue bull. The research material used was a semen sample of Belgian Blue cross bull with progressive motility of fresh semen of≥ 70%. The research used a Completely Randomized Design (CRD) with four treatments and ten replications. Data were analyzed using the Tukey test. The treatment in the study was T0: sexing PDGC 10 fractions using Andromed® diluent. T1: sexing PDGC 10 fractions using tris ami...
Nguyen Ba Trung1,2*, Pham Thi Kim Phuong1,2
...articipate in the famous bull-racing festival. A small number of cattle in the Phum squirrels have a pure Ongole phenotype. However, due to the practice of castration of male calves and Sindization, purebred Ongole are in danger of extinction, whereas genetic features have not been investigated. This study aimed to examine the genetic features of Ongole cattle by utilizing molecular markers. We examined the nucleotide sequences of mitochondrial DNA and the SRY...
Devi Ermawati1, Panjono2, Sigit Bintara1, Ali Agus3, Budi Prasetyo Widyobroto2, Bertha Yudistyra4, Rifai Mustofa4, Tety Hartatik1*
...cattle with Belgian Blue bullsand Brahman Cross dams, and ten samples of Brahman Cross cattle grown at PT. Widodo Makmur Perkasa were taken. Estrogen levels and visual indicators of estrus were tracked both during and 30 days after AI. Both Brahman Cross and F1 Crossbred cattle showed strong estrus signals during AI, with estrus signs of 125.50±5.64 for Brahman Cross and 128.30±5.41 for F1 Crossbred, decreasing to 120.80±6.05 a...
Adi Tiya Warman1, Panjono1*, Tri Satya Mastuti Widi1, Sigit Bintara1, Bayu Andri Atmoko2, Endang Baliarti3
...ttle farmers in choosing bull semen for artificial insemination (AI) in Lombok Tengah Regency, West Nusa Tenggara, Indonesia. The bull semen choice was classified into three categories: Bali cattle, exotic cattle, and both. Respondent selection was carried out using a purposive sampling method, with 95 respondents. Data were collected through interviews using a structured questionnaire that had been tested for validity and r...
Van Dai Nguyen1, ¥, Van Ba Nguyen2, ¥, Thi Thanh Nhan Giang2, Cong Dinh Nguyen2, Thi Lan Nguyen1, Dinh Ngoan Vu1, Duc Chuyen Nguyen1, Van Can Ta1, Khac Khanh Nguyen2, Thi Lien Cao2, Doan Lan Pham2, Khanh Van Nguyen2, Ngoc Tan Nguyen3*
...o LB sub-populations and bull exchange policy among populations requires further study.
Keywords | Bubalus bubalis, Genetic divergence, Native buffalo, Microsatellite marker, Polymorphism, Swamp buffalo
... 
